CN219409748U - Nucleic acid nano magnetic bead separation device - Google Patents

Abstract

The utility model discloses a nucleic acid nano magnetic bead separating device which comprises an upper pipe groove seat, wherein the bottom end of the upper pipe groove seat is clamped with a lower pipe groove seat, a hose is clamped between the bottom end of the upper pipe groove seat and the lower pipe groove seat, a sliding hole is formed in the top end of the upper pipe groove seat, a rotating block is rotatably arranged at the top end of the upper pipe groove seat.

Description

Nucleic acid nano magnetic bead separation device

Technical Field

The utility model relates to the technical field of nucleic acid extraction equipment, in particular to a nucleic acid nano magnetic bead separation device.

Background

With the continuous development of biotechnology, people need to extract nucleic acid from biological samples for research, magnetic bead separation is a common mode of nucleic acid extraction, by utilizing superparamagnetism of silica nano microspheres, DNA and RNA in samples can be separated under the actions of guanidine hydrochloride, guanidine isothiocyanate and the like and an externally applied magnetic field, in a 'nucleic acid nano magnetic bead separation device and separation method' of China patent with the application number of 201710248091. X, a structure of a tube groove seat and a tube groove cover is arranged, a permanent magnetic strip is arranged in an upper tube groove on the tube groove cover, the permanent magnetic strip can be ensured to adsorb magnetic beads in a mixed solution in a hose on the inner wall of the hose, the whole side surface of the permanent magnetic strip is an effective area for separating the magnetic beads, the magnetic field adsorption separation area can be expanded into the whole side surface of the permanent magnetic strip, and the separation efficiency can be improved;

however, in the currently used nucleic acid nano magnetic bead separating device, when the upper groove seat and the lower groove seat are clamped, the position of the hose is unstable, so that the external pressure loss of the hose is easily caused, and the follow-up operation is influenced.

Disclosure of Invention

The utility model provides a nucleic acid nano magnetic bead separation device, which can effectively solve the problems that the prior nucleic acid nano magnetic bead separation device is unstable in hose position fixation and easy to cause external pressure loss of a hose and influence subsequent operation when an upper groove seat and a lower groove seat are clamped.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a nucleic acid nanometer magnetic bead separator, includes the pipe socket, the bottom joint of going up the pipe socket has lower pipe socket, the joint has the hose between the bottom of going up the pipe socket and the lower pipe socket, the sliding hole has been seted up on the top of going up the pipe socket, the rotating block is installed in the top rotation of going up the pipe socket, the depression bar is installed on the top of rotating block, the inside joint of sliding hole has the slide bar, the connecting plate is installed on the top of slide bar, the bar hole has been seted up at the middle part of connecting plate, the arc cardboard is installed to the bottom of slide bar, the standing groove has been seted up to the bottom of going up the pipe socket;

the utility model discloses a pipe joint, including upper pipe groove seat, division board, spacer rod, fixing bolt, the division board is all installed to the both ends of upper pipe groove seat, a plurality of arc groove has been seted up to the bottom of division board, the spacer rod is installed to the bottom of division board, the top threaded connection of division board has fixing bolt.

Preferably, a plurality of arc clamping grooves are formed in the bottom end of the arc clamping plate, and the arc clamping grooves are connected with the outer portion of the hose in a clamping mode.

Preferably, two sliding holes and two sliding rods are respectively arranged, and the two sliding rods are respectively in sliding clamping connection with the inside of the sliding holes.

Preferably, the upper pipe groove seat and the partition plate are fixedly arranged through a fixing bolt;

the separation rod is arranged at two ends of the arc-shaped groove.

Preferably, the bottom of down tub seat is connected with the braced frame, the connecting hole has been run through on the top of braced frame, the inside slip joint of connecting hole has the bracing piece, the bottom of bracing piece is installed supporting baseplate, the vibrator is installed on the top of supporting baseplate, electric telescopic handle is installed to supporting baseplate's top both ends symmetry.

Preferably, the input ends of the vibrator and the electric telescopic rod are electrically connected with the output end of an external power supply;

the top end of the electric telescopic rod is connected with the supporting frame.

Compared with the prior art, the utility model has the beneficial effects that: the utility model has scientific and reasonable structure and safe and convenient use:

1. through set up sliding hole, the rotating block, the depression bar, the slide bar, the connecting plate, the bar hole, the arc cardboard, the standing groove, the division board, arc groove and division bar, move the slide bar downwardly, the depression bar gets into bar hole middle part, rotate the depression bar, carry out the joint fixedly to the connecting plate, arc cardboard and standing groove separation, go up tube holder and arc cardboard and move downwardly, the arc cardboard earlier with hose joint, then rotate the depression bar and remove the restriction to the connecting plate, be difficult for dislocation when can helping hose middle part joint through the joint in advance, convenient timely adjustment, when last tube holder moves downwardly, aim at the arc groove with the hose, make the division bar joint respectively in the both sides of corresponding hose, thereby when last tube holder moves downwardly, can make hose both ends keep in stable position.

2. Through setting up carriage, connecting hole, bracing piece, supporting baseplate and electric telescopic handle, during the vibration, the vibrator is laminated with down the tube holder bottom, and the power of switch-on vibrator drives down the tube holder vibration, and after the vibration operation was accomplished, the power of switch-on electric telescopic handle, electric telescopic handle drive carriage and shift up, will down the tube holder upwards promote, and lower tube holder and vibrator separation make the hose can eliminate the vibration fast, make things convenient for follow-up operation.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model.

In the drawings:

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is an exploded view of the present utility model;

FIG. 3 is a schematic view of the structure of the partition plate of the present utility model;

FIG. 4 is a schematic view of the structure of the arc-shaped clamping plate of the present utility model;

FIG. 5 is a schematic view of the structure of the placement tank of the present utility model;

FIG. 6 is a schematic view of the structure of the support floor of the present utility model;

reference numerals in the drawings: 1. an upper pipe groove seat; 2. a lower pipe groove seat; 3. a hose; 4. a sliding hole; 5. a rotating block; 6. a compression bar; 7. a slide bar; 8. a connecting plate; 9. a bar-shaped hole; 10. an arc-shaped clamping plate; 11. a placement groove; 12. a partition plate; 13. an arc-shaped groove; 14. a partition rod; 15. a fixing bolt; 16. a support frame; 17. a connection hole; 18. a support rod; 19. a support base plate; 20. a vibrator; 21. an electric telescopic rod.

Detailed Description

The preferred embodiments of the present utility model will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present utility model only, and are not intended to limit the present utility model.

Examples: as shown in fig. 1-6, the utility model provides a technical scheme, the nucleic acid nano magnetic bead separation device comprises an upper tube seat 1, a lower tube seat 2 is clamped at the bottom end of the upper tube seat 1, a hose 3 is clamped between the bottom end of the upper tube seat 1 and the lower tube seat 2, a sliding hole 4 is formed in the top end of the upper tube seat 1, a rotating block 5 is rotatably arranged at the top end of the upper tube seat 1, a compression bar 6 is arranged at the top end of the rotating block 5, a sliding rod 7 is clamped inside the sliding hole 4, two sliding rods 4 and 7 are respectively arranged, the two sliding rods 7 are respectively and slidably clamped inside the sliding hole 4, connection of the sliding rods 7 is facilitated, a connecting plate 8 is arranged at the top end of the sliding rod 7, a strip-shaped hole 9 is formed in the middle of the connecting plate 8, an arc clamping plate 10 is arranged at the bottom end of the sliding rod 7, a plurality of arc clamping plates 10 are formed in arc clamping grooves and are clamped with the outer part of the hose 3, and a placing groove 11 is formed in the bottom end of the upper tube seat 1;

the division board 12 is all installed at the both ends of going up pipe groove seat 1, and a plurality of arc groove 13 has been seted up to the bottom of division board 12, and separation pole 14 is installed in the both ends of arc groove 13, conveniently separates hose 3, and the top threaded connection of division board 12 has fixing bolt 15, goes up through fixing bolt 15 fixed mounting between pipe groove seat 1 and the division board 12, makes things convenient for the fixed of division board 12.

The bottom of lower pipe seat 2 is connected with braced frame 16, connecting hole 17 has been seted up in the top of braced frame 16 running through, the inside slip joint of connecting hole 17 has bracing piece 18, braced bottom plate 19 is installed to the bottom of bracing piece 18, vibrator 20 is installed on the top of braced bottom plate 19, electric telescopic handle 21 is installed to the top both ends symmetry of braced bottom plate 19, vibrator 20 and electric telescopic handle 21's input all with external power source's output electric connection, electric telescopic handle 21's model is TGA-24, electric telescopic handle 21's top is connected with braced frame 16, conveniently promote braced frame 16 to remove.

The working principle and the using flow of the utility model are as follows: firstly, when in use, a sample and magnetic beads are placed in a hose 3, the hose 3 is placed at the top end of a lower pipe groove seat 2, then a sliding rod 7 is moved downwards, a pressing rod 6 enters the middle part of a strip-shaped hole 9, the pressing rod 6 is rotated, a connecting plate 8 is clamped and fixed through the pressing rod 6, and an arc-shaped clamping plate 10 is separated from a placing groove 11;

then the upper pipe groove seat 1 and the arc clamping plate 10 are downwards moved, the arc clamping plate 10 is firstly clamped with the hose 3, then the compression bar 6 is rotated to release the limit on the connecting plate 8, and finally the upper pipe groove seat 1 is downwards moved to be clamped with the lower pipe groove seat 2, so that the middle part of the hose 3 is not easy to misplace during clamping through the early clamping, and the adjustment is convenient in time;

when the upper pipe groove seat 1 moves downwards, the hose 3 is aligned with the arc-shaped groove 13, and the separation rods 14 are respectively clamped at two sides of the corresponding hose 3, so that when the upper pipe groove seat 1 moves downwards, the two end parts of the hose 3 can be kept at stable positions;

when the vibrator 20 is used, the vibrator 20 is attached to the bottom end of the lower pipe groove seat 2, the power supply of the vibrator 20 is connected, the lower pipe groove seat 2 is driven to vibrate, after the vibration operation is finished, the power supply of the electric telescopic rod 21 is connected, the electric telescopic rod 21 drives the supporting frame 16 to move upwards, the lower pipe groove seat 2 is pushed upwards, the lower pipe groove seat 2 is separated from the vibrator 20, the hose 3 can quickly eliminate vibration, and follow-up operation is facilitated.

Finally, it should be noted that: the foregoing is merely a preferred example of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (5)

1. The utility model provides a nucleic acid nanometer magnetic bead separator, includes upper tube groove seat (1), its characterized in that: the bottom joint of going up tube holder (1) has lower tube holder (2), joint has hose (3) between the bottom of going up tube holder (1) and lower tube holder (2), slide hole (4) have been seted up on the top of going up tube holder (1), rotating block (5) are installed in the top rotation of going up tube holder (1), depression bar (6) are installed on the top of rotating block (5), slide bar (7) have been clamped in the inside of slide hole (4), connecting plate (8) are installed on the top of slide bar (7), bar hole (9) have been seted up at the middle part of connecting plate (8), arc cardboard (10) are installed to the bottom of slide bar (7), standing groove (11) have been seted up to the bottom of going up tube holder (1);

the two ends of the upper pipe groove seat (1) are respectively provided with a partition plate (12), the bottom ends of the partition plates (12) are provided with a plurality of arc-shaped grooves (13), the bottom ends of the partition plates (12) are provided with partition rods (14), and the tops of the partition plates (12) are connected with fixing bolts (15) in a threaded manner;

the bottom of lower tube holder (2) is connected with braced frame (16), connecting hole (17) have been run through on the top of braced frame (16), inside slip joint of connecting hole (17) has bracing piece (18), supporting baseplate (19) are installed to the bottom of bracing piece (18), vibrator (20) are installed on the top of supporting baseplate (19), electric telescopic handle (21) are installed to the top both ends symmetry of supporting baseplate (19).

2. The nucleic acid nano magnetic bead separation device according to claim 1, wherein a plurality of arc-shaped clamping grooves are formed in the bottom end of the arc-shaped clamping plate (10), and the arc-shaped clamping grooves are clamped with the outside of the hose (3).

3. The nucleic acid nano magnetic bead separation device according to claim 1, wherein two sliding holes (4) and two sliding rods (7) are respectively arranged, and the two sliding rods (7) are respectively in sliding clamping connection with the inside of the sliding holes (4).

4. The nucleic acid nano magnetic bead separation device according to claim 1, wherein the upper tube seat (1) and the separation plate (12) are fixedly arranged through a fixing bolt (15);

the separation rod (14) is arranged at two ends of the arc-shaped groove (13).

5. The nucleic acid nano magnetic bead separation device according to claim 1, wherein the input ends of the vibrator (20) and the electric telescopic rod (21) are electrically connected with the output end of an external power supply;

the top end of the electric telescopic rod (21) is connected with the supporting frame (16).