CN210534176U - Reagent package device for full-automatic chemiluminescence immunoassay - Google Patents
Reagent package device for full-automatic chemiluminescence immunoassay Download PDFInfo
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- CN210534176U CN210534176U CN201921361514.4U CN201921361514U CN210534176U CN 210534176 U CN210534176 U CN 210534176U CN 201921361514 U CN201921361514 U CN 201921361514U CN 210534176 U CN210534176 U CN 210534176U
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 358
- 238000003018 immunoassay Methods 0.000 title claims abstract description 21
- 238000001125 extrusion Methods 0.000 claims abstract description 49
- 230000005540 biological transmission Effects 0.000 claims abstract description 10
- 239000012528 membrane Substances 0.000 claims description 19
- 238000001514 detection method Methods 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 5
- 238000005192 partition Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 10
- 238000007664 blowing Methods 0.000 description 9
- 238000000034 method Methods 0.000 description 7
- 238000009434 installation Methods 0.000 description 6
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- 238000010521 absorption reaction Methods 0.000 description 4
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- 230000007547 defect Effects 0.000 description 2
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- 239000000758 substrate Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
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- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
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- 238000003127 radioimmunoassay Methods 0.000 description 1
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Abstract
The utility model discloses a reagent package device for full-automatic chemiluminescence immunoassay, including the reagent cup, reagent cup carousel and reagent extrusion mechanism, the inside partition of reagent cup becomes a plurality of reagent chambeies, be provided with a plurality of reagent outlet pipes on the outside circumference of reagent cup, a reagent chamber sets a reagent outlet pipe, reagent outlet pipe and reagent chamber intercommunication, reagent cup carousel is connected on the reagent cup, be provided with a plurality of through-holes and a transmission groove on the reagent carousel, the quantity of through-hole is the same with the reagent chamber, every through-hole communicates with a reagent chamber respectively, the transmission groove cup joints on the output shaft of motor, the motor drives the rotation of reagent cup carousel. The utility model discloses replace artifical reagent of absorbing, improved reagent and absorb efficiency, make reagent package structure compacter, can be applied to full-automatic chemiluminescence immunoassay device.
Description
Technical Field
The utility model relates to a biochemical analysis device especially relates to an utilize chemiluminescence method to carry out immunoassay's instrument.
Background
ChemiLuminescence (CL) is a type of molecular luminescence spectroscopy, and is a trace analysis method for determining the content of an analyte by detecting the ChemiLuminescence intensity of a system by using an instrument according to the principle that the concentration of the analyte in a chemical detection system and the ChemiLuminescence intensity of the system are in a linear quantitative relationship under a certain condition. The chemiluminescence method has wide application in the fields of trace metal ions, various inorganic compounds, organic compound analysis and biology.
Chemiluminescence immunoassay (CLIA) is a technique for detecting and analyzing various antigens, antibodies, hormones, enzymes, vitamins, drugs and the like by combining a chemiluminescence assay technique with high sensitivity and a high-specificity immunoreaction. Is an immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluoroimmunoassay and time-resolved fluoroimmunoassay. The chemiluminescence method has the advantages of high sensitivity, strong specificity, high accuracy, wide detection range and the like. Compared with the semi-quantitative enzyme-linked immunosorbent assay, the chemiluminescence assay is real quantitative, and the detection speed is higher and more convenient. Meanwhile, the chemiluminescent marker is stable, the effective period of the reagent is long, and the requirement of clinical application is greatly facilitated.
When carrying out chemiluminescence and detecting, reagent is essential material (such as washing liquid, reactant, luminous substrate etc.), when what reagent was required during current operation, the operator took the instrument to absorb the reagent and pours into the capillary with the absorption, and the biggest defect that such operation exists is need absorb many times, and operating efficiency is low, needs the artifical relevant reagent of incessant absorption, and intensity of labour is big. And is not suitable for a full-automatic chemiluminescence immunoassay device.
SUMMERY OF THE UTILITY MODEL
In order to overcome the operating efficiency low that above-mentioned reagent manual absorption exists, the great defect of intensity of labour, the utility model provides a reagent package device for full-automatic chemiluminescence immunoassay replaces the manual work to absorb reagent through the device, provides reagent absorption efficiency, can be applied to full-automatic chemiluminescence immunoassay device.
In order to solve the technical problem, the utility model discloses the technical scheme who adopts is:
a reagent package device for full-automatic chemiluminescence immunoassay is characterized in that: the reagent cup comprises a reagent cup, a reagent cup turntable and a reagent extrusion mechanism, wherein the inside of the reagent cup is divided into a plurality of reagent cavities, a plurality of reagent outlet pipes are arranged on the outer circumference of the reagent cup, one reagent cavity is provided with one reagent outlet pipe, the reagent outlet pipe is communicated with the reagent cavities, the reagent cup turntable is connected onto the reagent cup, a rubber film is arranged at the joint of the reagent turntable and the reagent cup and used for sealing the reagent cavities (ensuring that the reagent can only be extruded out of the reagent outlet pipe and can not flow out of the other end of the reagent cavity), the reagent turntable is provided with a plurality of through holes and a transmission groove, the number of the through holes is the same as that of the reagent cavities, each through hole is respectively communicated with one reagent cavity, the transmission groove is sleeved on an output shaft of a motor, the motor drives the reagent cup turntable to rotate, the reagent extrusion mechanism comprises an extrusion rod and a power source, and the extrusion rod, Oil cylinder, electric push rod, etc.) and under the action of a power source, the extrusion rod extends into the extrusion from the through hole and extrudes on the rubber membrane, and the reagent in the reagent cavity is extruded through the deformation of the rubber membrane, so that the reagent is extruded from the reagent outlet pipe. The extrusion rod is pushed to extrude the rubber membrane, and the reagent in the reagent cavity is extruded out through the deformation of the rubber membrane. Because a plurality of reagent cavities are arranged in the reagent cup, one reagent is stored in each reagent cavity, when the reagent needs to be extruded, the rotating motor rotates, the reagent cavity with the reagent is aligned to the extrusion rod, and then the extrusion rod acts to extrude the reagent. In order to accurately position whether the required reagent chamber is aligned with the extrusion rod, a sensor can be arranged at the extrusion rod to detect whether the reagent chamber is aligned with the extrusion rod.
In order to facilitate the adjustment of the distance between the reagent pack device and the turntable assembly and facilitate capillary siphon reagent, the reagent pack device further comprises a reagent mounting seat, a reagent line rail and a reagent motor, a reagent gear is sleeved on an output shaft of the reagent motor, a reagent rack is mounted on the reagent mounting seat and meshed with the reagent rack, and the reagent mounting seat is mounted on the reagent line rail and slides on the reagent line rail under the action of the reagent motor. The reagent turntable, the rotating motor and the power source are all installed on the reagent installation seat.
In order to detect the size of the reagent droplets, a reagent droplet size detection sensor (detection optocoupler) is arranged below the reagent cup, the detected size information of the reagent droplets is fed back to a controller of a power source, then the power source controls the action of an extrusion rod, and the extrusion rod is not extruded any more after the reagent droplets with proper size are extruded.
The reagent cup is clamped on the reagent cup rotary table through the reagent cup shell, the clamping bulge is arranged on the reagent cup shell, the clamping groove is arranged on the reagent cup rotary table, after the clamping bulge is embedded into the clamping groove, the reagent cup shell is fixed in the axial direction and the radial direction, and the reagent cup is sleeved on the reagent cup shell.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the utility model discloses a reagent cup, reagent cup carousel and reagent extrusion mechanism, the inside division of reagent cup becomes a plurality of reagent chambeies, be provided with a plurality of reagent outlet pipes on the outside circumference of reagent cup, a reagent chamber disposes a reagent outlet pipe, reagent outlet pipe and reagent chamber intercommunication, the reagent cup carousel is connected on the reagent cup, install the rubber membrane in the junction of reagent carousel and reagent cup, the rubber membrane is used for sealed reagent chamber (guarantee that reagent can only follow the extrusion of reagent outlet pipe, and can not follow the other end outflow of reagent chamber), be provided with a plurality of through-holes and a transmission groove on the reagent carousel, the quantity of through-hole is the same with the reagent chamber, every through-hole communicates with a reagent chamber respectively, the transmission groove cup joints on the output shaft of motor, the motor drives the reagent cup carousel and rotates, reagent extrusion mechanism includes pressure ram and power supply, pressure ram and power supply (motor), Cylinder, oil cylinder, electric push rod, etc.) and the extrusion rod is extruded on the rubber membrane from the through-hole under the effect of power supply, extrudes the reagent from the reagent outlet pipe through the reagent in the rubber membrane deformation extrusion reagent chamber. The extrusion rod is pushed to extrude the rubber membrane, and the reagent in the reagent cavity is extruded out through the deformation of the rubber membrane. Because a plurality of reagent cavities are arranged in the reagent cup, one reagent is stored in each reagent cavity, when the reagent needs to be extruded, the rotating motor rotates, the reagent cavity with the reagent is aligned to the extrusion rod, and then the extrusion rod acts to extrude the reagent. In order to accurately position whether the required reagent chamber is aligned with the extrusion rod, a sensor can be arranged at the extrusion rod to detect whether the reagent chamber is aligned with the extrusion rod. The utility model discloses a reagent package device is for prior art, can provide extruding of multiple reagent, the capillary siphon of being convenient for. The reagent is sucked by hand instead of hand, the efficiency is obviously improved, the reagent is not required to be sucked by hand for many times, the full-automatic operation is realized, and the reagent sucking device can be suitable for a full-automatic chemiluminescence immunoassay device.
2. The utility model discloses still include reagent mount pad, reagent line rail and reagent motor, cup joint the reagent gear on the output shaft of reagent motor, install the reagent rack on the reagent mount pad, reagent gear and reagent rack toothing, the reagent mount pad is installed on the reagent line rail, under the effect of reagent motor, slides on the reagent line rail. The reagent turntable, the rotating motor and the power source are all installed on the reagent installation seat, and thus the reagent cup, the reagent turntable, the rotating motor and the power source slide on the reagent line rail along with the reagent installation seat. The distance between the reagent pack device and the turntable assembly is integrally adjusted, and the capillary tube is ensured to siphon the reagent into the capillary tube.
3. The utility model discloses at reagent droplet size detection sensor of reagent cup below installation (detect the opto-coupler), the controller of power supply is given to the reagent droplet size information feedback that will detect, then the action of power supply control extrusion pole, after extruding the reagent droplet of suitable size, no longer extrudees. Therefore, the amount of capillary siphoning just suitable for one-time extrusion can be achieved, the quantity of reagents is small, reagent waste is avoided, reagent liquid drops do not need to be extruded for many times, and efficiency is improved.
4. The utility model discloses the reagent cup passes through reagent cup shell joint on the reagent cup carousel, and it is protruding to be provided with the joint on the reagent cup shell, is provided with the joint recess on the reagent cup carousel, after embedding the joint recess through the joint arch, and the reagent cup shell is fixed with the equal cup in footpath in the axial, and the reagent cup joints on the reagent cup shell. The reagent cup is arranged on the reagent cup turntable in a clamping manner, so that the reagent cup is convenient to replace and is filled with reagents.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of another view direction structure of the present invention;
FIG. 3 is a schematic view of the reagent cup of the present invention;
FIG. 4 is a schematic view of the structure of the rubber film on the reagent cup cover of the present invention;
FIG. 5 is a schematic view of the structure of the reagent cup housing of the present invention;
FIG. 6 is a schematic view of the reagent cup turntable structure of the present invention;
fig. 7 is a schematic structural diagram of the full-automatic chemiluminescence immunoassay device assembled by matching with other components of the present invention.
Reference numeral 1, a capillary storage assembly, 2, a capillary pushing assembly, 3, a turntable assembly, 4, a blowing assembly, 5, a sample assembly, 6, a reagent pack device, 60, a reagent cup, 61, a reagent cup turntable, 62, a reagent extruding mechanism, 63, a reagent chamber, 64, a reagent outlet pipe, 65, a motor, 66, an extruding rod, 67, a power source, 68, a mounting seat, 69, a reagent track, 610, a reagent motor, 611, a reagent gear, 612, a reagent rack, 613, a reagent cup housing, 614, a droplet size detection sensor, 615, a rubber membrane, 7, a detection assembly, 8, a bottom plate, 9, a capillary.
Detailed Description
The present invention will be further described with reference to the following examples, which are only some, but not all, of the examples of the present invention. Based on the embodiments in the present invention, other embodiments used by those skilled in the art without creative work belong to the protection scope of the present invention. The utility model discloses the position word that appears in like "preceding", "back", "left", "right", has only represented relative position relation, all is the description of going on to the position of figure 7 to the position around not necessarily being actual, consequently can not do the restrictive understanding.
The utility model provides a reagent package device, the effect is for the capillary provides various reagents, for example washing liquid, antibody, reactant, luminous substrate etc.. The reagent pack device has the advantages that a plurality of reagent drops can be provided, and the corresponding reagent drops can be automatically extruded when the reagent drops are used.
The utility model discloses a reagent package device 6 includes reagent cup 60, reagent cup carousel 61 and reagent extrusion mechanism 62, reagent cup 60 internal partitioning becomes a plurality of reagent chambeies 63, be provided with a plurality of reagent outlet pipe 64 on the outside circumference of reagent cup 60, a reagent chamber 63 disposes a reagent outlet pipe 64, reagent outlet pipe and reagent chamber intercommunication, reagent cup carousel 61 is connected on reagent cup 60, install rubber membrane 615 in their junction, seal reagent chamber 63 through rubber membrane, avoid reagent chamber 63 outflow reagent, be provided with a plurality of through-holes and a transmission groove on the reagent carousel 61, the quantity of through-hole is the same with the reagent chamber, every through-hole communicates with a reagent chamber respectively, the transmission groove cup joints on the output shaft of motor 65, motor 65 drives reagent cup carousel 61 and rotates, reagent extrusion mechanism 62 includes pressure ram 66 and power supply 67, pressure ram 66 and power supply 67 (motor, motor 65, reagent pressure pump 67, the pressure pump is connected with the reagent chamber respectively, Cylinder, oil cylinder, electric push rod, etc.) and under the action of the power source, the extrusion rod extends into the extrusion rubber membrane 615 from the through hole, and the rubber membrane deforms and extrudes the reagent in the reagent cavity, so that the reagent is extruded from the reagent outlet pipe. The extrusion rod is pushed to extrude the deformed rubber membrane, and the reagent in the reagent cavity is extruded out by the deformation of the rubber membrane. Because a plurality of reagent cavities are arranged in the reagent cup, one reagent is stored in each reagent cavity, when the reagent needs to be extruded, the rotating motor rotates, the reagent cavity with the reagent is aligned to the extrusion rod, and then the extrusion rod acts to extrude the reagent. In order to accurately position whether the required reagent chamber is aligned with the extrusion rod, a sensor can be arranged at the extrusion rod to detect whether the reagent chamber is aligned with the extrusion rod.
In order to adjust the distance between the reagent pack device and the turntable assembly and siphon the reagent in a capillary tube, the reagent pack device further comprises a reagent mounting seat 68, a reagent line rail 69 and a reagent motor 610, a reagent gear 611 is sleeved on an output shaft of the reagent motor 610, a reagent rack 612 is mounted on the reagent mounting seat 68, the reagent gear 611 is meshed with the reagent rack 612, and the reagent mounting seat 68 is mounted on the reagent line rail 69 and slides on the reagent line rail under the action of the reagent motor. The reagent turntable, the rotating motor and the power source are all installed on the reagent installation seat, and thus the reagent cup, the reagent turntable, the rotating motor and the power source slide on the reagent line rail along with the reagent installation seat. The distance between the reagent pack device and the turntable assembly is integrally adjusted, and the capillary tube is ensured to siphon the reagent into the capillary tube.
In order to detect the size of the reagent droplets, a reagent droplet size detection sensor 614 (detection optocoupler) is arranged below the reagent cup, the detected size information of the reagent droplets is fed back to a controller of a power source, then the power source controls the action of an extrusion rod, and the extrusion rod does not extrude the reagent droplets with proper sizes.
The reagent cups 60 are clamped on the reagent cup rotating disc 61 through the reagent cup shell 613, clamping protrusions are arranged on the reagent cup shell, clamping grooves are arranged on the reagent cup rotating disc, after the clamping protrusions are embedded into the clamping grooves, the reagent cup shell is axially and radially fixed, and the reagent cups are sleeved on the reagent cup shell.
In order that the turntable assembly can accurately and accurately convey the capillary to the reagent position, the reagent position is provided with a position sensor for detecting whether the capillary reaches the position or not, so that extruded reagent liquid drops can be siphoned by the capillary, and the waste of the reagent liquid drops caused by the phenomenon that the capillary does not reach the position due to the extrusion of the reagent liquid drops is avoided.
The utility model provides a full-automatic chemiluminescence immunoassay device, the device's effect is all steps of realizing blood sample chemiluminescence immunoassay, and whole automation mechanized operation, the user only need load the blood sample, and the starting equipment can carry out full automated inspection, the automated generation testing result. The device has the following specific structure:
the method comprises the following steps: capillary storage component 1, capillary push-out component 2, carousel component 3, gas blowing component 4, sample subassembly 5, reagent package device 6, determine module 7 and bottom plate 8, above-mentioned subassembly is all installed on bottom plate 8. Their relative positional relationship is: capillary release subassembly 2 is located the left side of capillary storage subassembly 1, carousel subassembly 3 is located the right side of capillary storage subassembly 1, blowing subassembly 4 is located carousel subassembly 3's left side, and blowing subassembly 4 is located capillary storage subassembly 1 and capillary release subassembly 2's front side, and capillary storage subassembly 1 and capillary release subassembly 2 pass through the mounting panel and install in the left side of 3 side parts on carousel subassembly, and the left side in carousel subassembly 3 the latter half is installed to blowing subassembly 4. The sample assembly 5, the reagent pack device 6 and the detection assembly 7 are all located on the right side of the turntable assembly 3, the reagent pack device 6 is located between the sample assembly 5 and the detection assembly 7, the sample assembly 5 is located on the front right side of the turntable assembly, and the detection assembly is located on the right rear side of the turntable assembly. And a waste liquid groove is arranged below the sample assembly, the air blowing assembly and the reagent package and is arranged below the bottom plate, and the waste liquid groove is used for collecting waste liquid generated by the sample assembly, the air blowing assembly and the reagent package device.
The capillary tube pushing assembly 2 pushes out the capillary tube in the capillary tube storage assembly 1 and sends the capillary tube to the turntable assembly 3, the turntable assembly 3 rotates to convey the capillary tube 9 to the positions of the sample assembly 5, the blowing assembly 4, the reagent pack device 6 and the detection assembly 7, the blowing assembly is used for removing residual liquid in the capillary tube 9, the sample assembly is used for providing a blood sample for the capillary tube, the reagent pack device is used for providing a reagent for the capillary tube, and the detection assembly is used for detecting the number of photons emitted by the capillary tube.
Claims (5)
1. A reagent package device for full-automatic chemiluminescence immunoassay is characterized in that: the reagent cup comprises a reagent cup, a reagent cup turntable and a reagent extrusion mechanism, wherein the inside of the reagent cup is divided into a plurality of reagent cavities, a plurality of reagent outlet pipes are arranged on the outer circumference of the reagent cup, one reagent cavity is provided with one reagent outlet pipe, the reagent outlet pipe is communicated with the reagent cavity, the reagent cup turntable is connected onto the reagent cup, a rubber film is arranged at the joint of the reagent cup and the reagent cup turntable, the reagent cavity is connected with the rubber film, a plurality of through holes and a transmission groove are arranged on the reagent turntable, the number of the through holes is the same as that of the reagent cavities, each through hole is respectively communicated with one reagent cavity, the transmission groove is sleeved on an output shaft of a motor, and the motor drives the reagent cup turntable to rotate.
2. The reagent pack device for full-automatic chemiluminescence immunoassay according to claim 1, wherein: reagent extrusion mechanism includes extrusion stem and power supply, and the extrusion stem links to each other with the power supply, and under the effect of power supply, the extrusion stem stretches into from the through-hole and contacts with the rubber membrane, extrudees the reagent in the reagent chamber through extrusion rubber membrane, extrudees reagent from the reagent outlet pipe.
3. The reagent pack device for full-automatic chemiluminescence immunoassay according to claim 1, wherein: the reagent package device further comprises a reagent mounting seat, a reagent line rail and a reagent motor, a reagent gear is sleeved on an output shaft of the reagent motor, a reagent rack is mounted on the reagent mounting seat and meshed with the reagent rack, the reagent mounting seat is mounted on the reagent line rail and slides on the reagent line rail under the action of the reagent motor, and the reagent turntable, the rotating motor and the power source are mounted on the reagent mounting seat.
4. The reagent pack device for full-automatic chemiluminescence immunoassay according to claim 1, wherein: a reagent droplet size detection sensor is arranged below the reagent cup, the detected reagent droplet size information is fed back to a controller of a power source, then the power source controls the action of an extrusion rod, and after reagent droplets with proper sizes are extruded, the extrusion is not carried out.
5. The reagent pack device for full-automatic chemiluminescence immunoassay according to claim 1, wherein: the reagent cup is clamped on the reagent cup rotary table through the reagent cup shell, the clamping bulge is arranged on the reagent cup shell, the clamping groove is arranged on the reagent cup rotary table, after the clamping bulge is embedded into the clamping groove, the reagent cup shell is fixed in the axial direction and the radial direction, and the reagent cup is sleeved on the reagent cup shell.
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CN201921361514.4U CN210534176U (en) | 2019-08-21 | 2019-08-21 | Reagent package device for full-automatic chemiluminescence immunoassay |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110514858A (en) * | 2019-08-21 | 2019-11-29 | 成都斯马特科技有限公司 | A kind of automatic chemiluminescence immunoassay reagent bag apparatus |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110514858A (en) * | 2019-08-21 | 2019-11-29 | 成都斯马特科技有限公司 | A kind of automatic chemiluminescence immunoassay reagent bag apparatus |
CN110514858B (en) * | 2019-08-21 | 2024-06-18 | 成都斯马特科技股份有限公司 | Reagent package device for full-automatic chemiluminescence immunoassay |
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Address after: No.1, 1st floor, building 1, No.333 Hezuo Road, high tech Zone, Chengdu, Sichuan 610000 Patentee after: Chengdu Smart Technology Co.,Ltd. Country or region after: China Address before: No.1, 1st floor, building 1, No.333 Hezuo Road, high tech Zone, Chengdu, Sichuan 610000 Patentee before: CHENGDU SEAMATY TECHNOLOGY Co.,Ltd. Country or region before: China |