CN206127326U

CN206127326U - A fixed bed device for cell culture

Info

Publication number: CN206127326U
Application number: CN201620801345.1U
Authority: CN
Inventors: 白仲虎; 聂简琪; 孙杨; 郭雯雯
Original assignee: CHANGZHOU YINGDE BIOTECHNOLOGY Co Ltd; Jiangnan University
Current assignee: CHANGZHOU YINGDE BIOTECHNOLOGY Co Ltd; Jiangnan University
Priority date: 2016-07-28
Filing date: 2016-07-28
Publication date: 2017-04-26
Anticipated expiration: 2026-07-28

Abstract

The utility model provides a fixed bed device for cell culture, it can solve culture medium undercompounding, and cell growth is slow, cell density low grade technical problem. It includes mixing arrangement and carrier fixing device, its characterized in that: mixing arrangement includes impeller seat, and the lower extreme opening of impeller seat's cavity closes through the lower cover lid, is equipped with down the pivot on the apron, and install impeller is in the pivot, and impeller seat lies in on the position of impeller downside or is equipped with the feed liquor hole down on the apron, carrier fixing device includes the container, and impeller seat installs and communicates in the container lower extreme and with the container.

Description

A kind of fixed bed device for cell culture

Technical field

This utility model is related to animal cell culture art field, more particularly to a kind of fixed bed for cell culture is filled Put.

Background technology

Fixed bed reactors can reach higher cell concentration in relatively small volume, and cell density is usual 10 can be reached⁸Individual/mL, about other modes（Suspension culture）10 times.Existing fixed bed reactors include and carrier Fixing device and two parts of fluid reservoir, fixed bed top is pumped into by peristaltic pump by culture medium from fluid reservoir, and culture medium is worn Cross fixed bed and return in fluid reservoir from fixed bed bottom, circulate successively.Although this training method can avoid culture medium Using waste, but cannot be sufficiently mixed between original culture medium in new culture medium and fixed bed in fixed bed, cause Cell growth is slow, and cell density is low, affects cell culture quality.

Utility model content

For the problems referred to above, this utility model provides a kind of fixed bed device for cell culture, and it can solve training Foster base undercompounding, cell growth is slow, the low technical problem of cell density.

A kind of fixed bed device for cell culture, it includes mixing arrangement and carrier fixing device, it is characterised in that：

The mixing arrangement includes impeller seat, and the lower ending opening of the cavity of the impeller seat is covered by lower cover, described Lower cover is provided with rotating shaft, and impeller is installed in rotating shaft, and the impeller seat is located on the position on the downside of the impeller or described Lower cover is provided with inlet opening；

The carrier fixing device includes container, and the impeller seat is installed on the container lower end and connects with the container It is logical.

Further, the mixing arrangement also includes magnetically-actuated motor, and the impeller is magnetic impeller or is provided with magnetic Body, to be driven by the magnetically-actuated motor.

Further, the mixing arrangement also includes motor, and the rotating shaft is connected or for motor with the output shaft of motor Output shaft.

Further, it is sequentially installed with from bottom to top in the cavity of the container under carrier and is supported on support meanss and carrier Device, the region that the cavity of the container is located under the carrier on support meanss, the carrier between support meanss is carrier Rest area, the upper and lower both sides of the carrier rest area connect respectively with the cavity,

Further, the upper support meanss are movably installed in the cavity of the container, and can be along the cavity Move up and down.

Further, the upper support meanss are overhead gage, and the overhead gage is circumferentially with outer lug or external groove, institute The cavity inner wall for stating container is provided with the inner groovy or internal projection coordinated with the outer lug or external groove.

Further, the impeller seat is columnar structured, and the lower cover is integrally formed with the impeller seat.

This utility model additionally provides a kind of cell reactor, it is characterised in that：It includes above-mentioned fixed bed device, pedestal And reaction vessel, the impeller seat is fixed on the pedestal, and the impeller seat lower surface and the pedestal upper surface it Between be provided with gap so that the inlet opening and the impeller seat external communication, the pedestal is fixed in the reaction vessel.

Further, the impeller seat lower end is provided with some spaced pins, the pedestal and the pin pair The position answered is provided with some slots, and the pin is plugged in the slot.

Fixed bed device of the present utility model, carrier is positioned in carrier rest area, and by support meanss under carrier and Support meanss are fixed on carrier, impeller rotation, promote the culture medium in the cavity of impeller seat to flow up, and flow through carrier rest area Flow out from the open top of container afterwards, while negative pressure is formed in the cavity of impeller seat, by the culture medium outside cavity from feed liquor Hole sucks so that culture medium promotes mixing and the gas transfer of culture medium in cell reactor internal circulation flow, improves cell The speed of growth, increases cell density, it is ensured that cell culture quality；And the fixed bed device integration degree is high, simple structure Rationally；Additionally, the cell reactor containing the fixed bed device can be sufficiently mixed and utilize cell culture medium, obtain faster Expected cell quantity, improves research and development and production efficiency.

Description of the drawings

Fig. 1 is the decomposition texture schematic diagram of fixed bed device of the present utility model and pedestal connection；

Fig. 2 is the structural representation of impeller seat of the present utility model；

Fig. 3 is the structural representation of cell reactor of the present utility model.

Specific embodiment

As shown in Figure 1 to Figure 3, a kind of fixed bed device for cell culture, it includes that mixing arrangement and carrier are fixed Device,

Mixing arrangement includes columnar structured impeller seat 1, and the lower ending opening of the cavity 12 of impeller seat 1 is by being integrally formed Lower cover 13 cover, lower cover 13 is provided with rotating shaft 14, and impeller 3 is installed in rotating shaft 14, and lower cover upper measurement 14 sets around the shaft There is inlet opening 15, positioned at impeller seat lower section magnetically-actuated motor 7 is provided with, impeller is magnetic impeller or is provided with magnet, to pass through Magnetically-actuated horse 7 is up to driving；

Carrier fixing device includes container 4, and impeller seat 1 is installed on the lower end of container 4 and connects with container 4, the chamber of container 4 Support meanss 52 support meanss 51 and carrier in are sequentially installed with carrier from bottom to top in vivo, and the cavity of container is located under carrier Region on support meanss, carrier between support meanss is carrier rest area, the upper and lower both sides of carrier rest area respectively with cavity Connection；

Upper support meanss 52, lower support meanss 51 are respectively and are evenly distributed with overhead gage, the lower baffle plate of perforation, overhead gage, Lower baffle plate is circumferentially with outer lug 53, and the cavity inner wall of container is provided with the inner groovy 41,42 with outer lug, and inner groovy 41 is L Shape structure, is easy to the sealed fixation of overhead gage；Additionally, upper support meanss 52 are also movable be installed in the cavity of container, and energy It is enough to move up and down along cavity, the carrier of different-thickness is conveniently adapted to, it is overhead gage for example to go up support meanss, and overhead gage periphery sets The cavity inner wall for having external screw thread, container is provided with axially distributed female thread, and overhead gage is threadedly coupled with the cavity of container.

This utility model additionally provides a kind of cell reactor, and it includes above-mentioned fixed bed device, pedestal 6 and reaction vessel 8, impeller seat 1 is fixed on pedestal 6, and is provided with gap between impeller seat lower surface and pedestal upper surface so that inlet opening with Impeller seat external communication, pedestal is fixed in reaction vessel；Specifically, impeller seat lower end is provided with some spaced pins 11, gap 16 is formed between adjacent pin 11, pedestal upper surface is convexly equipped with and is provided with some slots 61 with the corresponding position of pin 11, Pin 11 is plugged in slot 61；Wherein, in Fig. 39 be culture medium pipette, 10 be culture medium feed tube, fixed bed device work After work, the culture medium in reaction vessel 8 circulates mode as shown by arrows.

Claims

1. a kind of fixed bed device for cell culture, it includes mixing arrangement and carrier fixing device, it is characterised in that：

The mixing arrangement includes impeller seat, and the lower ending opening of the cavity of the impeller seat is covered by lower cover, the lower cover Plate is provided with rotating shaft, and impeller is installed in rotating shaft, and the impeller seat is located on the position on the downside of the impeller or the lower cover Plate is provided with inlet opening；

The carrier fixing device include container, the impeller seat be installed on the container lower end and with the reservoir.

2. a kind of fixed bed device for cell culture according to claim 1, it is characterised in that：The mixing arrangement Also include magnetically-actuated motor, the impeller is magnetic impeller or is provided with magnet, to drive by the magnetically-actuated motor.

3. a kind of fixed bed device for cell culture according to claim 1, it is characterised in that：The mixing arrangement Also include motor, the rotating shaft is connected or for the output shaft of motor with the output shaft of motor.

4. a kind of fixed bed device for cell culture according to claim 1 or 2 or 3, it is characterised in that：The appearance Support meanss support meanss and carrier in are sequentially installed with carrier in the cavity of device from bottom to top, and the cavity of the container is located at Region under the carrier on support meanss, the carrier between support meanss is carrier rest area, the carrier rest area Upper and lower both sides connect respectively with the cavity.

5. a kind of fixed bed device for cell culture according to claim 4, it is characterised in that：The upper support fills Put and be movably installed in the cavity of the container, and can move up and down along the cavity.

6. a kind of fixed bed device for cell culture according to claim 5, it is characterised in that：The upper support fills It is set to overhead gage, the overhead gage is circumferentially with outer lug or external groove, the cavity inner wall of the container is provided with outer with described Inner groovy or internal projection that raised or external groove coordinates.

7. a kind of fixed bed device for cell culture according to claim 1, it is characterised in that：The impeller seat is Columnar structured, the lower cover is integrally formed with the impeller seat.

8. a kind of cell reactor, it is characterised in that：It includes such as arbitrary fixed bed device in claim 1 ~ 3, and Pedestal and reaction vessel, the impeller seat is fixed on the pedestal, and the impeller seat lower surface and the pedestal upper end Gap is provided between face, so that the inlet opening and the impeller seat external communication, the pedestal is fixed on the reaction vessel It is interior.

9. a kind of cell reactor according to claim 8, it is characterised in that：The impeller seat lower end is provided with some intervals The pin of setting, pedestal position corresponding with the pin is provided with some slots, and the pin is plugged in the slot.