CN213172349U - A transport pipe and filling device for pathogenic microorganism transports cultivation - Google Patents
A transport pipe and filling device for pathogenic microorganism transports cultivation Download PDFInfo
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- CN213172349U CN213172349U CN202021207434.6U CN202021207434U CN213172349U CN 213172349 U CN213172349 U CN 213172349U CN 202021207434 U CN202021207434 U CN 202021207434U CN 213172349 U CN213172349 U CN 213172349U
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- tube
- culture medium
- transfer
- wall
- transfer tube
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- 238000011049 filling Methods 0.000 title claims abstract description 31
- 244000000010 microbial pathogen Species 0.000 title claims abstract description 7
- 230000032258 transport Effects 0.000 title abstract description 12
- 239000001963 growth medium Substances 0.000 claims abstract description 52
- 239000007787 solid Substances 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 238000009630 liquid culture Methods 0.000 claims abstract description 10
- 244000005700 microbiome Species 0.000 claims abstract description 5
- 239000000945 filler Substances 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 6
- 238000005070 sampling Methods 0.000 claims description 5
- 239000004743 Polypropylene Substances 0.000 claims description 4
- -1 polypropylene Polymers 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 4
- 238000007789 sealing Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000006163 transport media Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000001989 nasopharynx Anatomy 0.000 description 1
- 210000003300 oropharynx Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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Abstract
The utility model provides a transport pipe for pathogenic microorganism transports cultivation, it is open that the transport pipe contains one end, and the other end is closed transports the body and is used for sealing the tube cap of open end, the inner space of transporting the body is used for holding the microorganism culture medium to and be used for the swab of sample, encircleing the surface of inner space inner wall is provided with the protruding structure of slant socle. The inner wall of the transfer pipe with the convex structure can increase the adhesion force of the solid culture medium on one hand, and on the other hand, the swab head residual liquid can be squeezed out by squeezing the swab head on the inner wall when the swab is taken out. The utility model discloses still provide the filling ware that is used for to above-mentioned transfer line filling culture medium. The filling device can form a solid-liquid two-phase culture medium in which an annular solid culture medium and a liquid culture medium coexist in the transfer pipe, and can effectively provide suitable growth and transfer conditions for various research purposes and target bacteria.
Description
Technical Field
The patent of the utility model relates to a transfer pipe and filling device belongs to the medical equipment field.
Background
The clinical and disease control scientific research field often needs to use swabs to collect microorganisms, exfoliated cells or secretions and the like, nasopharynx swabs, oropharynx swabs, anal swabs and other similar swabs and the like are common, in the detection related to bacteria, the collected swabs are often utilized to carry out pathogen isolation culture or other detection activities, if the detection is limited by environmental conditions, the field coating inoculation cannot be carried out, or the swabs need to be temporarily stored for nucleic acid detection, a swab transfer tube is often used for transferring, a conventional swab transfer tube is mostly sterile, a plastic tube without DNA or RNA pollution is contained or not contained with corresponding liquid, semisolid or solid inclined plane transfer culture media, the clinical swab samples are convenient to transfer, or the swabs can be placed in the transfer tube to transfer after inoculation, and the subsequent nucleic acid detection is convenient.
Since the pathogenic bacteria contained in the sampling swabs are different and the suitable storage conditions are different, the suitable transport medium and transport state may directly affect the subsequent detection of the samples. And most of the transfer tubes do not consider the requirement of late nucleic acid detection, and if the transfer tubes are solid or semisolid transfer culture media, later sample elution and collection can be influenced. Based on the defects existing in the prior art, the utility model aims at providing a device which can support various culture conditions and is widely suitable for the transportation pipe for collecting various pathogenic microorganism specimens and the filling of a transportation pipe culture medium.
SUMMERY OF THE UTILITY MODEL
In order to achieve the above object, the present invention firstly provides a transfer tube for transporting and culturing pathogenic microorganisms, wherein the transfer tube 1 comprises a transfer tube body 11 with one open end and a closed transfer tube cover 12 with the other end for closing the open end, the inner space 13 of the transfer tube body 11 is used for containing microorganism culture medium and a swab 14 for sampling, and the inner wall surface of the inner space 13 is surrounded by a protrusion structure 111 inclined to the bottom of the tube.
In a preferred embodiment, the distribution density of the raised structures 111 on the inner wall surface of the transfer pipe is 10 to 100/cm2. In a preferred embodiment, the distribution density of the protruding structures 111 is 60/cm2。
In a more preferred embodiment, the height of the protruding structure 111 protruding from the tube wall is 0.05-0.2 cm. In a preferred embodiment, the height of the raised structure 111 protruding from the wall of the tube is 0.1 cm.
More preferably, the three-dimensional structure of the convex structure 111 is a cone with a cone tip slightly inclined towards the tube bottom.
More preferably, the material of the transfer tube 11 and the protruding structure 111 are both polypropylene or plastic tube of similar material.
Preferably, a solid culture medium 112 is laid on the surrounding inner wall of the inner space 13, the height of the solid culture medium 112 is about 1/3-2/3 of the whole height of the transfer tube, and a liquid culture medium 131 is placed at the bottom in the inner space 13.
Still preferably, the liquid culture medium is submerged at a height of 1/3-2/3 of the solid culture medium.
The utility model provides a transfer pipe is covered with small protruding structure to the bottom of a tube slope at the inner wall. This scheme will be transported the intraductal wall preparation area and small to the protruding effect of tube bottom slope, use side opening filling ware to make solid medium solidify around the transfer pipe at the transfer pipe system in-process of irritating, form hollow cyclic annular after extracting the filling ware, can pour into a certain amount of liquid medium into to the hollow region through filling ware inlayer pipe, form solid-liquid two-phase transport medium. According to the technical scheme, on one hand, the adhesion force can be increased when the solid culture medium is attached to the inner wall, on the other hand, before the swab is taken out, the swab can be rubbed and extruded on the inner wall, and the tiny convex structure inclined towards the bottom of the tube is helpful for squeezing out liquid and possible samples carried by the swab head. Various swabs of sampling can be broken and placed in the transfer pipe after appropriate length, also can refer to other transfer pipes and will transfer the form that the pipe cover design for can fix the disconnected section, be convenient for transport and take out the swab.
Secondly, in order to complete the filling of the solid-liquid two-phase transfer culture medium in the transfer pipe, the utility model also provides a filling device design, the filling device 2 is arranged into a cylinder structure with the diameter smaller than the inner diameter of the transfer pipe, a hollow inner tube 51 is arranged at the circle center part of the cross section of the filling device, the length of the inner tube 51 penetrates through the whole filling device 2, an upper opening 511 and a lower opening 512 are arranged, an outer sleeve 52 surrounding the inner layer tube 51 is arranged on the periphery of the inner layer tube 51, the outer sleeve is closed at 1/3-2/3 height from top to bottom of the whole filling device (the opening height of the outer sleeve is determined by the height of a solid culture medium surrounding the inner wall, and is slightly lower than the height of the solid culture medium, so that the solid culture medium which is not solidified after high-temperature sterilization can flow out from the side opening), and a side opening 53 which is laterally opened to the outside is arranged at the closed end.
More preferably, the number of the side openings 53 is 2 to 16.
It is particularly preferred that the number of the side openings 53 is 4.
The using method of the filling device comprises the steps of inserting the cylindrical filling device into the idle conveying pipe, enabling the diameter of the filling device to be smaller than the inner diameter of the conveying pipe, injecting the prepared solid culture medium into the upper sleeve pipe, enabling the culture medium to flow out of the side opening of the filling device and flow into the conveying pipe, slightly rotating the filling device after the culture medium is solidified, pulling out the filling device to form the annular solid culture medium, simultaneously injecting the liquid culture medium into the hollow part of the annular solid culture medium through the hollow inner layer pipe to form the solid-liquid two-phase culture medium, and controlling the height of the injected liquid to be 1/3-2/3 which is used for immersing the solid culture medium, wherein the height of the injected liquid is not larger than the height of the solid.
The utility model discloses a filling ware is accomplished the filling to transport pipe solid-liquid two-phase culture medium, is convenient for select different solid and liquid culture medium combinations according to actual need in the work, can effectually provide suitable growth and transportation condition for various research purposes and target fungus. The annular solid culture medium and the liquid culture medium form a solid-liquid two-phase culture medium, so that on one hand, conditions are provided for the growth of target bacteria as much as possible, and the condition that the surface of the solid culture medium is completely covered by liquid possibly caused by the inclination of the transfer culture tube, and further the separation culture of the target bacteria is influenced is avoided. On the other hand, the liquid culture medium without DNA-RNA pollution can be used as an eluent in sample extraction to provide sufficient buffer conditions for sample nucleic acid extraction, and nucleic acid detection can directly absorb part or all of the liquid culture medium for subsequent nucleic acid extraction and detection to avoid secondary elution of a swab.
Drawings
FIG. 1 is a longitudinal cross-sectional view of a transfer tube;
FIG. 2 is an enlarged view of the inner wall of the transfer tube;
fig. 3 is a perspective view of the pourer.
Detailed Description
The invention will be further described with reference to specific embodiments, the advantages and features of the invention will become more apparent as the description proceeds. These examples are exemplary only and do not limit the scope of the invention as defined in the claims.
EXAMPLE 1 preferred design of transfer pipe
The accompanying drawings 1 and 2 show a preferred embodiment of the transfer tube of the present invention, as shown in fig. 1, in which the transfer tube for transferring and culturing pathogenic microorganisms is a plastic tube 11 with one open end and the other closed end, a plastic cap 12 for closing the open end, the cap and the open end can be in a screw type connection, or in a clip type connectionAnd (4) buckling connection. The inner space 13 of the polypropylene or similar plastic pipe is used for containing a microorganism culture medium and a swab 14 for sampling, a convex structure 111 inclined to the bottom of the pipe is arranged on the surface surrounding the inner wall of the inner space 13, and the distribution density of the convex structure 111 is 60/cm2The height of the protruding structure 111 protruding from the tube wall is 0.1 cm. The spatial structure of protruding structure 111 is the cone that the tip slightly leaned to the socle, the material of transporting body 11 and protruding structure 111 is polypropylene or similar material plastic tubing. Solid medium 112 is laid in a raised structure around the inner wall and liquid medium 131 is placed in the inner space 13.
Example 2 preferred design of the filling element
Fig. 3 shows a preferred embodiment of the present invention, in which the filler 2 is configured as a cylinder with a diameter smaller than the inner diameter of the transfer tube, the circle center of the cross section of the filler is partially provided with a hollow inner tube 51, the length of the inner tube 51 runs through the entire filler 2, and is provided with an upper opening 511 and a lower opening 512, the outer circumference of the inner tube 51 is provided with an outer sleeve 52 surrounding the inner tube 51, the outer sleeve 52 is highly closed at 1/2 from the top to the bottom of the entire filler, and the closed end is provided with a side opening 53 leading to the outside in the lateral direction. The number of the side openings 53 is 4 (only 2 are represented in the figure).
Example 3 method of perfusing culture Medium into transport tubes Using a perfusion apparatus
The using method of the filling device comprises the following steps: the cylinder filling device 2 is inserted into an empty transfer pipe, the diameter of the filling device is smaller than the inner diameter of the transfer pipe, after a prepared solid culture medium is injected from the upper part of a hollow outer sleeve 52 pipeline, the culture medium flows out from side openings 53 on the periphery of the filling device and flows into the transfer pipe, after the culture medium is solidified, the filling device is slightly rotated and pulled out to form an annular solid culture medium, a liquid culture medium is injected into the hollow part of the annular solid culture medium through an inner layer pipe 51, a solid-liquid two-phase culture medium can be formed, the injected liquid is controlled to be 1/2 degrees for immersing the solid culture medium, and the height of the solid culture medium cannot be exceeded.
Claims (10)
1. A transfer tube for pathogenic microorganism transfer culture, the transfer tube (1) comprises a transfer tube body (11) with one open end and the other closed end and a tube cover (12) for closing the open end, an inner space (13) of the transfer tube body (11) is used for containing microorganism culture medium and a swab (14) for sampling, and is characterized in that a convex structure (111) inclined to the bottom of the tube is arranged on the surface surrounding the inner wall of the inner space (13).
2. The transfer tube according to claim 1, wherein the distribution density of the raised structures (111) on the inner wall surface of the transfer tube is 10-100/cm2。
3. The transfer tube according to claim 2, wherein the height of the protruding structure (111) protruding from the tube wall is 0.05-0.2 cm.
4. A transfer pipe according to claim 3, wherein the relief structure of the raised structure (111) is a cone with a cone tip slightly inclined towards the bottom of the pipe.
5. Transfer tube according to claim 4, characterized in that the transfer tube body (11) and the raised structure (111) are both of polypropylene.
6. Transfer pipe according to claim 5, characterized in that a solid culture medium (112) is laid on the surrounding inner wall of the inner space (13), the height of the solid culture medium (112) being about 1/3-2/3 of the overall height of the transfer pipe, and that a liquid culture medium (131) is placed at the bottom in the inner space (13).
7. The transfer tube of claim 6, wherein the liquid medium submerges the solid medium to a height of 1/3-2/3.
8. A filler for filling a carrier tube with a culture medium according to any one of claims 1 to 7, wherein the filler (2) is provided in a cylindrical structure having a diameter smaller than the inner diameter of the carrier tube, a hollow inner tube (51) is provided at a center portion of the cross section of the filler, the inner tube (51) has a length extending through the entire filler (2), an upper opening (511) and a lower opening (512) are provided, an outer tube (52) surrounding the inner tube (51) is provided at the outer periphery of the inner tube (51), the outer tube (52) is closed at a height of 1/3 to 2/3 from the top to the bottom of the entire filler, and a side opening (53) is provided at the closed end, the side opening leading to the outside.
9. The pourer of claim 8, wherein the number of side openings (53) is 2-16.
10. A filler according to claim 9, characterised in that the number of side openings (53) is 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021207434.6U CN213172349U (en) | 2020-06-25 | 2020-06-25 | A transport pipe and filling device for pathogenic microorganism transports cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021207434.6U CN213172349U (en) | 2020-06-25 | 2020-06-25 | A transport pipe and filling device for pathogenic microorganism transports cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213172349U true CN213172349U (en) | 2021-05-11 |
Family
ID=75789606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021207434.6U Active CN213172349U (en) | 2020-06-25 | 2020-06-25 | A transport pipe and filling device for pathogenic microorganism transports cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213172349U (en) |
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2020
- 2020-06-25 CN CN202021207434.6U patent/CN213172349U/en active Active
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240227 Address after: 102200 1101, building 3, No.1 a, Chaoqian Road, science and Technology Park, Changping District, Beijing Patentee after: Beijing Jinyuan Liheng Biotechnology Co.,Ltd. Country or region after: China Address before: No.155 Changbai Road, Changping District, Beijing 102206 Patentee before: NATIONAL INSTITUTE FOR COMMUNICABLE DISEASE CONTROL AND PREVENTION, CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION Country or region before: China Patentee before: SHENZHEN NANSHAN CENTER FOR DISEASE CONTROL AND PREVENTION |
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| TR01 | Transfer of patent right |