CN108414522A - The device of all-in-one machine is detected for micro-fluidic chip and the more flux of NC films - Google Patents

The device of all-in-one machine is detected for micro-fluidic chip and the more flux of NC films Download PDF

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Publication number
CN108414522A
CN108414522A CN201810410729.4A CN201810410729A CN108414522A CN 108414522 A CN108414522 A CN 108414522A CN 201810410729 A CN201810410729 A CN 201810410729A CN 108414522 A CN108414522 A CN 108414522A
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chip
films
module
slot
sample introduction
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CN108414522B (en
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许行尚
杰弗瑞·陈
应家树
张前军
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Nanjing Lanyu Biological Technology Co Ltd
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Nanjing Lanyu Biological Technology Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/8422—Investigating thin films, e.g. matrix isolation method
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G01N2001/386—Other diluting or mixing processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00554—Mixing by a special element, e.g. stirrer using ultrasound

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  • Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Mathematical Physics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention discloses a kind of devices detecting all-in-one machine for micro-fluidic chip and the more flux of NC films, include pedestal, sample introduction module is provided on the pedestal, incubates module, cleaning module, NC films detection module and chip detection module；Wherein, it is provided with mutually independent chip sample introduction card slot and NC film sample introduction card slots in the sample introduction module, is respectively for insertion into chip or NC film strips；Fluid path gas path module is additionally provided in the sample introduction module；There is mutually independent chip to incubate slot and NC films incubation slot, and the side of the sample introduction module, cleaning module and NC films detection module on the incubation block length direction is arranged in the incubation module；Chip after being cleaned in the cleaning module, which is sent into the chip detection module, to be detected.Modules component is incorporated on pedestal and forms complete equipment, it is structure-integrated, take into account the Technology application scene of micro-fluidic chip and the detection of NC films.

Description

The device of all-in-one machine is detected for micro-fluidic chip and the more flux of NC films
Technical field
The invention belongs to technical field of medical equipment, are examined for micro-fluidic chip and the more flux of NC films more particularly, to one kind Survey the device of all-in-one machine.
Background technology
Micro-fluidic chip (microfluidic chip) is current micro-total analysis system (Miniaturized Total Analysis Systems) development hot fields.Microfluidic chip analysis is using chip as operating platform, while with analytical chemistry Based on, it is to rely on micro electro mechanical processing technology, is main application at present with life science using microchannel network as structure feature Object is the emphasis of current micro-total analysis system field development.Its target is the function entire laboratory, including sampling, Dilution, reagent adding, reaction, separation, detection etc. are integrated on microchip.Micro-fluidic chip is the main flat of microflow control technique realization Platform.Its device characteristic is mainly that the resulting structure (channel, reative cell and other certain functional components) of its receiving fluid at least exists It is micron order scale on one latitude.Due to micron-sized structure, fluid shows and produces wherein different from macro-scale Property.Therefore the performance that unique analysis generates is developed.The characteristics of micro-fluidic chip and advantages for development：Micro-fluidic core Piece has the characteristics that liquid flowing is controllable, consumption sample and reagent are few, improves to analyze speed tenfold hundreds of times, it can be with It is analyzed while carrying out a sample up to a hundred within a few minutes or even shorter time, and can be with the pretreatment of canbe used on line sample And analysis overall process.Its application purpose generated is to realize ultimate aim-chip lab of micro-total analysis system, at present work The focus on the application field for making to develop is life science.
Current international research present situation：Innovation focuses mostly in terms of separation, detection architecture；To how to introduce practical sample on chip The problems of product analysis, such as sample is introduced, is varied, the related research of pre-treatment is also very weak.Its development is dependent on more The development of subject crossing.
And NC films are common a kind of detection techniques in current biomedical inspection, which existed using analyte The characteristic that will produce fluorescence under the excitation of special wavelength light carries out qualitative and quantitative detection to it.Since fluorescence immunoassay detection technique has There are the series of advantages such as the fast, safety and stability of high sensitivity, high specificity, detection speed, therefore is widely used in clinical inspection In survey, in endocrine disease detection, infectious disease detection, obstetrical and gynecological disease detection, tumor-marker analyte detection, the disease inspection of hereditary section The detection fields such as survey, blood and cytology detection have broad application prospects.
In Chinese patent literature CN107843583A, a kind of micro-fluidic chemiluminescent analyzer and user are disclosed Method, including fluid path gas path module, chip load-on module, ultrasonic module, fluid path gas path module, chip load-on module, ultrasonic module Sequence is arranged.Present invention collection is multiple to be installed on one, can carry out barcode scanning to each chip, determine personalization detection project；Make So that sample, reaction reagent, luminous substrate is mixed well with ultrasonic module, finally carry out optical detection, reads data, make entirely to examine Program standardization, automation are surveyed, and detection sensitivity is high, result is reliable.The present invention on the basis of ensureing every detection function, Structure has carried out great simplification, and small volume, manufacturing cost is low, easy to operate, is suitble to carry out real-time test at one's side in patient, Testing result can be quickly obtained.
But above-mentioned technical proposal can not take into account the Technology application scene of micro-fluidic chip and the detection of NC films, micro-fluidic core Piece detection has precision high, and speed is fast, the higher feature of testing cost, is appropriate for the detection that accurate medical rings carry out, NC Film detection has speed fast, and precision is low, the low feature of testing cost, is generally used for the detection of screening type.Therefore, it is necessary to by this two The advantages of kind technology, is merged, and the actual conditions of R. concomitans scene are rationally detected, and reaches social benefit and economic effect The organic unity of benefit.
Invention content
The technical problem to be solved in the present invention is to provide a kind of Technology application fields for taking into account micro-fluidic chip and the detection of NC films Scape, meet micro-fluidic and NC films detect two kinds of measuring tables simultaneously sample introduction for micro-fluidic chip and the more flux detections of NC films one The device of body machine.
In order to solve the above technical problems, the technical solution adopted by the present invention is, it is mostly logical that this is used for micro-fluidic chip and NC films The device of amount detection all-in-one machine, includes pedestal, and sample introduction module is provided on the pedestal, incubates module, cleaning module, NC films Detection module and chip detection module；
Wherein, it is provided with mutually independent chip sample introduction card slot and NC film sample introduction card slots in the sample introduction module, uses respectively In insertion chip or NC film strips；Fluid path gas path module is additionally provided in the sample introduction module；
There is mutually independent chip to incubate slot and NC films in the incubation module and incubate slot, and is the sample introduction module, clear The side of mold cleaning block and NC films detection module on the incubation block length direction is arranged；It is cleaned in the cleaning module Chip afterwards send into the chip detection module and is detected.
Fluid path gas path module in sample introduction module, for ensuring that chip reaction warehouse is reacted full of sample and reagent；Chip temperature Slot is educated, for carrying out constant-temperature incubation to chip；Similarly, NC films incubate slot, for carrying out constant-temperature incubation to NC film strips；Using upper Technical solution is stated, modules component is incorporated on pedestal and forms complete equipment, it is structure-integrated, it can especially take into account The Technology application scene of micro-fluidic chip and the detection of NC films, micro-fluidic chip detection have precision high, and speed is fast, testing cost compared with High feature is appropriate for the detection that accurate medical rings carry out, and the detection of NC films has speed fast, and precision is low, and testing cost is low The characteristics of, it is generally used for the detection of screening type；It all can go to detect with high-precision methodology to avoid any project, lead to medical money It the advantages of the problem of overconsumption in source, technology that there are two types of tools, can be rationally detected with the actual conditions of R. concomitans scene, The organic unity for reaching Social benefit and economic benefit can use NC film lateral chromatographies for the less high project of sensitivity requirement Method solves, and can not only reduce cost, but also can improve flux；However and stability requirement especially high to sensitivity requirement is also very High, highly difficult project can be detected with microfluidic methods, you can improved sensitivity, also be can reach accuracy.Furthermore in this hair In bright technical solution, micro-fluidic chip and the detection of NC films are mutual indepedent, do not interfere with each other, can meet and meanwhile two kinds of measuring tables simultaneously Sample introduction meets the cooperation demand of two methods during the system detectio of POCT detection process, improves detection efficiency.
Preferably, the side of the chip sample introduction card slot is provided with chip sample introduction stirring hand, the chip sample introduction stirring hand is solid Surely it is connected on the first drive connection block, the first drive connection block passes through synchronization by the chip sample introduction stirring hand power motor Band driving moves back and forth；The side of the NC films sample introduction card slot is provided with NC film sample introduction stirring hands, the NC films sample introduction stirring hand is solid Surely it is connected on the second drive connection block, the second drive connection block passes through synchronization by the NC films sample introduction stirring hand power motor Band driving moves back and forth.
Chip is inserted into chip sample introduction card slot, chip sample introduction card slot enters inside the device of all-in-one machine of the present invention, arrives Up to position, i.e., chip is moved in the fluid path gas path module in sample introduction module, by fluid path gas path module to adding sample Chip is handled；Chip sample introduction stirring hand is arranged in the side of chip sample introduction card slot so that chip sample introduction stirring hand controls motor band Chip is dialled in chip incubation slot and carries out constant-temperature incubation by dynamic chip sample introduction stirring hand；NC film strips are inserted into NC films into scale In slot, NC film sample introduction card slots enter inside the device of all-in-one machine of the present invention, in-position, and NC film sample introduction stirring hand power motors drive NC film strips are dialled in NC films incubation slot and carry out constant-temperature incubation by NC film sample introduction stirring hands.
Preferably, the first transmission draw runner is provided below in the first drive connection block；Under the second drive connection block Face is provided with the second transmission draw runner；The chip sample introduction card slot controls motor control, the NC films sample introduction by chip sample introduction card slot Card slot controls motor control by NC film sample introduction card slots, and the fluid path gas path module is carried out by fluid path gas path module control motor control Action.
Drive connection block is moved back and forth along transmission draw runner, and position is accurate, mobile to stablize, and not will produce shaking；It adopts Motor-driven control chip provided sample introduction card slot is controlled with chip sample introduction card slot, motor control NC films are controlled into scale using NC film sample introduction card slots Slot keeps independence between the two, chip, NC film strips to enter more convenient, convenient inside the device of all-in-one machine of the present invention； Fluid path gas path module is pushed by fluid path gas path module control motor control, and then is handled chip.
Preferably, the fluid path gas path module includes upper sliding block, sample introduction gas circuit column, fluid path column, liquid level bar and valve Compression bar；The sample introduction gas circuit column end is provided with gas circuit sealing element, has sealing ring on the gas circuit sealing element；The valve pressure Bar controls motor control by valve, and the valve compression bar control motor and fluid path gas path module control motor are arranged at institute It states above fluid path gas path module.
The setting of upper sliding block, the direction and position pushed to chip for ensuring fluid path gas path module is accurate, into Sample gas circuit column is blown afloat for positive pressure pushes the sample being added in chip to reach reaction zone, gas circuit sealing element, for ensureing device Sealing reliability at (fluid path gas path module) and chip cooperation, using sealing ring (flexible, such as rubber material), integration Sealing means utmostly meet the sample introduction and cleaning seal request of micro-fluidic chip；Fluid path column is for clear in encapsulating chip Washing lotion mouth does not allow it ventilative；Whether liquid level bar reaches and for incuding sample full of reaction zone；Valve compression bar is used to work as liquid level bar Sense that sample reaches and be full of reaction zone, valve control motor makes closing valve response immediately, and valve compression bar kills Valve ensures that chip reaction warehouse is reacted full of sample and reagent；Using liquid level bar carry out liquid level sensing, can accurate sense it is micro-fluidic Chip interior liquid mobility status grasps the volume of sample and cleaning solution in micro-fluidic chip in time, and can automatic decision miniflow Control chip pipeline blockage situation.
Preferably, in the incubation module, the chip incubates slot and NC films incubate slot closed assembly setting up and down；The core Piece incubates slot, and there is at least one slot position to be used for chip placement, and the chip incubates slot and controls motor control by chip incubation slot, So that the chip incubation slot is free to slide along chip incubation slot polished rod；The NC films incubate slot, and there is at least one slot position to be used for NC film strips are placed, the NC films incubate slot and incubate slot control motor control by NC films so that the NC films incubate slot along NC films It is free to slide to incubate slot polished rod.
Chip incubates slot and NC films incubate slot closed assembly setting up and down, compact-sized, and embodies the independence of the two, not phase Interference；Chip incubation slot is free to slide along chip incubation slot polished rod, and sample introduction module, cleaning module and chip detection module are along institute The side setting incubated on block length direction is stated, facilitates and dials in the chip in sample introduction module in chip incubation slot, The chip for being also convenient for be located in chip incubation slot is dialled in cleaning module；Likewise, NC films incubate slot incubates slot along NC films Polished rod is free to slide, and the side of sample introduction module, cleaning module and NC films detection module on the incubation block length direction Setting facilitates and dials in NC film strips in NC films incubation slot, and the NC film strips that NC films incubate in slot will be located at by, which being also convenient for, push In NC film detection modules；Slot position can be arranged multiple, and multi-chip or more NC films strips synchronize detection, can meet multiple chips, more NC films strip Synchronous Transmission in systems, meets the needs of detecting system is to flux and efficiency.
Preferably, there is the cleaning module chip rinse bath, the chip rinse bath to clean drive connection block with chip It is fixedly connected, the synchronous belt that control motor driving is driven by chip rinse bath is fixedly mounted in the chip cleaning drive connection block On, the chip rinse bath moves back and forth in the linear guide；The cleaning module controls motor control by cleaning fluid path gas circuit, And the cleaning fluid path gas circuit control motor is arranged above the cleaning module.
In the present invention, cleaning module is identical as fluid path gas path module on concrete structure, and cleaning fluid path gas circuit controls horse It is pushed up to control cleaning module, cleaning module is ensured using structure design same with fluid path gas path module (gas circuit sealing element) Air-tightness is beaten cleaning solution using liquid pump positive pressure and is cleaned, and cleaning for several times, has beaten cleaning solution every time, is blown just using air pump control Body of calming the anger empties the cleaning solution in chip reaction zone, until cleaning is completed；Gone the same way mode using gas-liquid, using an injection port and Valve pump control system can meet specific demand of the micro-fluidic chip to gas-liquid sample introduction in conjunction with microcomputer control sequential.
Preferably, the NC films detection module includes NC film light paths, is provided with below the NC films light path NC film test card slots detect NC films light path described in control motor control by NC films and are moved above the NC films test card slot It is dynamic；The side of the NC films test card slot is provided with limited post, the NC film test card slots described in discarding strip control motor control It is mobile.
After NC films strip is pushed from NC films incubation slot in NC film test card slots, NC films detection control motor control NC films Light path is moved forward and backward acquisition signal above NC film test card slots, is analyzed, after detection, is controlled by discarding strip Motor control NC film test card slots move right, and the side of NC film test card slots is provided with limited post, and limitation NC films strip is to the right Mobile, when NC film test card slots are moved to certain position, NC film strips fall down NC film test card slots, and it is dynamic that strip is abandoned in completion Make.
Preferably, the NC films gone out in sample detection means by NC films go out sample stirring hand and NC films strip are incubated slot from the NC films In push in the NC films test card slot.
Preferably, the chip detection module includes excitation module, and sending and receiving are provided with below the excitation module Optical port is penetrated, by the driving barn door movement of barn door drive motor, the lower section that optical port is penetrated in the sending and receiving is arranged in the barn door；Position It is additionally provided with photomultiplier PMT in the bottom of the chip detection module；Barn door is set, prevents side light source to detection Interference, improves the sensitivity of optical detection.
Preferably, it is connected with turntable on the output shaft of the barn door drive motor, has on the turntable and stirs column, institute It states and stirs column and be inserted into the groove of the barn door.
By the rotation of turntable, drives and stir column movement on turntable so that barn door moves left and right.
Description of the drawings
It is further described below in conjunction with the accompanying drawings with embodiments of the present invention：
Fig. 1 is apparatus structure schematic diagram of the present invention for micro-fluidic chip and the more flux detection all-in-one machines of NC films；
Fig. 2 is the sample introduction module structural schematic diagram in Fig. 1；
Fig. 3 is the sample introduction module backsight structural representation in Fig. 2；
Fig. 4 is the fluid path gas path module structural schematic diagram in the present invention；
Fig. 5 is the gas circuit seal configuration schematic diagram in Fig. 4；
Fig. 6 is the incubation modular structure schematic diagram in Fig. 1；
Fig. 7 is the cleaning module structural schematic diagram in Fig. 1；
Fig. 8 is the NC film detection module structural schematic diagrams in Fig. 1；
Fig. 9 is the chip detection module structural schematic diagram in the present invention；
Wherein：1- pedestals；2- sample introduction modules；201- chip sample introduction card slots；202-NC film sample introduction card slots；203- chip sample introductions Stirring hand；204- the first drive connection blocks；205- chip sample introduction stirring hand power motors；206-NC film sample introduction stirring hands；207- second is passed Dynamic link block；208-NC film sample introduction stirring hand power motors；209- first is driven draw runner；2010- second is driven draw runner；2011- cores Piece sample introduction card slot controls motor；2012-NC film sample introduction card slots control motor；3- incubates module；301- chips incubate slot；302-NC Film incubates slot；303-NC films incubate slot and control motor；304- chips incubate slot and control motor；305- chips incubate slot polished rod； 306-NC films incubate slot polished rod；307- driving blocks；308- holders；309- skids off mouth；4- cleaning modules；401- chips clean Slot；402- chips clean drive connection block；403- chip rinse baths transmission control motor；404- the linear guides；405- cleaning solutions Road gas circuit controls motor；5-NC film detection modules；501-NC film light paths；502-NC film test card slots；503-NC films detect Control motor；504- limited posts；505- abandons strip and controls motor；506- detection platforms；507- keeps out piece；508-NC film strips Rest area；509-NC film strips fall into mouth；5010- surrounding edges；5011- limits post holes；5012-NC films detect sliding block；6- chips are examined Survey module；601- excitation modules；Optical port is penetrated in 602- sending and receiving；603- barn door drive motors；604- barn doors；605- photoelectricity times Increase pipe PMT；606- output shafts；607- turntables；608- stirs column；609- grooves；7- fluid path gas path modules；701- fluid path gas circuit moulds Block controls motor；The upper sliding blocks of 702-；703- sample introduction gas circuit columns；704- fluid path columns；705- liquid level bars；706- valve compression bars； 707- gas circuit sealing elements；708- sealing rings；709- valves control motor；7010- upper brackets；7011- lower brackets；7012、7018- Feed screw nut；The upper surfaces 7013- layer；The lower faces 7014- layer；7015- notches；7016,7017- lead screws；8-NC films go out sample detection Component；801-NC films go out sample stirring hand.
Specific implementation mode
As shown in Figure 1, being that the apparatus structure for micro-fluidic chip and the more flux detection all-in-one machines of NC films of the invention shows It is intended to, includes pedestal 1, sample introduction module 2 is provided on the pedestal 1, incubates module 3, cleaning module 4, NC films detection module 5 With chip detection module 6；
Wherein, mutually independent chip sample introduction card slot 201 and NC film sample introductions card slot 202 are provided in the sample introduction module 2, It is respectively for insertion into chip or NC film strips；Fluid path gas path module 7 is additionally provided in the sample introduction module 2；
There is mutually independent chip to incubate slot 301 and NC films incubation slot 302, and the sample introduction in the incubation module 3 The side of module 2, cleaning module 4 and NC films detection module 5 on incubation 3 length direction of module is arranged；In the cleaning Chip after being cleaned in module 4, which is sent into the chip detection module 6, to be detected.
As shown in Figure 2,3, the side of the chip sample introduction card slot 201 is provided with chip sample introduction stirring hand 203, the chip Sample introduction stirring hand 203 is fixedly connected on the first drive connection block 204, and the first drive connection block 204 is by the chip sample introduction Stirring hand power motor 205 is moved back and forth by synchronous belt driving；The side of the NC films sample introduction card slot 202 be provided with NC films into Sample stirring hand 206, the NC films sample introduction stirring hand 206 are fixedly connected on the second drive connection block 207, the second drive connection block 207 are moved back and forth by the NC films sample introduction stirring hand power motor 208 by synchronous belt driving.
The first transmission draw runner 209 is provided below in the first drive connection block 204；The second drive connection block 207 The second transmission draw runner 2010 is provided below；The chip sample introduction card slot 201 is controlled by chip sample introduction card slot control motor 2011, The NC films sample introduction card slot 202 is controlled by NC film sample introduction card slots control motor 208, and the fluid path gas path module 7 is by fluid path gas circuit The module control control of motor 701 is acted.
As shown in figure 4, the fluid path gas path module 7 includes upper sliding block 702, sample introduction gas circuit column 703, fluid path column 704, Liquid level bar 705 and valve compression bar 706；The end set of the sample introduction gas circuit column 703 has gas circuit sealing element 707, the gas circuit close There is sealing ring 708 (as shown in Figure 5), in the present embodiment, the material of sealing ring 708 is rubber, realizes soft connect on sealing 707 It touches, uses multiple tracks muscle bridge structure, muscle bridge to be squeezed deformation on sealing ring semi arch, start sealing function；The valve compression bar 706 are controlled by valve control motor 709, and the valve compression bar control motor 709 and the fluid path gas path module control motor 701 It is arranged at 7 top of the fluid path gas path module.
In the present embodiment, the end of fluid path column 704 is also provided with gas circuit sealing element 707, on gas circuit sealing element 707 Sealing ring 708 is installed；In addition, with regard to the fluid path gas path module 7 of the present embodiment, specifically, also upper bracket 7010, valve compression bar Control motor 709 and fluid path gas path module control motor 701 are arranged on upper bracket 7010, and lower bracket 7011 passes through upper downslide Block 702 is flexibly connected with upper bracket 7010, wherein sample introduction gas circuit column 703, fluid path column 704 and liquid level bar 705 are installed in lower branch On frame 7011, it is lead screw motor that fluid path gas path module, which controls motor 701, with the lead screw on fluid path gas path module control motor 701 The equipped feed screw nut 7012 of 7016 phases is fixedly mounted on lower bracket 7011, and driving lower bracket 7011 slides up and down, lower bracket It is divided into two layers, is upper surface layer 7013 and lower face layer 7014 respectively, upper surface layer 7013 is used to install feed screw nut 7012, with And also there are one notches 7015 for tool on upper surface layer 7013, give the equipped silk of 7017 phase of lead screw of valve control motor 709 Stem nut 7018 reserves installation site, and valve compression bar 706 is actively plugged in lower face layer 7014, and fluid path column 704 is also associated with Liquid pump, sample introduction gas circuit column 703 are also associated with air pump.
In the incubation module 3, as shown in fig. 6, the chip incubates slot 301 and NC films incubate about 302 closed assembly of slot Setting；The chip incubates slot 301, and there is at least one slot position to be used for chip placement (in the present embodiment, having 8 slot positions), The chip incubates slot 301 and is controlled by chip incubation slot control motor 304 so that the chip incubates slot 301 and incubated along chip Slot polished rod 305 is free to slide；The NC films, which incubate slot 302, has at least one slot position for placing NC film strips (in this implementation In example, and there are 8 slot positions), the NC films incubate slot 302 and are controlled by NC films incubation slot control motor 303 so that the NC It is free to slide along NC films incubation slot polished rod 306 that film incubates slot 302.
In incubating module 3, there is holder 308, NC films to incubate slot 302 and be located above, there are two NC films to incubate slot light Bar 306, there are synchronous belt installation space between two NC films incubate slot polished rod 306, NC films incubate slot control motor 303 and connect There are synchronous belt, NC films to incubate slot 302 and driving block 307 is provided below, driving block 307 incubates slot with NC films and controls motor The synchronous belt of 303 drivings is fixedly connected；Chip incubates slot 301 and is located at the lower section that NC films incubate slot 302, structure and above-mentioned NC films temperature It educates that slot 302 is identical, in addition, on the right side of holder 308, and with chip incubates the corresponding position of slot 301 and be provided with and skid off mouth 309, The chip that chip is incubated to incubation time completion in slot 301 is facilitated to be allocated in chip rinse bath 401.
Cleaning module 4 has chip rinse bath 401, the chip rinse bath 401 and chip cleaning drive connection block 402 solid Fixed connection, the chip cleaning drive connection block 402 be fixedly mounted on by chip rinse bath be driven control motor 403 drive it is same Step takes, and the chip rinse bath 401 moves back and forth in the linear guide 404；The cleaning module 4 is by cleaning fluid path gas circuit control Motor 405 processed controls, and cleaning fluid path gas circuit control motor 405 is arranged above the cleaning module 4, as shown in Figure 7.
As shown in Fig. 8 the NC films detection module 5 of the present invention, includes NC films light path 501, the NC films detection 501 lower section of light path is provided with NC films test card slot 502, and detecting control motor 503 by NC films controls the NC films light path 501 is mobile above the NC films test card slot 502；The side of the NC films test card slot 502 is provided with limited post 504, by It abandons strip control motor 505 and controls the movement of NC films test card slot 502.
Specifically, NC films detection module 5 includes detection platform 506, and the edge of detection platform 506, which is provided with, keeps out piece 507, It keeps out piece 507 and is surrounded by NC film strips rest area 508 with NC films test card slot 502；Below NC film strips rest area 508, and It is provided with NC film strips in detection platform 506 and falls into mouth 509；Surrounding edge 5010 in NC films test card slot 502 has limit Post holes 5011；NC films light path 501 is fixedly connected with NC films detection sliding block 5012, and NC films detection sliding block 5012 is detected by NC films The control movement of motor 503 is controlled, and then drives NC films light path 501 mobile above NC films test card slot 502.
In Fig. 1, we can further be seen that the NC films gone out in sample detection means 8 by NC films go out sample stirring hand 801 by NC films Strip is incubated in slot 302 from the NC films and is pushed in the NC films test card slot 502.
Shown in Fig. 9 is chip detection module 6, includes excitation module 601, and 601 lower section of the excitation module is set It is equipped with sending and receiving and penetrates optical port 602, drive barn door 604 to move by barn door drive motor 603, the barn door 604 is arranged in institute The lower section that optical port 602 is penetrated in sending and receiving is stated, different optical filters can be installed on barn door 604, barn door controls motor 603 and moves shading Plate 604 carries out different optical wavelength and receives；It is additionally provided with photomultiplier positioned at the bottom of the chip detection module 6 PMT605, chip is placed on excitation module 601 and sending and receiving are penetrated between optical port 602；The output shaft of barn door drive motor 603 It is connected with turntable 607 on 606, has on the turntable 607 and stirs column 608, the column 608 of stirring is inserted into the barn door In 604 groove 609.
When specifically used：
(1) NC film strips are detected：NC film strips are inserted into NC film sample introductions card slot 202, NC film sample introduction card slots It controls the control NC film sample introductions of motor 2012 card slot 202 to enter inside device, reaches precalculated position, NC film sample introduction stirring hand power motors NC film strips are dialled in NC films incubation slot 302 and carry out constant-temperature incubation by 208 drive NC film sample introductions stirring hands 206, and incubation time is complete At NC films incubate slot control motor 303 and NC films incubation slot 302 is driven to move to designated position, and NC films go out sample stirring hand 801 by NC films Strip is pushed from the slot position that NC films incubate slot 302 in the NC films test card slot 502 in NC films detection module 5, the detection control of NC films Motor 503 processed controls NC films light path 501 and is moved forward and backward acquisition signal, is analyzed, and after detection, abandons strip control Motor 505 controls NC films test card slot 502 and moves right, and limited post 504 passes through in the surrounding edge 5010 of NC films test card slot 502 Post holes 5011 is limited, limitation NC film strips move right, when NC films test card slot 502 continues to move to certain position, NC films Strip falls down NC films test card slot 502 from NC film strips rest area 508, falls into mouth 509 into NC film strips, NC films are abandoned in completion Strip acts；
(2) micro-fluidic chip is detected：The chip that sample has been added is inserted into chip sample introduction card slot 201, core Piece sample introduction card slot controls the control chip sample introduction of motor 2011 card slot 201 and enters inside device, reaches precalculated position, fluid path gas circuit mould Block controls motor 701 and controls 7 pushing of fluid path gas path module, ensures the sealing at device (fluid path gas path module 7) and chip cooperation Reliability, fluid path column 704 seal the cleaning solution mouth on chip, and sample introduction gas circuit column 703 pushes down the injection port on chip, then, into Sample gas circuit column 703 injection port positive pressure blow afloat push sample reach reaction zone, at this moment, liquid level bar 705 sense sample reach and Full of reaction zone, valve control motor 709 makes closing valve response immediately, and valve compression bar 706 kills valve, ensures core Piece reaction warehouse is reacted full of sample and reagent；Positioned at 2 bottom of sample introduction module ultrasonic module (existing) upwards contact chip into Row ultrasound mixing, ensures the abundant reaction of reagent and sample；Chip sample introduction stirring hand power motor 205 drives chip sample introduction stirring hand Chip is dialled in chip by 203 to be incubated in slot 301 and carries out constant-temperature incubation, and incubation time is completed；Chip incubates slot and controls motor 304 It drives chip to incubate slot 301 and moves to designated position, chip is pushed in chip rinse bath 401, the transmission control of chip rinse bath Motor 403 moves to indirect control chip rinse bath 401, is moved to immediately below cleaning liquid circuit module, cleans fluid path gas circuit control Motor 405 processed controls cleaning module 4 and pushes, and cleaning module 4 ensures gas using with 7 same structure design of fluid path gas path module Close property, then, liquid pump positive pressure is beaten cleaning solution and is cleaned, and cleaning for several times, has beaten cleaning solution every time, and barotropic gas is blown in air pump control The cleaning solution in chip reaction zone is emptied, cleaning is completed, and chip rinse bath 401 moves backward to chip detection module 6 and carries out light Road acquisition process (chip is placed on excitation module 601 and sending and receiving are penetrated between optical port 602), after data acquire, by chip It loses.
Above-mentioned two detecting step can be carried out individually, can also be carried out at the same time；In the present embodiment, it can also use micro- Fluidic chip testing process monitoring system, the real-time reaction process of real time monitoring micro-fluidic chip and detection time, meet and use Person understands detection progress, and system can enter instrument and start first time of detection in chip distributes the detection management shelves of chip automatically Case knows chip project and parameter curve by chip two dimensional code, thus display chip time of measuring and remaining detection time, User is facilitated to grasp detection procedure in real time；Automatically the consumptive material that will be generated in NC films and micro-fluidic chip detection process separately abandons, It stores classifiedly；Dual area sample mode improves accuracy of detection and stability, cleverly meet micro-fluidic chip to sampling precision and The requirement of stability.
The basic principles and main features and advantage of the present invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, such as being mounted on Some adjustment are done in the position of all parts on pedestal, and these changes and improvements all fall within the protetion scope of the claimed invention.This Invention is claimed range and is defined by the appending claims and its equivalent thereof.

Claims (10)

1. a kind of device for micro-fluidic chip and the more flux detection all-in-one machines of NC films includes pedestal, which is characterized in that institute It states and is provided with sample introduction module on pedestal, incubates module, cleaning module, NC films detection module and chip detection module；
Wherein, it is provided with mutually independent chip sample introduction card slot and NC film sample introduction card slots in the sample introduction module, is respectively used to insert Enter chip or NC film strips；Fluid path gas path module is additionally provided in the sample introduction module；
There is mutually independent chip to incubate slot and NC films incubation slot, and the sample introduction module, cleaning mould in the incubation module The side of block and NC films detection module on the incubation block length direction is arranged；After being cleaned in the cleaning module Chip send into the chip detection module and is detected.
2. the device according to claim 1 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In being provided with chip sample introduction stirring hand in the side of the chip sample introduction card slot, the chip sample introduction stirring hand is fixedly connected on first On drive connection block, the first drive connection block is backhauled by the chip sample introduction stirring hand power motor by synchronous belt driving It is dynamic；The side of the NC films sample introduction card slot is provided with NC film sample introduction stirring hands, the NC films sample introduction stirring hand is fixedly connected on second On drive connection block, the second drive connection block is backhauled by the NC films sample introduction stirring hand power motor by synchronous belt driving It is dynamic.
3. the device according to claim 2 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In the first transmission draw runner is provided below in the first drive connection block；The second drive connection block is provided below second It is driven draw runner；The chip sample introduction card slot by chip sample introduction card slot control motor control, the NC films sample introduction card slot by NC films into Scale slot controls motor control, and the fluid path gas path module is acted by fluid path gas path module control motor control.
4. the device according to claim 3 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In the fluid path gas path module includes upper sliding block, sample introduction gas circuit column, fluid path column, liquid level bar and valve compression bar；The sample introduction Gas circuit column end is provided with gas circuit sealing element, has sealing ring on the gas circuit sealing element；The valve compression bar is controlled by valve Motor control, the valve compression bar control motor and fluid path gas path module control motor are arranged at the fluid path gas circuit mould Above block.
5. the device according to claim 1 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In in the incubation module, the chip incubates slot and NC films incubate slot closed assembly setting up and down；The chip, which incubates slot, to be had At least one slot position is used for chip placement, and the chip incubates slot and incubates slot control motor control by chip so that the chip It is free to slide along chip incubation slot polished rod to incubate slot；The NC films, which incubate slot, has at least one slot position for placing NC film cards Item, the NC films incubate slot and incubate slot control motor control by NC films so that the NC films incubate slot and incubate slot polished rod along NC films It is free to slide.
6. the device according to claim 1 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In the cleaning module has chip rinse bath, and the chip rinse bath is fixedly connected with chip cleaning drive connection block, described Chip cleans drive connection block and is fixedly mounted on the synchronous belt by the transmission control motor driving of chip rinse bath, the chip cleaning Slot moves back and forth in the linear guide；The cleaning module controls motor control, and the cleaning fluid path by cleaning fluid path gas circuit Gas circuit controls motor and is arranged above the cleaning module.
7. the device according to claim 1 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In, the NC films detection module includes NC film light paths, and NC film test card slots are provided with below the NC films light path, NC films light path described in control motor control is detected by NC films to move above the NC films test card slot；The NC films inspection The side for surveying card slot is provided with limited post, and NC film test card slots move described in discarding strip control motor control.
8. the device according to claim 7 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In the NC films gone out in sample detection means by NC films go out sample stirring hand and NC films strip is pushed the NC from NC films incubation slot In film test card slot.
9. the device according to claim 1 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In the chip detection module includes excitation module, and being provided with sending and receiving below the excitation module penetrates optical port, by shading Plate drive motor drives barn door movement, the barn door that the lower section for penetrating optical port in the sending and receiving is arranged；It is examined positioned at the chip The bottom for surveying module is additionally provided with photomultiplier PMT.
10. the device according to claim 9 for micro-fluidic chip and the more flux detection all-in-one machines of NC films, feature exists In, it is connected with turntable on the output shaft of the barn door drive motor, has on the turntable and stirs column, it is described to stir column insertion To the groove of the barn door.
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