CN111487400A - Time-resolved fluorescence immunoassay analyzer - Google Patents
Time-resolved fluorescence immunoassay analyzer Download PDFInfo
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- CN111487400A CN111487400A CN202010444390.7A CN202010444390A CN111487400A CN 111487400 A CN111487400 A CN 111487400A CN 202010444390 A CN202010444390 A CN 202010444390A CN 111487400 A CN111487400 A CN 111487400A
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- 238000011534 incubation Methods 0.000 claims abstract description 118
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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Abstract
The invention discloses a time-resolved fluoroimmunoassay analyzer, which comprises a base, a sample introduction module, an incubation module, a cleaning module and a chip detection module, wherein the sample introduction module is used for introducing a sample to be analyzed; the sample injection module is internally provided with a chip loading module and a liquid path and gas path module, the chip loading module is used for carrying a chip to the liquid path and gas path module, and the liquid path and gas path module is used for enabling a sample in the chip to flow to a preset position; thereafter the chip is transported into the incubation module; the incubation module enables the chip to react at a constant temperature within a set temperature; the incubation module freely moves up and down along the vertical direction, and the sample introduction module, the cleaning module and the chip detection module are respectively arranged on the side surface of the incubation module in the vertical movement direction; the chip cleaned in the cleaning module is sent to a chip detection module by the incubation module for detection. The modules are arranged in the vertical direction, so that the whole structure is compact, and the occupied area is small; and the function coordination of each module is smooth and simple, and the detection efficiency is high.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to a time-resolved fluoroimmunoassay analyzer for fluoroimmunoassay based on a microfluidic chip project.
Background
Microfluidic chips (microfluidic chips) are a hot spot area for the development of current micro total analysis Systems (minidesigned total analysis Systems). The micro-fluidic chip analysis takes a chip as an operation platform, simultaneously takes analytical chemistry as a basis, takes a micro-electromechanical processing technology as a support, takes a micro-pipeline network as a structural characteristic, takes life science as a main application object at present, and is the key point of the development of the field of the current micro total analysis system. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip. The micro-fluidic chip is a main platform for realizing the micro-fluidic technology. The device is characterized in that the effective structure (channels, reaction chambers and other functional parts) for containing the fluid is at least in one latitude in micron scale. Due to the micro-scale structure, the fluid exhibits and develops specific properties therein that differ from those of the macro-scale. Thus developing unique assay-generated properties. The characteristics and development advantages of the micro-fluidic chip are as follows: the micro-fluidic chip has the characteristics of controllable liquid flow, extremely less consumption of samples and reagents, ten-fold or hundred-fold improvement of analysis speed and the like, can simultaneously analyze hundreds of samples in a few minutes or even shorter time, and can realize the whole processes of pretreatment and analysis of the samples on line. The application of the micro total analysis system aims to realize the ultimate goal of the micro total analysis system, namely a lab-on-a-chip, and the key application field of the current work development is the field of life science.
Current state of international research: the innovation is mostly focused on the aspects of a separation and detection system; many problems of how to introduce actual sample analysis on a chip, such as sample introduction, sample change, pretreatment, and the like, are still very weak. Its development relies on the development of multidisciplinary crosses.
In chinese patent document CN108414522A, a device for a microfluidic chip and NC membrane multi-flux detection all-in-one machine is disclosed, which comprises a base, wherein a sample introduction module, an incubation module, a cleaning module, an NC membrane detection module and a chip detection module are arranged on the base; the sample injection module is internally provided with a chip sample injection clamping groove and an NC membrane sample injection clamping groove which are mutually independent and are respectively used for inserting a chip or an NC membrane clamping strip; a liquid path and gas path module is also arranged in the sample injection module; the incubation module is provided with a chip incubation groove and an NC membrane incubation groove which are mutually independent, and the sample introduction module, the cleaning module and the NC membrane detection module are arranged along the side surface of the incubation module in the length direction; and conveying the cleaned chip in the cleaning module to the chip detection module for detection.
However, in the technical scheme, each part is arranged along the side surface of the incubation module in the length direction, and is laid and unfolded, so that too much space is occupied, and the equipment is too bulky in the use process; in addition, the connection between the modules needs to be further improved, and the matching is not very smooth.
Disclosure of Invention
The invention aims to provide a time-resolved fluoroimmunoassay analyzer which has a compact overall structure, occupies a small area, adopts vertical arrangement and is smooth and simple in functional coordination of modules.
In order to solve the technical problem, the invention adopts the technical scheme that the time-resolved fluoroimmunoassay analyzer comprises a base, a sample introduction module, an incubation module, a cleaning module and a chip detection module;
the sample injection module is internally provided with a chip loading module and a liquid path and gas path module, the chip loading module is used for carrying a chip to the liquid path and gas path module, and the liquid path and gas path module is used for enabling a sample in the chip to flow to a preset position; thereafter the chip is transported into the incubation module;
the incubation module enables the chip to react at constant temperature within a set temperature;
the incubation module freely moves up and down along the vertical direction, and the sample introduction module, the cleaning module and the chip detection module are respectively arranged on the side surface of the incubation module in the vertical movement direction; and conveying the chip cleaned in the cleaning module to the chip detection module for detection by the incubation module.
The liquid path and gas path module in the sample introduction module is used for enabling a sample in the chip to flow to a preset position and ensuring that the reaction cavity of the chip is filled with the sample and a reagent for reaction; by adopting the technical scheme, all module components are integrated on the base and are respectively arranged on the side surface of the incubation module in the vertical movement direction to form a whole set of time-resolved fluorescence immunoassay analyzer equipment, the structure is integrated, the whole structure is compact, the occupied area is small, the vertical arrangement is adopted, the waste of space caused by the spreading and unfolding of the equipment is avoided, and the function matching of all modules in the technical scheme is smooth and simple; the sample feeding module, the cleaning module and the chip detection module are all arranged around the incubation module, and the turnover of the chip is carried out by taking the incubation module as a center.
In the specific operation: placing a chip to be detected into an analyzer, and then moving the chip to a liquid path and gas path module under the driving of a chip loading module, so that a sample in a chip sample injection cavity slowly advances along a chip micro-channel and flows to a preset position; the chip is stirred to enable the chip to reach the incubation module, so that the chip reaction cavity performs sample immunoreaction at a preset temperature; after the reaction, stir the chip and wash the module, wash the module and can wash the chip after the reaction, the chip is washd and is accomplished, stirs the chip immediately and arrives the incubation module, sends the chip to the chip detection module by the incubation module and detects in, the incubation module rises to the chip detection module and corresponds the position. The detection result can be displayed on an instrument display screen, and the detection result can be printed by a built-in printer.
In a further development of the invention, the incubation module has an incubation well assembly comprising a heat patch and a heat retention patch; a plurality of stacked incubators in the incubator assembly, the plurality of stacked incubators being free to move up and down along the incubator slide. The incubation tank component is provided with a heating plate and a heat preservation plate so that the chip can react at a constant temperature within a set temperature.
The invention is further improved in that the incubation tank assembly is also provided with an incubation tank connecting block; the incubation groove connecting block is connected with an incubation groove moving belt, the band-type brake motor drives the incubation groove moving driving wheel, the incubation groove moving belt drives the incubation groove moving driven wheel to rotate, and therefore the multiple stacked incubation grooves in the incubation groove assembly can move up and down freely along the incubation groove sliding rail.
The invention further improves the structure that the incubation tank slide rail is arranged on an incubation tank slide rail bracket, a buffer block is arranged on the side surface of the incubation tank assembly, and correspondingly, a buffer cushion is arranged at the bottom of the incubation tank slide rail bracket; the incubator is characterized in that a limiting separation blade is arranged on the incubator assembly, a limiting optical coupler connecting block is fixedly connected to the incubator slide rail support, and a limiting optical coupler is mounted on the limiting optical coupler connecting block and used for limiting the up-and-down movement of the incubator. The side of incubation groove subassembly is provided with the buffer block, and the incubation groove slide rail support bottom that corresponds sets up the blotter, provides the buffering for the incubation groove downstream, prevents the damage, is provided with spacing separation blade on the incubation groove subassembly simultaneously, and incubation groove slide rail support fixedly connected with spacing opto-coupler connecting block, spacing opto-coupler are installed on spacing opto-coupler connecting block for the incubation groove up-and-down motion is spacing.
Preferably, the incubation groove is provided with a chip pressing strip, and two protrusions of the chip pressing strip are matched with grooves in the chip for positioning the incubation reaction on the chip.
Preferably, the heating plate and the warming plate are both disposed at the side of the plurality of stacked incubators.
The invention is further improved in that the chip loading module comprises a chip sample introduction handle, and the chip sample introduction handle pushes the chip to be put in place in a chip sample introduction groove; a chip limiting piece structure is arranged behind the chip sample feeding groove; the chip is driven by the chip sample feeding groove to move.
Preferably, the chip sample feeding hand lever is controlled by a chip sample feeding hand lever motion assembly to act; the chip sample injection hand shifting motion assembly comprises a sample injection hand shifting horizontal motion assembly and a sample injection hand shifting up-and-down motion assembly; advance kind and dial hand horizontal movement subassembly and have advance kind and dial hand horizontal movement slider, advance kind and dial hand horizontal movement slider along advance kind and dial hand horizontal sliding rail motion, it advances kind and dials hand horizontal movement slider connecting block to connect on the hand horizontal movement slider to advance kind, advance fixedly connected with chip advance kind and dial hand up-and-down motion slide rail on the hand horizontal movement slider connecting block, it advances kind and dials hand up-and-down motion slider to be connected with on the hand up-and-down motion slide rail to advance kind on the chip advance kind, advance kind and dial hand up-and-down motion slider with the chip advances kind and dial the hand and connect, it advances kind and dial the hand slide bar to be provided with the chip on the hand to advance kind to the chip, the chip advances.
Preferably, the chip advance the sample cell under advance the sample cell motor control, advance the sample cell motor nut along advancing the sample cell lead screw motion, advance the sample cell motor nut and advance a sample cell connecting block one and be connected, advance a sample cell connecting block one with the chip advances the sample cell and connect, advance a sample cell connecting block two with advance a sample cell connecting block one and connect, advance a sample cell connecting block two and advance a sample cell slider and be connected, advance a sample cell slider along advance a sample cell slide rail reciprocating motion.
Preferably, two sample feeding groove slide rails are arranged on the partition plate of the chip loading module, namely a sample feeding groove slide rail I and a sample feeding groove slide rail II; correspondingly, the number of the sample feeding groove sliding blocks is two, namely a first sample feeding groove sliding block and a second sample feeding groove sliding block, and the number of the second sample feeding groove connecting blocks is two, namely a first sample feeding groove connecting block and a second sample feeding groove connecting block; the first sample injection groove connecting block II is connected with the first sample injection groove sliding block I, the first sample injection groove sliding block I moves in a reciprocating mode along the first sample injection groove sliding rail, the second sample injection groove connecting block II is connected with the second sample injection groove sliding block II, and the second sample injection groove sliding block II moves in a reciprocating mode along the second sample injection groove sliding rail.
Preferably, the partition plate is provided with a first chip loading module support and a second chip loading module support, wherein the second chip loading module support is provided with a chip limiting piece sliding groove; the chip limiting piece structure is provided with a chip limiting piece, and a chip limiting piece sliding rod used for moving in the chip limiting sliding groove is arranged on the chip limiting piece; the chip advances a spacing slide rail of fixedly connected with chip on the lateral wall of appearance groove, be connected with the spacing piece slider of chip on the spacing piece slide rail of chip, the spacing piece of chip is connected on the spacing piece slider of chip, the spacing piece of chip is followed the appearance groove horizontal motion of chip, cooperates simultaneously spacing piece spout up-and-down motion of chip. The sliding groove of the first chip loading module support in the technical scheme has no specific function, and is convenient to process and manufacture in order to be uniform in structure with the second chip loading module support.
Preferably, the front end of the sample injection hand-shifting chute is higher than the rear end, so that the sample injection hand-shifting chute has a high starting point; advance kind and dial hand horizontal movement subassembly has advance kind and dial hand horizontal motor, advances kind and dial hand belt connecting block one end fixed connection advance kind on the horizontal belt of hand, advance kind dial hand belt connecting block the other end with advance kind and dial hand horizontal movement slider connecting block fixed connection.
Preferably, advance kind and dial hand horizontal movement subassembly still including advance kind and dial hand motion action wheel and advance kind and dial hand motion from the driving wheel, advance kind and dial hand horizontal belt cup joint advance kind and dial hand motion action wheel with advance kind and dial hand motion from the driving wheel on, advance kind and dial hand action wheel with advance kind and dial hand horizontal motor and be connected, advance kind and dial hand horizontal movement slider connecting block cup joint advance kind and dial on the hand horizontal belt. Advance kind and dial a hand horizontal motor start, the chip advances kind and dials a hand along advancing kind and dial a hand horizontal slide rail motion, advance kind and dial the initial position of hand in chip advance kind groove front end top (advance kind and dial a hand high starting point of hand spout), the chip is put into the back, the chip inserts the inductor sensing behind the chip, advance kind and dial the hand along advancing kind and dial a hand spout motion, the front end that will advance kind and dial a hand spout sets up to being higher than the rear end, the purpose is that not block the chip and put into chip advance kind groove, advance kind and dial the hand and dial the chip and make the chip arrive the advance kind groove completely and predetermine the position, prevent.
The invention is further improved in that the liquid path and gas path module comprises a liquid path and gas path control motor and a chip valve control motor; the liquid path gas circuit control motor drives the annular support to move downwards, the annular support is pressed downwards to enable a sample inlet interface on the annular support to seal a sample inlet cavity of the butt chip through a sample inlet sealing head, and an external gas-liquid port interface seals an external gas-liquid port of the butt chip through an external gas-liquid port sealing head; a liquid detection rod is fixedly connected to the annular support and is connected with a liquid detection sensor; the chip valve control motor drives the chip valve sliding block connecting block to move, a chip valve pressing rod is mounted on the chip valve sliding block connecting block, and the chip valve pressing rod presses down a valve pressing the chip under the control of the chip valve control motor.
Preferably, the liquid path and gas path module further comprises a liquid path and gas path slide rail and a liquid path and gas path slide block; the liquid path and gas path control motor is started, the liquid path and gas path lead screw rotates, a liquid path and gas path motor nut on the liquid path and gas path lead screw drives the annular support to move downwards, the annular support is connected with the liquid path and gas path slide rail and moves in a matched mode with the liquid path and gas path slide block, and the liquid path and gas path slide block is fixedly connected to the inner side of the side support of the liquid path and gas path module.
Preferably, the liquid path and gas path module further comprises a chip valve slide rail and a chip valve slide block; the chip valve control motor starts, and the chip valve lead screw is rotatory, chip valve motor nut on the chip valve lead screw drives chip valve slider connecting block motion, chip valve slider connecting block with chip valve slider connects, chip valve slide rail is installed the extension board inboard of liquid way gas circuit module. The liquid path and gas path control motor is started, the liquid path and gas path lead screw rotates, the liquid path and gas path motor nut drives the annular bracket to move downwards, the annular bracket is connected with the liquid path and gas path slide rail and moves in a matching way with the liquid path and gas path slide block, the liquid path and gas path slide block is fixedly connected to the inner side of the side bracket, the annular bracket is pressed downwards to enable a sample inlet interface (air inlet and pressurization effect) on the annular bracket to seal a sample inlet cavity of the butt joint chip through a sample inlet sealing head, an external gas and liquid port interface (sealing treatment, gas and liquid can not be fed) seals an external gas and liquid port of the butt joint chip through the external gas and liquid port sealing head, then, the sample in the sample inlet cavity of the chip slowly advances along a chip micro-flow passage through the gas inlet interface for pressurization, a liquid detection rod is fixedly connected to the annular bracket and connected with a, the liquid detection pole sensing is to the capacitance change, the chip valve control motor of instrument starts, chip valve lead screw is rotatory, chip valve motor nut drives the motion of chip valve slider connecting block, chip valve slider connecting block is connected with the valve slider, chip valve slide rail is installed in the extension board inboard, install chip valve depression bar on the chip valve slider connecting block, the valve is pushed down to chip valve depression bar under the control of chip valve control motor, make chip liquid can not continue to advance, the introduction port interface stops the pressurization of admitting air simultaneously.
Preferably, a chip valve limiting optocoupler and a chip valve limiting baffle are further arranged in the liquid path and gas path module and used for limiting the descending height of the chip valve compression bar; and the liquid path and gas path module is also provided with a liquid path and gas path limiting optocoupler and a liquid path and gas path limiting baffle for limiting the descending height of the annular support.
Preferably, the liquid path and gas path module further comprises a chip code scanner for scanning the two-dimensional code on the chip. Chip bar code scanner is located the top of chip, through it is right to advance the through-hole on the roof of kind module the chip bar code is swept, confirms whether chip testing item is correct, whether the chip is in the validity period, if not conform to the requirement then the machine reports to the police, suggestion error message.
The invention is further improved in that the cleaning module comprises a U-shaped shifting handle component and a cleaning module gas-liquid path component; after the incubation reaction of the chip is finished, the U-shaped hand-shifting in the U-shaped hand-shifting assembly shifts the chip to a cleaning position in the cleaning module gas-liquid path assembly for cleaning; and after the chip is cleaned, the U-shaped poking hand re-pokes the chip.
Preferably, the U-shaped shifting handle component is provided with a U-shaped shifting handle control motor, under the control of the U-shaped shifting handle control motor, the U-shaped shifting handle driving wheel rotates, the U-shaped shifting handle is driven by a U-shaped shifting handle belt, a U-shaped shifting handle belt connecting block on the U-shaped shifting handle belt is connected with the U-shaped shifting handle sliding block component, and the U-shaped shifting handle sliding block component is connected with the U-shaped shifting handle and moves along a U-shaped shifting handle sliding rail.
Preferably, a U-shaped shifting arm I and a U-shaped shifting arm II extend from the two sides of the U-shaped shifting hand respectively, and the distance between the U-shaped shifting arm I and the U-shaped shifting arm II is larger than the length of the chip.
Preferably, the cleaning module gas-liquid path assembly is provided with a cleaning module control motor, the cleaning module control motor is started, a cleaning module screw rod rotates, and a cleaning module motor nut on the cleaning module screw rod drives the cleaning module annular bracket to move up and down; the cleaning module annular bracket is fixedly connected with a cleaning module slide rail, and the cleaning module slide rail is connected with a cleaning module slide block in a sliding manner; the cleaning module annular support is provided with a cleaning module liquid detection rod and a cleaning module gas-liquid path interface, the cleaning module gas-liquid path interface is respectively a cleaning liquid interface and an external gas path interface, and the cleaning liquid interface and the external gas path interface correspond to a cleaning module external gas-liquid port sealing joint at the bottom of the cleaning module annular support; the cleaning module liquid detection rod is connected with the cleaning module liquid detection sensor.
Preferably, a cleaning module limiting optocoupler and a cleaning module limiting baffle are further arranged in the cleaning module gas-liquid path assembly and used for limiting the descending height of the cleaning module annular support; the cleaning module ring support is characterized in that a cleaning module chip sample inlet sealing joint is further arranged at the bottom of the cleaning module ring support and is located on the side face of the cleaning module external gas-liquid port sealing joint. The cleaning module controls the motor to start, the cleaning module annular support can move up and down, when the chip is cleaned, the cleaning module annular support is pressed downwards, two sealing joints (a cleaning module external gas-liquid port sealing joint and a chip sample inlet sealing joint) are respectively in sealing combination with corresponding ports on the chip, cleaning liquid is added, liquid adding is stopped when the liquid detecting rod detects liquid, external gas path gas inlet pressurization enables the cleaning liquid to flow into the waste liquid cavity, and therefore the cleaning is performed for 3-4 times in a reciprocating mode. The cleaned chip is shifted to the incubation groove by the U-shaped shifting handle, the incubation groove rises to the position corresponding to the chip detection module, the detection position is shifted by the detection shifting handle and is located on the chip carrying platform, and the carrying platform moves to enable the optical path detection component to detect the chip.
The invention is further improved in that the chip detection module comprises an optical path detection component and a detection hand-dialing component; a detection shifting handle in the detection shifting handle component shifts the chip to a detection position, the detection position is positioned on a chip carrying platform, and the chip carrying platform moves to enable the optical path detection component to detect the chip; the chip detection module is provided with an upper shielding case and a lower shielding case. Because need keep out of the sun when detecting, so chip detection module has set up two shields of upper portion shield cover and lower part shield cover, reduces the influence of ambient light to the testing result.
Preferably, the detection hand-shifting assembly is provided with a detection hand-shifting control motor, the detection hand-shifting control motor drives the detection hand-shifting control motor driving wheel to rotate, and the detection hand-shifting control motor driving wheel drives the detection hand-shifting control motor driven wheel through a detection hand-shifting control motor belt; the detection hand-shifting block is connected with a detection hand-shifting block connecting block, the detection hand-shifting block connecting block is fixedly connected with a detection hand-shifting block, and the detection hand-shifting block move along a detection hand-shifting sliding rail.
Preferably, one end of the detection dial is provided with a detection dial limiting piece which is fixedly connected with a detection dial limiting optical coupler connecting block on a bottom plate of the chip detection module and used for limiting the movement of the detection dial.
Preferably, the optical path detection assembly has a light collecting head, and an emitting plate is arranged on the upper part of the light collecting head; and a receiving plate is arranged at the lower part of the detection position, the transmitting plate transmits laser, reactants in a chip reaction cavity on the detection position are subjected to fluorescence excitation through the lighting head, then are received by the receiving plate, and a detection result is obtained by calculation after fluorescence collection and amplification.
Preferably, a first optical filter, a first lens, a second lens and a second optical filter are sequentially arranged below the emission plate from top to bottom; the receiving plate is arranged below the second optical filter; wherein the first filter and the first lens are part of the light collection head; the light collecting head is connected with a light collecting head fixing block, and the light collecting head fixing block is connected with a bottom plate of the chip detection module.
Preferably, a first shading block and a second shading block are respectively arranged on the side surfaces of the second lens and the second optical filter; the second light shielding block shields ambient light from the chip discarding channel and serves as a connecting block of the chip discarding channel.
Preferably, the chip carrier is driven by a chip carrier control motor, the chip carrier control motor drives a chip carrier screw rod to rotate, and a chip carrier motor nut connecting block on the chip carrier screw rod moves; the chip carrier motor nut connecting block is connected with the chip carrier, and the chip carrier is connected with a chip carrier sliding block I and a chip carrier sliding block II; the first chip carrier sliding block and the second chip carrier sliding block drive the chip carrier to move along the chip carrier sliding rail.
Preferably, the chip detection module is further provided with a discarding handle for dialing the detected chip to the chip discarding channel; the abandoned shifting handle is driven by a motor controlled by the abandoned shifting handle, a sliding block of the abandoned shifting handle is fixed on a top plate of the chip detection module, and a sliding rail of the abandoned shifting handle is connected with a nut connecting block of a motor of the abandoned shifting handle; the disposal shifting handle control motor drives a disposal shifting handle screw rod to rotate, the disposal shifting handle motor nut connecting block moves along the disposal shifting handle screw rod, the disposal shifting handle motor nut connecting block is connected with a vertical plate, and the vertical plate is connected with a transverse plate; one end of the abandoning shifting handle is connected with the transverse plate to serve as a abandoning shifting handle fixing part, and the other end of the abandoning shifting handle serves as a abandoning shifting handle shifting part and is used for shifting a chip to the chip abandoning channel.
Preferably, a abandoning hand shifting limiting block is fixed on the abandoning hand shifting motor nut connecting block, and a abandoning hand shifting sensing part of the abandoning hand shifting limiting block is matched with a abandoning hand shifting limiting sensor arranged on the top plate to be used for abandoning the limiting of the hand shifting.
Preferably, the two abandoning shifting handles are arranged on two sides of the transverse plate respectively.
Preferably, the time-resolved fluoroimmunoassay analyzer is further coated with a shell, the shell is provided with a display screen, and content related to detection can be viewed on the display screen; the shell is provided with a sample inlet for inserting a chip, and the side surface of the shell is also provided with a disposal groove; the time-resolved fluorescence immunoassay analyzer is further provided with a built-in printer, and a paper outlet of the built-in printer is arranged on the shell.
Compared with the prior art, the technical scheme of the invention has the beneficial effects that: the modules are arranged in the vertical direction, so that the whole structure is compact, and the occupied area is small; and the function coordination of each module is smooth and simple, and the detection efficiency is high.
Drawings
The following further detailed description of embodiments of the invention is made with reference to the accompanying drawings:
FIG. 1 is a schematic view of the internal overall structure of a time-resolved fluoroimmunoassay analyzer of the present invention;
FIG. 2 is a schematic view of the external overall structure of the time-resolved fluoroimmunoassay analyzer of the present invention;
FIG. 3 is a schematic diagram of the sample introduction module shown in FIG. 1;
FIG. 4 is a schematic diagram of a rear view of the sample injection module in FIG. 1;
FIG. 5 is a schematic diagram of a top view of a sample injection module;
FIG. 6 is a schematic diagram of a rear view of the chip loading module shown in FIG. 4;
FIG. 7 is a schematic side-bottom view of the chip loading module of FIG. 3;
FIG. 8 is a partially enlarged schematic view of the sample finger movement assembly of FIG. 3;
FIG. 9 is a schematic structural diagram of the sample injection hand lever in FIG. 6;
FIG. 10 is a partial schematic view of a chip spacing sheet structure in the sample module of FIG. 3;
FIG. 11 is a partially enlarged view of the chip spacing sheet structure of FIG. 8;
FIG. 12 is a partially enlarged top view of the chip spacing sheet structure of FIG. 8;
FIG. 13 is a schematic structural diagram of the liquid path and gas path module in FIG. 3;
FIG. 14 is a rear view of the liquid path and gas path module of FIG. 4;
FIG. 15 is a side bottom view of the liquid path and gas path module of FIG. 13;
FIG. 16 is a schematic structural view of the incubation module of FIG. 1;
FIG. 17 is an enlarged partial view of the cushioning structure of FIG. 16;
FIG. 18 is an enlarged partial view of the spacing structure of FIG. 16;
FIG. 19 is a schematic diagram of the construction of the incubation well assembly of FIG. 16;
FIG. 20 is a schematic view of the structure of the incubator in FIG. 16;
FIG. 21 is a schematic structural view of a cleaning module;
FIG. 22 is a schematic view of the cleaning module-U-shaped finger assembly of FIG. 21;
FIG. 23 is a block diagram of the purge module gas and liquid path assembly of FIG. 21;
FIG. 24 is a rear view of the purge gas and liquid assembly of FIG. 21;
FIG. 25 is a schematic diagram of a chip detection module;
FIG. 26 is a schematic side-bottom view of the chip inspection module;
FIG. 27 is a schematic view of the chip test module of FIG. 25 with the shield removed;
FIG. 28 is a cross-sectional view of the optical path components in the chip detection module;
FIG. 29 is a schematic rear view of the structure of FIG. 25 with the shield removed;
FIG. 30 is a schematic diagram of a chip carrier assembly;
FIG. 31 is a side view of the chip detection module with the shield removed;
FIG. 32 is a schematic structural view of the hands-off control assembly of FIG. 31;
FIG. 33 is a top view of the disposal paddle of FIG. 32;
wherein: 1-a base; 2-sample introduction module; 201-chip loading module; 2011-spacer plate; 2012-chip load module holder one; 2013-chip loading module bracket II; 202-liquid path gas path module; 2021-liquid circuit gas circuit control motor; 2022-chip valve control motor; 2023-ring shaped stent; 2024-liquid detection rod; 2025-liquid detection sensor; 2026-external gas-liquid port sealing joint; 2027-chip valve lever; 2028-external gas-liquid interface; 2029-liquid path gas path slide rail; 20210-liquid circuit gas circuit slider; 20211-liquid path gas circuit lead screw; 20212-liquid circuit gas circuit motor nut; 20213-side stand; 20214-chip valve slide; 20215-chip valve slide; 20216-chip valve screw; 20217-chip valve motor nut; 20218-chip valve slider connection block; 20219-plate; 20220-chip valve limit optical coupler; 20221-chip valve limit stop piece; 20222-liquid path gas path limit stop piece; 20223-liquid path gas path limit optical coupler; 20224-sample inlet interface; 20225-sample inlet sealing head; 203-sample introduction and poking of the chip; 2031-sample injection chip hand-shifting slide bar; 2032-sample injection handle chute; 2033-chip insertion sensor; 204-chip sample injection groove; 2041-sample introduction tank movement motor; 2042-sample groove motor nut; 2043-sample introduction groove screw rod; 2044-sample introduction groove connecting block I; 2045-sample introduction groove connecting block II; 20451-a first sample feeding groove connecting block II; 20452-second sample feeding groove connecting block II; 2046-sample introduction groove slide block; 20461-sample introduction groove slide block I; 20462-sample introduction groove sliding block II; 2047-sample introduction groove slide rail; 20471-sample introduction groove slide rail I; 20472-sample introduction groove slide rail II; 205-chip sample introduction hand-dialing motion assembly; 2051-horizontal movement of the sample injection hand; 2052-sample feeding hand shifting horizontal slide rail; 2053-sample feeding hand shifting horizontal movement sliding block connecting block; 2054-slide rail for up-and-down movement of sample feeding handle of chip; 2055-sample feeding handle up-and-down moving slide block; 2056-sample feeding hand-dialing horizontal motor; 2057-sample introduction handle-shifting belt connecting block; 2058-sample introduction hand-dialing horizontal belt; 2059-sample introduction hand-poking motion driving wheel; 20510-sample feeding hand moving driven wheel; 206-chip spacing piece structure; 2061-chip spacing sheet; 2062-limiting slide bar; 2063-chip spacing sheet chute; 2064-chip spacing sheet slide rail; 2065-chip spacing piece slide block; 207-chip code scanner; 208-a top plate; 2081-a through hole; 3-an incubation module; 301-an incubation well assembly; 30101-a heating plate; 30102-heat-insulating sheet; 30103-an incubation well; 302-incubator slide rail; 303-connecting block of incubation groove; 304-a band-type brake motor; 305-moving a belt in the incubation groove; 306-the incubation groove moving driving wheel; 307-the incubation groove moves the driven wheel; 308-incubator slide rack; 3081-cushioning pad; 309-buffer block; 3010-limiting stop piece; 3011-limiting optocoupler connecting blocks; 3012-limit optocoupler; 3013-pressing the chip; 3014-a nut; 4-a cleaning module; 401-washing the side stand; 402-cleaning module gas-liquid path components; 4021-the cleaning module controls the motor; 4022-cleaning module screw rod; 4023-cleaning module motor nut; 4024-cleaning module ring support; 4025-cleaning module slide rails; 4026-cleaning module slide block; 4027-cleaning module liquid detection rod; 4028-cleaning module gas-liquid path interface; 40281-cleaning solution interface; 40282-external gas circuit interface; 4029-cleaning module liquid detection sensor; 40210-cleaning module limit optical coupler; 40211-cleaning module limit baffle sheet; 40212-cleaning module chip sample inlet sealing joint; 40213-the cleaning module is externally connected with a gas-liquid port sealing joint; 403-U-shaped finger; 4031-U-shaped first hand shifting arm; 4032-U-shaped second hand-shifting arm; 404-U-shaped hand-pulling control motor; 405-a U-shaped thumbwheel; 406-a U-shaped thumb strap; 407-U-shaped hand-pulling driven wheel; 408-U-shaped shifting handle belt connecting block; 409-U-shaped shifting block components; 4010-U-shaped thumbpiece slide rail; 5-chip detection module; 501-optical path detection components; 5011-a light-harvesting head; 50111-lighting head fixing block; 5012-a transmitter plate; 5013-a receiving plate; 5014-first filter; 5015-first lens; 5016-second lens; 5017-a second filter; 5018 — first light blocking block; 5019-second light blocking block; 502-detect the paddle assembly; 5021, detecting a hand-pulling control motor; 5022, detecting the hand-pulling to control the motor driving wheel; 5023, detecting a hand-pulling control motor belt; 5024, detecting a hand-pulling control motor driven wheel; 5025, detecting a connecting block of a shifting handle sliding block; 5026, detecting a hand shifting slider; 5027 detecting the sliding rail of the hand; 503-detecting the dial; 5031-detecting a finger-poking limiting sheet; 5032-detecting the hand-pulling limit optical coupling connecting block; 5033-detecting the hand-pulling limit optical coupler; 504-chip carrier; 5041-chip carrier control motor; 5042-chip carrier screw; 5043-nut connecting block of motor of chip carrier; 5044-chip carrier slide block one; 5045-chip carrier slide block two; 5046-slide rail of chip carrier; 505-an upper shield; 506-lower shield can; 507-a bottom plate; 508-chip discard lanes; 509-abandon the hand; 5091 discarding the hand control motor; 5092-discarding the hand-shifting slider; 5093 discarding the handle-shifting slide rail; 5094 discarding a nut connecting block of a hand-pulling motor; 5095-discarding the thumb screw; 5096-riser; 5097-horizontal plate; 5098-discarding the handle-pulling limiting block; 5099-discarding the toggle part of the hand; 50100-discarding the hand-dialing induction part; 50910-discarding the handle fixing part; 5010-top plate; 50101-abandoning a hand-pulling limit sensor; 6-a shell; 7-a display screen; 8-a sample inlet; 9-abandoning the tank; 10-a built-in printer; 1001-paper exit.
Detailed Description
As shown in fig. 1, the time-resolved fluoroimmunoassay analyzer of the present invention is a schematic diagram of an internal overall structure thereof, and comprises a base 1, a sample introduction module 2, an incubation module 3, a cleaning module 4, and a chip detection module 5;
the sample injection module 2 is provided with a chip loading module 201 and a liquid path and gas path module 202, the chip loading module 201 is used for carrying a chip to the liquid path and gas path module 202, and the liquid path and gas path module 202 is used for enabling a sample in the chip to flow to a preset position; thereafter the chip is transported into the incubation module 3;
the incubation module 3 enables the chip to react at a constant temperature within a set temperature;
the incubation module 3 freely moves up and down along the vertical direction, and the sample introduction module 2, the cleaning module 4 and the chip detection module 5 are respectively arranged on the side surface of the incubation module 3 in the vertical movement direction; the chip cleaned in the cleaning module 4 is sent to the chip detection module 5 for detection by the incubation module 3;
as shown in fig. 2, the time-resolved fluoroimmunoassay analyzer is further covered with a casing 6, a display screen 7 is arranged on the casing 6, and the content related to detection can be viewed on the display screen 7; the front surface of the shell 6 is provided with a sample inlet 8 for inserting a chip, and the side surface of the shell 6 is also provided with a disposal groove 9; the time-resolved fluoroimmunoassay analyzer is further provided with a built-in printer 10, and a paper outlet 1001 of the built-in printer 10 is provided on the housing 6.
As shown in fig. 3 to 12, the chip loading module 201 includes a chip sampling dial 203, and the chip sampling dial 203 dials a chip to be put in place in a chip sampling slot 204; a chip limiting piece structure 206 is arranged behind the chip sample feeding groove 204; the chip is driven by the chip sample feeding groove 204 to move;
as shown in fig. 8, the chip sample feeding hand lever 203 is controlled by a chip sample feeding hand lever motion assembly 205; the chip sample injection hand shifting motion assembly comprises a sample injection hand shifting horizontal motion assembly and a sample injection hand shifting up-and-down motion assembly; the sample introduction hand dialing horizontal movement component is provided with a sample introduction hand dialing horizontal movement sliding block 2051, the sample introduction hand dialing horizontal movement sliding block 2051 moves along a sample introduction hand dialing horizontal sliding rail 2052, the sample introduction hand dialing horizontal movement sliding block 2051 is connected with a sample introduction hand dialing horizontal movement sliding block connecting block 2053, a chip sample introduction hand dialing up-and-down movement sliding rail 2054 is fixedly connected onto the sample introduction hand dialing horizontal movement sliding block connecting block 2053, a sample introduction hand dialing up-and-down movement sliding block 2055 is connected onto the chip sample introduction hand dialing up-and-down movement sliding rail 2054, the sample introduction hand dialing up-and-down movement sliding block 2055 is connected with the chip sample introduction hand dialing 203, as shown in fig. 9, a chip sample introduction hand dialing sliding rod 2031 is arranged on the chip sample introduction hand dialing 203, and the chip sample introduction hand dialing sliding rod 2031 moves; that is, the chip sample feeding hand lever 203 can move horizontally and vertically along the sample feeding hand lever chute 2032;
as shown in fig. 3-4, the chip sample feeding groove 204 is controlled by the sample feeding groove moving motor 2041, the sample feeding groove motor nut 2042 moves along the sample feeding groove lead screw 2043, the sample feeding groove motor nut 2042 is connected with the sample feeding groove connecting block 2044, the sample feeding groove connecting block 2044 is connected with the chip sample feeding groove 204, the sample feeding groove connecting block 2045 is connected with the sample feeding groove connecting block 2044, the sample feeding groove connecting block 2045 is connected with the sample feeding groove sliding block 2046, and the sample feeding groove sliding block 2046 reciprocates along the sample feeding groove sliding rail 2047.
As shown in fig. 5 to 6, the partition 2011 of the chip loading module 201 has two sample feeding slot slide rails 2047, which are a first sample feeding slot slide rail 20471 and a second sample feeding slot slide rail 20472 respectively; correspondingly, two sample feeding groove sliding blocks 2046 are respectively a first sample feeding groove sliding block 20461 and a second sample feeding groove sliding block 20462, and two sample feeding groove connecting blocks 2045 are respectively a first sample feeding groove connecting block two 20451 and a second sample feeding groove connecting block two 20452; the first sample feeding groove connecting block two 20451 is connected with the sample feeding groove sliding block one 20452, the sample feeding groove sliding block one 20461 reciprocates along the sample feeding groove sliding rail one 20471, the second sample feeding groove connecting block two 20452 is connected with the sample feeding groove sliding block two 20462, and the sample feeding groove sliding block two 20462 reciprocates along the sample feeding groove sliding rail two 20472;
as shown in fig. 10 to 12, the partition 2011 has a first chip loading module support 2012 and a second chip loading module support 2013, wherein the second chip loading module support 2013 is provided with a chip limiting piece chute 2063; the chip spacing piece structure 206 is provided with a chip spacing piece 2061, and a chip spacing piece sliding rod 2062 for moving in the chip spacing sliding groove 2063 is arranged on the chip spacing piece 2061; spacing piece slide rail 2064 of fixedly connected with chip on the lateral wall of chip advance kind groove 204, be connected with spacing piece slider 2065 of chip on the spacing piece slide rail 2064 of chip, spacing piece 2061 of chip is connected on the spacing piece slider 2065 of chip, spacing piece 2061 of chip follows chip advance kind groove 204 horizontal movement, the cooperation simultaneously spacing piece spout 2063 up-and-down motion of chip. The sliding groove of the first 2012 chip loading module support in the technical scheme has no specific function, and is convenient to process and manufacture in order to be uniform with the structure of the second 2013 chip loading module support.
As shown in fig. 6, the front end of the sample pushing handle chute 2032 is higher than the rear end, so that the sample pushing handle chute 2032 has a high starting point; the sample introduction hand-shifting horizontal movement component is provided with a sample introduction hand-shifting horizontal motor 2056, one end of a sample introduction hand-shifting belt connecting block 2057 is fixedly connected to the sample introduction hand-shifting horizontal belt 2058, and the other end of the sample introduction hand-shifting belt connecting block 2057 is fixedly connected with the sample introduction hand-shifting horizontal movement sliding block connecting block 2053; the sample introduction hand shifting horizontal belt 2058 is sleeved on the sample introduction hand shifting motion driving wheel 2059 and the sample introduction hand shifting motion driven wheel 20510, the sample introduction hand shifting driving wheel 2059 is connected with the sample introduction hand shifting horizontal motor 2056, and the sample introduction hand shifting horizontal motion sliding block connecting block 2053 is sleeved on the sample introduction hand shifting horizontal belt 2058; advance kind and dial horizontal motor 2056 and start, the chip advances kind and dials the hand 203 and moves along advancing kind and dial horizontal slide rail 2052 of hand, the initial position that the chip advances kind and dials the hand 203 is in chip introduction groove 204 front end top (advance kind and dial the high starting point of hand chute), the chip is put into the back, the chip inserts behind the sensing of inductor 2033 to the chip, the chip advances kind and dials the hand 203 and moves along advancing kind and dial the hand spout 2032, it sets up to be higher than the rear end to advance kind and dial the front end of hand spout 2032, the purpose is that not block the chip and put into chip introduction groove 204, the chip advances kind and dials the hand 203 and dials the chip and makes the chip arrive chip and advance kind groove 204 and predetermine the.
As shown in fig. 13 to 15, the liquid path and gas path module 202 includes a liquid path and gas path control motor 2021 and a chip valve control motor 2022; the liquid path and gas path control motor 2021 drives the annular bracket 2023 to move downwards, the annular bracket 2023 is pressed downwards to enable the sample inlet port 20224 on the annular bracket 2023 to be in sealed connection with the sample inlet cavity of the chip through the sample inlet port sealing head 20225, and the external gas-liquid port 2028 is in sealed connection with the external gas-liquid port of the chip through the external gas-liquid port sealing head 2026; a liquid detection rod 2024 is fixedly connected to the annular bracket 2023, and the liquid detection rod 2024 is connected with a liquid detection sensor 2025; the chip valve control motor 2022 drives the chip valve slider connecting block 20218 to move, the chip valve slider connecting block 20218 is provided with a chip valve pressing rod 2027, and the chip valve pressing rod 2027 presses down the valve of the chip under the control of the chip valve control motor 2022.
As shown in fig. 14, the liquid path and gas path module 202 further includes a liquid path and gas path slide rail 2029 and a liquid path and gas path slide block 20210; the liquid path and gas path control motor 2021 is started, the liquid path and gas path lead screw 20211 rotates, the liquid path and gas path motor nut 20212 on the liquid path and gas path lead screw 20211 drives the annular bracket 2023 to move downwards, the annular bracket 2023 is connected with the liquid path and gas path slide rail 2029 and is matched with the liquid path and gas path slide block 20210 to move, and the liquid path and gas path slide block 20210 is fixedly connected to the inner side of the side bracket 20213 of the liquid path and gas path module 202.
As shown in fig. 15, the liquid path and gas path module 202 further includes a chip valve slide rail 20214 and a chip valve slider 20215; the chip valve control motor 2022 is started, the chip valve lead screw 20216 rotates, the chip valve motor nut 20217 on the chip valve lead screw 20216 drives the chip valve slider connection block 20218 to move, the chip valve slider connection block 20218 is connected with the chip valve slider 20215, and the chip valve slide rail 20214 is installed inside the support plate 20219 of the liquid path and gas path module 202. The liquid path and gas path control motor 2021 is started, the liquid path and gas path lead screw 20211 rotates, the liquid path and gas path motor nut 20212 drives the annular bracket 2023 to move downwards, the annular bracket 2023 is connected with the liquid path and gas path slide rail 2029 and moves in coordination with the liquid path and gas path slide block 20210, the liquid path and gas path slide block 20210 is fixedly connected with the inner side of the side bracket 20213, the annular bracket 2023 is pressed downwards to enable a sample inlet port interface 20224 (air inlet and pressure action) on the annular bracket 2023 to seal a sample injection cavity of the butt joint chip through a sample inlet port sealing head 20225, an external gas and liquid port interface 2028 (sealing treatment, gas and liquid can not be introduced) is sealed and connected with an external gas and liquid port of the butt joint chip through an external gas and liquid port sealing joint 2026, then the sample in the sample injection cavity of the chip is slowly advanced along a micro flow path of the chip by the air inlet port interface 20224 pressure, a liquid detection rod 2024 is fixedly, when a sample in the chip reaches the chip conductive rubber valve, the liquid detection rod senses capacitance change, a chip valve control motor 2022 of the instrument is started, a chip valve lead screw 20216 rotates, a chip valve motor nut 20217 drives a chip valve slider connecting block 20218 to move, the chip valve slider connecting block 20218 is connected with a chip valve slider 20215, a chip valve slide rail 20214 is installed on the inner side of the supporting plate 20219, a chip valve pressing rod 2027 is installed on the chip valve slider connecting block 20218, the chip valve pressing rod 2027 presses down and presses the valve under the control of the chip valve control motor 2022, so that chip liquid cannot continue to advance, and meanwhile, the sample inlet port 20224 stops air intake and pressurization.
As shown in fig. 13, the liquid path and gas path module 202 is further provided with a chip valve limit optical coupler 20220 and a chip valve limit baffle 20221, which are used for limiting the descending height of the chip valve pressure lever 2027; the liquid path and gas path module 202 is further provided with a liquid path and gas path limit optical coupler 20223 and a liquid path and gas path limit baffle 20222 for limiting the descending height of the annular bracket 2023.
The liquid path and gas path module 202 further includes a chip code scanner 207 for scanning the two-dimensional code on the chip. The chip code scanner 207 is positioned above the chip, and the code is scanned through the through hole 2081 on the top plate 208 of the sample injection module 2, so that whether the chip detection items are correct or not is confirmed, whether the chip is in the valid period or not is judged, and if the chip does not meet the requirement, a machine alarms to prompt error information.
As shown in FIGS. 16-20, the incubation module 3 has an incubation well assembly 301. as shown in FIG. 17, the incubation well assembly 301 includes a heat patch 30101 and a heat preservation patch 30102; as shown in FIG. 19, a plurality of stacked incubators 3013 in the incubator assembly 301, and the plurality of stacked incubators 30103 move freely up and down along the incubator slide rails 302. The incubator assembly 301 is provided with a heater 30101 and a heat-retaining plate 30102 to allow isothermal reaction of the chips at a predetermined temperature.
As shown in fig. 16, the incubation well assembly 301 is further provided with an incubation well connection block 303; the incubation groove connecting block 303 is connected with an incubation groove moving belt 305, and a band-type brake motor 304 drives an incubation groove moving driving wheel 306, and the incubation groove moving driving wheel 307 is driven by the incubation groove moving belt 305 to rotate, so that a plurality of stacked incubation grooves 3013 in the incubation groove assembly 301 move up and down freely along the incubation groove sliding rail 302.
The incubation groove slide rail 302 is arranged on the incubation groove slide rail bracket 308, the side surface of the incubation groove assembly 301 is provided with a buffer block 309, and correspondingly, the bottom of the incubation groove slide rail bracket 308 is provided with a buffer pad 3081; the temperature incubation groove component 301 is provided with a limiting blocking piece 3010, the temperature incubation groove sliding rail support 308 is fixedly connected with a limiting optical coupler connecting block 3011, and a limiting optical coupler 3012 is installed on the limiting optical coupler connecting block 3011 and used for limiting the temperature incubation groove 30103 to move up and down.
As shown in FIG. 20, the incubation slot 30103 is provided with a chip strip 3012, and two protrusions of the chip strip 3012 are engaged with grooves in the chip and fixed by nuts 3014 for positioning the incubation reaction on the chip.
The heating sheet 30101 and the heat-insulating sheet 30102 are disposed on the side of the plurality of stacked incubators 30103.
As shown in fig. 21-24, the cleaning module 4 comprises a U-shaped handle assembly and a cleaning module gas-liquid path assembly 402; after the incubation reaction of the chip is finished, a U-shaped hand driver 403 in the U-shaped hand driver component drives the chip to a cleaning position in the cleaning module gas-liquid path component 402 for cleaning; after the chip cleaning is finished, the U-shaped poking hand 403 dials back the chip again; the washing module 4 further comprises washing side brackets 401 on both sides.
As shown in fig. 22, the U-shaped shifting block assembly is provided with a U-shaped shifting block control motor 404, under the control of the U-shaped shifting block control motor 404, a U-shaped shifting driving wheel 405 rotates to drive a U-shaped shifting driven wheel 407 through a U-shaped shifting belt 406, a U-shaped shifting belt connecting block 408 on the U-shaped shifting belt 406 is connected with a U-shaped shifting block assembly 409, and the U-shaped shifting block assembly 409 is connected with the U-shaped shifting block 403 to move along a U-shaped shifting slide rail 4010.
The U-shaped hand driver 403 extends towards two sides to form a first U-shaped hand driver 4031 and a second U-shaped hand driver 4032 respectively, and the distance between the first U-shaped hand driver 4031 and the second U-shaped hand driver 4032 is greater than the length of a chip.
The cleaning module gas-liquid circuit assembly 402 is provided with a cleaning module control motor 4021, the cleaning module control motor 4021 is started, a cleaning module screw 4022 rotates, and a cleaning module motor nut 4023 on the cleaning module screw 4022 drives the cleaning module annular support 4024 to move up and down; the cleaning module annular support 4024 is fixedly connected with a cleaning module slide rail 4025, and the cleaning module slide rail 4025 is slidably connected with a cleaning module slide block 4026; a cleaning module liquid detection rod 4027 and a cleaning module gas-liquid path interface 4028 are arranged on the cleaning module annular support 4024, the cleaning module gas-liquid path interface 4028 is a cleaning liquid interface 40281 and an external gas path interface 40282 respectively, and the cleaning liquid interface 40281 and the external gas path interface 40282 correspond to a cleaning module external gas-liquid path sealing joint 40213 at the bottom of the cleaning module annular support 4024; the cleaning module liquid detection rod 4027 is connected to a cleaning module liquid detection sensor 4029.
A cleaning module limiting optocoupler 40210 and a cleaning module limiting baffle 40211 are further arranged in the cleaning module gas-liquid circuit assembly 402 and used for limiting the descending height of the cleaning module annular support 4024; cleaning module ring support 4024 bottom still is provided with cleaning module chip introduction port sealing joint 40212, cleaning module chip introduction port sealing joint 40212 is located cleaning module external gas-liquid mouth sealing joint 40213's side. Cleaning module control motor 4021 starts, cleaning module ring support 4024 can up-and-down motion, when carrying out the chip washing, cleaning module ring support 4024 pushes down, two sealing joint (cleaning module external gas-liquid mouth sealing joint 40213 and chip introduction port sealing joint 40212) respectively with on the chip correspond a mouthful sealing combination, the washing liquid is added, the liquid feeding stops when cleaning module liquid feeler lever 4027 detects liquid, external gas circuit air admission pressurization makes the washing liquid flow in waste liquid chamber, so reciprocal washing 3-4 times. The cleaned chip is shifted into the incubation groove 30103 by the U-shaped shifting handle 403, the incubation groove 30103 is lifted to the position corresponding to the chip detection module 5, and is shifted to the detection position by the detection shifting handle 503, and the detection position is located on the chip carrier 504, so that the optical path detection assembly 501 detects the chip by moving the chip carrier 504.
As shown in fig. 25 to 31, the chip detection module 5 includes an optical path detection component 501 and a detection handle component 502; a detection dial 503 in the detection dial assembly 502 dials the chip to a detection position, the detection position is located on a chip carrier 504, and the chip carrier 504 moves to enable the optical path detection assembly 501 to detect the chip; the chip detection module 5 is provided with an upper shield 505 and a lower shield 506. Because light shielding is needed during detection, the chip detection module 5 is provided with two shielding cases, namely an upper shielding case 505 and a lower shielding case 506, so that the influence of ambient light on the detection result is reduced.
The detection hand pusher assembly 502 is provided with a detection hand pusher control motor 5021, the detection hand pusher control motor 5021 drives the detection hand pusher control motor driving wheel 5022 to rotate, and the detection hand pusher control motor driving wheel 5022 drives the detection hand pusher control motor driven wheel 5024 through a detection hand pusher control motor belt 5023; the detection hand dial 503 is connected with a detection hand dial slider connecting block 5025, the detection hand dial slider connecting block 5025 is fixedly connected with a detection hand dial slider 5026, and the detection hand dial slider 5026 and the detection hand dial 503 move along a detection hand dial sliding rail 5027.
One end of the detection hand-dialing member 503 is provided with a detection hand-dialing limiting piece 5031, and a detection hand-dialing limiting optical coupler connecting block 5032 arranged on the bottom plate 507 of the chip detection module 5 is fixedly connected with a detection hand-dialing limiting optical coupler 5033 for limiting the movement of the detection hand-dialing member 503.
As shown in fig. 29, the optical path detecting assembly 501 has a light collecting head 5011, and a transmitting plate 5012 is disposed above the light collecting head 5011; the receiving plate 5013 is arranged below the detection position, the transmitting plate 5012 transmits laser, reactants in a chip reaction cavity on the detection position are subjected to fluorescence excitation through the light collecting head 5011, and then are received by the receiving plate 5013, and a detection result is obtained through fluorescence collection and amplification and calculation.
As shown in fig. 28, below the emission plate 5012, a first filter 5014, a first lens 5015, a second lens 5016, and a second filter 5017 are provided in this order from top to bottom; the receiving plate 5013 is disposed below the second filter 5017; wherein the first filter 5014 and the first lens 5015 are part of the lighting head 5011; the lighting head 5011 is connected with a lighting head fixing block 50111, and the lighting head fixing block 50111 is connected with a bottom plate 507 of the chip detection module 5.
The side surfaces of the second lens 5015 and the second optical filter 5017 are respectively provided with a first light-shielding block 5018 and a second light-shielding block 5019; the second light shielding block 5019 shields ambient light from the chip disposal channel 508 while serving as a connection block for the chip disposal channel 508.
As shown in fig. 30, stage 504 is driven by stage control motor 5041, stage control motor 5041 drives stage screw 5042 to rotate, and stage motor nut connection block 5043 on stage screw 5042 moves; the chip carrier motor nut connecting block 5043 is connected with the chip carrier 504, and the chip carrier 504 is connected with a first chip carrier sliding block 5044 and a second chip carrier sliding block 5045; the first stage slider 5044 and the second stage slider 5045 drive the stage 504 to move along a stage slide 5046.
As shown in fig. 32 to 33, the chip detection module 5 is further provided with a discarding pusher 509 for pushing the detected chip into the chip discarding channel 508; the disposal hand shifting 509 is driven by a disposal hand shifting control motor 5091, a disposal hand shifting slider 5092 is fixed on a top plate 5010 of the chip detection module 5, and a disposal hand shifting slide 5093 is connected with a disposal hand shifting motor nut connecting block 5094; the abandoning shifting handle control motor 5091 drives the abandoning shifting handle screw 5095 to rotate, the abandoning shifting handle motor nut connecting block 5094 moves along the abandoning shifting handle screw 5095, the abandoning shifting handle motor nut connecting block 5094 is connected with a vertical plate 5096, and the vertical plate 5096 is connected with a horizontal plate 5097; one end of the disposing handle 509 is connected to the horizontal plate 5097 as a disposing handle fixing portion 50910, and the other end of the disposing handle 509 as a disposing handle striking portion 5099 is used for striking a chip into the chip disposing passage 508.
A disposal handle stopper 5098 is fixed on the disposal handle motor nut connecting block 5094, and a disposal handle sensing part 50100 of the disposal handle stopper 5098 is matched with a disposal handle limit sensor 50101 mounted on the top plate 5010 to limit a disposal handle 509; the two disposal poking hands 509 are arranged on two sides of the transverse plate 5097 respectively.
When in specific use:
the sample injection module 2 is specifically divided into a chip loading module 201 and a liquid path and gas path module 202; firstly, a chip is put in from an instrument sample inlet 8, a chip code scanner 207 on the liquid path and gas path module 202 scans a two-dimensional code of the chip to confirm whether a chip detection item is correct or not and whether the chip is in a valid period or not, and if the chip is not in line with the valid period, a machine alarms to prompt error information. Meanwhile, the chip insertion sensor 2033 senses the chip, the chip sampling hand-lever 203 slides, the chip is shifted to be put in place in the chip sampling groove 204, and the chip limiting piece structure 206 is arranged behind the chip sampling groove 204 to prevent the chip from being put in a preset position exceeding the chip sampling groove 204. Then the chip is driven by the chip sample injection groove 204 to move to the liquid path and gas path module 202, the liquid path and gas path control motor 2021 is started, the annular bracket 2023 is pressed down to enable the sample injection port interface 20224 (air inlet pressurization effect) on the annular bracket 2023 to seal the sample injection cavity of the butt chip, the external gas and liquid port interface 2028 (sealing treatment, gas and liquid can not be fed) seals the external gas and liquid port of the butt chip, then the sample in the chip sample injection cavity is slowly advanced along the chip micro-channel by air inlet pressurization through the sample injection port connector, when the chip conductive rubber valve is reached, the liquid detection rod 2024 senses capacitance change, the chip valve control motor 2022 of the instrument is started, and the chip valve pressing rod 2027 presses down the valve under the control of the motor to enable the chip liquid not to continue to advance.
The chip sample feeding hand 203 stirs the chip under the control of the motor to make the chip reach the incubation module 3, and the incubation module 3 is provided with a heating sheet 30101 and a heat preservation sheet 30102 to make the chip reaction cavity perform sample immunoreaction at a preset temperature.
After the reaction, the U-shaped hand 403 of washing module 4 stirs the chip to wash module 4, it can wash the chip after the reaction to wash module 4 to have washing module gas-liquid way subassembly 402, wash whether the washing module liquid feeler lever 4027 on the module ring support 4024 can the sensing liquid reaches valve department, the sensing gives the signal after promptly to the external liquid way gas crossing of chip pressurize of admitting air, make the liquid after the washing get into the waste liquid chamber of chip, so relapse 3-4 times after, the chip is washd and is accomplished, U-shaped hand 403 stirs the chip immediately and stirs chip to incubation module 3, incubation module 3 rises to chip detection module 5 corresponding position.
The detection hand-piece 503 of the chip detection module 5 pulls the chip to reach the chip carrier 504, the chip carrier 504 can move, so that the optical signal detection is performed on the chip reaction chamber by the optical path detection component 501, and after the detection is completed, the disposal hand-piece 509 pulls the chip to enter the disposal groove 9 under the control of the motor for disposal of the biological waste. The detection result can be displayed on the display screen 7 of the instrument, and the detection result can be printed by the built-in printer 10.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications, such as changes in the position of various elements mounted on a base, may be made without departing from the spirit and scope of the invention, and these changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (33)
1. A time-resolved fluoroimmunoassay analyzer comprises a base, and is characterized by also comprising a sample introduction module, an incubation module, a cleaning module and a chip detection module;
the sample injection module is internally provided with a chip loading module and a liquid path and gas path module, the chip loading module is used for carrying a chip to the liquid path and gas path module, and the liquid path and gas path module is used for enabling a sample in the chip to flow to a preset position; thereafter the chip is transported into the incubation module;
the incubation module enables the chip to react at constant temperature within a set temperature;
the incubation module freely moves up and down along the vertical direction, and the sample introduction module, the cleaning module and the chip detection module are respectively arranged on the side surface of the incubation module in the vertical movement direction; and conveying the chip cleaned in the cleaning module to the chip detection module for detection by the incubation module.
2. The time-resolved fluoroimmunoassay analyzer of claim 1, wherein the incubation module has an incubation well assembly comprising a heat patch and a heat-retention patch; a plurality of stacked incubators in the incubator assembly, the plurality of stacked incubators being free to move up and down along the incubator slide.
3. The time-resolved fluoroimmunoassay analyzer of claim 2, wherein the incubation well assembly is further provided with an incubation well connection block; the incubation groove connecting block is connected with an incubation groove moving belt, the band-type brake motor drives the incubation groove moving driving wheel, the incubation groove moving belt drives the incubation groove moving driven wheel to rotate, and therefore the multiple stacked incubation grooves in the incubation groove assembly can move up and down freely along the incubation groove sliding rail.
4. The time-resolved fluoroimmunoassay analyzer of claim 3, wherein the incubator slide rail is disposed on an incubator slide rail bracket, a buffer block is disposed on a side of the incubator assembly, and a buffer pad is disposed on a bottom of the incubator slide rail bracket; the incubator is characterized in that a limiting separation blade is arranged on the incubator assembly, a limiting optical coupler connecting block is fixedly connected to the incubator slide rail support, and a limiting optical coupler is mounted on the limiting optical coupler connecting block and used for limiting the up-and-down movement of the incubator.
5. The time-resolved fluoroimmunoassay analyzer of claim 4, wherein the incubation well is provided with a chip bead, and two protrusions of the chip bead are engaged with the grooves of the chip for positioning the chip for incubation reaction.
6. The time-resolved fluoroimmunoassay analyzer of claim 5, wherein the heat-retaining plate and the heat-retaining plate are disposed on the side of the plurality of stacked incubators.
7. The time-resolved fluoroimmunoassay analyzer of claim 1, wherein the chip loading module comprises a chip sample feeding paddle, and the chip sample feeding paddle shifts the chip to be put in place in the chip sample feeding slot; a chip limiting piece structure is arranged behind the chip sample feeding groove; the chip is driven by the chip sample feeding groove to move.
8. The time-resolved fluoroimmunoassay analyzer of claim 7, wherein the chip sample hand is controlled by a chip sample hand movement assembly; the chip sample injection hand shifting motion assembly comprises a sample injection hand shifting horizontal motion assembly and a sample injection hand shifting up-and-down motion assembly; advance kind and dial hand horizontal movement subassembly and have advance kind and dial hand horizontal movement slider, advance kind and dial hand horizontal movement slider along advance kind and dial hand horizontal sliding rail motion, it advances kind and dials hand horizontal movement slider connecting block to connect on the hand horizontal movement slider to advance kind, advance fixedly connected with chip advance kind and dial hand up-and-down motion slide rail on the hand horizontal movement slider connecting block, it advances kind and dials hand up-and-down motion slider to be connected with on the hand up-and-down motion slide rail to advance kind on the chip advance kind, advance kind and dial hand up-and-down motion slider with the chip advances kind and dial the hand and connect, it advances kind and dial the hand slide bar to be provided with the chip on the hand to advance kind to the chip, the chip advances.
9. The time-resolved fluoroimmunoassay analyzer of claim 8, wherein the chip well is controlled by a well motor, a well motor nut moves along a well lead screw, the well motor nut is connected with a well connecting block I, the well connecting block I is connected with the chip well, a well connecting block II is connected with the well connecting block I, the well connecting block II is connected with a well slider, and the well slider reciprocates along a well slide rail.
10. The time-resolved fluoroimmunoassay analyzer of claim 9, wherein the partition plate of the chip loading module has two sample injection slot slide rails, respectively a first sample injection slot slide rail and a second sample injection slot slide rail; correspondingly, the number of the sample feeding groove sliding blocks is two, namely a first sample feeding groove sliding block and a second sample feeding groove sliding block, and the number of the second sample feeding groove connecting blocks is two, namely a first sample feeding groove connecting block and a second sample feeding groove connecting block; the first sample injection groove connecting block II is connected with the first sample injection groove sliding block I, the first sample injection groove sliding block I moves in a reciprocating mode along the first sample injection groove sliding rail, the second sample injection groove connecting block II is connected with the second sample injection groove sliding block II, and the second sample injection groove sliding block II moves in a reciprocating mode along the second sample injection groove sliding rail.
11. The time-resolved fluoroimmunoassay analyzer of claim 9, wherein the partition plate has a first chip loading module support and a second chip loading module support, wherein the second chip loading module support is provided with a chip limiting plate sliding groove; the chip limiting piece structure is provided with a chip limiting piece, and a chip limiting piece sliding rod used for moving in the chip limiting sliding groove is arranged on the chip limiting piece; the chip advances a spacing slide rail of fixedly connected with chip on the lateral wall of appearance groove, be connected with the spacing piece slider of chip on the spacing piece slide rail of chip, the spacing piece of chip is connected on the spacing piece slider of chip, the spacing piece of chip is followed the appearance groove horizontal motion of chip, cooperates simultaneously spacing piece spout up-and-down motion of chip.
12. The time-resolved fluoroimmunoassay analyzer of claim 8, wherein the front end of the sample hand-pushing chute is higher than the rear end, so that the sample hand-pushing chute has a high starting point; advance kind and dial hand horizontal movement subassembly has advance kind and dial hand horizontal motor, advances kind and dial hand belt connecting block one end fixed connection advance kind on the horizontal belt of hand, advance kind dial hand belt connecting block the other end with advance kind and dial hand horizontal movement slider connecting block fixed connection.
13. The time-resolved fluoroimmunoassay analyzer of claim 1, wherein the liquid path and gas path module comprises a liquid path and gas path control motor and a chip valve control motor; the liquid path gas circuit control motor drives the annular support to move downwards, the annular support is pressed downwards to enable a sample inlet interface on the annular support to seal a sample inlet cavity of the butt chip through a sample inlet sealing head, and an external gas-liquid port interface seals an external gas-liquid port of the butt chip through an external gas-liquid port sealing head; a liquid detection rod is fixedly connected to the annular support and is connected with a liquid detection sensor; the chip valve control motor drives the chip valve sliding block connecting block to move, a chip valve pressing rod is mounted on the chip valve sliding block connecting block, and the chip valve pressing rod presses down a valve pressing the chip under the control of the chip valve control motor.
14. The time-resolved fluoroimmunoassay analyzer of claim 13, wherein the liquid path and gas path module further comprises a liquid path and gas path slide rail and a liquid path and gas path slide block; the liquid path and gas path control motor is started, the liquid path and gas path lead screw rotates, a liquid path and gas path motor nut on the liquid path and gas path lead screw drives the annular support to move downwards, the annular support is connected with the liquid path and gas path slide rail and moves in a matched mode with the liquid path and gas path slide block, and the liquid path and gas path slide block is fixedly connected to the inner side of the side support of the liquid path and gas path module.
15. The time-resolved fluoroimmunoassay analyzer of claim 14, wherein the liquid path and gas path module further comprises a chip valve slide rail and a chip valve slider; the chip valve control motor starts, and the chip valve lead screw is rotatory, chip valve motor nut on the chip valve lead screw drives chip valve slider connecting block motion, chip valve slider connecting block with chip valve slider connects, chip valve slide rail is installed the extension board inboard of liquid way gas circuit module.
16. The time-resolved fluoroimmunoassay analyzer of claim 15, wherein the liquid path and gas path module is further provided with a chip valve limiting optocoupler and a chip valve limiting baffle for limiting the descending height of the chip valve pressure lever; and the liquid path and gas path module is also provided with a liquid path and gas path limiting optocoupler and a liquid path and gas path limiting baffle for limiting the descending height of the annular support.
17. The time-resolved fluoroimmunoassay analyzer of claim 16, wherein the liquid path/gas path module further comprises a chip code scanner for scanning a two-dimensional code on a chip.
18. The time-resolved fluoroimmunoassay analyzer of claim 1, wherein the cleaning module comprises a U-shaped finger assembly and a cleaning module gas-liquid path assembly; after the incubation reaction of the chip is finished, the U-shaped hand-shifting in the U-shaped hand-shifting assembly shifts the chip to a cleaning position in the cleaning module gas-liquid path assembly for cleaning; and after the chip is cleaned, the U-shaped poking hand re-pokes the chip.
19. The time-resolved fluoroimmunoassay analyzer of claim 18, wherein the U-shaped hand-shifting assembly is provided with a U-shaped hand-shifting control motor, under the control of the U-shaped hand-shifting control motor, the U-shaped hand-shifting driving wheel rotates to drive the U-shaped hand-shifting driven wheel through a U-shaped hand-shifting belt, a U-shaped hand-shifting belt connecting block on the U-shaped hand-shifting belt is connected with a U-shaped hand-shifting block assembly, and the U-shaped hand-shifting block assembly is connected with the U-shaped hand-shifting to move along a U-shaped hand-shifting slide rail.
20. The time-resolved fluoroimmunoassay analyzer of claim 19, wherein the U-shaped paddle arm extends to two sides to form a first U-shaped paddle arm and a second U-shaped paddle arm, respectively, and a distance between the first U-shaped paddle arm and the second U-shaped paddle arm is greater than a length of the chip.
21. The time-resolved fluoroimmunoassay analyzer of claim 18, wherein the cleaning module gas-liquid path assembly has a cleaning module control motor, the cleaning module control motor is activated, a cleaning module lead screw is rotated, and a cleaning module motor nut on the cleaning module lead screw drives the cleaning module ring support to move up and down; the cleaning module annular bracket is fixedly connected with a cleaning module slide rail, and the cleaning module slide rail is connected with a cleaning module slide block in a sliding manner; the cleaning module annular support is provided with a cleaning module liquid detection rod and a cleaning module gas-liquid path interface, the cleaning module gas-liquid path interface is respectively a cleaning liquid interface and an external gas path interface, and the cleaning liquid interface and the external gas path interface correspond to a cleaning external gas-liquid port sealing joint at the bottom of the cleaning module annular support; the cleaning module liquid detection rod is connected with the cleaning module liquid detection sensor.
22. The time-resolved fluoroimmunoassay analyzer of claim 21, wherein a cleaning module limiting optocoupler and a cleaning module limiting baffle are further disposed in the cleaning module gas-liquid path assembly for limiting a height of the cleaning module annular frame from descending; the cleaning module ring support is characterized in that a cleaning module chip sample inlet sealing joint is further arranged at the bottom of the cleaning module ring support and is located on the side face of the cleaning module external gas-liquid port sealing joint.
23. The time-resolved fluoroimmunoassay analyzer of claim 1, wherein the chip detection module comprises an optical path detection component and a detection hand-dialing component; a detection shifting handle in the detection shifting handle component shifts the chip to a detection position, the detection position is positioned on a chip carrying platform, and the chip carrying platform moves to enable the optical path detection component to detect the chip; the chip detection module is provided with an upper shielding case and a lower shielding case.
24. The time-resolved fluoroimmunoassay analyzer of claim 23, wherein the detection finger assembly has a detection finger control motor, the detection finger control motor drives the detection finger control motor driving wheel to rotate, the detection finger control motor driving wheel drives the detection finger control motor driven wheel through the detection finger control motor belt; the detection hand-shifting block is connected with a detection hand-shifting block connecting block, the detection hand-shifting block connecting block is fixedly connected with a detection hand-shifting block, and the detection hand-shifting block move along a detection hand-shifting sliding rail.
25. The time-resolved fluoroimmunoassay analyzer of claim 24, wherein one end of the detection hand-piece is provided with a detection hand-piece limiting piece, and a detection hand-piece limiting optical coupler connecting block arranged on a bottom plate of the chip detection module is fixedly connected with a detection hand-piece limiting optical coupler for limiting the motion of the detection hand-piece.
26. The time-resolved fluoroimmunoassay analyzer of claim 23, wherein the optical path detecting unit has a light collecting head, and an emitting plate is disposed on the light collecting head; and a receiving plate is arranged at the lower part of the detection position, the transmitting plate transmits laser, reactants in a chip reaction cavity on the detection position are subjected to fluorescence excitation through the lighting head, then are received by the receiving plate, and a detection result is obtained by calculation after fluorescence collection and amplification.
27. The time-resolved fluoroimmunoassay analyzer of claim 26, wherein a first filter, a first lens, a second lens and a second filter are sequentially disposed from top to bottom below the emission plate; the receiving plate is arranged below the second optical filter; wherein the first filter and the first lens are part of the light collection head; the light collecting head is connected with a light collecting head fixing block, and the light collecting head fixing block is connected with a bottom plate of the chip detection module.
28. The time-resolved fluoroimmunoassay analyzer of claim 27, wherein the second lens and the second filter are provided with a first light-shielding block and a second light-shielding block at side surfaces thereof, respectively; the second light shielding block shields ambient light from the chip discarding channel and serves as a connecting block of the chip discarding channel.
29. The time-resolved fluoroimmunoassay analyzer of claim 23, wherein the stage is driven by a stage control motor, the stage control motor driving a stage screw to rotate, the stage motor nut connecting block on the stage screw moving; the chip carrier motor nut connecting block is connected with the chip carrier, and the chip carrier is connected with a chip carrier sliding block I and a chip carrier sliding block II; the first chip carrier sliding block and the second chip carrier sliding block drive the chip carrier to move along the chip carrier sliding rail.
30. The time-resolved fluoroimmunoassay analyzer of claim 23, wherein the chip detection module is further provided with a discarding pusher for pushing the detected chip into the chip discarding channel; the abandoned shifting handle is driven by a motor controlled by the abandoned shifting handle, a sliding block of the abandoned shifting handle is fixed on a top plate of the chip detection module, and a sliding rail of the abandoned shifting handle is connected with a nut connecting block of a motor of the abandoned shifting handle; the disposal shifting handle control motor drives a disposal shifting handle screw rod to rotate, the disposal shifting handle motor nut connecting block moves along the disposal shifting handle screw rod, the disposal shifting handle motor nut connecting block is connected with a vertical plate, and the vertical plate is connected with a transverse plate; one end of the abandoning shifting handle is connected with the transverse plate to serve as a abandoning shifting handle fixing part, and the other end of the abandoning shifting handle serves as a abandoning shifting handle shifting part and is used for shifting a chip to the chip abandoning channel.
31. The time-resolved fluoroimmunoassay analyzer of claim 30, wherein a disposal handle stopper is fixed on the nut connection block of the disposal handle motor, and a disposal handle sensing portion of the disposal handle stopper cooperates with a disposal handle stopper sensor installed on the top plate for limiting the disposal handle.
32. The time-resolved fluoroimmunoassay analyzer of claim 31, wherein two disposal fingers are provided, one on each side of the horizontal plate.
33. The time-resolved fluoroimmunoassay analyzer of any one of claims 1 to 32, wherein the time-resolved fluoroimmunoassay analyzer is further coated with a housing on the outside, and the housing is provided with a display screen; the shell is provided with a sample inlet for inserting a chip; a disposal groove is also formed in the side surface of the shell; the time-resolved fluorescence immunoassay analyzer is also provided with a built-in printer, and a paper outlet of the built-in printer is arranged on the shell.
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| CN202010444390.7A CN111487400A (en) | 2020-05-22 | 2020-05-22 | Time-resolved fluorescence immunoassay analyzer |
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| CN202010444390.7A CN111487400A (en) | 2020-05-22 | 2020-05-22 | Time-resolved fluorescence immunoassay analyzer |
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