CN219991473U - Reaction tube magnetic frame of protein purification instrument - Google Patents
Reaction tube magnetic frame of protein purification instrument Download PDFInfo
- Publication number
- CN219991473U CN219991473U CN202321527171.0U CN202321527171U CN219991473U CN 219991473 U CN219991473 U CN 219991473U CN 202321527171 U CN202321527171 U CN 202321527171U CN 219991473 U CN219991473 U CN 219991473U
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- Prior art keywords
- reaction tube
- adjusting block
- protein purification
- electromagnet
- reaction
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- 238000001742 protein purification Methods 0.000 title claims abstract description 18
- 239000011324 bead Substances 0.000 claims abstract description 13
- 238000001179 sorption measurement Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000746 purification Methods 0.000 description 14
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000012264 purified product Substances 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The utility model discloses a reaction tube magnetic frame of a protein purification instrument, which comprises a bracket body and is characterized in that: a plurality of reaction tube placing grooves are distributed on the support body, the reaction tube placing grooves are used for placing reaction tubes, an electromagnet is arranged on one side of each reaction tube placing groove, and the electromagnet is used for adsorbing magnetic beads in the reaction tubes on the tube walls of the reaction tubes. The magnetic rack is specially tailored for the reaction tube, and is especially used for supporting and placing 50mL reaction tube; the electromagnet is arranged on the arc-shaped limit baffle with the adjustable position, so that the arc-shaped limit baffle has the function of limiting and fixing the reaction tube, and the position of the electromagnet can be adjusted, thereby realizing excellent magnetic bead adsorption effect; each electromagnet is provided with an independent power switch and an indicator lamp, so that the magnetic bead adsorption operation of a single reaction tube and the indicating function of the working state can be realized, and the operation is greatly facilitated.
Description
Technical Field
The utility model relates to life science experimental equipment, in particular to a reaction tube magnetic frame of a protein purification instrument.
Background
The protein purifier is used in large life science laboratory, and compared with traditional method, the protein purifier has raised purifying efficiency and saving in manpower and material resources. The protein purifier can perform various common purification techniques, such as affinity chromatography, ion exchange chromatography, hydrophobic chromatography, gel filtration, etc. Thus, this instrument can support purification of various proteins, including recombinant proteins, antibodies, vaccines or native proteins, and the like. Conventional protein purifiers are typically designed for plate purification, most commonly using micro deep well 96-well plates. The design can solve the purification scheme of multiple sample volumes, but the purification volume of each sample is limited by the volume of the pore plate, which is generally 1 mL/sample at maximum, and the purification requirement of a large-volume sample can not be met. If a large volume of sample is equally divided into each hole for purification, the purified products of a plurality of holes are collected and concentrated after purification, and a great deal of time is needed.
Conventional protein purifiers are typically designed for plate purification, most commonly using micro deep well 96-well plates. The design can solve the purification scheme of multiple sample volumes, but the purification volume of each sample is limited by the volume of the pore plate, which is generally 1 mL/sample at maximum, and the purification requirement of a large-volume sample can not be met. If a large volume of sample is equally divided into each hole for purification, the purified products of a plurality of holes are collected and concentrated after purification, and a great deal of time is needed.
Disclosure of Invention
The utility model aims to provide a reaction tube magnetic frame of a protein purification instrument, which is specially used for a reaction tube.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a reaction tube magnetic force frame of protein purification appearance, includes the support body, and it has a plurality of reaction tube standing grooves to distribute on the support body, and the reaction tube standing groove is used for placing the reaction tube, every one side of reaction tube standing groove all is equipped with the electro-magnet, the electro-magnet for adsorb the intraductal magnetic bead of reaction on the pipe wall of reaction tube.
Further, a soft rubber pad is arranged at the lower end of the reaction tube placing groove.
Further, a plurality of power switches are arranged on the top surface of the support body, the plurality of power switches are respectively in one-to-one correspondence with the plurality of reaction tube placing grooves, and the power switches are used for controlling the power switches of the electromagnets on one side of the corresponding reaction tube placing grooves.
Further, a plurality of power indicator lamps are arranged on the top surface of the support body and correspond to the reaction tube placing grooves one by one, and when the power indicator lamps are on, the electromagnet on one side of the reaction tube placing groove corresponding to the power indicator lamps is in a working state.
Further, the bottom of the support body is wide, the top of the support body is narrow, one side surface of the support body is an inclined surface, and the plurality of reaction tube placing grooves are positioned on the inclined surface.
Further, the two sides of the reaction tube placing groove are respectively provided with a left sliding rail and a right sliding rail, the reaction tube placing groove further comprises an arc-shaped limiting baffle, the two ends of the arc-shaped limiting baffle are respectively fixed with a left sliding adjusting block and a right sliding adjusting block, the left sliding adjusting block and the right sliding adjusting block are respectively arranged on the left sliding rail and the right sliding rail, the left sliding adjusting block and the right sliding adjusting block are respectively provided with a left locking screw and a right locking screw, and the left locking screw and the right locking screw are respectively used for locking and fastening the left sliding adjusting block and the right sliding adjusting block on the left sliding rail and the right sliding rail; the arc-shaped limiting baffle is provided with the electromagnet.
Further, a left dovetail chute and a right dovetail chute are respectively arranged on the left sliding adjusting block and the right sliding adjusting block, and the left dovetail chute and the right dovetail chute are respectively arranged on a left inverted trapezoid bar of the left sliding rail and a right inverted trapezoid bar of the right sliding rail.
Further, the reaction tube placing grooves are provided with 8 reaction tube placing grooves.
Further, the reaction tube placing groove is used for placing 50mL of reaction tubes.
The utility model has the beneficial effects that:
the magnetic rack is specially tailored for the reaction tube, and is especially used for supporting and placing 50mL reaction tube;
the electromagnet is arranged on the arc-shaped limit baffle with the adjustable position, so that the arc-shaped limit baffle has the function of limiting and fixing the reaction tube, and the position of the electromagnet can be adjusted, thereby realizing excellent magnetic bead adsorption effect;
each electromagnet is provided with an independent power switch and an indicator lamp, so that the magnetic bead adsorption operation and the indication function of the working state of a single reaction tube can be realized, and the operation is greatly facilitated.
Drawings
The utility model will be further described with reference to the accompanying drawings, in which embodiments do not constitute any limitation of the utility model, and other drawings can be obtained by one skilled in the art without inventive effort from the following figures:
FIG. 1 is a schematic structural diagram of embodiment 1 of the present utility model;
FIG. 2 is a top view of the device shown in FIG. 1;
FIG. 3 is a schematic view of the arc-shaped limit stop of FIG. 1;
FIG. 4 is a schematic structural diagram of embodiment 2 of the present utility model;
fig. 5 is a schematic structural view of the arc-shaped limit baffle shown in fig. 4.
In the figure: 1. a bracket body; 2. an inclined plane; 3. a reaction tube placement tank; 4. a left slide rail; 5. a right slide rail; 6. an arc-shaped limit baffle; 7. a left slip adjusting block; 8. a right slip adjusting block; 9. a left locking screw; 10. a right locking screw; 11. a left dovetail chute; 12. a right dovetail chute; 13. a left inverted trapezoidal bar; 14. right inverted trapezoidal bar; 15. an electromagnet; 16. a top surface; 17. a power switch; 18. a power indicator light; 19. soft rubber cushion.
Detailed Description
In order to make the technical solution of the present utility model better understood by those skilled in the art, the present utility model will be described in further detail with reference to the accompanying drawings and the specific embodiments, and it should be noted that the embodiments of the present utility model and features in the embodiments may be combined with each other without conflict.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper surface", "lower surface", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "forward rotation", "reverse", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplify the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present utility model.
Example 1
As shown in fig. 1, 2 and 3, a reaction tube magnetic rack of a protein purification instrument comprises a bracket body 1, wherein 8 reaction tube placing grooves 3 are distributed on the bracket body 1, the reaction tube placing grooves 3 are used for placing reaction tubes, and soft rubber pads 19 are arranged at the bottom of the lower end grooves of the reaction tube placing grooves 3 and used for supporting the lower ends of the reaction tubes on the soft rubber pads 19. The device also comprises 8 electromagnets 15, the 8 electromagnets 15 are in one-to-one correspondence with 8 reaction tube placing grooves 3, and the electromagnets 15 are arranged on the lower side of the reaction tube placing grooves 3 and are used for adsorbing magnetic beads in the reaction tube onto the tube wall of the reaction tube. The magnetic rack is specially tailored for the reaction tube, and is especially used for supporting and placing 50mL reaction tube.
The bottom of the support body 1 is wide, the top is narrow, one side surface of the support body 1 is an inclined surface 2, 8 reaction tube placing grooves 3 are distributed on the inclined surface 2, a left sliding rail 4 and a right sliding rail 5 are respectively arranged on two sides of the reaction tube placing grooves 3, the support further comprises an arc-shaped limiting baffle 6, a left sliding adjusting block 7 and a right sliding adjusting block 8 are respectively fixed on two ends of the arc-shaped limiting baffle 6, the left sliding adjusting block 7 and the right sliding adjusting block 8 are respectively arranged on the left sliding rail 4 and the right sliding rail 5, a left locking screw 9 and a right locking screw 10 are respectively arranged on the left sliding adjusting block 7 and the right sliding adjusting block 8, and the left locking screw 9 and the right locking screw 10 are respectively used for locking and fixing the left sliding adjusting block 7 and the right sliding adjusting block 8 on the left sliding rail 4 and the right sliding rail 5; the 8 arc-shaped limit baffles 6 are provided with the electromagnets 15, and the purpose of adjusting the positions of the electromagnets 15 is achieved by adjusting the positions of the arc-shaped limit baffles 6. The left sliding adjusting block 7 and the right sliding adjusting block 8 are respectively provided with a left dovetail chute 11 and a right dovetail chute 12, and the left dovetail chute 11 and the right dovetail chute 12 are respectively arranged on a left inverted trapezoid bar 13 of the left sliding rail 4 and a right inverted trapezoid bar 14 of the right sliding rail 5. The arc-shaped limiting baffle has the function of limiting and fixing the reaction tube.
The top surface 16 of the bracket body 1 is provided with 8 power switches 17, the 8 power switches 17 are respectively in one-to-one correspondence with the 8 reaction tube placing grooves 3, and the power switches 17 are used for controlling the power switch of the electromagnet 15 on one side of the corresponding reaction tube placing groove 3.
The top surface 16 of the bracket body is provided with 8 power indicator lamps 18, the 8 power indicator lamps 18 are respectively in one-to-one correspondence with the 8 reaction tube placing grooves 3, and when the power indicator lamps 18 are on, the electromagnet 15 on one side of the reaction tube placing groove 3 corresponding to the power indicator lamps 18 is in a working state. Each electromagnet is provided with an independent power switch and an indicator lamp, so that the magnetic bead adsorption operation and the indication function of the working state of a single reaction tube can be realized, and the operation is greatly facilitated.
Example 2
As shown in fig. 4 and 5, the reaction tube magnetic rack of the protein purification apparatus of this embodiment 2 is different from that of embodiment 1 in that the mounting positions of the electromagnets 15 are different, in this embodiment 2, 8 electromagnets 15 are respectively mounted on 8 arc-shaped limit baffles 6, and the purpose of adjusting the positions of the electromagnets 15 is achieved by adjusting the positions of the arc-shaped limit baffles 6. The arc-shaped limit baffle 6 not only has the function of limiting and fixing the reaction tube, but also can adjust the position of the electromagnet, thereby realizing excellent magnetic bead adsorption effect.
Working principle: the reaction tubes on the reaction tube magnetic rack of the protein purification instrument are 50mL reaction tubes, and total of 8 reaction tubes can simultaneously purify 8-channel samples at one time. Firstly, placing a reaction tube into a reaction tube placing groove 3 on a bracket body 1, and then adjusting the position of an arc-shaped limit baffle 6 to adjust the specific position of an electromagnet 15 so as to realize the optimal suction force on magnetic beads; meanwhile, the reaction tube is limited and fixed through the arc-shaped limiting baffle 6.
When the liquid waste in a certain reaction tube needs to be extracted, a power switch 17 corresponding to the reaction tube is firstly turned on, a power indicator lamp 18 corresponding to the power switch 17 is turned on, and at the moment, the electromagnet 15 is electrified to generate magnetism, so that all the magnetic beads leave the bottom in the reaction tube and are adsorbed on the tube wall of the reaction tube 3 by the electromagnet 15; then, the lower end of the liquid discarding suction head stretches into the bottom of the reaction tube, the liquid discarding pump is started, the liquid discarding in the reaction tube is pumped into the waste liquid bottle, after the liquid suction is completed, the liquid discarding suction head is lifted and removed, and the power switch 17 is immediately turned off, so that the magnetic field is removed, and the magnetic beads are prevented from agglomerating in the magnetic field for a long time.
Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction. While embodiments of the present utility model have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the utility model, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the utility model.
Claims (9)
1. The utility model provides a reaction tube magnetic force frame of protein purification appearance, includes the support body, its characterized in that: a plurality of reaction tube placing grooves are distributed on the support body, the reaction tube placing grooves are used for placing reaction tubes, an electromagnet is arranged on one side of each reaction tube placing groove, and the electromagnet is used for adsorbing magnetic beads in the reaction tubes on the tube walls of the reaction tubes.
2. The reaction tube magnetic rack of the protein purification instrument according to claim 1, wherein: the lower end of the reaction tube placing groove is provided with a soft rubber pad.
3. The reaction tube magnetic rack of the protein purification instrument according to claim 2, wherein: the top surface of the support body is provided with a plurality of power switches, the power switches are respectively in one-to-one correspondence with the reaction tube placing grooves, and the power switches are used for controlling the power switches of the electromagnets on one side of the corresponding reaction tube placing grooves.
4. A reaction tube magnetic rack of a protein purification apparatus according to claim 3, wherein: the top surface of the support body is provided with a plurality of power indicator lamps which are respectively in one-to-one correspondence with the plurality of reaction tube placing grooves, and when the power indicator lamps are on, the electromagnet on one side of the reaction tube placing groove corresponding to the power indicator lamps is in a working state.
5. The reaction tube magnetic rack of the protein purification instrument according to claim 1, wherein: the bottom of the support body is wide, the top is narrow, one side of the support body is an inclined plane, and the plurality of reaction tube placing grooves are positioned on the inclined plane.
6. The reaction tube magnetic rack of the protein purification instrument according to claim 5, wherein: the two sides of the reaction tube placing groove are respectively provided with a left sliding rail and a right sliding rail, the reaction tube placing groove further comprises an arc-shaped limiting baffle, the two ends of the arc-shaped limiting baffle are respectively fixed with a left sliding adjusting block and a right sliding adjusting block, the left sliding adjusting block and the right sliding adjusting block are respectively arranged on the left sliding rail and the right sliding rail, the left sliding adjusting block and the right sliding adjusting block are respectively provided with a left locking screw and a right locking screw, and the left locking screw and the right locking screw are respectively used for locking and fastening the left sliding adjusting block and the right sliding adjusting block on the left sliding rail and the right sliding rail; the arc-shaped limiting baffle is provided with the electromagnet.
7. The reaction tube magnetic rack of the protein purification instrument according to claim 6, wherein: the left sliding adjusting block and the right sliding adjusting block are respectively provided with a left dovetail chute and a right dovetail chute, and the left dovetail chute and the right dovetail chute are respectively arranged on a left inverted trapezoidal bar of the left sliding rail and a right inverted trapezoidal bar of the right sliding rail.
8. The reaction tube magnetic rack of the protein purification instrument according to claim 1, wherein: the reaction tube placing grooves are 8.
9. The reaction tube magnetic rack of the protein purification instrument according to claim 1, wherein: the reaction tube placing groove is used for placing 50mL of reaction tubes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321527171.0U CN219991473U (en) | 2023-06-15 | 2023-06-15 | Reaction tube magnetic frame of protein purification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321527171.0U CN219991473U (en) | 2023-06-15 | 2023-06-15 | Reaction tube magnetic frame of protein purification instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219991473U true CN219991473U (en) | 2023-11-10 |
Family
ID=88612482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321527171.0U Active CN219991473U (en) | 2023-06-15 | 2023-06-15 | Reaction tube magnetic frame of protein purification instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219991473U (en) |
-
2023
- 2023-06-15 CN CN202321527171.0U patent/CN219991473U/en active Active
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