CN209768299U - Double-layer potting device for obtaining root tips of a large number of plants - Google Patents

Double-layer potting device for obtaining root tips of a large number of plants Download PDF

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Publication number
CN209768299U
CN209768299U CN201822257334.3U CN201822257334U CN209768299U CN 209768299 U CN209768299 U CN 209768299U CN 201822257334 U CN201822257334 U CN 201822257334U CN 209768299 U CN209768299 U CN 209768299U
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China
Prior art keywords
flowerpot
wall
pot
double
root
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Expired - Fee Related
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CN201822257334.3U
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Chinese (zh)
Inventor
唐祈林
李晓锋
张萍
周阳
何如钰
李杨
李影正
吴子洲
温晓东
张磊
严旭
何建美
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Sichuan Agricultural University
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Sichuan Agricultural University
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Priority to CN201822257334.3U priority Critical patent/CN209768299U/en
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Abstract

The utility model discloses a acquire double-deck device cultivated in a pot of a large amount of plant root tips many times. The utility model is characterized in that one flowerpot is sleeved in the other flowerpot, the water-retaining agent is filled between the walls of the two flowerpots at intervals, and the wall of the flowerpot inside is provided with small holes, so that roots can be exposed conveniently; the wall of the outer flowerpot can be opened and closed. The utility model can be used for repeatedly obtaining the root tips of plants without damaging the plants; secondly, the obtained plant root tips are large in quantity and good in quality; the root system is induced to penetrate through the small holes in the inner layer of the flowerpot by utilizing the water and the fertilizer, the flowerpot wall outside the flowerpot is opened, the root tip can be directly cut, the taken root tip is clean, washing is not needed, and the method is simple, convenient and labor-saving; the water-retaining agent is nontoxic, pollution-free and reusable.

Description

Double-layer potting device for obtaining root tips of a large number of plants
Technical Field
The utility model belongs to the field of plant cytogenetics, in particular to a double-layer pot plant device for acquiring a large number of plant root tips for a plurality of times.
Background
In plant cytology research and distant hybridization breeding, the chromosome number and chromosome composition of plant cells are often identified, and the root tip is a common material for identifying the chromosome number and chromosome composition of plant cells. At present, there are two main methods for obtaining plant root tips: (1) the method for germinating and rooting seeds is simple and feasible, but requires a large amount of materials and a sufficient number of seeds. (2) A method for taking roots from plants mainly aims at materials with small seed quantity or vegetative propagation materials, and three methods are provided for taking roots from plants: (a) the water culture root-taking method needs to control the temperature, the moisture, the nutrients and the like, if the control is improper, the root rot is easy to cause, and in addition, the chromosome splitting phase state of a water culture plant is not as good as the phase state of soil culture; (b) the method for cutting soil and taking roots in field planting has the advantages that the root taking direction of field plants is not clear due to the fact that soil is not transparent, the complete plant root system cannot be obtained, conditions such as temperature, illumination, moisture and nutrients in fields are not easy to control, the growth state of roots is difficult to control, and the method for taking roots is time-consuming, labor-consuming and poor in effect; (c) the method is easy to control the conditions of temperature, illumination, moisture, nutrients and the like, but the root of the plant needs to be taken out of the pot when the root is taken by the method, most of the root of the plant is interweaved together in an intricate and complex manner and is adhered to the soil together in the growth process of the plant, and the plant is extremely difficult to take out because the soil is partially adhered in the flowerpot; secondly, because the plants are taken out of the pot without medium, if the roots of the plants are too long, the plants can be wound on the bottom of the pot, the plants are taken out forcibly, the attached soil is easy to collapse, and the whole root system can be damaged inevitably, so that the growth of the plants is adversely affected; in addition, the soil in the flowerpot is relatively loose, a certain soil form needs to be maintained by the flowerpot, if a plant is forcibly taken, the soil is loose, the root structure of the plant can be damaged, and the growth and development of the plant are seriously influenced. The method for obtaining the plant root tip has the problems of time and labor waste, poor root taking effect and the like, and influences the normal growth of plants after the root is taken, thus damaging the growth and development of the plants.
In order to overcome the problems of the root taking method, a patent of a pot plant device for obtaining a large number of plant root tips for multiple times (CN206575898U) discloses a pot plant device, which is mainly characterized in that a plastic film with holes is flatly paved at the bottom of a flowerpot, the two ends of the plastic film symmetrically extend out of a flowerpot opening along the inner wall of the flowerpot, the plastic film is lifted when the roots are taken, the whole plant is taken out, and the root tips are cut around the attached soil. Although the method is improved compared with the traditional potted plant root taking method, the root taking efficiency is improved, and the plant is protected to a certain extent, if the plant is larger like a corn root system, the plant can be taken out from the flowerpot by spending more strength, and if the cultivation time is long, the soil is caked and is not easy to take out when being clamped in the flowerpot; in addition, if the culture medium is a nutrient soil or other medium, the texture is loose, which inevitably causes the falling of the medium attached to the root system, damages the fibrous root system of the plant and is not beneficial to the continuous culture of the plant.
In summary, the existing methods for obtaining plant root tips are time-consuming and labor-consuming, have poor root-taking effect, and have great damage to plants after root taking, which affects normal growth of plants, so it is necessary to find a root-taking device that can obtain a large amount of high-quality root tips for many times, and at the same time, will not damage the root systems required by normal growth of plants, so that the plants can continue to grow normally, so as to facilitate later observation and subsequent research work.
SUMMERY OF THE UTILITY MODEL
To there being time-consuming, hard, the poor scheduling problem of effect in obtaining the root tip method from the plant at present, the utility model aims to provide a device cultivated in a pot of double-deck of obtaining the plant root tip many times. The root tip taking device has the advantages of simplicity, rapidness, convenience, low cost and the like, and can not influence the normal growth and development of plants, and the plants can be continuously cultured after the root taking is finished so as to facilitate the subsequent experiments.
in order to achieve the above purpose, the technical scheme of the utility model is as follows:
A double-layer pot culture device for obtaining a large number of plant root tips for multiple times comprises a flowerpot I1 and a flowerpot II2, wherein a flowerpot I1 is nested in the flowerpot II2, the space between the wall of the flowerpot I1 and the wall of the flowerpot II2 is 3-5 cm, and a small hole is formed in the wall of the flowerpot I1.
In the double-layer pot culture device, the flowerpot II2 comprises a base 25, and 2 symmetrical walls I21 and II22 are arranged on the base 25; wall I21 and wall II22 are connected by hinge 23, hinge 23 is fixed to wall I21 and wall II22 by screws 24 respectively; the other side of the wall I21 is provided with a clamping groove, and the corresponding part of the wall II22 is provided with a clamping pin 26. Wherein wall I21 and wall II22 are each symmetrical halves of the wall of pot II. Wall I21 and wall II22 can be opened and closed in parallel by hinge 23, and when closed, clip 26 is clipped into the clip groove of wall I21 to fix wall I21 and wall II 22.
In the double-layer pot culture device, the diameter of the small hole in the wall of the flowerpot I1 is 2-4 mm; the spacing distance between the small holes is 4-5 mm.
In the double-layer pot culture device, the middle of the bottom of the flowerpot I1 is provided with a hole, and the diameter of the hole is 1-2 cm; the number of the holes is 2-5. The use of the pores is for water penetration.
In the double-layer pot culture device, the middle of the base 25 of the flowerpot II2 is provided with a hole, and the diameter of the hole is 1-2 cm; the number of the holes is 2-5. The use of the pores is for water penetration.
In the double-layer pot culture device, the flowerpot I1 and the flowerpot II2 are fixed through a concave-convex structure at the bottom; the bottom of the flowerpot I1 is provided with a depression, the base 25 of the flowerpot II2 is provided with a bulge, and the depression at the bottom of the flowerpot I1 corresponds to the bulge on the base 25 of the flowerpot II 2.
In the double-layer pot culture device, the diameter of the pot opening of the flowerpot I1 is smaller than that of the pot opening of the flowerpot II 2.
In the double-layer pot culture device, the diameter of the bottom of the flowerpot I1 is smaller than that of the base 25 of the flowerpot II 2.
Preferably, the height of the wall of the flowerpot I1 is greater than that of the wall of the flowerpot II2, namely, the pot mouth of the flowerpot I1 is higher than that of the flowerpot II 2.
The flowerpot I1 is in the shape of an inverted round table or a cylinder.
The flowerpot II2 is in the shape of an inverted round table or a cylinder.
The shape of the flowerpot I1 is matched with that of the pot II 2.
The flowerpot II2 is made of hard plastic with high strength, such as high-density polyethylene, so that the flowerpot II2 can be repeatedly used for multiple times.
The flowerpot I1 is made of common flowerpot materials or reticular inverted round table materials with certain supporting capacity, such as a stainless steel net.
And a water-retaining agent is filled between the wall of the flowerpot I1 and the wall of the flowerpot II 2. The water retention capacity of the water retention agent is large.
The water-retaining agent is a macromolecule water-retaining agent (SAP).
Compared with the prior art, the utility model has the advantages and beneficial effect: (1) the utility model discloses can be used to repeated a large amount of plant root tips of acquireing, provide a simple and easy swift, and do not harm the good instrument of the acquisition root tip of plant completely for plant cell genetics research. (2) And application the utility model discloses the plant root tip that acquires is in large quantity, of high quality. (3) The utility model discloses utilize the root to liquid manure nature, it is convenient not only to get the root, does benefit to the plant growth moreover. (4) The utility model discloses utilize liquid manure induced root system to pass flowerpot inlayer aperture, open outer flowerpot wall, can directly cut and get root tip, simple and convenient. (5) And the application of the utility model discloses gained plant root tip is clean, need not wash. (6) And use the utility model discloses need not to take out the plant from the flowerpot when getting the root tip, easy operation, laborsaving. (7) The utility model discloses pack the water-retaining agent at the intermediate layer, itself has the water effect of protecting against drought, forms miniature reservoir around the root, can protect water and protect fertile, improves quality cultivated in a pot. (8) The water-retaining agent used in the utility model is nontoxic, pollution-free and reusable. (9) The utility model discloses it is with low costs to acquire the root tip.
Drawings
FIG. 1 is a front view of the present invention; wherein 1 is a flowerpot I; and 2 is a flowerpot II.
Fig. 2 is a sectional view of the present invention; wherein 1 is a flowerpot I; and 2 is a flowerpot II.
Fig. 3 is a schematic structural diagram of a flowerpot I.
FIG. 4 is a schematic structural view of a flowerpot II; wherein 21 is wall I; 22 is wall II; 23 is a hinge; 24 is a screw; and 25 is a base.
FIG. 5 is a schematic structural view of a wall of a flowerpot II; wherein 21 is wall I; 22 is wall II; 23 is a hinge; 24 is a screw; 25 is a base; 26 is a clip.
Fig. 6 is a bottom structure schematic diagram of the flowerpot I.
fig. 7 is a structural schematic view of the base 25 of the flowerpot II.
Detailed Description
Embodiment 1 manufacture of the double-layered potting device of the present invention
The following describes the fabrication of the double-layered potting device of the present invention in detail with reference to FIGS. 1 to 7
1. A common plastic flowerpot is purchased from the market as a flowerpot I1, the diameter of the mouth of the flowerpot I1 is 30cm, the diameter of the bottom of the flowerpot I1 is 19cm, and the height of the wall of the flowerpot I1 is 24 cm; the bottom of the groove is provided with 3 grooves (see figure 6); holes are punched in the wall of the flowerpot I1 according to the figure 3, the diameter of the punched holes is 3mm, and the distance between the holes is 4 mm.
2. And (3) manufacturing a flowerpot II: the flowerpot II2 is manufactured according to the figures 4, 5 and 7. The flowerpot II2 is made of hard plastic. The pot opening of pot II2 was 33cm in diameter, the base 22cm in diameter, the height of the wall of pot II2 was 20cm, and the height of the wall of pot II2 was less than the height of the wall of pot I1, as shown in fig. 1 and 2. The wall of the flowerpot II consists of a wall I21 and a wall II22 which are symmetrical, the wall I21 and the wall II22 are connected together through a hinge 23, and the hinge 23 is fixed on the wall I21 and the wall II22 through screws 24. The base 25 of pot II2 has 3 protrusions (see fig. 7) for holding pot I1. On one side of wall I21 there is a catch and on the corresponding part of wall II22 there is a movable catch 26.
3. Placing the sunken part at the bottom of the flowerpot I1 at the raised position of the base of the flowerpot II2 for fixing; the wall I21 and the wall II22 of the flowerpot II are closed, and the clip 26 is clamped in the clamping groove of the wall I21, so that the utility model is completed (see fig. 1 and fig. 2).
Embodiment 2 the utility model discloses use of double-deck device cultivated in a pot
1. The double-layered potting device of the present invention manufactured in example 1 was filled with a substrate. Namely, nutrient soil is filled in the flowerpot I1, and the nutrient soil is prepared by mixing soil and nutrients according to the proportion of 2: 1. The space between the wall of the flowerpot I1 and the wall of the flowerpot II2 is filled with a macromolecule water-retaining agent.
2. seed soaking and germination: placing corn seeds in water, and soaking for 5h at 25 ℃; and (3) sowing the soaked corn seeds in nutrient soil of a flowerpot I1, wherein the sowing depth is 2-3 cm, and normally watering until the corn seeds germinate.
3. Stopping watering in a flowerpot I1 after the seeds germinate and grow seedlings; and watering the water-retaining agent between the flowerpot I1 and the flowerpot II2 to ensure that the water-retaining agent absorbs water to be saturated.
4. Taking root tips: after culturing for 2-3 weeks, opening the wall I21 and the wall II22 of the flowerpot II2, observing whether the root tip is exposed out of the small hole of the flowerpot I1, if the root tip is exposed out of the small hole, shearing the root tip with the length of about 1cm by using small scissors, and storing for later use.
5. After the root taking is finished, closing the wall I21 and the wall II22 of the flowerpot II2, clamping the clip 26 on the clamping groove of the wall II22, filling a water-retaining agent between the wall of the flowerpot I1 and the wall of the flowerpot 2, and continuously culturing the plants so as to take the roots or observe the roots in the following process.

Claims (6)

1. A double-layer pot culture device for obtaining a large number of plant root tips repeatedly is characterized by comprising a flowerpot I (1) and a flowerpot II (2), wherein the flowerpot I (1) is nested in the flowerpot II (2), the interval between the wall of the flowerpot I (1) and the wall of the flowerpot II (2) is 3-5 cm, and small holes are formed in the wall of the flowerpot I (1);
The flowerpot II (2) comprises a base (25), and 2 symmetrical walls I (21) and II (22) are arranged on the base (25); the wall I (21) and the wall II (22) are connected together through a hinge (23), and the hinge (23) is fixed on the wall I (21) and the wall II (22) through screws (24); the other side of the wall I (21) is provided with a clamping groove, and the corresponding part of the wall II (22) is provided with a clamping needle (26);
The flowerpot I (1) and the flowerpot II (2) are fixed through a concave-convex structure at the bottom; the bottom of the flowerpot I (1) is provided with a depression, the base (25) of the flowerpot II (2) is provided with a bulge, and the depression at the bottom of the flowerpot I (1) corresponds to the bulge on the base (25) of the flowerpot II (2);
The diameter of the pot opening of the flowerpot I (1) is smaller than that of the pot opening of the flowerpot II (2); the diameter of the bottom of the flowerpot I (1) is smaller than that of the base (25) of the flowerpot II (2);
The height of the wall of the flowerpot I (1) is greater than that of the wall of the flowerpot II (2), namely the pot mouth of the flowerpot I (1) is higher than that of the flowerpot II (2).
2. A double pot apparatus as claimed in claim 1, wherein the diameter of the holes in the wall of the pot I (1) is 2-4 mm; the spacing distance between the small holes is 4-5 mm.
3. The double-layered pot culture device according to claim 1 or 2, wherein holes are formed in the middle of the bottom of the flowerpot I (1), the diameter of each hole is 1-2 cm, and the number of the holes is 2-5; the middle of the base (25) of the flowerpot II (2) is provided with a hole, the diameter of the hole is 1-2 cm, and the number of the holes is 2-5.
4. A double pot apparatus as claimed in claim 3, wherein said pot I (1) is in the shape of an inverted circular truncated cone or a cylinder; the flowerpot II (2) is in an inverted round table or cylindrical shape; the shape of the flowerpot I (1) is matched with that of the flowerpot II (2).
5. The double-layered pot culture device according to claim 4, wherein the flowerpot II (2) is made of hard plastic with high strength; the flowerpot I (1) is made of a common plastic flowerpot material or a stainless steel mesh material with certain supporting capacity.
6. The double-layered pot culture device according to claim 5, wherein a water-retaining agent is filled between the wall of the flowerpot I (1) and the wall of the flowerpot II (2); the water-retaining agent is a macromolecule water-retaining agent.
CN201822257334.3U 2018-12-29 2018-12-29 Double-layer potting device for obtaining root tips of a large number of plants Expired - Fee Related CN209768299U (en)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112535080A (en) * 2020-12-10 2021-03-23 浙大宁波理工学院 Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines
CN113303204A (en) * 2021-06-23 2021-08-27 浙江农林大学暨阳学院 Culture medium for potted peony, culture pot and culture method
CN114041385A (en) * 2021-11-15 2022-02-15 宁波市农业科学研究院 Research method for root growth and development of vegetable and fruit crops
CN114158388A (en) * 2021-11-26 2022-03-11 安徽省农业科学院农业工程研究所 Cultivation device for molding rose roots

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112535080A (en) * 2020-12-10 2021-03-23 浙大宁波理工学院 Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines
CN112535080B (en) * 2020-12-10 2022-04-08 浙大宁波理工学院 Method for improving formation rate of fusiform tuberous roots for combined spicebush root medicines
CN113303204A (en) * 2021-06-23 2021-08-27 浙江农林大学暨阳学院 Culture medium for potted peony, culture pot and culture method
CN114041385A (en) * 2021-11-15 2022-02-15 宁波市农业科学研究院 Research method for root growth and development of vegetable and fruit crops
CN114158388A (en) * 2021-11-26 2022-03-11 安徽省农业科学院农业工程研究所 Cultivation device for molding rose roots

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Granted publication date: 20191213

Termination date: 20201229