CN214391372U

CN214391372U - ELISA plate cleaning device

Info

Publication number: CN214391372U
Application number: CN202120285682.0U
Authority: CN
Inventors: 贾思莹
Original assignee: Beijing Yuanjite Biotechnology Co ltd
Current assignee: Beijing Yuanjite Biotechnology Co ltd
Priority date: 2021-02-01
Filing date: 2021-02-01
Publication date: 2021-10-15
Anticipated expiration: 2031-02-01

Abstract

The utility model relates to an ELIAS plate belt cleaning device, including the host computer that is provided with control panel, be provided with on the host computer with the host computer complex mounting panel that transversely slides, the top that lies in the mounting panel on the host computer is provided with the complex washing head that vertically slides with the host computer, lay a plurality of liquid needles that are the matrix arrangement in the washing head towards the one side of mounting panel, one side and the liquid needle one-to-one that go out the liquid needle are provided with the imbibition needle, be provided with the liquid bottle that is used for holding the washing liquid and communicates with liquid needle and be used for holding the waste liquid and with the waste liquid bottle of imbibition needle intercommunication outside the host computer, be provided with in the host computer and be used for providing the power for the washing liquid and provide the peristaltic pump of vacuum for the imbibition needle. This application has the advantage that improves the abluent convenience of ELIAS plate, reduces staff's intensity of labour.

Description

ELISA plate cleaning device

Technical Field

The application relates to the field of cleaning equipment for biological agent detection, in particular to an ELISA plate cleaning device.

Background

At present, enzyme-linked immunosorbent assay is the most widely applied technique in enzyme immunoassay technology, and the main principle is that known antigen or antibody is adsorbed on the surface of a solid phase carrier, then enzyme-labeled antigen antibody is made to react on the surface of the solid phase carrier, then an antigen-antibody complex formed on the solid phase carrier is separated from other substances through washing, after an enzyme reaction substrate is added, the substrate and enzyme are subjected to chromogenic reaction, and qualitative or quantitative analysis can be carried out on the antibody or the antigen through the shade of the color; the ELISA plate is the most commonly used solid phase carrier for enzyme-linked reaction adsorption test and has very important function in enzyme immunoassay. The ELISA plate generally comprises a substrate, a plurality of hole sites which are arranged in a matrix are arranged on the substrate, enzyme-linked reaction is carried out in the hole sites, and after the ELISA plate is used, a washing device of the ELISA plate is needed to wash some uncombined substances and impurities after reaction in the hole sites, so that errors caused by residues in subsequent detection are reduced.

In the related technology, when a worker cleans an elisa plate, the washing liquid is mainly sucked by using a liquid-transferring gun and then injected into hole sites one by one, after the washing liquid is soaked for a period of time, the washing liquid in the hole sites is sucked out one by using the liquid-transferring gun, and then the washing liquid is repeatedly cleaned for multiple times, so that the impurities in the hole sites are ensured to be cleaned completely.

With respect to the related art in the above, the inventors consider that: the hole sites on the ELISA plate are more, and workers need to use a liquid-transferring gun to repeatedly clean the hole sites on the ELISA plate when large-scale tests are carried out, so that the labor intensity is relatively high; in addition, in order to reduce the possibility of cross contamination, the operator is required to frequently replace the pipette tip on the pipette, and the washing of the microplate is inconvenient.

SUMMERY OF THE UTILITY MODEL

In order to improve the abluent convenience of ELIAS plate, reduce staff's intensity of labour, this application provides an ELIAS plate belt cleaning device.

The application provides an ELIAS plate belt cleaning device adopts following technical scheme:

the utility model provides an ELIAS plate belt cleaning device, including the host computer that is provided with control panel, be provided with on the host computer with the host computer complex mounting panel that transversely slides, the top that lies in the mounting panel on the host computer is provided with the complex washing head that vertically slides with the host computer, lay a plurality of liquid needles that are the matrix arrangement in the washing head towards the one side of mounting panel, one side and the liquid needle one-to-one that go out the liquid needle are provided with the imbibition needle, be provided with the liquid bottle that is used for holding the washing liquid and communicates with liquid needle and be used for holding the waste liquid bottle that just communicates with the imbibition needle and be used for holding the waste liquid and communicate with the imbibition needle outside the host computer, be provided with in the host computer and be used for providing the power for the washing liquid and provide the peristaltic pump of vacuum for the imbibition needle.

By adopting the technical scheme, when a worker cleans an enzyme label plate, the enzyme label plate is firstly placed on the mounting plate, then the mounting plate is made to slide so that a hole position on the enzyme label plate corresponds to a liquid outlet needle and a liquid suction needle on the washing head, then the washing head is made to slide downwards, the liquid outlet needle is close to the hole position, the liquid suction needle is inserted into the bottom of the hole position, a peristaltic pump corresponding to the liquid outlet needle is started, washing liquid is pressed into the liquid outlet needle from a washing liquid bottle and flows into the hole position from the liquid outlet needle under the action of the peristaltic pump, after the washing liquid is soaked for a period of time, the peristaltic pump corresponding to the liquid suction needle is started, and the washing liquid in the hole position is sucked away and stored into a waste liquid bottle under the action of the peristaltic pump; and then repeating the operation until all impurities in the hole sites are cleaned, thereby improving the convenience of cleaning the ELISA plate and reducing the labor intensity of workers.

Optionally, a driving assembly for driving the mounting plate to slide is arranged in the main frame; a sliding groove is formed in the host along the sliding direction of the mounting plate, the driving assembly comprises a driving lead screw, a driving nut and a driving motor, the driving lead screw is arranged in the sliding groove and is in rotating fit with the sliding groove, the driving nut is matched with the driving lead screw, and the driving motor is used for pushing the driving lead screw to rotate; the mounting plate is provided with a sliding block, the sliding block is embedded in the sliding groove and is in sliding fit with the sliding groove, and the sliding block is fixedly connected with the driving nut.

By adopting the technical scheme, when cleaning the ELISA plate, a worker places the ELISA plate on the mounting plate, then starts the driving motor, the driving motor drives the driving screw rod to rotate, the driving screw rod drives the driving nut to slide, and the driving nut drives the mounting plate to slide, so that the hole position of the ELISA plate corresponds to the washing head; through setting up drive assembly, further improve the abluent convenience of ELIAS plate, reduce staff's intensity of labour.

Optionally, a lifting assembly for driving the washing head to slide up and down is arranged in the main machine; the washing machine is characterized in that a mounting groove is formed in the main machine along the sliding direction of the washing head, the lifting assembly comprises a lifting screw rod, a lifting nut and a lifting motor, the lifting screw rod is arranged in the mounting groove and is in running fit with the main machine, the lifting nut is matched with the lifting screw rod, the lifting motor is used for driving the lifting screw rod to rotate, and the washing head is connected with the lifting nut.

By adopting the technical scheme, when the driving assembly is started to enable the mounting plate to drive the ELISA plate to slide to the hole position corresponding to the washing head, the lifting motor is started to drive the lifting screw rod to rotate, and the lifting screw rod drives the lifting nut to slide up and down, so that the washing head is lifted up and down, and the liquid suction needle is conveniently inserted into the hole position of the ELISA plate; through setting up lifting unit, further improve the abluent convenience of ELIAS plate, reduce staff's intensity of labour.

Optionally, the sliding grooves are symmetrically formed in two sides of the washing head, and the two driving screw rods are respectively arranged in the sliding grooves.

Through adopting above-mentioned technical scheme, set up the spout at the avris of washing head, can reduce when washing liquid spills and pass the spout and cause the possibility of polluting to the drive screw, improve the stability of mounting panel when sliding simultaneously.

Optionally, two ends of the driving screw rod facing the same direction are respectively and coaxially and fixedly connected with a worm wheel, the output end of the driving motor is coaxially and fixedly connected with a worm, and the worm is meshed with the worm wheels on the two driving screw rods.

By adopting the technical scheme, the driving motor is started, the driving motor drives the worm to rotate, and the worm drives the two worm gears to synchronously rotate, so that the two driving lead screws synchronously rotate; through setting up worm wheel and worm, improve the stability that the installation piece slided.

Optionally, a caulking groove matched with the elisa plate is formed in the mounting plate.

By adopting the technical scheme, the stability of the enzyme label plate in the sliding process of the mounting plate is improved.

Optionally, a clamping piece for clamping and fixing the elisa plate is arranged on the mounting plate.

By adopting the technical scheme, the stability of the ELISA plate is further improved in the sliding process of the mounting plate and the cleaning of the hole sites of the ELISA plate.

Optionally, a mounting hole is formed in the inner wall of the caulking groove; the clamping piece comprises a clamping head which is arranged in the mounting hole and is matched with the mounting hole in a sliding way and a spring which is arranged in the mounting hole and is used for pushing the clamping head to extend out of the mounting hole,

through adopting above-mentioned technical scheme, when installing the ELIAS plate, at first push the joint towards in the mounting hole, the joint contracts into the mounting hole, installs the caulking groove with the ELIAS plate in the back, pushes the joint under the effect of spring and stretches out the mounting hole and support tightly with the ELIAS plate to improve the stability of ELIAS plate in the caulking groove.

Optionally, the joint is located the both sides protrusion of the one end in the mounting hole and is provided with the stopper, has seted up the spacing groove along the depth direction of mounting hole on the inner wall of mounting hole, the spacing groove is sealed towards mounting hole open-ended one end, and the stopper inlays to be located in the spacing groove and with the spacing groove cooperation of sliding.

Through adopting above-mentioned technical scheme, the stopper can guide and spacing sliding of joint, prevents the joint roll-off mounting hole, improves the stability and the smoothness degree that the joint slided.

Optionally, the upper surface of the card connector is an inclined surface inclined towards the bottom surface of the caulking groove, and the lower surface of the card connector is an inclined surface inclined towards the opening direction of the caulking groove.

By adopting the technical scheme, when the enzyme label plate is installed, the enzyme label plate is aligned with the caulking groove and pressed downwards, the clamping joint can be subjected to a component force in a sliding direction towards the installation hole in the process of pressing the enzyme label plate, so that the clamping joint is pressed into the installation hole, and when the enzyme label plate is installed in the caulking groove, the clamping joint tightly supports the enzyme label plate; after the washing is finished, the elisa plate is pulled outwards, and the clamping joint is subjected to a component force in a sliding direction towards the mounting hole in the process of pulling the elisa plate, so that the clamping joint is pressed into the mounting hole, and the elisa plate is taken out from the caulking groove; the convenience of installing and detaching the ELISA plate on the installation plate is improved.

In summary, the present application includes at least one of the following beneficial technical effects:

1. by arranging the washing head and the peristaltic pump, the convenience for washing the ELISA plate is improved, and the labor intensity of workers is reduced;

2. by arranging the driving assembly, the convenience of cleaning the ELISA plate is further improved, and the labor intensity of workers is reduced;

3. by arranging the lifting assembly, the convenience of cleaning the ELISA plate is further improved, and the labor intensity of workers is reduced;

4. through setting up the holder, improve the mounting panel and slide the process and wash the stability of ELIAS plate when ELIAS plate hole site.

Drawings

FIG. 1 is a schematic structural diagram of an ELISA plate cleaning device in the embodiment of the application;

FIG. 2 is a schematic structural diagram of a drive assembly in an embodiment of the present application;

FIG. 3 is a schematic view of the worm wheel and worm of the present embodiment;

FIG. 4 is a schematic structural view of a lift assembly in an embodiment of the present application;

FIG. 5 is a schematic structural diagram of the cooperation of the ELISA plate and the mounting plate in the embodiment of the application;

fig. 6 is a schematic structural view of a clamping assembly in an embodiment of the present application.

Description of reference numerals: 1. a host; 10. accommodating grooves; 11. a chute; 12. mounting grooves; 2. mounting a plate; 20. a slider; 21. caulking grooves; 22. mounting holes; 220. a limiting groove; 3. washing the head; 30. a liquid outlet needle; 31. a liquid suction needle; 4. a lotion bottle; 5. a waste liquid bottle; 6. a drive assembly; 60. driving a lead screw; 600. a worm gear; 61. a drive nut; 62. a drive motor; 620. a worm; 7. a lifting assembly; 70. lifting a screw rod; 71. a lifting nut; 710. a fixing plate; 72. a lifting motor; 8. a clamping member; 80. a clamping head; 800. a limiting block; 81. a spring; 9. an ELISA plate.

Detailed Description

The present application is described in further detail below with reference to figures 1-6.

The embodiment of the application discloses ELIAS plate belt cleaning device. Referring to fig. 1 and 2, the microplate cleaning device comprises a main machine 1 provided with a control panel, a mounting plate 2 which is matched with the main machine 1 in a transverse sliding manner is arranged on the main machine 1, a washing head 3 which is matched with the main machine 1 in a longitudinal sliding manner is arranged above the mounting plate 2 on the main machine 1, a plurality of liquid outlet needles 30 which are arranged in a matrix manner are distributed on one surface of the washing head 3 facing the mounting plate 2, liquid suction needles 31 are arranged on one side of each liquid outlet needle 30 in one-to-one correspondence with the corresponding liquid outlet needle 30, a washing liquid bottle 4 which is used for containing washing liquid and is communicated with the liquid outlet needle 30 and a waste liquid bottle 5 which is used for containing waste liquid and is communicated with the liquid suction needles 31 are arranged outside the main machine 1, and peristaltic pumps which are used for providing power for the washing liquid and providing vacuum degree for the liquid suction needles 31 are arranged in the main machine 1.

Referring to fig. 2 and 3, an accommodating groove 10 is formed in one side of the main unit 1 along the length direction of the main unit 1, the mounting plate 2 is arranged in the accommodating groove 10 and is in sliding fit with the accommodating groove 10, and a driving assembly 6 for driving the mounting plate 2 to slide along the accommodating groove 10 is arranged in the main unit 1; the bottom of the accommodating groove 10 is provided with a sliding groove 11 along the length direction of the accommodating groove 10, the driving assembly 6 comprises a driving lead screw 60 which is arranged in the sliding groove 11 and is in rotating fit with the sliding groove 11, a driving nut 61 which is arranged in the sliding groove 11 and is in rotating fit with the driving lead screw 60, and a driving motor 62 which is arranged in the host 1 and is used for driving the driving lead screw 60 to rotate; a sliding block 20 integrally cast with the mounting plate 2 is convexly arranged at the bottom of the mounting plate 2, the sliding block 20 is embedded in the sliding groove 11 and is in sliding fit with the sliding groove 11, and the driving nut 61 is fixedly connected with the sliding block 20; the drive motor 62 is a stepper motor; in order to reduce the pollution of washing liquor entering the sliding grooves 11 to the driving screw rods 60 and improve the sliding stability of the mounting plate 2, two sliding grooves 11 are symmetrically arranged on two sides of the accommodating groove 10, and two driving screw rods 60 are respectively arranged in the two sliding grooves 11; in order to facilitate the synchronous driving of the two driving screws 60, worm gears 600 are coaxially and fixedly connected to one ends of the two driving screws 60 facing the same direction, a worm 620 is coaxially and fixedly connected to an output end of the driving motor 62, and the worm 620 is engaged with the worm gears 600 on the two driving screws 60.

Referring to fig. 4, two passages are arranged in the washing head 3, one passage is communicated with the washing liquid bottle 4 and the liquid outlet needle 30, and the other passage is communicated with the waste liquid bottle 5 and the liquid suction needle 31; the length of the liquid suction needle 31 is greater than that of the liquid outlet needle 30, and a lifting component 7 for driving the washing head 3 to lift up and down is arranged on the main machine 1; an installation groove 12 extending along the vertical direction is arranged in the host 1, and the lifting assembly 7 comprises a lifting screw 70 vertically arranged in the installation groove 12 and in rotary fit with the installation groove 12, a lifting nut 71 matched with the lifting screw 70 and a lifting motor 72 for driving the lifting screw 70 to rotate; two ends of the lifting screw 70 are rotatably connected with two end surfaces of the mounting groove 12; the lifting nut 71 is in threaded connection with the lifting screw rod 70; the lifting motor 72 is a stepping motor, and the output end of the lifting motor is coaxially and fixedly connected with the lifting screw rod 70; a fixing plate 710 is protruded from the lifting nut 71, the fixing plate 710 and the lifting nut 71 are fixed by bolts, and the washing head 3 is fixedly connected to the fixing plate 710.

Referring to fig. 5 and 6, in order to improve the stability of the microplate 9 on the mounting plate 2, a clamping piece 8 for clamping the microplate 9 is provided on the mounting plate 2; the mounting plate 2 is provided with a caulking groove 21 matched with the enzyme label plate 9, and the inner wall of the caulking groove 21 is provided with a mounting hole 22; the clamping piece 8 comprises a clamping head 80 which is arranged in the mounting hole 22 and is matched with the mounting hole 22 in a sliding way, and a spring 81 which is arranged in the mounting hole 22 and is used for driving the clamping plate to extend out of the mounting hole 22; the clamping joint 80 is matched with the mounting hole 22, two sides of one end, located in the mounting hole 22, of the clamping joint 80 are convexly provided with limiting blocks 800, a limiting groove 220 is formed in the inner wall of the mounting hole 22 along the depth direction of the mounting hole 22, one end, facing the opening of the mounting hole 22, of the limiting groove 220 is closed, and the limiting blocks 800 are embedded in the limiting groove 220 and are in sliding fit with the limiting groove 220; two ends of the spring 81 are respectively abutted against the clamping joint 80 and the bottom end of the mounting hole 22, and the spring 81 is always in a compressed state; in order to improve the convenience of mounting and dismounting the elisa plate 9, the upper surface of the clamping joint 80 is an inclined surface inclined towards the bottom surface of the caulking groove 21, and the lower surface of the clamping joint 80 is an inclined surface inclined towards the opening direction of the caulking groove 21.

The application principle of the ELISA plate cleaning device provided by the embodiment of the application is as follows: when cleaning the ELISA plate 9, a worker firstly places the ELISA plate 9 on the mounting plate 2, and presses the ELISA plate 9 downwards by aligning the ELISA plate 9 with the caulking groove 21, the clamping joint 80 can receive a component force in a sliding direction towards the mounting hole 22 in the process of pressing the ELISA plate 9, so that the clamping joint 80 is pressed into the mounting hole 22, when the ELISA plate 9 is mounted in the caulking groove 21, the clamping joint 80 tightly supports the ELISA plate 9, then the position of the ELISA plate 9 is adjusted through the driving assembly 6, the driving motor 62 is started, the driving motor 62 drives the worm 620 to rotate, the worm 620 drives the two worm wheels 600 to synchronously rotate, and therefore the two driving lead screws 60 synchronously rotate; the driving screw 60 drives the driving nut 61 to slide, and the driving nut 61 drives the mounting plate 2 to slide, so that the hole position of the ELISA plate 9 corresponds to the washing head 3; then the height of the washing head 3 is adjusted by starting the lifting component 7, the lifting motor 72 is started, the lifting motor 72 drives the lifting screw 70 to rotate, the lifting screw 70 drives the lifting nut 71 to slide downwards, so that the washing head 3 descends, the liquid suction needle 31 is inserted into the hole position of the elisa plate 9, then the peristaltic pump is started, the peristaltic pump corresponding to the liquid outlet needle 30 is started, washing liquid is pressed into the position of the liquid outlet needle 30 from the washing liquid bottle 4 under the action of the peristaltic pump and flows into the hole position from the liquid outlet needle 30, after the washing liquid is soaked for a period of time, the peristaltic pump corresponding to the liquid suction needle 31 is started, the washing liquid in the hole position is sucked away and stored into the waste liquid bottle 5 under the action of the peristaltic pump, and then the operation is repeated until impurities in all the hole positions are cleaned.

After the washing is finished, the lifting motor 72 is started to drive the washing head 3 to ascend, the liquid sucking needle 31 is pulled out of the hole position, then the driving motor 62 is started to drive the mounting plate 2 to slide, the elisa plate 9 and the washing head 3 are staggered, the elisa plate 9 is pulled outwards, the clamping head 80 can be subjected to a component force in the sliding direction towards the mounting hole 22 in the process of pulling the elisa plate 9, and therefore the clamping head 80 is pressed into the mounting hole 22, and the elisa plate 9 is taken out of the caulking groove 21. In conclusion, the washing head 3 and the peristaltic pump are arranged, so that the convenience of washing the ELISA plate 9 is improved, and the labor intensity of workers is reduced.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. An ELIAS plate belt cleaning device which characterized in that: the washing machine comprises a host (1) provided with a control panel, a mounting plate (2) which is matched with the host (1) in a transverse sliding mode is arranged on the host (1), a washing head (3) which is matched with the host (1) in a longitudinal sliding mode is arranged above the mounting plate (2) on the host (1), a plurality of liquid outlet needles (30) which are arranged in a matrix mode are arranged on one surface, facing the mounting plate (2), of the washing head (3), liquid suction needles (31) are arranged on one side of each liquid outlet needle (30) in a one-to-one correspondence mode, a washing liquid bottle (4) which is used for containing washing liquid and communicated with the liquid outlet needles (30) and a waste liquid bottle (5) which is used for containing waste liquid and communicated with the liquid suction needles (31) are arranged outside the host (1), and a peristaltic pump which is used for providing power for the washing liquid and providing vacuum degree for the liquid suction needles (31) is arranged in the host (1).

2. The microplate cleaning device of claim 1, wherein: a driving component (6) for driving the mounting plate (2) to slide is arranged in the main machine (1); a sliding groove (11) is formed in the main machine (1) along the sliding direction of the mounting plate (2), the driving assembly (6) comprises a driving lead screw (60) which is arranged in the sliding groove (11) and is in rotating fit with the sliding groove (11), a driving nut (61) which is matched with the driving lead screw (60) and a driving motor (62) which is used for pushing the driving lead screw (60) to rotate; be provided with slider (20) on mounting panel (2), slider (20) inlay locate in spout (11) and with spout (11) sliding fit, slider (20) and drive nut (61) rigid coupling.

3. The microplate cleaning device of claim 2, wherein: a lifting component (7) for driving the washing head (3) to slide up and down is arranged in the main machine (1); the washing machine is characterized in that an installation groove (12) is formed in the main machine (1) along the sliding direction of the washing head (3), the lifting assembly (7) comprises a lifting screw rod (70) which is arranged in the installation groove (12) and is in running fit with the main machine (1), a lifting nut (71) which is matched with the lifting screw rod (70) and a lifting motor (72) which is used for driving the lifting screw rod (70) to rotate, and the washing head (3) is connected with the lifting nut (71).

4. The microplate cleaning device of claim 2, wherein: the two sliding grooves (11) are symmetrically arranged on two sides of the washing head (3), and the two driving screw rods (60) are respectively arranged in the sliding grooves (11).

5. The microplate cleaning device of claim 4, wherein: two drive lead screw (60) are towards the same direction one end coaxial rigid coupling respectively and are had worm wheel (600), and coaxial rigid coupling has worm (620) at the output of driving motor (62), and worm (620) and two worm wheel (600) meshes on drive lead screw (60).

6. The microplate cleaning device of claim 4, wherein: the mounting plate (2) is provided with a caulking groove (21) matched with the enzyme label plate (9).

7. The microplate cleaning device of claim 6, wherein: the mounting plate (2) is provided with a clamping piece (8) for clamping and fixing the ELISA plate (9).

8. The microplate cleaning device of claim 7, wherein: the inner wall of the caulking groove (21) is provided with a mounting hole (22); the clamping piece (8) comprises a clamping head (80) which is arranged in the mounting hole (22) and is in sliding fit with the mounting hole (22) and a spring (81) which is arranged in the mounting hole (22) and used for pushing the clamping head (80) to stretch out of the mounting hole (22).

9. The microplate cleaning device of claim 8, wherein: the both sides protrusion that joint (80) were located one end in mounting hole (22) is provided with stopper (800), has seted up spacing groove (220) along the depth direction of mounting hole (22) on the inner wall of mounting hole (22), spacing groove (220) are sealed towards mounting hole (22) open-ended one end, and stopper (800) inlay locate in spacing groove (220) and with spacing groove (220) cooperation of sliding.

10. The microplate cleaning device of claim 8, wherein: the upper surface of the clamping head (80) is an inclined surface inclined towards the bottom surface of the caulking groove (21), and the lower surface of the clamping head (80) is an inclined surface inclined towards the opening direction of the caulking groove (21).