CN110749743A - Washing device for chemiluminescence immunity analyzer - Google Patents
Washing device for chemiluminescence immunity analyzer Download PDFInfo
- Publication number
- CN110749743A CN110749743A CN201911209322.6A CN201911209322A CN110749743A CN 110749743 A CN110749743 A CN 110749743A CN 201911209322 A CN201911209322 A CN 201911209322A CN 110749743 A CN110749743 A CN 110749743A
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- magnet
- magnet mounting
- needle
- adsorption
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
Abstract
The invention relates to the technical field of production and manufacture of chemiluminescence immunoassay analyzers, in particular to a washing device for a chemiluminescence immunoassay analyzer, which effectively improves the washing effect on the premise of not adding washing times, and is characterized in that a magnetic bead adsorption mechanism is provided with a magnet mounting plate, the magnet mounting plate is provided with more than two magnet mounting grooves, two magnets are arranged in each magnet mounting groove side by side up and down, the magnetic pole directions of the two magnets positioned in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets positioned at the same height in the adjacent magnet mounting grooves are also reversed; the magnet mounting panel or the rotary disk inside wall corresponding to the adsorption treatment initial position department still are equipped with the adsorption magnet mounting groove in advance, and a magnet is installed in the adsorption magnet mounting groove in advance.
Description
The technical field is as follows:
the invention relates to the technical field of production and manufacture of chemiluminescence immunoassay analyzers, in particular to a washing device for a chemiluminescence immunoassay analyzer, which can effectively improve the washing effect on the premise of not adding washing times.
Background art:
a full-automatic chemiluminescence immunoassay analyzer is an ultramicro analysis device established by combining immunoreaction and luminescence analysis, for example, a full-automatic chemiluminescence immunoassay analyzer cleaning device disclosed in patent document CN103599898 provides a cleaning structure for the streamline cleaning of sample magnetic beads, but in the actual use process, the following defects exist in the existing products: (1) the lower needle position of the cleaning needle is positioned at the center of the reaction cup, so that a small amount of magnetic beads carrying effective substances can be sucked away when waste liquid is sucked, the magnetic beads are lost, and the cross contamination risk exists; (2) the magnetic bead adsorption equipment that its adopted adopts single magnet structure, and suction is weak, and consequently the magnetic bead adsorbs slowly, and single magnet knot constructs into the magnetic bead and adsorbs and be some or strip gathering, and consequently the magnetic bead forms many intermediate layers adsorption structure easily, leads to the unable clearance of impurity in the adjacent intermediate layer.
The invention content is as follows:
aiming at the defects and shortcomings in the prior art, the invention provides the washing device for the chemical immunoassay method optical analyzer, which can effectively improve the washing effect on the premise of not adding washing times.
The invention can be achieved by the following measures:
a washing device for a chemical immunity method optical analyzer is provided with a magnetic bead adsorption mechanism, and is characterized in that the magnetic bead adsorption mechanism is provided with a magnet mounting plate, the magnet mounting plate is provided with more than two magnet mounting grooves, two magnets are arranged in each magnet mounting groove side by side from top to bottom, the magnetic pole directions of the two magnets positioned in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets positioned at the same height in the adjacent magnet mounting grooves are also reversed; the magnet mounting panel or the rotary disk inside wall corresponding to the adsorption treatment initial position department still are equipped with the adsorption magnet mounting groove in advance, and a magnet is installed in the adsorption magnet mounting groove in advance.
According to the invention, two pre-adsorption magnet mounting grooves are arranged in parallel at the position, corresponding to the adsorption treatment starting position, on the magnet mounting plate along the rotating advancing direction of the rotating disc of the reaction cup, and a magnet is arranged in each pre-adsorption magnet mounting groove respectively and used for performing pre-adsorption treatment on magnetic beads in the reaction cup before the washing process starts, so that the adsorption cleaning effect is improved.
The invention is also provided with a reaction cup rotating disc and a lifting needle frame, wherein the reaction cup rotating disc is provided with a channel for passing a sample, the magnetic bead adsorption mechanism is arranged on the side surface of the channel, and the lifting support is arranged above the channel.
The lifting needle frame is provided with at least two groups of liquid injection and absorption combined needles, each liquid injection and absorption combined needle consists of a liquid suction needle and a liquid injection needle, the needle point position of the lower end of the liquid suction needle is lower than the needle point position of the lower end of the liquid injection needle, and the needle point of the liquid injection needle is bent towards the direction close to the liquid suction needle, so that when waste liquid is sucked, the needle point of the liquid suction needle can reach the bottom of a sample in a reaction cup to fully suck impurities, when cleaning liquid is injected, the needle point of the liquid injection needle is positioned above the needle point of the liquid suction needle, and the cleaning liquid injected by the liquid injection needle can flush the liquid suction needle, so that different samples are prevented from being.
The liquid suction needle and the liquid injection needle are arranged on one side, which is deviated from the magnet adsorption mechanism, above the reaction cup through the lifting support, so that the liquid suction needle is ensured to descend to the opposite side, which is adsorbed and agglomerated, of magnetic beads in the reaction cup when sucking liquid, and the magnetic beads are prevented from losing.
The magnet mounting plate comprises a vertical plate and a bottom plate, wherein the vertical plate and the bottom plate are vertically and fixedly connected, the cross section of the vertical plate is L-shaped, more than two U-shaped grooves for placing magnets are formed in the side surface of the vertical plate, the opening direction of the U-shaped grooves is upward, a pressing plate for closing the openings of the more than two U-shaped grooves is arranged at the top of the vertical plate, the pressing plate is fixedly connected with the top of the vertical plate through screws, and the bottom plate is provided with splicing teeth for being embedded with a rotating; two magnets which are arranged side by side up and down are arranged in a U-shaped groove on the side face of the vertical plate, and an antirust iron plate is arranged on the outer side of each magnet and used for strengthening magnetism; the vertical plate and the bottom plate are both in an arc shape which is matched with the inner side of the rotating disk main body, the rotating disk is provided with a reaction cup carrying track, the corresponding position of the washing position on the inner wall is provided with an installation notch which is used for assembling the magnet installation plate and communicated with the reaction cup carrying track, and the installation notch is provided with an installation groove matched with the assembling teeth.
In the using process of the invention, the reaction cup rotating disc drives more than two reaction cups containing samples to be tested to move forward according to a set direction, the driving mechanism for driving the reaction cup rotating disc to rotate can drive the reaction cup rotating disc to move forward one reaction cup position each time, when the washing treatment starts, the reaction cup firstly enters the pre-adsorption treatment area under the driving of the rotating disc, single-particle magnets in the pre-adsorption magnet installation grooves on the magnet installation plate carry out pre-adsorption treatment on magnetic beads in the reaction cup, so that the magnetic beads originally scattered in the whole space of the reaction cup are close to one side of the magnetic bead adsorption mechanism, when the rotating disc drives the reaction cup to enter the double-particle magnet adsorption area, the magnetic beads are quickly adsorbed to the cup wall at one side of the reaction cup close to the magnet, at the moment, because double-particle magnets are adopted for adsorption, the magnetic beads in the reaction cup are flatly agglomerated on the wall of, the influence of impurities wrapped by multi-layer agglomerated magnetic beads on the cleaning effect is avoided; meanwhile, the lifting needle frame is driven by the needle frame driving mechanism to lift to complete the injection of the cleaning liquid and the absorption of the waste liquid, wherein the needle point position of the lower end of the liquid suction needle is lower than the needle point position of the lower end of the liquid injection needle, and the needle point of the liquid injection needle is bent towards the direction close to the liquid suction needle, so that when the waste liquid is absorbed, the needle point of the liquid suction needle can reach the bottom of the sample in the reaction cup to fully absorb impurities, when the cleaning liquid is injected, the needle point of the liquid injection needle is positioned above the needle point of the liquid suction needle, and the cleaning liquid injected by the liquid injection needle can flush the liquid suction needle, so that different; in addition, the liquid suction needle and the liquid injection needle are arranged on one side of the reaction cup above the magnet adsorption mechanism through the lifting support, so that the liquid suction needle is ensured to descend to the opposite side of the reaction cup where magnetic beads are adsorbed and agglomerated when sucking liquid, and the magnetic beads are prevented from losing.
Compared with the prior art, the invention can effectively improve the cleaning effect and reduce the loss of magnetic beads on the premise of not increasing the cleaning times, and has the obvious advantages of reasonable structure, simple and convenient operation, reliable work and the like.
Description of the drawings:
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a structural schematic diagram of the liquid injection and absorption combined needle in the invention.
Fig. 3 is a sectional view taken along the line a-a in fig. 2.
Fig. 4 is a schematic structural view of a magnet mounting plate according to the present invention.
Reference numerals: the device comprises a rotating disk 1, a lifting needle frame 2, a magnet 3, a pre-adsorption magnet mounting groove 4, a magnet mounting plate 5, a liquid injection and absorption combined needle 6, a liquid absorption needle 7, a liquid injection needle 8, a vertical plate 9, a bottom plate 10, a magnetic bead adsorption surface 11, a U-shaped groove 12, a pressing plate 13 and splicing teeth 14.
The specific implementation mode is as follows:
the invention will be further described with reference to the accompanying drawings.
As shown in the attached figure 1, the invention provides a washing device for a chemical immunoassay method optical analyzer, which is provided with a reaction cup rotating disc 1, a lifting needle frame 2 and a magnetic bead adsorption mechanism and is characterized in that the magnetic bead adsorption mechanism is provided with a magnet mounting plate 5, the magnet mounting plate 5 is provided with more than two magnet mounting grooves, two magnets 3 are arranged in each magnet mounting groove in parallel up and down, the magnetic pole directions of the two magnets 3 positioned in the same magnet mounting groove are reversed, and the magnetic attraction directions of the two magnets 3 positioned at the same height in the adjacent magnet mounting grooves are also reversed; the magnet mounting panel 5 or correspond on the 1 inside wall of rotary disk and adsorb initial position department and still be equipped with in advance and adsorb magnet mounting groove 4, magnet of installation in the magnet mounting groove 4 in advance.
According to the invention, two pre-adsorption magnet mounting grooves 4 are arranged in parallel at the position, corresponding to the adsorption treatment starting position, on the magnet mounting plate 5 along the rotating advancing direction of the rotating disc of the reaction cup, and each pre-adsorption magnet mounting groove 4 is internally provided with a magnet respectively, so that the pre-adsorption treatment is carried out on magnetic beads in the reaction cup before the washing process is started, and the adsorption cleaning effect is improved.
As shown in the attached drawing 2, at least two groups of liquid injection and absorption combined needles 6 are arranged on the lifting needle frame 2, each liquid injection and absorption combined needle 6 comprises a liquid suction needle 7 and a liquid injection needle 8, wherein the needle point position of the lower end of the liquid suction needle 7 is lower than the needle point position of the lower end of the liquid injection needle 8, and the needle point of the liquid injection needle 8 is bent towards the direction close to the liquid suction needle 7, so that when waste liquid is sucked, the needle point of the liquid suction needle 7 can reach the bottom of a sample in the reaction cup 9, impurities are fully sucked away, when cleaning liquid is injected, the needle point of the liquid injection needle 8 is positioned above the needle point of the liquid suction needle 7, and cleaning liquid injected by the liquid injection needle 8 can flush the needle point of the liquid suction needle 7.
As shown in the attached figure 3, the liquid suction needle 7 and the liquid injection needle are both arranged on one side of the reaction cup deviating from the magnet adsorption mechanism through the lifting support 2, so that the liquid suction needle 7 is ensured to descend to the opposite side of the reaction cup where magnetic beads are adsorbed and agglomerated when sucking liquid, and the magnetic beads are prevented from losing.
As shown in fig. 4, the magnet mounting plate 5 of the present invention is composed of a vertical plate 9 and a bottom plate 10, the vertical plate 9 and the bottom plate 10 are vertically and fixedly connected, and the cross section is L-shaped, the side surface of the vertical plate 9 is provided with more than two U-shaped grooves 12 for placing magnets, the opening direction of the U-shaped grooves 12 is upward, the top of the vertical plate 9 is provided with a pressing plate 13 for closing the openings of the more than two U-shaped grooves 12, the pressing plate 13 is fixedly connected with the top of the vertical plate 9 through screws, and the bottom plate 10 is provided with a jogged tooth 14 for; two magnets which are arranged side by side up and down are arranged in a U-shaped groove on the side face of the vertical plate 9, an antirust iron plate is arranged on the outer side of each magnet and used for strengthening magnetism, and furthermore, a shading adhesive tape is pasted on the outer side of the antirust iron plate so as to improve the light shading performance of the whole mechanism; the vertical plate 9 and the bottom plate 10 are both in an arc shape which is matched with the inner side of the main body of the rotating disk 1, the vertical plate 9 and the bottom plate 10 are in an integrated structure, a reaction cup carrying track is arranged on the rotating disk 1, a mounting notch which is used for assembling the magnet mounting plate and is communicated with the reaction cup carrying track is arranged at the corresponding position of the washing position on the inner wall, and a mounting groove matched with the assembling teeth 14 is arranged on the mounting notch.
In the using process of the invention, the reaction cup rotating disc drives more than two reaction cups containing samples to be tested to move forward according to a set direction, the driving mechanism for driving the reaction cup rotating disc to rotate can drive the reaction cup rotating disc to move forward one reaction cup position each time, when the washing treatment starts, the reaction cup firstly enters the pre-adsorption treatment area under the driving of the rotating disc, single-particle magnets in the pre-adsorption magnet installation grooves on the magnet installation plate carry out pre-adsorption treatment on magnetic beads in the reaction cup, so that the magnetic beads originally scattered in the whole space of the reaction cup are close to one side of the magnetic bead adsorption mechanism, when the rotating disc drives the reaction cup to enter the double-particle magnet adsorption area, the magnetic beads are quickly adsorbed to the cup wall at one side of the reaction cup close to the magnet, at the moment, because double-particle magnets are adopted for adsorption, the magnetic beads in the reaction cup are flatly agglomerated on the wall of, the influence of impurities wrapped by multi-layer agglomerated magnetic beads on the cleaning effect is avoided; meanwhile, the lifting needle frame is driven by the needle frame driving mechanism to lift to complete the injection of the cleaning liquid and the absorption of the waste liquid, wherein the needle point position of the lower end of the liquid suction needle is lower than the needle point position of the lower end of the liquid injection needle, and the needle point of the liquid injection needle is bent towards the direction close to the liquid suction needle, so that when the waste liquid is absorbed, the needle point of the liquid suction needle can reach the bottom of the sample in the reaction cup to fully absorb impurities, when the cleaning liquid is injected, the needle point of the liquid injection needle is positioned above the needle point of the liquid suction needle, and the cleaning liquid injected by the liquid injection needle can flush the liquid suction needle, so that different; in addition, the liquid suction needle and the liquid injection needle are arranged on one side of the reaction cup above the magnet adsorption mechanism through the lifting support, so that the liquid suction needle is ensured to descend to the opposite side of the reaction cup where magnetic beads are adsorbed and agglomerated when sucking liquid, and the magnetic beads are prevented from losing.
Compared with the prior art, the invention can effectively improve the cleaning effect and reduce the loss of magnetic beads on the premise of not increasing the cleaning times, and has the obvious advantages of reasonable structure, simple and convenient operation, reliable work and the like.
Claims (7)
1. The utility model provides a chemistry immunity method is washing device for optical analysis appearance, is equipped with magnetic bead adsorption apparatus structure, its characterized in that magnetic bead adsorption apparatus structure is equipped with the magnet mounting panel, sets up two above magnet mounting grooves on the magnet mounting panel, sets up two magnet from top to bottom side by side in every magnet mounting groove, and the magnetic pole direction of two magnet that are located same magnet mounting groove is put conversely, and the magnetism that is located two magnet of same height in adjacent magnet mounting groove is inhaled the direction and is put conversely also.
2. The washing device for a chemical immunoassay optical analyzer according to claim 1, wherein a pre-attracting magnet installation groove is further provided on the inner side wall of the magnet installation plate or the rotary disk corresponding to the initial position of the adsorption treatment, and a magnet is installed in the pre-attracting magnet installation groove.
3. The washing device for the chemical immunoassay optical analyzer according to claim 1, wherein two pre-adsorption magnet mounting grooves are provided in parallel at positions on the magnet mounting plate corresponding to the start positions of the adsorption treatment along the forward direction of rotation of the rotating disk of the cuvette, and each pre-adsorption magnet mounting groove is provided with a magnet for pre-adsorbing beads in the cuvette before the washing process starts, thereby improving the adsorption cleaning effect.
4. The washing device for the chemical immunoassay optical analyzer as defined in claim 1, further comprising a rotating disk of the cuvette, a lifting needle holder, wherein the rotating disk of the cuvette is provided with a passage for passing the sample, the magnetic bead adsorption mechanism is disposed at a side of the passage, and the lifting holder is disposed above the passage.
5. The washing device for a chemoimmunoassay optical analyzer according to claim 1, wherein the lifting needle frame is provided with at least two sets of liquid injection and absorption combined needles, each of which is composed of a liquid absorption needle and a liquid injection needle, wherein the lower end of the liquid absorption needle is lower than the lower end of the liquid injection needle, and the tip of the liquid injection needle is bent in a direction close to the liquid absorption needle.
6. The washing device for the chemiluminescence immunoassay optical analyzer according to claim 5, wherein the liquid suction needle and the liquid injection needle are both arranged on the side of the reaction cup deviating from the magnet adsorption mechanism through the lifting bracket.
7. The washing device for the chemical immunoassay optical analyzer according to claim 1, wherein the magnet mounting plate is composed of a vertical plate and a bottom plate, the vertical plate and the bottom plate are vertically and fixedly connected, the cross section of the vertical plate is in an L shape, more than two U-shaped grooves for placing magnets are arranged on the side surface of the vertical plate, the opening direction of the U-shaped grooves is upward, a pressing plate for closing the openings of the more than two U-shaped grooves is arranged on the top of the vertical plate, the pressing plate is fixedly connected with the top of the vertical plate through screws, and the bottom plate is provided with splicing teeth for being embedded with the rotary chassis; two magnets which are arranged side by side up and down are arranged in a U-shaped groove on the side face of the vertical plate, an antirust iron plate is arranged on the outer side of each magnet and used for strengthening magnetism, and a shading adhesive tape is pasted on the outer side of each antirust iron plate so as to improve the light shading performance of the whole mechanism; the vertical plate and the bottom plate are both in an arc shape which is matched with the inner side of the rotating disk main body, the rotating disk is provided with a reaction cup carrying track, the corresponding position of the washing position on the inner wall is provided with an installation notch which is used for assembling the magnet installation plate and communicated with the reaction cup carrying track, and the installation notch is provided with an installation groove matched with the assembling teeth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911209322.6A CN110749743A (en) | 2019-11-30 | 2019-11-30 | Washing device for chemiluminescence immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911209322.6A CN110749743A (en) | 2019-11-30 | 2019-11-30 | Washing device for chemiluminescence immunity analyzer |
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| Publication Number | Publication Date |
|---|---|
| CN110749743A true CN110749743A (en) | 2020-02-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911209322.6A Pending CN110749743A (en) | 2019-11-30 | 2019-11-30 | Washing device for chemiluminescence immunity analyzer |
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| CN (1) | CN110749743A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113770104A (en) * | 2021-10-20 | 2021-12-10 | 深圳市博辰智控有限公司 | Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer |
| CN113787070A (en) * | 2021-11-17 | 2021-12-14 | 南京万承生物科技有限公司 | Dynamic cleaning station of chemiluminescence immunoassay analyzer |
| WO2024040922A1 (en) * | 2022-08-24 | 2024-02-29 | 深圳市爱康生物科技股份有限公司 | Platelet testing device |
-
2019
- 2019-11-30 CN CN201911209322.6A patent/CN110749743A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113770104A (en) * | 2021-10-20 | 2021-12-10 | 深圳市博辰智控有限公司 | Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer |
| CN113787070A (en) * | 2021-11-17 | 2021-12-14 | 南京万承生物科技有限公司 | Dynamic cleaning station of chemiluminescence immunoassay analyzer |
| WO2024040922A1 (en) * | 2022-08-24 | 2024-02-29 | 深圳市爱康生物科技股份有限公司 | Platelet testing device |
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