CN208022990U - Rumen fermentation in vitro device - Google Patents
Rumen fermentation in vitro device Download PDFInfo
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- CN208022990U CN208022990U CN201721660366.7U CN201721660366U CN208022990U CN 208022990 U CN208022990 U CN 208022990U CN 201721660366 U CN201721660366 U CN 201721660366U CN 208022990 U CN208022990 U CN 208022990U
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- round
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- air bleeding
- pressure reducing
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Abstract
A kind of rumen fermentation in vitro device is related to ruminant feed evaluation of nutrition technical field, and round, which is placed in thermostatic control oscillator vibration, simulates rumen peristalsis；Round oral area is equipped with sealing structure, and sealing structure is equipped with intake valve, air bleeding valve and pressure reducing valve, and pressure reducing valve is for making the air pressure in round will not be excessively high；Aforementioned air bleeding valve is collected with aerogenesis by gas piping and recording device is connected, and aforementioned intake valve is connected by gas piping with anaerobism air source；Anaerobism air source is used to exclude the air in round, is at anaerobic environment.The utility model opens or closes air bleeding valve by selection, to quickly be switched to two different experimental provisions, improves the utilization rate of equipment；Simultaneously equipped with method is individually depressurized, avoiding traditional sealing-plug when carrying out simple digestion method experiment is popped up the negative effect brought by high pressure gas.
Description
Technical field：
The utility model is related to ruminant feed evaluation of nutrition technical field, more particularly to a kind of rumen fermentation in vitro
Device.
Background technology：
Ruminant domestic animal feed includes leguminous forage, graminous pasture and various stalk coarse fodders and various concentrated feeds, evaluation
The index of its feeding value has conventional nutrients, digestibility, utilization rate and palatability of feed etc..Feed digestibility is feed
The key index of assessment of nutritional value.Feed digestibility is influenced by factors, more important in addition to the factor in terms of animal, just
It is the factor in terms of feed.And different types of feed or feed different sources of the same race, different harvest times, at different processing
The factors such as reason can make digestibility, and there are larger differences.
Cud is the distinctive digestive organs of ruminant.The digestion method of nutriment is mainly that microorganism disappears in cud
Change.Rumen microorganism includes mainly protozoon (such as infusorian, flagellate), bacterium and a small amount of fungi, fiber especially therein
Decomposer is the critical bacterial populations in cud, is had a major impact to lumen fermentation.Specifically, rumen microorganism is in ruminant
In entire digestion process, have the advantages that two is big：First, the cellulose and hemicellulose and non-protein that cannot digest using host
The substances such as nitrogen (NPN) dramatically increase the amount of the availability and microprotein (MCP) of total energy (GE) in feed, improve animal
To the digestibility and utilization rate of feed；Second is that microorganism energy synth essential amino acid, it is necessary to the nutrition such as aliphatic acid and B family vitamin
Substance is utilized for host.Therefore, the advantage for playing rumen microorganism digestion effectively utilizes the high feed money of some fibre content
Source is one of the main contents of current regulating rumen.
The best method of research ruminant tumor gastric fermentation is tested using in vivo lumen fermentation.But due to live body
Interior experiment has period length, the problems such as animal is more, environmental condition is not easy to control, of high cost, people is needed constantly to explore more simple
Single, economic, reliable method.Therefore, various in vitro artificial rumen methods are come into being.Using it is relatively wide, be affected have production
Gas method (Menke etc., 1979), two-phase method (Tilley and Terry, 1963) and simple digestion method (Goering and Van
Soest, 1970) etc..These methods belong to batch culture (batch culture), i.e. microbial inoculant object and fermentation bottom
Object is added at one time, and after certain time cultivates, terminates culture within the set time.Wherein aerogenesis method and simple digestion method is mesh
It is preceding to utilize more rumen fermentation in vitro method.
Currently, a set of equipment is respectively adopted in aerogenesis method and simple digestion method, utilization rate of equipment and installations is low；In addition, simple digestion method
It ferments in airtight bottle, with the increase of gas production, the pressure in the bottle constantly increases, until bottle stopper top is flown, then by manually covering back,
Someone's post is on the one hand needed in this way, on the other hand destroys anaerobic environment, and the authenticity of experimental data is made to reduce.
Invention content：
The utility model is by common rumen fermentation in vitro aerogenesis method and simple digestion method knot in current process of scientific research
Altogether, it optimizes, a set of lumen fermentation device that can be carried out aerogenesis experiment but also simply be digested of design, together
When solve it is existing it is simple digestion subtraction unit defect.
A kind of rumen fermentation in vitro device, including：Round, water bath chader, thermostat, aerogenesis are collected and note
Recording device, intake valve, air bleeding valve, pressure reducing valve, anaerobism air source；Wherein, round is placed on water bath chader, water-bath oscillation
Device is simulated for rumen peristalsis, and thermostat is used to keep constant temperature for water-bath；Round oral area is equipped with sealing structure, sealing knot
Structure is equipped with intake valve, air bleeding valve and pressure reducing valve, so that extraneous gas is entered in round by intake valve, and make hair
Ferment gas in container can be discharged by air bleeding valve, and pressure reducing valve is for making the air pressure in round will not be excessively high；Aforementioned exhaust
Valve is collected with aerogenesis by gas piping and recording device is connected, and aforementioned intake valve is connected by gas piping with anaerobism air source；
Anaerobism air source is used to exclude the air in round, is at anaerobic environment.
Before lumen fermentation cultivation and fermentation, water bath chader is heated to 39 DEG C, culture substrate is added to round
In, round is sealed with sealing structure, is placed in water bath chader, air bleeding valve is opened, is then passed through by intake valve
Cud buffer solution is added to round after 1-2 minutes, round is sealed with sealing structure and is continually fed by anaerobic gas
Carbon dioxide 1-2 minutes.Later, it is closed at this time without gas collection and the recording device of practicing midwifery if carrying out simple digestion method experiment
Air bleeding valve is closed, can be depressurized automatically by pressure reducing valve when air pressure is excessive in round；It will exhaust if carrying out aerogenesis method experiment
Valve is collected with aerogenesis and recording device is connect, and records gas production at any time.
Preferably, sealing structure is sealing-plug, and intake valve, air bleeding valve and pressure reducing valve run through sealing-plug.
Preferably, sealing structure is the lid coordinated with round mouth thread, and intake valve, air bleeding valve and pressure reducing valve pass through
Wear lid.
Preferably, water bath chader is the thermostatic control oscillator vibration for being integrated with thermostat.
Preferably, anaerobism air source is CO2。
Preferably, round is conical flask.
Preferably, aerogenesis is collected and recording device is syringe.
Preferably, gas piping is emulsion tube.
The utility model opens or closes air bleeding valve by selection, to quickly be switched to two different experiment dresses
It sets, improves the utilization rate of equipment；Simultaneously equipped with individually decompression method, avoided when carrying out simple digestion method experiment traditional
Sealing-plug is popped up the negative effect brought by high pressure gas.
Description of the drawings：
Attached drawing 1 is the structural schematic diagram of the rumen fermentation in vitro device of a width better embodiment.
In figure：Round 1, water bath chader 2, aerogenesis is collected and recording device 3, intake valve 4, air bleeding valve 5, pressure reducing valve
6, anaerobism air source 7, sealing structure 11.
Specific implementation mode：
As shown in Fig. 1, a kind of rumen fermentation in vitro device, including：Round 1, water bath chader 2, constant temperature dress
It sets, aerogenesis is collected and recording device 3, intake valve 4, air bleeding valve 5, pressure reducing valve 6, anaerobism air source 7；Wherein, round 1 is placed on
On water bath chader 2, water bath chader 2 is simulated for rumen peristalsis, and thermostat is used to keep constant temperature for water-bath；Round
1 oral area is equipped with sealing structure 11, and sealing structure 11 is equipped with intake valve 4, air bleeding valve 5 and pressure reducing valve 6, extraneous gas is allow to lead to
It crosses intake valve 4 to enter in round 1, and gas in round 1 is allow to be discharged by air bleeding valve 5, pressure reducing valve 6 is used for
Make the air pressure in round 1 will not be excessively high；Aforementioned air bleeding valve 5 is collected with aerogenesis by gas piping and recording device 3 is connected,
Aforementioned intake valve 4 is connected by gas piping with anaerobism air source 7；Anaerobism air source 7 is used to exclude the air in round 1, makes
It is in anaerobic environment.
In the present embodiment, sealing structure 11 is sealing-plug, and intake valve 4, air bleeding valve 5 and pressure reducing valve 6 run through sealing-plug,
Water bath chader 2 is the thermostatic control oscillator vibration for being integrated with thermostat, and anaerobism air source 7 is CO2, round 1 is triangle burning
Bottle, aerogenesis is collected and recording device 3 is syringe, and gas piping is emulsion tube.
In another kind applies mode, sealing structure 11 is the lid coordinated with 1 mouth thread of round, intake valve 4, row
Air valve 5 and pressure reducing valve 6 run through lid.
Claims (8)
1. a kind of rumen fermentation in vitro device, which is characterized in that including：Round, water bath chader, thermostat, production
Gas is collected and recording device, intake valve, air bleeding valve, pressure reducing valve, anaerobism air source；Wherein, round is placed on water bath chader
On, water bath chader is simulated for rumen peristalsis, and thermostat is used to keep constant temperature for water-bath；Round oral area is equipped with sealing
Structure, sealing structure are equipped with intake valve, air bleeding valve and pressure reducing valve, extraneous gas are allow to enter round by intake valve
It is interior, and gas in round is allow to be discharged by air bleeding valve, pressure reducing valve is for making the air pressure in round will not mistake
It is high；Aforementioned air bleeding valve is collected by gas piping and aerogenesis and recording device is connected, aforementioned intake valve by gas piping with detest
Source of oxygen is connected；Anaerobism air source is used to exclude the air in round, is at anaerobic environment.
2. rumen fermentation in vitro device as described in claim 1, which is characterized in that sealing structure is sealing-plug, intake valve,
Air bleeding valve and pressure reducing valve run through sealing-plug.
3. rumen fermentation in vitro device as described in claim 1, which is characterized in that sealing structure be and round oral area
The lid of screw-thread fit, intake valve, air bleeding valve and pressure reducing valve run through lid.
4. the rumen fermentation in vitro device as described in claims 1 to 3 any one, which is characterized in that water bath chader is
It is integrated with the thermostatic control oscillator vibration of thermostat.
5. the rumen fermentation in vitro device as described in claims 1 to 3 any one, which is characterized in that anaerobism air source is CO2。
6. the rumen fermentation in vitro device as described in claims 1 to 3 any one, which is characterized in that round three
Angle flask.
7. the rumen fermentation in vitro device as described in claims 1 to 3 any one, which is characterized in that aerogenesis is collected and note
Recording device is syringe.
8. the rumen fermentation in vitro device as described in claims 1 to 3 any one, which is characterized in that gas piping is breast
Sebific duct.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721660366.7U CN208022990U (en) | 2017-12-04 | 2017-12-04 | Rumen fermentation in vitro device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721660366.7U CN208022990U (en) | 2017-12-04 | 2017-12-04 | Rumen fermentation in vitro device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208022990U true CN208022990U (en) | 2018-10-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721660366.7U Expired - Fee Related CN208022990U (en) | 2017-12-04 | 2017-12-04 | Rumen fermentation in vitro device |
Country Status (1)
| Country | Link |
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| CN (1) | CN208022990U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521862A (en) * | 2019-08-23 | 2019-12-03 | 安徽农业大学 | A kind of pannage and preparation method thereof carrying out ensiling using bamboo shoots leftover bits and pieces |
| CN114106989A (en) * | 2021-11-18 | 2022-03-01 | 黑龙江八一农垦大学 | Rumen in-vitro fermentation device and method thereof |
| CN114350475A (en) * | 2022-01-07 | 2022-04-15 | 河北农业大学 | Vacuum bag type rumen in-vitro fermentation device and operation method thereof |
-
2017
- 2017-12-04 CN CN201721660366.7U patent/CN208022990U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110521862A (en) * | 2019-08-23 | 2019-12-03 | 安徽农业大学 | A kind of pannage and preparation method thereof carrying out ensiling using bamboo shoots leftover bits and pieces |
| CN114106989A (en) * | 2021-11-18 | 2022-03-01 | 黑龙江八一农垦大学 | Rumen in-vitro fermentation device and method thereof |
| CN114350475A (en) * | 2022-01-07 | 2022-04-15 | 河北农业大学 | Vacuum bag type rumen in-vitro fermentation device and operation method thereof |
| CN114350475B (en) * | 2022-01-07 | 2023-07-18 | 河北农业大学 | Vacuum bag type rumen in-vitro fermentation device and operation method thereof |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181030 Termination date: 20191204 |