CN206127331U - Mixing arrangement for cell reactor - Google Patents
Mixing arrangement for cell reactor Download PDFInfo
- Publication number
- CN206127331U CN206127331U CN201620801386.0U CN201620801386U CN206127331U CN 206127331 U CN206127331 U CN 206127331U CN 201620801386 U CN201620801386 U CN 201620801386U CN 206127331 U CN206127331 U CN 206127331U
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- CN
- China
- Prior art keywords
- impeller
- mixing arrangement
- cavity
- cell reactor
- arrangement according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a mixing arrangement for cell reactor, it can solve among the mixing process technical problem that the pair cell caused mechanical damage. It includes impeller seat, and impeller seat is equipped with the cavity, and the lower extreme opening of cavity closes through the lower cover lid, is equipped with down the pivot on the apron, and install impeller is in the pivot and in placing the cavity in, and impeller seat lies in on the position of impeller downside or is equipped with the feed liquor hole on the apron down, and the size in feed liquor hole is lighter than the size of carrier.
Description
Technical field
This utility model is related to animal cell culture art field, more particularly to a kind of cell reactor mixing arrangement.
Background technology
At present still by the way of Mechanical Driven blade stirring, this is mixed the hybrid mode of the commercialization cell reactor of main flow
Conjunction mode studies maturation, easy to operate.It is different from microorganism, due to the acellular wall of zooblast, to shearing extreme sensitivity, no matter
The features such as cell reactor of which kind of form will possess low sheraing effect, good mixing property.Although for cell culture exploitation
Go out low-shearing force blade, but during carrier suspension culture, the carrier for being fitted with cell can be with blade directly contact, can be to thin
Born of the same parents cause mechanical damage.
Utility model content
For the problems referred to above, this utility model provides a kind of cell reactor mixing arrangement, and it can solve to mix
The technical problem of mechanical damage and mixed effect difference is caused in journey to cell.
Its technical scheme is such, and it includes impeller seat, and the impeller seat is provided with cavity, the lower ending opening of the cavity
Covered by lower cover, the lower cover is provided with rotating shaft, impeller is installed in rotating shaft, is placed in the cavity, the leaf
Wheel seat is located on the position on the downside of the impeller or the lower cover is provided with inlet opening, and the inlet opening is smaller in size than load
The size of body.
Further, the mixing arrangement also includes magnetically-actuated motor, and the impeller is magnetic impeller or is provided with magnetic
Body, to be driven by the magnetically-actuated motor.
Further, the mixing arrangement also includes motor, and the rotating shaft is connected or for motor with the output shaft of motor
Output shaft.
Further, the upper end open of the cavity is covered by upper cover plate, and the upper cover plate is provided with liquid outlet.
Further, the liquid outlet is provided with more than 2；Preferably, the inlet opening is located at the diapire of the cavity
Middle part, the liquid outlet is located at the surface that the impeller and cavity enclose the gap to be formed.
Further, the impeller seat is for columnar structured, the upper cover plate or the lower cover and the impeller seat one
It is body formed.
Further, the impeller seat bottom is provided with the fixed support of more than 2, between being provided between adjacent fixed support
Gap.
Further, the fixed support is sucker, alignment pin or locating piece.
Mixing arrangement of the present utility model, when using, is placed in cell reactor（Or fluid reservoir, disposable liquid storing bag etc.
Cell culture associated vessel）Bottom or side wall, impeller rotation, promote the culture medium in cavity from upper end open or liquid outlet stream
Go out, while the formation negative pressure in cavity is so that the culture medium outside cavity to be sucked from the inlet opening of bottom so that culture medium is in cell
Reactor internal circulation flow, promotes mixing and the gas transfer of culture medium, good mixing effect；The mixing arrangement operationally,
Because the size for being smaller in size than carrier of inlet opening, only culture medium can be entered in cavity by inlet opening, impeller pair can be avoided
Cell causes mechanical damage.
Description of the drawings
Fig. 1 is the decomposition texture schematic diagram of an embodiment of the present utility model, wherein eliminating magnetically-actuated motor；
Fig. 2 is the structural representation at another visual angle of impeller seat in Fig. 1；
Fig. 3 is installed on the view after cell reactor for this utility model.
Specific embodiment
As shown in Fig. 1 ~ Fig. 3, it includes columnar structured impeller seat 1, and impeller seat 1 is provided with cavity 12, under cavity 12
End opening is covered by integrally formed lower cover 13, and lower cover 13 is provided with rotating shaft 14, impeller 3 be installed in rotating shaft 14 and
It is placed in cavity 12, the lower section of impeller seat 1 is provided with magnetically-actuated motor 2, impeller is magnetic impeller or is provided with magnet, with
Driven by magnetically-actuated motor；Lower cover upper measurement 14 is provided with inlet opening 15, the chi for being smaller in size than carrier of inlet opening around the shaft
It is very little；
The upper end open of cavity is covered by upper cover plate 4, and upper cover plate is tightly connected with cavity upper end open, can be connected by glue
Or the mode such as sealing ring is set realizes, upper cover plate is provided with 4 upper liquid outlets 41, and liquid outlet 41 is located at impeller and cavity is enclosed
The surface in the gap of formation；
Impeller seat bottom is provided with the locating piece 11 of more than 2, and gap 16 is provided between adjacent positioned block.
5 is the tank body of cell reactor in Fig. 3, and 51 is drain pipe, and 52 is feed tube.
As shown in figure 3, assembling when, impeller seat by locating piece be plugged in cell reactor bottom and lower cover with
Gap is left between the diapire of cell reactor so that culture medium can be from the cavity that inlet opening enters impeller seat；When using,
Magnetically-actuated motor drives impeller rotation, promotes the culture medium in cavity to flow out from upper end open or liquid outlet, while in cavity
Formation negative pressure so that the culture medium outside cavity to be sucked from the inlet opening of bottom so that culture medium is in cell reactor inner recirculation flow
It is dynamic, circulate in directional diagram shown in arrow, to promote mixing and the gas transfer of culture medium.
Claims (9)
1. a kind of cell reactor mixing arrangement, it includes impeller seat, and the impeller seat is provided with cavity, the lower end of the cavity
Opening is covered by lower cover, and the lower cover is provided with rotating shaft, and impeller is installed in rotating shaft, is placed in the cavity, institute
Impeller seat is stated on the position on the downside of the impeller or the lower cover is provided with inlet opening, the size of the inlet opening is little
In the size of carrier.
2. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The mixing arrangement is also wrapped
Magnetically-actuated motor is included, the impeller is magnetic impeller or is provided with magnet, to drive by the magnetically-actuated motor.
3. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The mixing arrangement is also wrapped
Include motor, the rotating shaft is connected or for the output shaft of motor with the output shaft of motor.
4. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：Open the upper end of the cavity
Mouth is covered by upper cover plate, and the upper cover plate is provided with liquid outlet.
5. a kind of cell reactor mixing arrangement according to claim 4, it is characterised in that：The liquid outlet is provided with 2
More than individual.
6. a kind of cell reactor mixing arrangement according to claim 4, it is characterised in that：The inlet opening is located at institute
The middle part of the diapire of cavity is stated, the liquid outlet is located at the impeller and cavity encloses the surface in the gap to be formed.
7. a kind of cell reactor mixing arrangement according to claim 4, it is characterised in that：The impeller seat is cylinder
Shape structure, the upper cover plate or the lower cover are integrally formed with the impeller seat.
8. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The impeller seat bottom sets
There is the fixed support of more than 2, between adjacent fixed support gap is provided with.
9. a kind of cell reactor mixing arrangement according to claim 8, it is characterised in that：The fixed support is to inhale
Disk, alignment pin or locating piece.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620801386.0U CN206127331U (en) | 2016-07-28 | 2016-07-28 | Mixing arrangement for cell reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620801386.0U CN206127331U (en) | 2016-07-28 | 2016-07-28 | Mixing arrangement for cell reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206127331U true CN206127331U (en) | 2017-04-26 |
Family
ID=58563385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620801386.0U Expired - Fee Related CN206127331U (en) | 2016-07-28 | 2016-07-28 | Mixing arrangement for cell reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206127331U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047705A (en) * | 2016-07-28 | 2016-10-26 | 江南大学 | Mixing device of cell reactor and application thereof |
-
2016
- 2016-07-28 CN CN201620801386.0U patent/CN206127331U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047705A (en) * | 2016-07-28 | 2016-10-26 | 江南大学 | Mixing device of cell reactor and application thereof |
| CN106047705B (en) * | 2016-07-28 | 2018-09-14 | 江南大学 | A kind of cell reactor mixing arrangement and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170426 Termination date: 20190728 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |