A kind of gamma-polyglutamic acid-float bed type reactor
Technical field
The utility model relates to a kind of gamma-polyglutamic acid-production technology apparatus field, particularly a kind of gamma-polyglutamic acid-float bed type reactor.
Background technology
Gamma-polyglutamic acid-(γ-polyglutamic acid, be called for short γ-PGA) be a kind of polyamino acid type biopolymer by microorganisms, it is formed by the γ-carboxyl of a part L-glutamic acid and the alpha-amino group condensation of another molecule L-glutamic acid, more than molecular weight can reach 2000kDa, structural formula is:
Gamma-polyglutamic acid-has physics and chemistry and the biological characteristics of many uniquenesses such as splendid biodegradability, film-forming properties, one-tenth fibering, plasticity-, cohesiveness, moisture retention, focus on environmental protection, emphasizing today of Sustainable development, gamma-polyglutamic acid-and derivative thereof have very wide application prospect, can be used for makeup, fertilizer synergist, food, dispersion agent, sequestrant, building coating, the field such as dustproof.
There are two kinds of modes about the fermentative production of gamma-polyglutamic acid-: free thalline fermentation mode and immobilized thallus fermentation mode, the shortcoming of free thalline fermentation mode is that fermentation time is long, energy consumption is large, yield poorly, production efficiency is low; Immobilized thallus fermentation mode is more suitable for suitability for industrialized production gamma-polyglutamic acid-, can carry out the multiple batches of production of continuously fermenting, and is suitable for gamma-polyglutamic acid-high viscosity viable cell zymotechnique.
Prior art patent of invention CN103194374A, a kind of pillar immobilization reactor and technique thereof of fermentative production gamma-polyglutamic acid-are disclosed, this device is made up of stirring reactor part and aerobic immobilization post part, and two portions are connected and are carried out material exchange by constant flow pump; Shortcoming is that reactor separates with curing thalline, affects overall ferment effect; Industrialization is used complicated thalline fermentation area restricted; Mixing, stirring and the transmission homogeneity of material are undesirable.
Utility model content
For solving the problems of the technologies described above, the utility model provides a kind of gamma-polyglutamic acid-float bed type reactor, for deficiency of the prior art, design a kind of reactor of applicable gamma-polyglutamic acid-polycondensation, immobilized thallus fermentation is combined with the fermentation of free type thalline, adopt thalline float bed type structure, use Rotating Stainless Steel Cage structure fibrous reticulum to be assemblied in the stainless copper cage of different structure, simultaneously, drive cage body to be rotated by motor, promote on the one hand thalline implantation, play on the other hand stirring, mixing effect, by Purge gas oxygenation, reduce energy consumption, improve and mix, the homogeneity of stirring and material transfer, promote overall fermentation yield and efficiency.
For achieving the above object, the technical solution of the utility model is as follows: a kind of gamma-polyglutamic acid-float bed type reactor, comprise tank body, temperature control interlayer, thermal insulation layer, water outlet, head sealing cover, tensimeter, safety valve, constant pressure valve, step-down gear, motor, thermometer, main shaft, Rotating Stainless Steel Cage, upflow tube, reinforced volume pump, pH monitor, circulation feed bin, recycle pump, gas distribution pipe, discharge port, air valve, purification air pump, cold water outlet, hot water mouth, pneumatic panel, plane bacterium bed and ring-type bacterium bed, it is characterized in that:
Described tank body is made up of right cylinder, cone and head sealing cover, and described right cylinder lower end and cone are welded and fixed, and described right cylinder upper end is provided with ring flange, and described ring flange is connected by bolt seal with described head sealing cover, described tank body is provided with temperature control interlayer, and described temperature control interlayer arranged outside has thermal insulation layer, described encapsulation header covers and is provided with constant pressure valve and thermometer, described head sealing cover central position is provided with main shaft, and described main shaft upper end is connected with step-down gear, and described step-down gear is connected with motor, described main shaft is turning axle, and the lower end of described main shaft is connected with the sleeve sealing at pneumatic panel center, described tank body cone bottom is provided with discharge port, the cone inside of described tank body is provided with gas distribution pipe, described tank body lower end and cone intersection are provided with pneumatic panel, described tank interior is provided with Rotating Stainless Steel Cage, described Rotating Stainless Steel Cage central position and main shaft are welded and fixed, described Rotating Stainless Steel Cage is provided with plane bacterium bed and ring-type bacterium bed, top, described tank body side arranges upflow tube, described upflow tube is communicated with described circulation feed bin top, described circulation bin bottom is communicated with recycle pump by pipeline, described tank body cone bottom is provided with cycle stock mouth, described recycle pump is communicated with cycle stock mouth by pipeline, described circulation feed bin top is provided with pH monitor and described reinforced volume pump, and described reinforced volume pump is communicated with (not marking) by pipeline with feeding chamber, described purification air pump and air-purification system (not marking) are passed through pipeline communication.
Described plane bacterium bed at least arranges two layers, and described ring-type bacterium bed at least arranges one deck; The thickness of described plane bacterium bed and ring-type bacterium bed is 50mm-200mm.
Described temperature control interlayer top is provided with water outlet, and the cone part of described temperature control interlayer is provided with hot water mouth and cold water outlet; Described constant pressure valve bypass is provided with safety valve and tensimeter; Described discharge opening pipeline bypass is communicated with air valve and purification air pump.
Described plane bacterium bed and ring-type bacterium bed form by fibrous reticulum is curling, and described fibrous reticulum is fixed by Rotating Stainless Steel Cage, in the time of described main shaft rotation, drive Rotating Stainless Steel Cage together to rotate, and described plane bacterium bed and ring-type bacterium bed are also rotated simultaneously in tank body; Described plane bacterium bed in described kinestate and ring-type bacterium bed, play stirring, mixing effect for fermentation materials, is being uniformly mixed under effect, and to fermented liquid, performance increases the effect of oxygen supply, transmitter substance.
Described plane bacterium bed is the Rotating Stainless Steel Cage structure that circular flat arranges, and described fibrous reticulum is vertically wound in Rotating Stainless Steel Cage; Described ring-type bacterium bed is the Rotating Stainless Steel Cage structure that annular arranges, and described fibrous reticulum is horizontally set in ring stainless steel cage; The Rotating Stainless Steel Cage of described plane bacterium bed and ring-type bacterium bed is welded and fixed each other; The fibrous reticulum arranging in described plane bacterium bed and ring-type bacterium bed, all perpendicular with the tangent line of sense of rotation, the fermentation liquid material body in therefore guaranteeing described fibrous reticulum and flowing fully mixes and stirring.
Described gas distribution pipe is communicated with described discharge port, therefore purifying air of discharge port bypass can be pumped into fermentor tank by purified gas, described gas distribution pipe is at least provided with two, described gas distribution pipe is uniformly distributed along described cone surface angle of inclination in tank body cone, is provided with multiple uniform qi-emitting holes on the tube wall of described gas distribution pipe.
On described pneumatic panel, be provided with uniform air distribution hole.
Between described adjacent plane bacterium bed, distance is 100mm-300mm.
Distance between described adjacent ring-type bacterium bed is 150mm-250mm.
In described tank body, dose gamma-polyglutamic acid-fermented liquid, in described circulation feed bin, store the fermented liquid overflowing, described fermentor tank Flow of Goods and Materials, mix, stir and drive fermented liquid by recycle pump, enter tank body cone part from fermentor tank bottom, and then after uniform by pneumatic panel, enter described plane bacterium bed and ring-type Jun Chuan district, fermented liquid is under recycle pump pressure-driven, successively by plane bacterium bed and ring-type bacterium bed, active ingredient in fermented liquid, can be attached in the reticulin fiber of fiber, between adjacent plane bacterium bed and ring-type bacterium bed, because bacterium bed is in rotating state, fermented feed liquid is subject to the rising motivating force effect of centrifugal force and recycle pump, make the fermented liquid of flowing through be able to fully stir and mix, along with the rising of fermented liquid and the increase of recycle pump mass transfer amount, the fermentation liquid measure of tank interior increases, liquid level raises, in the time that fermented liquid liquid level meets and exceeds upflow tube, fermented liquid can flow in circulation feed bin by upflow tube, enter next round circulation mass transfer process.
Described air, after purifying, is sent in gas distribution pipe by purification system, through gas distribution pipe gas distribution, be uniformly distributed gas, and fully contact with the fermented liquid in tank body cone, under recycle pump transmission fermented liquid flows, further gas mixed with fermented liquid and distribute by pneumatic panel; Described purifying air risen together and enters in plane bacterium bed and ring-type bacterium bed in company with fermented liquid, and by the multiple plane bacterium beds and the ring-type bacterium bed that arrange on Rotating Stainless Steel Cage, for free live flora in the live flora in each bacterium bed and tank body brings oxygen, and then increase the exchange of oxygen and the transmission of fermented liquid exchange, described air is after each layer plane bacterium bed and ring-type bacterium bed, rise to tank body top, and outwards discharge by constant pressure valve intermittent type, guarantee the pressure that tank interior is certain, therefore can promote the oxygen level in fermented liquid.
In described fermentor tank, the temperature of fermented liquid, carries out thermostatic control by hot water and the cold water of heat exchanger; Pressure in described tank body and temperature data detect by tensimeter and thermometer; During the fermentation, the culture base-material body that needs stream to add in fermentation system, quantitatively add by reinforced volume pump, pH value and total residual sugar amount in fermentation system are monitored by circulation feed bin, and regulate by the pH value to circulation feed bin, control the every fermentation parameter in whole fermentation system.
When after the fermented liquid fermentation ends in fermentor tank, by discharge port, fermented liquid is discharged to system, further carry out Separation & Purification, obtain gamma-polyglutamic acid-solution or sterling crystal; And active thalline in fermentation system can be deposited in fiber bacterium bed, dose the fermented liquid of next batch and can proceed gamma-polyglutamic acid-fermentation, thereby can realize multiple batches of continuous production.
Pass through technique scheme, the beneficial effect of technical solutions of the utility model is: immobilized thallus fermentation is combined with the fermentation of free type thalline, adopt thalline float bed type structure, use Rotating Stainless Steel Cage structure fibrous reticulum to be assemblied in the stainless copper cage of different structure, simultaneously, drive cage body to be rotated by motor, promote on the one hand thalline implantation, play on the other hand stirring, mixing effect, by Purge gas oxygenation, reduce energy consumption, improve the homogeneity of mixing, stirring and material transfer, promote overall fermentation yield and efficiency.
Brief description of the drawings
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only embodiment more of the present utility model, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the disclosed a kind of gamma-polyglutamic acid-float bed type structure of reactor schematic diagram of the utility model embodiment.
Numeral and the represented corresponding component title of letter in figure:
1. tank body 2. temperature control interlayer 3. thermal insulation layer 4. water outlets
5. head sealing cover 6. tensimeter 7. safety valve 8. constant pressure valves
9. step-down gear 10. motor 11. thermometer 12. main shafts
The reinforced volume pump 16.pH monitor of 13. Rotating Stainless Steel Cage 14. upflow tube 15.
17. circulation feed bin 18. recycle pump 19. gas distribution pipe 20. discharge ports
21. air valves 22. purify air pump 23. cold water outlet 24. hot water mouths
25. pneumatic panel 26. plane bacterium bed 27. ring-type bacterium beds
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is only the utility model part embodiment, instead of whole embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtaining under creative work prerequisite, all belong to the scope of the utility model protection.
According to Fig. 1, the utility model provides a kind of gamma-polyglutamic acid-float bed type reactor, comprises tank body 1, temperature control interlayer 2, thermal insulation layer 3, water outlet 4, head sealing cover 5, tensimeter 6, safety valve 7, constant pressure valve 8, step-down gear 9, motor 10, thermometer 11, main shaft 12, Rotating Stainless Steel Cage 13, upflow tube 14, reinforced volume pump 15, pH monitor 16, circulation feed bin 17, recycle pump 18, gas distribution pipe 19, discharge port 20, air valve 21, purifies air pump 22, cold water outlet 23, hot water mouth 24, pneumatic panel 25, plane bacterium bed 26 and ring-type bacterium bed 27.
Described tank body 1 is made up of right cylinder, cone and head sealing cover 5, and described right cylinder lower end and cone are welded and fixed, and described right cylinder upper end is provided with ring flange, and described ring flange is connected by bolt seal with described head sealing cover 5, described tank body 1 is provided with temperature control interlayer 2, and described temperature control interlayer 2 arranged outside have thermal insulation layer 3, described temperature control interlayer 2 tops are provided with water outlet 4, and the cone part of described temperature control interlayer 2 is provided with hot water mouth 24 and cold water outlet 23, on described head sealing cover 5, be provided with constant pressure valve 8 and thermometer 11, described constant pressure valve 8 bypasses are provided with safety valve 7 and tensimeter 6, described head sealing cover 5 central positions are provided with main shaft 12, and described main shaft 12 upper ends are connected with step-down gear 9, and described step-down gear 9 is connected with motor 10, and described main shaft 12 is turning axle, and the lower end of described main shaft 12 is connected with the sleeve sealing at pneumatic panel 25 centers, described tank body 1 cone bottom is provided with discharge port 20, described discharge port 20 pipeline bypasses are communicated with air valve 21 and purification air pump 22, the cone inside of described tank body 1 is provided with gas distribution pipe 19, described tank body 1 lower end and cone intersection are provided with pneumatic panel 25, described tank body 1 inside is provided with Rotating Stainless Steel Cage 13, described Rotating Stainless Steel Cage 13 central positions and main shaft 12 are welded and fixed, described Rotating Stainless Steel Cage 13 is provided with plane bacterium bed 26 and ring-type bacterium bed 27, described plane bacterium bed 26 forms by fibrous reticulum is curling with ring-type bacterium bed 27, described fibrous reticulum is fixed by Rotating Stainless Steel Cage 13, in the time that described main shaft 12 rotates, drive Rotating Stainless Steel Cage 13 together to rotate, described plane bacterium bed 26 and ring-type bacterium bed 27 are also rotated simultaneously in tank body 1, described plane bacterium bed 26 and ring-type bacterium bed 27 in kinestate, play stirring, mixing effect for fermentation materials, is being uniformly mixed under effect, and to fermented liquid, performance increases the effect of oxygen supply, transmitter substance, described tank body 1 top, side arranges upflow tube 14, described tank body 1 cone bottom is provided with cycle stock mouth, described upflow tube 14 is communicated with described circulation feed bin 17 tops, and described circulation feed bin 17 bottoms are communicated with recycle pump 18 by pipeline, and described recycle pump 18 is communicated with cycle stock mouth by pipeline, described circulation feed bin 17 tops are provided with pH monitor 16 and charging opening, and described charging opening and described reinforced volume pump 15 are by pipeline communication, and described reinforced volume pump 15 is communicated with (not marking) by pipeline with feeding chamber, described purification air pump 22 passes through pipeline communication with air-purification system (not marking).
Described plane bacterium bed 26 arranges 5 layers, and described ring-type bacterium bed 27 at least arranges 2 layers; The thickness of described plane bacterium bed 26 is 100mm, and the thickness of described ring-type bacterium bed 27 is 80mm.
Rotating Stainless Steel Cage 13 structures that described plane bacterium bed 26 arranges for circular flat, described fibrous reticulum is vertically wound in Rotating Stainless Steel Cage 13; Described ring-type bacterium bed 27 is Rotating Stainless Steel Cage 13 structures that annular arranges, and described fibrous reticulum is horizontally set in ring stainless steel cage 13; Described plane bacterium bed 26 is welded and fixed each other with the Rotating Stainless Steel Cage 13 of ring-type bacterium bed 27; The fibrous reticulum of described plane bacterium bed 26 and the 27 interior settings of ring-type bacterium bed, all perpendicular with the tangent line of sense of rotation, the fermentation liquid material body in therefore guaranteeing described fibrous reticulum and flowing fully mixes and stirring;
Described gas distribution pipe 19 is communicated with described discharge port 20, therefore purifying air of discharge port 20 bypasses can be sent into fermentor tank by purifying air pump 22, described gas distribution pipe 19 is at least provided with two, described gas distribution pipe 19 is uniformly distributed along described cone surface angle of inclination in tank body 1 cone, is provided with multiple uniform qi-emitting holes on the tube wall of described gas distribution pipe.
On described pneumatic panel 25, be provided with uniform air distribution hole.
Between described adjacent plane bacterium bed 26, distance is 200mm.
Distance between described adjacent ring-type bacterium bed 27 is 200mm.
In described tank body 1, dose gamma-polyglutamic acid-fermented liquid, in described circulation feed bin 17, store the fermented liquid overflowing, described fermentor tank Flow of Goods and Materials, mix, stir and drive fermented liquid by recycle pump 18, enter tank body 1 cone part from fermentor tank bottom, and then after uniform by pneumatic panel 25, enter described plane bacterium bed 26 and ring-type Jun Chuan 27th district, fermented liquid is under recycle pump 18 pressure-driven, successively by plane bacterium bed 26 and ring-type bacterium bed 27, active ingredient in fermented liquid, can be attached in the reticulin fiber of fiber, between adjacent plane bacterium bed 26 and ring-type bacterium bed 27, because bacterium bed is in rotating state, fermented feed liquid is subject to the rising motivating force effect of centrifugal force and recycle pump 18, make the fermented liquid of flowing through be able to fully stir and mix, along with the rising of fermented liquid and the increase of recycle pump 18 mass transfer amounts, the fermentation liquid measure of tank body 1 inside increases, liquid level raises, in the time that fermented liquid liquid level meets and exceeds upflow tube 14, fermented liquid can flow in circulation feed bin 17 by upflow tube 14, enter next round circulation mass transfer process.
Described air, after purifying by purification system, be sent in gas distribution pipe 19, through gas distribution pipe 19 gas distributions, be uniformly distributed gas, and fully contact with the fermented liquid in tank body 1 cone, recycle pump 18 transmit fermented liquid flow under, by pneumatic panel 25 further gas is mixed with fermented liquid and distribution; Described purifying air risen together and enters in plane bacterium bed 26 and ring-type bacterium bed 27 in company with fermented liquid, and by the multiple plane bacterium beds 26 and the ring-type bacterium bed that arrange on Rotating Stainless Steel Cage 13, for free live flora in the live flora in each bacterium bed and tank body brings oxygen, and then increase the exchange of oxygen and the transmission of fermented liquid exchange, described air is after each layer plane bacterium bed 26 and ring-type bacterium bed 27, rise to tank body top, and outwards discharge by constant pressure valve intermittent type, guarantee the pressure that tank interior is certain, therefore can promote the oxygen level in fermented liquid.
In described fermentor tank, the temperature of fermented liquid, carries out thermostatic control by hot water and the cold water of heat exchanger; Pressure in described tank body 1 and temperature data detect by tensimeter 6 and thermometer 11; During the fermentation, the culture base-material body that needs stream to add in fermentation system, quantitatively add by reinforced volume pump 15, pH value and total residual sugar amount in fermentation system are monitored by circulation feed bin 17, and regulate by the pH value to circulation feed bin 17, control the every fermentation parameter in whole fermentation system.
When after the fermented liquid fermentation ends in fermentor tank, by discharge port 20, fermented liquid is discharged to system, further carry out Separation & Purification, obtain gamma-polyglutamic acid-solution or sterling crystal; And active thalline in fermentation system can be deposited in fiber bacterium bed, dose the fermented liquid of next batch and can proceed gamma-polyglutamic acid-fermentation, thereby can realize multiple batches of continuous production.
By above-mentioned specific embodiment, the beneficial effects of the utility model are: immobilized thallus fermentation is combined with the fermentation of free type thalline, adopt thalline float bed type structure, use Rotating Stainless Steel Cage structure fibrous reticulum to be assemblied in the stainless copper cage of different structure, simultaneously, drive cage body to be rotated by motor, promote on the one hand thalline implantation, play on the other hand stirring, mixing effect, by Purge gas oxygenation, reduce energy consumption, improve the homogeneity of mixing, stirring and material transfer, promote overall fermentation yield and efficiency.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the utility model.To be apparent for those skilled in the art to the multiple amendment of these embodiment, General Principle as defined herein can, in the situation that not departing from spirit or scope of the present utility model, realize in other embodiments.Therefore, the utility model will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.