CN220552863U - Antibody incubation tube for WB experiment - Google Patents

Abstract

The utility model provides an antibody incubation tube for WB experiments, and relates to the field of experimental equipment. This an antibody for WB experiments incubates pipe includes body and baffle, and the number of baffle is N, the baffle set up in the body and separate the body for N+1 mutually independent incubation chamber, the top of body is provided with the opening that is used for putting into the solid phase carrier membrane and incubates the chamber. According to the utility model, one tube body is split into a plurality of incubation cavities, so that the incubation volume of the tube body is reduced, and the dosage of the antibody is reduced compared with that of a conventional antibody incubation box when the same volume of the solid phase carrier film is adopted for incubation. The same space was used for more antibody species than a conventional 50ml centrifuge tube incubated at the same time. And tubular structure can adopt fixed knot structure such as test-tube rack to realize centre gripping and fixed, is difficult to fall from shaking table at incubation in-process, effectively guarantees the accuracy of incubation result. The utility model can be reused and has lower cost.

Description

Antibody incubation tube for WB experiment

Technical Field

The utility model relates to the field of experimental equipment, in particular to an antibody incubation tube for WB experiments.

Background

Western Blot (WB) as one example. It is a common experimental method in molecular biology, biochemistry and immunogenetics. The basic principle is that after the protein is separated by electrophoresis, the protein is transferred to a solid carrier (such as PVDF film), the solid carrier adsorbs the protein in a non-covalent bond form, and the type of the polypeptide separated by electrophoresis and the biological activity thereof are kept unchanged. A protein detection technique for detecting the protein component expressed by specific target gene separated by electrophoresis by using the protein or polypeptide on solid-phase carrier as antigen, immunoreaction with the corresponding antibody, reaction with the enzyme or isotope labeled secondary antibody and substrate development or autoradiography.

WB is a complex procedure and requires high technical requirements. The method has large dosage of antibody and high quality requirement. The incubation of the whole conventional solid support membrane usually requires 6ml to 10ml of antibody dilution, and the amount of antibody used is at least 6 to 10ul when diluted according to the international general 1:1000 ratio.

In the working process, the solid-phase carrier film is often cut off in order to save the antibody due to the high price of the antibody, and different antibodies are respectively incubated in the experimental process. The existing antibody classification incubation box for experiments is larger and higher in incubation box, has large requirements on the area of a shaking table, is square, is directly placed on the shaking table, and easily slides down from the shaking table in the incubation process to cause experimental failure. The antibody incubators currently commercialized also have large amounts of antibody used, usually requiring 3-5ml of antibody dilution (3-5 ul of antibody used). By observation, some laboratories replace the antibody incubation cassette with a 50ml centrifuge tube, but only one antibody can be incubated at a time, wasting space considerably.

In view of this, the present utility model has been made.

Disclosure of Invention

The utility model aims to provide an antibody incubation tube for WB experiments, which can simultaneously realize incubation of a plurality of antibodies, has small antibody consumption, and can meet the incubation requirement by not less than 1mL of liquid in a single cavity, namely according to the following steps of 1: the amount of antibody was 1. Mu.L based on the amount of 1000.

Embodiments of the present utility model are implemented as follows:

in a first aspect, the utility model provides an antibody incubation tube for WB experiments, which comprises a tube body and N partition boards, wherein the partition boards are arranged in the tube body and divide the tube body into n+1 incubation cavities which are independent of each other, and an opening for placing a solid phase carrier film into the incubation cavities is arranged at the top of the tube body.

In an alternative embodiment, the partition is a straight plate or an arcuate plate.

In an alternative embodiment, a plurality of the arcuate plates are tangential and have a center of a circle directed away from the axis of the tube.

In an alternative embodiment, the antibody incubation tube for WB experiments further comprises a cover detachably connected to the tube for opening and closing the opening.

In an alternative embodiment, the cover is hinged to the tube by a snap fit.

In an alternative embodiment, a sealing ring corresponding to the pipe body is arranged in the cover body, and a sealing rubber strip corresponding to the partition plate is further arranged in the cover body.

In an alternative embodiment, a frosted label area is arranged on the surface of the cover body or the outer side surface of the tube body at a position corresponding to each incubation cavity, and the frosted label area can be selectively attached with a label sticker.

In an alternative embodiment, the number of baffles is 2-4.

In alternative embodiments, the volumes of a plurality of said incubation cavities are equal or unequal; the volume of the solid support membrane within each of the incubation chambers is uniform or non-uniform.

In an alternative embodiment, the antibody incubation tube for WB experiments has a height of 100-150mm and a diameter of 25-40mm.

The embodiment of the utility model has the beneficial effects that:

the utility model provides an antibody incubation tube for a WB experiment, which is characterized in that a plurality of partition plates are arranged in the tube body, the partition plates can divide the tube body into a plurality of incubation cavities which are mutually independent, and because in the embodiment, one tube body is divided into a plurality of incubation cavities, the incubation volume of the tube body is reduced, and therefore, when the same volume of a solid phase carrier film is adopted for incubation, compared with a conventional antibody incubation box, the antibody incubation tube for the WB experiment provided by the utility model reduces the dosage of antibodies. In the same space, the antibody incubation tube for WB experiments provided by the utility model has more antibody species than a conventional 50ml centrifuge tube which is incubated at the same time. In addition, the antibody incubation tube for the WB experiment is of a tubular structure, and can be clamped and fixed by adopting a fixing structure such as a test tube rack in the incubation process, so that the antibody incubation tube is not easy to fall from a shaking table in the incubation process, and the accuracy of an incubation result is effectively ensured. The antibody incubation tube for WB experiment can be reused and has lower cost.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic structural diagram of a tube body of an antibody incubation tube for WB experiments according to an embodiment of the present utility model;

fig. 2 is a schematic structural diagram of an antibody incubation tube for WB experiments according to an embodiment of the present utility model when the tube body and the cap body are mated;

fig. 3 is a schematic structural diagram of a cover of an antibody incubation tube for WB experiments according to an embodiment of the present utility model.

Icon: 100-antibody incubation tube for WB experiments; 110-a tube body; 111-opening; 112-a frosted label zone; 113-labeling the sticker; 120-separator; 121-incubation cavity; 130-cover; 131-a buckle; 132-a sealing ring; 133-sealing rubber strips; 140-solid support membrane.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Examples

Referring to fig. 1 and 2, the present embodiment provides an antibody incubation tube 100 for WB experiments, which includes a tube body 110, a partition 120 and a cover 130.

The tube 110 is generally cylindrical, and can be better clamped, so that falling from the shaker during incubation can be effectively avoided, and incubation results are affected. In this embodiment, the top of the tube 110 is provided with an opening 111 for placing the solid support membrane 140 into the tube 110. In this embodiment, the tube 110 is a light-transmitting tube, so that the position of the solid support membrane 140 can be observed. The bottom of the tube 110 in this embodiment may be rounded or tapered.

The number of the partition boards 120 is N, preferably 2-4, the partition boards 120 are arranged in the pipe body 110 and divide the pipe body 110 into N+1 mutually independent incubation cavities 121, the volumes of the plurality of incubation cavities 121 are equal or unequal and are mutually sealed, and mutual interference does not exist between the incubation cavities. The method ensures that different kinds of antibodies can not interfere with each other in the process of incubating the antibodies, and reduces the dosage of the antibodies. The solid carrier film 140 can be placed in different incubation cavities 121 through the openings 111 of the tube body 110, and different antibodies are added into different incubation cavities 121, so that multiple antibodies can be incubated simultaneously, and the volume of the solid carrier film 140 in each incubation cavity 121 can be kept consistent or inconsistent, which is determined according to experimental requirements.

In this embodiment, as long as the plate body that can divide the tube body 110 can be used as the partition plate 120 of the present application, in this embodiment, the partition plate 120 may be a straight plate or an arc plate, and preferably, in this application, the partition plate 120 is an arc plate, and multiple arc plates are tangent and the center of a circle is directed towards the direction far away from the axis of the tube body 110. The setting of arc makes the inside wall that incubates chamber 121 smoother, and in shaking table in-process, the motion smoothness degree of antibody is better, can not have obvious contained angle condition, also washs more easily simultaneously, is difficult for remaining.

Referring to fig. 2 and 3, the cover 130 is detachably connected to the tube 110 to open and close the opening 111, and the cover 130 is hinged to the tube 110 through a buckle 131. Specifically, both sides of the cover 130 and both sides of the top surface of the tube 110 are hinged, so that the cover 130 can be turned upside down on the top surface of the tube 110. When the cover 130 is turned away from the top surface of the tube 110, the interior of the tube 110 is communicated with the outside through the opening 111, and objects (the solid phase carrier membrane 140 and the antibody) can be placed into the incubation cavity 121 from the opening 111.

The sealing rings 132 for sealing the incubation cavities 121 are arranged in the cover body 130, when the cover body 130 is turned back to cover the top surface of the tube body 110, the cover body 130 seals the opening 111 arranged at the top end of the tube body 110, and the tube body 110 is completely separated from the outside, so that liquid is prevented from leaking.

In order to enhance the tightness between the cover 130 and the tube 110, the sealing ring 132 corresponding to the tube 110 is arranged on the inner and outer circles of the cover 130, and the sealing rubber strips 133 corresponding to the partition 120 are arranged on the inner circle according to the separation of the partition 120, so that the tightness between the cover 130 and the tube 110 is better, and the antibodies are prevented from flowing from the cover 130 and the tube 110 to other incubation cavities 121 or interfering with the outside due to the circulation. The tops of the plurality of partition plates 120 are contacted with the sealing rubber strips 133, so that air cannot enter the plurality of incubation cavities 121, and the conditions that liquid in the plurality of incubation cavities 121 leaks and the incubation results are affected by mutual interference are avoided.

A frosted label region 112 is provided on the surface of the cover 130 or the outer side of the tube 110 at a position corresponding to each incubation cavity 121, and a marking sticker 113 may be selectively attached to the frosted label region 112. The arrangement of the frosted label area 112 is convenient for tearing off the attached label paper 113 attached to the area, and the jelly on the label paper 113 cannot remain on the frosted label area 112, so that the frosted label is convenient to clean and reuse. Specifically, separate into a plurality of chambeies 121 of incubating through baffle 120 in this application with body 110, frosted label district 112 also corresponds the setting a plurality of at the surface of lid 130 and the lateral surface of body 110 for frosted label district 112's position corresponds with the position of incubating chambeies 121, is convenient for instruct relevant information such as antibody, incubation time in the corresponding chambeies 121, and all be provided with frosted label district 112 on lid 130 and body 110 in this application, can realize that lid 130 and body 110 correspond incubate chambeies 121 and keep unanimous, avoid appearing lid 130 and body 110 non-corresponding condition, and then avoid appearing the condition of different antibody mutual interference.

The antibody incubation tube 100 used for the WB experiment was 100-150mm in height and 25-40mm in diameter.

Referring to fig. 1, 2 and 3 in combination, an antibody incubation tube 100 for WB experiments works on the following principle:

before the antibody incubation, the operator turns over the cover 130 and puts the solid carrier membrane 140 into the tube body 110 from the opening 111, and in this embodiment, the solid carrier membrane 140 is NC, PVDF or nylon membrane, and different solid carrier membranes 140 are selected according to different requirements of the experiment. The solid phase carrier film 140 is tiled on different tube surfaces of the fixed partition board 120, and the tube body 110 is made of transparent materials, so that the specific position of the solid phase carrier film 140 can be observed, different target antibodies are respectively placed in each incubation cavity 121 when the antibody incubation is carried out, the cover body 130 is turned back to cover the top surface of the tube body 110, and the opening 111 arranged at the top end of the tube body 110 is closed by the buckle 131. At this time, the sealing ring 132 and the sealing rubber strip 133 can well seal each incubation cavity 121 in the tube body 110, so that the incubation cavity is not communicated with the outside, and is not communicated with and interfered with other incubation cavities 121. Meanwhile, corresponding information can be recorded on the label sticker 113 on the frosted label area 112 corresponding to each incubation cavity 121 aiming at different antibodies of different incubation cavities 121, so that operators can conveniently connect the conditions of the corresponding incubation cavities 121 in time. After incubation, the label decal 113 on the frosted label area 112 may be removed and the inside and outside of the entire tube 110 cleaned for subsequent reuse.

In summary, the present utility model provides an antibody incubation tube 100 for WB experiments, in which a plurality of separators 120 are disposed in a tube 110, and the separators 120 can divide the tube 110 into a plurality of incubation chambers 121 that are independent of each other, because in this embodiment, one tube 110 is split into a plurality of incubation chambers 121, so that the incubation volume of the tube 110 is reduced, and when the same volume of solid carrier membrane 140 is used for incubation, compared with a conventional antibody incubation box, the antibody incubation tube 100 for WB experiments provided in the present utility model reduces the amount of antibody, and the liquid amount of a single chamber is not less than 1mL, so that the incubation requirement can be satisfied, namely, according to 1: the amount of antibody was 1. Mu.L based on the amount of 1000. In the same space, the antibody incubation tube 100 for WB experiments provided by the present utility model is more abundant than conventional 50ml centrifuge tubes incubated simultaneously. In addition, the antibody incubation tube 100 for the WB experiment provided by the utility model is of a tubular structure, and can be clamped and fixed by adopting a fixing structure such as a test tube rack in the incubation process, so that the antibody incubation tube is not easy to fall from a shaking table in the incubation process, and the accuracy of an incubation result is effectively ensured. And the antibody incubation tube 100 for WB experiments provided by the utility model can be reused, and the cost is lower.

The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (10)

1. The antibody incubation tube for the WB experiment is characterized by comprising a tube body and N partition plates, wherein the partition plates are arranged in the tube body and divide the tube body into N+1 incubation cavities which are independent of each other, and an opening for placing a solid phase carrier film into the incubation cavities is formed in the top of the tube body.

2. An antibody incubation tube for WB experiments as claimed in claim 1, wherein the spacer is a straight or arcuate plate.

3. An antibody incubation tube for WB experiments as claimed in claim 2, wherein a plurality of said arcuate plates are tangential and have a centre directed away from the axis of the tube.

4. An antibody incubation tube for WB experiments as claimed in claim 1, further comprising a cover detachably connected to the tube to open and close the opening.

5. An antibody incubation tube for WB experiments as claimed in claim 4, wherein the cover is hinged to the tube by a snap fit.

6. An antibody incubation tube for WB experiments as defined in claim 4, wherein a sealing ring corresponding to the tube body is provided in the cover body, and a sealing rubber strip corresponding to the partition plate is further provided in the cover body.

7. An antibody incubation tube for WB experiments as claimed in claim 4, wherein the surface of the cover or the outer side of the tube is provided with a frosted label zone corresponding to each of the incubation cavities, the frosted label zone being selectively attachable with a label sticker.

8. An antibody incubation tube for WB experiments as claimed in any one of claims 1-7, wherein the number of baffles is 2-4.

9. An antibody incubation tube for WB experiments according to any one of claims 1-7, wherein the volumes of a plurality of said incubation chambers are equal or unequal; the volume of the solid support membrane within each of the incubation chambers is uniform or non-uniform.

10. Antibody incubation tube for WB experiments according to any of claims 1-7, characterized in that the height of the antibody incubation tube for WB experiments is 100-150mm and the diameter is 25-40mm.