WO2024040922A1

WO2024040922A1 - Platelet testing device

Info

Publication number: WO2024040922A1
Application number: PCT/CN2023/080565
Authority: WO
Inventors: 张传国; 强勇平; 李运奇; 赖鹏飞; 郑凯; 谢明君
Original assignee: 深圳市爱康生物科技股份有限公司
Priority date: 2022-08-24
Filing date: 2023-03-09
Publication date: 2024-02-29
Also published as: CN115327090A

Abstract

Disclosed in the present application is a platelet testing device, comprising: a turntable mechanism on which a carrier assembly is detachably arranged, the carrier assembly comprising a first carrier assembly or a second carrier assembly, the carrier assembly being used for carrying a reagent bottle containing a sample or a corresponding type of reagent, the first carrier assembly being used for performing an antibody testing experiment, and the second carrier assembly being used for performing a cross matching experiment; a centrifugal incubation mechanism, which is arranged on one side of the turntable mechanism, the centrifugal incubation mechanism comprising a centrifugal machine and an incubator, the centrifugal machine being used for centrifuging samples in micro-plate holes, the incubator being used for incubating the samples in the micro-plate holes, and a first window being formed in the upper end of the incubator; a sample injection mechanism, comprising a first driving assembly and a sample injection needle, the first driving assembly being used for driving the sample injection needle to inject samples into the holes; and a determination mechanism. The present application can effectively achieve both the antibody testing experiment and the cross matching experiment by arranging the detachable carrier assembly, thereby reducing the testing cost of platelets.

Description

Platelet detection device

Technical field

The present application relates to the technical field of medical devices, and in particular to a platelet detection device.

Background technique

Platelet transfusion is an effective measure to prevent and treat bleeding caused by thrombocytopenia or functional defects, and to reduce the mortality rate of bleeding caused by thrombocytopenia after radiotherapy and chemotherapy. With the popularity of platelet transfusion in clinical practice, the ineffectiveness of platelet transfusion has gradually attracted people's attention.

At present, platelet detection methods generally include antibody detection and cross-matching. By detecting platelet antibodies, the platelet antibody detection antigen can help doctors find out the reasons for ineffective platelet transfusion; it can also guide doctors to choose appropriate platelet transfusion plans for patients to rationally and effectively utilize precious blood resources; it can also help diagnose repeated transfusions. For patients who receive platelet infusion, the corresponding antibodies in their serum should be measured regularly to predict the effect of platelet transfusion and promptly discover immune factors that lead to ineffective platelet transfusion. Cross-matching can distinguish the type of platelet antibodies in patients; it can also select corresponding antigen-negative platelets for transfusion to improve the effect of platelet transfusion.

Existing platelet antibody detection experiments and cross-matching experiments need to be performed on corresponding antibody detection devices and cross-matching devices respectively. Both platelet detection and cross-matching are required to meet different detection needs, which in turn increases the cost of platelet testing. higher.

Contents of the invention

In order to solve the problem that existing platelet detection devices are not compatible with antibody detection experiments and cross-matching experiments, this application proposes a platelet detection device that can perform both platelet antibody detection experiments and platelet cross-matching experiments, thereby Reduce the cost of platelet testing.

The platelet detection device according to the embodiment of the present application includes: a turntable mechanism on which a carrier assembly is detachably provided. The carrier assembly includes a first carrier assembly or a second carrier assembly, and a test tube hole is provided on the carrier assembly. , the test tube hole is used to place reagent bottles loaded with samples or corresponding types of reagents; wherein, the first carrier component is used to perform antibody detection experiments, and the second carrier component is used to perform cross-matching experiments; centrifugal incubation mechanism , is arranged on one side of the turntable mechanism. The centrifugal incubation mechanism includes a centrifuge and an incubator. The incubator is provided with micro-slats. The centrifuge is provided inside the incubator. The centrifuge is connected to the micro-slats. The micro-slats are provided with There are multiple microplate holes. The centrifuge is used to centrifuge the samples in the microplate holes. The incubator is used to incubate the samples in the microplate holes. A first window is provided at the upper end of the incubator. The sample adding mechanism is set on the turntable mechanism. Above, the sample adding mechanism includes a first driving component and a sample adding needle. The first driving component is used to drive the sample adding needle to add sample into the hole; the interpretation mechanism is set above the turntable mechanism, and the interpretation mechanism is used to analyze the experimental results of the sample. Perform interpretation.

The platelet detection device according to the first embodiment of the present application has at least the following beneficial effects: when performing an antibody detection experiment, the first carrier component can be placed on the turntable mechanism, and the antibodies can be placed at different positions of the first carrier component Samples and reagents required for detection experiments are used to conduct antibody detection experiments; when performing cross-matching experiments, a second carrier component can be placed on the turntable mechanism, and cross-matching laboratories are placed at different positions of the second carrier component Required samples and reagents to perform cross-matching experiments. By arranging a detachable carrier component on the platelet detection device, this application can effectively be compatible with antibody detection experiments and cross-matching experiments, thereby reducing the cost of platelet detection.

According to some embodiments of the present application, the turntable mechanism is an annular hollow structure, the turntable mechanism is provided with a first drive unit and a second drive unit, the carrier assembly is disposed on the outer ring of the turntable mechanism, the first drive unit and the second drive unit Both are connected to the carrier assembly. The incubator is arranged in the middle of the turntable mechanism. The first drive unit is used to drive the turntable mechanism to drive the carrier assembly to rotate to a preset position. The second drive unit is used to drive the reagent bottle to rotate and shake evenly. By setting the turntable mechanism as a ring-shaped hollow structure, placing the incubator in the middle of the turntable mechanism, and arranging the carrier assembly on the outer ring of the turntable mechanism, space can be effectively utilized and the space occupied by the centrifugal incubation mechanism can be reduced without the need for sample addition. At this time, the first driving unit drives the outer ring of the turntable mechanism to rotate and rotates the corresponding sample position to the preset position so that the sample can be processed accordingly. The second driving unit is used to drive the reagent bottle loaded with the reagent to be mixed for rotation and mixing. uniform.

Further, the first carrier assembly includes a reagent carrier and a sample carrier. The reagent carrier and sample carrier required for antibody detection are placed on the outer ring of the turntable mechanism, so that the sample carrier and reagent carrier are rotated to the bottom of the sample loading mechanism respectively, and the samples and reagents are sequentially added to the centrifuge incubator through the sample adding needle. organization to take the next step.

Further, the first carrier assembly includes 4 sample carriers and 1 reagent carrier. By setting 4 sample carriers and 1 reagent carrier, multiple samples can be tested in each batch, effectively improving detection efficiency. efficiency.

According to some embodiments of the present application, the second carrier assembly includes a sample carrier, a reagent carrier and a mixing carrier, and the mixing carrier is connected to the second driving unit. The reagent carrier, sample carrier and mixing carrier required for cross-matching are placed on the outer ring of the turntable mechanism. The mixing carrier is connected to the second drive unit, and the second drive unit drives the mixing carrier to be placed on the outer ring of the turntable mechanism. After the reagents are mixed, the mixed reagents and samples are moved to the centrifugal incubation mechanism for the next step.

Further, the second carrier assembly includes a sample carrier, a reagent carrier, an eight-position mixing carrier and two four-position mixing carriers. By setting up one eight-position mixing carrier and two four-position mixing carriers, multiple sets of reagents can be mixed at the same time, improving the mixing efficiency and thereby improving the experimental efficiency of cross-matching.

According to some embodiments of the present application, the sampling mechanism is provided with two groups of sampling channels, each group of sampling channels is provided with at least one sampling needle and one driving unit, and the two groups of sampling channels are independently lifted and lowered in the vertical direction. sports. By setting up two sets of independently ascending and descending loading channels, two sample positions can be loaded at the same time, thereby improving the loading efficiency and improving the experimental efficiency of platelet antibody detection and/or cross-matching.

According to some embodiments of the present application, the interpretation mechanism is connected to the sample adding mechanism, and the interpretation mechanism includes a fifth driving unit, and the fifth driving unit is used to drive the interpretation mechanism to move in the vertical direction. By connecting the interpretation mechanism with the sample adding mechanism, the interpretation mechanism can be raised and lowered for interpretation while also saving the space occupied by the interpretation mechanism.

Further, the platelet detection device further includes a lever, which is connected to the fifth driving unit, and the lever is used to open or close the first window.

Further, a magnet is provided at the lower end of the lever, and a blocking piece is provided at the upper end of the first window. The magnet is used to cooperate with the blocking piece to open or close the first window.

Furthermore, a sensing component is provided on the interpretation mechanism, and the sensing component is used to sense the spatial position of the interpretation mechanism. By setting up the sensing component, the interpretation mechanism can be accurately positioned to avoid the problem of low interpretation accuracy caused by the failure of the interpretation mechanism to be aligned.

Further, the interpretation mechanism also includes a camera, a camera box and a light-shielding sleeve. The camera is arranged in the middle of the camera box, and the light-shielding sleeve is arranged at the lower end of the camera box. Installing a light-shielding sleeve at the lower end of the camera box can effectively avoid interference from ambient light and improve the accuracy of interpretation of experimental results.

According to some embodiments of the present application, the platelet detection device further includes a plate washing mechanism. The plate washing mechanism includes a plate washing head assembly and a second driving assembly. The second driving assembly is used to drive the plate washing head assembly to perform lifting movements. By setting up the plate washing head assembly, the sampling needle can be cleaned in time, which can save the cost of cleaning agent consumables. While saving costs, it can effectively improve the accuracy of platelet detection experiments.

Further, the board washing head assembly includes at least a set of liquid injection needles and liquid suction needles. The liquid injection needle and the liquid suction needle are provided at the lower end of the board washing head assembly. The liquid injection needle and/or the liquid suction needle are provided with an escape portion. Bend the end toward the middle of the injection needle and the aspiration needle. By arranging the injection needle and the aspiration needle as a group, the injection and aspiration actions can be performed separately in the same microplate well, thus shortening the plate washing time. When the diameter of the microplate hole is small, inserting the injection needle and the pipetting needle into the same microplate hole at the same time will easily cause the phenomenon of needle impact, that is, the injection needle or the pipetting needle hits the edge or inner wall of the microplate hole. Causes cleaning failure. Therefore, this solution can effectively avoid the risk of needle collision by providing an escape portion at a preset position of the injection needle and/or the aspiration needle, and the escape portion is bent toward the middle of the injection needle and the aspiration needle.

Further, a maintenance pool is also provided on one side of the second driving assembly, and the maintenance pool is used for cleaning and maintenance of the washing head assembly. By setting up a maintenance pool for cleaning and maintaining the plate washer head assembly, the plate washer head assembly can be cleaned and maintained in a timely manner to avoid cross-contamination of samples and at the same time improve the service life of the plate washer head assembly.

Further, the washing mechanism also includes a washing head hanger and a washing head hanger. The upper end of the washing head assembly is also provided with a first fixing hole and a first adjustment hole. The washing head assembly passes through the first fixing hole and the first adjustment hole. The hole is fixedly connected to the washing head pendant, and the first adjustment hole is used to adjust the angle of the washing head assembly in the horizontal direction. By arranging the first adjustment hole for adjusting the angle of the washing head assembly in the horizontal direction, the position of the washing head assembly in the horizontal direction can be accurately adjusted, thereby achieving precise positioning of the washing head assembly and the microplate hole, and avoiding inaccurate positioning and The corresponding microplate well cannot be inserted or there is a problem with the striker.

Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.

Description of drawings

In order to more clearly explain the embodiments of the present application or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below. Obviously, the drawings in the following description are only those recorded in the present application. For some embodiments, those of ordinary skill in the art can also obtain other drawings based on these drawings.

Figure 1 is a schematic diagram of the overall structure of a platelet detection device according to an embodiment of the present application;

Figure 2 is a schematic structural diagram of the turntable mechanism of the platelet detection device according to the embodiment of the present application;

Figure 3 is a schematic structural diagram of the turntable mechanism of the platelet detection device according to another embodiment of the present application;

Figure 4 is a partial structural schematic diagram of the turntable mechanism of the platelet detection device according to the embodiment of the present application;

Figure 5 is a schematic structural diagram of the sample adding mechanism and the interpretation mechanism of the platelet detection device according to the embodiment of the present application;

Figure 6 is an exploded view of the interpretation mechanism of the platelet detection device according to the embodiment of the present application;

Figure 7 is a schematic structural diagram of the plate washing mechanism of the platelet detection device according to the embodiment of the present application;

Figure 8 is a schematic structural diagram of the plate washing mechanism of the platelet detection device according to another embodiment of the present application;

Figure 9 is a schematic structural diagram of the plate washing head assembly of the platelet detection device according to the embodiment of the present application;

Figure 10 is a cross-sectional view of the plate washing head assembly of the platelet detection device according to the embodiment of the present application;

Figure 11 is a top view of the plate washing head assembly of the platelet detection device according to the embodiment of the present application.

Reference signs:
Turntable mechanism 100, reagent carrier 110, sample carrier 120, mixing carrier 130, eight-position mixing carrier 131, four-position mixing carrier 132,
The second driving unit 133, the handheld portion 140, the turntable base 150, the carrier base 160, the positioning column 170, the anti-fool column 180, the support column 190, the centrifugal incubation mechanism 200, the incubator 210, the first window 211, and the sampling mechanism 300 , the first sampling channel 310, the first sampling needle 311, the third driving unit 312, the second sampling channel 320, the second sampling needle 321, the fourth driving unit 322, the first fixing member 323, the second fixing 324, the first connecting piece 325, the first adapter 326, the second linear guide rail 327, the fifth drive unit 328, the first linear guide rail 330, the first screw rod 350, the lever 360, the magnet 361, the second Connector 362, limiting nail 363, reading mechanism 400, camera 410, camera box 420, light shielding sleeve 430, camera pendant 440, cover 450, sensing component 460, positioning sensor 461, sensing piece 462, third fixing member 470 , plate washing mechanism 500, plate washing head assembly 510, liquid injection needle 511, liquid suction needle 512, avoidance part 513, first adjustment hole 514, first fixing hole 515, second drive assembly 520, third linear guide rail 530, The second screw rod 540, the hub 550, the maintenance pool 560, the bracket 570, the washing head hanger 580, the washing head hanger 590, and the U-shaped groove 591.

Detailed ways

The embodiments of the present application are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements with the same or similar functions. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application and cannot be understood as limiting the present application.

In the description of this application, it should be understood that the orientation descriptions, such as up, down, left, right, front, back, etc., are based on the orientations or position relationships described in the drawings, and are only for convenience. The present application is described and the simplified description is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore is not to be construed as a limitation on the present application.

In the description of this application, the meaning of plural is two or more, greater than, less than, etc. are understood to exclude the original number, and at least, not greater than, above, below, within, etc. are understood to include the original number. The first, second, etc. described in this application are only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the indicated technology. The sequence of features.

In the description of this application, unless otherwise expressly limited, words such as "set" and "connection" should be understood in a broad sense. Those skilled in the art can reasonably determine the specific meaning of the above words in this application based on the specific content of the technical solution.

With the popularity of platelet transfusion in clinical practice, the ineffectiveness of platelet transfusion has gradually attracted people's attention. In related technologies, platelet antibody detection and cross-matching are generally used to determine the antibody type of platelets to avoid ineffective transfusion. These two experiments require It is performed on the corresponding antibody detection device and cross-matching device respectively. In order to meet different detection needs, it is necessary to have both platelet antibody detection and cross-matching. This will significantly increase the cost of platelet detection.

In order to solve the problem that the platelet detection device is not compatible with antibody detection experiments and cross-matching experiments, this application proposal proposes a platelet detection device that can perform both platelet antibody detection experiments and platelet cross-matching experiments, thereby reducing the risk of platelet loss. Testing costs.

Referring to Figures 1 and 5, the present application proposes a platelet detection device, including: a turntable mechanism 100, on which a carrier assembly is detachably provided. The carrier assembly includes a first carrier assembly or a second carrier assembly. , the carrier component is provided with test tube holes, and the test tube holes are used to place reagent bottles loaded with samples or corresponding types of reagents; wherein the first carrier component is used for antibody detection experiments, and the second carrier component is used for cross Matching experiment; the centrifugal incubation mechanism 200 is arranged on one side of the turntable mechanism 100. The centrifugal incubation mechanism 200 includes a centrifuge and an incubator 210. The incubator 210 is provided with micro-slats. The centrifuge is arranged inside the incubator 210. The centrifuge The machine is connected to the microplate strip. A plurality of microplate holes are provided on the microplate strip. The centrifuge is used to centrifuge the samples in the microplate holes. The incubator 210 is used to incubate the samples in the microplate holes. The incubator A first window 211 is opened at the upper end of 210; the sample adding mechanism 300 is arranged above the turntable mechanism 100. The sample adding mechanism 300 includes a first driving component and a sample adding needle. The first driving component is used to drive the sample adding needle to add the sample into the hole. In this way; the interpretation mechanism 400 is arranged above the turntable mechanism 100, and the interpretation mechanism 400 is used to interpret the experimental results of the sample.

Specifically, when performing an antibody detection experiment, the first carrier component can be placed on the turntable mechanism 100, and the samples and reagents required for the antibody detection experiment can be placed in different positions of the first carrier component to perform the antibody detection experiment; When performing a cross-matching experiment, a second carrier assembly can be placed on the turntable mechanism 100, and the samples and reagents required for the cross-matching experiment can be placed at different positions of the second carrier assembly to perform the cross-matching experiment. That is, the solution of this application can effectively be compatible with platelet antibody detection experiments and cross-matching experiments by arranging detachable first carrier components and second carrier components, so as to meet different platelet detection needs and reduce platelet detection costs.

Referring to Figures 2 to 4, in some embodiments of the present application, the turntable mechanism 100 is an annular hollow structure, the turntable mechanism 100 is provided with a first driving unit and a second driving unit 133, and the carrier assembly is provided outside the turntable mechanism 100. Circle, the first driving unit and the second driving unit 133 are both connected to the carrier assembly. The incubator 210 is disposed in the middle of the turntable mechanism 100. The first drive unit is used to drive the turntable mechanism 100 to drive the carrier assembly to rotate to a preset position. , the second driving unit 133 is used to drive the reagent bottle to rotate and mix. By arranging the turntable mechanism 100 as an annular hollow structure, placing the incubator 210 in the middle of the turntable mechanism 100 , and arranging the carrier assembly on the outer ring of the turntable mechanism 100 , space can be effectively utilized and the occupation of the centrifugal incubation mechanism 200 can be reduced. When a sample needs to be added, the first driving unit drives the outer ring of the turntable mechanism 100 to rotate and rotate the corresponding sample position to a preset position so that the sample can be processed accordingly. The second driving unit 133 is used to drive the reagent bottle loaded with the reagent to be mixed to rotate and mix.

Specifically, the turntable mechanism 100 is also provided with a carrier base 160 and a turntable base 150. A support column 190 is connected between the turntable base 150 and the carrier base 160. The turntable base 150 is connected to the first driving unit, and the first driving unit drives the turntable. The base 150 rotates, thereby driving the carrier base 160 and the carrier assembly to rotate along the center of the annular hollow structure. The first drive unit can be set as a stepper motor, and the system controls the rotation angle of the motor to control the carrier assembly to rotate to the preset position. to the sample adding position and perform subsequent sample adding actions.

Referring to FIG. 2 , in some embodiments of the present application, the first carrier assembly includes a reagent carrier 110 and a sample carrier 120 . The reagent carrier 110 and sample carrier 120 required for antibody detection are placed on the outer ring of the turntable mechanism 100, so that the sample carrier 120 and the reagent carrier 110 can be rotated to the bottom of the sample loading mechanism 300 respectively, and the sample can be transferred through the sample needle. and reagents are sequentially added to the centrifugal incubation mechanism 200, and the next step is performed.

Specifically, the first carrier assembly includes 4 sample carriers 120 and 1 reagent carrier 110. By arranging 4 sample carriers 120 and 1 reagent carrier 110, multiple samples can be placed at one time to avoid multiple samples. The action of placing samples on the outer ring of the turntable mechanism 100 can also complete the detection of multiple samples in each batch, thereby effectively improving detection efficiency.

Of course, the number of sample carriers 120 and reagent carriers 110 at this time is related to the specifications and models of the sample carriers 120 and reagent carriers 110 and the outer ring diameter of the turntable mechanism 100. In order to fully utilize the outer ring of the turntable mechanism 100 , the sample carrier 120 and the reagent carrier 110 can be spread all over the outer ring of the turntable mechanism 100 . Therefore, other numbers of sample carriers 120 and reagent carriers 110 can also be provided in conjunction with the turntable mechanism 100 .

Referring to FIG. 3 , in some embodiments of the present application, the second carrier assembly includes a sample carrier 120 , a reagent carrier 110 and a mixing carrier 130 , and the mixing carrier 130 is connected to the second driving unit 133 . The reagent carrier 110, sample carrier 120 and mixing carrier 130 required for cross-matching are placed on the outer ring of the turntable mechanism 100. The mixing carrier 130 is connected to the second driving unit 133, and the second driving unit 133 drives Mixing carrier 130 After the reagents placed therein are mixed, the mixed reagents and samples are moved to the centrifugal incubation mechanism 200 for the next step.

Specifically, the second carrier assembly includes a sample carrier 120 , a reagent carrier 110 , an eight-position mixing carrier 131 and two four-position mixing carriers 132 . By arranging one eight-position mixing carrier 131 and two four-position mixing carriers 132, multiple sets of reagents can be mixed at the same time, thereby improving the mixing efficiency and thereby improving the experimental efficiency of cross-matching.

Similarly, the specifications and quantities of the sample carrier 120 , the reagent carrier 110 and the mixing carrier 130 at this time are different from the specifications and models of the sample carrier 120 , the reagent carrier 110 and the mixing carrier 130 and the turntable mechanism 100 is related to the diameter of the outer ring. In order to fully utilize the outer ring of the turntable mechanism 100, the sample carrier 120, the reagent carrier 110 and the mixing carrier 130 can be placed all over the outer ring of the turntable mechanism 100. Therefore, other numbers of sample carriers 120 , reagent carriers 110 and mixing carriers 130 can also be provided in conjunction with the turntable mechanism 100 .

Referring to Figure 4, it can be understood that in order to facilitate the positioning of the carrier assembly on the turntable mechanism 100, a positioning column 170 and an anti-fool column 180 can be provided on the carrier base 160. The positioning column 170 is used for positioning the carrier assembly to prevent the positioning of the carrier assembly. The dead column 180 is used for position limiting to prevent the carrier assembly from being placed in the wrong position. Of course, in order to facilitate the taking and placing of the carrier assembly, a handheld portion 140 can be provided on the sample carrier 120 , the reagent carrier 110 and the mixing carrier 130 .

Referring to Figures 5 and 6, in some embodiments of the present application, the sample adding mechanism 300 is provided with two groups of sample adding channels. Each group of sample added channels is provided with at least one sample adding needle and one driving unit. The two groups of sample added The channels move independently in the vertical direction. By setting up two sets of independently ascending and descending loading channels, two sample positions can be loaded at the same time, thereby improving the loading efficiency and improving the experimental efficiency of platelet antibody detection and/or cross-matching.

Specifically, the two groups of sampling channels are respectively a first sampling channel 310 and a second sampling channel 320. The first sampling channel 310 is provided with a first sampling needle 311, and the second sampling channel 320 is provided with a first sampling needle 311. Two sampling needles 321, the first sampling needle 311 is driven by the third driving unit 312 to drive the corresponding synchronous wheel and timing belt, and the first screw 350 and the second sampling needle 321 are driven by the fourth driving unit 322 through the corresponding synchronization The wheel and the synchronous belt drive the screw to rotate. The third driving unit 312 and the fourth driving unit 322 can be motors. Of course, a first linear guide 330 can also be provided on the first sampling channel 310, and on the second sampling channel 330 can be provided. A second linear guide rail 327 is provided on 320 to respectively limit the movement of the first sample adding needle 311 and the second sample adding needle 321 in the vertical direction.

It is understandable that the sampling mechanism 300 can also be provided with three groups of sampling channels, or even more, but its structure and principle are similar to the foregoing contents, and are regarded as the essence of the sampling mechanism 300 with the two groups of sampling channels mentioned above. same. No further details will be given here.

Referring to Figure 6, in some embodiments of the present application, the interpretation mechanism 400 is connected to the sample adding mechanism. The interpretation mechanism 400 includes a fifth driving unit 328. The fifth driving unit 328 is used to drive the interpretation mechanism 400 to move in the vertical direction. By connecting the interpretation mechanism 400 with the sample adding mechanism 300, the interpretation mechanism 400 can be raised and lowered for interpretation while also saving the space occupied by the interpretation mechanism 400.

It can be understood that the interpretation mechanism 400 includes a camera 410 and a camera box 420 that protects the camera 410. A light-shielding sleeve 430 is connected to the lower end of the camera box 420. The first driving component drives the interpretation mechanism 400 through the first window 211 and descends to the incubator 210. When the upper end of the microplate hole is inside, the lower end of the light-shielding sleeve is in contact with the microplate hole, or is separated from the microplate hole by a first preset distance. At this time, when the camera 410 captures the experimental results in the microplate hole, it can almost completely isolate the outside. The interference of ambient light can effectively improve the accuracy of the interpretation of experimental results by the interpretation mechanism 400.

Further, the platelet detection device further includes a lever 360, which is connected to the fifth driving unit 328. The lever 360 is used to open or close the first window 211.

Further, a magnet 361 is provided at the lower end of the lever 360, and a blocking piece is provided at the upper end of the first window 211. The magnet 361 is used to cooperate with the blocking piece to open or close the first window 211.

Specifically, the fifth driving unit 328 is fixedly connected to the first fixing part 323, and the first fixing part 323 is fixedly connected to the second fixing part 324. The lever 360 is movably connected to the second fixing member 324 through the second adjustment hole provided. The second adjustment hole is a waist-shaped hole. The limiting nail 363 passes through the second adjustment hole provided on the lever 360 and is connected to the second fixing member 324 . 324 is fixedly connected, and the lever 360 is provided with a second connector 362. The second connector 362 is connected to the interpretation mechanism 400. As the interpretation mechanism 400 moves in the vertical direction, the amplitude of the rise or fall of the lever 360 is the second Adjust the length of the hole.

In addition, a first adapter 326 is provided at the lower end of the fifth drive unit 328. The first adapter 326 is fixedly connected to the shaft end of the fifth drive unit 328, and the first adapter 326 is fixedly connected to the third connecting member 325. A third fixing part 470 is fixedly provided on the third connecting part 325. The third fixing part 470 is connected to the camera pendant 440. The lower end of the camera pendant 440 is connected to the cover plate 450. The cover plate 450 is fixed to the camera 410. connect.

Of course, in order to ensure that the moving position of the interpretation mechanism 400 in the horizontal direction can be accurately aligned with the microplate hole at the lower end, and to ensure the descending distance of the interpretation mechanism 400, a sensing component 460 is provided on the interpretation mechanism 400, which can accurately sense the movement of the interpretation mechanism 400. The spatial position realizes the precise positioning of the interpretation mechanism 400 with a descending distance in the horizontal direction.

Specifically, the sensing component 460 includes a vertical direction sensing unit to accurately sense the position of the interpretation mechanism 400 in the vertical direction, thereby achieving precise positioning of the interpretation mechanism 400 in the vertical direction and improving the accuracy of interpretation.

The vertical direction sensing unit includes a positioning sensor 461 and a sensing piece 462. The positioning sensor 461 is arranged in a U-shape or a ring shape, so that the sensing piece 462 passes through the middle of the positioning sensor 461, and the positioning sensor 461 senses the sensing piece 462. The distance by which the sensing and interpretation mechanism 400 rises or falls through the distance.

Referring to Figure 7, in some embodiments of the present application, the platelet detection device further includes a plate washing mechanism 500. The plate washing mechanism 500 includes a plate washing head assembly 510 and a second driving assembly 520. The second driving assembly 520 is used to drive the plate washing. The head assembly 510 performs lifting and lowering movements. By arranging the plate washing head assembly 510, the liquid injection needle 511 and the liquid suction needle 512 on the plate washing head assembly 510 can be cleaned in time, which can save the consumable cost of the cleaning agent. While saving the cost, the platelet detection experiment can be effectively improved. accuracy.

Referring to Figures 9 and 10, further, the board washing head assembly 510 includes at least a set of liquid injection needles 511 and liquid suction needles 512. The liquid injection needle 511 and the liquid suction needle 512 are provided at the lower end of the board washing head assembly 510. The needle 511 and/or the liquid aspiration needle 512 is provided with an escape portion 513, and the escape portion 513 is bent toward the middle of the liquid injection needle 511 and the liquid aspiration needle 512. By arranging the liquid injection needle 511 and the liquid aspiration needle 512 as a group, liquid injection and liquid aspiration can be performed respectively in the same microplate well, thereby shortening the plate washing time. When the diameter of the microplate hole is small, if the injection needle 511 and the aspiration needle 512 are inserted into the same microplate hole at the same time, the phenomenon of needle collision is likely to occur, that is, the injection needle 511 or the aspiration needle 512 hits the microplate hole. The edges or inner walls may cause cleaning failure. Therefore, in this solution, the avoidance part 513 is provided at the preset position of the injection needle 511 and/or the suction needle 512, and the avoidance part 513 is bent toward the middle of the injection needle 511 and the suction needle 512, thereby effectively avoiding the impact of the needle. risks of.

Referring to FIG. 8 , further, a maintenance pool 560 is provided on one side of the second driving assembly 520 . The maintenance pool 560 is used for cleaning and maintenance of the washing head assembly 510 . By setting up a maintenance pool 560 for cleaning and maintaining the plate washer head assembly 510, the plate washer head assembly 510 can be cleaned and maintained in a timely manner to avoid cross-contamination of samples and at the same time improve the service life of the plate washer head assembly 510.

Referring to Figures 9 and 11, further, the washing mechanism 500 further includes a washing head hanger 580 and a washing head hanger 590. The upper end of the washing head assembly 510 is also provided with a first fixing hole 515 and a first adjustment hole 514. The washing head assembly 510 is fixedly connected to the washing head hanger 560 through the first fixing hole 515 and the first adjustment hole 514. The first adjustment hole 514 is used to adjust the angle of the washing head assembly 510 in the horizontal direction. By arranging the first adjustment hole 514 for adjusting the angle of the washing head assembly 510 in the horizontal direction, the position of the washing head assembly 510 in the horizontal direction can be accurately adjusted, thereby achieving precise positioning of the washing head assembly 510 and the microplate well, which can avoid The positioning is not accurate and the corresponding microplate hole cannot be inserted or there is a problem with the striker.

Specifically, the washer head hanger 580 is fixedly connected to the washer head assembly 510. The washer head hanger 590 is provided with a U-shaped groove 591. The U-shaped groove 591 is used to restrict the washer head hanger 580 from sliding in the vertical direction. There is one first fixing hole 515 and two first adjustment holes 514. The three holes are arranged in a triangular shape. The screws pass through the first fixing hole 515 of the washing head hanger 580 and are fixedly connected to the washing head assembly 510. Thereby, the washing head pendant 580 and the washing head assembly 510 are fixedly connected along the first fixing hole 515; the first adjustment hole 514 is set as a waist-shaped hole, which can adjust the rotation angle of the washing head assembly 510 in the horizontal direction, that is, with The first fixing hole 515 is used as the base point. Rotate and adjust the position of the washing head assembly 510. After the adjustment is in place, tighten the screw passing through the first adjustment hole 514 to fix the position of the washing head pendant 580 and the washing head assembly 510. , thereby enabling the washing head assembly 510 to be positioned more accurately. By designing adjustable waist-shaped holes, while achieving precise positioning, the processing accuracy of other matching parts can also be reduced, ultimately achieving the purpose of reducing costs.

Of course, the washing mechanism 500 also includes a bracket 570 , which is used to support the washing head assembly 510 and the maintenance tank 560 .

It can be understood that the second driving assembly 520 also includes a motor, a third linear guide 530, a second screw 540 and a hub 550. The motor drives the second screw 540 along the third straight line through the synchronous wheel and synchronous belt connected thereto. The guide rail 530 moves in the vertical direction, and the washing head hanger 590 is fixedly connected to the second driving assembly 520, thereby driving the washing head assembly 510 to move in the vertical direction.

In addition, in order to avoid multiple wire connections from being entangled with each other, a hub 550 is used to organize and collect them, which can not only increase the service life of the wire connections, but also improve the overall aesthetics of the platelet detection device.

The following is a detailed description of the use of the platelet detection device in this application using the procedures of the platelet antibody detection experiment and the platelet cross-matching experiment:

The experimental process of platelet antibody detection is:

The corresponding sample carrier 120 and the reagent carrier 110 are placed on the turntable mechanism 100, and the corresponding microplate strips are placed in the incubator 210; wherein, there are four sample carriers and microplate strips, and each sample carrier There are 8 holes on both the rack and the microstrip. The platelet suspension, indicator red blood cells, negative control, positive control, low ionic strength solution, and anti-human IgG solution are placed on the reagent carrier in sequence;

Place multiple test tubes loaded with different samples into the corresponding sample holes of the sample carrier 120, and place multiple reagent bottles loaded with different reagents into the corresponding reagent holes of the reagent carrier 110;

The reagent bottles containing red blood cells and platelets are connected to the second driving unit 133. The second driving unit 133 drives the reagent bottle containing platelets and the reagent bottle indicating red blood cells to rotate and mix them;

The fifth driving unit 328 drives the lever 360 downward, and cooperates with the stopper at the upper end of the first window 211. After opening the first window 211, the fifth driving unit 328 rises and returns to its original position;

Add 50 μL platelet suspension into each microplate well of the microplate strip;

Use a centrifuge to shake the platelet suspension and the coating in the microplate wells of the microstrip until they are fully in contact, and then centrifuge them for 5 minutes so that the platelet suspension forms a platelet monolayer at the bottom of the microplate wells of the microslats. ;

Then use the plate washing head assembly 510 to wash the microplate wells of the microplate strip to remove the supernatant in the upper layer including unbound platelets;

Add 100 μL of low ionic strength solution into the microplate well of each microplate strip;

Add 50 μL of sample and negative and positive controls into the microplate well of each microplate strip;

Use a centrifuge to shake the microplate wells of the microplate again;

The fifth driving unit 328 drives the lever 360 downward, cooperates with the baffle at the upper end of the first window 211, closes the first window 211 and rises back to the original position;

Use incubator 210 to incubate the microplate strips and microplate wells at 37°C for 30 minutes;

Then use the plate washing head assembly 510 to wash the microplate wells of the microplate strip, and suck away the cleaning solution and unbound samples;

Add 50 μL anti-human IgG solution into the microplate well of each microplate strip;

Add 50 μL of indicator red blood cells into the microplate well of each microplate; use a centrifuge to shake the microplate well and centrifuge; and

After centrifugal incubation, the fifth driving unit 328 drives the lever 360 again to open the incubator 210, and drives the interpretation mechanism 400 to descend a preset distance (the light-shielding sleeve 430 is in contact with the upper end of the microplate hole, or is separated by a preset distance), and the microplate is The experimental results in the wells are interpreted to obtain the platelet antibody detection results.

The experimental process of platelet cross-matching is:

The corresponding sample carrier 120, the reagent carrier 110 and the mixing carrier 130 are placed on the turntable mechanism 100, and the corresponding microslats are placed in the incubator 210; wherein, the anti-human IgG solution is placed on the reagent carrier in sequence. , positive control, negative control, low ionic strength solution; the indicator red blood cells are placed in the designated holes on the mixing carrier 130;

Place multiple reagent bottles containing platelet suspensions containing platelets from different donors on the mixing carrier 130;

Mix the test tubes containing the platelet suspension and the reagent bottles containing the indicator red blood cells placed on the mixing carrier 130 respectively;

Add 50 μL platelet suspension into the microplate well of each microplate strip;

Use a centrifuge to shake it evenly so that the platelet suspension fully contacts the coating in the microplate wells of the microslats, and then centrifuge for 5 minutes to allow the platelet suspension to form a platelet monolayer at the bottom of the microplate wells of the microslats. ;

Use the plate washing head assembly 510 to wash the microplate wells of the microplate strip to remove the supernatant including unbound platelets;

Use a centrifuge to shake the solution in the microplate wells of the microstrip until the sample is fully in contact with the platelet monolayer at the bottom of the microplate wells of the microstrip;

Use incubator 210 to incubate the solution in the microplate wells of the microplate at 37°C for 30 minutes;

Use the plate washing head assembly 510 to wash the microplate wells of the microplate strip to remove unbound samples;

Add 50 μL of indicated red blood cells into the microplate well of each microplate strip;

Use a centrifuge to shake and centrifuge the solution in the microplate wells of the microplate; and

The fifth driving unit 328 drives the lever 360 again to open the incubator 210, and drives the interpretation mechanism 400 to descend a preset distance (the light-shielding sleeve 430 is in contact with the upper end of the microplate hole, or is separated by a preset distance), and the experiment in the microplate hole is The results are interpreted and the platelet antibody test results are obtained.

The embodiments of the present application have been described in detail above in conjunction with the accompanying drawings. However, the present application is not limited to the above-mentioned embodiments. Various changes can be made within the knowledge scope of those of ordinary skill in the technical field without departing from the purpose of the present application. .

In the description of this specification, reference to the terms "one embodiment," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" or the like is intended to be incorporated into the description of the implementation. An example or example describes a specific feature, structure, material, or characteristic that is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The applicant declares that the above-described embodiments only express the basic principles, main features and advantages of the present application. Those skilled in the industry should understand that the present application is not limited by the above-mentioned embodiments. What is described in the above-mentioned embodiments and descriptions is only the principle of the present application. For those of ordinary skill in the industry, the application can be made without departing from the concept and scope of the present application. Under the premise, various changes and improvements can also be made, and these changes and improvements all fall within the scope of the claimed application.

Although the embodiments of the present application have been shown and described, those of ordinary skill in the art will understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and purposes of the present application. , the scope of the application is defined by the claims and their equivalents.

Claims

A platelet detection device, including:

Turntable mechanism, the turntable mechanism is detachably provided with a carrier assembly, the carrier assembly includes a first carrier assembly or a second carrier assembly, the carrier assembly is provided with a test tube hole, the test tube hole Used to place reagent bottles loaded with samples or corresponding types of reagents; wherein the first carrier component is used to perform antibody detection experiments, and the second carrier component is used to perform cross-matching experiments;

Centrifugal incubation mechanism, the centrifugal incubation mechanism is arranged on one side of the turntable mechanism, the centrifugal incubation mechanism includes a centrifuge and an incubator, the incubator is provided with micro-slats, the centrifuge is arranged on the Inside the incubator, the centrifuge is connected to the microplate strip, and a plurality of microplate holes are provided on the microplate strip. The centrifuge is used to centrifuge the sample in the microplate hole. The incubator is used to incubate the samples in the microplate wells, and a first window is provided at the upper end of the incubator;

A sample adding mechanism is provided above the turntable mechanism. The sample adding mechanism includes a first driving component and a sample adding needle. The first driving component is used to drive the sample adding needle toward the Add sample into the well; and

An interpretation mechanism is provided above the turntable mechanism, and is used to interpret the experimental results of the sample.
The platelet detection device according to claim 1, wherein the turntable mechanism is an annular hollow structure, the turntable mechanism is provided with a first drive unit and a second drive unit, and the carrier assembly is disposed on the outer ring of the turntable mechanism. On the machine, the first driving unit and the second driving unit are both connected to the carrier assembly, the incubator is arranged in the middle of the turntable mechanism, and the first drive unit is used to drive the turntable mechanism. The carrier assembly rotates to a preset position, and the second driving unit is used to drive the reagent bottle to rotate and shake evenly.
The platelet detection device according to claim 2, wherein the first carrier assembly includes a reagent carrier and a sample carrier.
The platelet detection device according to claim 3, wherein the first carrier assembly includes four sample carriers and one reagent carrier.
The platelet detection device according to any one of claims 2 to 4, wherein the second carrier assembly includes a sample carrier, a reagent carrier and a mixing carrier, and the mixing carrier and the second driving unit connect.
The platelet detection device according to claim 5, wherein the second carrier assembly includes a sample carrier, a reagent carrier, an eight-position mixing carrier and two four-position mixing carriers. .
The platelet detection device according to any one of claims 1 to 6, wherein the sampling mechanism is provided with two groups of sampling channels, each group of sampling channels is provided with at least one sampling needle and one driving unit, two The group of sample adding channels can move up and down independently in the vertical direction.
The platelet detection device according to any one of claims 1 to 7, wherein the interpretation mechanism is connected to the sample adding mechanism, the interpretation mechanism includes a fifth driving unit, and the fifth driving unit is used to drive the The interpretation mechanism moves in the vertical direction.
The platelet detection device according to claim 8, further comprising a lever connected to a fifth driving unit, and the lever is used to open or close the first window.
The platelet detection device according to claim 9, wherein a magnet is provided at the lower end of the lever, and a blocking piece is provided at the upper end of the first window, and the magnet is used to cooperate with the blocking piece to open or close the first window. .
The platelet detection device according to any one of claims 8 to 10, wherein the reading mechanism is further provided with a sensing component, and the sensing component is used to sense the spatial position of the reading mechanism.
The platelet detection device according to any one of claims 8 to 11, wherein the interpretation mechanism further includes a camera, a camera box and a light-shielding sleeve, the camera is disposed in the middle of the camera box, and the light-shielding sleeve is disposed at the lower end of the camera box.
The platelet detection device according to any one of claims 1 to 12, further comprising a plate washing mechanism, the plate washing mechanism includes a plate washing head assembly and a second driving assembly, the second driving assembly is used to drive the washing plate. The board head assembly performs lifting and lowering movements.
The platelet detection device according to claim 13, wherein the washing head assembly includes at least a set of injection needles and aspiration needles, and the injection needle and the absorption needle are arranged on the plate washing head assembly. At the lower end of the liquid injection needle and/or the liquid suction needle, an escape portion is provided, and the escape portion is bent toward the middle of the liquid injection needle and the liquid suction needle.
The platelet detection device according to claim 13 or 14, wherein a maintenance tank is further provided on one side of the second driving component, and the maintenance tank is used for cleaning and maintenance of the plate washing head assembly.
The platelet detection device according to any one of claims 13 to 15, wherein the plate washing mechanism further includes a plate washing head hanger and a plate washing head hanger, and the upper end of the plate washing head assembly is also provided with a first fixing hole. and a first adjustment hole. The washing head assembly is fixedly connected to the washing head hanger through the first fixing hole and the first adjustment hole. The first adjustment hole is used to adjust the washing head. The angle of the component in the horizontal direction.