CN203181661U - Mycorrhiza research simulation device for leguminous and graminaceous crop intercropping cultivation - Google Patents
Mycorrhiza research simulation device for leguminous and graminaceous crop intercropping cultivation Download PDFInfo
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- CN203181661U CN203181661U CN 201320241386 CN201320241386U CN203181661U CN 203181661 U CN203181661 U CN 203181661U CN 201320241386 CN201320241386 CN 201320241386 CN 201320241386 U CN201320241386 U CN 201320241386U CN 203181661 U CN203181661 U CN 203181661U
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- 238000009342 intercropping Methods 0.000 title claims abstract description 19
- 238000004088 simulation Methods 0.000 title claims abstract description 12
- 239000004677 Nylon Substances 0.000 claims abstract description 20
- 229920001778 nylon Polymers 0.000 claims abstract description 20
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 23
- 239000004800 polyvinyl chloride Substances 0.000 claims description 23
- 239000000565 sealant Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 238000010521 absorption reaction Methods 0.000 abstract description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 8
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 8
- 239000011574 phosphorus Substances 0.000 abstract description 8
- 235000021374 legumes Nutrition 0.000 abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 239000000853 adhesive Substances 0.000 abstract description 3
- 230000001070 adhesive effect Effects 0.000 abstract description 3
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 abstract 1
- 229920002554 vinyl polymer Polymers 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 241000209504 Poaceae Species 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004178 biological nitrogen fixation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
本实用新型涉及一种豆科、禾本科间作栽培的菌根研究模拟装置,属于菌根技术领域;装置包括PVC圆管、卡槽、尼龙网、栽培室、圆形底板、小孔;装置呈圆桶型,采用PVC圆管制成,沿PVC圆管直径从底部向上至顶部2cm处设有纵向卡槽,将尼龙网固定在卡槽内将PVC圆管隔成两个栽培室,并用聚氯乙烯粘合剂密封,装置上端开口,底端采用圆形底板封闭,在两栽培室底部的中间位置分别开有的小孔。本实用新型用于研究宿主为禾本科作物的菌根结构,特别是菌丝对豆科植物种植土壤中氮、磷的吸收、利用,同时,排除了豆科和禾本科作物根系对养分的竞争吸收,本实用新型结构简单、造价低廉、实用性强。
The utility model relates to a mycorrhizal research simulation device for leguminous and gramineous intercropping cultivation, which belongs to the field of mycorrhizal technology; the device includes a PVC round pipe, a card slot, a nylon net, a cultivation room, a circular bottom plate, and a small hole; the device is Drum type, made of PVC round pipe, along the diameter of the PVC round pipe from the bottom up to the top 2cm, there is a longitudinal card slot, the nylon net is fixed in the card slot, the PVC round tube is divided into two cultivation rooms, and polychlorinated Sealed with vinyl adhesive, the upper end of the device is open, and the bottom end is closed with a circular bottom plate, and there are small holes in the middle of the bottom of the two cultivation chambers. The utility model is used to study the mycorrhizal structure of gramineous crops as the host, especially the absorption and utilization of nitrogen and phosphorus in legume planting soil by mycelia, and at the same time, it eliminates the competition of leguminous and gramineous crop roots for nutrients absorption, the utility model has the advantages of simple structure, low cost and strong practicability.
Description
技术领域 technical field
本实用新型涉及一种豆科、禾本科间作栽培的菌根研究模拟装置,属于菌根技术领域。 The utility model relates to a mycorrhizal research simulation device for the intercropping cultivation of legumes and grasses, which belongs to the technical field of mycorrhizae.
背景技术 Background technique
农作物间作是我国传统的农业种植模式之一,它能够充分利用光、热、水、土资源,提高体系中的农作物产量。随着资源、环境、粮食等全球性问题的严峻挑战,间作再次引起科学家们的广泛关注。其中豆科/禾本科间作由于是高矮杆植物的搭配,且豆科作物如大豆等能进行生物固氮,禾本科作物如玉米等还具有较强的菌根依耐性,因而这一间作所体现出在养分如磷素利用上优势更为明显。 Crop intercropping is one of the traditional agricultural planting modes in my country. It can make full use of light, heat, water, and soil resources to increase the crop yield in the system. With the severe challenges of global issues such as resources, environment, and food, intercropping has once again attracted widespread attention of scientists. Among them, leguminous/gramineous intercropping is a combination of tall and dwarf plants, and leguminous crops such as soybeans can carry out biological nitrogen fixation, and gramineous crops such as corn have strong mycorrhizal tolerance, so this intercropping reflects The advantages are more obvious in the utilization of nutrients such as phosphorus.
目前通过精细的盆栽模拟试验如采用不同根系分隔方式对豆科/禾本科间作条件下根系对养分如氮、磷竞争吸收利用的研究较多,李淑敏等(中国生态农业学报,2005,13(3):136-139)采用简易塑料盆把菌根因素结合到豆科/禾本科作物间作体系中对根系磷吸收竞争进行了研究,不过所制作的塑料盆间作装置可能会存在着粘合尼龙网难度较大和密封效果不太好的问题,且在作物种植培养过程中根系的生长还可能受上宽下窄的塑料盆形状影响的问题。 At present, there are many studies on the competitive absorption and utilization of nutrients such as nitrogen and phosphorus by roots under the condition of leguminous/gramineous intercropping through fine pot simulation experiments such as using different root separation methods. Li Shumin et al. (Chinese Journal of Ecological Agriculture, 2005, 13 (3 ): 136-139) Using simple plastic pots to combine mycorrhizal factors into the legume/grass intercropping system to study the competition for root phosphorus uptake, but the plastic pot intercropping device may have bonded nylon mesh It is difficult and the sealing effect is not very good, and the growth of the root system may also be affected by the shape of the plastic pot with a wide top and a narrow bottom during crop planting and cultivation.
因此为了更好区分菌根菌丝在豆科/禾本科间作体系中与根系对土壤氮、磷等养分活化利用的作用及贡献,亟待对上述装置所采用的材料与设计进行改进和创新。 Therefore, in order to better distinguish the role and contribution of mycorrhizal mycelium and root system to the activation and utilization of soil nitrogen, phosphorus and other nutrients in the leguminous/graminaceous intercropping system, it is urgent to improve and innovate the materials and designs used in the above-mentioned devices.
实用新型内容 Utility model content
针对上述问题,本实用新型提供一种豆科、禾本科间作栽培的菌根研究模拟装置,用于研究宿主为禾本科作物的菌根结构,如菌丝对豆科植物种植土壤中养分如氮、磷的吸收利用,且排除了豆科和禾本科作物根系对养分的竞争吸收,同时,本实用新型结构简单、造价低廉、实用性强。 In view of the above problems, the utility model provides a mycorrhizal research simulation device for leguminous and gramineous intercropping cultivation, which is used to study the mycorrhizal structure of gramineous crops as the host, such as mycelium's effect on nutrients such as nitrogen in the leguminous planting soil. , the absorption and utilization of phosphorus, and eliminate the competitive absorption of nutrients by the roots of leguminous and gramineous crops. At the same time, the utility model has the advantages of simple structure, low cost and strong practicability.
为了实现实用新型目的,本实用新型采用的技术方案:一种豆科、禾本科间作栽培的菌根研究模拟装置包括PVC圆管1、卡槽2、尼龙网3、栽培室4、圆形底板5、小孔6;装置呈圆桶型,采用PVC圆管1制成,沿PVC圆管直径从底部向上至顶部2 cm处设有纵向卡槽2,将尼龙网3固定在卡槽内将PVC圆管隔成两个栽培室4,并用聚氯乙烯粘合剂密封,装置上端开口,底端采用圆形底板5封闭,在两栽培室底部的中间位置分别开有的小孔6。 In order to realize the purpose of the utility model, the technical solution adopted in the utility model: a mycorrhizal research simulation device for leguminous and gramineous intercropping cultivation includes a PVC round pipe 1, a slot 2, a nylon net 3, a cultivation room 4, and a circular bottom plate 5. Small hole 6; the device is barrel-shaped, made of PVC round pipe 1, and a longitudinal slot 2 is provided along the diameter of the PVC round pipe from the bottom up to the top 2 cm, and the nylon mesh 3 is fixed in the slot The PVC round pipe is divided into two cultivation chambers 4, and is sealed with a polyvinyl chloride adhesive. The upper end of the device is open, and the bottom end is closed with a circular bottom plate 5, and there are small holes 6 in the middle of the bottom of the two cultivation chambers.
所述的PVC圆管1的直径为16~20 cm,高度为23~25 cm。 The diameter of described PVC pipe 1 is 16~20 cm, and height is 23~25 cm.
所述的PVC圆管1分为四层,从上到下高度依次为第一层3厘米,第二层8 cm,第三层8cm,第四层4~6 cm。 The PVC round pipe 1 is divided into four layers, the height from top to bottom is 3 cm for the first layer, 8 cm for the second layer, 8 cm for the third layer, and 4 to 6 cm for the fourth layer.
所述的尼龙网3采用30 μm孔径的尼龙网。 Described nylon mesh 3 adopts the nylon mesh of 30 μm aperture.
所述的小孔6直径为0.5~1.0 cm。 The diameter of the small hole 6 is 0.5-1.0 cm.
所述的PVC圆管1、卡槽2、尼龙网3、圆形底板5均为市售的普通元件。 The PVC round pipe 1, the card slot 2, the nylon net 3, and the circular bottom plate 5 are all commercially available common components.
本实用新型的有益效果:本实用新型用于研究宿主为禾本科作物的菌根结构,特别是菌丝对豆科植物种植土壤中氮、磷的吸收、利用,同时,排除了豆科和禾本科作物根系对养分的竞争吸收,本实用新型结构简单、造价低廉、实用性强。 Beneficial effects of the utility model: the utility model is used to study the mycorrhizal structure of the host of gramineous crops, especially the absorption and utilization of nitrogen and phosphorus in the planting soil of leguminous plants by mycelia. The root system of undergraduate crops competes to absorb nutrients, and the utility model has the advantages of simple structure, low cost and strong practicability.
附图说明 Description of drawings
图1 为本实用新型的结构示意图; Fig. 1 is the structural representation of the utility model;
图中:1-PVC圆管、2-卡槽、3-尼龙网、4-栽培室、5-圆形底板、6-小孔。 In the figure: 1-PVC round pipe, 2-card slot, 3-nylon net, 4-cultivation room, 5-round bottom plate, 6-small hole.
具体实施方式 Detailed ways
下面结合附图和实施例对本实用新型作进一步说明,以方便技术人员理解。 The utility model will be further described below in conjunction with the accompanying drawings and embodiments, so as to facilitate the technical personnel's understanding.
如图1所示:一种豆科、禾本科间作栽培的菌根研究模拟装置包括PVC圆管1、卡槽2、尼龙网3、栽培室4、圆形底板5、小孔6;装置呈圆桶型,采用PVC圆管1制成,这样易于取材,同时降低成本,为了便于固定而不采用上下全切开的方式,而是沿PVC圆管直径从底部向上至顶部2 cm处设有纵向卡槽2,将尼龙网3固定在卡槽内将PVC圆管隔成两个栽培室4,并用聚氯乙烯粘合剂密封,装置上端开口,底端采用圆形底板5封闭,在两栽培室底部的中间位置分别开有的小孔6。 As shown in Figure 1: a mycorrhizal research simulation device for leguminous and gramineous intercropping cultivation comprises PVC round pipe 1, draw-in groove 2, nylon net 3, cultivation chamber 4, circular bottom plate 5, small hole 6; The barrel type is made of PVC round pipe 1, which is easy to obtain materials and reduces costs. In order to facilitate fixing, it does not use the method of cutting up and down, but along the diameter of the PVC round pipe from the bottom up to the top 2 cm. Longitudinal draw-in slot 2, nylon net 3 is fixed in the draw-in slot and the PVC round pipe is divided into two cultivation chambers 4, and seal with polyvinyl chloride adhesive, the upper end of the device is open, and the bottom end is closed with a circular bottom plate 5. There are small holes 6 in the middle of the bottom of the cultivation chamber.
所述的PVC圆管1的直径为16~20 cm,高度为23~25 cm。 The diameter of described PVC pipe 1 is 16~20 cm, and height is 23~25 cm.
所述的PVC圆管1分为四层,从上到下高度依次为第一层3厘米,第二层8 cm,第三层8cm,第四层4~6 cm高;第一层距顶部3 cm处、高于尼龙网顶部1 cm,这样可避免土壤因浇水溅起从一栽培室室进入另一栽培室室,其中土壤盛装在第二层、第三层、第四层中,两个栽培室4均盛装灭菌土壤,土壤过2mm筛后在120℃下蒸汽灭菌2 h,晾干。 The PVC round pipe 1 is divided into four layers, the height from top to bottom is 3 cm for the first layer, 8 cm for the second layer, 8 cm for the third layer, and 4 to 6 cm for the fourth layer; 3 cm, 1 cm higher than the top of the nylon net, so that the soil can be prevented from entering another cultivation room from one cultivation room due to water splashing, wherein the soil is contained in the second, third and fourth layers, The two cultivation chambers 4 are filled with sterilized soil, and the soil is steam sterilized at 120°C for 2 hours after passing through a 2mm sieve, and then dried in the air.
所述的尼龙网3采用30 μm孔径的尼龙网,这样菌根真菌的菌丝可以穿过,而根系不能穿过而被限制在各自的生长空间里。 Described nylon mesh 3 adopts the nylon mesh of 30 μ m apertures, and the mycelium of mycorrhizal fungus can pass through like this, and root system can not pass through and is limited in the growth space respectively.
所述的小孔6直径为0.5~1.0 cm,以便于透水透气。 The diameter of the small hole 6 is 0.5 ~ 1.0 cm, so as to facilitate water and air permeability.
本实用新型在使用时,两个栽培室4中一个栽培室种植接种有菌根真菌(中层土壤与约 30~50 g菌根菌剂混匀后加入)的禾本科植物,另一栽培室种植豆科植物。豆科和禾本科种子经双氧水 消毒、催芽、播种覆薄层土后,上面再撒一薄层灭过菌的石英沙以减少水分蒸发。出苗后,豆科植物和禾本科植物各留3~4棵和2~3棵,通过称重法和浇灌蒸馏水保持适宜的土壤含水量,并在一定的温度、湿度及光照条件下的温室或大棚或人工气候室里培养2 ~3月即可取样或收获,由于本实用新型设置了尼龙网,使根系限制在两个栽培室4生长,而菌根菌丝可以穿过尼龙网到边室土壤中吸收养分,达到将根系吸收区隔开的目的。 When the utility model is in use, one of the two cultivation chambers 4 is planted with gramineous plants inoculated with mycorrhizal fungi (the middle layer soil is mixed with about 30-50 g of mycorrhizal fungal agents), and the other cultivation chamber is planted with legumes. After the seeds of legumes and grasses are sterilized with hydrogen peroxide, germinated, and sown with a thin layer of soil, a thin layer of sterilized quartz sand is sprinkled on top to reduce water evaporation. After emergence, keep 3~4 plants and 2~3 plants of leguminous plants and gramineous plants respectively, maintain suitable soil moisture content by weighing method and watering distilled water, and store them in a greenhouse or under certain temperature, humidity and light conditions. Sampling or harvesting can be done after 2 to 3 months of cultivation in greenhouses or artificial climate chambers. Because the utility model is equipped with nylon nets, the root system is limited to grow in the two cultivation chambers 4, and mycorrhizal hyphae can pass through the nylon nets to the side chambers. Absorb nutrients in the soil to achieve the purpose of separating the root absorption zone.
本实用新型用于研究宿主为禾本科作物的菌根结构,特别是菌丝对豆科植物种植土壤中氮、磷的吸收、利用,同时,排除了豆科和禾本科作物根系对养分的竞争吸收,本实用新型结构简单、造价低廉、实用性强。 The utility model is used to study the mycorrhizal structure of gramineous crops as the host, especially the absorption and utilization of nitrogen and phosphorus in legume planting soil by mycelia, and at the same time, it eliminates the competition of leguminous and gramineous crop roots for nutrients absorption, the utility model has the advantages of simple structure, low cost and strong practicability.
本实用新型通过附图进行说明的,在不脱离本实用新型范围的情况下,还可以对本实用新型专利进行各种变换及等同代替,因此,本实用新型专利不局限于所公开的具体实施过程,而应当包括落入本实用新型专利权利要求范围内的全部实施方案。 The utility model is illustrated by the accompanying drawings. Without departing from the scope of the utility model, various transformations and equivalent substitutions can be made to the utility model patent. Therefore, the utility model patent is not limited to the disclosed specific implementation process. , but should include all the implementations that fall within the scope of the utility model patent claims.
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Cited By (2)
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CN104186198A (en) * | 2014-07-02 | 2014-12-10 | 贵州大学 | Device for transferring nutrients among plants |
CN104322248A (en) * | 2014-10-09 | 2015-02-04 | 吉林省农业科学院 | Layered positioning study method for plant root system growth and layered positioning device |
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CN104186198A (en) * | 2014-07-02 | 2014-12-10 | 贵州大学 | Device for transferring nutrients among plants |
CN104322248A (en) * | 2014-10-09 | 2015-02-04 | 吉林省农业科学院 | Layered positioning study method for plant root system growth and layered positioning device |
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