CN201583542U

CN201583542U - Protein electrophoresis antibody incubation box

Info

Publication number: CN201583542U
Application number: CN2009201756307U
Authority: CN
Inventors: 刘毅
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-09-08
Filing date: 2009-09-08
Publication date: 2010-09-15
Anticipated expiration: 2019-09-08

Abstract

The utility model discloses an incubation box for incubating antibodies, used in molecular biology experiment-protein electrophoresis. The incubation box is designed into four specifications, according to universal electrophoresis in biology experiment, protein containing a membrane and obtained by membrane transferring, as well as different number of loaded bands and the property of target protein in the possible concrete experiment. Slots are inserted in the incubation box to realize the simultaneous treatment of at most four membranes containing protein with the same antibody condition. The inserters of the concave slots are hollow, so that antibody incubation solution can circulate among all compartments of the incubation box through the inserters.

Description

Protein electrophoresis antibody incubation box

Technical field

The utility model relates in the molecular biological protein electrophoresis method, and the film behind the protein on changeing is carried out the operation of antibody incubation.Fundamental purpose is in order to realize according to containing the size of protein film in the test, and the required property of protein difference of making, and is equipped with the antibody incubation box of different size; Hatch box further groove design realization simultaneously and hatch a plurality of films of same antibody condition simultaneously; Save antibody incubation liquid, save operator's experimental period, make on the film antibody incubation evenly, fully, the test good reproducibility.

Background technology

At present, protein electrophoresis is a widely used method in the molecular biology, in order to the variation of protein level in the detection of biological sample.In the process of entire method, required 96 orifice plates of using of protein quantification, electrophoresis and change electrophoresis tank, electric turn trough and the electrophoresis/electroporation that film is used, make the albumen colour developing and detect need with compressing tablet box or fluorescence recognition machine all be standardization, and used by vast biological worker.Unified standard like this, the repeatability that makes experiment, repeatability is very high.Yet in the important step of changeing between film and the detection albumen, antibody incubation operation, the ununified form of optimizing.At present many methods of usefulness are to hatch box to hatch with hybridization bag and hatch.Hatching box does not often have specially suitable specification, and small goods box is on the market bought in the laboratory that has, or circular double dish commonly used is hatched antibody in the laboratory.Here the problem of Cun Zaiing is, no matter is that commercialization box or circular double dish are improper in order to hatch film specification disease, waste antibody; The method of hybridization bag is relatively saved antibody, but in sealing hybridization bag process, is easy to generate numerous air-bubble; And the liquid fluidity in the hybridization bag is bad, particularly for the many tests of last sample swimming lane, very easily causes antibody incubation inhomogeneous, causes that repeatability is bad as a result, even false positive, and false negative influences the scientific research interpretation of result.

Summary of the invention

The utility model has designed the antibody incubation box of special requirement, is fit to the antibody incubation of different size film in the actual tests, makes the albumen on the film when saving antibody incubation liquid, and antibody incubation evenly, fully; The antibody incubation box of design band groove is hatched the different films of hatching the same terms antibody incubation liquid in the box simultaneously at one in addition, avoids operate miss, increases work efficiency.

Technical scheme

The technical scheme that its technical matters that solves the utility model adopts is: the last batten band different according to protein electrophoresis in the experiment of various objectives, 2 kinds of length of the utility model design, 2 kinds of width hatch box.The specification of hatching the box internal diameter is respectively (the wide * height of long *, centimetre) S1:9.5*7*3; S2:7*5.87*3; S3:9.5*3.5*3; S4:5.87*3.5*3.In the design of per 1/4 width place three grooves that can insert baffle plate are arranged respectively among the S1, box is separated into 2,3 or 4 cellular-type boxes.Design has pierced pattern above the groove plate (the wide * of long * is thick, 9.7*3.1*0.1 centimetre), make the liquid of hatching in the different compartments of box to circulate, but film but can not circulate, in order to hatch same antibody simultaneously, the parallel experiment of different films.Each box all is furnished with corresponding lid, guarantees to hatch for a long time in the process not loss of liquid in the box.

The concrete technical scheme of the utility model is:

A kind of antibody incubation box, albumen contain film and immerse the antibody incubation liquid of hatching in the box, binding antibody, hatching box is made up of box body, lid and at least one plate, described lid is covered on the box body, is provided with three grooves in this box body, and described plate is inserted in the described groove.

Described antibody incubation box, wherein: hollow out in the middle of the described separation plate.

Beneficial effect

The beneficial effects of the utility model are that (1) different size antibody incubation box is fit to the protein electrophoresis test of not the same batten band number and target protein, saves antibody, saves cost; (2) hatch the box further groove and separate design, realize hatching many films simultaneously, raise the efficiency, save antibody, save cost; (3) boxlike is hatched, and it is evenly complete to make on the film that albumen is hatched antibody incubation, as a result the reappearance height.

Description of drawings

Fig. 1 is that box is hatched in the utility model band partition groove design, box body part outboard profile, and length all is centimetre among the figure:

A, the outer path length (9.5+0.3+0.3) 10.1 of box body;

B, wide (7+0.3+0.3) 7.6 of box body external diameter;

C, box body external diameter height (3+0.2) 3.2;

D, box body and lid anastomosis are high by 0.5;

Path length 9.5 in the e, box body;

F, box body internal diameter is wide by 7;

G, box indent groove width 0.1;

Fig. 2 is that the utility model is hatched box, lid part outboard profile:

Path length (9.5+0.2+0.2) 9.9 in a, lid;

B, wide (7+0.2+0.2) 7.4 of lid internal diameter;

C, lid internal diameter height (0.5+0.3) 0.8;

Fig. 3 is that the utility model is hatched box interpolation groove plate outboard profile:

A, the outer path length (9.5+0.1+0.1) 9.7 of groove plate;

B, wide (3.0+0.1) 3.1 of groove plate external diameter;

C, groove plate thick 0.1;

D, connection strap is wide by 0.53 up and down for the groove plate;

E, the groove plate is connection strap spacing (0.53*2) 1.06 up and down;

F, the groove plate is connection strap and plate limit spacing 0.53 up and down.

The different size of separating design with groove is hatched box with Fig. 1 is similar, and specifically size can be with reference to the data in the technical scheme.

Embodiment

The utility model has designed the antibody incubation box of different size and band groove separation plate.Concrete use as followsly, if need hatch antibody to whole the film that all bands are all gone up sample, employing S1 specification is hatched box; If whole film only having gone up the part band hatched antibody, adopt the S2 specification to hatch box; If the part film to all bands is hatched antibody, adopt the S3 specification to hatch box; If the part film to sample on the part band is hatched antibody, adopt the S4 specification to hatch box; Simultaneously a plurality of part films are hatched antibody if desired, adopt the S1 or the S3 specification that have groove design to hatch box.According to the size of the film of concrete experiment, select the suitable position of hatching box and groove plate.Between the test, will hatch box and clean, prevent the antigen-antibody cross reaction.

Claims

1. antibody incubation box, albumen contains film and immerses the antibody incubation liquid of hatching in the box, binding antibody, it is characterized in that, hatching box is made up of box body, lid and at least one separation plate, described lid is covered on the box body, is provided with three grooves in this box body, and described plate is inserted in the described groove.

2. antibody incubation box according to claim 1 is characterized in that, the described box box body internal diameter of hatching is long * 7 wide * 3 height of S1:9.5; Have three can insert the groove of separating plate in the design of per 1/4 width place respectively among the S1, the separation plate inserts described groove box is separated into 2,3 or 4, and size is respectively long * 5.87 wide * 3 height of S2:7; Long * 3.5 wide * 3 height of S3:9.5; The box body of the long * 3.5 wide * 3 high internal diameters of S4:5.87.

3. antibody incubation box according to claim 1 is characterized in that, hollow out in the middle of the described separation plate.