CN102707044B - Vertical western blot antibody incubation box - Google Patents
Vertical western blot antibody incubation box Download PDFInfo
- Publication number
- CN102707044B CN102707044B CN201210175168.7A CN201210175168A CN102707044B CN 102707044 B CN102707044 B CN 102707044B CN 201210175168 A CN201210175168 A CN 201210175168A CN 102707044 B CN102707044 B CN 102707044B
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- incubation
- box
- box body
- western blot
- vertical
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Abstract
The invention discloses a vertical western blot antibody incubation box, which comprises a box body (1) and a box cover, wherein a plurality of incubation grooves (3) are formed in the box body (1); the transverse section of each incubation groove (3) is hexagonal; the incubation grooves (3) transversely extend; communication holes (4) are formed in connected side faces of the incubation grooves (3); and a clamping slot (2) which is fastened with the box cover is formed on the upper outer surface of the box body (1). The invention has the advantages that 1) according to the vertical antibody incubation box, the size of membrane incubation antibodies is greatly reduced, the antibodies are saved, and cost is reduced; 2) by design of the hexagonal grooves, membranes can be prevented from being adhered to the box body, and incomplete membrane incubation is avoided; and 3) by height design of the communication holes of the incubation box, the quantity of a plurality of membranes which are simultaneously incubated can be controlled, efficiency is improved, and the antibodies are saved.
Description
Technical field
The present invention relates to the box of hatching that biological technical field uses, specifically refer to a kind of vertical Western blot antibody incubation box.
Background technology
In molecular biology experiment, western blotting technique is one of the most frequently used technology, for detection of the content of specific protein in sample.Protein electrophoresis and transferring film technology are very ripe, and all specifications comparatively of specification of equipment on market, and the size of film is more consistent.When protein delivery is to after on nitrocellulose membrane or nylon membrane, need further hatch with antibody, the time of hatching and the effect of hatching, owing to there is no the form of specification, have a variety of forms in actual mechanical process, are generally with hybridization bag or hatch box and hatch.Hybridization bag in use easily produces bubble, causes antibody incubation incomplete, hatches the antibody amount that box uses larger, easily causes waste, and film is easily attached to and hatches box bottom sometimes, likely causes and hatches not exclusively.Finally cause result repeatability bad, even produce false negative, affect the analysis of result.
Summary of the invention
In order to overcome above-mentioned defect, the invention provides a kind of vertical Western blot antibody incubation box, this device is in saving antibody incubation liquid, make the film can be equably and Incubating Solution effect, hexagonal incubation slot design can prevent that film is attached on device, the incubation slot of hatching in addition in box is connected by intercommunicating pore, and by the height control liquid level of intercommunicating pore, can hatch identical antibody, the parallel laboratory test of different films simultaneously.Hatch box and be furnished with corresponding lid, ensure to hatch the vaporization loss of liquid in process.
Technical scheme of the present invention is achieved in that and comprises box body (1) and lid, and in box body (1), establish some incubation slots (3), the xsect of described incubation slot (3) is hexagon, and described incubation slot (3) horizontal expansion in box body is placed.The side connecting between described incubation slot (3) has intercommunicating pore (4).The outer surface of upper of described box body (1) is provided with screens (2) and fastens with lid.
The invention has the beneficial effects as follows: vertical antibody incubation box 1), greatly reduce film and hatched the volume of antibody, save antibody, reduce costs; 2) design of hexagon groove can prevent that film is attached on box body, avoids film to hatch not exclusively; 3) hatch box by the height design of intercommunicating pore, can control the quantity of simultaneously hatching multiple films, raise the efficiency, save antibody.
Brief description of the drawings
Fig. 1 is the front view of box body of the present invention.
Fig. 2 is the side view of box body of the present invention.
Fig. 3 is the cut-open view of Figure 1A-A direction.
Fig. 4 is the cut-open view of Figure 1B-B direction.
Fig. 5 is the stereographic map of box body of the present invention.
Fig. 6 is the stereographic map of lid of the present invention.
In figure: 1. box body; 2. screens; 3. incubation slot; 4. intercommunicating pore.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further explained to explanation.
As shown in Fig. 1-6, vertical Western blot antibody incubation box, comprises box body 1 and lid, and in box body 1, establishing some xsects is hexagonal incubation slot 3, and incubation slot 3 horizontal expansion in box body is placed.Described incubation slot 3 also can be designed as the hexagon that xsect is prolate, and the distance between adjacent incubation slot 3 fillets is as far as possible little, can greatly reduce film like this and hatch the volume of antibody, saves antibody.In addition, hexagonal incubation slot 3 designs can prevent that film is attached on device, and the side connecting between described incubation slot 3 has intercommunicating pore 4, and by the height control liquid level of intercommunicating pore 4, can hatch identical antibody, the parallel laboratory test of different films simultaneously.The outer surface of upper of described box body 1 is provided with screens 2 and fastens with lid, makes whole device good airproof performance, reduces the vaporization loss of hatching liquid in process.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; any be familiar with those skilled in the art the present invention disclose technical scope in; be equal to replacement or changed according to technical scheme of the present invention and inventive concept thereof, within all should being encompassed in protection scope of the present invention.
Claims (3)
1. vertical Western blot antibody incubation box, comprises box body (1) and lid, establishes some incubation slots (3) in box body (1), it is characterized in that, the xsect of described incubation slot (3) is hexagon, and described incubation slot (3) horizontal expansion in box body is placed.
2. vertical Western blot antibody incubation box according to claim 1, is characterized in that, the side connecting between described incubation slot (3) has intercommunicating pore (4).
3. vertical Western blot antibody incubation box according to claim 1, is characterized in that, the outer surface of upper of described box body (1) is provided with screens (2) and fastens with lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210175168.7A CN102707044B (en) | 2012-05-31 | 2012-05-31 | Vertical western blot antibody incubation box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210175168.7A CN102707044B (en) | 2012-05-31 | 2012-05-31 | Vertical western blot antibody incubation box |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102707044A CN102707044A (en) | 2012-10-03 |
| CN102707044B true CN102707044B (en) | 2014-08-27 |
Family
ID=46899981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210175168.7A Expired - Fee Related CN102707044B (en) | 2012-05-31 | 2012-05-31 | Vertical western blot antibody incubation box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102707044B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111254044A (en) * | 2018-12-01 | 2020-06-09 | 艾康生物技术(杭州)有限公司 | Reaction cup, molecular hybridization instrument, application of molecular hybridization instrument and detection method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2156511Y (en) * | 1993-05-03 | 1994-02-16 | 戴金 | Bar shape solid phase material and reaction concave trough for enzyme jmmunity testing |
| CN2577283Y (en) * | 2002-11-08 | 2003-10-01 | 穆海东 | Protein chip |
| CN2777542Y (en) * | 2004-11-26 | 2006-05-03 | 珠海市永如生物技术发展有限公司 | Combined covex type many person portion protein chip |
| CN101479609B (en) * | 2006-06-30 | 2012-09-05 | 松下电器产业株式会社 | Panel for analysis and analyzer using the same |
| JP4859769B2 (en) * | 2006-06-30 | 2012-01-25 | パナソニック株式会社 | Analysis panel and analyzer using the same |
| CN201583542U (en) * | 2009-09-08 | 2010-09-15 | 刘毅 | Protein electrophoresis antibody incubation box |
| CN201796033U (en) * | 2010-02-02 | 2011-04-13 | 中国农业科学院生物技术研究所 | Gold-labeled kit capable of quickly detecting glyphosate-resistant G2-EPSPS protein |
-
2012
- 2012-05-31 CN CN201210175168.7A patent/CN102707044B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102707044A (en) | 2012-10-03 |
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Legal Events
| Date | Code | Title | Description |
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| C06 | Publication | ||
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| ASS | Succession or assignment of patent right |
Owner name: DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACAD Effective date: 20140714 Owner name: LIAONING INTERNATIONAL TRAVEL HEALTH CARE CENTER Free format text: FORMER OWNER: LI YUNFENG Effective date: 20140714 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Xiao Inventor after: Li Yunfeng Inventor after: Zhang Min Inventor after: Sun Shi Inventor after: Cang Yuting Inventor after: Ge Teng Inventor before: Li Yunfeng |
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| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LI YUNFENG TO: LI XIAO LI YUNFENG ZHANG MIN SUN SHI ZANG YUTING GE TENG |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20140714 Address after: 116001, No. 60, Changjiang East Road, Zhongshan District, Liaoning, Dalian Applicant after: Liaoning international travel health care center Applicant after: Dalian Institute of Chemical Physics, Chinese Academy of Sciences Applicant after: Guangdong international travel health care center Address before: 116001 No. 39 Renmin Road, Zhongshan District, Liaoning, Dalian Applicant before: Li Yunfeng |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140827 Termination date: 20160531 |