CN204018008U - A kind of heating oscillating magnetic flux separator - Google Patents
A kind of heating oscillating magnetic flux separator Download PDFInfo
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- CN204018008U CN204018008U CN201420401915.9U CN201420401915U CN204018008U CN 204018008 U CN204018008 U CN 204018008U CN 201420401915 U CN201420401915 U CN 201420401915U CN 204018008 U CN204018008 U CN 204018008U
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Abstract
The utility model discloses a kind of heating oscillating magnetic flux separator, comprise three primary modules, i.e. Magneto separate module, heating module and vibration module.Magneto separate module comprises linear electric motors, leading screw, magnet carrier, permanent magnet, and heating module comprises electromagnetism load push-pull, spring, gripper shoe, support column, heating tank, heating film, vibration module involving vibrations motor, eccentric hammer.This device drives magnet carrier to move on leading screw by linear electric motors, thus drives moving up and down of permanent magnet, and the relative position controlling permanent magnet and microwell plate realizes reunion and the dispersion of magnetic nanoparticle; Promote gripper shoe by electromagnetism load push-pull to move up and down, and then drive heating tank to move up and down by support column, the distance controlling heating tank and microwell plate hole wall realizes the function heating and close.Finally achieve function complete, small and exquisite and be easy to the integrated biological specimen treating apparatus based on Magneto separate, utilize robot arm device can realize the automation of whole experimentation.
Description
Technical field
The utility model relates to biological specimen treatment technology, particularly a kind of high flux full automatic heating oscillating magnetic flux separator.
Background technology
Biological specimen is varied, has the gas of blood plasma, serum, whole blood, lymph, saliva, various tissue, hair, urine, bile, tear, spinal fluid, sweat, milk, amniotic fluid, ight soil and exhalation.Biological specimen is studied to the pre-treatment work first will carrying out biological specimen, the disposable operation carrying out rapidly and efficiently to great amount of samples of usual needs, therefore, suitable biological specimen treatment and analyses equipment can alleviate the workload of researcher greatly, operating efficiency is provided, better advances the development of subject.
Along with the develop rapidly of nanometer science and technology, nano material is applied to life science gradually, and the research and development for life science provides new technology and means.Wherein, the preparation and application of magnetic nanoparticle become study hotspot.Magnetic nanoparticle has that specific area is large, superparamagnetism, be easy to the advantages such as finishing, is widely used in biomedical sector, and wherein, it is one of its important application in this field that magnetic nanoparticle is used for that biological specimen is separated.During magnetic nanoparticle separation of biomolecules, have that separating rate is fast, efficiency is high, reusable, simple to operate, easy practical function, easily realize automation and the feature such as special physicochemical properties and biocompatibility such as the activity not affecting separate substance.Further, use magnetic nanoparticle advantage on Aulomatizeted Detect very outstanding: traditional separation method is as phenol-chloroform method, Chelexl00 method, salting out method etc., there is liquid-liquid to be separated, the step such as centrifugal, centrifugation apparatus is bulky, complex operation, required time is long, is not suitable for the demand being applied to Aulomatizeted Detect.And compared with above method, using magnetic nanoparticle lock out operation simple, required time is short, when externally-applied magnetic field, magnetic nanoparticle is magnetized, and is attracted on magnetic pole, during magnetic field dissipate, the magnetic on magnetic nanoparticle also disappears simultaneously, is again dispersed in reactant liquor.Utilize magnetic nanoparticle to carry out the operation such as separation and location of biological specimen, more easily realize integrated and automation.
Magnetic nanoparticle is widely used in the detection etc. of fixing and DNA of the separation of cell, immunoassays, protein and enzyme, biological specimen processing method based on Magneto separate comprises steps such as adding sample, lysis, adsorption of DNA, Magneto separate, heating mixing usually, corresponding self-reacting device needs to have the functions such as application of sample, Magneto separate, heating and vibration, comprises mechanical arm, static-magnetic separator, heater, vibration evenly mixing device and other some functional devices.At least corresponding station of each device, complete the process of biological specimen process, mechanical arm needs to complete microwell plate transfer work between above-mentioned multiple station repeated multiple timesly, substantially increases workload, bring inconvenience to experiment, considerably increase the possibility of cross pollution simultaneously.Further, this quasi-instrument is unfavorable for small modular and is integrated in the Automation workstation of large-scale one.
Utility model content
Technical problem: for the deficiencies in the prior art, the utility model provides a kind of full automatic heating oscillating magnetic flux separator, magnetic separating device, temperature control equipment and vibration evenly mixing device are combined into one, microwell plate bracket and permanent magnet, heating arrangements, oscillating mechanism are provided, the function of Magneto separate, heating or vibration can be realized easily, possess integrality, compact, facilitate integrated advantage.The process of biological specimen can be completed in experimentation without the need to mobile microwell plate, solve and repeatedly shift that complex operation, cross pollution probability that microwell plate brings are high, the defect of poor controllability, and be integrated into shortcoming bulky in Automation workstation.
Technical scheme: above-mentioned technical problem, the utility model provides a kind of heating oscillating magnetic flux separator, and this device comprises three primary modules, i.e. Magneto separate module, heating module and vibration module, and auxiliary body;
Magneto separate module comprises linear electric motors, leading screw, magnet carrier, permanent magnet; Heating module comprises electromagnetism load push-pull, gripper shoe, spring, support column, heating tank, heating film; Vibration module involving vibrations motor, eccentric hammer; Auxiliary body comprises base, fixed head, 4 elasticity pillars, main frames;
Described base is groove-like, and centre is provided with three holddown grooves, is respectively used to fixing linear electric motors, electromagnetism load push-pull and vibrating motor, and middle two lateral processes place two pieces of fixed heads; Described fixed head corner is provided with 4 elasticity pillar holddown grooves, and described elasticity pillar is placed in vertical in holddown groove;
Described main frame is placed on 4 elasticity pillars, and described main frame center lower end connects an eccentric hammer, and eccentric hammer is connected with vibrating motor;
Minor face both sides, described main frame top are two fixed terminals, and the built-in ball of described fixed terminal, for fixing microwell plate, makes microwell plate be in same position all the time; Described fixed terminal lays respectively at two minor faces of microwell plate, thus does not affect the crawl of mechanical arm to microwell plate;
Described leading screw one end is fixed on linear electric motors, in vertically placing, described leading screw connects magnet carrier, magnet carrier stretches out the support of a cuboid, and support is provided with 4 magnetic patch holes, places 4 pieces of permanent magnets, permanent magnet is cuboid, parallel placement between permanent magnet, realizes the reunion of magnetic nanoparticle at sidewall by one between pipe array adjacent on microwell plate in the corresponding 4 pieces of permanent magnets of difference;
Described electromagnetism load push-pull is fixed a gripper shoe, in gripper shoe, 2 support columns are fixed in both sides, and 2 support columns are positioned at parallel placement on same level line, and described spring is positioned at lower end in the middle of gripper shoe, and be in the middle of two support columns, heating tank is fixed on support column top;
Described heating tank is an irregular cuboid, is smooth heating surface outside two long limit, places a heating film respectively; Heating tank has 8 contact surfaces, the every a line in one_to_one corresponding microwell plate array, and contact surface is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, and realization is seamless applying with hole wall; Heating tank top is the via hole of adapt trickle orifice plate, makes microwell plate can put into heating tank and fits with contact surface; Aperture on the corresponding microwell plate of aperture on heating tank top, can accelerate heat transfer.
Preferably, the contact surface in described heating tank and described permanent magnet are in the different arrays into microwell plate, and both do not collide.
Beneficial effect:
(1) the utility model overcomes the existing deficiency based on single device simple function in the biological specimen process automation equipment of Magneto separate, magnetic separating device, heater and vibrating device are integrated in a device, in abundant guarantee microwell plate solution Magneto separate, fully mixing and uniform temperature under incubation with heat, ensured the integrality of function.
(2) the utility model is in the whole course of work, microwell plate containing reaction solution is static to be all the time fixed on above main frame, single device achieves the operation of Magneto separate, heating and vibration, so both save working space and make the effect that device is small and exquisite, ensure that biological specimen process, the interference between microporous pipe can be avoided again, greatly prevent cross pollution;
(3) modularized design, in this device, Magneto separate, heating and vibration module all can independently use, and do not interfere with each other;
(4) practical, this device is Open architecture, have frame for movement simple, be skillfully constructed and be easy to the features such as realization, accessible site is on the devices such as large-scale biochemical analyzer, also can independently use as miniaturized biological specimen treating apparatus, such device meets the actual demand of user.
Accompanying drawing explanation
Fig. 1 is monnolithic case figure of the present utility model;
Fig. 2 is component front view of the present utility model;
Fig. 3 is component rearview of the present utility model;
Fig. 4 is bottom schematic view of the present utility model;
Fig. 5 is magnet carrier schematic diagram of the present utility model;
Fig. 6 is heating tank schematic diagram of the present utility model;
In figure: base 101, electromagnetism load push-pull 102, fixed head 103, elasticity pillar 104, main frame 105, ball 106, fixed terminal 107, heating tank 108, heating film 109, vibrating motor 201, linear electric motors 202, leading screw 203, magnet carrier 204, permanent magnet 205, gripper shoe 206, support column 208, eccentric hammer 301, heating surface 501, contact surface 502, via hole 503, aperture 504.
Detailed description of the invention
Below in conjunction with drawings and the embodiments, the utility model patent is described in further detail:
A kind of heating oscillating magnetic flux separator, comprises three primary modules, i.e. Magneto separate module, heating module and vibration module.Magneto separate module comprises linear electric motors, leading screw, magnet carrier, permanent magnet, and heating module comprises electromagnetism load push-pull, spring, gripper shoe, support column, heating tank, heating film, vibration module involving vibrations motor, eccentric hammer.In addition, the supplementary structures such as base, fixed head, elasticity pillar and main frame are also comprised.
Described base is groove-like, and mid portion fixes linear electric motors, electromagnetism load push-pull and vibrating motor, and middle two lateral processes place two pieces of fixed heads.
Described fixed head corner is provided with 4 elasticity pillar holddown grooves, and described elasticity pillar is placed in vertical in holddown groove;
Described main frame is placed on 4 elasticity pillars, and described main frame center lower end connects an eccentric hammer, and eccentric hammer is connected with vibrating motor; Minor face both sides, described main frame top are two fixed terminals, and the built-in ball of described fixed terminal, for fixing microwell plate, makes microwell plate be in same position all the time.Described fixed terminal lays respectively at two minor faces of microwell plate, thus does not affect the crawl of mechanical arm to microwell plate;
Described leading screw one end is fixed on linear electric motors, in vertically placing, described leading screw connects magnet carrier, magnet carrier is provided with the draw-in groove of 4 permanent magnets, place 4 block permanent magnets, permanent magnet is cuboid, parallel placement between permanent magnet, can place a permanent magnet between pipe array adjacent on microwell plate;
Described electromagnetism load push-pull is fixed a gripper shoe, in gripper shoe, 2 support columns are fixed in both sides, and 2 support columns are positioned at parallel placement on same level line, and described spring is positioned at lower end in the middle of gripper shoe, and be in the middle of two support columns, heating tank is fixed on support column top.Described heating tank is an irregular cuboid, is smooth heating surface outside its two long limit, places a heating film respectively; Heating tank has 8 contact surfaces, the every a line in one_to_one corresponding microwell plate array, and contact surface is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, and realization is seamless applying with hole wall.Heating tank top is the via hole of adapt trickle orifice plate, makes microwell plate can put into heating tank and fits with contact surface; Aperture on the corresponding microwell plate of aperture on heating tank top, can accelerate heat transfer.
Device of the present utility model, drives magnet carrier to move on leading screw by linear electric motors, thus drives moving up and down of permanent magnet, and the relative position controlling permanent magnet and microwell plate hole wall realizes reunion and the dispersion of magnetic nanoparticle; Promote gripper shoe by electromagnetism load push-pull to run up and down, and then drive heating tank to move up and down by support column, the distance controlling heating tank and microwell plate hole wall realizes the function heating and close; Controlling eccentric hammer by vibrating motor drives main frame to realize the vibrating function of whole device.Finally, the automation mechanized operation of whole nucleic acid extraction can be completed by the different sequential of control Magneto separate, vibration, heating.
Further, in the utility model, after the energising of electromagnetism load push-pull, produce magnetic field, drive gripper shoe move upward, make heating tank and microwell plate hole wall paste with, heating tank is metal material (gold, silver, aluminium, iron etc.), the heating film energising of both sides, utilizes metal heat-conducting principle to realize the temperature conduction of heating tank, realizes the heating function of microwell plate.After the power-off of electromagnetism load push-pull, magnetic field dissipate, drive gripper shoe to move downward, be generally 3mm, heating tank is separated with microwell plate, and the power-off of heating film, closes heating function.
Further, in the utility model, the described spring of heating module plays the effect supporting and cushion heating tank, high vibration when preventing electromagnetism load push-pull from moving up and down.
Further, in the utility model, described elasticity pillar plays effect that is fixing and buffering.Vibrating motor energising rear drive eccentric hammer drives main frame of reciprocating vibration, thus drives the vibration of the microwell plate be fixed on the fixed terminal of main frame top, stops vibration after closing vibrating motor.
Further, in the utility model, linear electric motors drive leading screw to drive magnet carrier to move upward, and make 4 block permanent magnets in magnet carrier between the every two perform hole walls of microwell plate array, realize the reunion of magnetic nanoparticle; When magnet carrier moves downward, 4 block permanent magnets are positioned at microwell plate array lower end, lose suction-operated to magnetic nanoparticle.
Further, in the utility model, the contact surface in heating tank and described permanent magnet are in the different arrays into microwell plate, and both do not collide.
Further, in the utility model, the Control timing sequence of magnetic field, heating and vibrating function has following 6 kinds: 1) without magnetic field, without heating, friction; 2) without magnetic field, have heating, have vibration; 3) magnetic field is had, without heating, friction; 4) heat without magnetic field, nothing, have vibration; 5) magnetic field is had, without heating, friction; 6) without magnetic field, have heating, friction.
As shown in Fig. 1 to Fig. 5, a kind of heating vibration magnetic separating device of the present utility model comprises base 101, described base 101 is irregular grooves, centre is provided with three holddown grooves, fixing linear electric motors 202, electromagnetism load push-pull 102 and vibrating motor 201, middle both sides are the cuboid of projection, place two pieces of fixed heads 103.Corner Strapped 4 elasticity pillars 104 of described fixed head 103, described elasticity pillar 104 is cylinder, and in vertically placing in holddown groove, the infringement to instrument during in order to prevent from vibrating, plays cushioning effect.The top of elasticity pillar 104 is provided with holddown groove, for fixing and supportive body frame 105.Described ball 106 is fixed on described fixed terminal 107, and described fixed terminal 107 is positioned at the both sides, top of main frame 105, and the built-in described ball 106 of described fixed terminal 107, for fixing microwell plate, makes microwell plate be in same position all the time.Described fixed terminal 107 lays respectively at two minor faces of microwell plate, thus does not affect the crawl of mechanical gripper to microwell plate.Described main frame 105 center lower end connects an eccentric hammer 301, eccentric hammer 301 is connected with vibrating motor 201, vibrating motor 201 is energized, and to drive main frame 105 to realize of reciprocating vibration for rear drive eccentric hammer 301, thus drive the vibration being fixed on fixed terminal 107 place, main frame 105 top microwell plate.Described leading screw 203 one end is fixed on linear electric motors 202, in vertically placing, described leading screw connects magnet carrier 204, magnet carrier 204 stretches out the support of a cuboid, support is provided with 4 magnetic patch holes, place 4 block permanent magnets 205, permanent magnet 205 is in cuboid, parallel placement between permanent magnet 205, microwell plate common on the market divides according to different models 6 holes, 12 holes, 24 holes, 48 holes, 96 holes, 384 holes and 1536 holes etc., hole on microwell plate is according to array ordered arrangement, tubular container is provided with in each hole, namely pipe array is formed, certain intervals is there is between the pipe array of adjacent two row, identical interval between the column and the column, direction is also parallel each other, therefore, each permanent magnet 205 is all stuck in two pipe array spacings, realize the magnetic-particle in the tubular container of both sides in the reunion of sidewall and dispersion.Described electromagnetism load push-pull 102 is fixed a gripper shoe 206, in gripper shoe 206,2 support columns 208 are fixed in both sides, 2 support columns 208 are positioned at parallel placement on same level line, described spring 207 is positioned at the middle lower end of gripper shoe 206, be in the middle of two support columns 208, heating tank 108 is fixed on support column 208 top.Described heating tank 108 is irregular cuboids, is smooth heating surface 501 outside its both sides, places a heating film 109 respectively; Heating tank 108 has eight contact surfaces 502, the every a line in one_to_one corresponding microwell plate array, and contact surface 502 is structures of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, and realization is seamless applying with hole wall.Heating tank 108 top is the via hole 503 of adapt trickle orifice plate, makes microwell plate can put into heating tank 108 and fits with contact surface 502; Aperture on the corresponding microwell plate of aperture 504 on heating tank 108 top, can accelerate heat transfer.
For the nucleic acid extraction process based on Magneto separate, the concrete course of work of this device is as follows:
1, work is initial, is stuck in by microwell plate between two balls 106, places stable, the solution that the sample filling nucleic acid extraction in microwell plate mixes with lysate.This timer is in init state, and magnet carrier 204 and gripper shoe 206 are all positioned at the minimum point of respective vertical direction.The magnetic field of permanent magnet 205 does not affect the solution in microwell plate, and heating tank 108 departs from the tube wall in microwell plate array, and electromagnetism load push-pull 102, vibrating motor 201 and linear electric motors 202 are all in off working state.
2, timed incubations.Be energized to electromagnetism load push-pull 102, produce magnetic field, drive gripper shoe 206 to move upward, make heating tank 108 and microwell plate hole wall pastes with, be energized then to the heating film 109 of both sides, utilize metal heat-conducting principle to realize the temperature conduction of heating tank 108, realize the heating function of microwell plate.After certain hour, the power-off of electromagnetism load push-pull, now magnetic field dissipate, drive gripper shoe to move downward about 3mm, heating tank is separated with microwell plate, and the power-off of heating film 109, closes heating function.
3, vibration mixing.Experimentally demand selects whether open vibrating function.While heating, vibrating motor 201 can be opened, drive eccentric hammer 301 to drive main frame 105 to vibrate, thus realize the mixing of solution in microwell plate.Vibrating motor is closed while closing heating.
4, Magneto separate.The magnetic particle solution of the sample specific bond with nucleic acid extraction is added in microwell plate, opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward, permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, the material to be separated that magnetic-particle now in solution carries combination is adsorbed to sidewall, achieve sidewall to reunite, remaining liq can siphon away by modes such as pipettors, can being separated of realize target material and non-targeted material.
5, magnetic nanoparticle is discharged.Linear electric motors 202 antiport drives leading screw 203 to drive magnet carrier 204 to move downward, and make permanent magnet 205 rest in microwell plate bottom part down, now the magnetic of permanent magnet 205 loses suction-operated to the solution in microwell plate.
6, vibration mixing, adds cleaning fluid in microwell plate, opens vibrating motor 201, drives eccentric hammer 301 to drive main frame 105 to vibrate, realizes the mixing again of solution of magnetic nanoparticles in microwell plate.Close vibrating motor.
7, complete cleaning after repeating step 4-6 many times, eluent is added in microwell plate.
8, Magneto separate.Opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward, permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, the material to be separated that magnetic-particle now in solution carries combination is adsorbed to sidewall, achieve sidewall to reunite, remaining liq shifts by the modes such as available pipettor, and the liquid migrated out is the material of extraction.
The conversion of above-mentioned several duty can be realized by control system, attentional manipulation sequential, in conjunction with liquid-transfering device, the experiment of automatic biological sample process can be realized.
The above is only preferred embodiment of the present utility model; be noted that for those skilled in the art; under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
As shown in Fig. 1 to Fig. 5, a kind of heating vibration magnetic separating device of the present utility model comprises base 101, described base 101 is irregular grooves, centre is provided with three holddown grooves, fixing linear electric motors 202, electromagnetism load push-pull 102 and vibrating motor 201, middle both sides are the cuboid of projection, place two pieces of fixed heads 103.Corner Strapped 4 elasticity pillars 104 of described fixed head 103, described elasticity pillar 104 is cylinder, and in vertically placing in holddown groove, the infringement to instrument during in order to prevent from vibrating, plays cushioning effect.The top of elasticity pillar 104 is provided with holddown groove, for fixing and supportive body frame 105.Described ball 106 is fixed on described fixed terminal 107, and described fixed terminal 107 is positioned at the both sides, top of main frame 105, and the built-in described ball 106 of described fixed terminal 107, for fixing microwell plate, makes microwell plate be in same position all the time.Described fixed terminal 107 lays respectively at two minor faces of microwell plate, thus does not affect the crawl of mechanical gripper to microwell plate.Described main frame 105 center lower end connects an eccentric hammer 301, eccentric hammer 301 is connected with vibrating motor 201, vibrating motor 201 is energized, and to drive main frame 105 to realize of reciprocating vibration for rear drive eccentric hammer 301, thus drive the vibration being fixed on fixed terminal 107 place, main frame 105 top microwell plate.Described leading screw 203 one end is fixed on linear electric motors 202, in vertically placing, described leading screw connects magnet carrier 204, magnet carrier 204 stretches out the support of a cuboid, support is provided with 4 magnetic patch holes, place 4 block permanent magnets 205, permanent magnet 205 is in cuboid, parallel placement between permanent magnet 205, microwell plate common on the market divides according to different models 6 holes, 12 holes, 24 holes, 48 holes, 96 holes, 384 holes and 1536 holes etc., hole on microwell plate is according to array ordered arrangement, tubular container is provided with in each hole, namely pipe array is formed, certain intervals is there is between the pipe array of adjacent two row, identical interval between the column and the column, direction is also parallel each other, therefore, each permanent magnet 205 is all stuck in two pipe array spacings, realize the magnetic-particle in the tubular container of both sides in the reunion of sidewall and dispersion.Described electromagnetism load push-pull 102 is fixed a gripper shoe 206, in gripper shoe 206,2 support columns 208 are fixed in both sides, 2 support columns 208 are positioned at parallel placement on same level line, described spring 207 is positioned at the middle lower end of gripper shoe 206, be in the middle of two support columns 208, heating tank 108 is fixed on support column 208 top.Described heating tank 108 is irregular cuboids, is smooth heating surface 501 outside its both sides, places a heating film 109 respectively; Heating tank 108 has eight contact surfaces 502, the every a line in one_to_one corresponding microwell plate array, and contact surface 502 is structures of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, and realization is seamless applying with hole wall.Heating tank 108 top is the via hole 503 of adapt trickle orifice plate, makes microwell plate can put into heating tank 108 and fits with contact surface 502; Aperture on the corresponding microwell plate of aperture 504 on heating tank 108 top, can accelerate heat transfer.
For the nucleic acid extraction process based on Magneto separate, the concrete course of work of this device is as follows:
1, work is initial, is stuck in by microwell plate between two balls 106, places stable, the solution that the sample filling nucleic acid extraction in microwell plate mixes with lysate.This timer is in init state, and magnet carrier 204 and gripper shoe 206 are all positioned at the minimum point of respective vertical direction.The magnetic field of permanent magnet 205 does not affect the solution in microwell plate, and heating tank 108 departs from the tube wall in microwell plate array, and electromagnetism load push-pull 102, vibrating motor 201 and linear electric motors 202 are all in off working state.
2, timed incubations.Be energized to electromagnetism load push-pull 102, produce magnetic field, drive gripper shoe 206 to move upward, make heating tank 108 and microwell plate hole wall pastes with, be energized then to the heating film 109 of both sides, utilize metal heat-conducting principle to realize the temperature conduction of heating tank 108, realize the heating function of microwell plate.After certain hour, the power-off of electromagnetism load push-pull, now magnetic field dissipate, drive gripper shoe to move downward about 3mm, heating tank is separated with microwell plate, and the power-off of heating film 109, closes heating function.
3, vibration mixing.Experimentally demand selects whether open vibrating function.While heating, vibrating motor 201 can be opened, drive eccentric hammer 301 to drive main frame 105 to vibrate, thus realize the mixing of solution in microwell plate.Vibrating motor is closed while closing heating.
4, Magneto separate.The magnetic particle solution of the sample specific bond with nucleic acid extraction is added in microwell plate, opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward, permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, the material to be separated that magnetic-particle now in solution carries combination is adsorbed to sidewall, achieve sidewall to reunite, remaining liq can siphon away by modes such as pipettors, can being separated of realize target material and non-targeted material.
5, magnetic nanoparticle is discharged.Linear electric motors 202 antiport drives leading screw 203 to drive magnet carrier 204 to move downward, and make permanent magnet 205 rest in microwell plate bottom part down, now the magnetic of permanent magnet 205 loses suction-operated to the solution in microwell plate.
6, vibration mixing, adds cleaning fluid in microwell plate, opens vibrating motor 201, drives eccentric hammer 301 to drive main frame 105 to vibrate, realizes the mixing again of solution of magnetic nanoparticles in microwell plate.Close vibrating motor.
7, complete cleaning after repeating step 4-6 many times, eluent is added in microwell plate.
8, Magneto separate.Opening linear electric motors 202 drives leading screw 203 to drive magnet carrier 204 to move upward, permanent magnet 205 is made to rest in microwell plate pore sidewall, the solution in two-sided array can be adsorbed simultaneously, the material to be separated that magnetic-particle now in solution carries combination is adsorbed to sidewall, achieve sidewall to reunite, remaining liq shifts by the modes such as available pipettor, and the liquid migrated out is the material of extraction.
The conversion of above-mentioned several duty can be realized by control system, attentional manipulation sequential, in conjunction with liquid-transfering device, the experiment of automatic biological sample process can be realized.
The above is only preferred embodiment of the present utility model; be noted that for those skilled in the art; under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
The above is only preferred embodiment of the present utility model; be noted that for those skilled in the art; under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (2)
1. heat an oscillating magnetic flux separator, it is characterized in that: this device comprises three primary modules, i.e. Magneto separate module, heating module and vibration module, and auxiliary body;
Magneto separate module comprises linear electric motors (202), leading screw (203), magnet carrier (204), permanent magnet (205); Heating module comprises electromagnetism load push-pull (102), gripper shoe (206), spring (207), support column (208), heating tank (108), heating film (109); Vibration module involving vibrations motor (201), eccentric hammer (301); Auxiliary body comprises base (101), fixed head (103), 4 elasticity pillars (104), main frame (105);
Described base (101) is in groove-like, centre is provided with three holddown grooves, be respectively used to fixing linear electric motors (202), electromagnetism load push-pull (102) and vibrating motor (201), middle two lateral processes place two pieces of fixed heads (103); Described fixed head (103) corner is provided with 4 elasticity pillar holddown grooves, and described elasticity pillar (104) is placed in vertical in holddown groove;
Described main frame (105) is placed on 4 elasticity pillars (104), described main frame (105) center lower end connects an eccentric hammer (301), and eccentric hammer (301) is connected with vibrating motor (201);
Minor face both sides, described main frame (105) top are two fixed terminals (107), and described fixed terminal (107) built-in ball (106), for fixing microwell plate, makes microwell plate be in same position all the time; Described fixed terminal (107) lays respectively at two minor faces of microwell plate, thus does not affect the crawl of mechanical arm to microwell plate;
Described leading screw (203) one end is fixed on linear electric motors (202), in vertically placing, described leading screw connects magnet carrier (204), magnet carrier (204) stretches out the support of a cuboid, support is provided with 4 magnetic patch holes, place 4 pieces of permanent magnets (205), permanent magnet (205) is in cuboid, parallel placement between permanent magnet (205), one between pipe array adjacent on microwell plate in the corresponding 4 pieces of permanent magnets (205) of difference, realize the reunion of magnetic nanoparticle at sidewall;
Described electromagnetism load push-pull (102) is fixed a gripper shoe (206), 2 support columns (208) are fixed in the upper both sides of gripper shoe (206), 2 support columns (208) are positioned at parallel placement on same level line, described spring (207) is positioned at lower end in the middle of gripper shoe (206), be in the middle of two support columns (208), heating tank (108) is fixed on support column (208) top;
Described heating tank (108) is an irregular cuboid, is smooth heating surface (501) outside two long limit, places heating film (109) respectively; Heating tank has 8 contact surfaces (502), the every a line in one_to_one corresponding microwell plate array, and contact surface (502) is the structure of multiple taper surface, and the tapering of taper surface mates with the tapering of tubular container, and realization is seamless applying with hole wall; Heating tank top is the via hole (503) of adapt trickle orifice plate, makes microwell plate can put into heating tank and fits with contact surface (502); Aperture on aperture (504) the corresponding microwell plate on heating tank top, can accelerate heat transfer.
2. heating oscillating magnetic flux separator according to claim 1, it is characterized in that: the contact surface (502) in described heating tank (108) and described permanent magnet (205) are in the different arrays into microwell plate, and both do not collide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201420401915.9U CN204018008U (en) | 2014-07-18 | 2014-07-18 | A kind of heating oscillating magnetic flux separator |
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| CN201420401915.9U CN204018008U (en) | 2014-07-18 | 2014-07-18 | A kind of heating oscillating magnetic flux separator |
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| CN204018008U true CN204018008U (en) | 2014-12-17 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104117429A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Heating, vibrating and magnetic separating device |
-
2014
- 2014-07-18 CN CN201420401915.9U patent/CN204018008U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104117429A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Heating, vibrating and magnetic separating device |
| CN104117429B (en) * | 2014-07-18 | 2016-08-17 | 东南大学 | A kind of heating oscillating magnetic flux separator |
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