CN202305534U - Western blotting antibody incubation box - Google Patents
Western blotting antibody incubation box Download PDFInfo
- Publication number
- CN202305534U CN202305534U CN2011204488459U CN201120448845U CN202305534U CN 202305534 U CN202305534 U CN 202305534U CN 2011204488459 U CN2011204488459 U CN 2011204488459U CN 201120448845 U CN201120448845 U CN 201120448845U CN 202305534 U CN202305534 U CN 202305534U
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- box
- western blotting
- tooth
- antibody incubation
- antibody
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Abstract
The utility model provides a western blotting antibody incubation box; teeth which are extended into a box body are arranged on the inner wall of the box body; and teeth notches are formed between the teeth. The western blotting antibody incubation box also comprises division plates; and the widths of the division plates are matched with the widths of the teeth notches. The western blotting antibody incubation box is simple and easy to use, effectively increases the volume utilization ratio of an antibody solution in an antibody incubation step of a western blotting experiment and reduces the antibody solution in the premise of ensuring experiment results, thereby saving the antibody. Simultaneously, the floatation of a nitrocellulose membrane in the incubation box is avoided, and the best experiment result is ensured when the few antibody solution is ensured.
Description
Technical field
The utility model relates to a kind of case type container that nitrocellulose filter and antibody-solutions are hatched altogether of in Western blotting (Western Blot) experiment, being used for, and is specifically related to a kind of simple, easy-to-use Western blotting antibody incubation box.
Background technology
Immunoblot experiment is a common technology in the current biological scientific research.In experiment, protein is through being transferred behind the polyacrylamide gel electrophoresis and being fixed on the nitrocellulose filter.After nitrocellulose filter and specific antibody-solutions hatched altogether, through rinsing, steps such as colour developing can be observed combining of specific antigen composition in antibody and the albumen.Wherein, to hatch altogether be one of committed step of experiment for nitrocellulose filter and antibody-solutions.The antibody that in this step, uses is precious and expensive expendable reagent, needs to save to use as far as possible.On the other hand, antibody-solutions must reach finite concentration and just can make experiment reach promising result.Therefore, one of important method of saving antibody is the use volume that reduces antibody-solutions, gets final product among making that nitrocellulose filter is just complete and being immersed in antibody-solutions.
At present, the antibody incubation step is many carries out in sealed plastic bag or case type container.Use the advantage of sealed plastic bag to be: the big I of bag cuts arbitrarily according to the size of nitrocellulose filter, will hatch area and reduce to bottom line (only membrane density is long-pending big slightly).But be difficult to when using sealed plastic bag guarantee do not have bubble to be enclosed in the bag.Because of the antibody-solutions viscosity is bigger, surface tension is higher, even on shaking table, do not stop to shake, the bubble of being enclosed in the bag still might adhere to film surface ad-hoc location, and causes this position and antibody response abundant inadequately, influences test mass.When in case type container, hatching, antibody-solutions flows better, is easy to the homogeneity that guarantees that the film surface contacts with antibody everywhere.But under the rocking of shaking table, nitrocellulose filter is easily floating, make part film surface break away from antibody-solutions and expose with air in.In practical operation, often have to strengthen the antibody-solutions volume and solve this problem, and bigger liquid solution volume means more antibody consumption to obtain better liquid fluidity.In addition, because of each batch number of test specimens differs, the differing in size of film.In reality, be difficult to prepare a plurality of boxes that differ in size to adapt to the size of film.Usually all be to use a bigger box.Like this, hour caused the waste of antibody again when the area of film.
The utility model content
To the deficiency that exists in the prior art, the utility model provides a kind of simple, easy-to-use Western blotting antibody incubation box.
The Western blotting antibody incubation box that the utility model provides, the inwall of box body is provided with the tooth that in box body, extends.Said tooth is perpendicular to the inwall at tooth place.The inwall at perhaps said tooth and tooth place forms the inclination angle, and said inclination angle is more than or equal to 45 degree and less than 90 degree.
Described Western blotting antibody incubation box, the height of said tooth is more than or equal to 1/2 dark height of box.Form mark of mouth between said tooth and the tooth, the width of said mark of mouth is identical or different.
Described Western blotting antibody incubation box also comprises demarcation strip, and the width of said demarcation strip and the width of said mark of mouth are suitable.The height of said demarcation strip is more than or equal to 1/2 dark height of box, and the length of said demarcation strip is with suitable with the width of box.The periphery of said demarcation strip studs with sealing strip.Sealing strip is preferably the silica gel sealing bar.
Preferably, described Western blotting antibody incubation box all is provided with said tooth on two of box body relative inwalls, said tooth perpendicular to the inwall at tooth place and with box height such as grade deeply, said demarcation strip and box be the height such as grade deeply, and the length of demarcation strip is identical with the width of box.
The Western blotting antibody incubation box that the utility model provides is simple, easy-to-use; Improve the volume utilization of antibody-solutions in the immunoblot experiment antibody incubation step effectively; Under the prerequisite that guarantees experiment effect, reduce the use of antibody-solutions, thus the use of practicing thrift antibody.Avoided nitrocellulose filter floating in hatching box simultaneously, the best experiment effect when having guaranteed to use a small amount of antibody-solutions.
For let the above-mentioned of the utility model and other purpose, feature and advantage can be more obviously understandable, hereinafter is special lifts preferred embodiment, and conjunction with figs., elaborates as follows.
Description of drawings
Fig. 1 is the front elevational schematic of a preferred embodiment of the Western blotting antibody incubation box of the utility model;
Fig. 2 is the front elevational schematic of another preferred embodiment of the Western blotting antibody incubation box of the utility model;
Fig. 3 is the front elevational schematic of another preferred embodiment of the Western blotting antibody incubation box of the utility model;
Fig. 4 is the front elevational schematic of another preferred embodiment of the Western blotting antibody incubation box of the utility model.
Embodiment
With reference to Fig. 1, it is the synoptic diagram of the Western blotting antibody incubation box that provides of the utility model, and this this Western blotting antibody incubation box can be by polypropylene or known other the suitable made of those skilled in the art.Wherein box body 1 can be illustrated rectangular parallelepiped, can also be other suitable shape.As shown in Figure 1, the inwall of box body 1 is provided with a plurality of teeth 2 that in box body, extend, and can on an inwall, tooth 2 be set, and also can tooth 2 be set on a plurality of inwalls, like Fig. 3, shown in Figure 4.For example, can on two of box body relative inwalls, tooth 2 be set all.Through the setting of such multiple choices, can satisfy the multiple needs of different tests sample, thereby be suitable for the nitrocellulose filter of different sizes, saved experimentation cost, promoted test efficiency.
Like Fig. 1, shown in Figure 2, these teeth 2 can be perpendicular to the inwall at tooth place or the inwall formation inclination angle that belongs to tooth 2.According to the operating conditions of reality, the inclination angle is changeable, to be advisable more than or equal to 45 degree and less than 90 degree.
For the different situations of the various test specimens that adapt to practical operation, the height of tooth 2 is arranged to more than or equal to 1/2 dark height of box, for example can be arranged to with box height such as grade deeply, be advisable so that nitrocellulose filter is fully soaked in principle.
To shown in Figure 4, Western blotting antibody incubation box also comprises demarcation strip 3 like Fig. 1, and the width of the width of demarcation strip 3 and above-mentioned mark of mouth is suitable.Demarcation strip 3 can be a plurality of, thereby can be divided into a plurality of operable zones with hatching box, to adapt to the nitrocellulose filter of different sizes, makes full use of the space, saves antibody.
The height of demarcation strip can be preferably and box height such as grade deeply more than or equal to 1/2 dark height of box; The length of demarcation strip is with suitable with the width of box.The periphery of these demarcation strips studs with sealing strip, and preferably, sealing strip is the silica gel sealing bar.
When using Western blotting antibody incubation box; Elder generation inserts nitrocellulose filter at the bottom of the box; Select one or more demarcation strips according to the nitrocellulose filter of being put into then, the demarcation strip of selecting is put into suitable mark of mouth, the base of demarcation strip is pressed the limit of nitrocellulose filter at the bottom of box.By the silastic sealing strip of demarcation strip periphery, demarcation strip can form with the nitrocellulose filter area approximation less than the space of box floorage with the space that the inwall of hatching box forms.In this space, add antibody-solutions then.
For example, when selecting two demarcation strips, insert nitrocellulose filter at the bottom of the box after, two demarcation strips are inserted relative mark of mouth on the box inwall to be slightly smaller than the wide spacing distance of film, the base of demarcation strip is pressed the relative both sides of nitrocellulose filter at the bottom of box.Silastic sealing strip by the demarcation strip periphery can form with the nitrocellulose filter area approximation less than the space of box floorage between two demarcation strips.The antibody-solutions that adds in this space can have higher contacting efficiency with nitrocellulose filter.Simultaneously, nitrocellulose filter is pressed at the bottom of box and can't wave by two demarcation strips, only needs more a spot of antibody-solutions can guarantee the submergence of film.
When carrying out antibody incubation, the actual area of hatching can be adjusted to the size near membrane area.Simultaneously, nitrocellulose filter is pushed down with being flattened, pastes to obey at the bottom of box, can't hike up, guarantee a small amount of and the antibody-solutions that flows and film surface everywhere homogeneous contact, reach best experiment effect.
Though the utility model discloses as above with preferred embodiment; Right its is not in order to limit the utility model; Any person of ordinary skill in the field; In spirit that does not break away from the utility model and scope, when can doing a little change and improvement, so the protection domain of the utility model is as the criterion when looking the claim person of defining.
Claims (10)
1. a Western blotting antibody incubation box is characterized in that the inwall of box body is provided with the tooth that in box body, extends.
2. Western blotting antibody incubation box according to claim 1 is characterized in that, said tooth is perpendicular to the inwall at tooth place.
3. Western blotting antibody incubation box according to claim 1 is characterized in that, the inwall at said tooth and tooth place forms the inclination angle, and said inclination angle is more than or equal to 45 degree and less than 90 degree.
4. Western blotting antibody incubation box according to claim 1 is characterized in that, the height of said tooth is more than or equal to 1/2 dark height of box.
5. Western blotting antibody incubation box according to claim 1 is characterized in that form mark of mouth between tooth and the tooth, the width of said mark of mouth is identical or different.
6. according to each described Western blotting antibody incubation box of claim 1 to 5, it is characterized in that also comprise demarcation strip, the width of said demarcation strip and the width of said mark of mouth are suitable.
7. Western blotting antibody incubation box according to claim 6 is characterized in that, the height of said demarcation strip is more than or equal to 1/2 dark height of box, and the length of said demarcation strip is with suitable with the width of box.
8. Western blotting antibody incubation box according to claim 7 is characterized in that the periphery of said demarcation strip studs with sealing strip.
9. Western blotting antibody incubation box according to claim 8 is characterized in that said sealing strip is the silica gel sealing bar.
10. Western blotting antibody incubation box according to claim 9; It is characterized in that, on two of box body relative inwalls, said tooth is set all, said tooth perpendicular to the inwall at tooth place and with box height such as grade deeply; Said demarcation strip and box be the height such as grade deeply, and the length of demarcation strip is identical with the width of box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204488459U CN202305534U (en) | 2011-11-14 | 2011-11-14 | Western blotting antibody incubation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011204488459U CN202305534U (en) | 2011-11-14 | 2011-11-14 | Western blotting antibody incubation box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202305534U true CN202305534U (en) | 2012-07-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011204488459U Expired - Lifetime CN202305534U (en) | 2011-11-14 | 2011-11-14 | Western blotting antibody incubation box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202305534U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808941A (en) * | 2014-01-28 | 2014-05-21 | 河南科技大学第一附属医院 | Multifunctional protein reaction experiment device |
| CN103808942A (en) * | 2014-01-28 | 2014-05-21 | 河南科技大学第一附属医院 | Protein reaction box |
| CN105628916A (en) * | 2014-11-26 | 2016-06-01 | 欧蒙医学诊断技术有限公司 | Cultivation tray |
| CN111896746A (en) * | 2020-07-31 | 2020-11-06 | 复旦大学附属中山医院 | Western blot multi-antibody whole-membrane automatic incubation box |
-
2011
- 2011-11-14 CN CN2011204488459U patent/CN202305534U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103808941A (en) * | 2014-01-28 | 2014-05-21 | 河南科技大学第一附属医院 | Multifunctional protein reaction experiment device |
| CN103808942A (en) * | 2014-01-28 | 2014-05-21 | 河南科技大学第一附属医院 | Protein reaction box |
| CN103808941B (en) * | 2014-01-28 | 2015-08-26 | 河南科技大学第一附属医院 | A kind of multifunctional protein reaction experiment device |
| CN103808942B (en) * | 2014-01-28 | 2015-08-26 | 河南科技大学第一附属医院 | A kind of albumino reaction box |
| CN105628916A (en) * | 2014-11-26 | 2016-06-01 | 欧蒙医学诊断技术有限公司 | Cultivation tray |
| CN105628916B (en) * | 2014-11-26 | 2019-05-31 | 欧蒙医学诊断技术有限公司 | Culture tray |
| CN111896746A (en) * | 2020-07-31 | 2020-11-06 | 复旦大学附属中山医院 | Western blot multi-antibody whole-membrane automatic incubation box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180502 Address after: 225300 4 floor, building TQB, No. 1, Yao Cheng Avenue, Taizhou, Jiangsu. Patentee after: Jiangsu Kangwei Shiji Biological Science & Technology Co., Ltd. Address before: 100084 602A 1, building 1, Shang Di Road, Haidian District, Beijing. Patentee before: Beijing CoWin Bioscience Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 225300 4 floor, building TQB, No. 1, Yao Cheng Avenue, Taizhou, Jiangsu. Patentee after: Jiangsu Kangwei century Biotechnology Co.,Ltd. Address before: 225300 4 floor, building TQB, No. 1, Yao Cheng Avenue, Taizhou, Jiangsu. Patentee before: CoWin Biosciences Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120704 |
|
| CX01 | Expiry of patent term |