CN113770104A - Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer - Google Patents
Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer Download PDFInfo
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- CN113770104A CN113770104A CN202111225036.6A CN202111225036A CN113770104A CN 113770104 A CN113770104 A CN 113770104A CN 202111225036 A CN202111225036 A CN 202111225036A CN 113770104 A CN113770104 A CN 113770104A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
Abstract
The invention is suitable for the technical field of automated analysis, and provides a magnetic cleaning and separating device for a chemiluminescence immunoassay analyzer, which comprises a rack and a mounting plate arranged on the rack; the central turntable is arranged on the mounting plate and can rotate circumferentially relative to the mounting plate; the central turntable is provided with a bearing hole for bearing the reaction cup; cleaning the assembly; the cleaning assembly is used for injecting liquid and absorbing liquid to the reaction cup; the separation component is used for adsorbing the magnetic beads in the reaction cup after uniform mixing; the blending component comprises a sealing mechanism and a blending mechanism; the mixing mechanism is used for driving the reaction cup to rotate; when the reaction cup rotates, the closing mechanism is used for blocking the opening end of the reaction cup; the device optimizes the mixing mechanism, improves the mixing efficiency, ensures the cleaning effect of the magnetic beads in the reaction cup, and ensures the testing efficiency of subsequent tests and the accuracy of test results.
Description
Technical Field
The invention relates to the technical field of automated analysis, in particular to a magnetic cleaning and separating device and method for a chemiluminescence immunoassay analyzer.
Background
In a full-automatic chemiluminescence immunoassay analyzer, magnetic beads refer to colloidal composite materials which can be uniformly dispersed in a certain base solution. Because of the properties of superparamagnetism, higher specific surface area, capability of modifying functional groups and the like. Thus, antigens/antibodies, enzymes, nucleic acids/oligonucleotides, small molecule drugs, etc. are immobilized on their surfaces. The magnetic separation technology is to carry out solid-liquid phase separation on magnetic beads and reaction liquid. In order to ensure the effect of magnetic separation, in the separation process, the reaction solution needs to be fully and uniformly mixed, and the injection solution and the liquid absorption are repeatedly cleaned to remove impurities, so that an accurate test result is obtained in subsequent detection.
At present, the mainstream mixing mode is that a mixing component is arranged above a reaction cup, the reaction cup needs to be grabbed for mixing, and the reaction cup needs to be released after mixing. The reaction liquid cleaning mechanism is complex in structural design, low in automation degree, low in mixing efficiency and poor in cleaning effect, and the testing efficiency of rear-end detection and the accuracy of a testing result are affected.
Disclosure of Invention
The invention provides a magnetic cleaning and separating device and method for a chemiluminescence immunoassay analyzer, aiming at optimizing a uniform mixing structure of the device and improving the uniform mixing efficiency.
In order to realize the purpose, the invention adopts the following technical scheme to realize the purpose:
a magnetic cleaning and separating device for a chemiluminescence immunoassay analyzer comprises a frame and a mounting plate arranged on the frame;
the central turntable is arranged on the mounting plate and can rotate circumferentially relative to the mounting plate, and the axis of the central turntable is perpendicular to the mounting plate; the central turntable is provided with a bearing hole for bearing the reaction cup; the bearing holes are arranged along the circumferential direction of the central turntable;
the cleaning assembly is arranged on the rack or the mounting plate; the cleaning assembly comprises a cleaning needle assembly which is arranged above the bearing hole and is used for injecting and absorbing liquid into the reaction cup;
the separation assembly comprises a magnetic part, and the magnetic part is positioned on the periphery of the central turntable so as to adsorb the magnetic beads in the reaction cup after uniform mixing;
the blending component is arranged on the rack and comprises a sealing mechanism and a blending mechanism; the mixing mechanism drives the reaction cup to rotate; the closing mechanism blocks the opening end of the reaction cup when the reaction cup rotates.
The separating component comprises a magnet fixing frame arranged around the central turntable, a magnetic part is arranged in a circular notch of the magnet fixing frame, and the magnetic part generally uses a magnet with a customized shape; the magnet fixing frame and the reaction cup turntable are concentrically arranged, and each magnet fixing slot position on the magnet fixing frame ensures that the equal-circle central angles on the circumference taking the center of the turntable as the circle center are uniformly distributed. The relative distance between each magnet and the reaction cup at the corresponding station is ensured to be equal. The magnet is close to the reaction cup as far as possible, but does not interfere the rotation of the central turntable. The magnet fixing frame is provided with a screw hole above the mounting position corresponding to the magnet, and the depth of the magnet feeding can be adjusted by utilizing a set screw, so that the distance between the magnet and the reaction cup corresponding to the station is the most appropriate distance.
The central turntable is driven by a turntable motor to rotate, and the central turntable is responsible for transferring the reaction cups containing the magnetic beads to corresponding operation stations; the operation stations comprise a magnetic adsorption station, an absorption and liquid discharge station and a mixing station. The central turntable is concentrically arranged at the upper end of the central rotating shaft, the lower end of the central rotating shaft penetrates through the mounting plate and is connected with a rotating shaft of a turntable motor through a transmission device (the transmission device is a synchronous belt mechanism, but is independent of the synchronous belt mechanism in the driving mechanism), the turntable motor is arranged on the U-shaped supporting plate, and the turntable motor and the central rotating shaft drive the central turntable to rotate through the transmission device.
The cleaning needle assembly comprises a liquid suction needle and a liquid injection needle; the liquid injection needle and the liquid suction needle are arranged on the needle frame in an inner needle and outer needle mode; the needle frame is provided with a groove position for installing the needle fixing end block, the needle fixing end block is used for fixing the inner needle and the outer needle in a tightly holding mode, and the upper and lower positions of the inner needle and the outer needle can be freely adjusted, namely the distance from the inner needle and the outer needle to the reaction cup; the needle frame is arranged on the needle frame fixing seat, and the needle frame fixing seat plays a role in supporting the suspended needle frame; the needle frame fixing seat is connected with a sliding block on the guide rail mechanism, and the guide rail motor drives the needle frame to move up and down through the up-and-down movement of the sliding block, so that the cleaning needle assembly can reach a proper position in the reaction cup. In this embodiment, since the cleaning needle assembly uses the inner and outer needle structure, the liquid suction needle and the liquid injection needle are set to the same station; therefore, a plurality of cleaning stations are reduced, and the purpose of reducing the size of the whole structure is achieved. The liquid injection needle is concentric with the liquid suction needle and wraps the liquid suction needle, and the liquid injection needle is fixedly connected with the liquid suction needle in a welding mode. When the liquid injection needle discharges cleaning liquid, waste liquid stained on the outer wall of the liquid suction needle can be cleaned at the same time, and the waste liquid in the reaction cup after cleaning is pumped away by the liquid suction needle, so that cross contamination is avoided.
Further, the blending mechanism is arranged below the bearing hole and comprises a driving mechanism and a blending cup head; the driving mechanism drives the blending cup head to rotate.
Further, the mixing cup head is in a sleeve shape; when the mixing cup head is contacted with the reaction cup, the mixing cup head covers the lower end of the reaction cup.
Further, the driving mechanism comprises a blending motor, the blending motor is a direct current brushless motor, and a rotating shaft of the blending motor is connected with a driving wheel arranged above the blending substrate through a coupling; the driving wheel is connected with a plurality of driven wheels through belts, and the driven wheels are connected with the bottom end of the mixing cup head through a mixing shaft; the belt also passes through an idler pulley to adjust the degree of tension of the belt; the driving wheel and the driven wheel are both rotatably arranged on the uniformly mixing substrate; the number of the driven wheels is multiple, and one driving wheel drives the driven wheels to rotate simultaneously; each driven wheel drives a mixing cup head fixedly connected with the driven wheel to drag the reaction cup to rotate; therefore, one motor can drive a plurality of reaction cups to rotate, thereby saving the space and the cost for arranging the motor.
Further, the blending cup head is eccentrically connected with the blending shaft; the inner diameter of the cross section of the mixing cup head is larger than the outer diameter of the cross section of the reaction cup; the reaction cup is inserted into the bearing hole through the elastic ring, and a gap exists between the reaction cup and the bearing hole so as to increase the shaking amplitude of the reaction cup; because the mixing cup head is eccentrically connected with the mixing shaft, the mixing cup head can rotate relative to the reaction cup while rotating at a high speed, and therefore, the mixing and cleaning efficiency of the reaction cup is greatly increased.
Further, the closing mechanism comprises a jacking piece and a mouth pressing mechanism; the mouth pressing mechanism comprises a pressing block which is used for blocking the opening end of the reaction cup and rotates along with the rotation of the reaction cup; the jacking piece enables the opening end of the reaction cup to abut against the pressing block.
Further, the jacking piece is a screw motor, and a screw nut on the screw motor is fixedly mounted on the uniformly mixing substrate.
Furthermore, the mouth pressing mechanism also comprises a pressing block bearing seat, a ball bearing and a pressing block;
the ball bearing is arranged in the pressing block bearing seat;
the upper part of the pressing block is inserted in the ball bearing.
Furthermore, the upper part of the pressing block bearing seat is abutted with the spring, and the upper end of the spring is abutted with the pressing plate.
A method of using the magnetic cleaning separation device described above, comprising the steps of:
the cleaning needle assembly injects cleaning liquid into the reaction cup containing the magnetic beads;
the separation component is used for completing aggregation of the magnetic beads in the reaction cup;
the cleaning needle assembly discharges the cleaning waste liquid in the reaction cup;
the cleaning needle assembly injects cleaning liquid into the reaction cup and breaks up magnetic beads in the reaction cup;
the blending component drives the reaction cup to rotate and blocks the open end of the reaction cup when the reaction cup rotates.
The reaction cup is divided into a bowl shape formed by combining an upper cylindrical shape and a lower arc surface; the magnet is a cylindrical magnet; when magnet adsorbs the magnetic bead, the magnet minimum face is located reaction cup arc surface upper portion a bit, guarantees that magnet adsorbs the magnetic bead in the reaction cup on reaction cup arc bottom surface top lateral wall, can not take away the magnetic bead when guaranteeing the imbibition needle extraction waste liquid, avoids causing the magnetic bead loss.
The stations on the central turntable comprise a cup placing position, a liquid adding position, a magnetic adsorption station, an absorption and discharge liquid level, a mixing position, a bottom adding position, a reagent adding position and a cup taking position; the periphery of the central turntable is also provided with a circular notch which is arranged on the magnet fixing frame and used for mounting the magnet.
The magnetic bead cleaning and separating process comprises the following steps:
the method comprises the following steps that firstly, the reaction cup is grabbed to a cup placing position by the grabbers at other positions, then the central turntable is driven to rotate by the turntable motor, the reaction cup moves to two stations and reaches a liquid adding level, the cleaning needle injects cleaning liquid into the reaction cup, and the liquid adding level is set to increase the liquid level in the reaction cup to a certain degree so as to increase the adsorption capacity of a magnet and prevent uneven adsorption of magnetic beads.
Step two, magnetic adsorption step: the reaction cup moves to the magnetism that is close to magnet and adsorbs the station after the liquid feeding finishes, and magnet adsorbs the magnetic bead in with the reaction cup on the reaction cup lateral wall, and it causes the magnetic bead loss to absorb the magnetic bead when preventing follow-up absorption waste liquid, and the magnetism adsorbs the process and lasts 3 stations altogether, and every station adsorbs a plurality of seconds, guarantees the adsorption effect.
Thirdly, liquid suction and drainage step: completing the magnetic adsorption process before the reaction cup is transferred to the suction liquid level and the waste liquid is extracted, and then moving the reaction cup to the suction liquid level; the liquid suction needle arranged on the liquid suction and discharge level starts to suck the waste liquid, and a magnet is still arranged close to the liquid suction and discharge level to adsorb magnetic beads, so that the loss of the magnetic beads is prevented; after the waste liquid is absorbed, the liquid injection needle arranged at the same position starts to inject cleaning liquid, so that magnetic beads are dispersed to facilitate the mixing in the next step.
Step four, uniformly mixing: after the liquid injection action is finished, the reaction cup moves to a mixing position for first-stage mixing, and the mixing process is that a screw motor drives a mixing substrate and a mixing cup head to rise to lift the reaction cup to be in contact with the lower plane of the pressing block; after the pressure rises to the adjusted height, the reaction cup is positioned under certain pressure, then the blending motor drives the synchronizing wheel and the blending cup head to rotate, because a certain adjustable gap is formed between the blending cup head and the reaction cup, the blending cup head can drive the reaction cup to eccentrically rotate while rotating at a high speed, and meanwhile, the spring gives certain pressure to the reaction cup through the pressing block and enables the pressing block to block the opening end of the reaction cup, so that liquid in the reaction cup is prevented from splashing.
The above flow is the first-order mixing flow, then the reaction cup continues to rotate with the central turntable according to a fixed period to start the second, third and fourth-order mixing flows;
the second, third and fourth-order mixing processes all comprise a magnetic adsorption step, a liquid adsorption and drainage step, and a mixing step (the steps are the same, and the details are not repeated herein); the first, second, third and fourth-order mixing processes can be carried out synchronously, multi-order synchronous cleaning is formed, the cleaning time is saved, and the cleaning force is enhanced; after the fourth-order cleaning is finished, the reaction cup moves to the substrate adding position to add the substrate, then moves to the reagent adding position to add the reagent, and then moves to the cup taking position to be taken away by the hand grip.
The invention has the beneficial effects that:
1. the device is optimized on the existing mixing structure, the mixing efficiency is improved, and the mixture in the reaction cup is prevented from spilling out due to violent shaking of the reaction cup during mixing;
the reaction cup with the magnetic beads is placed in a bearing hole on the central turntable, and the cleaning needle assembly injects cleaning liquid into the reaction cup; the closing mechanism blocks the opening end of the reaction cup to prevent the mixture in the cup from spilling out; the mixing mechanism drives the reaction cup to rotate to mix and clean the magnetic beads; the central turntable rotates to drive the reaction cup to move to a position close to the magnetic part; the magnetic part arranged on the periphery of the central turntable adsorbs the magnetic beads to the cup wall of the reaction cup, and then the cleaning needle assembly absorbs the cleaning waste liquid to complete the primary cleaning of the magnetic beads; meanwhile, the condition that the reaction cup needs to be grabbed to carry out the mixing operation is avoided, and the mixing mode is simpler and more reliable; the cleaning effect of the magnetic beads in the reaction cup is ensured, and the testing efficiency of subsequent testing and the accuracy of the testing result are ensured.
2. The device adopts a relatively aggressive rotation mode to drive the reaction cup to rotate, so that the mixing and cleaning efficiency of the mixture in the reaction cup is obviously improved; meanwhile, the sealing mechanism is arranged to block the opening end of the reaction cup, so that liquid in the cup is prevented from spilling due to the high rotating speed of the reaction cup.
The screw motor is started to drive the screw nut to move upwards along the arrangement direction of the screw, so as to drive the blending substrate to ascend, the blending substrate ascends to drive the blending cup head to penetrate through the bearing hole to coat the lower end of the reaction cup and push the reaction cup upwards, so that the opening end of the reaction cup is jacked up and pressed by the pressing block, and the mixture in the cup is prevented from being spilled out; the blending motor drives the blending shaft to rotate through a synchronous belt transmission mechanism (a transmission structure consisting of a driving wheel, a driven wheel and a belt), and the blending shaft drives the blending cup head and the reaction cup to rotate; because the mixing cup head is eccentrically connected with the mixing shaft, the mixing cup head rotates relative to the reaction cup while rotating at a high speed, and the relative friction between the mixing cup head and the reaction cup is increased, so that the mixing and cleaning efficiency of the reaction cup is greatly increased.
3. When the pressing block for blocking the opening end of the reaction cup rotates at the lower end of the reaction cup, the pressing block blocks the upper end (the opening end of the reaction cup) of the reaction cup and rotates to a certain degree along with the rotation of the reaction cup; the pressing block gives a certain pressure to the opening end of the reaction cup under the action of the spring; the mixture in the reaction cup is prevented from overflowing, and the rotation of the reaction cup is not disturbed as much as possible.
In conclusion, the device optimizes the mixing mechanism, improves the mixing efficiency, ensures the cleaning effect of the magnetic beads in the reaction cup, and ensures the testing efficiency of the subsequent test and the accuracy of the test result.
Drawings
FIG. 1 is a schematic diagram of a magnetic cleaning and separating device for a chemiluminescent immunoassay analyzer;
FIG. 2 is a schematic view of the magnetic cleaning and separating device at another angle;
FIG. 3 is a top view of the mixing substrate;
FIG. 4 is a schematic structural view of a mouth pressing mechanism;
FIG. 5 is a cross-sectional view of a crimping mechanism;
FIG. 6 is a schematic view of the reaction cup pressed by the mouth-pressing mechanism;
FIG. 7 is a schematic view of a magnetic member adsorbing magnetic beads;
FIG. 8 is a schematic view of the structure of the cleaning needle assembly;
FIG. 9 is a schematic view of stations on the central turntable;
FIG. 10 shows a reaction cup with an elastic ring.
In the figure: 1. mounting a plate; 2. a central turntable; 3. a reaction cup; 4. a bearing hole; 5. cleaning the needle assembly; 51. a liquid suction needle; 52. injecting liquid needle; 6. a magnetic member; 7. a magnet fixing frame; 8. a turntable motor; 9. a needle frame fixing seat; 10. a guide rail mechanism; 101. a rail motor; 11. uniformly mixing the cup heads; 12. a mixing motor; 13. a driving wheel; 14. a driven wheel; 15. an idler pulley; 16. uniformly mixing the substrates; 17. a belt; 18. a blending shaft; 19. a mouth pressing mechanism; 191. pressing a block bearing seat; 192. a ball bearing; 193. briquetting; 194. a spring; 195. pressing a plate; 196. a screw; 20. a screw motor; 21. placing a cup position; 22. adding liquid level; 23. a magnetic adsorption station; 24. sucking and discharging the liquid level; 25. mixing evenly; 26. adding a bottom material level; 27. adding a reagent site; 28. taking a cup position; 29. a circular notch; 30. magnetic beads; 31. a needle frame; 32. and a frame.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
As shown in fig. 1 to 10, a magnetic cleaning and separating device for a chemiluminescent immunoassay analyzer comprises a frame 32 and a mounting plate 1 mounted on the frame 32;
the central turntable 2 is arranged on the mounting plate 1 and can rotate circumferentially relative to the mounting plate 1, and the axis of the central turntable is perpendicular to the mounting plate; the central turntable 2 is provided with a bearing hole 4 for bearing the reaction cup 3; the bearing holes are arranged along the circumferential direction of the central turntable;
the cleaning assembly is arranged on the rack 32 or the mounting plate; the cleaning assembly comprises a cleaning needle assembly 5, the cleaning needle assembly 5 is arranged above the bearing hole, and is used for injecting liquid and absorbing liquid to the reaction cup 3;
the separation assembly comprises a magnetic part 6, and the magnetic part 6 is arranged on the periphery of the central turntable to adsorb the magnetic beads 30 in the reaction cup after uniform mixing;
the blending component is arranged on the rack and comprises a sealing mechanism and a blending mechanism; the mixing mechanism drives the reaction cup to rotate; the closing mechanism blocks the opening end of the reaction cup when the reaction cup rotates.
The separating component comprises a magnet fixing frame 7 arranged around the central turntable, a magnetic part 6 is arranged in a circular notch 29 of the magnet fixing frame 7, and the magnetic part generally adopts a magnet with a customized shape; the magnet fixing frame 7 and the reaction cup turntable are concentrically arranged, and each magnet fixing slot position on the magnet fixing frame ensures that the equal-circle central angles on the circumference taking the center of the turntable as the circle center are uniformly distributed. The relative distance between each magnet and the reaction cup at the corresponding station is ensured to be equal. The magnet is close to the reaction cup as far as possible, but does not interfere the rotation of the central turntable. The magnet fixing frame is provided with a screw hole above the mounting position corresponding to the magnet, and the depth of the magnet feeding can be adjusted by utilizing a set screw, so that the distance between the magnet and the reaction cup corresponding to the station is the most appropriate distance.
The central turntable is driven by a turntable motor 8 to rotate, and the central turntable is responsible for transferring the reaction cups containing the magnetic beads 30 to corresponding operation stations; the operation stations comprise a magnetic adsorption station 23, an absorption and liquid discharge station and a blending station. The central turntable is concentrically arranged at the upper end of the central rotating shaft, the lower end of the central rotating shaft penetrates through the mounting plate and is connected with a rotating shaft of a turntable motor 8 through a transmission device (the transmission device is a synchronous belt mechanism in the embodiment, but is independent of the synchronous belt mechanism in the driving mechanism), the turntable motor 8 is arranged on the U-shaped supporting plate, and the turntable motor drives the central turntable to rotate through the transmission device and the central rotating shaft.
The cleaning needle assembly 5 comprises a liquid suction needle 51 and a liquid injection needle 52; the liquid injection needle 52 and the liquid suction needle 51 are arranged on the needle frame 31 in the form of inner and outer needles; the needle frame 31 is provided with a groove position for installing a needle fixing end block, the needle fixing end block is used for fixing the inner needle and the outer needle in a tightly holding mode, and the upper and lower positions of the inner needle and the outer needle, namely the distance from the inner needle and the outer needle to the reaction cup, can be freely adjusted; the needle frame 31 is arranged on the needle frame fixing seat 9, and the needle frame fixing seat 9 plays a role in supporting the suspended needle frame; the needle frame fixing seat 9 is connected with a sliding block on the guide rail mechanism 10, and the guide rail motor 101 drives the needle frame to move up and down through the up-and-down movement of the sliding block, so that the cleaning needle assembly can reach a proper position in the reaction cup. In this embodiment, since the cleaning needle assembly uses the inner and outer needle structure, the liquid suction needle 51 and the liquid injection needle 52 are set to the same station; therefore, a plurality of cleaning stations are reduced, and the purpose of reducing the size of the whole structure is achieved. The liquid injection needle is concentric with the liquid suction needle and wraps the liquid suction needle, and the liquid injection needle is fixedly connected with the liquid suction needle in a welding mode. When the liquid injection needle discharges cleaning liquid, waste liquid stained on the outer wall of the liquid suction needle can be cleaned at the same time, and the waste liquid in the reaction cup after cleaning is pumped away by the liquid suction needle, so that cross contamination is avoided.
In some embodiments, the liquid injection needle and the liquid suction needle can also be arranged at the same working position in a clinging manner, and the purpose of reducing the size of the whole structure can also be achieved.
Further, the blending mechanism is arranged below the bearing hole 4 and comprises a driving mechanism and a blending cup head 11; the driving mechanism drives the blending cup head 11 to rotate.
Further, the mixing cup head is in a sleeve shape; when the mixing cup head is contacted with the reaction cup, the mixing cup head covers the lower end of the reaction cup.
In some embodiments, because the lower end of the reaction cup is generally a combination of the arc surface and the aspect, the contact surface between the inner part of the blending cup head and the reaction cup is also set to be the combination of the arc surface and the aspect so as to increase the fit degree with the arc bottom surface of the reaction cup.
Further, the driving mechanism comprises a blending motor 12, the blending motor 12 is a direct current brushless motor, and a rotating shaft of the blending motor is connected with a driving wheel arranged above the blending substrate through a coupling; the driving wheel 13 is connected with a plurality of driven wheels 14 through a belt 17, and the driven wheels 14 are connected with the bottom ends of the mixing cup heads through a mixing shaft 18; the belt 17 also adjusts the degree of tension of the belt 17 through the idler pulley 15; the driving wheel 13 and the driven wheel 14 are both rotatably arranged on the blending substrate 16; the number of the driven wheels is multiple, and one driving wheel drives the driven wheels to rotate simultaneously; each driven wheel drives a mixing cup head fixedly connected with the driven wheel to drag the reaction cup to rotate; therefore, one motor can drive a plurality of reaction cups to rotate, thereby saving the space and the cost for arranging the motor.
Further, the blending cup head is eccentrically connected with the blending shaft; the inner diameter of the cross section of the mixing cup head is larger than the outer diameter of the cross section of the reaction cup; the reaction cup is inserted into the bearing hole through the elastic ring, and a gap exists between the reaction cup and the bearing hole so as to increase the shaking amplitude of the reaction cup; because the mixing cup head is eccentrically connected with the mixing shaft, the mixing cup head can rotate relative to the reaction cup while rotating at a high speed, and therefore, the mixing and cleaning efficiency of the reaction cup is greatly increased.
Further, the closing mechanism comprises a jacking piece and a mouth pressing mechanism 19; the mouth pressing mechanism 19 comprises a pressing block 193 which is used for blocking the opening end of the reaction cup and rotates along with the rotation of the reaction cup; the lift causes the open end of the reaction cup to abut the press block 193.
Further, the jacking piece is a screw motor 20, and a screw nut on the screw motor 20 is fixedly installed on the blending substrate 16.
Further, the pressure port mechanism 19 further comprises a pressure block bearing seat 191, a ball bearing 192 and a pressure block 193;
the ball bearing 192 is mounted in the press block bearing seat 191;
the upper part of the pressing block is inserted in the ball bearing 192.
Further, a spring 194 is abutted above the pressing block bearing seat 191, and the upper end of the spring 194 is abutted with a pressing plate 195; the pressing block comprises a lower pressing block and a push rod concentrically connected with the lower pressing block; the push rod is inserted into the middle of the bearing; the push rod is hollowed to form a press block threaded hole, a screw 196 is screwed into the press block threaded hole, and the head of the screw 196 presses the inner ring of the bearing; the screw connects the pressing block and the bearing together.
A method of using the magnetic cleaning separation device described above, comprising the steps of:
the washing needle assembly injects a washing liquid into the reaction cup containing the magnetic beads 30;
the separation component is used for completing aggregation of the magnetic beads in the reaction cup;
the cleaning needle assembly discharges the cleaning waste liquid in the reaction cup;
the cleaning needle assembly injects cleaning liquid into the reaction cup and breaks up magnetic beads in the reaction cup;
the blending component drives the reaction cup to rotate and blocks the open end of the reaction cup when the reaction cup rotates.
FIG. 7 shows the shape of the magnet and the reaction cup, and the comparison of the height position between the magnet and the reaction cup when the magnet adsorbs the magnetic beads in the reaction cup; the reaction cup is divided into a bowl shape formed by combining an upper cylindrical shape and a lower arc surface; the magnet in this embodiment is a cylindrical magnet; when magnet adsorbs the magnetic bead, the magnet minimum face is located reaction cup arc surface upper portion a bit, guarantees that magnet adsorbs the magnetic bead in the reaction cup on reaction cup arc bottom surface top lateral wall, can not take away the magnetic bead when guaranteeing the imbibition needle extraction waste liquid, avoids causing the magnetic bead loss.
As shown in fig. 9, the figure is a schematic diagram of stations on a central turntable, which includes a cup placing position 21, a liquid adding position 22, a magnetic adsorption position 23, an absorption and discharge liquid level 24, a blending position 25, a substrate adding position 26, a reagent adding position 27, and a cup taking position 28; the periphery of the central turntable also shows a circular notch 29, and the circular notch 29 is arranged on the magnet fixing frame and used for installing the magnet.
The magnetic bead cleaning and separating process comprises the following steps:
in the first step, the reaction cup is grabbed to the cup placing position 21 by the grabbers at other positions, then the central turntable is driven to rotate by the turntable motor, the reaction cup moves to the liquid adding position 22 after moving to two stations, the cleaning needle injects cleaning liquid into the reaction cup, and the liquid adding position 22 is set to increase the liquid level in the reaction cup to a certain degree so as to increase the adsorption capacity of the magnet and prevent uneven adsorption of magnetic beads.
Step two, magnetic adsorption step: the reaction cup moves to the magnetism adsorption station 23 that is close to magnet after the liquid feeding finishes, and magnet adsorbs the magnetic bead in the reaction cup on the reaction cup lateral wall, and it causes the magnetic bead loss to absorb the magnetic bead when preventing follow-up absorption waste liquid, and the magnetism adsorbs the process and lasts 3 stations altogether, and every station adsorbs a plurality of seconds, guarantees the adsorption effect.
Thirdly, liquid suction and drainage step: completing the magnetic adsorption process before the reaction cup is transferred to the suction and discharge liquid level 24 to extract waste liquid, and then moving the reaction cup to the suction and discharge liquid level 24; the liquid suction needle arranged on the liquid suction and discharge level starts to suck the waste liquid, and a magnet is still arranged close to the liquid suction and discharge level to adsorb magnetic beads, so that the loss of the magnetic beads is prevented; after the waste liquid is absorbed, the liquid injection needle arranged at the same position starts to inject cleaning liquid, so that magnetic beads are dispersed to facilitate the mixing in the next step.
Step four, uniformly mixing: after the liquid injection action is finished, the reaction cup moves to a mixing position 25 for first-stage mixing, and the mixing process is that a screw motor 20 drives a mixing substrate and a mixing cup head to rise to lift the reaction cup to be in contact with the lower plane of the pressing block; after the reaction cup rises to the adjusted height, the reaction cup is positioned under certain pressure, then the blending motor drives the synchronizing wheel and the blending cup head to rotate, a certain adjustable gap is formed between the blending cup head and the reaction cup, the blending cup head can drive the reaction cup to eccentrically rotate while rotating at a high speed, and meanwhile, the spring 194 gives certain pressure to the reaction cup through the pressing block and enables the pressing block to block the opening end of the reaction cup, so that liquid in the reaction cup is prevented from splashing out.
The above flow is the first-order mixing flow, then the reaction cup continues to rotate with the central turntable according to a fixed period to start the second, third and fourth-order mixing flows;
the second, third and fourth-order mixing processes all comprise a magnetic adsorption step, a liquid adsorption and drainage step, and a mixing step (the steps are the same, and the details are not repeated herein); the first, second, third and fourth-order mixing processes can be carried out synchronously, multi-order synchronous cleaning is formed, the cleaning time is saved, and the cleaning force is enhanced; after the fourth-step washing, the cuvette moves to the substrate adding position 26 to add the substrate, moves to the reagent adding position 27 to add the reagent, and then moves to the cup taking position 28 to be taken away by the hand grip.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (10)
1. A magnetic cleaning and separating device for a chemiluminescence immunoassay analyzer is characterized by comprising a frame (32) and a mounting plate (1) mounted on the frame (32);
the central turntable (2) is arranged on the mounting plate (1) and can rotate circumferentially relative to the mounting plate (1), and the axis of the central turntable is perpendicular to the mounting plate; the central turntable (2) is provided with a bearing hole (4) for bearing the reaction cup (3); the bearing holes are arranged along the circumferential direction of the central turntable;
the cleaning assembly is arranged on the rack (32) or the mounting plate; the cleaning assembly comprises a cleaning needle assembly (5), and the cleaning needle assembly (5) is arranged above the bearing hole (4) and is used for injecting liquid and absorbing liquid into the reaction cup (3);
the separation assembly comprises a magnetic part (6), and the magnetic part (6) is positioned on the periphery of the central turntable (2) to adsorb the magnetic beads (30) in the reaction cup (3) after uniform mixing;
the blending component is arranged on the rack (32) and comprises a sealing mechanism and a blending mechanism; the blending mechanism is used for driving the reaction cup (3) to rotate; when the reaction cup (3) rotates, the closing mechanism is used for blocking the opening end of the reaction cup (3).
2. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 1, wherein the mixing mechanism is arranged below the bearing hole (4), and comprises a driving mechanism and a mixing cup head (11); the driving mechanism is used for driving the blending cup head (11) to rotate.
3. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 2, wherein the mixing cup head (11) is sleeve-shaped; when the mixing cup head (11) is in contact with the reaction cup (3), the mixing cup head (11) is sleeved on the lower end of the reaction cup (3).
4. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 2, wherein the driving mechanism comprises a blending motor (12), a rotating shaft of the blending motor (12) is connected with a driving wheel (13) above a blending substrate (16) through a coupling, the driving wheel (13) is connected with a plurality of driven wheels (14) through a belt (17), and the driven wheels (14) are connected with the bottom end of the blending cup head (11) through a blending shaft (18); the driving wheel (13) and the driven wheel (14) are both rotatably arranged on the blending substrate (16).
5. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 2, wherein the mixing cup head (11) is eccentrically connected with the mixing shaft (18); the inner diameter of the cross section of the mixing cup head (11) is larger than the outer diameter of the cross section of the reaction cup (3).
6. A magnetic cleaning and separating device for a chemiluminescent immunoassay analyzer according to claim 1 wherein the closure mechanism comprises a lift and hold-down mechanism (19); the mouth pressing mechanism (19) comprises a pressing block (193) which is used for blocking the opening end of the reaction cup (3) and rotates along with the rotation of the reaction cup (3); the jacking piece enables the opening end of the reaction cup (3) to abut against the pressing block (193).
7. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 6, wherein the jacking piece is a screw motor (20), and a screw nut on the screw motor (20) is fixedly installed on the uniform mixing substrate (16).
8. The magnetic cleaning and separating device for the chemiluminescent immunoassay analyzer of claim 6 wherein the pressure port mechanism (19) comprises a pressure block bearing housing (191), a ball bearing (192) and a pressure block (193);
the ball bearing (192) is mounted in a briquetting bearing seat (191);
the upper part of the pressing block (193) is inserted into the ball bearing (192).
9. The magnetic cleaning and separating device for the chemiluminescence immunoassay analyzer according to claim 8, wherein the briquetting bearing seat (191) abuts against the spring (194) above, and the upper end of the spring (194) abuts against the pressure plate (195).
10. A method of using a magnetic cleaning separation device as claimed in any one of claims 1 to 9, comprising the steps of:
the cleaning needle assembly (5) injects cleaning liquid into the reaction cup (3) containing the magnetic beads (30);
the blending component drives the reaction cup (3) to rotate, and the opening end of the reaction cup (3) is blocked when the reaction cup (3) rotates;
the separation component enables the magnetic beads (30) in the reaction cup (3) to be completely gathered;
the cleaning needle assembly (5) drains the cleaning waste liquid in the reaction cup (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111225036.6A CN113770104A (en) | 2021-10-20 | 2021-10-20 | Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111225036.6A CN113770104A (en) | 2021-10-20 | 2021-10-20 | Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer |
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| CN113770104A true CN113770104A (en) | 2021-12-10 |
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| CN202111225036.6A Pending CN113770104A (en) | 2021-10-20 | 2021-10-20 | Magnetic cleaning and separating device and method for chemiluminescence immunoassay analyzer |
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| CN (1) | CN113770104A (en) |
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Application publication date: 20211210 |
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