CN213624180U - Simulation fermentation cylinder biological reaction device - Google Patents
Simulation fermentation cylinder biological reaction device Download PDFInfo
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- CN213624180U CN213624180U CN202022441824.6U CN202022441824U CN213624180U CN 213624180 U CN213624180 U CN 213624180U CN 202022441824 U CN202022441824 U CN 202022441824U CN 213624180 U CN213624180 U CN 213624180U
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Abstract
The utility model discloses a simulation fermentation cylinder biological reaction unit belongs to biological fermentation treatment facility field. This device is including heat collection pot, regulation and control device and iron stand platform, the heat collection pot is including setting up agitator, stirring rake, the three-necked flask that heater, cooler and top-down in it connected gradually, regulation and control device includes bottom support, attemperator, and bottom support surface is provided with switch, adjust temperature knob and speed governing knob, and the right-hand air pump, the air flowmeter that are provided with of bottom support, the iron stand platform sets up in heat collection pot left and through I fixed connection agitator of universal clamp, through II fixed connection three-necked flask of universal clamp. The device utilizes the thermal-arrest pot to simulate the fermenter biological reaction, is provided with the regulation and control device, is convenient for experimental reaction parameter adjustment, is simple and convenient to operate, reduces the test cost, is suitable for small-scale fermentation of different resting cells, and is convenient for popularization and use in the microbial fermentation experimental research stage.
Description
Technical Field
The utility model belongs to biological fermentation treatment facility field, concretely relates to simulation fermentation cylinder biological reaction unit.
Background
Microbial fermentation is a process of converting a raw material into a product desired by a human through a specific metabolic pathway using some specific functions of a microorganism under appropriate conditions. The production level of microbial fermentation mainly depends on the genetic characteristics and culture conditions of strains, and also depends on that various parameters in the fermentation process are in a constantly changing state, and the key point is process detection and control. The fermentation engineering is widely applied to: the medicine industry, the food industry, the energy industry, the chemical industry, the agriculture, the environmental protection and the like.
The method utilizes resting cells to carry out steroid conversion, belongs to the field of microbial fermentation, utilizes the resting cells to carry out biological fermentation, has the advantages of simple microbial culture process, high conversion efficiency, high product yield and simple components of conversion liquid, is beneficial to product separation and purification, can artificially adjust the concentration of thalli in the conversion process to shorten the production time, saves a large amount of energy and production cost, and is beneficial to popularization.
However, in the existing microbial fermentation process, the fermentation tank needs more equipment, has a complex structure, a large volume, complex operation and high cost, and is not suitable for popularization and use in the test stage.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides an analog fermentation jar bioreactor, the device utilize thermal-arrest pot analog fermentation jar biological reaction, simple structure, area is little to be provided with the experimental reaction parameter regulation of being convenient for of regulation and control device, easy and simple to handle, it is convenient to wash, has reduced test cost, is applicable to different resting cell small-scale fermentations, the use widely of the experimental research stage of microbial fermentation of being convenient for.
The utility model provides a simulated fermentation tank biological reaction device, which comprises,
the heat collection pot is used for simulating the biological reaction of the fermentation tank;
the regulating device is used for regulating and controlling a power switch, the reaction temperature, the stirring speed and the oxygen introduction amount;
the iron stand is used for installing the heat collection pot and fixing the heat collection pot above the regulating device;
wherein:
the heat collection pot comprises a heater, a cooler, a stirrer, a stirring paddle and a three-neck flask which are arranged in the heat collection pot, and the stirrer, the stirring paddle and the three-neck flask are sequentially connected from top to bottom;
the regulating device comprises a bottom support and a temperature regulating device, a power switch, a temperature regulating knob and a speed regulating knob are arranged on the outer surface of the bottom support, and an air pump and an air flow meter are arranged on the right side of the bottom support;
the iron stand is arranged at the left side of the heat collecting pot and is fixedly connected with the stirrer through a universal clamp I and fixedly connected with the three-neck flask through a universal clamp II.
Further, the air pump is connected with the three-neck flask through a conduit, and the air flow meter is arranged on the conduit.
Furthermore, a heat dissipation plate is arranged below the heat collection pot, and a bottom support is connected below the heat dissipation plate.
Furthermore, the universal clamp I and the universal clamp II are both provided with adjusting thread knobs, the stirrer is fixedly connected with a stirring paddle through the adjusting thread knobs of the universal clamp I, and the stirring paddle is fixedly connected with the three-neck flask through the adjusting thread knobs of the universal clamp II.
Further, the stirring paddle is connected with the three-neck flask through a bottle stopper with a hole.
Further, the temperature adjusting device is a temperature control heater.
Compared with the prior art, the technical scheme of the utility model, beneficial effect is as follows:
the utility model relates to an analog fermentation jar biological reaction device, the device utilize thermal-arrest pot analog fermentation jar to carry out biological reaction, and the thermal-arrest pot is adjusted attemperator, agitator and air pump respectively through adjust temperature knob, speed governing knob and air flowmeter to this regulates and control reaction temperature, stirring speed and logical oxygen volume respectively, reaches the purpose that in time carries out process detection and regulation control.
The utility model discloses utilize thermal-arrest pot analog fermentation jar biological reaction, simple structure, area is little to be provided with the experimental reaction parameter regulation of being convenient for of regulation and control device, have advantages such as mix abundant, the business turn over material is convenient, fermentation effect is good, easy and simple to handle, it is convenient to wash, has reduced the test cost, is applicable to different resting cell small-scale fermentations, the using widely of the experimental research stage of microbial fermentation of being convenient for.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a simulated fermenter bioreactor of the present invention;
in the figure: 1-heat collecting pot, 2-iron stand, 3-heater, 4-cooler, 5-stirrer, 6-stirring paddle, 7-three-neck flask, 8-perforated bottle stopper, 9-heat dissipation plate, 10-bottom support, 11-temperature adjusting device, 12-power switch, 13-temperature adjusting knob, 14-speed adjusting knob, 15-air pump, 16-air flow meter, 17-conduit, 18-universal clamp I, 19-universal clamp II and 20-adjusting screw thread knob.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; may be a mechanical connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Referring to FIG. 1, the present embodiment provides a simulated fermenter bioreactor, comprising,
the heat collection pot 1 is used for simulating the biological reaction of a fermentation tank;
the regulating device is used for regulating and controlling a power switch, the reaction temperature, the stirring speed and the oxygen introduction amount;
and the iron support 2 is used for installing the heat collecting pot 1 and fixing the heat collecting pot 1 above the regulating device.
The heat collection pot 1 comprises a heater 3, a cooler 4 and a stirrer 5, a stirring paddle 6 and a three-neck flask 7 which are sequentially connected from top to bottom, wherein the heater 3, the cooler 4 and the stirrer 5 are arranged in the heat collection pot, the stirring paddle 6 is connected with the three-neck flask 7 through a bottle stopper 8 with holes, a heat dissipation plate 9 is arranged below the heat collection pot 1, the device can be cooled through the heat dissipation plate 9, the instrument is prevented from being burnt out due to long-time use, and a bottom support 10 is connected below the heat dissipation plate 9.
The regulating and controlling device comprises a bottom support 10 and a temperature regulating device 11, a power switch 12, a temperature regulating knob 13 and a speed regulating knob 14 are arranged on the outer surface of the bottom support 10, an air pump 15 and an air flow meter 16 are arranged on the right side of the bottom support 10, the air pump 15 is connected with the three-neck flask 7 through a guide pipe 17, and the air flow meter 16 is arranged on the guide pipe 17.
The iron stand platform 2 is arranged on the left of the heat collecting pot 1 and is fixedly connected with a stirrer through a universal clamp I18, the three-neck flask 7 is fixedly connected with a universal clamp II 19, the universal clamp I18 and the universal clamp II 19 are respectively provided with an adjusting thread knob 20, the stirrer 5 is fixedly connected with a stirring paddle 6 through the adjusting thread knob 20 of the universal clamp I18, the stirring paddle 6 is fixedly connected with the three-neck flask 7 through the adjusting thread knob 20 of the universal clamp II 19, and the adjusting thread knob 20 is used for preventing the stirrer 5 and the three-neck flask 7 from falling off during biotransformation reaction and influencing the biotransformation reaction.
In one embodiment of the present application, the temperature regulating device is a temperature controlled heater.
Before conversion reaction, the stirrer 5 and the three-neck flask 7 are adjusted to be in the same vertical direction, the three-neck flask 7 is fixed on the iron support 2 by adjusting an adjusting screw knob 20 of the universal clamp II 19, a proper amount of deionized water is added into the heat collection pot 1, and the guide pipe 17 is fixed in the three-neck flask 7.
The method comprises the steps of adding a resting cell bacterium solution, a substrate, a cosolvent, a defoaming agent and the like into a three-neck flask 7, arranging a heat collection pot 1 at the bottom of the three-neck flask 7, turning on a power switch 12, simultaneously rotating a temperature adjusting knob 13 and a speed adjusting knob 14 to adjust the reaction temperature and the stirring speed, carrying out temperature monitoring through a temperature adjusting device 11, turning on an air pump 15, and adjusting the oxygen introduction amount through an air flow meter 16. Sampling is carried out at regular time in the conversion reaction process, and the conversion effect is measured.
After the conversion reaction is completed, the power switch 12 is turned off, and the temperature adjusting knob 13, the speed adjusting knob 14 and the air pump 15 are turned off. Adjusting a screw thread knob 20 of the universal clamp II 19, taking out the three-neck flask 7, and taking out the reaction liquid in the three-neck flask.
The device is suitable for fermentation and transformation of different resting cells, a simple and convenient resting cell fermentation device is designed aiming at the characteristics of simple resting cell transformation process, simple components of transformation liquid and the like, a biological reaction is carried out by utilizing the simulated fermentation tank of the heat collection pot 1, the heat collection pot 1 respectively adjusts the temperature adjusting device 11, the stirrer 5 and the air pump 15 through the temperature adjusting knob 13, the speed adjusting knob 14 and the air flow meter 16, so that the reaction temperature, the stirring speed and the oxygen introduction amount are respectively regulated and controlled, and the purposes of timely detecting and regulating and controlling the reaction process are achieved.
This device utilizes heat collection pot 1 simulation fermentation cylinder biological reaction, simple structure, and area is little to be provided with the experimental reaction parameter regulation of being convenient for of regulation and control device, have the advantage such as mix abundant, the business turn over material is convenient, fermentation effect is good, operation process is simple and convenient, washs the convenience, and low in cost has reduced testing cost, is applicable to different resting cell small-scale fermentations, the using widely of the experimental research stage of microbial fermentation of being convenient for.
Although embodiments of the present invention have been shown and described, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that changes, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principles and spirit of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. A simulated fermentation tank biological reaction device is characterized by comprising a tank body,
the heat collection pot is used for simulating the biological reaction of the fermentation tank;
the regulating device is used for regulating and controlling a power switch, the reaction temperature, the stirring speed and the oxygen introduction amount;
the iron stand is used for installing the heat collection pot and fixing the heat collection pot above the regulating device;
wherein:
the heat collection pot comprises a heater, a cooler, a stirrer, a stirring paddle and a three-neck flask which are arranged in the heat collection pot, and the stirrer, the stirring paddle and the three-neck flask are sequentially connected from top to bottom;
the regulating device comprises a bottom support and a temperature regulating device, a power switch, a temperature regulating knob and a speed regulating knob are arranged on the outer surface of the bottom support, and an air pump and an air flow meter are arranged on the right side of the bottom support;
the iron stand is arranged at the left side of the heat collecting pot and is fixedly connected with the stirrer through a universal clamp I and fixedly connected with the three-neck flask through a universal clamp II.
2. The simulated fermenter bioreactor according to claim 1, wherein the air pump is connected to the three-necked flask via a conduit, and the air flow meter is disposed on the conduit.
3. The bioreactor of claim 1, wherein a heat sink is disposed under the heat collecting pan, and a bottom support is connected under the heat sink.
4. A simulated fermentation tank bioreactor device as claimed in claim 1, characterized in that the universal clamp I and the universal clamp II are both provided with adjusting screw knobs, the stirrer is fixedly connected with the stirring paddle through the adjusting screw knobs of the universal clamp I, and the stirring paddle is fixedly connected with the three-neck flask through the adjusting screw knobs of the universal clamp II.
5. The apparatus of claim 1, wherein the paddle is connected to the three-necked flask by a stopper with a hole.
6. The simulated fermenter bioreactor of claim 1, wherein the temperature control device is a temperature controlled heater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022441824.6U CN213624180U (en) | 2020-10-28 | 2020-10-28 | Simulation fermentation cylinder biological reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022441824.6U CN213624180U (en) | 2020-10-28 | 2020-10-28 | Simulation fermentation cylinder biological reaction device |
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| Publication Number | Publication Date |
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| CN213624180U true CN213624180U (en) | 2021-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022441824.6U Active CN213624180U (en) | 2020-10-28 | 2020-10-28 | Simulation fermentation cylinder biological reaction device |
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|---|---|
| CN (1) | CN213624180U (en) |
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- 2020-10-28 CN CN202022441824.6U patent/CN213624180U/en active Active
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