CN106047705B - A kind of cell reactor mixing arrangement and its application - Google Patents
A kind of cell reactor mixing arrangement and its application Download PDFInfo
- Publication number
- CN106047705B CN106047705B CN201610601445.4A CN201610601445A CN106047705B CN 106047705 B CN106047705 B CN 106047705B CN 201610601445 A CN201610601445 A CN 201610601445A CN 106047705 B CN106047705 B CN 106047705B
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- impeller
- cavity
- mixing arrangement
- cell reactor
- lower cover
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention provides a kind of cell reactor mixing arrangements, the technical issues of capable of solving to cause mechanical damage to cell in mixed process.It includes impeller base, impeller base is equipped with cavity, the lower ending opening of cavity is closed by lower cover lid, lower cover is equipped with shaft, impeller is installed in shaft, is placed in cavity, impeller base is located on the position on the downside of impeller or lower cover is equipped with inlet opening, and the size of inlet opening is less than the size of carrier.
Description
Technical field
The present invention relates to animal cell culture art field more particularly to a kind of cell reactor mixing arrangement and its answer
With.
Background technology
For the hybrid mode of the commercialization cell reactor of mainstream still by the way of Mechanical Driven blade stirring, this is mixed at present
Conjunction mode studies maturation, easy to operate.It is different from microorganism, due to the acellular wall of zooblast, to shearing extreme sensitivity, no matter
The cell reactor of which kind of form will have the features such as low sheraing effect, good mixing property.Although being developed for cell culture
Go out low-shearing force blade, but in carrier suspension incubation, being fitted with the carrier of cell can be in direct contact with blade, can be to thin
Born of the same parents cause mechanical damage.
Invention content
In view of the above-mentioned problems, the present invention provides a kind of cell reactor mixing arrangement, can solve in mixed process
The technical problem of mechanical damage and mixed effect difference is caused to cell.
Its technical solution is such comprising impeller base, the impeller base are equipped with cavity, the lower ending opening of the cavity
It is closed by lower cover lid, the lower cover is equipped with shaft, and impeller is installed in shaft, is placed in the cavity, the leaf
Wheel seat is located on the position on the downside of the impeller or the lower cover is equipped with inlet opening, and the size of the inlet opening, which is less than, to be carried
The size of body.
Further, the mixing arrangement further includes magnetic drive motor, and the impeller is magnetic impeller or is equipped with magnetic
Body, to be driven by the magnetic drive motor.
Further, the mixing arrangement further includes motor, the output axis connection of the shaft and motor or be motor
Output shaft.
Further, the upper end opening of the cavity is closed by upper cover plate lid, and the upper cover plate is equipped with fluid hole.
Further, the fluid hole is equipped with 2 or more；Preferably, the inlet opening is located at the bottom wall of the cavity
Intermediate position, the fluid hole is located at the surface in the gap that the impeller and cavity are enclosed.
Further, the impeller base is columnar structured, the upper cover plate or the lower cover and the impeller base one
It is body formed.
Further, the impeller base bottom is equipped with 2 or more fixed supports, between being equipped between adjacent fixed support
Gap.
Further, the fixed support is sucker, positioning pin or locating piece.
The present invention also provides application of the mixing arrangement in anchorage-dependent cells culture.
The mixing arrangement of the present invention, in use, being placed in cell reactor（Or the cells such as fluid reservoir, disposable liquid storing bag
Cultivate associated vessel）Bottom or side wall, impeller rotation promote the culture medium in cavity to be flowed out from upper end opening or fluid hole, together
When cavity in formation negative pressure the culture medium outside cavity to be sucked from the inlet opening of bottom so that culture medium is in cell reactor
Internal circulation flow promotes mixing and the gas transfer of culture medium, good mixing effect；The mixing arrangement at work, due into
The size of fluid apertures is less than the size of carrier, and only culture medium can be entered by inlet opening in cavity, impeller can be avoided to make cell
At mechanical damage.
Description of the drawings
Fig. 1 is the decomposition texture schematic diagram of one embodiment of the invention, wherein magnetic drive motor is omitted；
Fig. 2 is the structural schematic diagram at another visual angle of impeller base in Fig. 1；
Fig. 3 is that the present invention is installed on the status diagram after cell reactor.
Specific implementation mode
As shown in Fig. 1 ~ Fig. 3 comprising columnar structured impeller base 1, impeller base 1 is equipped with cavity 12, under cavity 12
End opening is covered by integrally formed lower cover 13 and is closed, and lower cover 13 is equipped with shaft 14, and impeller 3 is installed in shaft 14, simultaneously
It being placed in cavity 12,1 lower section of impeller base is provided with magnetic drive motor 2, impeller is magnetic impeller or is equipped with magnet, with
It is driven by magnetic drive motor；It is equipped with inlet opening 15 around shaft 14 on lower cover, the size of inlet opening is less than the ruler of carrier
It is very little；
The upper end opening of cavity is covered by upper cover plate 4 and is closed, and upper cover plate is tightly connected with cavity upper end opening, can be connected by glue
Or the modes such as setting sealing ring are realized, upper cover plate is equipped with 4 upper fluid holes 41, and fluid hole 41 is located at impeller and cavity encloses
The surface in the gap of formation；
Impeller base bottom is equipped with 2 or more locating pieces 11, and gap 16 is equipped between adjacent positioned block.
In Fig. 35 be cell reactor tank body, 51 be outlet tube, and 52 be inlet tube.
As shown in figure 3, assembly when, impeller base by locating piece be plugged in cell reactor bottom and lower cover with
There are gaps between the bottom wall of cell reactor so that culture medium can be from the cavity that inlet opening enters impeller base；In use,
Magnetic drive motor drives impeller rotation, promotes the culture medium in cavity to be flowed out from upper end opening or fluid hole, while in cavity
Formation negative pressure the culture medium outside cavity to be sucked from the inlet opening of bottom so that culture medium is in cell reactor inner recirculation flow
It is dynamic, it circulates in directional diagram shown in arrow, to promote mixing and the gas transfer of culture medium.
Claims (7)
1. a kind of cell reactor mixing arrangement comprising impeller base, the impeller base are equipped with cavity, the lower end of the cavity
Opening is closed by lower cover lid, and the lower cover is equipped with shaft, and impeller is installed in shaft, is placed in the cavity, institute
It states on the position that impeller base is located on the downside of the impeller or the lower cover is equipped with inlet opening, the size of the inlet opening is small
In the size of carrier；
The mixing arrangement further includes magnetic drive motor, and the impeller is magnetic impeller or is equipped with magnet, described to pass through
Magnetic drive motor drives；
The upper end opening of the cavity is closed by upper cover plate lid, and the upper cover plate is equipped with fluid hole.
2. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The fluid hole is equipped with 2
More than a.
3. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The inlet opening is located at institute
The intermediate position of the bottom wall of cavity is stated, the fluid hole is located at the surface in the gap that the impeller and cavity are enclosed.
4. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The impeller base is cylinder
Shape structure, the upper cover plate or the lower cover are integrally formed with the impeller base.
5. a kind of cell reactor mixing arrangement according to claim 1, it is characterised in that：The impeller base bottom is set
There are 2 or more fixed supports, gap is equipped between adjacent fixed support.
6. a kind of cell reactor mixing arrangement according to claim 5, it is characterised in that：The fixed support is suction
Disk, positioning pin or locating piece.
7. application of the mixing arrangement in anchorage-dependent cells culture as described in any in claim 1 ~ 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610601445.4A CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610601445.4A CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106047705A CN106047705A (en) | 2016-10-26 |
| CN106047705B true CN106047705B (en) | 2018-09-14 |
Family
ID=57417967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610601445.4A Active CN106047705B (en) | 2016-07-28 | 2016-07-28 | A kind of cell reactor mixing arrangement and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106047705B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130751A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Continuous bottling and culturing device of plant cell stirring type bioreactor |
| CN101974408A (en) * | 2010-11-08 | 2011-02-16 | 北京理工大学 | Membrane-type centrifugal rotating shaft bioreactor under microgravity environment |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN206127331U (en) * | 2016-07-28 | 2017-04-26 | 江南大学 | Mixing arrangement for cell reactor |
-
2016
- 2016-07-28 CN CN201610601445.4A patent/CN106047705B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101130751A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Continuous bottling and culturing device of plant cell stirring type bioreactor |
| CN101974408A (en) * | 2010-11-08 | 2011-02-16 | 北京理工大学 | Membrane-type centrifugal rotating shaft bioreactor under microgravity environment |
| CN104031824A (en) * | 2013-03-05 | 2014-09-10 | 上海山富科学仪器有限公司 | Stirrer |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN206127331U (en) * | 2016-07-28 | 2017-04-26 | 江南大学 | Mixing arrangement for cell reactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106047705A (en) | 2016-10-26 |
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