CN216877958U

CN216877958U - Affinity chromatography hollow column

Info

Publication number: CN216877958U
Application number: CN202123111362.2U
Authority: CN
Inventors: 叶伟
Original assignee: Wuhan Chunyuan Biotechnology Co ltd
Current assignee: Wuhan Chunyuan Biotechnology Co ltd
Priority date: 2021-12-13
Filing date: 2021-12-13
Publication date: 2022-07-05
Anticipated expiration: 2031-12-13

Abstract

The application discloses affinity chromatography hollow column belongs to chromatography equipment technical field, including the glass hollow tube, the inside of glass hollow tube is provided with down places the dish, the top of placing the dish down is provided with places the dish, the up end of placing the dish down has seted up a set of the same first slot, place down on the dish seted up with the first spacing spout that first slot is linked together, on place the inner wall fixedly connected with connecting strip of dish, the inside central point of connecting strip puts fixedly connected with fixed column, the second slot has been seted up at the top of fixed column, the lateral wall of fixed column seted up two with the second slot is to the spacing spout of second of intercommunication. This application has the effect that the person of facilitating the use takes out the inside sieve of affinity chromatography empty column.

Description

Affinity chromatography hollow column

Technical Field

The application relates to the technical field of chromatography equipment, in particular to an affinity chromatography hollow column.

Background

The affinity chromatography hollow column generally refers to a glass tube used for affinity chromatography experiments, and is a chromatography method for separating molecules by utilizing the binding property of a stationary phase, the affinity chromatography is also called affinity chromatography, molecules with certain binding capacity with a substance to be separated are connected on a gel filtration chromatography column, the binding of the molecules is reversible, the molecules can be separated from each other when the condition of a mobile phase is changed, the affinity chromatography can be used for purifying or concentrating a certain molecule from a mixture, also can be used for removing or reducing the content of a certain molecule in the mixture, one pair of ligands (also called ligands) capable of reversibly binding and separating biological molecules is used as a ligand and is coupled with a solid phase carrier with large aperture and hydrophilicity to prepare a specific affinity adsorbent, the affinity adsorbent is used for filling the chromatography column, when the mixture containing the separated substance flows through the chromatography column along with the mobile phase, the ligand on the affinity adsorbent selectively adsorbs substances capable of being combined with the ligand, other proteins and impurities are not adsorbed, the substances to be separated and the ligand are desorbed by using a proper buffer solution, and the purified target product can be obtained.

However, the sieve plate in the existing affinity chromatography hollow column is not easy to take out, and is troublesome to use; thus, the existing requirements are not satisfied.

SUMMERY OF THE UTILITY MODEL

In order to make things convenient for the user to take out the inside sieve of affinity chromatography empty column, this application provides an affinity chromatography empty column.

The application provides an affinity chromatography empty column adopts following technical scheme:

an affinity chromatography hollow column comprises a glass hollow tube, wherein a lower placing plate is arranged inside the glass hollow tube, an upper placing plate is arranged above the lower placing plate, a group of same first slots are formed in the upper end face of the lower placing plate, a first limiting sliding groove communicated with the first slots is formed in the lower placing plate, a connecting strip is fixedly connected to the inner wall of the upper placing plate, a fixing column is fixedly connected to the center position inside the connecting strip, a second slot is formed in the top of the fixing column, and two second limiting sliding grooves communicated with the second slots are formed in the outer side wall of the fixing column.

Furthermore, the bottom of the glass hollow tube is fixedly connected with a suction tube, and the lower end of the suction tube is in threaded connection with a sealing threaded head.

Further, the inner wall fixedly connected with spacing ring of placing the dish down, the up end of spacing ring is provided with down the sieve, the top of connecting strip is provided with and is annular last sieve.

Furthermore, two lubricating films are adhered to the outer side walls of the lower placing disc and the upper placing disc.

Furthermore, the bottom of the placing plate is fixedly connected with a clamping strip which is in plug-in fit with the first slot, and the clamping strip is in sliding fit with the first limiting sliding groove.

Furthermore, a long bar is arranged above the fixed column and is close to the end face of the fixed column, and a fixed strip is fixedly connected to the end face of the fixed column.

In summary, the present application includes at least one of the following advantageous technical effects:

(1) two groups of first slots and first limiting sliding grooves are formed in the lower placing plate, second slots and second limiting sliding grooves are formed in the fixing columns, fixing strips on the long strip rods are inserted into the second slots, the long strip rods are rotated, the fixing strips are rotated into the second limiting sliding grooves, the second limiting sliding grooves play a role in limiting the fixing strips, two clamping strips at the bottom of the upper placing plate are inserted into the first slots in the lower placing plate, the upper placing plate is rotated, the clamping strips are rotated into the first limiting sliding grooves communicated with the first slots, the long strip rods move upwards, so that the lower placing plate and the upper placing plate can be simultaneously drawn out from a glass hollow tube, the integral operation is simple, and meanwhile, the disassembly is convenient;

(2) through place the dish under and paste lubricated film with the lateral wall of placing the dish on, lubricated film plays certain sealed effect, conveniently places the dish down simultaneously and places the dish on and reciprocate, has promoted the perfectness of device greatly.

Drawings

FIG. 1 is a cross-sectional view of an affinity chromatography column;

FIG. 2 is an enlarged view of a portion of the area A in FIG. 1;

fig. 3 is a schematic structural view of the lower and upper trays.

The reference numbers in the figures illustrate:

1. a glass empty tube; 2. a suction tube; 3. sealing the thread head; 4. a lower placing tray; 5. an upper placing tray; 6. A limiting ring; 7. a lower sieve plate; 8. a first slot; 9. a first limiting chute; 10. lubricating the rubber sheet; 11. A connecting strip; 12. fixing a column; 13. a second slot; 14. a second limiting chute; 15. an upper sieve plate; 16. Clamping the strip; 17. a long bar; 18. and (4) fixing the strip.

Detailed Description

The technical solution in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application; it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments, and all other embodiments obtained by those of ordinary skill in the art without any inventive work based on the embodiments in the present application belong to the protection scope of the present application.

In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

The present application is described in further detail below with reference to the attached drawings.

The embodiment of the application discloses an affinity chromatography hollow column, please refer to fig. 1 and combine fig. 2 and fig. 3, a suction pipe 2 is fixedly connected to the bottom of a glass hollow pipe 1, a sealing screw head 3 is connected to the lower end thread of the suction pipe 2, a lower placing plate 4 is arranged inside the glass hollow pipe 1, an upper placing plate 5 is arranged above the lower placing plate 4, a group of identical first slots 8 is arranged on the upper end face of the lower placing plate 4, a first limiting chute 9 communicated with the first slots 8 is arranged on the lower placing plate 4, a connecting strip 11 is fixedly connected to the inner wall of the upper placing plate 5, a fixed column 12 is fixedly connected to the central position inside the connecting strip 11, a second slot 13 is arranged on the top of the fixed column 12, two second limiting chutes 14 communicated with the second slot 13 are arranged on the outer side wall of the fixed column 12, a limiting ring 6 is fixedly connected to the inner wall of the lower placing plate 4, the upper end surface of the limiting ring 6 is provided with a lower sieve plate 7, the top of the connecting strip 11 is provided with an annular upper sieve plate 15, the bottom of the upper placing plate 5 is fixedly connected with a clamping strip 16 which is in inserted fit with the first slot 8, the clamping strip 16 is in sliding fit with the first limiting sliding slot 9, a long strip rod 17 is arranged above the fixed column 12, the end surface of the long strip rod 17 close to the fixed column 12 is fixedly connected with a fixed strip 18, the fixed strip 18 on the long strip rod 17 is inserted into a second slot 13 on the fixed column 12, then the long strip rod 17 is rotated, the fixed strip 18 is rotated into two second limiting sliding slots 14 on the outer side of the fixed column 12, the second limiting sliding slots 14 play a role of limiting the fixed strip 18, the long strip rod 17 rotates to drive the upper placing plate 5 to rotate, the two clamping strips 16 at the bottom of the upper placing plate 5 are inserted into the first slot 8 on the lower placing plate 4, and the clamping strips 16 are rotated into the first limiting sliding slots 9 communicated with the first limiting sliding slots 8 through the rotation of the upper placing plate 5, because first spacing spout 9 can block card strip 16, with rectangular pole 17 rebound alright with place down set 4 and place set 5 on with and take out from glass empty tube 1 simultaneously, whole easy operation can be fast with placing down set 4 and place set 5 on and take out.

Referring to fig. 3, the outer side walls of the lower placing tray 4 and the upper placing tray 5 are both adhered with two lubricating films 10, the lubricating films 10 have a certain sealing effect, and the lower placing tray 4 and the upper placing tray 5 can be conveniently moved up and down.

The implementation principle of the affinity chromatography hollow column in the embodiment of the application is as follows: by placing the lower sieve plate 7 on the limit ring 6 on the inner wall of the lower placing tray 4 and then sleeving the upper sieve plate 15 on the fixed column 12 of the upper placing tray 5, when the lower placing plate 4 and the upper placing plate 5 need to be taken out, the fixing strip 18 on the long rod 17 is inserted into the second slot 13 on the fixed column 12, then the long rod 17 is rotated, the fixing strip 18 is rotated into the two second limiting sliding grooves 14 on the outer side of the fixed column 12, because the second limit sliding groove 14 limits the fixed strip 18 to rotate, the long strip rod 17 rotates to drive the upper placing plate 5 to rotate, the two clamping strips 16 at the bottom of the upper placing plate 5 are inserted into the first slot 8 on the lower placing plate 4, the clamping strip 16 is rotated into the first limit sliding groove 9 communicated with the first slot 8 through the rotation of the upper placing plate 5, since the first limiting sliding groove 9 can clamp the clamping strip 16, the lower placing plate 4 and the upper placing plate 5 can be simultaneously drawn out of the glass hollow tube 1 by moving the long rod 17 upwards.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. An affinity chromatography empty column comprises a glass empty tube (1), and is characterized in that: the glass hollow tube is characterized in that a lower placing plate (4) is arranged inside the glass hollow tube (1), an upper placing plate (5) is arranged above the lower placing plate (4), a group of identical first slots (8) are formed in the upper end face of the lower placing plate (4), a first limiting sliding groove (9) communicated with the first slots (8) is formed in the lower placing plate (4), a connecting strip (11) is fixedly connected to the inner wall of the upper placing plate (5), a fixing column (12) is fixedly connected to the center of the inside of the connecting strip (11), a second slot (13) is formed in the top of the fixing column (12), and two second limiting sliding grooves (14) communicated with the second slot (13) are formed in the outer side wall of the fixing column (12).

2. An affinity chromatography column according to claim 1, wherein: the bottom fixedly connected with suction tube (2) of glass air traffic control (1), the lower extreme threaded connection of suction tube (2) has sealed screwed head (3).

3. An affinity chromatography column according to claim 1, wherein: the inner wall fixedly connected with spacing ring (6) of placing dish (4) down, the up end of spacing ring (6) is provided with down sieve (7), the top of connecting strip (11) is provided with and is annular last sieve board (15).

4. An affinity chromatography column according to claim 1, wherein: two lubricating films (10) are adhered to the outer side walls of the lower placing disc (4) and the upper placing disc (5).

5. An affinity chromatography column according to claim 1, wherein: the bottom of the placing plate (5) is fixedly connected with a clamping strip (16) which is in plug-in fit with the first slot (8), and the clamping strip (16) is in sliding fit with the first limiting sliding groove (9).

6. An affinity chromatography column according to claim 1, wherein: a long bar (17) is arranged above the fixed column (12), and a fixed strip (18) is fixedly connected to the end face, close to the fixed column (12), of the long bar (17).