CN208526655U - A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it - Google Patents
A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it Download PDFInfo
- Publication number
- CN208526655U CN208526655U CN201820620734.3U CN201820620734U CN208526655U CN 208526655 U CN208526655 U CN 208526655U CN 201820620734 U CN201820620734 U CN 201820620734U CN 208526655 U CN208526655 U CN 208526655U
- Authority
- CN
- China
- Prior art keywords
- liquid
- area
- chemiluminescence
- fluidic chip
- inlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of chemiluminescence micro-fluidic chips comprising chip body and the injection port being arranged in the chip body, liquid driven power entrance, substrate luminescent solution entrance, filter washing water inlet, substrate luminescent solution subchannel, cleaning solution subchannel, main fluid passageway and multiple functional areas；The main fluid passageway is connected to the multiple functional areas.The chemiluminescence micro-fluidic chip of the utility model realizes that the identification of fluid sample positions and quantitatively, reduces the manufacture craft difficulty of chip, improve the accuracy of detection by specific liquid quantitative area.
Description
Technical field
The utility model relates to medical instruments field more particularly to a kind of chemiluminescence micro-fluidic chip and with its point
Analyzer device.
Background technique
In-vitro diagnosis (In Vitro Diagnosis, IVD) refers to taking-up sample (blood, body fluid, the tissue from human body
Deng) test and analyze to being diagnosed to disease, need corresponding instrument and reagent in detection process, and these instruments and
Reagent just constitutes extracorporeal diagnostic system.The system of in-vitro diagnosis is roughly divided into two kinds；One is be with inspection center laboratory
Represent, it have system modular, automation, the carry out Sample of pipeline system, thus also have high throughput, high efficiency,
The advantage of high sensitive, but whole system also has somewhat expensive, shared volume is big, the defect for needing professional to operate, it
It is mainly used in large hospital.Another be with detect immediately (point-of-care testing, POCT) be representative, it
System have it is integrated, miniaturization, carry out Sample whenever and wherever possible, thus also have price material benefit, it is easy to operate, as a result
Report timely advantage, but its test result compares that there is also sensitivity, the not high disadvantages of stability with central laboratory.
For POCT, has both at home and abroad and microflow control technique is applied in the product of in-vitro diagnosis.It is micro-fluidic
It (microfluidics) is a cross discipline for carrying out control operation to microfluid on one piece of chip with microchannel,
It is related to the fields such as biology, chemistry, fluid physics, electronics, optics, mechanical engineering.Micro fluidic device is commonly known as micro-fluidic
Chip, also referred to as chip lab (Lab on a Chip).Usually biology, chemical, medical analysis process sample system
The basic operations such as standby, reaction, separation, detection are concentrated on one chip, and a system function is completed.Existing micro-fluidic chip
Mainly based on qualitative detection, the micro-fluidic chip of quantitative detection is less, and existing quantitative micro-fluidic chip preparation is complicated, raw
Low efficiency is produced, " a kind of magnetic microparticle chemiluminescence is micro-fluidic as the Chinese patent application of Publication No. " CN105214744 " discloses
Chip ", affiliated micro-fluidic chip include top plate and bottom plate, wherein affiliated top plate includes air pump, adding mouth, sample fill area, mark
Remember ligand storage pool and sample mixed zone；The bottom plate includes filtering area, magnetic particle coating area, cleaning area, detection zone, cleaning solution
Storage pool, luminous substrate liquid storage pool and liquid release channel；The top plate and bottom plate all include liquid sensing device, are used to true
Determine the flow regime of liquid in micro-fluidic chip and whether be mixed into bubble etc., the chip in this patent uses multilayered structure, and
It uses the bag container of specific volume to realize quantifying for liquid, although such D-M (Determiner-Measure) construction is simple, bag container surface is easily
Liquid hanging bag phenomenon occur, (when extruding liquid from bag container, partially liq extension is stayed in bag, not can guarantee liquid is complete
Portion extrudes), and the deflection that bag container is squeezed every time is all different, so each amount of liquid remained in bag container is not
Unanimously, and then the amount of liquid that is extruded of liquid is different, and when in particular for gobbet, the error of bag container is more
Greatly, relative to micro-fluidic chip, what is needed is all tens microlitres of amount, so the quantitative accurate degree of bag container is unable to reach and wants
It asks, dosing accuracy is poor, to influence testing result, while bag container needs to be built into chip, increases the life of chip
Produce difficulty.
Utility model content
To solve the above problems, one side the utility model provides a kind of chemiluminescence micro-fluidic chip, it can be real
Now accurate quantitative detection, and structure is simple, reduces the manufacture craft difficulty of chip.
A kind of the technical solution adopted in the utility model are as follows: chemiluminescence micro-fluidic chip comprising chip body and
Injection port, liquid driven power entrance, substrate luminescent solution entrance, filter washing water inlet, substrate hair in the chip body are set
Light liquid subchannel, cleaning solution subchannel, main fluid passageway and multiple functional areas；The main fluid passageway is connected to the multiple function
Area；
The functional areas include antibody embedding area and the chemiluminescence detection area positioned at antibody embedding area downstream；Its
In, enzyme mark primary antibody and magnetic mark secondary antibody are embedded in antibody embedding area, antibody embedding area is also liquid quantitative area；
The injection port is connected to the main fluid passageway respectively with the liquid driven power entrance, the liquid driven power
Entrance is for connecting liquid driving device to drive liquid to flow in or out the functional areas；Substrate luminescent solution subchannel
One end is connected to the substrate luminescent solution entrance, and the other end is connected to the inlet in antibody embedding area, substrate luminescent solution
Enter antibody embedding area through the substrate luminescent solution entrance, substrate luminescent solution subchannel to be quantified；The cleaning solution branch
The one end in channel is connected to the filter washing water inlet, and the other end is connected to the inlet in antibody embedding area, cleaning solution warp
The filter washing water inlet, cleaning solution subchannel enter antibody embedding area and carry out magnetic bead cleaning.
The liquid driving device is plunger pump in one of the embodiments,.
Dilution entrance and dilution subchannel are additionally provided in the chip body in one of the embodiments,；It is described
One end of dilution subchannel is connected to the dilution entrance, and the other end is connected to the inlet in antibody embedding area,
Sample diluting liquid enters antibody embedding area through the dilution entrance, dilution subchannel and is quantified.
The functional areas further include sample amounts area in one of the embodiments, and the sample amounts chamber is also liquid
Body quantification area, fluid sample flow into the sample amounts area through injection port and are quantified；The sample amounts area is located at described anti-
The upstream in body embedding area；
The air subchannel for being additionally provided with air intake on the chemiluminescence micro-fluidic chip and communicating therewith, the air
One end of subchannel is connected to the air intake, the mainstream between the other end and the sample amounts area and the injection port
The connection of body channel, the other end of the air subchannel and the connectivity part of the main fluid passageway are adjacent to the sample amounts area.
The liquid quantitative area has scheduled volume in one of the embodiments, and in the liquid quantitative area
Liquid identification site is provided at liquid outlet, the liquid that need to be quantified, which flows into the liquid from the inlet in the liquid quantitative area, to be determined
Area is measured, full of reaching the liquid outlet behind the liquid quantitative area.
Liquid identification site is also equipped at the inlet in the liquid quantitative area in one of the embodiments,.
The chemiluminescence detection area has scheduled volume in one of the embodiments, and in the chemiluminescence
Liquid identification site, inlet stream of the liquid to be detected through the chemiluminescence detection area are provided at the liquid outlet of detection zone
Enter the chemiluminescence detection area, full of reaching liquid outlet behind the chemiluminescence detection area, the chemiluminescence detection area
Volume is less than or equal to the volume in magnetic mark secondary antibody embedding area.
Liquid identification site is also equipped at the inlet in the chemiluminescence detection area in one of the embodiments,；
The volume in the chemiluminescence detection area is equal to the volume in magnetic mark secondary antibody embedding area.
The Liquid identification site is for positioning Liquid identification device in one of the embodiments,；The Liquid identification
Device includes light source generation module and photoelectric sensor；
The Liquid identification site includes upper site for positioning the light source generation module and for positioning the light
The lower site of electric inductor, the upper site and the lower site are respectively arranged on the outside of the chip body, the upper site
It is corresponding with the position in the lower site and corresponding liquid outlet or inlet so that positioning after the light source generation module,
Corresponding liquid outlet or inlet, the perpendicular line of the photoelectric sensor are successively laid.
The liquid quantitative area is hexagonal structure in one of the embodiments,.
The width of the inlet in the liquid quantitative area is 0.3-3mm in one of the embodiments, is highly 0.3-
3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-3mm, is highly 0.3-3mm；Or
The surface in the liquid quantitative area is the surface formed after hydrophilic surface is modified；The liquid quantitative area
The width of inlet is 0.3-5mm, is highly 0.3-3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-5mm, high
Degree is 0.3-3mm；Or the surface in the liquid quantitative area is the surface formed after hydrophobic surface is modified, the liquid is fixed
The width for measuring the inlet in area is 0.3-2mm, is highly 0.3-3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-
2mm is highly 0.3-3mm.
The chip body includes top plate and bottom plate in one of the embodiments,；The top plate and the bottom plate are laminated
Connection；The bottom plate is smooth plate, is provided with micropore, microchannel or microcavity body on the top plate to be formed with the bottom plate
The injection port, liquid driven power entrance, substrate luminescent solution entrance, filter washing water inlet, substrate luminescent solution subchannel, cleaning solution branch
Channel, main fluid passageway or functional areas.
Whole Blood Filtration area is equipped between the injection port and the sample amounts area in one of the embodiments, it is described
Whole blood filter membrane is equipped in Whole Blood Filtration area.
One is respectively laid for phase magnet above and below antibody embedding area in one of the embodiments,
Magnet site, the diagonally opposing corner that two magnet sites correspond to antibody embedding area are laid.
It is equipped with to mix between the antibody embedding area and the chemiluminescence detection area in one of the embodiments, and lead to
Road.
It is logical to be respectively arranged on the main fluid for the injection port and the liquid driven power entrance in one of the embodiments,
The both ends in road.
The feed liquor in the other end Yu the antibody embedding area of substrate luminescent solution subchannel in one of the embodiments,
The connectivity part of mouth is located in the main fluid passageway neighbouring with the inlet.
The cleaning solution enters described anti-through the filter washing water inlet, cleaning solution subchannel in one of the embodiments,
Body embedding area is quantified；The other end of the cleaning solution subchannel and the connectivity part of the inlet in antibody embedding area are located at
With the inlet in neighbouring main fluid passageway.
On the other hand, the utility model additionally provides a kind of analysis instrument with chemiluminescence micro-fluidic chip, packet
Include apparatus frame, substrate luminescent solution storage pool, cleaning solution storage pool, liquid driving device, magnet, Liquid identification device, detection
Device and above-described chemiluminescence micro-fluidic chip；Wherein, the chemiluminescence micro-fluidic chip is mounted on the instrument
In frame；The liquid driving device is connected with the liquid driven power entrance of the chemiluminescence micro-fluidic chip, the substrate
Luminescent solution storage pool can be connected to on-off with the substrate luminescent solution entrance, the cleaning solution storage pool and the filter washing water inlet
It can be connected to on-off；The detection device is for receiving the chemiluminescence signal for handling the chemiluminescence detection area；The liquid
Body identification device is located at the Liquid identification site, and the position of the magnet is corresponding with magnetic mark secondary antibody embedding area.
The liquid driving device is plunger pump in one of the embodiments,；The substrate luminescent solution storage pool, cleaning
The opening being connected to outside air is respectively equipped on liquid storage pool.
Compared with the existing technology, the utility model has the following beneficial effects:
The chemiluminescence micro-fluidic chip of the utility model by specific liquid quantitative area in conjunction with liquid driving device come
Realize that the identification of liquid positions and quantitatively, improves quantitative accuracy；
The chemiluminescence microfluidic chip structure of the utility model is compact, such as antibody embedding area is applied not only to be embedded with enzyme
Primary antibody and magnetic mark secondary antibody are marked, can also be used in quantifying substances luminescent solution as liquid quantitative area, and need not liquid be separately set again
Quantification area, the magnetic mark secondary antibody embedding Qu Haike is further used as the area cleaned for magnetic bead, and magnetic bead need not separately be arranged again
The volume of chip is greatly saved in cleaning area；Meanwhile (such as quantifying substances luminescent solution storage pool, cleaning solution store reagent storage pool
Pond etc.) external to chip, reagent plates in chip compared with the existing technology, the manufacture craft difficulty of chip is reduced,
Improve the accuracy of detection.
The chip body of the chemiluminescence micro-fluidic chip of the utility model may include the top plate and bottom plate being stacked, and need
On the settable top plate of the structure to be completed the process, bottom plate is only smooth plate, can further decrease the system of chip in this way
Make technology difficulty, improves production efficiency.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of embodiment of chemiluminescence micro-fluidic chip provided by the utility model；
Fig. 2 is the schematic cross-section of Liquid identification device provided by the utility model；
Fig. 3 is a kind of arrangement structure for sensor of embodiment of chemiluminescence micro-fluidic chip provided by the utility model
Figure；
Fig. 4 is that the section of magnet setting position when chemiluminescence micro-fluidic chip provided by the utility model uses is illustrated
Figure；
Fig. 5 is a kind of structural schematic diagram of embodiment of liquid driving device provided by the utility model；
Wherein, 100, top plate；200, bottom plate；101, injection port；102, Whole Blood Filtration area；103, sample amounts area；104,
Antibody embeds area；105, channel is mixed；106, chemiluminescence detection area；107, substrate luminescent solution entrance；108, dilution entrance；
109, filter washing water inlet；110, liquid driven power entrance；111, air intake；112, substrate luminescent solution subchannel；113, it dilutes
Liquid subchannel；114, cleaning solution subchannel；115, main fluid passageway；116, air subchannel；117, substrate luminescent solution storage pool；
118, dilution storage pool；119, cleaning solution storage pool；120, plunger pump；1201, the inlet of pump chamber；1202, pump chamber go out
Liquid mouth；1203, plunger；121, waste liquid pool；301, light source generation module；302, photoelectric sensor；401,402, magnet；403, magnetic
Pearl.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
Every other embodiment obtained, fall within the protection scope of the utility model.
Embodiment 1
Please refer to FIG. 1 to FIG. 5, present embodiments provide a kind of chemiluminescence micro-fluidic chip comprising chip body, with
And injection port 101, liquid driven power entrance 110, substrate luminescent solution entrance 107, filter washing water inlet in chip body are set
109, substrate luminescent solution subchannel 112, cleaning solution subchannel 114, main fluid passageway 115 and multiple functional areas；It carries out below detailed
It describes in detail bright.
In the present embodiment, main fluid passageway 115 is connected to multiple functional areas, to guide stream of the fluid between functional areas
It is dynamic.
Functional areas include antibody embedding area 104 and and positioned at antibody embedding 104 downstream of area chemiluminescence detection area 106.
Wherein, enzyme mark primary antibody and magnetic mark secondary antibody are embedded in antibody embedding area 104；It is also fixed for liquid that antibody embeds area 104
Measure area.Liquid quantitative area is used for quantitative liquid, can after quantitative liquid (such as substrate luminescent solution) enters liquid quantitative area
Realized in liquid quantitative area quantitative (obtaining desired amount of liquid), with quantitative fluid sample or other reaction reagents
Reaction, to realize quantitative detection.
Chemiluminescence detection area is for accommodating chemiluminescence reaction product, to complete to detect in conjunction with external detection device
Journey.
In the present embodiment, injection port 101 is connected to main fluid passageway 115 respectively with liquid driven power entrance 110, liquid
Body driving force entrance 110 drives liquid inflow or downstream area for connecting liquid driving device；Injection port 101 is used for will
Fluid sample is introduced into main fluid passageway 115, and fluid sample enters each functional areas through main fluid passageway 115.
In the present embodiment, one end of substrate luminescent solution subchannel 112 is connected to substrate luminescent solution entrance 107, another
It holds and is connected to the inlet in antibody embedding area 104, substrate luminescent solution is through substrate luminescent solution entrance 107, substrate luminescent solution subchannel
112, which enter antibody embedding area 104, is quantified.
One end of cleaning solution subchannel 114 is connected to filter washing water inlet 109, the other end and antibody embedding area 104 into
The connection of liquid mouth, the cleaned liquid entrance of cleaning solution, cleaning solution subchannel enter antibody embedding area and carry out magnetic bead cleaning.
The micro-fluidic chip of the present embodiment in use, substrate luminescent solution entrance 107, filter washing water inlet 109 respectively with substrate
Luminescent solution storage pool 117, cleaning solution storage pool 119 can be connected to on-off by valve, substrate luminescent solution storage pool 117, cleaning
The opening being connected to outside air is respectively equipped on liquid storage pool 119；Liquid driving device is mounted on liquid driven power entrance 110
Place, flows to liquid in driving chip；The outside in antibody embedding area 104 is fixed with magnet (such as magnet 401,402), with
Just magnetic bead 403 is fixed.It is liquid quantitative area that antibody, which embeds area 104, can be used for quantifying substances luminescent solution, optionally, can also be into
One step is used for quantitative cleaning solution.
The fluid sample (serum or blood plasma such as after diluted) of predetermined amount is under the action of liquid driving device
It is flow to antibody embedding area 104 through main fluid passageway 115 from injection port, is mixed with the enzyme mark primary antibody and magnetic mark secondary antibody wherein embedded
Reaction forms the reactant of double antibodies sandwich structure on magnetic bead, and magnetic bead is adsorbed by magnet, and reactant is stablized under the action of magnetic bead
In antibody embedding area 104, and remaining reaction solution is discharged under the action of liquid driving device through liquid driven power entrance 110
Chip；Then, air stream inbound port (such as sample inlet) on chip is closed, cleaning solution storage pool 119 and filter washing water inlet are opened
Valve between 109, cleaning solution cleaned liquid subchannel 114 under the action of liquid driving device enter antibody and embed area 104
To be cleaned to magnetic bead therein, when antibody embedding area 104 complete to cleaning solution it is quantitative when, can close cleaning solution storage
Valve between pond 119 and filter washing water inlet 109 opens air inflow end mouth, and the liquid after cleaning is in liquid driving device
Chip is discharged through liquid driven power entrance 110 under effect can clean (magnetic bead cleaning side for several times to guarantee cleaning effect repeatedly
Formula is not limited to mode described herein, can also for example, by cleaning solution the mode of moving magnet realize the cleaning of magnetic bead)；
The air inflow end mouth (such as sample inlet) being then switched off on chip opens substrate luminescent solution storage pool 117 and substrate luminescent solution
Valve between entrance 107, substrate luminescent solution enter under the action of liquid driving device through substrate luminescent solution subchannel 112 anti-
Body embed area 104, when antibody embedding area 104 complete to substrate luminescent solution it is quantitative when, close substrate luminescent solution storage pool 117 with
Valve between substrate luminescent solution entrance 107, liquid driving device stop driving effect, and substrate luminescent solution is no longer flow into antibody packet
Buried district 104, opens the air inflow end mouth (such as sample inlet) on chip, it is quantitative after substrate luminescent solution and magnetic capture it is anti-
It answers object to carry out luminescence-producing reaction, removes magnet later, the reaction solution in antibody embedding area 104 flows under the action of liquid driving device
Enter chemiluminescence detection area 106 to be detected.
Above-mentioned chemiluminescence microfluidic chip structure is compact, for example, antibody embedding area be applied not only to be embedded with enzyme mark primary antibody and
Magnetic mark secondary antibody can also be used in quantifying substances luminescent solution as liquid quantitative area, and liquid quantitative area need not separately be arranged again, magnetic
Mark secondary antibody embedding Qu Haike is further used as the chamber cleaned for magnetic bead, and magnetic bead cleaning area need not separately be arranged again, significantly
Save the volume of chip；Meanwhile reagent storage pool (such as quantifying substances luminescent solution storage pool, cleaning solution storage pool) can be external
In chip, reagent is plated in chip compared with the existing technology, is reduced the manufacture craft difficulty of chip, is improved detection
Accuracy.
It should be noted that main fluid passageway 115 and multiple functional areas can pass through laser processing, model injection molding etc.
Various ways shape inside chip body, can also by being set as the top plate 100 and bottom plate 200 of separate type, in top plate 100 or
Specific shape is processed on bottom plate 200, is then mutually packaged together；Since former processing method is relatively complicated, at one
In preferred embodiment, chip body includes top plate 100 and bottom plate 200；Top plate 100 and the stacking of bottom plate 200 connect；Top plate 100
The junction of bottom plate 200 is provided with main fluid passageway 115 and multiple functional areas；It is highly preferred that bottom plate 200 is smooth puts down
Plate, be arranged on top plate 100 micropore, microchannel or microcavity body to cooperatively form injection port 101 with bottom plate 200, liquid driven power enters
Mouth 110, substrate luminescent solution entrance 107, filter washing water inlet 109, substrate luminescent solution subchannel 112, cleaning solution subchannel 114, master
Fluid channel 115 or functional areas, the preparation of such micro-fluidic chip are got up more convenient, further reduced production technology hardly possible
Degree only need to process required specific structure on top plate, further improve production efficiency.Specifically, bottom plate 200 is light
Sliding plate, top plate 100 are equipped with multiple microchannels to form main fluid passageway 115 to be combined with bottom plate 200, set on top plate 100
There are multiple microcavitys to form multiple functional areas to be combined with bottom plate 200, top plate 100 is equipped with multiple micropores to be combined with bottom plate 200
Form injection port 101, liquid driven power entrance 110, substrate luminescent solution entrance 107 and filter washing water inlet 109；For the ease of into
The size of sample, injection port 101 is typically larger than the size of other entrances.
Therefore, the chip body of above-mentioned chemiluminescence micro-fluidic chip may include the top plate and bottom plate being stacked, and need
On the settable top plate of the structure completed the process, bottom plate is only smooth plate, can further decrease the production of chip in this way
Technology difficulty improves production efficiency.
Preferably, liquid quantitative area has scheduled volume, and liquid knowledge is provided at the liquid outlet in liquid quantitative area
Other site, the liquid that need to be quantified reach out behind the inlet influent quantification area in liquid quantitative area, hydraulically full quantification area
Liquid mouth.Liquid identification site is for positioning or fixing liquid identification device, Liquid identification device liquid for identification.When liquid arrives
When at up to liquid outlet, Liquid identification device can provide signal, and liquid quantitative area is full of by indicating liquid, controls liquid at this time and drives
Dynamic device stops driving liquid, realizes liquid quantifying in liquid quantitative area.Chemiluminescence micro-fluidic chip passes through specific
Liquid quantitative area realizes quantifying for liquid in conjunction with liquid driving device, and quantitative accuracy can be improved.
Preferably, the connectivity part of the inlet in the other end with antibody the embedding area 104 of substrate luminescent solution subchannel 112 is located at
In the main fluid passageway of the inlet in neighbouring antibody embedding area 104；In one embodiment, " neighbouring " is interpreted as " distance herein
0.5~the 10mm of inlet (preferably 0.5~2mm) in antibody embedding area 104 ".
Preferably, the cleaned liquid entrance 109 of cleaning solution, cleaning solution subchannel 114 enter antibody embedding area 104 and are determined
Amount；The connectivity part of the inlet in the other end and antibody the embedding area 104 of cleaning solution subchannel 114 is located at the master neighbouring with inlet
On fluid channel；In one embodiment, " neighbouring " is interpreted as " 0.5~10mm of inlet apart from antibody embedding area 104 herein
(preferably 0.5~2mm) ".
Optionally, Liquid identification site is also equipped at the liquid outlet in liquid quantitative area.This Liquid identification site is also used for
Fixed or positioning Liquid identification device, convenient for liquid in chip flowing and bubble that may be present be monitored, control,
It can also be achieved the mixing between two kinds of quantitative liquids, such as fluid sample and reagent (such as reaction reagent, sample processing reagent)
Between mixing.Portion in the chip needs two kinds of liquid contacts to the mixing of two kinds of liquid of realization, and gap is not present in centre,
And the micro-fluidic chip of the utility model is to realize the contact of quantitative and two kinds of liquid of liquid simultaneously, this requires wherein
It is a kind of it is quantitative after liquid rest on pre-position, another liquid preferably begins to flow into liquid quantitative from this predetermined position
Area realizes quantitatively, and at the optimal selection in this predetermined position, that is, liquid quantitative area inlet in liquid quantitative area；In feed liquor
Liquid identification site is set to position Liquid identification device, this Liquid identification device can provide stopping for one of liquid at mouthful
Stay the feed liquor signal of indication signal and another liquid, the cooperation of the Liquid identification device at the liquid outlet in liquid quantitative area
Under, quantitative and two kinds of liquid the contact of liquid can be realized.
Further, dilution entrance 108 and dilution subchannel 113 are additionally provided in chip body；Dilution subchannel
113 one end is connected to dilution entrance 108, and the other end is connected to the inlet in antibody embedding area 104, sample diluting liquid warp
Dilution entrance, dilution subchannel enter antibody embedding area and are quantified.Sample diluting liquid can not only diluent liquid sample
(such as serum, blood plasma), reduces its concentration and viscosity, wherein the substance contained can also reduce the background values of fluid sample, makes
It must detect more accurate, while sample diluting liquid can preferably redissolve enzyme mark primary antibody；In this technical solution, antibody embeds area
104 can also be used in quantitative sample diluting liquid, without realizing quantifying for sample diluting liquid, quantitative sample dilution in chip exterior
Liquid can embed area 104 in antibody and be mixed with quantitative fluid sample, can save manpower, it is more convenient to operate.In use, will
Dilution entrance 108 and dilution storage pool 118 can be connect to on-off by valve V1, dilution storage pool 118 be equipped with
The opening of outside air connection；The fluid sample (serum or blood plasma such as after diluted) of predetermined amount is filled in liquid driven
It flow at the inlet in antibody embedding area 104, closes on chip through main fluid passageway 115 from injection port 101 under the action of setting
Air inflow end mouth (such as sample inlet), open the valve between dilution storage pool 118 and dilution entrance 108, sample
Dilution enters antibody through dilution subchannel 113 under the action of liquid driving device and embeds area 104, when it is full of antibody packet
Buried district 104 is closed between dilution storage pool 118 and dilution entrance 108 when reaching at the liquid outlet in antibody embedding area 104
Valve, open air inflow end mouth (such as sample inlet), fluid sample and sample diluting liquid can be in liquid driving devices
Continue to flow under suction function, and can be in the positive pressure of liquid driving device, negative pressure alternating action in 115 antibody packet of main fluid passageway
Mixing is realized in buried district, can also realize better mixing by the mixing channel 105 of setting certainly.
Optionally, functional areas further include sample amounts area 103, and sample amounts area 103 is also liquid quantitative area, fluid sample
Sample amounts area is flowed into through injection port 101 to be quantified；Sample amounts area 103 is located at the upstream in antibody embedding area 104；It is micro-fluidic
The air subchannel 116 for being additionally provided with air intake 111 on chip and communicating therewith, one end and the air of air subchannel 116 enter
Mouthful connection, the main fluid passageway between the other end and sample amounts area 103 and injection port 101 is connected to, air subchannel 116 it is another
The connectivity part of one end and main fluid passageway is adjacent to sample amounts area 103.In one embodiment, herein " neighbouring " be interpreted as " away from
0.5~10mm of inlet (preferably 0.5~2mm) from sample amounts area 103 ".It, can by being provided with sample amounts area 103
Convenient for quantifying for fluid sample, without separately quantitative outside chip so that chip use it is more convenient.
Sample amounts area 103 is also liquid quantitative area, and specifically, sample amounts area has scheduled volume, and in sample
The Liquid identification site for positioning Liquid identification device is provided at the liquid outlet of quantification area 103, need to liquid quantitatively from sample
The inlet of product quantification area 103 flows into sample amounts area 103, full of reaching liquid outlet behind sample amounts area 103.It is highly preferred that
Liquid identification site is also equipped at the inlet in sample amounts area.
Micro-fluidic chip is in use, air intake 111 and the air pipeline of chip exterior can be connected to on-off by valve
It connects, enters chip interior to control air.Fluid sample is under the action of liquid driving device through injection port from sample amounts area
103 inlet flows into sample amounts area 103, when at the liquid outlet that fluid sample flow to sample amounts area 103, that is, is full of
Sample amounts area 103 is now placed in the Liquid identification device positioned on the Liquid identification site of liquid outlet and issues indication signal, control
Air intake processed is opened, and the driving force as needed for the flowing of air in air subchannel 116 is small, and needed for the flowing of fluid sample
Driving force is bigger, therefore fluid sample rests on air subchannel 116 and the connectivity part of main fluid passageway does not continue to flow into sample
Fluid sample quantifying in sample amounts area 103 can be completed in product quantification area 103.Fluid sample after quantitative can be in liquid
Continue to flow to antibody embedding area under the action of driving device.
Optionally, chemiluminescence detection area 106 has scheduled volume, and the liquid outlet in chemiluminescence detection area 106
Place is provided with Liquid identification site, and liquid to be detected flows into chemiluminescence detection through the inlet in chemiluminescence detection area 106
Area 106, full of liquid outlet is reached behind chemiluminescence detection area 106, the volume in chemiluminescence detection area 106 is less than or equal to antibody packet
The volume of buried district 104.The Liquid identification site being arranged at the liquid outlet in chemiluminescence detection area 106 can be used for positioning Liquid identification
Device, reaction solution after substrate luminescent solution is reacted with the reactant of magnetic capture reach chemiluminescence detection area 106
When at liquid outlet, Liquid identification device issues signal, and liquid driving device controls reaction solution and stops flowing, can be examined at this time
It surveys.Preferably, Liquid identification site is also equipped at the inlet in chemiluminescence detection area 106；Chemiluminescence detection area 106
Volume is equal to the volume in antibody embedding area 104.
The description as described in liquid quantitative area is applicable in liquid quantitative area (including antibody packet in this present embodiment in embodiment 3
Buried district 104 and sample amounts area 103) description, details are not described herein.
Description is applicable in Liquid identification in this present embodiment as described in Liquid identification site and Liquid identification device in embodiment 4
The description of site and Liquid identification device, details are not described herein.
Optionally, for the ease of the mixing between fluid sample, reagent (sample diluting liquid, substrate luminescent solution etc.), antibody
It embeds to be equipped between area 104 and chemiluminescence detection area 106 and mixes channel 105.
As shown in figure 4, optionally, for the ease of fixed magnetic bead 403, chip body position corresponding with antibody embedding area 104
The place of setting is provided with the fixed site of magnet；Further, it is carried out since the cleaning of magnetic bead 403 can embed area 104 in antibody, in order to more
Realize that magnetic bead cleans well, it is solid respectively to lay the magnet for phase magnet 401,402 above and below antibody embedding area
Anchor point, the diagonally opposing corner that two magnet 401,402 correspond to magnetic mark secondary antibody embedding area 104 are laid.
Optionally, liquid driving device is plunger pump 120, and the description as described in plunger pump 120 is suitable for this reality in embodiment 2
Example is applied, details are not described herein.
Optionally, injection port 101 and liquid driven power entrance 110 are respectively arranged on the both ends of main fluid passageway 115.
Optionally, fluid sample is whole blood, and Whole Blood Filtration area 102 is equipped between injection port 101 and sample amounts area 103,
Whole blood filter membrane is equipped in Whole Blood Filtration area 102；When micro-fluidic chip is used for clinical diagnosis, whole blood is common test sample,
Usually require to carry out when detection whole blood separation with by whole blood serum or blood plasma separate, then reacted with reagent；Core
Whole Blood Filtration area 102 is set in piece, is used convenient for detection, while compared to whole blood is first quantified, then carrying out the mode of whole blood separation,
It is equipped with Whole Blood Filtration area 102 between injection port 101 and sample amounts area 103,103 direct quantitative of sample amounts area can be passed through
The dosage of serum or blood plasma, measurement result are more accurate.The material of whole blood filter membrane can be glass fibre, cotton linter fiber, polyester
Fiber, fiber or blend fibre；Optionally, Whole Blood Filtration filter bed with a thickness of 0.2-2.5mm；The absorption speed of Whole Blood Filtration filter bed
Degree is 4-150s/4cm, water imbibition 30-250mg/cm2。
Optionally, the volume of injection port 101 is 5ul-300ul.
Optionally, the liquid outlet in Whole Blood Filtration area 102 is triangle liquid outlet；The area in Whole Blood Filtration area 102 is 30-
300mm2, width 2-20mm, a length of 5-25mm, depth 0.3-3mm, the angle of front end triangle is 15-160 DEG C.
Optionally, the volume in sample amounts area 103 is 1-50ul.
Optionally, the width for mixing pipeline 105 is 200-2000um, a length of 5mm-40mm, depth 0.2-3mm.
Optionally, the volume in chemiluminescence detection area 106 is 10-200ul.
Next, describing a kind of micro-fluidic chip of embodiment according to the present utility model in conjunction with Fig. 1, Fig. 3 and Fig. 5
Detection method.The method comprising the steps of 101 to step 110, and each step is specific as follows:
Step 101: will respectively with dilution storage pool 118, substrate luminescent solution storage pool 117, cleaning solution storage pool 119,
Plunger pump 120, air communication steel needle insertion chip in closed pad, wherein steel needle respectively with dilution entrance 108, substrate send out
Light liquid entrance 107, filter washing water inlet 109, liquid driven power entrance 110, air intake 111 connect；Whole blood sample is added to
Injection port 101 opens solenoid valve V4 and generates negative-pressure sucking by plunger pump 120, whole blood sample is sucked Whole Blood Filtration area 102.
Step 102: whole blood sample completes filtered serum and is inhaled into sample amounts area 103, and by sample amounts area
The photoelectric sensor (a1, a2) being arranged on 103 inlets and liquid outlet completes the quantitative measurment of serum.
When whole blood sample is by being that inductor output voltage value changes above photoelectric sensor a1, to system one
Identification signal judges the flow locations of liquid in the chips.When sample passes through photoelectric sensor a2, judgement sample determines sample
Amount area 103 is full of, and the intrinsic volume in the region is the quantitative values of sample.
Step 103: blocking injection port 101 and open solenoid valve V5, so that serum is inhaled into antibody embedding area 104.
Step 104: when the photoelectric sensor (b1) being arranged on the inlet in antibody embedding area 104 detects serum, closing
Solenoid valve V5 is closed, solenoid valve V1 is opened, so that external sample dilution enters antibody embedding area 104 from solenoid valve V1.
Step 105: when the photoelectric sensor (b2) being arranged on antibody embedding 104 liquid outlet of area detects that external sample dilutes
When liquid, solenoid valve V1 is closed, solenoid valve V5 is opened, and positive pressure and negative-pressure sucking are sequentially generated by plunger pump 120, so that blood
Clearly, external dilution liquid, the enzyme mark primary antibody embedded in advance, magnetic mark secondary antibody embed area 104 in antibody and mix between pipeline 105 back and forth
Flowing is redissolved, and the reactant of formation is captured by magnetic bead, and magnetic bead is adsorbed and stablized in antibody by the magnet in 104 outside of antibody embedding area
It embeds in area 104, chip is discharged through liquid driven power entrance 110 under the negative-pressure sucking of plunger pump 120 in remaining reaction solution；
Step 107: solenoid valve V5 is closed, and opens solenoid valve V3, exterior washings liquid is made to enter antibody embedding area 104,
And the injection that the photoelectric sensor (b1, b2) being arranged on 104 inlet of area and liquid outlet controls cleaning solution is embedded by antibody
Amount.
Step 108: after external cleaning solution and magnetic bead clean repeatedly, magnet 25a, 25b adsorb magnetic bead, pass through plunger pump
120 generate negative-pressure sucking, and the liquid suction after cleaning is discharged in external waste liquid pool 121.
Step 109: closing solenoid valve V3, open solenoid valve V2, external substrate luminescent solution is made to enter the embedding of magnetic mark secondary antibody
Area 7, and pass through the injection rate of photoelectric sensor (c1, c2) control substrate luminescent solution.
Step 110: after substrate luminescent solution is sufficiently reacted with the antigen-antibody on magnetic bead, obtaining reaction solution, reaction solution quilt
Chemiluminescence detection area 106 is transported, to complete chemiluminescence detection；Wherein, 106 inlet of chemiluminescence detection area and out liquid
The photoelectric sensor (c1, c2) being arranged on mouth is used to detect capacity and the position of reaction solution.
Reaction principle in the chemiluminescence micro-fluidic chip of the present embodiment between substance is the same as magnetic particle immunochemiluminescence
Reaction principle, i.e. antigen in sample by and enzyme mark primary antibody (primary antibody is marked with the catalytic groups such as HRP, AP) combine, then with
Magnetic mark secondary antibody (secondary antibody is fixed on magnetic bead), which combines, forms double antibodies sandwich compound, and magnet adsorbs magnetic bead, washes unbonded
Antigen and enzyme mark primary antibody, substrate reactions liquid is added, the enzymes group catalysis substrate reaction solution such as HRP, AP marked on primary antibody shines.
Luminous intensity and the amount of antigen are directly proportional.
Embodiment 2
Referring to FIG. 5, the utility model provides the liquid driving device of function described in achievable embodiment 1.At this
In embodiment, liquid driving device is plunger pump 120.
For structure, liquid driving device may be configured as a variety of, such as existing syringe pump, diaphragm pump, peristaltic pump, all
It is that by and liquid is driven to the presumptive area to chip under pressure, the protection model of the utility model should all be fallen into
It encloses.Although syringe pump, diaphragm pump, peristaltic pump can drive liquid to flow, they cannot control liquid in certain bits well
Stop is set, and plunger pump can preferably solve this problem.Plunger pump suitable for the utility model can be art technology
Plunger pump known to personnel, generally includes pump chamber and plunger, and pump chamber is equipped with inlet 1201 and liquid outlet 1202, plunger
In 1203 top insertion pump chamber, plunger 1203 is reciprocating in its axial direction along the inner wall of pump chamber；Inlet 1201 goes out
Valve V4, V6 are respectively equipped at liquid mouth 1202.Since plunger pump is applied to imbibition, drain, two be arranged on pump chamber by more
A mouth is commonly known as " inlet and liquid outlet ", but it should be recognized that " inlet and liquid outlet " herein is not limited to
For feed liquor and out liquid, in the present embodiment, when plunger pump work, after the valve V4 at inlet 1201 is opened, plunger is downward
Movement, the pressure that liquid closes on one end of plunger pump inlet 1201 at this time become smaller, and liquid both ends is caused to generate pressure difference, liquid
Body moves under the action of pressure difference to 1201 direction of inlet, when liquid reaches pre-position, opens at liquid outlet
Valve V6, so that chip interior is connected to outside atmosphere, in two sides air, (wherein the air of side is through going out liquid respectively for liquid two sides
Mouth, inlet enter chip interior, and the air duct that the air of the other side can be arranged from injection port or separately enters chip interior)
Under the action of, pressure keeps balance, and liquid can rest on pre-position.
Embodiment 3
Fig. 1 and Fig. 3 are please referred to, the utility model provides the liquid quantitative area of function described in achievable embodiment 1.
It should be noted that the liquid quantitative area of the utility model can be realized " need to liquid quantitatively from liquid quantitative area
Inlet influent quantification area, reach liquid outlet behind hydraulically full quantification area ", shape and structure can as needed into
Row selection, the utility model impose any restrictions not to this, such as it can be pipe shape, polygonal shape etc..
In the present embodiment, liquid quantitative area is hexagonal structure.Optionally, the inlet and liquid outlet in liquid quantitative area
Respectively two of hexagonal structure diagonal；Two diagonal angles are less than 120 °.
Optionally, the width of the inlet in liquid quantitative area is 0.3-3mm (preferably 0.8-1.5mm), is highly 0.3-
3mm；The width of the liquid outlet in liquid quantitative area is 0.3-3mm (preferably 0.8-1.5mm), is highly 0.3-3mm.Feed liquor mouth width
Spend wide or narrow, excessive height or it is too low be unfavorable for quantitative progress, when inlet width is wide or excessive height, hold
Easily cause liquid can not hydraulically full quantification area flow to its liquid outlet, cannot achieve accurate liquid quantitative in this way, and when into
When liquid mouth width spends narrow or highly too low, then needs to increase accordingly requirement of the length to meet volume, may result in core in this way
The increase of leaf length and the increase of chip volume.
Optionally, the surface in liquid quantitative area is the surface formed after hydrophilic surface is modified；Liquid quantitative area
The width of inlet is 0.3-5mm, is highly 0.3-3mm；The width of the liquid outlet in liquid quantitative area is 0.3-5mm, is highly
0.3-3mm.Hydrophilic surface modification includes but is not limited to plasma, hydroxylating, carboxylated modification.The surface in liquid quantitative area into
After row Hydrophilic modification, be more advantageous to the filling of liquid in the cavity, at this time can larger fluid quantification area appropriate inlet,
The width of liquid outlet, so as to reduce the length in liquid quantitative area and chip.
Optionally, the surface in liquid quantitative area is the surface formed after hydrophobic surface is modified, liquid quantitative area
The width of inlet is 0.3-2mm, is highly 0.3-3mm；The width of the liquid outlet in liquid quantitative area is 0.3-2mm, is highly
0.3-3mm.Hydrophobically modified includes but is not limited to hydrophobicity physical modification, (such as nanoparticle coating adds for hydrophobic chemical modification
The alkyl etc. of long-chain).The surface in liquid quantitative area can prevent liquid wall built-up after hydrophobic surface is modified, and can guarantee liquid
Body reaches liquid outlet after being full of in liquid quantitative area.
Description above with respect to the structure in liquid quantitative area is equally applicable to sample amounts area.
Embodiment 4
Fig. 2 is referred to, the utility model provides Liquid identification site and the liquid of function described in achievable embodiment 1
Identification device.
It should be noted that Liquid identification site is for positioning or fixing liquid identification device, the utility model is for liquid
The structure of body identification device with no restriction, as long as being able to achieve the identification of liquid.It is special such as Publication No. " 105214744A "
Liquid sensing device disclosed in benefit application can be used as the Liquid identification device of the utility model, but such liquid sensing dress
It is complex to set structure, conductive pin needs to be built into chip interior, and chip preparation difficulty is larger, provides one in the present embodiment
The preferred Liquid identification device of kind.
In the present embodiment, Liquid identification site includes that light source is raw for positioning Liquid identification device, Liquid identification device
At module 301 and photoelectric sensor 302；Liquid identification site includes for the upper site of positioned light source generation module 301 and use
In the lower site of positioning photoelectric sensor 302, upper site and lower site are respectively arranged on the outside of chip body, upper site, corresponding
Inlet or liquid outlet, the perpendicular line in lower site successively lay.Correspondingly, light source generation module 301, corresponding inlet or
Liquid outlet, the perpendicular line of photoelectric sensor 302 are successively laid.Since Liquid identification device can be set to liquid quantitative area or chemistry hair
" corresponding inlet or liquid outlet " at the inlet or liquid outlet in light detection area, therefore herein correspond to liquid quantitative area or
The inlet or liquid outlet in chemiluminescence detection area；For example, when Liquid identification device is arranged in the liquid outlet in antibody embedding area, light
The perpendicular line of liquid outlet, photoelectric sensor that source generation module, antibody embed area is successively laid；When the inlet in antibody embedding area
When Liquid identification device is set, light source generation module, antibody embedding area the perpendicular line of inlet, photoelectric sensor successively cloth
If；When Liquid identification device is arranged in the liquid outlet in sample amounts area, light source generation module, the liquid outlet in sample amounts area, light
The perpendicular line of electric inductor is successively laid.
Such Liquid identification device can be set to outside micro-fluidic chip, convenient for being fixed in instrument, be existed without setting
On chip, the manufacture difficulty of chip is reduced.In use, only light source generation module and photoelectric sensor alignment liquid need to be identified
It places in site.Specifically, chip body includes top plate 100 and bottom plate 200；Top plate 100 and the stacking of bottom plate 200 connect；Top
The junction of plate 100 and bottom plate 200 is provided with main fluid passageway 115 and multiple functional areas；The setting positioning of light source generation module 301
In the surface of the corresponding position of top plate 100 corresponding with the inlet in liquid quantitative area or liquid outlet, photoelectric sensor 302 is fixed
Underface of the position in the corresponding position of bottom plate 200 corresponding with the inlet in liquid quantitative area or liquid outlet.
Light source generation module 301 can provide the module of light source, can be LED, halogen lamp, laser lamp etc..In light source
Irradiation under, since gas, liquid are different to the transmissivity of light and refractive index, the light intensity for being irradiated to photoelectric sensor is different,
Photoelectric sensor can identify gas and liquid, to distinguish whether liquid arrives the point of induction.When liquid flow to inlet
Or when liquid outlet, Liquid identification device can be identified quickly, to control liquid driving device.
Embodiment 5
The embodiments of the present invention additionally provide a kind of analysis instrument with micro-fluidic chip comprising instrument frame
Frame, substrate luminescent solution storage pool, cleaning solution storage pool, liquid driving device, magnet, Liquid identification device, detection device and with
The chemiluminescence micro-fluidic chip of any one of upper embodiment；Wherein, chemiluminescence micro-fluidic chip is mounted in apparatus frame；
Liquid driving device is connected with the liquid driven power entrance of chemiluminescence micro-fluidic chip, and substrate luminescent solution storage pool and substrate are sent out
Light liquid entrance can be connected to on-off, and cleaning solution storage pool can be connected to on-off with filter washing water inlet；Detection device is used for receiving area
The chemiluminescence signal of the luminous detection zone of Physicochemical；Liquid identification device is located at Liquid identification site, the position of magnet with
It is corresponding that magnetic mark secondary antibody embeds area.
Optionally, liquid driving device is plunger pump；Be respectively equipped on substrate luminescent solution storage pool, cleaning solution storage pool with
The opening of outside air connection.
The above is preferred embodiments of the present invention, it is noted that for the ordinary skill of the art
For personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these are improved and profit
Decorations are also considered as the protection scope of the utility model.
Claims (21)
1. a kind of chemiluminescence micro-fluidic chip, which is characterized in that it includes chip body and is arranged in the chip body
On injection port, liquid driven power entrance, substrate luminescent solution entrance, filter washing water inlet, substrate luminescent solution subchannel, cleaning solution branch
Channel, main fluid passageway and multiple functional areas；The main fluid passageway is connected to the multiple functional areas；
The functional areas include antibody embedding area and the chemiluminescence detection area positioned at antibody embedding area downstream；Wherein, institute
It states and is embedded with enzyme mark primary antibody and magnetic mark secondary antibody in antibody embedding area, antibody embedding area is also liquid quantitative area；
The injection port is connected to the main fluid passageway respectively with the liquid driven power entrance, the liquid driven power entrance
For connecting liquid driving device to drive liquid to flow in or out the functional areas；One end of substrate luminescent solution subchannel
It is connected to the substrate luminescent solution entrance, the other end is connected to the inlet in antibody embedding area, and substrate luminescent solution is through institute
State substrate luminescent solution entrance, substrate luminescent solution subchannel enters antibody embedding area and quantified；The cleaning solution subchannel
One end be connected to the filter washing water inlet, the inlet in the other end and the antibody embedding area be connected to, described in cleaning solution passes through
Filter washing water inlet, cleaning solution subchannel enter antibody embedding area and carry out magnetic bead cleaning.
2. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the liquid driving device is plunger
Pump.
3. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that be additionally provided in the chip body dilute
Release liquid entrance and dilution subchannel；One end of the dilution subchannel is connected to the dilution entrance, the other end with
The inlet connection in antibody embedding area, sample diluting liquid enter described anti-through the dilution entrance, dilution subchannel
Body embedding area is quantified.
4. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the functional areas further include that sample is fixed
Area is measured, the sample amounts chamber is also liquid quantitative area, and fluid sample, which flows into the sample amounts area through injection port, to be determined
Amount；The sample amounts area is located at the upstream in antibody embedding area；
The air subchannel for being additionally provided with air intake on the chemiluminescence micro-fluidic chip and communicating therewith, the air branch are logical
The one end in road is connected to the air intake, and the main fluid between the other end and the sample amounts area and the injection port is logical
Road connection, the other end of the air subchannel and the connectivity part of the main fluid passageway are adjacent to the sample amounts area.
5. chemiluminescence micro-fluidic chip according to any one of claims 1 to 4, which is characterized in that the liquid is fixed
Measuring area has scheduled volume, and is provided with Liquid identification site at the liquid outlet in the liquid quantitative area, the liquid that need to be quantified
Body flows into the liquid quantitative area from the inlet in the liquid quantitative area, full of the liquid out of arrival behind the liquid quantitative area
Mouthful.
6. chemiluminescence micro-fluidic chip according to claim 5, which is characterized in that the inlet in the liquid quantitative area
Place is also equipped with Liquid identification site.
7. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the chemiluminescence detection area has
Scheduled volume, and Liquid identification site is provided at the liquid outlet in the chemiluminescence detection area, liquid warp to be detected
The inlet in the chemiluminescence detection area flows into the chemiluminescence detection area, full of reaching behind the chemiluminescence detection area
Liquid outlet, the volume in the chemiluminescence detection area are less than or equal to the volume in magnetic mark secondary antibody embedding area.
8. chemiluminescence micro-fluidic chip according to claim 7, which is characterized in that the chemiluminescence detection area into
Liquid identification site is also equipped at liquid mouth；The volume in the chemiluminescence detection area is equal to the appearance in magnetic mark secondary antibody embedding area
Product.
9. chemiluminescence micro-fluidic chip according to claim 5, which is characterized in that the Liquid identification site is for fixed
Position Liquid identification device；The Liquid identification device includes light source generation module and photoelectric sensor；
The Liquid identification site includes upper site for positioning the light source generation module and for positioning the light inductance
The lower site of device is answered, the upper site and the lower site are respectively arranged on the outside of the chip body, the upper site and institute
Position and the corresponding liquid outlet or inlet for stating lower site are corresponding, so that the light source generation module after positioning, corresponding
Liquid outlet or inlet, the perpendicular line of the photoelectric sensor successively lay.
10. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the liquid quantitative area is six sides
Shape structure.
11. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the feed liquor in the liquid quantitative area
The width of mouth is 0.3-3mm, is highly 0.3-3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-3mm, is highly
0.3-3mm；Or
The surface in the liquid quantitative area is the surface formed after hydrophilic surface is modified；The feed liquor in the liquid quantitative area
The width of mouth is 0.3-5mm, is highly 0.3-3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-5mm, is highly
0.3-3mm；Or
The surface in the liquid quantitative area is the surface formed after hydrophobic surface is modified, the feed liquor in the liquid quantitative area
The width of mouth is 0.3-2mm, is highly 0.3-3mm；The width of the liquid outlet in the liquid quantitative area is 0.3-2mm, is highly
0.3-3mm。
12. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the chip body includes top plate
And bottom plate；The top plate and bottom plate stacking connect；The bottom plate is smooth plate, be provided on the top plate micropore,
Microchannel or microcavity body are to form the injection port, liquid driven power entrance, substrate luminescent solution entrance, cleaning solution with the bottom plate
Entrance, substrate luminescent solution subchannel, cleaning solution subchannel, main fluid passageway or functional areas.
13. chemiluminescence micro-fluidic chip according to claim 4, which is characterized in that the injection port and the sample
It is equipped with Whole Blood Filtration area between quantification area, is equipped with whole blood filter membrane in the Whole Blood Filtration area.
14. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the top in antibody embedding area
The magnet site for phase magnet is respectively laid with lower section, two magnet sites correspond to the oblique right of antibody embedding area
It lays at angle.
15. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that antibody embedding area and described
It is equipped between chemiluminescence detection area and mixes channel.
16. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the injection port and the liquid
Driving force entrance is respectively arranged on the both ends of the main fluid passageway.
17. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that substrate luminescent solution subchannel
The other end and the connectivity part of inlet in antibody embedding area be located in the main fluid passageway neighbouring with the inlet.
18. chemiluminescence micro-fluidic chip according to claim 1, which is characterized in that the cleaning solution is through the cleaning
Liquid entrance, cleaning solution subchannel enter antibody embedding area and are quantified；The other end of the cleaning solution subchannel with it is described
The connectivity part of the inlet in antibody embedding area is located in the main fluid passageway neighbouring with the inlet.
19. the chemiluminescence micro-fluidic chip according to any one of claim 6~8, which is characterized in that the liquid is known
Other site is for positioning Liquid identification device；The Liquid identification device includes light source generation module and photoelectric sensor；
The Liquid identification site includes upper site for positioning the light source generation module and for positioning the light inductance
The lower site of device is answered, the upper site and the lower site are respectively arranged on the outside of the chip body, the upper site and institute
Position and the corresponding liquid outlet or inlet for stating lower site are corresponding, so that the light source generation module after positioning, corresponding
Liquid outlet or inlet, the perpendicular line of the photoelectric sensor successively lay.
20. a kind of analysis instrument with chemiluminescence micro-fluidic chip, which is characterized in that shine including apparatus frame, substrate
Described in liquid storage pool, cleaning solution storage pool, liquid driving device, magnet, Liquid identification device, detection device and claim 1
Chemiluminescence micro-fluidic chip；Wherein, the chemiluminescence micro-fluidic chip is mounted in the apparatus frame；The liquid
Driving device is connected with the liquid driven power entrance of the chemiluminescence micro-fluidic chip, the substrate luminescent solution storage pool and institute
Stating substrate luminescent solution entrance can be connected to on-off, and the cleaning solution storage pool can be connected to on-off with the filter washing water inlet；Institute
Detection device is stated for receiving the chemiluminescence signal for handling the chemiluminescence detection area；The Liquid identification device is located in
At the Liquid identification site, the position of the magnet is corresponding with magnetic mark secondary antibody embedding area.
21. the analysis instrument according to claim 20 with chemiluminescence micro-fluidic chip, which is characterized in that the liquid
Body drive is plunger pump；It is respectively equipped on the substrate luminescent solution storage pool, cleaning solution storage pool and is connected to outside air
Opening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820620734.3U CN208526655U (en) | 2018-04-27 | 2018-04-27 | A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820620734.3U CN208526655U (en) | 2018-04-27 | 2018-04-27 | A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208526655U true CN208526655U (en) | 2019-02-22 |
Family
ID=65395955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820620734.3U Active CN208526655U (en) | 2018-04-27 | 2018-04-27 | A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208526655U (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108704677A (en) * | 2018-04-27 | 2018-10-26 | 广州万孚生物技术股份有限公司 | A kind of micro-fluidic chip and the analytical instrument containing it |
| CN110026257A (en) * | 2019-04-19 | 2019-07-19 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110026256A (en) * | 2019-04-19 | 2019-07-19 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110387313A (en) * | 2019-08-07 | 2019-10-29 | 哈尔滨工业大学 | A kind of magnetophoresis micro-fluidic chip based on bubble mixing |
| WO2019205778A1 (en) * | 2018-04-27 | 2019-10-31 | 广州万孚生物技术股份有限公司 | Chemiluminescence microfluidic chip and analytical instrument having same |
| CN111721818A (en) * | 2020-06-04 | 2020-09-29 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
| WO2022062994A1 (en) * | 2020-09-27 | 2022-03-31 | 青岛海尔电冰箱有限公司 | Microfluidic detection system, control method therefor, and refrigerator |
| CN117949652A (en) * | 2024-03-22 | 2024-04-30 | 北京京东方技术开发有限公司 | Immunodetection chip and application method |
-
2018
- 2018-04-27 CN CN201820620734.3U patent/CN208526655U/en active Active
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108704677A (en) * | 2018-04-27 | 2018-10-26 | 广州万孚生物技术股份有限公司 | A kind of micro-fluidic chip and the analytical instrument containing it |
| WO2019205779A1 (en) * | 2018-04-27 | 2019-10-31 | 广州万孚生物技术股份有限公司 | Micro-fluidic chip and analysis instrument having same |
| WO2019205778A1 (en) * | 2018-04-27 | 2019-10-31 | 广州万孚生物技术股份有限公司 | Chemiluminescence microfluidic chip and analytical instrument having same |
| CN108704677B (en) * | 2018-04-27 | 2024-05-28 | 广州万孚生物技术股份有限公司 | Microfluidic chip and analytical instrument comprising same |
| CN110026257B (en) * | 2019-04-19 | 2022-04-01 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110026257A (en) * | 2019-04-19 | 2019-07-19 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110026256A (en) * | 2019-04-19 | 2019-07-19 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110026256B (en) * | 2019-04-19 | 2022-05-10 | 深圳市亚辉龙生物科技股份有限公司 | Micro-fluidic chip |
| CN110387313A (en) * | 2019-08-07 | 2019-10-29 | 哈尔滨工业大学 | A kind of magnetophoresis micro-fluidic chip based on bubble mixing |
| CN111721818B (en) * | 2020-06-04 | 2022-07-05 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
| CN111721818A (en) * | 2020-06-04 | 2020-09-29 | 南京市食品药品监督检验院 | Self-driven electrochemical detection chip and preparation method thereof |
| WO2022062994A1 (en) * | 2020-09-27 | 2022-03-31 | 青岛海尔电冰箱有限公司 | Microfluidic detection system, control method therefor, and refrigerator |
| CN117949652A (en) * | 2024-03-22 | 2024-04-30 | 北京京东方技术开发有限公司 | Immunodetection chip and application method |
| CN117949652B (en) * | 2024-03-22 | 2024-06-25 | 北京京东方技术开发有限公司 | Immunodetection chip and application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208526655U (en) | A kind of chemiluminescence micro-fluidic chip and the analysis instrument with it | |
| CN108519373A (en) | A kind of chemiluminescence micro-fluidic chip and the analytical instrument containing it | |
| CN208224274U (en) | A kind of micro-fluidic chip and the analysis instrument with the micro-fluidic chip | |
| CN108704677A (en) | A kind of micro-fluidic chip and the analytical instrument containing it | |
| CN209549516U (en) | A kind of micro-fluidic chip and the analysis instrument containing it | |
| CN108761055A (en) | A kind of micro-fluidic chip and the analytical instrument with the micro-fluidic chip | |
| CN208795661U (en) | A kind of chemiluminescence micro-fluidic chip and the analysis instrument containing it | |
| CN108716938A (en) | A kind of liquid quantifying device and its application | |
| CA1310566C (en) | Element and method for performing biological assays accurately, rapidlyand simply | |
| CN107930710A (en) | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system and their application | |
| ES2620233T3 (en) | Immunoassays, methods for carrying out immunoassays, immunoassay kits and method for manufacturing immunoassay kits | |
| JP2008524605A (en) | Cartridge for diagnostic assay | |
| CN209131768U (en) | A kind of liquid quantifying device | |
| CN207786624U (en) | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system | |
| CN207254328U (en) | Combined quantitative detects the micro-fluidic chip and kit of more biomarkers | |
| CN110244036A (en) | A kind of micro-fluidic detection chip and method for immune detection | |
| WO2010119380A1 (en) | Microfluidic device comprising sensor | |
| CN208172010U (en) | Magnetic bead cleaning device and chemiluminescence micro-fluidic chip with it | |
| WO2021068914A1 (en) | Magnetic particle light-emitting double-layer micro-fluidic chip and detection system | |
| WO2021068912A1 (en) | Magnetic particle luminescence micro-fluidic chip for multi-marker detection, and detection device | |
| US20200147611A1 (en) | Microchip for analyzing fluids | |
| JP2005069997A (en) | Method for detecting allergen protein, detecting chip and detecting apparatus | |
| CN109939751B (en) | Microfluidic chip, detection device and detection method for whole blood detection | |
| CN105628660A (en) | Passive microvalve POCT chip | |
| CN212189148U (en) | Microfluidic chip and detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |