CN106047705A - Mixing device of cell reactor and application thereof - Google Patents
Mixing device of cell reactor and application thereof Download PDFInfo
- Publication number
- CN106047705A CN106047705A CN201610601445.4A CN201610601445A CN106047705A CN 106047705 A CN106047705 A CN 106047705A CN 201610601445 A CN201610601445 A CN 201610601445A CN 106047705 A CN106047705 A CN 106047705A
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- China
- Prior art keywords
- mixing arrangement
- impeller
- cell reactor
- cavity
- reactor mixing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a mixing device of a cell reactor, which can solve the technical problem of mechanical damage to cells in the mixing process. The mixing device comprises an impeller base, wherein the impeller base is provided with a cavity; a lower opening of the cavity is covered by a lower cover plate; a rotary shaft is arranged on the lower cover plate; an impeller is arranged on the rotary shaft, and is arranged in the cavity; a liquid inlet hole is formed in the lower side of the impeller on the impeller base, or the lower cover plate, and the size of the liquid inlet hole is smaller than the size of a carrier.
Description
Technical field
The present invention relates to animal cell culture art field, particularly relate to a kind of cell reactor mixing arrangement and answer
With.
Background technology
The hybrid mode of the commercialization cell reactor of main flow still uses the mode of Mechanical Driven blade stirring at present, and this mixes
Conjunction mode studies maturation, easy and simple to handle.Different from microorganism, due to the acellular wall of zooblast, to shearing extreme sensitivity, no matter
The cell reactor of which kind of form will possess the features such as low sheraing effect, good mixing property.Although cultivating exploitation for cell
Go out low-shearing force blade, but during carrier suspension culture, the carrier being fitted with cell directly can contact with blade, can be to carefully
Born of the same parents cause mechanical damage.
Summary of the invention
For the problems referred to above, the invention provides a kind of cell reactor mixing arrangement, it can solve in mixed process
Cell is caused mechanical damage and the technical problem of mixed effect difference.
Its technical scheme is such, and it includes that impeller base, described impeller base are provided with cavity, the lower ending opening of described cavity
Being covered by lower cover, described lower cover is provided with rotating shaft, and impeller is installed in rotating shaft, is placed in described cavity, described leaf
Wheel seat is positioned on the position on the downside of described impeller or described lower cover is provided with inlet opening, described inlet opening be smaller in size than load
The size of body.
Further, described mixing arrangement also includes that magnetically-actuated motor, described impeller are magnetic impeller or are provided with magnetic
Body, to drive by described magnetically-actuated motor.
Further, described mixing arrangement also includes motor, and described rotating shaft is connected with the output shaft of motor or is motor
Output shaft.
Further, the upper end open of described cavity is covered by upper cover plate, and described upper cover plate is provided with liquid outlet.
Further, described liquid outlet is provided with more than 2；Preferably, described inlet opening is positioned at the diapire of described cavity
Middle part, described liquid outlet is positioned at described impeller and cavity encloses the surface in gap of formation.
Further, described impeller base is columnar structured, described upper cover plate or described lower cover and described impeller base one
Body formed.
Further, bottom described impeller base, it is provided with the fixing support of more than 2, between being provided with between adjacent fixing support
Gap.
Further, described fixing is supported for sucker, alignment pin or locating piece.
Present invention also offers the application in anchorage-dependent cells is cultivated of this mixing arrangement.
The mixing arrangement of the present invention, during use, is placed in cell reactor (or the cell such as fluid reservoir, disposable liquid storing bag
Cultivate associated vessel) bottom or sidewall, impeller rotates, and promotes culture medium in cavity to flow out from upper end open or liquid outlet, with
Time cavity in formation negative pressure so that the inlet opening from bottom of the culture medium outside cavity is sucked so that culture medium is at cell reactor
Internal circulation flow, promotes mixing and gas transfer, the good mixing effect of culture medium；This mixing arrangement operationally, owing to entering
The size being smaller in size than carrier of fluid apertures, only culture medium can be entered in cavity by inlet opening, it is possible to avoid impeller to make cell
Become mechanical damage.
Accompanying drawing explanation
Fig. 1 is the decomposition texture schematic diagram of one embodiment of the invention, wherein eliminates magnetically-actuated motor；
Fig. 2 is the structural representation at another visual angle of impeller base in Fig. 1；
Fig. 3 is the view after the present invention is installed on cell reactor.
Detailed description of the invention
As shown in Fig. 1 ~ Fig. 3, it includes columnar structured impeller base 1, and impeller base 1 is provided with cavity 12, cavity 12 times
End opening is covered by integrated lower cover 13, and lower cover 13 is provided with rotating shaft 14, impeller 3 be installed in rotating shaft 14 and
Being placed in cavity 12, be positioned at below impeller base 1 and be provided with magnetically-actuated motor 2, impeller is magnetic impeller or is provided with magnet, with
Driven by magnetically-actuated motor；It is provided with inlet opening 15 around rotating shaft 14, the chi being smaller in size than carrier of inlet opening on lower cover
Very little；
The upper end open of cavity is covered by upper cover plate 4, and upper cover plate is tightly connected with cavity upper end open, can by glue company or
Arranging the modes such as sealing ring to realize, upper cover plate is provided with 4 upper liquid outlets 41, and liquid outlet 41 is positioned at impeller and cavity encloses formation
The surface in gap；
It is provided with the locating piece 11 of more than 2 bottom impeller base, between adjacent positioned block, is provided with gap 16.
In Fig. 3,5 is the tank body of cell reactor, and 51 is drain pipe, and 52 is feed tube.
As it is shown on figure 3, during assembling, impeller base by locating piece be plugged in the bottom of cell reactor and lower cover and
Gap is left so that culture medium can enter in the cavity of impeller base from inlet opening between the diapire of cell reactor；During use,
Magnetically-actuated motor drives impeller to rotate, and promotes the culture medium in cavity to flow out, simultaneously in cavity from upper end open or liquid outlet
Formation negative pressure so that the inlet opening from bottom of the culture medium outside cavity is sucked so that culture medium is at cell reactor inner recirculation flow
Dynamic, circulate in directional diagram shown in arrow, to promote mixing and the gas transfer of culture medium.
Claims (10)
1. a cell reactor mixing arrangement, it includes that impeller base, described impeller base are provided with cavity, the lower end of described cavity
Opening is covered by lower cover, and described lower cover is provided with rotating shaft, and impeller is installed in rotating shaft, is placed in described cavity, institute
Stating impeller base to be positioned on the position on the downside of described impeller or described lower cover is provided with inlet opening, the size of described inlet opening is little
Size in carrier.
A kind of cell reactor mixing arrangement the most according to claim 1, it is characterised in that: described mixing arrangement also wraps
Including magnetically-actuated motor, described impeller is magnetic impeller or is provided with magnet, to be driven by described magnetically-actuated motor.
A kind of cell reactor mixing arrangement the most according to claim 1, it is characterised in that: described mixing arrangement also wraps
Including motor, described rotating shaft is connected or is the output shaft of motor with the output shaft of motor.
A kind of cell reactor mixing arrangement the most according to claim 1, it is characterised in that: the upper end of described cavity is opened
Mouth is covered by upper cover plate, and described upper cover plate is provided with liquid outlet.
A kind of cell reactor mixing arrangement the most according to claim 4, it is characterised in that: described liquid outlet is provided with 2
More than individual.
A kind of cell reactor mixing arrangement the most according to claim 4, it is characterised in that: described inlet opening is positioned at institute
Stating the middle part of the diapire of cavity, described liquid outlet is positioned at described impeller and cavity encloses the surface in gap of formation.
A kind of cell reactor mixing arrangement the most according to claim 1, it is characterised in that: described impeller base is cylinder
Shape structure, described upper cover plate or described lower cover are one-body molded with described impeller base.
A kind of cell reactor mixing arrangement the most according to claim 1, it is characterised in that: set bottom described impeller base
There is the fixing support of more than 2, between adjacent fixing support, be provided with gap.
A kind of cell reactor mixing arrangement the most according to claim 8, it is characterised in that: described fixing being supported for is inhaled
Dish, alignment pin or locating piece.
10. the application in anchorage-dependent cells is cultivated of the mixing arrangement as described in arbitrary in claim 1 ~ 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610601445.4A CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610601445.4A CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106047705A true CN106047705A (en) | 2016-10-26 |
| CN106047705B CN106047705B (en) | 2018-09-14 |
Family
ID=57417967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610601445.4A Active CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Country Status (1)
| Country | Link |
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| CN (1) | CN106047705B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130751A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Continuous bottling and culturing device of plant cell stirring type bioreactor |
| CN101974408A (en) * | 2010-11-08 | 2011-02-16 | 北京理工大学 | Membrane-type centrifugal rotating shaft bioreactor under microgravity environment |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN206127331U (en) * | 2016-07-28 | 2017-04-26 | 江南大学 | Mixing arrangement for cell reactor |
-
2016
- 2016-07-28 CN CN201610601445.4A patent/CN106047705B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130751A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Continuous bottling and culturing device of plant cell stirring type bioreactor |
| CN101974408A (en) * | 2010-11-08 | 2011-02-16 | 北京理工大学 | Membrane-type centrifugal rotating shaft bioreactor under microgravity environment |
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN206127331U (en) * | 2016-07-28 | 2017-04-26 | 江南大学 | Mixing arrangement for cell reactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106047705B (en) | 2018-09-14 |
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