CN213416915U

CN213416915U - Stem cell culture device

Info

Publication number: CN213416915U
Application number: CN202022240062.3U
Authority: CN
Inventors: 王世荣
Original assignee: Shandong Huahang Medical Technology Co Ltd
Current assignee: Shandong Huahang Medical Technology Co Ltd
Priority date: 2020-10-10
Filing date: 2020-10-10
Publication date: 2021-06-11
Anticipated expiration: 2030-10-10

Abstract

The utility model belongs to the technical field of stem cell culture, concretely relates to stem cell culture device, which comprises a main frame, one side of the main frame is provided with an incubator, the upside of the incubator is provided with a liquid storage tank, the upside of the main frame is provided with a preparation tank, a lower discharge port of the preparation tank is communicated with the liquid storage tank through a valve, the inner side of the main frame is provided with a supplementary pump, a liquid suction port of the supplementary pump is communicated with the liquid storage tank, a liquid discharge port of the supplementary pump is connected with the incubator, the inner part of the incubator is provided with a culture tank, the inner side of the culture tank is provided with a liquid level sensor, and the inner wall of the incubator is provided with; the utility model discloses a liquid reserve tank, supplementary pump, supplementary pipe, drip irrigation head and level sensor of design, it is how much through the culture solution of level sensor response culture tank when using, then supply in dripping into the culture tank to the culture solution through driping irrigation the head to make and keep sufficient culture solution in the culture tank always, make the stem cell more stable when cultivateing.

Description

Stem cell culture device

Technical Field

The utility model relates to a stem cell culture technical field specifically is a stem cell culture device.

Background

The function of the stem cell population is to control and maintain the regeneration of cells. Generally, between stem cells and their terminally differentiated progeny, there is an intermediate population of progenitor cells, called "committed progenitors," which have limited expansion capacity and limited differentiation potential. These cell populations function to increase the number of differentiated cells produced after each division of the stem cells. Stem cells have the ability to self-renew, but stem cell division is in fact relatively asymmetric.

When the conventional stem cell culture device is used, culture solution needs to be supplemented for long-time culture, and the phenomenon that the culture solution cannot be supplemented in time can occur due to the fact that observation cannot be carried out for a long time, so that the stem cell culture device is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a stem cell culture device has solved a current stem cell culture device when using, and long-time cultivation needs supply the culture solution, can appear the problem that can not in time supply the culture solution owing to can not observe for a long time.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a stem cell culture device, includes the body frame, one side of body frame is provided with the incubator, the upside of incubator is provided with the liquid reserve tank, the upside of body frame is provided with the preparation jar, the lower extreme bin outlet of preparing the jar passes through valve and liquid reserve tank intercommunication, the inboard of body frame is provided with the supplementary pump, the drawing liquid mouth and the liquid reserve tank intercommunication of supplementary pump, the leakage fluid dram and the incubator of supplementary pump are connected, the inside of incubator is provided with the culture tank, the inboard of culture tank is provided with level sensor, the inner wall of incubator is provided with the additional tube, the one end of additional tube is connected with the leakage fluid dram of supplementary pump, the downside of additional tube is provided with drips irrigation the head.

Preferably, the upper end of preparation jar is provided with the drive case, upper end one side of preparation jar is provided with the charge door, the upside of drive case is provided with agitator motor, the inside of preparation jar is provided with the main shaft, the both ends fixedly connected with rolling disc of main shaft, the both ends of main shaft are passed through rolling disc and bearing and are rotated with drive case and preparation jar respectively and be connected, and the upper end of main shaft and agitator motor's output shaft fixed connection, the edge of rolling disc is provided with the driven shaft.

Preferably, the upper end fixedly connected with gear of driven shaft, the inner wall fixedly connected with ring gear of drive case, the ring gear passes through tooth meshing with the gear and is connected.

Preferably, the surfaces of the main shaft and the driven shaft are provided with stirring blades, and the stirring blades on the main shaft and the driven shaft are installed in a staggered mode.

Preferably, the supplementing pipe is installed in the incubator in an S shape, the incubator is fixed on the inner wall of the incubator through bolts, and the drip irrigation heads on the lower side of the supplementing pipe correspond to the incubator in position one by one.

Preferably, the driven shaft is installed on the rotating disc in a central symmetry manner by taking the center of the main shaft as a center, and the driven shaft is rotatably connected with the rotating disc through a bearing.

Preferably, the inside of liquid reserve tank is provided with the filter screen, and the inboard bottom of liquid reserve tank sets up to the inclined plane.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses a liquid reserve tank, supplementary pump, supplementary pipe, drip irrigation head and level sensor of design, it is how much through the culture solution of level sensor response culture tank when using, then supply in dripping into the culture tank to the culture solution through driping irrigation the head to make and keep sufficient culture solution in the culture tank always, make the stem cell more stable when cultivateing.

2. The utility model discloses a ring gear, driven shaft and the main shaft of design process the culture solution when the use device, can rotate through agitator motor drive main shaft and rolling disc to drive the driven shaft through the transmission of ring gear and rotate, make the driven shaft carry out the rotation when around main shaft pivoted, thereby better carry out the rapid mixing configuration to the cultivation, can be timely to supplying the culture solution in the liquid reserve tank.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic diagram of the internal structure of the utility model at A in FIG. 1;

fig. 3 is a schematic view of the structure inside the incubator of the present invention.

In the figure: 1. a main frame; 2. preparing a tank; 3. a drive box; 4. a stirring motor; 5. a feed inlet; 6. a liquid storage tank; 7. an incubator; 8. a main shaft; 9. a driven shaft; 10. rotating the disc; 11. a ring gear; 12. a gear; 13. a replenishing pipe; 14. a drip irrigation head; 15. a culture tank; 16. a liquid level sensor; 17. the pump is replenished.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Examples

Referring to fig. 1-3, a stem cell culture apparatus comprises a main frame 1, wherein one side of the main frame 1 is provided with an incubator 7, the upper side of the incubator 7 is provided with a liquid storage tank 6, the upper side of the main frame 1 is provided with a preparation tank 2, a discharge port at the lower end of the preparation tank 2 is communicated with the liquid storage tank 6 through a valve, the inner side of the main frame 1 is provided with a supplementary pump 17, a liquid suction port of the supplementary pump 17 is communicated with the liquid storage tank 6, a liquid discharge port of the supplementary pump 17 is connected with the incubator 7, the inner part of the incubator 7 is provided with an incubator 15, the inner side of the incubator 15 is provided with a liquid level sensor 16, the inner wall of the incubator 7 is provided with a supplementary pipe 13, one end of the supplementary pipe 13 is connected with the liquid discharge port of the supplementary pump 17, the lower side of the supplementary pipe 13 is provided with a drip irrigation head 14, the amount of culture liquid in the incubator 15 is sensed through the liquid level sensor 16, the culture solution is supplemented into the culture tank 15 lacking the culture solution through the drip irrigation head 14, so that the culture solution is kept sufficient, and the stem cells are more stable during culture.

Referring to fig. 1-2, a driving box 3 is disposed at an upper end of a preparation tank 2, a charging opening 5 is disposed at one side of the upper end of the preparation tank 2, a stirring motor 4 is disposed at an upper side of the driving box 3, a main shaft 8 is disposed inside the preparation tank 2, rotating discs 10 are fixedly connected to two ends of the main shaft 8, two ends of the main shaft 8 are rotatably connected to the driving box 3 and the preparation tank 2 through the rotating discs 10 and bearings, respectively, the upper end of the main shaft 8 is fixedly connected to an output shaft of the stirring motor 4, a driven shaft 9 is disposed at an edge of the rotating disc 10, the main shaft 8 is driven by the stirring motor 4 to rotate, the main shaft 8 drives the rotating discs 10 to rotate, the rotating discs 10 drive the driven shaft 9 to revolve around the main shaft 8, and a gear 12 moves along a gear ring 11 and drives the.

Referring to fig. 2, a gear 12 is fixedly connected to the upper end of the driven shaft 9, a gear ring 11 is fixedly connected to the inner wall of the driving box 3, and the gear ring 11 is engaged with the gear 12 through teeth.

Referring to fig. 2, the surfaces of the main shaft 8 and the driven shaft 9 are both provided with stirring blades, and the stirring blades on the main shaft 8 and the driven shaft 9 are installed in a staggered manner, so that the main shaft 8 and the driven shaft 9 have better stirring effect when in use.

Referring to fig. 3, the supplement pipe 13 is installed inside the cultivation box 7 in an S-shape, the cultivation tank 15 is fixed on the inner wall of the cultivation box 7 by bolts, and the drip irrigation heads 14 at the lower side of the supplement pipe 13 correspond to the cultivation tank 15 in one-to-one position, so that the supplement pipe 13 and the drip irrigation heads 14 can supplement the cultivation tank 15 with the culture solution conveniently.

Referring to fig. 2, the driven shaft 9 is installed on the rotating disc 10 in a central symmetry manner around the center of the main shaft 8, and the driven shaft 9 is rotatably connected with the rotating disc 10 through a bearing, so that the stirring motor 4 can drive the main shaft 8 to rotate, and the driven shaft 9 is driven to rotate along with the matching.

Referring to fig. 1, a filter screen is disposed inside the liquid storage tank 6, and the bottom of the inner side of the liquid storage tank 6 is an inclined surface, so that the liquid in the liquid storage tank 6 can be conveniently pumped by the supplementary pump 17.

The utility model discloses the concrete implementation process as follows: when in use, stem cells are cultured in the culture tank 15, the amount of the culture solution in the culture tank 15 is sensed by the liquid level sensor 16, then the culture solution is pumped from the liquid storage tank 6 into the supplement pipe 13 through the supplement pump 17, the culture solution is supplemented into the culture tank 15 lacking the culture solution through the drip irrigation head 14, so that the culture solution is kept sufficient, the stem cells are more stable during culture, when the culture solution in the liquid storage tank 6 is short, the ingredients are added into the preparation tank 2 through the feed inlet 5, then the main shaft 8 is driven to rotate through the stirring motor 4, the main shaft 8 drives the rotating disc 10 to rotate, so that the rotating disc 10 drives the driven shaft 9 to revolve around the main shaft 8, simultaneously gear 12 removes along ring gear 11 to meshing through the tooth makes gear 12 drive the rotation of driven shaft 9, makes main shaft 8 and driven shaft 9 mutually support and carry out the rapid mixing configuration to the batching, thereby can be fast to replenishing the culture solution in the liquid reserve tank 6.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A stem cell culture device, comprising a main frame (1), characterized in that: an incubator (7) is arranged on one side of the main frame (1), a liquid storage tank (6) is arranged on the upper side of the incubator (7), a preparation tank (2) is arranged on the upper side of the main frame (1), a discharge hole at the lower end of the preparation tank (2) is communicated with a liquid storage tank (6) through a valve, a supplementary pump (17) is arranged on the inner side of the main frame (1), a liquid pumping port of the supplementary pump (17) is communicated with the liquid storage tank (6), a liquid outlet of the supplementary pump (17) is connected with the incubator (7), the incubator (7) is internally provided with an incubator (15), a liquid level sensor (16) is arranged on the inner side of the culture tank (15), a replenishing pipe (13) is arranged on the inner wall of the culture box (7), one end of the supplementary pipe (13) is connected with a liquid outlet of a supplementary pump (17), and a drip irrigation head (14) is arranged on the lower side of the supplementary pipe (13).

2. A stem cell culture apparatus according to claim 1, wherein: the upper end of preparation jar (2) is provided with drive case (3), upper end one side of preparation jar (2) is provided with charge door (5), the upside of drive case (3) is provided with agitator motor (4), the inside of preparation jar (2) is provided with main shaft (8), the both ends fixedly connected with rolling disc (10) of main shaft (8), the both ends of main shaft (8) are passed through rolling disc (10) and bearing and are rotated with drive case (3) and preparation jar (2) respectively and be connected, and the upper end of main shaft (8) and the output shaft fixed connection of agitator motor (4), the edge of rolling disc (10) is provided with driven shaft (9).

3. A stem cell culture apparatus according to claim 2, wherein: the upper end fixedly connected with gear (12) of driven shaft (9), the inner wall fixedly connected with ring gear (11) of drive case (3), ring gear (11) are connected through tooth meshing with gear (12).

4. A stem cell culture apparatus according to claim 2, wherein: stirring blades are arranged on the surfaces of the main shaft (8) and the driven shaft (9), and the stirring blades on the main shaft (8) and the driven shaft (9) are installed in a staggered mode.

5. A stem cell culture apparatus according to claim 1, wherein: the supplementing pipe (13) is installed in the incubator (7) in an S shape, the incubator (15) is fixed on the inner wall of the incubator (7) through bolts, and the drip irrigation heads (14) on the lower side of the supplementing pipe (13) correspond to the positions of the incubator (15) one by one.

6. A stem cell culture apparatus according to claim 2, wherein: the driven shaft (9) is installed on the rotating disc (10) in a central symmetry mode by the position center of the main shaft (8), and the driven shaft (9) is rotatably connected with the rotating disc (10) through a bearing.

7. A stem cell culture apparatus according to claim 1, wherein: the inside of liquid reserve tank (6) is provided with the filter screen, and the inboard bottom of liquid reserve tank (6) sets up to the inclined plane.