CN208999443U - A kind of immunoblotting assay instrument - Google Patents
A kind of immunoblotting assay instrument Download PDFInfo
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- CN208999443U CN208999443U CN201821408336.1U CN201821408336U CN208999443U CN 208999443 U CN208999443 U CN 208999443U CN 201821408336 U CN201821408336 U CN 201821408336U CN 208999443 U CN208999443 U CN 208999443U
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Abstract
The utility model provides a kind of immunoblotting assay instrument, comprising: sample film clamping device；Reciprocating mechanism drives the sample film clamping device to move up and down；Anterior-posterior translation mechanism drives the sample film clamping device to be moved forward and backward；Pallet, upper surface are provided with washing slot, multiple room temperature slots, multiple low temperature slots；Wash filling mechanism；Wash Qi Ye mechanism.Compared with the prior art, the waste liquid after sample film is only washed in sink is rejected the utility model, and the primary antibody secondary antibody test solution of high price can be repeated and efficiently utilized, experimental cost is greatly saved；It in incubation period, drives sample film to vibrate repeatedly up and down in test solution box by reciprocating mechanism, test solution and sample film can be promoted to carry out blending incubation, be sufficiently immunoreacted.
Description
Technical field
The utility model relates to a kind of experiment auxiliary appliance, especially a kind of immunoblotting assay instrument.
Background technique
Western blot test is also known as Western Blot Assay (Western Blot).It is biochemistry and immunogenetics
In a kind of common experimental method, be that the cell or tissue gross protein after electrophoretic separation is transferred to solid phase carrier from gel
On NC film or pvdf membrane, then using on solid phase carrier protein or polypeptide as antigen, play immune response with corresponding antibody, then
It reacts with the secondary antibody of enzyme or isotope labelling, finally by substrate colour developing or autoradiograph to detect electrophoretic separation
The protein ingredient of specific destination gene expression.In entire immune response experiment, need to carry out more test solutions to solid phase carrier film
Cleaning-incubation-cleaning operation of multifrequency time, time-consuming, and entering frequency is high, experimenter's great work intensity.
The automatic lmunoassays analyzer majority used in Hospitals at Present is large scale equipment, although test sample in the unit time
It is more, but structure is complicated, and price is expensive, and a small amount of sample detection of single is generally in laboratory, and sample specification is not also identical, therefore
The not use in appropriate experimental room.
Sample, is usually placed in reaction utensil by the small-sized immunoblotting assay instrument used in laboratory at present, experiment
In the process, test solution is added in reaction utensil, test solution is individually sucked out by imbibition rifle again after reaction and is cleaned, in quilt
In the primary antibody test solution and secondary antibody test solution given up includes the antibody not being consumed largely, causes the serious wave of primary antibody and secondary antibody
Take, and the price of primary antibody and secondary antibody is costly, results in higher experimental cost.
Utility model content
In order to overcome the above-mentioned deficiencies of the prior art, the purpose of the utility model is to provide a kind of immunoblotting assays
Instrument.
In order to achieve the above objectives, the technical scheme adopted by the utility model to solve the technical problem is as follows:
A kind of immunoblotting assay instrument, comprising:
Frame；
Sample film clamping device is used to clamp the sample film comprising protein to be analyzed or polypeptide；
Reciprocating mechanism drives the sample film clamping device to move up and down in frame；
Anterior-posterior translation mechanism drives the sample film clamping device to be moved forward and backward in frame；
Pallet, upper surface, which is provided with, washs slot, multiple room temperature slots, multiple low temperature slots, in the washing slot
It is inserted with sink, the room temperature slot is used to be plugged in the test solution box of normal temperature condition reaction, and the low temperature slot is for plugging
Temperature-controlling component is equipped in the test solution box of cryogenic conditions reaction, the low temperature slot；
Filling mechanism is washed, is used to add cleaning solution in sink；
Qi Ye mechanism is washed, is used to for cleaning solution in sink being discharged.
The utility model compared to the prior art, by sample film gripper mechanism grips sample film to be analyzed, according to experimental procedure
Program, it is mobile between each test solution box on sink and room temperature slot and low temperature slot to complete washing incubation, only washing
It washs the waste liquid after being washed in box to be rejected, the primary antibody secondary antibody test solution of high price can be repeated and efficiently utilized, saved significantly
About experimental cost；In incubation period, drives sample film to vibrate repeatedly up and down in test solution box by reciprocating mechanism, can promote
Test solution and sample film carry out blending incubation, are sufficiently immunoreacted.
Further, the anterior-posterior translation mechanism include on frame along the longitudinal direction horizontally disposed guide rail, driving horse
It reaches, drive gear, driven gear, band tooth transmission belt and vertical gripper shoe, the drive motor drives the rotation of sliding tooth wheel, described
Band tooth transmission belt is set on driving gear and driven gear, and the sliding block of the guide rail is connected on band tooth transmission belt, described perpendicular
Allotment fagging is mounted on the sliding block of guide rail.
Further, the reciprocating mechanism includes the sliding rail being vertically arranged in vertical gripper shoe, is vertically arranged
Screw rod, screw motor, feed screw nut and the first transverse plate, first transverse plate are connected to sliding block and the feed screw nut of sliding rail
On, the screw motor driving screw rod rotates and then the first transverse plate is driven to move up and down along sliding rail.
Using the above preferred scheme, operation is more steady, and position precision is higher, can be driven by sample film clamping device
Sample film carries out stable front and back and up and down action.
Further, the sample film clamping device includes the second transverse plate, lateral pressing plate, film carrier piece and pressuring film plate, described
Second transverse plate is removably connected by closure and the first transverse plate, and second transverse plate is equidistantly set equipped with multiple
The top of the positioning column set, the film carrier piece and pressuring film plate is equipped at least two and the matched location hole of positioning column, and sample film is placed with
In film carrier on piece, pressuring film plate is pressed in the top position of sample film, and the transverse direction pressing plate passes through closure for pressuring film plate, sample film and film carrier
Piece is compressed and is suspended vertically below the second transverse plate.
Using the above preferred scheme, sample back of the membrane is attached to film carrier on piece, and pressuring film plate pushes down the positive top of sample film, and three
Person is placed to together on the positioning column of the second transverse plate, is compressed finally by lateral pressing plate, is realized effective fixation of sample film, the
The sample film that different size can be placed on two transverse plates, facilitates the use in laboratory.
Further, the washing slot on the pallet, room temperature slot, low temperature slot are equipped with for plugging test solution box
Flat groove body, the flat groove body side wall are equipped with multiple locating slots equidistantly arranged, sink and test solution box be equipped with
The matched positioning strip of locating slot.
Using the above preferred scheme, sink and test solution slot can be preferably in washing slot, room temperature slot and low temperature
It is positioned on slot, it is ensured that stablize position；The sink of suitable size and test solution box can be selected to carry out according to the size of sample film slotting
With installation, to reduce the use of cleaning solution and each test solution to greatest extent.
Further, the pallet two sides are equipped with guide rail, and pallet can be along guide rail anterior-posterior translation.
Using the above preferred scheme, convenient for pulling out the inserting that pallet carries out each test solution box on the outside of frame, operation is improved
Convenience.
Further, the washing filling mechanism includes cleaning solution bucket, liquid pump, liquid supply pipe, liquid feeding head and left and right translation machine
Structure, the left and right translation mechanism include the guide rail horizontally disposed in left-right direction being arranged on cruciform bracing, are arranged in crossbeam
On driving gear, driven gear, band tooth transmission belt, drive motor and liquid feeding bracket, band tooth transmission belt is set in driving gear
Between driven gear, the sliding block of guide rail is connected on band tooth transmission belt, and the liquid feeding bracket is fixed on the sliding block of guide rail；Institute
It states liquid feeding head and liquid pump is arranged on liquid feeding bracket, the liquid pump is connected to cleaning solution bucket through liquid supply pipe, and the liquid feeding head is corresponding
In the top of sink, the drive motor drives the rotation of sliding tooth wheel, through band tooth transmission V belt translation and then liquid feeding head is driven to exist
It is moved left and right above each sink on washing slot.
Using the above preferred scheme, it needs to carry out sample film using 1 × TBST cleaning solution in immunoblot experiment more
Secondary cleaning drives liquid feeding head to carry out liquid feeding respectively above each sink, can save cleaning solution by left and right translation mechanism
Use, improve washing effect.
Further, the washing slot bottom is equipped with the through slot for penetrating into tray bottom, and the sink bottom is equipped with
Lateral leakage fluid dram, the washing Qi Ye mechanism includes sealing press strip, cam, motor, lateral on the sealing press strip and sink
The matched position of leakage fluid dram is equipped with sealing element, and the sealing press strip two sides are equipped with sagittal guiding axis, set on the guiding axis
Have and promote to seal the reset spring that press strip is separated with lateral leakage fluid dram, the cam is contradicted in the middle position of sealing press strip, institute
It states motor band moving cam to rotate, realizes that sealing press strip is sealed and separated with the conflict of lateral leakage fluid dram.
Further, waste liquid collecting box is equipped with below the lateral leakage fluid dram of sink.
Using the above preferred scheme, it when washing, is rotated by motor band moving cam, realizes sealing press strip and sink side
It is closed to the compression of leakage fluid dram；After the completion of washing, band moving cam rotates motor again, under the tensile force of reset spring, realizes envelope
The separation of mouth press strip and lateral leakage fluid dram, scrub raffinate flow out in waste liquid collecting box.
Further, the sample film clamping device is equipped with the first sensor group of detection sample film clip position and quantity,
The washing slot is equipped with detection sink size, the second sensor group of quantity and installation site, the room temperature slot, low
Warm slot, which is equipped with, is used for detection test solution box size, the 3rd sensor group of quantity and installation site, and the test solution box is equipped with
The graphic code for recording test solution type is additionally provided on the room temperature slot, low temperature slot for acquiring sweeping for graphic code on test solution box
Retouch a group；
Using the above preferred scheme, by the detection information of each sensor, whether control system determines each test solution automatically
It is reasonably placed, and sample film is moved in required test solution box automatically according to experimental arrangement and is immunoreacted, improved point
The simplification of analyzer operation.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 2 is a kind of structural schematic diagram of embodiment of the utility model；
Fig. 3 is the structural schematic diagram of anterior-posterior translation mechanism and reciprocating mechanism in a kind of embodiment of the utility model；
Fig. 4 is the structural schematic diagram of sample film clamping device in a kind of embodiment of the utility model；
Fig. 5 is the structural schematic diagram of sample film clamping device in a kind of embodiment of the utility model；
Fig. 6 is the structural schematic diagram of pallet in a kind of embodiment of the utility model；
Fig. 7 is the structural schematic diagram of test solution box in a kind of embodiment of the utility model；
Fig. 8 is the structural schematic diagram of test solution box in the utility model another embodiment；
Fig. 9 is the structural schematic diagram of sink in a kind of embodiment of the utility model；
Figure 10 is the structural schematic diagram of left and right translation mechanism in a kind of embodiment of the utility model；
Figure 11 is the structural schematic diagram that Qi Ye mechanism is washed in a kind of embodiment of the utility model；
Figure 12 is a kind of structural schematic diagram of embodiment of the utility model.
The title of number and corresponding component represented by letter in figure:
1- frame；11- crossbeam；2- sample film clamping device；The second transverse plate of 21-；211- positioning column；22- transverse direction pressing plate；
23- film carrier piece；24- pressuring film plate；25- sample film；3- reciprocating mechanism；31- sliding rail；32- screw rod；33- screw motor；34-
Stem nut；The first transverse plate of 35-；4- anterior-posterior translation mechanism；41- guide rail；42- drive motor；43- drives gear；44- driven tooth
Wheel；45- band tooth transmission belt；46- vertical gripper shoe；47- sliding block；5- pallet；51- washs slot；52- room temperature slot；53- low temperature
Slot；54- sink；The side 541- is to leakage fluid dram；55- test solution box；56- locating slot；57- positioning strip；58- guide rail；59- through slot；
6- washs filling mechanism；61- cleaning solution bucket；62- liquid pump；63- liquid supply pipe；64- liquid feeding head；7- or so translation mechanism；71- is led
Rail；72- drives gear；73- driven gear；74- band tooth transmission belt；75- drive motor；76- liquid feeding bracket；Liquid machine is abandoned in 8- washing
Structure；81- seals press strip；82- cam；83- motor；84- guiding axis；85- reset spring.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, fall within the protection scope of the utility model.
As shown in figures 1 to 6, a kind of immunoblotting assay instrument, comprising:
Frame 1；
Sample film clamping device 2 is used to clamp the sample film comprising protein to be analyzed or polypeptide；
Reciprocating mechanism 3 drives sample film clamping device 2 to move up and down in frame 1；
Anterior-posterior translation mechanism 4 drives sample film clamping device 2 to be moved forward and backward in frame 1；
Pallet 5, upper surface are provided with washing slot 51, multiple room temperature slots 52, multiple low temperature slots 53, wash slot
Sink 54 is inserted in 51, room temperature slot 52 is used to be plugged in the test solution box of normal temperature condition reaction, and low temperature slot 53 is for inserting
It is located at the test solution box of cryogenic conditions reaction, is equipped with temperature-controlling component in low temperature slot 53；
Filling mechanism 6 is washed, is used to add cleaning solution in sink 54；
Qi Ye mechanism 8 is washed, is used to for cleaning solution in sink 54 being discharged.
Beneficial effect by adopting the above technical scheme is: sample film to be analyzed is clamped by sample film clamping device 2, according to experiment
Step procedure, it is mobile between each test solution box on sink and room temperature slot and low temperature slot to complete washing incubation, only
Waste liquid after being washed in sink is rejected, and the primary antibody secondary antibody test solution of high price can be repeated and efficiently utilized, greatly
Experimental cost has been saved greatly；In incubation period, sample film is driven to vibrate repeatedly up and down in test solution box by reciprocating mechanism, energy
Promote test solution and sample film to carry out blending incubation, is sufficiently immunoreacted.
As shown in figure 3, anterior-posterior translation mechanism 4 includes the edge on frame 1 in other embodiments of the utility model
The horizontally disposed guide rail 41 of front-rear direction, drive motor 42, driving gear 43, driven gear 44, band tooth transmission belt 45 and vertical
Support plate 46, drive motor 42 drive driving gear 43 to rotate, and band tooth transmission belt 45 is set in driving gear 43 and driven gear
On 44, the sliding block 47 of guide rail 41 is connected on band tooth transmission belt 45, and vertical gripper shoe 46 is mounted on the sliding block 47 of guide rail 41.On
Lower mobile mechanism 3 include be vertically arranged in vertical gripper shoe 46 sliding rail 31, be vertically arranged screw rod 32, screw motor 33,
Feed screw nut 34 and the first transverse plate 35, the first transverse plate 35 are connected on the sliding block and feed screw nut 34 of sliding rail 31, screw rod horse
It is rotated up to 33 driving screw rods 32 and then the first transverse plate 35 is driven to move up and down along sliding rail 31.By adopting the above technical scheme beneficial
Effect is: operation is more steady, and position precision is higher, can be driven by sample film clamping device sample film carry out stable front and back and
It moves up and down.
As shown in figures 1,4 and 5, in other embodiments of the utility model, sample film clamping device 2 includes second horizontal
It is removable by closure and the first transverse plate 35 to plate 21, lateral pressing plate 22, film carrier piece 23 and pressuring film plate 24, the second transverse plate 21
The connection of dress formula, the second transverse plate 21 are equipped with the positioning column 211 of multiple spaced sets, the top of film carrier piece 23 and pressuring film plate 24
Equipped at least two and the matched location hole of positioning column 211, sample film 25 is placed on film carrier piece 23, and pressuring film plate 24 is pressed in sample film 25
Top position, pressuring film plate 24, sample film 25 and film carrier piece 23 compressed by closure and be suspended vertically the by lateral pressing plate 22
Two transverse plates, 21 lower section.Beneficial effect by adopting the above technical scheme is: 25 back side of sample film is attached on film carrier piece 23, pressuring film plate
24 push down the positive top of sample film 25, and three is placed to together on the positioning column 211 of the second transverse plate 21, finally by lateral pressure
Plate 22 compresses, and realizes effective fixation of sample film 25, the sample film 25 of different size can be placed on the second transverse plate 21, need to only change
The film carrier piece 23 of respective model, facilitates the use in laboratory.
As Figure 6-9, in other embodiments of the utility model, washing slot 51, room temperature on pallet 5 are inserted
Slot 52, low temperature slot 53 are equipped with flat groove body for plugging sink 54 and test solution box 55, on the flat groove body side wall
Equipped with multiple locating slots 56 equidistantly arranged, sink 54 and test solution box 55 are equipped with and the matched positioning strip of locating slot 56
57.Beneficial effect by adopting the above technical scheme is: sink 54 and test solution slot 55 can be preferably in washing slots 51, room temperature
It is positioned on slot 52 and low temperature slot 53, it is ensured that stablize position；The sink of suitable size can be selected according to the size of sample film
54 and test solution box 55 carry out inserting installation, to reduce the use of cleaning solution and each test solution to greatest extent.Fig. 7 and Fig. 8 are shown not
With the test solution box of size, the positioning in slot is all realized by positioning strip 57.
In other embodiments of the utility model, on the room temperature slot and low temperature slot that are arranged on pallet respectively
The label of common test solution type is set, and experimenter marks inserting test solution box according to this.
As shown in Fig. 2, 5 two sides of pallet are equipped with guide rail 58, and pallet 5 can in other embodiments of the utility model
Along 58 anterior-posterior translation of guide rail.Beneficial effect by adopting the above technical scheme is: each examination is carried out on the outside of frame convenient for pulling out pallet 5
The inserting of liquid box improves operation ease.
As shown in Fig. 1,10, in other embodiments of the utility model, washing filling mechanism 6 includes cleaning solution bucket
61, liquid pump 62, liquid supply pipe 63, liquid feeding head 64 and left and right translation mechanism 7, left and right translation mechanism 7 include being arranged in cruciform bracing 11
On guide rail 71 horizontally disposed in left-right direction, be arranged on crossbeam driving gear 72, driven gear 73, band tooth transmission belt
74, drive motor 75 and liquid feeding bracket 76, band tooth transmission belt 74 are set between driving gear 72 and driven gear 73, guide rail 71
Sliding block be connected to band tooth transmission belt 74 on, liquid feeding bracket 76 is fixed on the sliding block of guide rail 71；Liquid feeding head 64 and liquid pump 62 are set
It sets on liquid feeding bracket 76, liquid pump 62 is connected to cleaning solution bucket 61 through liquid supply pipe 63, and liquid feeding head 64 is correspondingly arranged at the upper of sink
Side, drive motor 75 drive driving gear 72 to rotate, and are driven through band tooth transmission belt 74 and then drive liquid feeding head 64 in washing slot
It is moved left and right above each sink 54 on 51.Beneficial effect by adopting the above technical scheme is: needing in immunoblot experiment
Sample film is cleaned multiple times using 1 × TBST cleaning solution, drive liquid feeding head 64 in each washing by left and right translation mechanism 7
54 top of box carries out liquid feeding respectively, can save the use of cleaning solution, improve washing effect.
As shown in Fig. 9,11, in other embodiments of the utility model, washing slot bottom, which is equipped with, penetrates into support
The through slot 59 of pan bottom, 54 bottom of sink are equipped with lateral leakage fluid dram 541, and washing Qi Ye mechanism 8 includes sealing press strip 81, cam
82, the matched position of lateral leakage fluid dram 541 of motor 83, sealing press strip 81 and sink is equipped with sealing element, seals 81 liang of press strip
Side is equipped with sagittal guiding axis 84, and guiding axis 84, which is equipped with, promotes to seal the reset isolated with lateral leakage fluid dram 541 of press strip 81
Spring 85, cam 82 contradict sealing press strip 81 middle position, motor 83 with moving cam 82 rotate, realize sealing press strip 81 with
The conflict sealing and separation of lateral leakage fluid dram 541；Waste liquid collecting box is equipped with below the lateral leakage fluid dram 541 of sink 54.It adopts
It is with the beneficial effect of above-mentioned technical proposal: when washing, is rotated by motor 83 with moving cam 82, realize sealing press strip 81 and wash
Wash the compression closing of the lateral leakage fluid dram 541 of box；After the completion of washing, band moving cam 82 rotates motor 83 again, in reset spring 85
Under tensile force, the separation of sealing press strip 81 and lateral leakage fluid dram 541 is realized, scrub raffinate flows out in waste liquid collecting box.
Below in conjunction with a kind of immunoblot experiment process of biotin mark secondary antibody, illustrate the movement of immunoblotting assay instrument
Journey.
The immunoblot experiment flow table of biotin mark secondary antibody:
The operation of immunoblotting assay instrument and action process:
1. adding enough No. 1 liquid 1 × TBST cleaning solutions in the washing fluid cylinder in washing filling mechanism；
2. clamping sample film side by side on the second transverse plate；
3. pulling out pallet, two rows of room temperature slots, by sample film quantity and size, the inserting side by side in a wherein slot are chosen
No. 2 liquid boxes of confining liquid are matched and are loaded with sample film, and inserting matches and is contained with sample film side by side in another slot
No. 5 liquid boxes of secondary antibody diluent；Three row's low temperature slots are chosen, equally press sample film quantity and size, wherein a low temperature slot
Inside inserting and sample film match and are contained with No. 3 liquid boxes of primary antibody dilution and are contained with No. 4 liquid of 1%BSA comparison liquid side by side
Box, inserting is contained with No. 6 liquid boxes of Avidin dilution and is contained with biotinylation respectively in other two low temperature slots
No. 7 liquid boxes of enzyme dilution；
4. selecting corresponding program in immunoblotting assay instrument and starting, reciprocating mechanism and anterior-posterior translation mechanism are dynamic
Make, sample film be moved in sink and is washed, is placed cleaning solution 3 times by washing filling mechanism and washing tapping mechanism,
Washing 5 minutes every time；Sample film is moved in No. 2 liquid boxes again, is reacted with confining liquid 2 hours, sample film is moved to washes after the completion
It washs and is washed in box liquid 3 times, every time washing 5 minutes；Sample film is moved in No. 3 liquid boxes and No. 4 liquid boxes arranged side by side again, in primary antibody
It is reacted 8 hours in dilution and 1%BSA comparison liquid, during which in order to constantly mix test solution, at interval of 10 minutes, moves up and down machine
Structure drives sample film 5 bout of but small oscillations up and down, sample film is moved in sink washs liquid 3 times after the completion, every time washing 5 minutes；
Sample film is moved in No. 5 liquid boxes again, is reacted 2 hours in secondary antibody diluent, sample film is moved in sink washs after the completion
It liquid 4 times, washs 10 minutes every time；It is will be moved into No. 6 liquid boxes again, reacts in Avidin dilution 45 minutes, after the completion will
Sample film, which is moved in sink, to be washed liquid 4 times, every time washing 5 minutes；It will be moved into No. 7 liquid boxes, diluted in biotinylation enzyme again
It is reacted in liquid 45 minutes, sample film is moved in sink washs liquid 4 times after the completion, every time washing 5 minutes, that is, complete exempting from for sample film
The experiment of epidemic disease engram analysis, has obtained the sample film and a 1%BSA control experiment sample film of 1 primary antibody reaction.
As shown in figure 12, a kind of immunoblotting assay control system, based on above-mentioned immunoblotting assay instrument, the sample
Film clamping device is equipped with the first sensor group of detection sample film clip position and quantity, and the washing slot is washed equipped with detection
The second sensor group of box size, quantity and installation site is washed, the room temperature slot, low temperature slot, which are equipped with, is used for detection test solution
The 3rd sensor group of box size, quantity and installation site, the test solution box is equipped with the graphic code of record test solution type, described
The probe group for acquiring graphic code on test solution box is additionally provided on room temperature slot, low temperature slot；Immunoblotting assay control system
System includes:
Program Edit Module is used to input or edit immunoblot experiment program；
Procedure selection module is used for the experimental arrangement carried out needed for selecting；
Test solution checks module, is used to check on room temperature slot and low temperature slot institute in the type of test solution box and experimental arrangement
Test solution is needed to match, whether test solution box position matches with sample film location；
Action triggers module is used for after the test solution box information matches that the test solution checks module check are correct, according to
Experimental arrangement, controls reciprocating mechanism and anterior-posterior translation mechanism drives sample film to act between each test solution box and sink, and controls
System washing filling mechanism and washing Qi Ye mechanism carry out the addition and discharge of cleaning solution to sink.
Beneficial effect by adopting the above technical scheme is: by the detection information of each sensor, control system determines automatically
Whether each test solution is reasonably placed, and sample film is moved in required test solution box automatically according to experimental arrangement and be immunized instead
It answers, improves the simplification of analyzer operation.
The above embodiments are only for explaining the technical ideas and features of the present invention, and its object is to allow the common skill in this field
Art personnel can understand the content of the utility model and be implemented, and not limit the protection scope of the present invention,
All equivalent change or modifications according to made by the spirit of the present invention essence, should all cover in the protection scope of the utility model
It is interior.
Claims (9)
1. a kind of immunoblotting assay instrument characterized by comprising
Frame；
Sample film clamping device is used to clamp the sample film comprising protein to be analyzed or polypeptide；
Reciprocating mechanism drives the sample film clamping device to move up and down in frame；
Anterior-posterior translation mechanism drives the sample film clamping device to be moved forward and backward in frame；
Pallet, upper surface are provided with washing slot, multiple room temperature slots, multiple low temperature slots, plug in the washing slot
There is sink, the room temperature slot is used to be plugged in the test solution box of normal temperature condition reaction, and the low temperature slot is low for being plugged in
The test solution box of warm conditioned response, the low temperature slot is interior to be equipped with temperature-controlling component；
Filling mechanism is washed, is used to add cleaning solution in sink；
Qi Ye mechanism is washed, is used to for cleaning solution in sink being discharged.
2. immunoblotting assay instrument according to claim 1, which is characterized in that the anterior-posterior translation mechanism is included in frame
On along the longitudinal direction horizontally disposed guide rail, drive motor, driving gear, driven gear, band tooth transmission belt and vertical gripper shoe,
The drive motor drives the rotation of sliding tooth wheel, and the band tooth transmission belt is set on driving gear and driven gear, described to lead
The sliding block of rail is connected on band tooth transmission belt, and the vertical gripper shoe is mounted on the sliding block of guide rail.
3. immunoblotting assay instrument according to claim 2, which is characterized in that the reciprocating mechanism includes setting vertically
Screw rod, screw motor, feed screw nut and the first transverse plate setting the sliding rail in vertical gripper shoe, being vertically arranged, described first
Transverse plate is connected on the sliding block and feed screw nut of sliding rail, and the screw motor driving screw rod rotates and then drives the first transverse plate
It is moved up and down along sliding rail.
4. immunoblotting assay instrument according to claim 3, which is characterized in that the sample film clamping device includes second horizontal
Pass through closure and the dismountable company of the first transverse plate to plate, lateral pressing plate, film carrier piece and pressuring film plate, second transverse plate
It connects, positioning column of second transverse plate equipped with multiple spaced sets, the top of the film carrier piece and pressuring film plate is equipped with extremely
Few two are placed with the matched location hole of positioning column, sample film in film carrier on piece, and pressuring film plate is pressed in the top position of sample film, the cross
Pressuring film plate, sample film and film carrier piece are compressed by closure to pressing plate and are suspended vertically below the second transverse plate.
5. immunoblotting assay instrument according to claim 1, which is characterized in that washing slot, room temperature on the pallet
Slot, low temperature slot are equipped with the flat groove body for plugging test solution box, and the flat groove body side wall is equipped with multiple equidistant
The locating slot of arrangement, sink and test solution box are equipped with and the matched positioning strip of locating slot.
6. immunoblotting assay instrument according to claim 5, which is characterized in that the pallet two sides are equipped with guide rail, pallet
It can be along guide rail anterior-posterior translation.
7. immunoblotting assay instrument according to claim 1, which is characterized in that the washing filling mechanism includes cleaning solution
Bucket, liquid pump, liquid supply pipe, liquid feeding head and left and right translation mechanism, the left and right translation mechanism include the edge being arranged on cruciform bracing
The horizontally disposed guide rail of left and right directions, the driving gear being arranged on crossbeam, driven gear, band tooth transmission belt, drive motor and
Liquid feeding bracket, band tooth transmission belt are set between driving gear and driven gear, and the sliding block of guide rail is connected on band tooth transmission belt,
The liquid feeding bracket is fixed on the sliding block of guide rail；The liquid feeding head and liquid pump are arranged on liquid feeding bracket, and the liquid pump is through supplying
Liquid pipe is connected to cleaning solution bucket, and the liquid feeding head corresponds to the top of sink, and the drive motor drives the rotation of sliding tooth wheel,
Through driving liquid feeding head to move left and right above each sink on washing slot in turn with tooth transmission V belt translation.
8. immunoblotting assay instrument according to claim 7, which is characterized in that the washing slot bottom is equipped with and penetrates into
The through slot of tray bottom, the sink bottom are equipped with lateral leakage fluid dram, and the washing Qi Ye mechanism includes sealing press strip, convex
The lateral matched position of leakage fluid dram is equipped with sealing element, the sealing press strip two sides on wheel, motor, the sealing press strip and sink
Equipped with sagittal guiding axis, the guiding axis, which is equipped with, promotes to seal the reset spring that press strip is separated with lateral leakage fluid dram, institute
It states cam to contradict in the middle position of sealing press strip, the motor band moving cam rotates, and realizes sealing press strip and lateral leakage fluid dram
Conflict sealing and separation.
9. immunoblotting assay instrument according to claim 8, which is characterized in that set below the lateral leakage fluid dram of sink
There is waste liquid collecting box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821408336.1U CN208999443U (en) | 2018-08-30 | 2018-08-30 | A kind of immunoblotting assay instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821408336.1U CN208999443U (en) | 2018-08-30 | 2018-08-30 | A kind of immunoblotting assay instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208999443U true CN208999443U (en) | 2019-06-18 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109187990A (en) * | 2018-08-30 | 2019-01-11 | 莫纳（苏州）生物科技有限公司 | A kind of immunoblotting assay instrument and control system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109187990A (en) * | 2018-08-30 | 2019-01-11 | 莫纳（苏州）生物科技有限公司 | A kind of immunoblotting assay instrument and control system |
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