CN203732541U - ELISA plate with pore bottom partition function - Google Patents

ELISA plate with pore bottom partition function Download PDF

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Publication number
CN203732541U
CN203732541U CN201420063662.9U CN201420063662U CN203732541U CN 203732541 U CN203732541 U CN 203732541U CN 201420063662 U CN201420063662 U CN 201420063662U CN 203732541 U CN203732541 U CN 203732541U
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CN
China
Prior art keywords
hole
elisa plate
interlayer
subregion
divided
Prior art date
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Expired - Fee Related
Application number
CN201420063662.9U
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Chinese (zh)
Inventor
李深伟
刘春梅
鲍晓光
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YOUZRE BIOTECH Co Ltd
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YOUZRE BIOTECH Co Ltd
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Priority to CN201420063662.9U priority Critical patent/CN203732541U/en
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Publication of CN203732541U publication Critical patent/CN203732541U/en
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Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses an ELISA plate with a pore bottom partition function. The ELISA plate comprises an ELISA plate strip, wherein a pore is formed in the ELISA plate strip. The ELISA plate is characterized in that at least one interlayer is formed in the pore; the interior of the pore is divided into multiple containing spaces with the equal volume through the interlayer. The ELISA plate can be used in an experiment for simultaneously detecting multiple (particularly two) indexes. The ELISA plate has the advantages that the pore bottom of the porous plate is divided into two areas, two antibodies or proteins can be simultaneously coated, and the two antibodies or proteins do not interfere with each other during coating. According to the unique porous plate with the pore bottom partition function, multiple indexes are conveniently and simultaneously detected, and the porous plate has the advantages which cannot be compared with a common porous plate.

Description

ELISA Plate that can subregion at the bottom of hole
Technical field
The utility model relates at the bottom of a kind of hole ELISA Plate that can subregion, belongs to immunology detection field.
Background technology
Along with the development of biological medicine science and technology, immunology detection is being brought into play more and more important effect in basic scientific research, clinical practice.Enzyme linked immunosorbent assay (ELISA) ELISA is a detection method important in immunology, in ELISA, as the ELISA Plate of carrier, has at present a unified parameter and standard in the whole world.ELISA Plate apparent size is standardized substantially, is the size of 128x86x15mm substantially.In 96 hole ELISA Plate, the size dimension in each hole is also substantially unified, only hole Bottom Shape can be divided into according to requirement of experiment flat, the U-shaped end.But in 96 at present all hole ELISA Plate, inside, each hole is single, there is no secondary structure.
In detection, to same a sample, need to detect multiple indexs, so just need to carry out repeated detection to sample, need sample size large, the running time is long.The development of detection technique of fluorescence, making to add a sample just can detect many index in a hole becomes possibility.This just need to, when coated, need to be adsorbed on carrier surface the antibody of several indexs or antigen, but disturb to some extent when coated in existing hole.
Utility model content
The problems referred to above that exist in order to solve prior art, the utility model provides at the bottom of a kind of hole ELISA Plate that can subregion.
To achieve these goals, the utility model adopts following technical scheme:
ELISA Plate that can subregion at the bottom of hole, comprises ELIAS strip, and described ELIAS strip is provided with hole, it is characterized in that, is provided with at least one interlayer in described hole, and described interlayer will be divided into the accommodation space that a plurality of volumes are equal in hole.
Further: at the bottom of described hole, be provided with an interlayer, this interlayer is divided into 2 isometric accommodation spaces at the bottom of by hole.
Further: described hole is flat-bottom structure, hole internal diameter 6.9mm, hole depth 10.7mm, at the bottom of hole, central symmetry axis place is provided with interlayer, and described interlayer length is 6.9mm, and width is 0.5mm, be highly 3.2mm, described interlayer is divided into two disposal areas that volume is 60ul at the bottom of by hole.
Further: described ELISA Plate is 96 hole ELISA Plate.
Further: described ELISA Plate comprises ELISA Plate framework, in described ELISA Plate framework, be provided with the detachable ELIAS strip of 12 row, every row ELIAS strip is provided with 8 detachable holes.
Further: described ELISA Plate and interlayer adopt polystyrene to make.
Compared to existing technology, the utlity model has following advantage: the utility model can be used for detecting in the experiment of many indexes (especially two kinds time) simultaneously.Advantage is to be divided into Liang Ge district at the bottom of this micropore plate hole, can be coated with two kinds of antibody or albumen simultaneously, and the two can phase mutual interference when coated.At the bottom of the hole of this uniqueness, subregion microwell plate is convenient to accurately detect many index simultaneously, is the incomparable advantage of common microwell plate.
Brief description of the drawings
Fig. 1 is 96 hole ELISA Plate structural drawing, in this figure, does not arrange interlayer.
Fig. 2 is 96 hole ELISA Plate structural drawing, in this figure, is furnished with interlayer.
Fig. 3 is the cut-open view of 96 hole ELISA Plate mesopores, and in figure, the position of dotted line is in hole, to add after 150ul liquid, the position of liquid level.
In figure, 1-ELISA Plate framework, 2-ELIAS strip, 3-hole, 4-interlayer.
Embodiment
For technological means, creation characteristic that the utility model is realized, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
The ELISA Plate relating in the present embodiment is 96 hole ELISA Plate.
As shown in Figure 2,3, ELISA Plate that can subregion at the bottom of a kind of hole, described ELISA Plate comprises ELISA Plate framework 1, is provided with the detachable ELIAS strip 2 of 12 row in described ELISA Plate framework 1, every row ELIAS strip is provided with 8 detachable holes 3.In described hole 3, be provided with at least one interlayer 4, described interlayer 4 will be divided into the accommodation space that a plurality of volumes are equal in hole.In the present embodiment, described hole is flat-bottom structure, hole internal diameter 6.9mm, hole depth 10.7mm, at the bottom of hole, central symmetry axis place is provided with interlayer, described interlayer length is 6.9mm, width is 0.5mm, is highly 3.2mm, and described interlayer is divided into two disposal areas that volume is 60ul at the bottom of by hole, single hole max cap. is 400ul, and single hole application of sample amount recommends to be to the maximum 360ul.Described ELISA Plate and interlayer adopt polystyrene to make, and color all adopts black.At the bottom of the hole of this uniqueness, subregion microwell plate is convenient to accurately detect many index simultaneously.
Make mould according to following data demand: material polystyrene, apparent size is 127.8x85.5x14.3mm, color is black.The high 10.7mm in hole in each hole, diameter 6.9mm at the bottom of hole; Interlayer length 6.9mm, width 0.5mm, height 3.2mm.Disposable mold becomes 96 orifice plates.There are 12 row micropores, often show 8 micropores, always have 96 micropores.12 row micropores are dismountable (being that ELIAS strip and ELISA Plate framework are dismountable), while being convenient to detect a small amount of sample, use.8 micropores of every row, also can take separately use (be between hole in every row ELIAS strip and ELIAS strip be dismountable) apart.Therefore this microwell plate is dismountable, easy to use.
Need to illustrate, at the bottom of hole, establish interlayer except upper outside for 96 hole ELISA Plate, also can be used for 16 holes or 48 hole ELISA Plate, at the bottom of hole, except an interlayer is set, also can design polylith, for example, design two, then territory, base area, hole is divided into four accommodation spaces that volume is equal, etc., do not enumerate.
Finally explanation is, above embodiment is only unrestricted in order to the technical solution of the utility model to be described, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement the technical solution of the utility model, and not departing from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of claim scope of the present utility model.

Claims (6)

1. a ELISA Plate that can subregion at the bottom of hole, comprises ELIAS strip, and described ELIAS strip is provided with hole, it is characterized in that, is provided with at least one interlayer in described hole, and described interlayer will be divided into the accommodation space that a plurality of volumes are equal in hole.
2. ELISA Plate that can subregion at the bottom of hole according to claim 1, is characterized in that: at the bottom of described hole, be provided with an interlayer, this interlayer is divided into 2 isometric accommodation spaces at the bottom of by hole.
3. ELISA Plate that can subregion at the bottom of hole according to claim 2, it is characterized in that: described hole is flat-bottom structure, hole internal diameter 6.9mm, hole depth 10.7mm, at the bottom of hole, central symmetry axis place is provided with interlayer, and described interlayer length is 6.9mm, and width is 0.5mm, be highly 3.2mm, described interlayer is divided into two disposal areas that volume is 60ul at the bottom of by hole.
4. ELISA Plate that arbitrarily can subregion at the bottom of described hole according to claim 1~3, is characterized in that: described ELISA Plate is 96 hole ELISA Plate.
5. ELISA Plate that can subregion at the bottom of hole according to claim 4, is characterized in that: described ELISA Plate comprises ELISA Plate framework, in described ELISA Plate framework, be provided with the detachable ELIAS strip of 12 row, every row ELIAS strip is provided with 8 detachable holes.
6. ELISA Plate that can subregion at the bottom of hole according to claim 5, is characterized in that: described ELISA Plate and interlayer adopt polystyrene to make.
CN201420063662.9U 2014-02-13 2014-02-13 ELISA plate with pore bottom partition function Expired - Fee Related CN203732541U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420063662.9U CN203732541U (en) 2014-02-13 2014-02-13 ELISA plate with pore bottom partition function

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420063662.9U CN203732541U (en) 2014-02-13 2014-02-13 ELISA plate with pore bottom partition function

Publications (1)

Publication Number Publication Date
CN203732541U true CN203732541U (en) 2014-07-23

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108562740A (en) * 2018-07-02 2018-09-21 天康生物股份有限公司 ELISA plate liquid level detection device and detector
CN109251842A (en) * 2018-09-10 2019-01-22 宁波金未生物科技有限公司 A kind of high-throughput detection and sorting unit

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108562740A (en) * 2018-07-02 2018-09-21 天康生物股份有限公司 ELISA plate liquid level detection device and detector
CN108562740B (en) * 2018-07-02 2024-04-09 天康生物制药有限公司 ELISA plate liquid level detection device and detector
CN109251842A (en) * 2018-09-10 2019-01-22 宁波金未生物科技有限公司 A kind of high-throughput detection and sorting unit

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20140723

Termination date: 20210213