CN218491747U - Multi-channel rumen fluid in-vitro fermentation gas production measuring device - Google Patents
Multi-channel rumen fluid in-vitro fermentation gas production measuring device Download PDFInfo
- Publication number
- CN218491747U CN218491747U CN202222441516.2U CN202222441516U CN218491747U CN 218491747 U CN218491747 U CN 218491747U CN 202222441516 U CN202222441516 U CN 202222441516U CN 218491747 U CN218491747 U CN 218491747U
- Authority
- CN
- China
- Prior art keywords
- fermentation
- gas
- channel
- rumen
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a multichannel rumen fluid external fermentation gas production survey device relates to ruminant rumen external fermentation technical field, including a plurality of fermentation tanks, the fermentation tank passes through the intake pipe and is connected with inlet channel in proper order, the fermentation tank passes through the blast pipe and is connected with exhaust passage in proper order, set up the admission valve in the intake pipe, be provided with discharge valve on the blast pipe, inlet channel and N2 gas cylinder are connected, be provided with gas flowmeter between N2 gas cylinder and the head end fermentation cylinder, the blast pipe is connected with multichannel gas analyzer. Through the utility model discloses a set up, produce gas when providing a measurable multiunit rumen liquid external fermentation, realize the real time monitoring to each fermentation parameter in the fermentation environment to carry out accurate measurement to fermentation product like gas production volume etc. and be used for rumen microorganism metabolism and assess the external fermentation of multichannel rumen liquid and produce gas measuring device of research in the aspect of daily ration nutritive value etc..
Description
Technical Field
The utility model relates to a ruminant rumen external fermentation technical field especially relates to a gas survey device is produced in external fermentation of multichannel rumen liquid.
Background
Rumen is used as a microbial fermentation tank, provides more than 70% of body energy for ruminants, and is the key point of nutrition research of ruminants. The technology for simulating rumen fermentation in vitro has the advantages of short test period, high repetition number, no limitation by in-vivo animal tests and the like, is an important means for evaluating rumen function and forage quality, and is widely applied in the fields of rumen physiology, ruminant animal nutrition, feed nutritive value evaluation and the like.
The rumen fluid in-vitro fermentation gas production measuring device in the prior art can only detect the fermentation parameters of rumen fluid in a single tank body, and the efficiency is low.
Therefore the utility model provides a but produce gas when the external fermentation of survey multiunit rumen liquid realizes the real time monitoring to each fermentation parameter in the fermentation environment to fermentation product like producing gas volume etc. carry out accurate measurement, a multichannel rumen liquid external fermentation gas production survey device that is used for aspects such as rumen microorganism metabolism and appraises daily ration nutritive value to study.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a multi-channel rumen fluid in-vitro fermentation gas production measuring device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a gas survey device is produced in external fermentation of multichannel rumen fluid, includes a plurality of fermentation tanks, the fermentation tank passes through the intake pipe and sets gradually on inlet channel, the fermentation tank passes through the blast pipe and is connected with exhaust passage in proper order, set up the admission valve in the intake pipe, be provided with discharge valve on the blast pipe, inlet channel is connected with the N2 gas cylinder, be provided with gas flowmeter between N2 gas cylinder and the head end fermentation cylinder, the blast pipe is connected with multichannel gas analyzer.
Furthermore, the multi-channel gas analyzer is connected with a computer through a signal acquisition controller, and a CO2 sensor, a CH4 sensor and an H2 sensor are arranged in the multi-channel gas analyzer.
Further, the fermentation cylinder includes the end cover and a jar body, end cover and jar body threaded connection, both cooperation position departments are provided with the silica gel sealing washer, are provided with intake pipe and blast pipe above the end cover, and jar internal rumen liquid, fodder or feed additive that sets up.
Furthermore, the tank body is made of waterproof and light-tight materials.
Furthermore, a filter screen is arranged on the end cover, and the filter screen is arranged outside the part of the exhaust pipe extending into the tank body.
Furthermore, a pressure reducing valve is arranged between the N2 gas cylinder and the gas flowmeter.
Furthermore, a manual valve is arranged on the air inlet channel and is arranged in front of the head-end fermentation tank.
Advantageous effects
Compared with the prior art, the beneficial effects of the utility model reside in that: through the utility model discloses a set up, produce gas when providing a measurable multiunit rumen liquid external fermentation, realize the real time monitoring to each fermentation parameter in the fermentation environment to carry out accurate measurement to the fermentation product like gas production volume etc. for rumen microorganism metabolism and the multi-channel rumen liquid external fermentation gas production survey device who assesses aspects such as daily ration nutritive value and study.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the principles of the invention.
FIG. 1 is a schematic view of the overall structure of a gas production measuring apparatus;
FIG. 2 is a schematic view of the structure of a fermenter.
In the figure: 1. an N2 gas cylinder; 2. a pressure reducing valve; 3. a gas flow meter; 4. an air inlet pipe; 5. a fermentation tank; 6. a check valve; 7. a manual valve; 8. an exhaust pipe; 9. a multi-channel gas analyzer; 10. a signal acquisition control instrument; 11. a computer.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Referring to fig. 1-2, a multi-channel rumen fluid in-vitro fermentation and gas production measuring device comprises a plurality of fermentation tanks, preferably 6 fermentation tanks,
the gas inlet channel is sequentially provided with an N2 gas cylinder 1, a pressure reducing valve 2, a gas flowmeter 3, a plurality of groups of gas inlet pipes and a check valve 6 from head to tail, and a plurality of groups of fermentation tanks are connected with the gas inlet channel through the gas inlet pipes 4; in use, the air inlet pipe 4 is inserted below the liquid in the fermentation tank.
The exhaust channel is sequentially provided with a manual valve 7, a plurality of groups of exhaust pipes and a multi-channel gas analyzer 9 from head to tail, and the plurality of groups of fermentation tanks are connected with the exhaust channel through the exhaust pipes;
wherein, the air inlet pipe is provided with an air inlet valve, and the exhaust pipe 5 is provided with an exhaust valve;
specifically, a multi-channel gas analyzer 9 is connected with a computer 11 through a signal acquisition controller 10, and a CO2 sensor, a CH4 sensor and an H2 sensor are arranged in the multi-channel gas analyzer. After the fermentation gas is produced, the gas is supplied to a gas analyzer for analyzing the gas concentration of CO2, CH4 and H2 or for a feed digestibility test.
In his preferred embodiment, the fermentation cylinder includes the end cover and jar body, and end cover and jar body threaded connection are provided with the silica gel sealing washer in the position of both cooperations, are provided with intake pipe and blast pipe above the end cover, jar internal rumen liquid, fodder or feed additive of setting.
Specifically, the tank body is made of a waterproof and light-tight material. The fermentation tank can be made of white steel or a water cup. If the cup is used, the outer surface of the cup body needs to be coated with black waterproof paint.
Specifically, be provided with the filter screen on the end cover, the filter screen panel is located outside the blast pipe, has installed a filter screen (white steel material) on this blast pipe in the fermentation cylinder, prevents that the zymotic fluid from foaming the back, takes debris to the gas circuit in.
Preferably, the fermentation tank is a 500mL-800mL fermentation tank, the end cover is in threaded connection with the tank body, a silica gel sealing ring is arranged at the matching position of the end cover and the tank body, an air inlet pipe for supplying nitrogen is arranged on the upper surface of the end cover, the fermentation tank is also provided with an exhaust pipe, and rumen fluid, feed or feed additives and other substances are filled in the tank.
The working principle of the 6-channel rumen fluid in-vitro fermentation gas production determination device is as follows:
because anaerobic fermentation is adopted, nitrogen is used for maintaining anaerobic fermentation when air is fed into each fermentation tank, normally open electromagnetic valves are respectively arranged on the air inlet pipe and the air outlet pipe, a pressure reducing valve on a nitrogen bottle is opened before measurement is started, and then a micro pressure reducing valve is adjusted to control the gas flow of the nitrogen to be 400ml. At the moment, nitrogen can not enter each fermentation tank and is emptied from the check valve.
When the gas analyzer is started, the manual gas valve is firstly opened, so that the gas analyzer is firstly preheated for about 30-60 min.
Then placing 1-6 fermentation tanks on the water bath (the temperature of the water bath of the shaking table is 39 ℃), and then using the gas path of each fermentation tank
The silicone tube was connected, then the computer program was started, the gas collection time for each fermentor was set to 1-5min, and the test was started (then the manual gas valve was closed).
Gas cycle collection sequence for each fermentor:
when gas in a fermentation tank No. 1 (a first-end fermentation tank) is collected, an air inlet valve and an air outlet valve (both are electromagnetic valves) of the fermentation tank No. 1 are automatically opened, other electromagnetic valves are closed, and then redundant gas in the fermentation tank No. 1 is emptied;
when the gas in the fermentation tank No. 2 is measured, an air inlet valve and an air outlet valve (both are electromagnetic valves) of the fermentation tank No. 2 are automatically opened, other electromagnetic valves are closed, and then the redundant gas in the fermentation tank No. 2 is emptied;
when the gas in the fermentation tank No. 3 is measured, the gas inlet valve and the gas outlet valve (both are electromagnetic valves) of the fermentation tank No. 3 are automatically opened, other electromagnetic valves are closed, and then the redundant gas in the fermentation tank No. 3 is emptied;
when the gas in the fermentation tank No. 4 is measured, an air inlet valve and an air outlet valve (both are electromagnetic valves) of the fermentation tank No. 4 are automatically opened, other electromagnetic valves are closed, and then the redundant gas in the fermentation tank No. 4 is emptied;
when the gas in the fermentation tank No. 5 is measured, the air inlet valve and the air outlet valve (both are electromagnetic valves) of the fermentation tank No. 5 are automatically opened, other electromagnetic valves are closed, and then the redundant gas in the fermentation tank No. 5 is emptied;
when measuring the gas in the No. 6 fermentation tank, the gas inlet valve and the gas outlet valve (both are electromagnetic valves) of the No. 6 fermentation tank are automatically opened, other electromagnetic valves are closed, and then the redundant gas in the No. 5 fermentation tank is emptied; and (4) measuring in a reciprocating cycle.
Wherein, the gas production formula is:
1.CO2L/min/g = gas flow (L/min). Times.CO 2%
2.CH4L/min/g = gas flow (L/min). Times.CH 4%
H2L/min/g = gas flow (L/min). Times.H 2%
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (7)
1. The utility model provides a gas survey device is produced in external fermentation of multichannel rumen liquid which characterized in that, includes a plurality of fermentation tanks, the fermentation tank passes through the intake pipe and sets gradually on inlet channel, the fermentation tank passes through the blast pipe and is connected with exhaust passage in proper order, set up the admission valve in the intake pipe, be provided with discharge valve on the blast pipe, inlet channel and N2 gas cylinder connection, be provided with gas flowmeter between N2 gas cylinder and the head end fermentation cylinder, the blast pipe is connected with multichannel gas analyzer.
2. The apparatus for determining in-vitro fermentation and gas production of multi-channel rumen fluid according to claim 1, wherein the multi-channel gas analyzer is connected with a computer through a signal acquisition controller, and a CO2 sensor, a CH4 sensor and an H2 sensor are arranged in the multi-channel gas analyzer.
3. The apparatus for measuring the in vitro fermentation and gas production of rumen fluid according to claim 1, wherein the fermentation tank comprises an end cap and a tank body, the end cap is in threaded connection with the tank body, a silica gel sealing ring is arranged at the matching position of the end cap and the tank body, an air inlet pipe and an exhaust pipe are arranged on the end cap, and rumen fluid, feed or feed additive is arranged in the tank body.
4. The apparatus for measuring in vitro fermentation and gas production of multi-channel rumen fluid as claimed in claim 3, wherein said tank is made of waterproof and opaque material.
5. The apparatus of claim 3, wherein a filter screen is disposed on the end cap, and the filter screen is disposed outside the portion of the exhaust pipe extending into the tank.
6. The apparatus for measuring the in vitro fermentation and gas production of rumen fluid by multi-channel according to claim 1, wherein a pressure reducing valve is arranged between the N2 gas cylinder and the gas flowmeter.
7. The apparatus for measuring the in vitro fermentation and gas production of gastric rumen fluid according to claim 1, wherein the gas inlet channel is provided with a manual valve, and the manual valve is arranged in front of the head-end fermentation tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222441516.2U CN218491747U (en) | 2022-09-15 | 2022-09-15 | Multi-channel rumen fluid in-vitro fermentation gas production measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222441516.2U CN218491747U (en) | 2022-09-15 | 2022-09-15 | Multi-channel rumen fluid in-vitro fermentation gas production measuring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218491747U true CN218491747U (en) | 2023-02-17 |
Family
ID=85191151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222441516.2U Active CN218491747U (en) | 2022-09-15 | 2022-09-15 | Multi-channel rumen fluid in-vitro fermentation gas production measuring device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218491747U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117720994A (en) * | 2024-02-07 | 2024-03-19 | 湖南碧臣环境能源有限公司 | In-vitro digestion bionic device, control and degradation test method |
| CN117736840A (en) * | 2024-02-07 | 2024-03-22 | 湖南碧臣环境能源有限公司 | Rumen reticulum in-vitro fermentation reactor and control method |
-
2022
- 2022-09-15 CN CN202222441516.2U patent/CN218491747U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117720994A (en) * | 2024-02-07 | 2024-03-19 | 湖南碧臣环境能源有限公司 | In-vitro digestion bionic device, control and degradation test method |
| CN117736840A (en) * | 2024-02-07 | 2024-03-22 | 湖南碧臣环境能源有限公司 | Rumen reticulum in-vitro fermentation reactor and control method |
| CN117720994B (en) * | 2024-02-07 | 2024-05-17 | 湖南碧臣环境能源有限公司 | In-vitro digestion bionic device, control and degradation test method |
| CN117736840B (en) * | 2024-02-07 | 2024-05-17 | 湖南碧臣环境能源有限公司 | Rumen reticulum in-vitro fermentation reactor and control method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN218491747U (en) | Multi-channel rumen fluid in-vitro fermentation gas production measuring device | |
| CN201034999Y (en) | Soil respiration continuance measuring equipment | |
| CN201607352U (en) | Quantitative sampling device for liquid | |
| CN104498357B (en) | 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems | |
| CN110568139A (en) | Water quality detection device | |
| CN201348589Y (en) | High precision pressure resistant performance tester of aerosol tank | |
| CN206646115U (en) | A kind of fermentation tank for research and teaching | |
| CN201908651U (en) | Nesting type combined sealing can | |
| CN207468625U (en) | Homogenous anaerobic fermenting experiment device | |
| CN209784320U (en) | Indoor air formaldehyde detection device | |
| CN209247683U (en) | A kind of automatic control oxygen consumption rate detection device | |
| CN202152343U (en) | Artificial rumen in-vitro fermentation gas-producing device | |
| CN201034941Y (en) | Water quality test box | |
| CN204347025U (en) | 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems | |
| CN206020212U (en) | A kind of BOD online auto monitoring systems | |
| CN204807350U (en) | Novel sampling of ammonia nitrogen on -line monitoring instrument ration device | |
| CN203519627U (en) | Metering sampling device of online automatic water quality COD monitor | |
| CN208043451U (en) | A kind of land resources detection sampler | |
| CN211771359U (en) | Simple and easy cell oxygen deficiency culture apparatus | |
| CN204779580U (en) | Simple and easy artificial rumen blake bottle | |
| CN208833744U (en) | A kind of water monitoring device | |
| CN209102465U (en) | A kind of experiment fermentation gas collection and measuring device | |
| CN210894003U (en) | Instrument for measuring oxidation stability of lubricating oil and insulating oil | |
| CN220846129U (en) | Simple in-vitro fermentation device | |
| CN209167231U (en) | A kind of measurement sediments greenhouse gases generate the incubator of rate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |