CN108562740A - ELISA plate liquid level detection device and detector - Google Patents
ELISA plate liquid level detection device and detector Download PDFInfo
- Publication number
- CN108562740A CN108562740A CN201810711502.3A CN201810711502A CN108562740A CN 108562740 A CN108562740 A CN 108562740A CN 201810711502 A CN201810711502 A CN 201810711502A CN 108562740 A CN108562740 A CN 108562740A
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- elisa plate
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- liquid level
- detection device
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- 238000002965 ELISA Methods 0.000 title claims abstract description 72
- 239000007788 liquid Substances 0.000 title claims abstract description 65
- 238000001514 detection method Methods 0.000 title claims abstract description 50
- 238000005192 partition Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims description 7
- 238000002474 experimental method Methods 0.000 abstract description 6
- 239000000243 solution Substances 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000003491 array Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
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- 229920003023 plastic Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
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- 210000002966 serum Anatomy 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
- G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
- G01F23/28—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring the variations of parameters of electromagnetic or acoustic waves applied directly to the liquid or fluent solid material
- G01F23/284—Electromagnetic waves
- G01F23/292—Light, e.g. infrared or ultraviolet
- G01F23/2921—Light, e.g. infrared or ultraviolet for discrete levels
- G01F23/2928—Light, e.g. infrared or ultraviolet for discrete levels using light reflected on the material surface
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
The present invention provides a kind of ELISA Plate liquid level detection device and detectors, are related to the technical field of experiment equipment, including placement section and light emitter；Placement section is provided with multiple housing units, and multiple housing units are corresponding with the micro-reaction pond of ELISA Plate respectively；Light emitter includes：Holding tank, top plate, partition board, light source and control switch；The open end of holding tank is connect with top plate；Multiple partition boards are used to receiving cavity being divided into the darkroom unit of multiple strips；Multiple light holes are provided on top plate, and the light hole on each darkroom unit top is arranged along the direction parallel with darkroom unit；Placement section is set to side of the top plate far from holding tank；Housing unit is provided with loophole, for by light hole and loophole by light projection to the micro-reaction pond being set in housing unit that light source emits, to observe the level condition in each micro-reaction pond.The level condition of ELISA Plate liquid level detection device detection solution provided by the invention is more convenient, is easy to promote.
Description
Technical field
The present invention relates to the technical fields of experiment equipment, more particularly, to a kind of ELISA Plate liquid level detection device and detection
Device.
Background technology
At labs enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA)
In the process, ELISA Plate plays an important role.ELISA Plate material is generally polystyrene, and when effect need to be by antigen or antibody with certain
In concentration coating to the micro-reaction pool wall of ELISA Plate, using a variety of methods such as indirect, competitions, pass through colour developing, microplate reader surveys OD
Value detects the target substance in serum, cell culture fluid, body fluid etc., you can and it is qualitative also to quantify, it is most common at present immune
Learn detection means.ELISA Plate can be divided into 48 holes, 96 holes according to its hole count.General is commonly 96 holes.In numerous colleges and universities and section
Grind mechanism, bio-engineering corporation is used widely.
96 hole elisa Plates include 96 micro-reaction ponds, and 96 micro-reaction ponds are in the matrix arrangement of 12*8.96 ELISA Plates
Including fixed ELISA Plate and removable ELISA Plate.Multiple micro-reaction ponds of removable ELISA Plate are uniformly arranged on supporting plate
On, and be detachably connected with supporting plate.
Reaction solution in 96 hole elisa Plates is mostly transparency liquid.Liquid feeding is carried out to the micro-reaction pond in 96 hole elisa Plates
Afterwards, whether the amount of liquid for needing to observe in the mutually micro-reaction pond of colleague or same column is consistent.In the prior art, usually in sample
Colour reagent is added in dilution, whether liquid volume added is consistent to judge the reaction solution in micro-reaction pond, or whether there are leakage feelings
Condition (is likely to occur leakage plus phenomenon) during liquid feeding in the micro-reaction pond having.
Currently, some laboratories are all made of the addition colour developing examination in coating buffer, sample diluting liquid, secondary antibody diluent both at home and abroad
The method of agent judges in micro-reaction pond whether liquid volume added is consistent, whether has leakage hole phenomenon, but for this laboratory knowledge
The protection of property right, each laboratory colour reagent formula secrecy, is unfavorable for the popularization of detection experiment.
Invention content
The purpose of the present invention is to provide a kind of ELISA Plate liquid level detection device and detectors, to alleviate in the prior art not
The technical issues of easily determining the liquid level and leakage hole situation in the micro hole of ELISA Plate.
The present invention provides a kind of ELISA Plate liquid level detection devices, including placement section and light emitter；
The placement section is provided with multiple housing units, multiple housing units respectively with the micro-reaction pond of ELISA Plate
It is corresponding, so that the housing unit can correspond to the multiple micro-reaction ponds for accommodating ELISA Plate；
The smooth emitter includes：Holding tank, top plate, partition board, light source and control switch；The open end of the holding tank and institute
Top plate connection is stated, to form receiving cavity；The quantity of the partition board and light source is disposed as multiple；Multiple partition boards are along default
Direction is set in the receiving cavity, the darkroom unit for the receiving cavity to be divided into multiple strips；Multiple institutes
It states light source to be respectively arranged in the darkroom unit, and the light source is arranged along the extending direction of the darkroom unit；The top
Multiple light holes are provided on plate, multiple light holes are corresponding with the darkroom unit respectively, and each darkroom is single
The light hole on first top is arranged along the direction parallel with the darkroom unit；The placement section is set to the top plate far from described
The side of holding tank；The housing unit is provided with loophole, each loophole of the light hole with the housing unit
It is corresponding, for by the light hole and loophole by light projection that the light source emits to being set in the housing unit
In micro-reaction pond；To observe the level condition in each micro-reaction pond；
The control switch is electrically connected with multiple light sources, for the corresponding opening and closing for controlling the light source.
Further, the inner wall of the housing unit is provided with lighttight light shield layer；The light source is set as monochromatic light
Source.
Further, the color of the light source in the adjacent darkroom unit differs.
Further, multiple housing units are arranged along first direction and second direction；The first direction and second
Direction is vertical；Quantity of the quantity of housing unit arranged in the first direction more than the housing unit of second direction.
Further, multiple housing units are arranged along first direction and second direction；The first direction and second
Direction is vertical；The quantity of the housing unit arranged positioned at the first direction and second direction is identical, and positioned at first direction and
The maximum number of the quantity of housing unit and the micro-reaction pond of the first direction of ELISA Plate and second direction of second direction arrangement
It measures identical.
Further, the quantity of the housing unit of the first direction and second direction of the placement section is disposed as 12.
Further, the smooth emitter include with for the limiting section that is connect with the placement section so that the light hole
It is corresponding with the loophole of the housing unit.
Further, the top plate and the holding tank are detachably connected.
Further, the edge of the placement section is provided with recess portion, and gap is formed between ELISA Plate and recess portion for making.
The present invention also provides a kind of detectors, including cover board and ELISA Plate liquid level detection device as described above；
The cover board is made of clear material, and the cover board is provided with multiple liquid filling holes, multiple liquid filling hole difference
It is corresponding with the housing unit, for that will suck or be sucked out culture solution by the cover board.
When detection, ELISA Plate is positioned in housing unit for ELISA Plate liquid level detection device provided by the invention, and light source is logical
It crosses light hole, loophole and forms reflecting surface on the liquid level in micro-reaction pond, observation can be facilitated micro anti-by reflecting surface
Level condition in Ying Chi.If being included in same amount of liquid in micro-reaction pond, the light reflection surface in micro-reaction pond is answered
Unanimously, and overlook and slightly inclined direction observe its reflecting surface be in same horizontal plane.If micro-reaction pond is without light reflection
Face is less than average reflection face, then is judged as that leakage hole or sample-adding amount are inconsistent, so as to by fluid infusion mode, so that each micro
Liquid volume added is consistent in reaction tank.Since the micro-reaction pond of corresponding column (or row) can be observed when opening by open light source
Liquid level situation, with timely fluid infusion, therefore detect more convenient, and judge that level condition is more accurate.Compared with the prior art
In be detected by colour reagent, level condition cashier's production of ELISA Plate liquid level detection device provided by the invention detection solution
It is more convenient, it is easy to promote.
Detector provided by the invention, including cover board and ELISA Plate liquid level detection device as described above；The cover board by
Transparent material is made, and the cover board is provided with multiple liquid filling holes, and multiple liquid filling holes are corresponding with the housing unit respectively,
For that will suck or be sucked out culture solution by the cover board.Detector provided by the invention detects the level condition of solution more
It is convenient, it is easy to promote.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in being described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, other drawings may also be obtained based on these drawings.
Fig. 1 is the schematic diagram of ELISA Plate liquid level detection device provided in an embodiment of the present invention；
Fig. 2 is the vertical view of ELISA Plate liquid level detection device provided in an embodiment of the present invention；
Fig. 3 is the schematic cross-section of the parts A-A in Fig. 2；
Fig. 4 is the vertical view of the placement section in ELISA Plate liquid level detection device provided in an embodiment of the present invention；
Fig. 5 is the schematic diagram of the light emitter in ELISA Plate liquid level detection device provided in an embodiment of the present invention；
Fig. 6 is the schematic diagram of the holding tank in detector provided in an embodiment of the present invention.
Icon：1- placement sections；2- light emitters；11- housing units；111- loopholes；21- holding tanks；22- light
Source；23- control switches；24- partition boards；25- top plates；The darkrooms 211- unit；251- light holes.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
The every other embodiment that personnel are obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that such as occur term "center", "upper", "lower", "left", "right",
"vertical", "horizontal", "inner", "outside" etc., indicated by orientation or positional relationship be orientation based on ... shown in the drawings or position
Relationship is merely for convenience of description of the present invention and simplification of the description, and not indicating or implying the indicated device or element must have
There is specific orientation, with specific azimuth configuration and operation, therefore is not considered as limiting the invention.In addition, as occurred
Term " first ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " peace such as occur
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete condition
The concrete meaning of language in the present invention.
As shown in Figure 1, Figure 2 and Figure 3, ELISA Plate liquid level detection device provided in this embodiment includes that placement section 1 and light are penetrated
Device 2；Placement section 1 is provided with multiple housing units 11, and multiple housing units 11 are corresponding with the micro-reaction pond of ELISA Plate respectively.
Since the micro-reaction pond in 96 hole elisa Plates in the prior art is generally matrix arrangement, multiple housing units 11 can be with
It is arranged in arrays, so that multiple housing units 11 can accommodate multiple micro-reaction ponds of ELISA Plate.Light emitter 2 is for generating
Transmitted light is simultaneously refracted in the micro-reaction pond of 96 hole elisa Plates.Light emitter 2 may include：Holding tank 21, top plate 25, it is multiple every
Plate 24, multiple light sources 22 and control switch 23.Multiple partition boards 24 are set to along preset direction in receiving cavity, are used for accommodating chamber
Body is divided into the darkroom unit 211 of multiple strips, as shown in Figure 6；Multiple light sources 22 are respectively arranged in darkroom unit 211,
And light source 22 is arranged along the extending direction of darkroom unit 211；The top of each darkroom unit 211 (is provided with light extraction on top plate 25
Hole 251), the multiple light holes 251 parallel with 211 direction of darkroom unit are both provided with, placement section 1 is set to 2 top of light emitter,
The bottom of housing unit 11 is provided with loophole 111, the loophole of multiple light holes 251 and 11 bottom of corresponding housing unit
111 is corresponding, for passing sequentially through light hole 251, loophole 111, by the light projection of light source 22 to being positioned over housing unit 11
In interior micro-reaction pond；Control switch 23 is electrically connected with multiple light sources 22, the opening and closing for corresponding control light source 22.
When detection, ELISA Plate is positioned in housing unit 11, opens the light source 22 in a darkroom unit 211, strip
Light source 22 pass sequentially through light hole 251, loophole 111 enters in housing unit 11, and the liquid level in micro-reaction pond
One column (or row) reflecting surface of upper formation can facilitate the level condition in observation micro-reaction pond by reflecting surface.If micro anti-
Same amount of liquid is included in Ying Chi, then the light reflection surface in micro-reaction pond should be consistent.If unglazed in micro-reaction pond
Reflecting surface is less than average reflection face, then is judged as that leakage hole or sample-adding amount are inconsistent, needs to carry out fluid infusion at this time, until each micro
The sample-adding amount of liquid in reaction tank is consistent.When detection, different light sources 22 can be opened successively, to be examined in positioned at identical
Multiple light sources 22 can also be opened simultaneously and are observed by the level condition in the micro-reaction pond on strip darkroom unit 211.Cause
This, the detection in the ELISA Plate liquid level detection device provided in this embodiment micro-reaction pond single-row to ELISA Plate successively is more square
Just, and judge that level condition is more accurate.It is detected by colour reagent in compared with the prior art, when addition colour reagent
Afterwards, irreversible change is caused to solution dyeing so that the level condition of the respective column detected before is easier to influence next column
Solution level condition.Meanwhile ELISA Plate liquid level detection device provided in this embodiment, it need to need not be in micro-reaction pond
Colour reagent etc. is added, the level condition for detecting solution is more convenient, is easy to promote.
Above-mentioned placement section 1 can be made of light-proof material.Placement section 1 can be lighttight plastics or stainless steel etc.
Material is made.Alternatively, the inner wall of housing unit 11 can be provided with lighttight light shield layer, to avoid outer bound pair ELISA Plate
Light projection ring.Light source 22 could be provided as monochromatic source 22, and to avoid occurring during light reflection, formation after optical dispersion is more
Color liquid level.
Further, the color of the light source 22 in adjacent darkroom unit 211 differs.Above-mentioned setting, can while will be more
Corresponding light source 22 is opened in a darkroom unit 211, to observe the level condition in the micro-reaction pond of multiple row on ELISA Plate, to carry
High detection efficiency.
Multiple housing units 11 could be provided as：Multiple housing units 11 are arranged along first direction and second direction；First
Direction and second direction are vertical；The quantity of housing unit 11 arranged in the first direction is more than the housing unit 11 of second direction
Quantity.In an experiment, the ELISA Plate generally used is 96 hole elisa Plates, and the micro-reaction pond of 12*8 is up on ELISA Plate.It is micro-
Quantitative response pond can be detachably connected on the supporting plate of ELISA Plate, and it is multiple micro- therefore, on ELISA Plate can also to place 12*4 etc.
Quantitative response pond.Therefore, in a first direction, housing unit 11 can with quantity could be provided as 12；It is accommodated in second direction
Unit 11 can with quantity could be provided as 8, to meet detection needs.The darkroom unit 211 of above-mentioned second direction and strip
Extending direction it is parallel.
For needing to detect in the mutually experiment of colleague and same column, ELISA Plate liquid level detection device provided in this embodiment
It could be provided as：Multiple housing units 11 are arranged along first direction and second direction；It is arranged positioned at first direction and second direction
Housing unit 11 quantity it is identical, and the quantity and ELISA Plate of the housing unit 11 arranged positioned at first direction and second direction
First direction it is identical with the maximum quantity in the micro-reaction pond of second direction.For being placed with the ELISA Plate of 12*8, work as detection
Complete ELISA Plate arrange to micro-reaction pond level condition, ELISA Plate can be rotated by 90 ° along its horizontal direction, with to ELISA Plate
Line direction micro-reaction pond in liquid level be detected.
By taking common 96 hole elisa Plates in experiment as an example, as shown in Figure 4, Figure 5,96 hole elisa Plates holes can be at most arranged
The micro-reaction pond of 12*8, therefore, in ELISA Plate liquid level detection device provided in this embodiment, the first direction of plate placement section 1
It is disposed as 12 with the quantity of the housing unit 11 of second direction.ELISA Plate is rotated after the row to ELISA Plate are detected
After 90 degree, its row can be detected.
Alternatively, multiple housing units 11 are arranged along first direction and second direction, and appearance arranged in the first direction
Receive unit 11 quantity more than second direction housing unit 11 quantity, light hole 251 is arranged in a second direction, and it is multiple go out
The length that unthreaded hole 251 is arranged in order is greater than or equal to the length of the multiple housing units 11 arranged in a second direction.Above-mentioned setting
ELISA Plate can be placed into placement section 1 after detection row, placement section 1 and ELISA Plate be rotated simultaneously, with the row to ELISA Plate
Micro-reaction pond on direction is detected.For example, for 96 hole elisa Plates, placement section 1 can be provided with the receiving list of 12*8
Member 11.In a first direction, light takes the photograph the light hole 251 that plate is provided with 12*12.After the completion of being detected to the column direction of ELISA Plate,
Placement section 1 and ELISA Plate can be rotated together with to 90 degree, then placement section 1 is positioned in light emitter 2, ELISA Plate is examined into every trade
It surveys.
Placement section 1 is put into the top of light emitter 2 for convenience, so that light hole 251 and housing unit 11 correspond to, is made
Light emitter 2 may include the limiting section for being connect with placement section 1.Specifically, can be arranged at the edge on the top of light emitter 2
Protrusion is provided with groove corresponding with protrusion, when the top that placement section 1 is put into light emitter 2 in the downside of placement section 1
When, it can be corresponding with groove by protrusion, quickly light extraction 251 and housing unit 11 to be corresponded to.Meanwhile the setting of protrusion and groove
It can be skidded off from 2 top of light emitter to avoid placement section 1.
Top plate 25 and holding tank 21 could be provided as the mode being detachably connected.Above-mentioned setting can be convenient for changing light emitter
Light source 22 in 2.
It takes for convenience the ELISA Plate being positioned in housing unit 11, the edge of placement section 1 is provided with recess portion, is used for enzyme
Gap is formed between target and recess portion.Operating personnel can conveniently be taken ELISA Plate by above-mentioned gap.
Above-mentioned light source 22 can be the LED light of bar shaped.A diameter of 2-4mm of light hole 251.Receiving on holding tank 21
Unit 11 could be provided as cylindrical shape, and the bottom of housing unit 11 is identical as upper opening size.Above-mentioned setting, with facilitate light from
The bottom of housing unit 11 is irradiated into micro-reaction pond.
Above-mentioned control switch 23 could be provided as it is multiple, multiple control switches 23 successively respectively from different darkroom units 211
Interior light source 22 corresponds to.It includes brightness control unit to control switch 23, and brightness control panel is electrically connected with control switch 23, for controlling
The brightness of light source 22 processed.
In conclusion ELISA Plate liquid level detection device provided in this embodiment, placement section 1 are provided with multiple arranged in arrays
Housing unit 11, housing unit 11 is used to place the micro-reaction pond of ELISA Plate.11 bottom of housing unit is set as loophole
111.The lower part of placement section 1 is light emitter 2.Light emitter 2 includes the darkroom unit 211 of strip, is both provided in darkroom unit 211
The light source 22 of strip.Along the extending direction of darkroom unit 211, unit 211 top in darkroom is provided with multiple light holes 251, more
A light hole 251 is corresponding with the bottom of housing unit 11 respectively.The light that light source 22 generates passes through light hole 251, loophole
111, it is irradiated in the micro-reaction pond in housing unit 11, for forming plane of refraction in micro-reaction pond, passes through refraction
The height in face, with judge row to multiple micro-reaction ponds liquid level condition.Simultaneously control switch 23 control respectively respective column to
Light source 22, when detection can successively to arrange to micro-reaction pond be detected, detection is more convenient, and detection efficiency is higher.
Detector provided in this embodiment, including cover board and ELISA Plate liquid level detection device as above；Cover board is by transparent material
Material is made, and cover board is provided with multiple liquid filling holes, and multiple liquid filling holes are corresponding with housing unit 11 respectively, for that will be inhaled by cover board
Enter or be sucked out culture solution.After detection is just as the liquid level in the micro-reaction pond of row, can by liquid-transfering gun by liquid filling hole to low
The micro-reaction pond of liquid level carries out liquid feeding.Simultaneously because cover board is made of clear material, facilitate the liquid in observation micro-reaction pond
Position situation.When liquid feeding, cover plate lid is closed on ELISA Plate, is avoided during detection and liquid feeding, the impurity such as dust enter
Situation in micro-reaction pond.Detector provided in this embodiment, the level condition for detecting solution is more convenient, is easy to promote.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of ELISA Plate liquid level detection device, which is characterized in that including placement section and light emitter；
The placement section is provided with multiple housing units, and multiple housing units are opposite with the micro-reaction pond of ELISA Plate respectively
It answers, so that the housing unit can correspond to the multiple micro-reaction ponds for accommodating ELISA Plate；
The smooth emitter includes：Holding tank, top plate, partition board, light source and control switch；The open end of the holding tank and the top
Plate connects, to form receiving cavity；The quantity of the partition board and light source is disposed as multiple；Multiple partition boards are along preset direction
It is set in the receiving cavity, the darkroom unit for the receiving cavity to be divided into multiple strips；Multiple light
Source is respectively arranged in the darkroom unit, and the light source is arranged along the extending direction of the darkroom unit；On the top plate
Multiple light holes are provided with, multiple light holes are corresponding with the darkroom unit respectively, and each darkroom unit top
The light hole at end is arranged along the direction parallel with the darkroom unit；The placement section is set to the top plate far from the receiving
The side of slot；The housing unit is provided with loophole, and each light hole is corresponding with the loophole of the housing unit,
For by the light hole and loophole that the light projection that the light source emits is micro in the housing unit to being set to
In reaction tank；To observe the level condition in each micro-reaction pond；
The control switch is electrically connected with multiple light sources, for the corresponding opening and closing for controlling the light source.
2. ELISA Plate liquid level detection device according to claim 1, which is characterized in that the inner wall of the housing unit is arranged
There is lighttight light shield layer；The light source is set as monochromatic source.
3. ELISA Plate liquid level detection device according to claim 2, which is characterized in that the light in the adjacent darkroom unit
The color in source differs.
4. ELISA Plate liquid level detection device according to claim 1, which is characterized in that multiple housing units are along first
Direction and second direction arrangement；The first direction and second direction are vertical；The quantity of housing unit arranged in the first direction
More than the quantity of the housing unit of second direction.
5. ELISA Plate liquid level detection device according to claim 1, which is characterized in that multiple housing units are along first
Direction and second direction arrangement；The first direction and second direction are vertical；It is arranged positioned at the first direction and second direction
Housing unit quantity it is identical, and the of the quantity of the housing unit arranged positioned at first direction and second direction and ELISA Plate
One direction is identical with the maximum quantity in the micro-reaction pond of second direction.
6. ELISA Plate liquid level detection device according to claim 5, which is characterized in that the first direction of the placement section and
The quantity of the housing unit of second direction is disposed as 12.
7. ELISA Plate liquid level detection device according to claim 1, which is characterized in that the smooth emitter include with for
The limiting section of the placement section connection, so that the light hole is corresponding with the loophole of the housing unit.
8. ELISA Plate liquid level detection device according to claim 1, which is characterized in that the top plate and the holding tank can
Dismantling connection.
9. ELISA Plate liquid level detection device according to claim 1, which is characterized in that the edge of the placement section is provided with
Recess portion forms gap for making between ELISA Plate and recess portion.
10. a kind of detector, which is characterized in that including cover board and ELISA Plate liquid level as described in any one of claims 1-9
Detection device；
The cover board is made of clear material, and the cover board is provided with multiple liquid filling holes, multiple liquid filling holes respectively with institute
Housing unit correspondence is stated, for that will suck or be sucked out culture solution by the cover board.
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| CN108562740B (en) | 2024-04-09 |
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