CN212589712U - Biological observation device for breeding life of overwintering imagoes of castanea mollissima - Google Patents

Abstract

The utility model belongs to the technical field of insect research equipment, specifically a castanea mollissima overwintering adult life-span biological observation device. Including raising the dish and setting up the test tube of raising on raising the dish, raising the dish and including the peaceful chassis apron of pan, the pan top is provided with the pan apron, is provided with the test tube jack that a plurality of groups evenly set up on the pan apron, raising the test tube and inserting and establish in the test tube jack, raising the test tube and be both ends open-ended piping structure, raising the test tube both ends and having blocked up the stopper respectively, wherein raise the stopper of test tube upper end and raise and be provided with the leaf section between the test tube inner wall, the closed section that the stopper was extended to leaf section upper and lower end. The utility model discloses reduce experimental land used, reduced the expense of hiring a land, reduced intensity of labour, improved experimental condition.

Description

Biological observation device for breeding life of overwintering imagoes of castanea mollissima

Technical Field

The utility model belongs to the technical field of insect research equipment, specifically a castanea mollissima overwintering adult life-span biological observation device.

Background

The Sedum castanea (Oulema tristis Herbst), also known as Diagnostica graminis, belongs to the order Coleoptera (Coleoptera) and Diagnostica. Flaccid beetles are primarily harmful to millet, but also to millet, wheat, corn and various grassy weeds. The harm of millet and broomcorn millet has been recorded in the fifties of the last century, but the harm happens sporadically all the time, and the harm is gradually increased year by year from the beginning of the century and gradually increased to main pests of millet and broomcorn millet. The castanea mollissima adults climb out from soil seams after overwintering, and are fully fed with leaves of millet and the like to be copulated and lay eggs, wherein eggs are scattered on the back surfaces of the 1 st to 6 th leaves of the grain seedlings and are individually laid on the front surfaces of the leaves. In order to accurately grasp the biological characteristics of the wintering imagoes of the setaria italica, artificial feeding and observation are needed.

In the prior technical scheme, millet is sowed about 10 days in 4 months, a small arched shed is built on the millet, and an insect-proof net is covered on the millet, so that the setaria italica is prevented from laying eggs on the millet seedlings. Thinning and final singling according to the plant spacing of 5 cm and the row spacing of 10 cm. Inserting a bamboo pole beside the grain seedling, binding 4 pieces of bamboo pole from the top with thin iron wire, tightening with pliers, like inserting a tomato pole, tying a thin iron wire on the bamboo pole about 40-50 cm away from the ground, and bending the iron wire into a hook with pliers. Sawing a circle by using a steel saw blade at a position 1 cm away from the inner side of the thick end of the wooden plug to form a groove with the depth of about 0.1 cm, wrapping cotton on the wooden plug with the thickness of about 0.15 cm, wrapping the cotton by using a plastic film, and fastening the cotton by using a string from the groove. The mineral water bottle is cut off by scissors from the middle, the cut is removed and left, the cut is carefully trimmed without leaving stubble, an insect-proof net is covered on the cut and is tied by a rubber band, and a hole is tied on the bottle body by a sewing needle according to a 1 cm square to facilitate ventilation. The cover of the mineral water bottle is screwed off, a section of the string with the length of about 40 cm is taken, one end of the string is tied on the bottle mouth, and the other end of the string is knotted to form a ring structure. Taking male and female paired castanea mollissima adults and putting the castanea mollissima adults in a bottle in a pair, quickly taking a grain seedling leaf without eggs on the upper surface, extending about 8 centimeters from a bottle opening, and tightly plugging the grain seedling leaf with a plug (the leaf is tightly attached to the wall of the bottle opening). The rope is hung on the hook, so that the millet leaves are kept to be inclined upwards by about 45 degrees.

The blades are cut off by scissors from the position close to the bottle mouth at the same time every day, the whole insect breeding bottle is taken back indoors, the plug is pulled out, the millet blades are taken out by tweezers and are lightly placed on a piece of white paper, the bottle cover is screwed on to prevent adults from escaping, sometimes the adults put back the adult bottles along with the coming-out of the blades immediately, and then the cover is screwed on. Counting the eggs on the membranes on the upper part of the leaf and the inner end of the plug, observing whether the inner wall of the bottle and the inner surface of the insect-proof net are egg-stuck or not from the outside of the bottle, if so, taking off the insect-proof net, temporarily placing the adult in an empty bottle, wiping off the eggs while counting, then placing the adult back, and covering and tightening the insect-proof net. The number of eggs at each location was collected and used as the number of eggs laid by the pair of adults on the day. Screwing off the bottle cover, replacing the plug and plugging the bottle opening, bringing the bottle opening to the side of outdoor rice seedlings, selecting a non-egg blade (cutting off a section of blade, if the length is more than 15 cm, the blade can be used), shaking the adult to the bottle bottom (on an insect-proof net), pulling out the plug, inserting the blade, plugging the plug, hanging a hook, and completing the egg counting work on the day. And finishing egg counting work after the female adults die, and finishing the life observation of the adults after the female adults and the male adults die. The relationship between the egg laying amount and the life of the adult insects and the temperature, the humidity, the rainfall and the like is analyzed by utilizing the data of the temperature, the humidity, the rainfall and the like of the local meteorological bureau day by day.

The prior art has the following problems:

1. the bamboo pole and the feeding bottle can shield sunlight and influence the illumination conditions of the seedlings such as the millet and the like. 2. The test land is more, and the renting cost is higher. 3. The egg laying surface is large, and the egg counting time is long. 4. The adult castanea mollissima cannot be bred in an artificial climate box. 5. When the leaf blade is inserted between the outdoor millet seedling rows, the adult castanea mollissima still has the opportunity of escaping. 6. The labor intensity is high, and the test operation conditions are poor. 7. The test operation time is longer.

The problems of influence on illumination of grain seedlings, large test land, high land renting cost, long test operation time, incapability of feeding in an artificial climate box, escape of adults, higher labor intensity, poor test operation condition and the like exist in the feeding and observation of the prior art.

Disclosure of Invention

The utility model provides a solve the above problem, provide a maize leaf beetle adult life-span biology observation device that winters.

The utility model adopts the following technical proposal: the utility model provides a castanea mollissima adult life-span biology observation device that breeds, is including raising the dish and setting up raising the test tube on raising the dish, raising the dish and including the flat chassis apron of pan, the pan top is provided with the pan apron, is provided with the test tube jack that a plurality of groups evenly set up on the pan apron, raising the test tube insert and establish in the test tube jack, raising the test tube and be both ends open-ended pipe dress structure, raising the test tube both ends and having blocked up the stopper respectively, wherein raise the stopper of test tube upper end and be provided with the leaf section between the test tube inner wall, the closed section of stopper is extended to leaf section upper and lower end.

Furthermore, an opening at the upper end of the feeding test tube is sequentially provided with wet cotton cloth and a preservative film from inside to outside, and the wet cotton cloth and the preservative film are bound through rubber bands.

Furthermore, a pull rope is arranged outside the plug.

Further, a light source is arranged at the bottom of the flat tray.

Furthermore, a water inlet is formed in the middle of the side wall of the flat base, and a water outlet is formed in the bottom of the side wall of the flat base.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. has no shelter to seedlings such as millet and the like.

According to the prior art, bamboo poles are inserted between grain rows, feeding bottles are arranged on seedlings, and the bamboo poles and the feeding bottles have the defect of shading the grain seedlings, so that the growth and development of the grain seedlings are influenced.

According to the scheme, no other objects exist between the rows of seedlings such as the millet and the like, the shielding influence of the other objects on the seedlings such as the millet and the like is avoided, and the seedlings such as the millet and the like are sufficiently illuminated.

2. The land for test is reduced, and the land renting cost is reduced.

In the prior art, because bamboo poles, feeding bottles and the like are arranged between grain seedling lines to shade, the operation of replacing blades is required to be carried out between the lines every day, the row spacing of the seedlings such as millet is at least 10 cm, and the plant spacing is at least 5 cm; the leaves of the feeding bottle are extended by about 8 cm every day, and the whole section is the whole section regardless of the width.

In the scheme, no shielding object is arranged between the rows of seedlings such as millet and the like, the illumination is sufficient, the operation of replacing blades is not needed between the rows, the planting distance can be reduced to 2.5 cm under the condition that the row distance is not changed, the seedlings with the same number are cultivated, and the test land is only reduced by half in the next item, compared with the scheme; the length of the leaf blade extending into the test tube every day is 6 cm, the width is about 0.5 cm, the whole leaf blade is narrow, if the width of the leaf blade exceeds 1 cm, the leaf blade can be divided into 2 parts or even 3 parts, the leaf blade 1/2 to 2/3 can be saved in the link compared with the first scheme, and the test land can be correspondingly reduced to 1/2 to 2/3.

3. The occupied space for feeding is reduced.

The feeding bottle volume for feeding 1 pair of castanea mollissima in the prior art is about 190 milliliters.

The test tube volume of the feeding 1 to the castanea mollissima =0.75 × 3.14 × 15=26.5 (milliliters), the feeding space is 14% of the first scheme, and the feeding space is obviously reduced.

4. Can be raised in a climatic chamber.

In the prior art, a pair of chestnut leafbeetles is bred by using 4 bamboo poles with the length of 100 centimeters and must be bred by using leaves on seedlings, so the pair of chestnut leafbeetles cannot be bred in an artificial climate box.

The scheme does not use bamboo poles, the feeding occupied space is small, the occupied space of the feeding 80 to the castanea mollissima is approximately equal to 80 x 26.5=2119.5 (cubic centimeter), one end of the leaf segment is inserted into water, the cut millet and other leaves can be kept fresh for 72 hours, the leaves are changed once a day, the requirements of the castanea mollissima on feeds can be completely met, and the castanea mollissima can be fed in an artificial climate box.

5. The escape opportunity of the adult is reduced, and the condition that data are wasted due to the escape of the adult is reduced.

In the prior art, the leaf replacement is carried out outdoors, the castanea mollissima accidentally runs out of the insect breeding device and generally flies shadowless, and the test data of the castanea mollissima is wasted.

With this scheme, the whole process of trading test tube, stopper, leaf section, statistics ovum number is indoor, and the adult runs out the insect feeding device by accident, can catch, put back very conveniently, has obviously reduced the chance that the adult escaped, has reduced and has caused the condition emergence that data was wasted because of the adult escapes.

6. The labor intensity is reduced, and the test conditions are improved.

According to the prior art, the leaves need to be replaced outdoors, the seedlings squat for a long time between rows, and after the leaf replacement operation is completed, waist soreness and backache are often caused, and meanwhile, the seedlings are bitter in sunshine and rain.

By the scheme, the whole process of changing the test tube, the plug, the leaf segment and counting the number of eggs is indoors, the time for collecting the leaves outdoors is about 1/20 for changing the leaves in the scheme I, the labor intensity is obviously reduced, the working condition is obviously improved, and the time for sunning and raining is obviously shortened.

7. The test operation time is reduced.

The blade, the test tube and the plug are convenient and time-saving to replace. The blade section is changed from outdoor field operation of the scheme I to indoor operation on an indoor operating platform, so that the operation is more convenient and comfortable, and the time is saved; the operation of changing the test tube and the plug is also more convenient and faster than the case. Egg counting time is reduced. The feeding tube internal surface area is about 41% of the internal surface area of the feeding bottle in the first scheme (1.5 x 3.14 x (15-1.5 x 2) +0.75 x 3.14 x 2)/(190/2 +4 x 3.14+0.8 x 3.14) ≈ 41%), the cut diameter of the feeding bottle in the first scheme is about 8 cm, the diameter of the cut opening is about 1.6 cm), the egg laying area of the castanea mollissima adults is correspondingly reduced by 41%, and eggs are more convenient to count and the egg counting time is reduced compared with the first scheme.

In 2019, a test of the influence of different feeds on the reproductive life of the castanea mollissima adults is carried out, 4 treatments (feeding by millet, tare, green bristlegrass herb and digitaria sanguinalis) are carried out, each treatment is carried out for 3 times, 5 pairs of adults are carried out, 60 pairs of the castanea mollissima adults are fed and observed by the scheme, 1.5 hours are needed for changing test tubes, plugs, leaf segments and eggs every day, if the scheme is about 2 hours, the reproductive life of the castanea mollissima adults is fed and observed by the scheme, and the test operation time can be saved by 0.5 minute for each pair on average.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of a feeding tray;

FIG. 3 is a top view of the feeding tray;

FIG. 4 is a schematic view of the structure of a feeding tube;

in the figure, 1-a flat-bottom plate, 2-a flat-bottom plate cover plate, 3-a water inlet, 4-a water outlet, 5-a feeding test tube, 6-a rubber band, 7-wet cotton cloth, 8-a preservative film, 9-a test tube jack, 10-a circular table, 11-a plug, 12-a pull rope, 13-a castanea mollissima adult, 14-a leaf segment and 15-a light source.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in figures 1-4, a castanea mollissima adult life-span biological observation device that breeds that overwintering, including raising the dish and setting up raising the test tube 5 on raising the dish, raising the dish and including flat base 1 and flat chassis apron 2, 1 lateral wall middle part of flat base is provided with water inlet 3, 1 lateral wall bottom of flat base is provided with delivery port 4, 1 top of flat base is provided with flat base apron 2, is provided with the test tube jack 9 that a plurality of groups evenly set up on flat base apron 2, raising test tube 5 insert and establish in test tube jack 9, raising test tube 5 and being both ends open-ended piping structure, raising test tube 5 both ends and having blocked up respectively stopper 11, wherein raise and be provided with between the stopper of test tube 5 upper end and raise the test tube 5 inner wall and have leaf section 14, the closed section of stopper 11 is extended to leaf section 14 upper. Raise 5 upper end openings of test tube and have set gradually wet cotton 7 and plastic wrap 8 from inside to outside, wet cotton 7 and plastic wrap 8 pass through the rubber band ligature. A pull rope 12 is arranged outside the plug 11. The bottom of the flat base 1 is provided with a light source 15. The light source 15 is connected to an external power source.

Seeding millet, tare, green bristlegrass, crab grass and other castanea mollissima feed plants about 4 months and 10 days, building a small arched shed on the seed, and covering an insect-proof net to prevent the castanea mollissima from laying eggs on seedlings. Thinning and final singling at plant spacing of 2.5 cm and row spacing of 10 cm.

One end of the feeding test tube is plugged and sealed by a plug, and the outer plane of the plug is flush with the mouth of the feeding test tube; taking the castanea mollissima adults unearthed in winter, putting the castanea mollissima adults into the test tubes in a male-female pairing mode, quickly plugging the other ends of the test tubes by plugs, writing the serial numbers of the castanea mollissima adults (the end of each test tube is hereinafter called a serial number end) at one end of each test tube by an oil marker, inserting the test tubes into test tube inserting holes in a flat-bottom plate cover plate, enabling the serial number ends to face downwards, switching on a power supply of a light source below the flat-bottom plate, enabling the serial number ends to face towards the light source, and generally climbing towards one end of the light source, wherein the test tubes are called feeding test tubes as shown in figure.

When the same-processed and same-repeated castanosoma pomona imago is completely put into a feeding test tube, leaf segments are inserted for feeding the imagoes. Cutting leaves from seedlings such as millet and the like, cutting and processing the cut leaves into leaf segments according to the specification of about 6 cm in length and about 0.5 cm in width, taking the feeding test tube from a test tube jack by the left hand, if the chestnut leaf shell is not at a numbering end, vertically feeding the test tube with the numbering end facing downwards, flicking the test tube wall by using the fingers of the right hand, dropping the chestnut leaf shell to the numbering end, taking a leaf segment with the top end facing to the end (hereinafter referred to as a feed end) which is attached to the outer wall of the test tube along the direction of the test tube in the test tube, pinching the plug and the millet leaf segment together by using the left hand, enabling the bottom end of the leaf segment to be longer than the test tube opening by about 1.5 cm, pinching the pull rope on the plug of the feed end by the right hand to pull outwards to pull out about 0.3 cm, pinching the plug and the millet leaf segment simultaneously by using the fingers to pull the plug outwards, and plugging the leaf segment simultaneously when the plug is pulled out of the test tube, enabling the, the leaf section is tightly attached to the inner wall of the test tube, and the outer end of the leaf section is flush with the opening of the test tube.

All with handling after all inserting the leaf section with repeated test tube, the power of the light source below the disconnection pan, the fodder end up, cover on raising the test tube after the cotton dips in water, the plastic wrap covers on the cotton again, reduces the evaporation of water, ties up plastic wrap, cotton on raising the test tube with the rubber band, and the serial number end is downward, inserts in the test tube jack on the pan apron, puts into indoor or the artificial climate incasement. Feeding, mating and laying eggs of the wintering adults of the setaria italica in a feeding test tube.

And replacing the test tube, the plug and the leaf segment on the next day, and simultaneously recording the number of spawns and the survival condition of adults. The preservative film and the cotton cloth on the feeding test tube are removed, the feeding test tube is turned over, the feed end is downwards inserted into the test tube hole again, one feeding test tube is inserted into one hole, the cleaned test tube, the plug and the new leaf section are placed on the operating table, the plug is plugged into one end of the test tube, the empty test tube with the same number and the feeding test tube are inserted into the test tube hole in a pairwise manner, a power supply of a light source below the flat base plate is switched on, the phototaxis of the castanea mollissima adults is utilized, the castanea mollissima adults generally climb to the feed end, the left hand takes up the feeding test tube for observation, if the castanea mollissima is not at the feed end, the feed end downwards flicks the tube wall by fingers, the castanea mollissima can fall to the feed end, the left hand is used for simultaneously pinching the. Hold between the fingers the stay cord with the hand and extract the stopper of raising test tube number end, stand upside down rapidly, meet the alignment with empty test tube opening one end, hold between the two test tubes simultaneously with the left hand at two test tube kneck, the test tube outer wall of chestnut leaf beetle is raised to right hand finger flick, when a pair of chestnut leaf beetle is whole to fall to empty test tube, plug the test tube mouth with clean stopper rapidly, in time plug back the stopper of just pulling out to original raising on the pipe again, when the same treatment with the repetition chestnut leaf beetle is whole to be changed to clean test tube after, change the new leaf section with aforementioned method, feed end damping cloth, the plastic wrap, tie tightly on raising the pipe with the rubber band, the number end is inserted down in the test tube jack, the power of the light source below the flat-bottom dish is disconnected, put into indoor or artificial climate incasement, raise the observation one day next time.

Observing eggs on the inner wall of the feeding pipe, the inner surface of the plug and the front and back surfaces of the leaf section which are replaced on the previous day one by one, and counting. The test tube, the plug and the millet leaf segment are changed every day, the egg laying number is recorded, and the survival condition of the castanea mollissima adults is observed until the adults die.

When eggs laid in the day are laid, the subsequent tests such as measurement of the sizes of the eggs and incubation and development of the eggs can be carried out, after the eggs are all incubated, the rice leaf sections are placed in the field for composting, and the test tubes and the plugs are washed with clear water and dried in the air, so that the rice leaf sections can be repeatedly used.

1. The apex direction of blades such as millet in this scheme calls the top, and the bottom is called to leaf sheath direction, because there is the fine hair towards the top direction above the blade, and the top will be towards in the test tube when filling in the test tube, otherwise, the blade stretches into the test tube overlength along with the stopper easily, nevertheless is difficult to the adjustment and extracts.

2. Preferably, no more than one leaf blade is cut from the same millet seedling every day, and the collection is performed alternately, so that the influence on the growth and development of the millet seedlings is reduced.

3. At least two rubber bands are used for fastening the preservative film on the feeding pipe, so that the phenomenon that wet cotton cloth and the preservative film fall off, leaf sections wither and wither, food supply of the leaf beetles is influenced, and propagation and survival of the adult chestnut beetles are influenced due to the fact that the rubber bands age and break is prevented.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims (5)

1. A castanea mollissima overwintering adult reproduction life biological observation device is characterized in that: including raising the dish and setting up raising test tube (5) on raising the dish, raising the dish and including flat base plate (1) and flat chassis apron (2), flat base plate (1) top is provided with flat base plate apron (2), is provided with test tube jack (9) that a plurality of groups evenly set up on flat base plate apron (2), raising test tube (5) insert and establish in test tube jack (9), raising test tube (5) and be both ends open-ended piping structure, raising test tube (5) both ends and stifled respectively and have stopper (11), wherein raise the stopper of test tube (5) upper end and raise and be provided with between test tube (5) the inner wall leaf section (14), the closed section of stopper (11) is extended to the lower extreme in leaf section (14).

2. The device for biologically observing the reproductive life of an adult wintering beetle according to claim 1, comprising: raise test tube (5) upper end opening from inside to outside and set gradually wet cotton (7) and plastic wrap (8), wet cotton (7) and plastic wrap (8) are through the rubber band ligature.

3. The device for biologically observing the reproductive life of an adult wintering beetle according to claim 1, comprising: and a pull rope (12) is arranged on the outer side of the plug (11).

4. The device for biologically observing the reproductive life of an adult wintering beetle according to claim 1, comprising: the bottom of the flat-bottom plate (1) is provided with a light source (15).

5. The device for biologically observing the reproductive life of an adult wintering beetle according to claim 1, comprising: the water inlet (3) is arranged in the middle of the side wall of the flat base (1), and the water outlet (4) is arranged at the bottom of the side wall of the flat base (1).

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