CN215887065U - Circulation tangential flow perfusion cell culture system - Google Patents
Circulation tangential flow perfusion cell culture system Download PDFInfo
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- CN215887065U CN215887065U CN202120596698.3U CN202120596698U CN215887065U CN 215887065 U CN215887065 U CN 215887065U CN 202120596698 U CN202120596698 U CN 202120596698U CN 215887065 U CN215887065 U CN 215887065U
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- 230000010412 perfusion Effects 0.000 title claims abstract description 37
- 238000004113 cell culture Methods 0.000 title claims abstract description 13
- 238000003756 stirring Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 7
- 230000005540 biological transmission Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000010008 shearing Methods 0.000 abstract description 14
- 239000000463 material Substances 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000014759 maintenance of location Effects 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 description 10
- 230000002572 peristaltic effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000011148 porous material Substances 0.000 description 3
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000010924 continuous production Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 238000005429 filling process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000030833 cell death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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Abstract
The utility model discloses a circulating tangential flow perfusion cell culture system which comprises a bottom plate, wherein two supporting rods are connected to one side of the top of the bottom plate, and a circulating pump is fixedly arranged on one side, far away from the supporting rods, of the top of the bottom plate. The utility model can circulate the cells back to the bioreactor body in a circulating mode, thereby ensuring that the cells in the perfusion system and the cells in the bioreactor body are always exchanged, solving the problem of cell retention, customizing a circulating pump with low shearing force, ensuring that the cells are not influenced by overlarge shearing force, ensuring that the materials in the perfusion system and the materials in the bioreactor body are always circulated, ensuring that the materials inside and outside the bioreactor body are consistent, and the circulating pump has no pulse, no contact, no friction, stable and no fluctuation of flow, long perfusion time, can effectively reduce the production cost, prolongs the single-batch culture time, also reduces the production cost, brings more benefits to enterprises, and the whole perfusion device has simple structure, small floor area and convenient operation.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a circulating tangential flow perfusion cell culture system.
Background
With the gradual progress of production technology, the pharmaceutical industry has begun to explore the continuous production instead of batch production, the continuous production refers to 24-hour continuous operation, and is a new technology compared with batch production, so that the production process of the medicine can be really more reliable and efficient, and perfusion culture is the most effective continuous culture mode of cell culture.
The prior art has the following defects: 1. the perfusion culture mode popular in the existing market is an alternate tangential flow mode, an alternate tangential flow perfusion culture system is driven by an alternate diaphragm pump, cells pumped in the culture process cannot effectively return to a bioreactor, the titer in the perfusion system is far higher than that in the reactor, and membrane holes are easy to block, so that perfusion is finished in advance; 2. a small amount of peristaltic pump circulating perfusion systems are used in the market, and a large amount of cells die due to large shearing force of the peristaltic pumps, so that membrane pores are blocked quickly, and perfusion cannot be performed; 3. peristaltic pumps and diaphragm pumps in the prior art have large pulses, so that the flow rate of liquid is unstable, and a diaphragm column is easy to block; 4. the working principle of the peristaltic pump is an extrusion design, so that the silicone tube is easy to break, the culture is interrupted, and the loss is caused; 5. the existing alternative tangential flow perfusion device has the disadvantages of complex power system, alternative use of a compression pump and a vacuum pump, high energy consumption and high risk.
The above information disclosed in this background section is only for enhancement of understanding of the background of the disclosure and therefore it may contain information that does not constitute prior art that is already known to a person of ordinary skill in the art.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a circulating tangential flow perfusion cell culture system, which can circulate cells back to a bioreactor body in a circulating mode, thereby ensuring that the cells in the perfusion system and the cells in the reactor body are exchanged all the time, solving the problem of cell retention, customizing a circulating pump with low shearing force, ensuring that the cells are not influenced by overlarge shearing force, ensuring that the materials in the perfusion system and the reactor body are circulated all the time in the perfusion process, ensuring that the materials inside and outside the reactor body are consistent, and ensuring that the circulating pump has no pulse, no contact, no friction, stable and no fluctuation of flow and long perfusion time, can effectively reduce production cost, prolong single batch culture time, reduce production cost equally, bring more benefits for the enterprise, whole perfusion device simple structure, area is very little, convenient operation to solve the problem among the above-mentioned background art.
In order to achieve the above purpose, the utility model provides the following technical scheme: the utility model provides a circulation tangential flow fills cell culture system, includes the bottom plate, bottom plate top one side is connected with two bracing pieces, the fixed circulating pump that is provided with in one side that the bracing piece was kept away from at the bottom plate top, two the bracing piece top is connected with the reactor body, the fixed motor that is provided with in reactor body top, motor output shaft end transmission is connected with the (mixing) shaft, the fixed stirring rake that is provided with in (mixing) shaft bottom, be connected with the pipette between circulating pump and the reactor body, the circulating pump top is provided with the filter, be connected with the connecting pipe between filter and the circulating pump, be connected with the fluid-discharge tube between filter and the reactor body.
Preferably, the paddle is arranged like an ear.
Preferably, one side of the top of the reactor body, which is far away from the circulating pump, is connected with an air inlet pipe in a penetrating way.
In the technical scheme, the utility model provides the following technical effects and advantages:
make the cell can circulate back to the bioreactor body through the endless mode, thereby guarantee that the cell in the filling system has the exchange with this internal cell of reactor all the time, prevent that the cell from detaining in filling device, the cell detention problem has been solved, customize the circulating pump of low shearing force, guarantee that the cell is not influenced by too big shearing force, material in the filling system all the time and this internal circulation of reactor among the filling process, guarantee that the internal and external material of reactor is unanimous, the circulating pump is pulseless, contactless, no friction, the flow stability is undulant, the perfusion time is long, can effectively reduce the time and the consumptive material cost of changing diaphragm etc., reduce manufacturing cost, the extension of single batch culture time, thereby reduce manufacturing cost, can bring more benefits for the enterprise, whole perfusion device simple structure, floor area is very little, and convenient for operation.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.
Fig. 1 is a schematic view of the overall structure of the present invention.
Description of reference numerals:
1. a base plate; 2. a support bar; 3. a circulation pump; 4. a reactor body; 5. a motor; 6. a stirring shaft; 7. a stirring paddle; 8. a pipette; 9. a filter; 10. a connecting pipe; 11. a liquid discharge pipe; 12. an air inlet pipe; 13. CPC sterile connector.
Detailed Description
Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the examples set forth herein; rather, these example embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The drawings are merely schematic illustrations of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and thus their repetitive description will be omitted.
Furthermore, the described features, structures, or characteristics may be combined in any suitable manner in one or more example embodiments. In the following description, numerous specific details are provided to give a thorough understanding of example embodiments of the disclosure. One skilled in the relevant art will recognize, however, that the subject matter of the present disclosure can be practiced without one or more of the specific details, or with other methods, components, steps, and so forth. In other instances, well-known structures, methods, implementations, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.
The utility model provides a circulating tangential flow perfusion cell culture system as shown in figure 1, which comprises a bottom plate 1, wherein one side of the top of the bottom plate 1 is connected with two support rods 2, one side of the top of the bottom plate 1, which is far away from the support rods 2, is fixedly provided with a circulating pump 3, the tops of the two support rods 2 are connected with a reactor body 4, the top of the reactor body 4 is fixedly provided with a motor 5, the end part of an output shaft of the motor 5 is in transmission connection with a stirring shaft 6, the bottom of the stirring shaft 6 is fixedly provided with a stirring paddle 7, a liquid suction pipe 8 is connected between the circulating pump 3 and the reactor body 4, the top of the circulating pump 3 is provided with a filter 9, a connecting pipe 10 is connected between the filter 9 and the circulating pump 3, and a liquid discharge pipe 11 is connected between the filter 9 and the reactor body 4.
Further, in the above technical scheme, the stirring paddle 7 is arranged like an ear, and the stirring paddle 7 is used for stirring the cells, so that the cells and the culture solution are fully mixed.
Further, in the above technical solution, an air inlet pipe 12 is connected to one side of the top of the reactor body 4, which is far away from the circulation pump 3, and air is introduced into the reactor body 4 during culture to induce cell culture.
The implementation mode is specifically as follows: the motor 5 and the circulating pump 3 are both connected with an external power supply through leads, when the reactor works, the motor 5 is connected to drive the stirring shaft 6 and the stirring paddle 7 to rotate to stir the materials in the reactor body 4, the circulating pump 3 provides power to continuously pump the materials in the reactor body 4 out and then return to the reactor body 4 from the top, so that a complete one-in one-out circulation passage is formed to replace an alternate in-out passage of alternate tangential flow, the circulating pump 3 can ensure high flow velocity under the condition of low shearing force and can ensure sufficient tangential flow velocity to effectively scour the surface of a membrane and prevent the membrane column from being blocked, the circulating pump 3 is a fixed plate making and is specially designed for fluid application, the flow rate is ultralow in shearing force, has no pulse and can be continuously controlled, and the circulating pump 3 is based on the magnetic suspension principle, an impeller is suspended in a sealed cavity and is not in contact and is driven by a magnetic field, so that the almost 0 shearing force is realized, the shearing force is the root cause of cell death and membrane column blockage, the circulating pump 3 has no bearing and sealing ring, no friction and sealing ring damage exist in the operation process, the continuous operation time is long, the flow rate is stable and reliable, the pump head has no friction, the service time is long, the time and material consumption cost for replacing a membrane and the like can be effectively reduced, the production cost is reduced, the risk caused by replacing the membrane and the membrane column is reduced, the floor area of the circulating pump 3 is extremely small, power can be provided only by supplying power, the structure is simple, and the operation is convenient, the embodiment specifically solves the problems that the existing perfusion culture mode popular in the market at present in the prior art is an alternate tangential flow mode, an alternate tangential flow perfusion culture system is driven by an alternate diaphragm pump, cells pumped in the culture process cannot effectively return to a bioreactor, the titer in the perfusion system is far higher than that in the reactor, and membrane pores are easy to block, leading to premature end of perfusion; a small amount of peristaltic pump circulating perfusion systems are used in the market, and a large amount of cells die due to large shearing force of the peristaltic pumps, so that membrane pores are blocked quickly, and perfusion cannot be performed; peristaltic pumps and diaphragm pumps in the prior art have large pulses, so that the flow rate of liquid is unstable, and a diaphragm column is easy to block; the working principle of the peristaltic pump is an extrusion design, so that the silicone tube is easy to break, the culture is interrupted, and the loss is caused; the existing alternative tangential flow perfusion device has the problems of complex power system, alternative use of a compression pump and a vacuum pump, high energy consumption and high risk.
This practical theory of operation: make the cell can circulate back to bioreactor body 4 through the endless mode, thereby guarantee that the cell in the filling system has the exchange with the cell in the reactor body 4 all the time, the cell retention problem has been solved, customize circulating pump 3 of low shearing force, guarantee that the cell does not receive the influence of too big shearing force, material in the filling system all the time and reactor body 4 internal circulation in the filling process, guarantee that the inside and outside material of reactor body 4 is unanimous, circulating pump 3 does not have the pulse, contactless, no friction, the flow stability is undulant, the perfusion time is long, can effectively reduce manufacturing cost, the extension of single batch culture time, same reduction in production cost, bring more benefits for the enterprise, whole perfusion device simple structure, floor area is very little, and convenient operation.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the utility model.
Claims (3)
1. A circulating tangential flow perfusion cell culture system, comprising a bottom plate (1), characterized in that: the improved reactor is characterized in that two support rods (2) are connected to one side of the top of the bottom plate (1), a circulating pump (3) and two circulating pumps are fixedly arranged on one side, far away from the support rods (2), of the top of the bottom plate (1), a reactor body (4) is connected to the top of the support rods (2), a motor (5) is fixedly arranged on the top of the reactor body (4), a stirring shaft (6) is connected to the end portion of an output shaft of the motor (5) in a transmission mode, stirring paddles (7) are fixedly arranged at the bottom of the stirring shaft (6), a liquid suction pipe (8) is connected between the circulating pump (3) and the reactor body (4), a filter (9) is arranged on the top of the circulating pump (3), a connecting pipe (10) is connected between the filter (9) and the circulating pump (3), and a liquid discharge pipe (11) is connected between the filter (9) and the reactor body (4).
2. A circulating tangential flow perfusion cell culture system as claimed in claim 1, wherein: the stirring paddle (7) is arranged like a lug.
3. A circulating tangential flow perfusion cell culture system as claimed in claim 1, wherein: and an air inlet pipe (12) is connected to one side of the top of the reactor body (4) far away from the circulating pump (3) in a penetrating manner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120596698.3U CN215887065U (en) | 2021-03-24 | 2021-03-24 | Circulation tangential flow perfusion cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120596698.3U CN215887065U (en) | 2021-03-24 | 2021-03-24 | Circulation tangential flow perfusion cell culture system |
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| Publication Number | Publication Date |
|---|---|
| CN215887065U true CN215887065U (en) | 2022-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202120596698.3U Active CN215887065U (en) | 2021-03-24 | 2021-03-24 | Circulation tangential flow perfusion cell culture system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116004389A (en) * | 2022-04-21 | 2023-04-25 | 泰舸赛迩生命科技(无锡)有限公司 | Low-shear force pulsation-free liquid circulation device and method for biomedical production |
-
2021
- 2021-03-24 CN CN202120596698.3U patent/CN215887065U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116004389A (en) * | 2022-04-21 | 2023-04-25 | 泰舸赛迩生命科技(无锡)有限公司 | Low-shear force pulsation-free liquid circulation device and method for biomedical production |
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Effective date of registration: 20240103 Address after: No.500, Xincheng Road, Jiading District, Shanghai Patentee after: Shanghai Jichen Biotechnology Co.,Ltd. Address before: 050000 room 1612, building 1, No. 136, Huanghe Avenue, high tech Zone, Shijiazhuang City, Hebei Province Patentee before: HEBEI SANTA JICHEN BIO-TECHNOLOGY Co.,Ltd. |