CN204347025U - 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems - Google Patents
6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems Download PDFInfo
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- CN204347025U CN204347025U CN201520025784.3U CN201520025784U CN204347025U CN 204347025 U CN204347025 U CN 204347025U CN 201520025784 U CN201520025784 U CN 201520025784U CN 204347025 U CN204347025 U CN 204347025U
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Abstract
6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems belong to ruminant tumor gastric juice aerogenesis detection technique field, in the utility model, gas analyzer is connected with microcomputer through data acquisition control instrument, pipeline g porch is provided with hand valve o, and is communicated with gas analyzer entrance through 6 two-position three way magnetic valve entrances; Gas outlet m outlet is provided with hand valve p, and through 6 two-position three way magnetic valve outlets and gas analyzer outlet; Draft tube a inlet end is communicated with nitrogen cylinder, and is connected in series reduction valve, spinner-type flowmeter, draft tube b inlet end, hand valve b, and is communicated with pipeline a-f entrance; Draft tube b porch is provided with hand valve a, and order is communicated with gas outlet a-f; The entrance and exit of 6 fermentation tanks is parallel between pipeline g and gas outlet m through pipeline respectively, and 6 two-position three way magnetic valves control by microcomputer; The CO that the utility model produces when truly quantitatively can detect ruminant tumor gastric juice anaerobic fermentation fast online
2, CH
4amount, gas reclaiming rate more than 98%.
Description
Technical field
The utility model belongs to ruminant tumor gastric juice aerogenesis detection technique field, is specifically related to a kind of 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems.
Background technology
The test of ruminant live body lumen fermentation is research rumen microorganism nutrition and metabolism; The important means of evaluation nutritive value and nutrition regulation efficiency.But because its cycle is long, need that animal is many, environmental baseline is wayward, data stationarity is poor and high in cost of production factor, restriction further investigation.The forties in 20th century, the in-vitro simulated fermentation technique of ruminant tumor gastric juice starts to occur, the in-vitro simulated method of lumen fermentation conventional at present mainly comprises: two-step approach, In Vitro gas production method and artificial rumen continue dynamic fermentation etc.Menke etc. (1979) utilize In Vitro gas production method to carry out simulation rumen fermentation test, and it can only determine total gas production rate, and the method is to CO
2, CH
4mensuration need complete by gas chromatograph, can not on-line checkingi simulation rumen liquid anaerobic fermentation produce CO
2, CH
4amount.Again because repeatedly extracting gas in fermentation tank, causing gas loss and affecting test findings.And cud is a lasting dynamic anaerobic fermentation system, gas production rate and gas (carbon dioxide, methane etc.) proportion of composing are also dynamic.
Summary of the invention
The purpose of this utility model is the 6 passage rumen fluid In Vitro Fermentation aerogenesis pick-up unit systems providing a kind of energy high-speed simulation simulation on-line checkingi ruminant tumor gastric juice.
The utility model is by fermentation tank I A, fermentation tank II B, fermentation tank III C, fermentation tank IV D, fermentation tank V E, fermentation tank VI F, nitrogen cylinder G, reduction valve H, spinner-type flowmeter I, gas analyzer J, data acquisition control instrument K, microcomputer L, two-position three way magnetic valve I entrance 1, two-position three way magnetic valve II entrance 2, two-position three way magnetic valve III entrance 3, two-position three way magnetic valve IV entrance 4, two-position three way magnetic valve V entrance 5, two-position three way magnetic valve VI entrance 6, two-position three way magnetic valve I exports 1 ', two-position three way magnetic valve II exports 2 ', two-position three way magnetic valve III exports 3 ', two-position three way magnetic valve IV exports 4 ', two-position three way magnetic valve V exports 5 ', two-position three way magnetic valve VI exports 6 ', hand valve a7, hand valve b8, hand valve c9, hand valve d10, hand valve e11, hand valve f12, hand valve g13, hand valve h14, hand valve i 15, hand valve j 16, hand valve k17, hand valve l 18, hand valve m19, hand valve n20, hand valve o21, hand valve p22, gas outlet a23, gas outlet b24, gas outlet c25, gas outlet d26, gas outlet e27, gas outlet f28, gas outlet g29, gas outlet h30, gas outlet i31, gas outlet j32, gas outlet k33, gas outlet l34, gas outlet m35, gas outlet n36, gas outlet o37, gas outlet p38, gas outlet q39, gas outlet r40, gas outlet s41, draft tube a42, draft tube b43, pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49 and pipeline g50 forms, and wherein gas analyzer J is connected with microcomputer L through data acquisition control instrument K.
Pipeline g50 porch is provided with hand valve o21, and is communicated with the entrance of gas analyzer J through two-position three way magnetic valve I entrance 1, two-position three way magnetic valve II entrance 2, two-position three way magnetic valve III entrance 3, two-position three way magnetic valve IV entrance 4, two-position three way magnetic valve V entrance 5, two-position three way magnetic valve VI entrance 6.
Gas outlet m35 exit is provided with hand valve p22, and export 1 ' through two-position three way magnetic valve I, two-position three way magnetic valve II exports 2 ', two-position three way magnetic valve III exports 3 ', two-position three way magnetic valve IV exports 4 ', two-position three way magnetic valve V exports 5 ', two-position three way magnetic valve VI export 6 ' with the outlet of gas analyzer J.
Draft tube a42 inlet end is communicated with nitrogen cylinder G, then sequential concatenation reduction valve H, spinner-type flowmeter I, draft tube b43 inlet end, hand valve b8, and is communicated with pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49 entrance.
Draft tube b43 porch is provided with hand valve a7, and order is communicated with gas outlet a23, gas outlet b24, gas outlet c25, gas outlet d26, gas outlet e27, gas outlet f28.
Gas outlet a23 entrance exports 1 ' with two-position three way magnetic valve I and is communicated with, gas outlet a23 outlet is communicated with fermentation tank I A entrance, be communicated with gas outlet g29 between gas outlet a23 entrance and gas outlet a23 export, be provided with hand valve i 15, be communicated with draft tube b43, gas outlet g29 is provided with hand valve c9, fermentation tank I A outlet is communicated with gas outlet n36 entrance, and gas outlet n36 outlet is communicated with two-position three way magnetic valve I entrance 1 through pipeline a44.
Gas outlet b24 entrance exports 2 ' with two-position three way magnetic valve II and is communicated with, gas outlet b24 outlet is communicated with fermentation tank II B entrance, be communicated with gas outlet h30 between gas outlet b24 entrance and gas outlet b24 export, be provided with hand valve j 16, be communicated with draft tube b43, gas outlet h30 is provided with hand valve d10, fermentation tank II B outlet is communicated with gas outlet o37 entrance, and gas outlet o37 outlet is communicated with two-position three way magnetic valve II entrance 2 through pipeline b45.
Gas outlet c25 entrance exports 3 ' with two-position three way magnetic valve III and is communicated with, gas outlet c25 outlet is communicated with fermentation tank III C entrance, be communicated with gas outlet i31 between gas outlet c25 entrance and gas outlet c25 export, be provided with hand valve k17, be communicated with draft tube b43, gas outlet i31 is provided with hand valve e11, fermentation tank III C outlet is communicated with gas outlet p38 entrance, and gas outlet p38 outlet is communicated with two-position three way magnetic valve III entrance 3 through pipeline c46.
Gas outlet d26 entrance exports 4 ' with two-position three way magnetic valve IV and is communicated with, gas outlet d26 outlet is communicated with fermentation tank IV D entrance, be communicated with gas outlet j32 between gas outlet d26 entrance and gas outlet d26 export, be provided with hand valve l 18, be communicated with draft tube b43, gas outlet j32 is provided with hand valve f12, fermentation tank IV D outlet is communicated with gas outlet q39 entrance, and gas outlet q39 outlet is communicated with two-position three way magnetic valve IV entrance 4 through pipeline d47.
Gas outlet e27 entrance exports 5 ' with two-position three way magnetic valve V and is communicated with, gas outlet e27 outlet is communicated with fermentation tank V E entrance, be communicated with gas outlet k33 between gas outlet e27 entrance and gas outlet e27 export, be provided with hand valve m19, be communicated with draft tube b43, gas outlet k33 is provided with hand valve g13, fermentation tank V E outlet is communicated with gas outlet r40 entrance, and gas outlet r40 outlet is communicated with two-position three way magnetic valve V entrance 5 through pipeline e48.
Gas outlet f28 entrance exports 6 ' with two-position three way magnetic valve VI and is communicated with, gas outlet f28 outlet is communicated with fermentation tank VI F entrance, be communicated with gas outlet l34 between gas outlet f28 entrance and gas outlet f28 export, be provided with hand valve n20, be communicated with draft tube b43, gas outlet l34 is provided with hand valve h14, fermentation tank VI F outlet is communicated with gas outlet s41 entrance, and gas outlet s41 outlet is communicated with two-position three way magnetic valve VI entrance 6 through pipeline f49.
Two-position three way magnetic valve I entrance 1, two-position three way magnetic valve II entrance 2, two-position three way magnetic valve III entrance 3, two-position three way magnetic valve IV entrance 4, two-position three way magnetic valve V entrance 5, two-position three way magnetic valve VI entrance 6 two-position three way magnetic valve I exports 1 ', two-position three way magnetic valve II exports 2 ', two-position three way magnetic valve III exports 3 ', two-position three way magnetic valve IV exports 4 ', two-position three way magnetic valve V exports 5 ', export 6 ' with two-position three way magnetic valve VI to control by microcomputer L.
Described gas outlet a23 exports export with the spacing at the bottom of fermentation tank I A tank, gas outlet b24 export with the spacing at the bottom of fermentation tank II B tank, gas outlet c25 and export with the spacing at the bottom of fermentation tank III C tank, gas outlet d26 and spacing at the bottom of fermentation tank IV D tank, gas outlet e27 export and spacing at the bottom of fermentation tank V E tank, gas outlet f28 export and spacing at the bottom of fermentation tank VI F tank is 1cm.
Described gas outlet n36 entrance and the spacing at the bottom of fermentation tank I A tank, gas outlet o37 entrance and the spacing at the bottom of fermentation tank II B tank, gas outlet p38 entrance and the spacing at the bottom of fermentation tank III C tank, gas outlet q39 entrance and the spacing at the bottom of fermentation tank IV D tank, gas outlet r40 entrance and the spacing at the bottom of fermentation tank V E tank, gas outlet s41 entrance and the spacing at the bottom of fermentation tank VI F tank are 20cm.
The material of described fermentation tank I A, fermentation tank II B, fermentation tank III C, fermentation tank IV D, fermentation tank V E, fermentation tank VI F is Bai Gang.
Good effect of the present utility model is:
During for high-speed simulation simulation on-line checkingi ruminant tumor gastric juice anaerobic fermentation, the CO of generation
2and CH
4, through years of researches, develop platform " 6 passage rumen fluid In Vitro Fermentation aerogenesis detection system " of informing against.To the system have been gas (CH
4, CO
2) the interior mark of the recovery and outer target dual test and the quantitative detection experiment to gas production rate during rumen fluid In Vitro Fermentation; Liquid in sheep rumen In Vitro Fermentation gas production rate and live body sheep gas production rate also compare just to be tested.Result shows: this system gas recovery is more than 98%; The test of rumen fluid In Vitro Fermentation shows, the CO produced when truly quantitatively can detect rumen fluid anaerobic fermentation online
2, CH
4amount.Reach a conclusion thus: this systems technology flow process is advanced, and testing result accurately and reliably.
Accompanying drawing explanation
Fig. 1 is 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting system structural representations
Fig. 2 is the pneumatic diagram of the marked gas recovery
Wherein: A. fermentation tank I B. fermentation tank II C. fermentation tank III D. fermentation tank IV E. fermentation tank V F. fermentation tank VI G. nitrogen cylinder H. reduction valve I. spinner-type flowmeter J. gas analyzer K. data acquisition control instrument L. microcomputer 1. two-position three way magnetic valve I entrance 2. two-position three way magnetic valve II entrance 3. two-position three way magnetic valve III entrance 4. two-position three way magnetic valve IV entrance 5. two-position three way magnetic valve V entrance 6. two-position three way magnetic valve VI entrance; 1 '. two-position three way magnetic valve I exports; 2 '. two-position three way magnetic valve II exports; 3 '. two-position three way magnetic valve III exports; 4 '. two-position three way magnetic valve IV exports; 5 '. two-position three way magnetic valve V exports; 6 '. two-position three way magnetic valve VI exports 7. hand valve a 8. hand valve b 9. hand valve c 10. hand valve d 11. hand valve e 12. hand valve f 13. hand valve g 14. hand valve h15. hand valve i 16. hand valve j 17. hand valve k 18. hand valve l 19. hand valve m 20. hand valve n 21. hand valve o 22. hand valve p 23. gas outlet a 24. gas outlet b 25. gas outlet c 26. gas outlet d 27. gas outlet e28. gas outlet f 29. gas outlet g 30. gas outlet h 31. gas outlet i 32. gas outlet j 33. gas outlet k 34. gas outlet l 35. gas outlet m 36. gas outlet n 37. gas outlet o 38. gas outlet p 39. gas outlet q 40. gas outlet r 41. gas outlet s 42. draft tube a 43. draft tube b 44. pipeline a 45. pipeline b 46. pipeline c 47. pipeline d 48. pipeline e 49. pipeline f 50. pipeline g
Embodiment
In mensuration In Vitro Fermentation gas production rate, before namely adding rumen fluid, be first filled with nitrogen, for anaerobic fermentation does early-stage preparations by each gas circuit (referring to all pipelines) of this system.
1. fermentation tank I A, fermentation tank II B, fermentation tank III C, fermentation tank IV D, fermentation tank V E, fermentation tank VI F are white steel fermentation tank (400ml volume), and with rib collar lid above each fermentation tank, lid mouth is connected with draft tube and gas outlet,
Each draft tube (entering the tracheae of fermentation tank) outlet is 1cm with the spacing at the bottom of each fermentation tank, and its effect is by fermentation liquor in the process passing into nitrogen (200ml/min), the methane that rumen fluid fermentation is produced and CO
2take out of.
Each venting conduit inlet and the spacing at the bottom of each fermentation tank are 20cm, and its effect is by the methane in fermentation liquor and CO
2gas, be transported in gas analyzer J by gas outlet n36, gas outlet o37, gas outlet p38, gas outlet q39, gas outlet r40, gas outlet s41, draft tube a42, draft tube b43, pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50.
During inflated with nitrogen: pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50 are as admission line.
During mensuration: pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50 are as gas exhaust duct.
2. be connected with 6mm high-voltage tube between nitrogen cylinder G with reduction valve H, other all connect with the silicone tube of 6mm.
3. detect in fermentation tank and produce gas flow (CO
2, CH
4) operation comprise the following steps:
(1-1) inflated with nitrogen order in pipeline: in the pipeline of fermentation tank I A, fermentation tank II B, fermentation tank III C, fermentation tank IV D, fermentation tank V E, fermentation tank VI F during inflated with nitrogen, open gas analyzer J (CO
2, CH
4) and the power switch of data acquisition control instrument K, open microcomputer L, click data gathers the control software design of controller K, starts experiment.
During inflated with nitrogen: pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50 are as admission line.
During mensuration: pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50 are as gas exhaust duct.
Start experiment:
When nitrogen cylinder G is to fermentation tank inflated with nitrogen: through draft tube a42, reduction valve H, spinner-type flowmeter I, draft tube b43, hand valve a7, gas outlet n36, gas outlet o37, gas outlet p38, gas outlet q39, gas outlet r40, gas outlet s41, pipeline a44, pipeline b45, pipeline c46, pipeline d47, pipeline e48, pipeline f49, pipeline g50, be transported in gas analyzer J.
Exhaust is passed through: gas outlet m35, gas outlet a23, gas outlet b24, gas outlet c25, gas outlet d26, gas outlet e27, gas outlet f28, gas outlet g29, gas outlet h30, gas outlet i31, gas outlet j32, gas outlet k33, gas outlet l34 pipeline discharge (emptying).
(1-2) open successively: the reduction valve H switch of nitrogen cylinder G, and regulate spinner-type flowmeter I flow (scope: between 200 ~ 300ml/min), hand valve b8, hand valve c9, hand valve d10, hand valve e11, hand valve f12, hand valve g13, hand valve h14;
(1-3) close successively: hand valve a7, hand valve i15, hand valve j16, hand valve k17, hand valve l18, hand valve m19, hand valve n20, hand valve o21, hand valve p22, by microcomputer L, by data acquisition control instrument K control software design program, by the time interval of 60 seconds circulation primary, gather the gas data of fermentation tank A, fermentation tank B, fermentation tank C, fermentation tank D, fermentation tank E, fermentation tank F respectively;
(1-4) when gathering fermentation tank A gas, two-position three way magnetic valve I is controlled automatically to open by microcomputer L,
When gathering fermentation tank B gas, two-position three way magnetic valve II is controlled automatically to open by microcomputer L,
When gathering fermentation tank C gas, two-position three way magnetic valve III is controlled automatically to open by microcomputer L,
When gathering fermentation tank D gas, two-position three way magnetic valve IV is controlled automatically to open by microcomputer L,
When gathering fermentation tank E gas, two-position three way magnetic valve V is controlled automatically to open by microcomputer L,
When gathering fermentation tank F gas, two-position three way magnetic valve VI is controlled automatically to open by microcomputer L, and six fermentation tank inflated with nitrogen 30min, operation completes.
(2) start to detect gas (CO
2, CH
4) record experiment:
(2-1) air intake opening of each fermentation tank and exhausr port and corresponding each draft tube and gas outlet is connected;
(2-2) close hand valve b8, open hand valve a7;
(2-3) by microcomputer L, utilize the control software design program of data acquisition control instrument K, by the time interval of 60 seconds circulation primary, gather the gas data of fermentation tank A, fermentation tank B, fermentation tank C, fermentation tank D, fermentation tank E, fermentation tank F respectively.
(2-4) when gathering fermentation tank A gas, two-position three way magnetic valve I is controlled automatically to open by microcomputer L,
When gathering fermentation tank B gas, two-position three way magnetic valve II is controlled automatically to open by microcomputer L,
When gathering fermentation tank C gas, two-position three way magnetic valve III is controlled automatically to open by microcomputer L,
When gathering fermentation tank D gas, two-position three way magnetic valve IV is controlled automatically to open by microcomputer L,
When gathering fermentation tank E gas, two-position three way magnetic valve V is controlled automatically to open by microcomputer L,
When gathering fermentation tank F gas, two-position three way magnetic valve VI is controlled automatically to open by microcomputer L.
(3) tested, opened hand valve o21 and hand valve p22, closed reduction valve H.
General layout Plan
The technical standard that this type systematic is ununified in the world at present, on the basis with reference to batch cultivation system and respiratory calorimetry apparatus, the utility model designs and have developed 6 passage rumen fluid In Vitro Fermentation aerogenesis detection systems, for fast and convenient on-line determination rumen fluid greenhouse gas emissions provide novel means easily.For this art establishment of standard in the future, provide reliable theoretical foundation.
The function of this system:
(1) adopt near infrared sensing technology, can realize the instantaneous generation of 24h online the real time measure gas, the pressure in fermentation tank is between 0.2-1.0KPa.Prevent the accuracy affecting measurement result after fermenting because of gas buildup, real Data support can be provided for research reduces greenhouse gases generation.
(2) with open circuit formula respiratory calorimetry apparatus principle basic simlarity, by O
2, CO
2and CH
4the list cover sensor of three kinds of gases, by the Integration ofTechnology of complexity, is merged into the gas analyzer of 6 passage one.
(3) every 6min circle collection once.The gas detect of each fermentation tank and collection, control by software workstation.Software workstation by 6 fermentation tanks coding (A-F) sequentially, gather data in a fermentation tank once at interval of 60s, and according to the automatic computing of the formula that program inediting is good and preserve gather detest O
2amount, CO
2output and CH
4discharge capacity data.Continual supply N during gas air-channel system plant running
2, ensure that whole sweat is all in anaerobic environment.
The inspection of gas reclaiming rate
Test in the process of carrying out at rumen fluid In Vitro Fermentation, adopt external standard method (to use calibrating gas CO respectively
2and CH
4demarcate) and internal standard method (mixed gas of the outer fermentation gas of calibrating gas and rumen fluid) do gas reclaiming rate dual test.
1. external standard method: close M, N valve, open O valve, the pipeline gas flow that wherein O valve controls is 200mL/min, bioassay standard gas concentration.
The recovery (%)=a/b × 100%
A: actual on-line determination gas concentration value;
B: the concentration value of calibrating gas.
2. internal standard method: first open M, N valve, close O valve, the pipeline gas flow that wherein M, N valve controls is 200mL/min, measures the gas concentration value in fermentation tank; Then C valve is opened, the pipeline gas flow that M, N valve and O valve control is adjusted to 100mL/min, the gas concentration value in Simultaneously test standard gas concentration value and fermentation tank.
The recovery (%)=(b-a)/c × 100%
A: open M, N valve, actual on-line determination concentration value during closedown O valve;
Actual on-line determination concentration value when b:M, N valve and O valve are opened simultaneously;
C: the concentration value of calibrating gas.
Rumen fluid In Vitro Fermentation is tested
Single factor design is taked in this test, carries out In Vitro Fermentation by 6 passage rumen fluid In Vitro Fermentation aerogenesis detection systems of the present utility model, is the white steel fermentation tank 6 of 200ml and the constant temperature oscillator of energy precise control of temperature with volume.6 passage rumen fluid In Vitro Fermentation aerogenesis detection systems, before on-test, continue to pass into N in 30 minutes
2, carry out in the environment of anaerobism with warranty test: white steel fermentation tank was placed in constant temperature oscillator in first 30 minutes of on-test, to ensure that its temperature maintains 39 DEG C.In every morning 6:30 feed daily ration after two hours, rumen fluid device will be got with pump bowl, respectively from every bellwether (after feeding and butchering, learn that the volume of 50kg sheep cud is about 8L) extract the rumen fluid 450ml altogether of 150ml in cud, mix and be placed in the bottle,suction of isothermal holding.
Take 1g sheep's hay and 1g fine fodder respectively, put into fermenter base.Inject 50ml rumen fluid in each fermentation tank, closed fermentation tank ferments.Ferment to the CH of data analyzer collection
4till value zero.
The determination test of live body sheep aerogenesis
This test adopts single factor design, with respiratory calorimetry apparatus by thick than being 4:6 for each issue experiment sheep essence, raising 7 days in advance, after weighing by head, putting into breath heat measurement indoor, carrying out breath heat measurement experiment in 24 hours, weighs to lead by head and tests into breath heat measurement room in interval for 3 days again.Microcomputer records consumption O during every bellwether respiratory metabolism automatically
2amount, CO
2discharge capacity, CH
4discharge capacity, respiratory quotient, temperature, humidity, enter to arrange gas flow data etc. in respiratory chamber.
Rumen fluid In Vitro Fermentation gas production rate and live body sheep respiratory calorimetry apparatus measure the comparison sheet of gas production rate
Note: in the live body sheep body of In Vitro Fermentation estimation, gas yield (y)=fermentation tank injects Rumen volume (50ml) × In Vitro Fermentation gas production rate (x) ÷ live body sheep cud volume (8L)
The CH measured between rumen fluid In Vitro Fermentation aerogenesis pick-up unit and live body sheep respiratory calorimetry apparatus as seen from table
4discharge capacity is the relation of about 1.5 times, CO
2gas production rate is the relation of about 1 times.The CH that In Vitro Fermentation device detects
4value determines CH higher than live body sheep respiratory calorimetry apparatus
4discharge capacity, its reason may be the batch cultivation system that this In Vitro Fermentation device belongs to a small amount of rumen fluid short-term dynamic open type, has not both had semi-permeable diaphragm and rumen bypass effect, and has not also had the function of fatty acid absorption.And live body sheep Rumen Internal Environment belongs to continuity fermentation and absorb the culture systems of fatty acid, what not only there is substrate and damping fluid enters the continuous discharge with chyme continuously, also there is microorganism steady-state system in cud, microorganism is constantly multiplied, and maintains the environment that microorganism depends on for existence.The CO that In Vitro Fermentation device detects
2value is less than the CO that live body sheep respiratory calorimetry apparatus determines
2generation is then the CO measured because of In Vitro Fermentation device
2value is produced by rumen fluid fermentation completely, and the CO recorded in live body sheep respiratory calorimetry apparatus
2value is the value that sheep self pulmonary respiration and physiological activity produce, the CO that lumen fermentation produces
2a just part wherein, and draw increase CO with sheep body weight according to live body sheep respiratory calorimetry apparatus
2output increases.Therefore, although rumen fluid In Vitro Fermentation device can measure the instantaneous gas production rate of rumen fluid, under the impact of factors cannot completely and the gas production rate that goes out with breath heat measurement system measurement of live body sheep coincide.On the whole, data present relevant.Khazaal etc. (1993) use In Vitro Fermentation method and Nylon Bag comparative experiments to show that In Vitro Fermentation device and intracorporal method are substantially close, Zhang Wenlu etc. have high correlation both being proposed by the correlativity of roughage dry matter degradability research In Vitro Fermentation and Nylon Bag, and In Vitro Fermentation method as fast, can measure rumen digestion qualitatively.In the external body of this experiment, contrast test is consistent with the research of Khazaal, Zhang Wenlu etc., and both gained gas values are basically identical, can as the installation for fermenting of quantitative and qualitative analysis mensurated gas composition discharge.
Claims (4)
1. 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems, is characterized in that by fermentation tank I (A), fermentation tank II (B), fermentation tank III (C), fermentation tank IV (D), fermentation tank V (E), fermentation tank VI (F), nitrogen cylinder (G), reduction valve (H), spinner-type flowmeter (I), gas analyzer (J), data acquisition control instrument (K), microcomputer (L), two-position three way magnetic valve I entrance (1), two-position three way magnetic valve II entrance (2), two-position three way magnetic valve III entrance (3), two-position three way magnetic valve IV entrance (4), two-position three way magnetic valve V entrance (5), two-position three way magnetic valve VI entrance (6), two-position three way magnetic valve I exports (1 '), two-position three way magnetic valve II exports (2 '), two-position three way magnetic valve III exports (3 '), two-position three way magnetic valve IV exports (4 '), two-position three way magnetic valve V exports (5 '), two-position three way magnetic valve VI exports (6 '), hand valve a (7), hand valve b (8), hand valve c (9), hand valve d (10), hand valve e (11), hand valve f (12), hand valve g (13), hand valve h (14), hand valve i (15), hand valve j (16), hand valve k (17), hand valve l (18), hand valve m (19), hand valve n (20), hand valve o (21), hand valve p (22), gas outlet a (23), gas outlet b (24), gas outlet c (25), gas outlet d (26), gas outlet e (27), gas outlet f (28), gas outlet g (29), gas outlet h (30), gas outlet i (31), gas outlet j (32), gas outlet k (33), gas outlet l (34), gas outlet m (35), gas outlet n (36), gas outlet o (37), gas outlet p (38), gas outlet q (39), gas outlet r (40), gas outlet s (41), draft tube a (42), draft tube b (43), pipeline a (44), pipeline b (45), pipeline c (46), pipeline d (47), pipeline e (48), pipeline f (49) and pipeline g (50) composition, wherein gas analyzer (J) is connected with microcomputer (L) through data acquisition control instrument (K), pipeline g (50) porch is provided with hand valve o (21), and is communicated with the entrance of gas analyzer (J) through two-position three way magnetic valve I entrance (1), two-position three way magnetic valve II entrance (2), two-position three way magnetic valve III entrance (3), two-position three way magnetic valve IV entrance (4), two-position three way magnetic valve V entrance (5), two-position three way magnetic valve VI entrance (6), gas outlet m (35) exit is provided with hand valve p (22), and export (1 ') through two-position three way magnetic valve I, two-position three way magnetic valve II exports (2 '), two-position three way magnetic valve III exports (3 '), two-position three way magnetic valve IV exports (4 '), two-position three way magnetic valve V exports (5 '), two-position three way magnetic valve VI exports (6 ') and gas analyzer (J) outlet, draft tube a (42) inlet end is communicated with nitrogen cylinder (G), then sequential concatenation reduction valve (H), spinner-type flowmeter (I), draft tube b (43) inlet end, hand valve b (8), and be communicated with pipeline a (44), pipeline b (45), pipeline c (46), pipeline d (47), pipeline e (48), pipeline f (49) entrance, draft tube b (43) porch is provided with hand valve a (7), and order is communicated with gas outlet a (23), gas outlet b (24), gas outlet c (25), gas outlet d (26), gas outlet e (27), gas outlet f (28), gas outlet a (23) entrance and two-position three way magnetic valve I export (1 ') and are communicated with, gas outlet a (23) outlet is communicated with fermentation tank I (A) entrance, gas outlet g (29) is communicated with between gas outlet a (23) entrance and gas outlet a (23) outlet, be provided with hand valve i (15), be communicated with draft tube b (43), gas outlet g (29) is provided with hand valve c (9), fermentation tank I (A) outlet is communicated with gas outlet n (36) entrance, gas outlet n (36) outlet is communicated with two-position three way magnetic valve I entrance (1) through pipeline a (44), gas outlet b (24) entrance and two-position three way magnetic valve II export (2 ') and are communicated with, gas outlet b (24) outlet is communicated with fermentation tank II (B) entrance, gas outlet h (30) is communicated with between gas outlet b (24) entrance and gas outlet b (24) outlet, be provided with hand valve j (16), be communicated with draft tube b (43), gas outlet h (30) is provided with hand valve d (10), fermentation tank II (B) outlet is communicated with gas outlet o (37) entrance, gas outlet o (37) outlet is communicated with two-position three way magnetic valve II entrance (2) through pipeline b (45), gas outlet c (25) entrance and two-position three way magnetic valve III export (3 ') and are communicated with, gas outlet c (25) outlet is communicated with fermentation tank III (C) entrance, gas outlet i (31) is communicated with between gas outlet c (25) entrance and gas outlet c (25) outlet, be provided with hand valve k (17), be communicated with draft tube b (43), gas outlet i (31) is provided with hand valve e (11), fermentation tank III (C) outlet is communicated with gas outlet p (38) entrance, gas outlet p (38) outlet is communicated with two-position three way magnetic valve III entrance (3) through pipeline c (46), gas outlet d (26) entrance and two-position three way magnetic valve IV export (4 ') and are communicated with, gas outlet d (26) outlet is communicated with fermentation tank IV (D) entrance, gas outlet j (32) is communicated with between gas outlet d (26) entrance and gas outlet d (26) outlet, be provided with hand valve l (18), be communicated with draft tube b (43), gas outlet j (32) is provided with hand valve f (12), fermentation tank IV (D) outlet is communicated with gas outlet q (39) entrance, gas outlet q (39) outlet is communicated with two-position three way magnetic valve IV entrance (4) through pipeline d (47), gas outlet e (27) entrance and two-position three way magnetic valve V export (5 ') and are communicated with, gas outlet e (27) outlet is communicated with fermentation tank V (E) entrance, gas outlet k (33) is communicated with between gas outlet e (27) entrance and gas outlet e (27) outlet, be provided with hand valve m (19), be communicated with draft tube b (43), gas outlet k (33) is provided with hand valve g (13), fermentation tank V (E) outlet is communicated with gas outlet r (40) entrance, gas outlet r (40) outlet is communicated with two-position three way magnetic valve V entrance (5) through pipeline e (48), gas outlet f (28) entrance and two-position three way magnetic valve VI export (6 ') and are communicated with, gas outlet f (28) outlet is communicated with fermentation tank VI (F) entrance, gas outlet l (34) is communicated with between gas outlet f (28) entrance and gas outlet f (28) outlet, be provided with hand valve n (20), be communicated with draft tube b (43), gas outlet l (34) is provided with hand valve h (14), fermentation tank VI (F) outlet is communicated with gas outlet s (41) entrance, gas outlet s (41) outlet is communicated with two-position three way magnetic valve VI entrance (6) through pipeline f (49), two-position three way magnetic valve I entrance (1), two-position three way magnetic valve II entrance (2), two-position three way magnetic valve III entrance (3), two-position three way magnetic valve IV entrance (4), two-position three way magnetic valve V entrance (5), two-position three way magnetic valve VI entrance (6) two-position three way magnetic valve I exports (1 '), two-position three way magnetic valve II exports (2 '), two-position three way magnetic valve III exports (3 '), two-position three way magnetic valve IV exports (4 '), two-position three way magnetic valve V exports (5 ') and two-position three way magnetic valve VI and exports (6 ') and control by microcomputer (L).
2. by 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems according to claim 1, it is characterized in that described gas outlet a (23) outlet and the spacing at the bottom of fermentation tank I (A) tank, gas outlet b (24) outlet and the spacing at the bottom of fermentation tank II (B) tank, gas outlet c (25) outlet and the spacing at the bottom of fermentation tank III (C) tank, gas outlet d (26) outlet and the spacing at the bottom of fermentation tank IV (D) tank, gas outlet e (27) outlet and the spacing at the bottom of fermentation tank V (E) tank, gas outlet f (28) outlet is 1cm with the spacing at the bottom of fermentation tank VI (F) tank.
3. by 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems according to claim 1, it is characterized in that described gas outlet n (36) entrance and the spacing at the bottom of fermentation tank I (A) tank, gas outlet o (37) entrance and the spacing at the bottom of fermentation tank II (B) tank, gas outlet p (38) entrance and the spacing at the bottom of fermentation tank III (C) tank, gas outlet q (39) entrance and the spacing at the bottom of fermentation tank IV (D) tank, gas outlet r (40) entrance and the spacing at the bottom of fermentation tank V (E) tank, gas outlet s (41) entrance and the spacing at the bottom of fermentation tank VI (F) tank are 20cm.
4., by 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems according to claim 1, it is characterized in that the material of described fermentation tank I (A), fermentation tank II (B), fermentation tank III (C), fermentation tank IV (D), fermentation tank V (E), fermentation tank VI (F) is Bai Gang.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498357A (en) * | 2015-01-14 | 2015-04-08 | 吉林省农业科学院 | 6-channel rumen fluid external fermentation aerogenesis online detection system |
| CN105301198A (en) * | 2015-11-11 | 2016-02-03 | 中国科学院武汉岩土力学研究所 | Anaerobic digestion gas automatic detection system |
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2015
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498357A (en) * | 2015-01-14 | 2015-04-08 | 吉林省农业科学院 | 6-channel rumen fluid external fermentation aerogenesis online detection system |
| CN104498357B (en) * | 2015-01-14 | 2016-05-18 | 吉林省农业科学院 | 6 passage rumen fluid In Vitro Fermentation aerogenesis on-line detecting systems |
| CN105301198A (en) * | 2015-11-11 | 2016-02-03 | 中国科学院武汉岩土力学研究所 | Anaerobic digestion gas automatic detection system |
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