CN216513879U - Culture and collection device for high-pressure microbial gas production - Google Patents

Abstract

The utility model discloses a high-pressure microbial aerogenesis culturing and collecting device.A tank body for culturing and collecting gas is arranged in a constant temperature control box, and the tank body is a detachable tank cover, an inner culturing chamber and a piston chamber; the tank cover is provided with a temperature detection hole, an exhaust port, a pressure sensor connecting port and a sample inlet; the temperature detection hole and the pressure sensor connecting port are respectively connected with a corresponding sensor and a display on the outer constant temperature control box; the exhaust port is respectively connected with the gas collecting bag and the gas drainage bottle through a tee joint; the culture chamber can adjust the liquid filling proportion according to the requirement; the bottom of the piston chamber is provided with a control valve and is connected with an external constant flow pump, and the pressure in the tank can be controlled according to the requirement. The device has reasonable structural design, can effectively collect the gas generated by the microorganisms, performs qualitative and quantitative analysis on the gas, can realize the gas generation condition of the microorganisms under the high-pressure sterilization of the tank body, the simulated pressure environment or the approximate anaerobic environment, and has important significance for researching the gas generation of the microorganisms in the oil reservoir and the qualitative and quantitative analysis.

Description

Culture and collection device for high-pressure microbial gas production

Technical Field

The utility model belongs to the field of microbial oil production, and particularly relates to a high-pressure microbial aerogenesis culture and collection device.

Background

The traditional microorganism culture and gas collection method is a ' Du ' tubule method ', the method can only be used for judging whether the microorganism produces gas, but has some defects as a microorganism culture and gas production collection device: (1) after the culture medium in the test tube is sterilized, the gas in the Du's tubule is inexhaustible and has small bubbles, and the residual small bubbles can interfere the judgment of the experimental result; (2) when the gas production capacity of certain microorganism is weak, the gas can not be collected by the Du's tubule; (3) the method is characterized in that a part of gas collected by the Du's tubule is limited and is not easy to take out for qualitative and quantitative analysis, and the' Du's tubule method' cannot be adopted when the quantity and the gas components of the gas produced by a certain microorganism are required to be qualitatively and quantitatively analyzed.

Disclosure of Invention

The utility model aims to overcome the defects of the prior art, provides a high-pressure microbial aerogenesis culture and collection device, solves the problems of microbial culture temperature detection, realizes culture under high pressure, can realize nitrogen deoxidization to enable the anaerobic condition to be close to the anaerobic condition, completely monitors and collects the gas generated by the microbes, and realizes qualitative and quantitative analysis on the gas.

The utility model discloses a high-pressure microorganism culturing and collecting device, which comprises a constant temperature control box and a tank body, wherein the constant temperature control box consists of a control chamber and a working chamber, the tank body is arranged in the working chamber, and the tank body is sequentially divided into a detachable tank cover at the upper part, an inner culturing chamber at the middle part, a piston chamber at the lower part and a tank body base from top to bottom; a temperature detection hole, an exhaust port, a pressure sensor connecting port and a sample inlet are formed in the tank cover; the temperature detection hole and the pressure sensor connecting port are respectively provided with a temperature sensor and a pressure sensor, the temperature sensor is connected with a temperature display panel in the control room, and the pressure sensor is connected with a pressure display panel in the control room; the exhaust port is respectively connected with the gas collecting bag and the gas drainage bottle through a tee joint; the bottom of the lower piston chamber is provided with a valve V and is connected with a constant flow pump.

As a further improvement of the utility model, a piston is arranged between the inner culture chamber and the lower piston chamber for isolation.

As a further improvement of the utility model, a valve IV is arranged on the exhaust port and is connected with a tee joint through a pipeline A, the other two outlets of the tee joint are respectively connected with a gas collecting bag and a gas exhaust bottle through a pipeline A1 and a pipeline A2, a valve I is arranged on a pipeline A1, and a valve II is arranged on a pipeline A2; the gas drainage bottle is connected with the scale test tube through a pipeline D.

As a further improvement of the utility model, the sample inlet is connected with a nitrogen cylinder through a pipeline C, and a valve III is arranged on the pipeline C.

The culture and collection device for high-pressure microbial aerogenes has reasonable structural design, and the base can be moved, so that the high-pressure sterilization of the whole tank body can be realized; nitrogen can be connected through a sample inlet to remove oxygen so that the inside of the tank is similar to an anaerobic environment; the temperature and pressure sensor and the display panel are connected through the corresponding interfaces on the tank, so that the whole-process monitoring of the temperature and the pressure can be realized; through the piston design of the tank body, the culture under the high-pressure environment can be simulated. The device provided by the utility model can realize constant-temperature and high-pressure culture under a target condition, can monitor and effectively collect the gas production process of the microorganisms, and has important significance for researching oil reservoir microorganism gas production and more accurate qualitative and quantitative analysis.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Detailed Description

The utility model relates to a high-pressure microorganism culturing and collecting device, which comprises a constant temperature control box 1 and tank bodies 2, wherein the constant temperature control box 1 consists of a control chamber 22 and a working chamber 23, a plurality of tank bodies 2 can be placed in the working chamber 23 according to requirements, and the tank bodies 2 are stainless steel tank bodies (withstand pressure of 35 MPa) and are sequentially divided into a detachable tank cover 3 at the upper part, an inner culturing chamber 4 at the middle part, a piston chamber 5 at the lower part and a tank body base 19 from top to bottom; wherein, a piston 27 is provided between the inner culture chamber 4 and the lower piston chamber 5 for isolation. A valve V20 is arranged at the bottom of the piston chamber 5 and is connected with an external constant flow pump 21 through a pipeline B. The liquid filling proportion of a middle culture chamber of the device can be adjusted according to the requirement; the bottom of the lower piston chamber is provided with a control valve and is connected with an external constant flow pump, and the pressure in the tank can be controlled according to the requirement.

A temperature detection hole 6, an exhaust port 7, a pressure sensor connecting port 8 and a sample inlet 9 are arranged on the tank cover 3; the sample inlet 9 is connected with a nitrogen cylinder 29 through a pipeline C, and a valve III 30 is arranged on the pipeline C. The temperature detection hole 6 and the pressure sensor connecting port 8 are respectively provided with a temperature sensor 10 and a pressure sensor 11, the temperature sensor 10 is connected with a temperature display panel 26 in the control chamber 22, and the pressure sensor 11 is connected with a pressure display panel 25 in the control chamber 22; a valve IV 15 is arranged on the exhaust port 7 and is connected with a tee joint 12 through a pipeline A, the other two outlets of the tee joint 12 are respectively connected with a gas collecting bag 14 and a gas drainage bottle 17 through a pipeline A1 and a pipeline A2, a valve I13 is arranged on a pipeline A1, and a valve II 16 is arranged on a pipeline A2; the gas drainage bottle 17 is connected with a graduated test tube 18 through a pipeline D.

The stainless steel tank body can move integrally to perform autoclaving, the tank cover 3 needs to be opened, a target culture solution needs to be injected, the temperature detection hole 6, the exhaust port 7, the pressure sensor connecting port 8 and the sample inlet 9 which are arranged on the tank cover 3 are sealed, and the valve IV 15 at the exhaust port 7 is in a closed state.

The operation process of the stainless steel tank after sterilization is as follows: the tank body 2 is placed in a working chamber 23 of the thermostatic control box 1 with an air blowing heating device, the target temperature of the thermostatic control box 1 is set through a temperature control panel 24, and the temperature of the thermostatic control box 1 is controlled by starting the air blowing heating device. Connecting the temperature detection hole 6 on the tank cover 3 with the temperature sensor 10 and the temperature display panel 26; the pressure sensor connection port 8 is connected to the pressure sensor 11 and the pressure display panel 25. The valve IV 15 at the air outlet is connected with the tee joint 12 through a pipeline A, the pipeline A1 is provided with a valve I13, the pipeline A2 is provided with a valve II 16 which is connected with the gas collection bag 14 and the gas drainage bottle 17, and the gas drainage bottle 17 is connected with the graduated test tube 18 through a pipeline D.

After the process, sterile inoculation is carried out through the sample inlet 9, and then the sterile inoculation can be carried out through connecting a pipeline C of the sample inlet 9 to be connected with a nitrogen bottle 29 according to the requirement, and a valve III 30 is arranged in the middle to remove oxygen.

In the pressure control process of the piston chamber 5, a valve v 20 is arranged at the bottom of the piston chamber 5 and connected with an external constant flow pump 21 through a pipeline B, and the external constant flow pump 21 injects liquid 28 pure water or kerosene into the piston chamber 5 to adjust the pressure to a target pressure, and the valve v 20 is closed.

During the operation process of the gas collecting bag 14 or the gas drainage bottle 17, if gas collection is carried out according to the experimental requirements, the valve II 16 is closed, the valve IV 15 is opened, and the valve I13 is connected with the gas collecting bag. If the gas volume measurement is needed, the valve I13, the valve IV 15 and the valve II 16 are required to be closed and connected with a gas drainage bottle 17 and a graduated test tube 18.

Claims (4)

1. A high-pressure microbial aerogenesis culturing and collecting device comprises a constant-temperature control box (1) and a tank body (2), and is characterized in that the constant-temperature control box (1) consists of a control chamber (22) and a working chamber (23), the tank body (2) is arranged in the working chamber (23), and the tank body (2) is sequentially divided into a detachable tank cover (3) at the upper part, an inner culturing chamber (4) at the middle part, a piston chamber (5) at the lower part and a tank body base (19) from top to bottom; a temperature detection hole (6), an exhaust port (7), a pressure sensor connecting port (8) and a sample inlet (9) are arranged on the tank cover (3); a temperature sensor (10) and a pressure sensor (11) are respectively arranged on the temperature detection hole (6) and the pressure sensor connecting port (8), the temperature sensor (10) is connected with a temperature display panel (26) in the control chamber (22), and the pressure sensor (11) is connected with a pressure display panel (25) in the control chamber (22); the exhaust port (7) is respectively connected with a gas collecting bag (14) and a gas drainage bottle (17) through a tee joint (12); the bottom of the lower piston chamber (5) is provided with a valve V (20) and is connected with a constant flow pump (21).

2. A high pressure microbial gas production culture and collection device according to claim 1 wherein a piston (27) is provided between the inner culture chamber (4) and the lower piston chamber (5) for isolation.

3. The high-pressure microbial aerogenesis culturing and collecting device according to claim 1, wherein a valve IV (15) is arranged on the exhaust port (7) and is connected with a tee joint (12) through a pipeline A, the other two outlets of the tee joint (12) are respectively connected with a gas collecting bag (14) and a gas exhaust bottle (17) through a pipeline A1 and a pipeline A2, a valve I (13) is arranged on a pipeline A1, and a valve II (16) is arranged on a pipeline A2; the gas drainage bottle (17) is connected with a graduated test tube (18) through a pipeline D.

4. The high-pressure microbial aerogenesis culture and collection device according to claim 1, wherein the sample inlet (9) is connected with a nitrogen gas bottle (29) through a pipeline C, and a valve III (30) is arranged on the pipeline C.

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