CN112567250A

CN112567250A - Full-automatic magnetic bead belt cleaning device

Info

Publication number: CN112567250A
Application number: CN201980001160.9A
Authority: CN
Inventors: 张立海; 孙启鹏; 董飒英; 王吉宁; 王峰; 苏桂香
Original assignee: Yantai Addcare Bio Tech Ltd
Current assignee: Yantai Addcare Bio Tech Ltd
Priority date: 2019-07-18
Filing date: 2019-07-18
Publication date: 2021-03-26
Anticipated expiration: 2039-07-18
Also published as: CN112567250B; WO2021007853A1

Abstract

Discloses a full-automatic magnetic bead cleaning device, which comprises a bottom plate (1), a cleaning disc (13) for placing a plurality of reaction cups (12), a rotating shaft module for driving the cleaning disc (13) to rotate regularly, a magnetic bead adsorption module for adsorbing magnetic beads in the reaction cups (12), a liquid suction module for sucking liquid in the reaction cups (12), a liquid adding module for adding liquid into the reaction cups (12) and a mixing module for mixing the reaction cups (12), wherein the mixing module comprises a mixing seat (29) slidably mounted on an upright post (32), a mixing vibration motor (39), a mixing lifting motor (40), a plurality of mixing bearing units mounted on the mixing seat (29) and a plurality of mixing pressing units mounted on a fixing plate (14), the whole cleaning process can be completed by one rotation of the cleaning disc, and the cleaning efficiency is improved, the magnetic beads and the cleaning liquid are fully and uniformly mixed, the mixing effect is good, the mixing time is short, and the mixing cost is low.

Description

Full-automatic magnetic bead belt cleaning device

Technical Field

The invention relates to the technical field of magnetic bead cleaning in a full-automatic tubular chemiluminescence immunoassay analyzer, and in particular relates to a full-automatic magnetic bead cleaning device.

Background

Luminescence immunoassay is a novel ultra-microanalysis technology established by combining luminescence analysis and immunoreaction. The technology uses a chemical or biological luminous system as an indicator system of antigen-antibody reaction, thereby being a method for quantitatively detecting antigen or antibody, and the method has high sensitivity of luminous analysis and high specificity of antigen-antibody. The magnetic bead cleaning is one of the most important links in the luminescence immunoassay, and the main purpose is to wash away substances which are not combined with the solid phase antigen and the antibody in the reaction solution and interference substances which are non-specifically adsorbed on the solid phase carrier in the reaction process, and the cleaning effect directly influences the accuracy of the detection result. In the prior art, the magnetic bead cleaning device has no vibration mixing action after the cleaning solution is filled, and the cleaning solution and the magnetic beads cannot be fully mixed because the magnetic beads are adsorbed into a bulk shape, so that the magnetic bead cleaning effect is poor, and the cleaning efficiency is low; some magnetic bead cleaning devices disclose a mixing action, but generally adopt concentric rotation mixing, the mixing operation time is long, a special needle needs to be matched for adding liquid, the equipment cost is high, the mixing effect is poor, and the cleaning efficiency is low; in addition, the magnetic bead cleaning device in the prior art has a single function, the magnetic bead cleaning process is complex, actions such as magnetic attraction, liquid adding, liquid absorption, pre-excitation liquid adding and the like need to be realized on different equipment, the cleaning efficiency is low, and the equipment cost is high.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a full-automatic magnetic bead cleaning device.

The technical scheme for solving the technical problems is as follows: a full-automatic magnetic bead cleaning device comprises a bottom plate, a cleaning disc used for placing a plurality of reaction cups, a rotating shaft module used for driving the cleaning disc to rotate regularly, a magnetic bead adsorption module used for adsorbing magnetic beads in the reaction cups, a liquid suction module used for sucking liquid into the reaction cups, a liquid adding module used for adding liquid into the reaction cups and a mixing module used for mixing the reaction cups;

the rotating shaft module comprises a hollow shaft fixedly arranged on the bottom plate and a cleaning disc seat which is rotatably arranged and concentrically arranged on the hollow shaft, and the cleaning disc is concentrically and fixedly arranged on the cleaning disc seat;

the magnetic bead adsorption module comprises a magnetic suction disc, a plurality of magnets are arranged on the side face of the magnetic suction disc, a plurality of grooves used for avoiding positions of the uniformly mixing supporting head are formed in the side face of the magnetic suction disc, the bottom of the magnetic suction disc is fixedly arranged on the bottom plate through a plurality of stand columns and is positioned right below the cleaning disc, the bottom of each stand column is fixedly arranged on the bottom plate through a stand column seat, and a through hole for the cleaning disc seat to pass through is formed in the center of the magnetic suction disc;

the liquid suction module comprises a fixed plate fixedly arranged at the top of the hollow shaft, a liquid suction needle frame arranged in a lifting way relative to the fixed plate and a liquid suction unit arranged on the liquid suction needle frame and penetrating through the fixed plate;

the liquid adding module comprises a liquid adding unit arranged on the fixed plate and a liquid adding mechanism fixed on the liquid suction needle frame;

the blending module comprises a blending seat, a blending vibration motor, a blending lifting motor, a plurality of blending bearing units and a plurality of blending pressing units, the blending bearing units are arranged on the blending seat, and the blending pressing units are arranged on a fixing plate; the blending lifting motor is fixedly arranged on the bottom plate, and a blending lifting motor rod of the blending lifting motor is fixedly arranged on the blending seat; the mixing vibration motor is fixedly arranged on the bottom surface of the mixing seat, a mixing main belt wheel is arranged at the end part of a motor rod of the mixing vibration motor, the mixing main belt wheel is connected with a plurality of mixing auxiliary belt wheels through a mixing synchronous belt, and the mixing supporting unit and the mixing pressing unit are coaxial in the vertical direction;

the blending bearing unit comprises a plurality of bearing seats fixedly installed on a blending seat and blending shafts concentrically installed on the bearing seats through bearings, the blending shafts and blending slave belt wheels are coaxially and fixedly installed, blending support heads are eccentrically and rotatably installed on the upper portions of the blending shafts through the bearings, rotation preventing plates are installed on the side faces of the blending support heads, rotation preventing rods are vertically installed on the bearing seats, long holes through which the rotation preventing rods can penetrate are formed in the rotation preventing plates, top grooves are formed in the tops of the blending support heads, and the top grooves are arranged to be arc surfaces;

the mixing compresses tightly the unit and includes that the cross-section is T type and hollow mixing lid, suit spring and axle that the mixing was covered with circlip, the hollow structure of mixing lid can supply the liquid feeding needle to pass, the spring mounting is between mixing lid and fixed plate, the mixing lid passes installation axle circlip for the fixed plate back, axle circlip's external diameter is greater than the mounting hole of mixing lid on the fixed plate, mixing bottom of the lid portion sets up the bottom recess, the bottom recess sets up to the frustum that the cross-section is isosceles trapezoid.

The invention has the beneficial effects that: the rotary shaft module is arranged to drive the cleaning disc to rotate at regular time, and the magnetic bead adsorption module, the liquid absorption module and the liquid adding module are arranged, so that operations of magnetic absorption, liquid adding, uniform mixing and pre-excitation liquid adding on reaction cups on the cleaning disc are realized, and the operations are simultaneously performed on different hole sites of the cleaning disc, so that the whole cleaning process can be completed by rotating the cleaning disc for one circle, and the cleaning efficiency is improved; the magnetic beads in the reaction cup are adsorbed to the side surface of the reaction cup by arranging the magnetic chuck with the side surfaces provided with the magnets, and then the liquid absorption operation is carried out, so that the phenomenon that the magnetic beads are adsorbed to the bottom surface of the reaction cup to cause the magnetic beads to be absorbed by mistake in the liquid absorption process in the prior art can be avoided, and the influence on the accuracy of analysis data is avoided; by arranging the mixing module, the mixing bearing unit jacks up the reaction cup in situ by virtue of the mixing lifting motor, the reaction cup does not need to be taken out, liquid adding operation can be carried out simultaneously, the operation steps are compact, the cleaning time is saved, the equipment cost is saved, and the jacked reaction cup is pressed and limited by the mixing pressing unit, so that the stability of the reaction cup during mixing operation is ensured, and the phenomenon of splashing during mixing of the reaction cup is prevented; through setting up the mixing support head in the mixing bearing unit to eccentric rotation and installing on mixing axle upper portion, it is rotatory to drive the mixing from driving wheel drive mixing axle under the effect of mixing shock motor to drive the mixing support head along the eccentric rotary motion of mixing axle, thereby realize that reaction cup top compresses tightly spacingly, rotary motion's action is made to the bottom, and this kind of eccentric mode of shaking mixing carries out abundant mixing with magnetic bead and washing liquid, and the mixing is effectual, and the mixing time is short, and the mixing is with low costs.

On the basis of the technical scheme, the invention can be further improved as follows.

Further, the pivot module still includes power rotary mechanism, power rotary mechanism includes through pivot motor support mounting the pivot motor on the bottom plate, install pivot driving pulley on pivot motor pole and the pivot driven pulley of being connected through the pivot hold-in range with the pivot driving pulley, the concentric fixed mounting of pivot driven pulley and washing dish seat, the pivot motor passes through pivot motor support mounting on the bottom plate.

Adopt above-mentioned further scheme's beneficial effect is, through setting up pivot motor and pivot driving pulley and pivot driven pulley, by pivot motor drive washing dish seat synchronous revolution, and then realize the rotatory action of washing dish, provide good basis for the realization of each operation such as liquid feeding, magnetism, imbibition and mixing.

Further, wash and set up thirty six cup positions that are used for placing the reaction cup on the dish, wherein including clockwise eight of circumference range in proper order get and put the cup position, a first liquid level that adds, first four magnetism of group inhale the position, first magnetism inhale the liquid level, first liquid feeding mixing position, five magnetism of second group inhale the position, second magnetism inhale the liquid level, second liquid feeding mixing position, five magnetism of third group inhale the position, third magnetism inhale the liquid level, fourth liquid feeding mixing position, five magnetism of fourth group inhale the position, fourth magnetism inhale the liquid level and one add the pre-excitation liquid mixing position.

The beneficial effect who adopts above-mentioned further scheme is that, through setting up above-mentioned cup position, realize the repetitive operation to reaction cup liquid feeding, magnetism, imbibition, liquid feeding mixing in proper order, realize carrying out the operation that washes many times to the magnetic bead in the reaction cup, improve the cleaning performance and the cleaning efficiency of magnetic bead.

Further, the imbibition module still includes the elevator motor of installing on the fixed plate through motor support, elevator motor's elevator motor pole and imbibition needle frame fixed mounting, still set up the guide bar and the uide bushing of the relative lift slidable mounting of a plurality of between imbibition needle frame and the fixed plate, uide bushing fixed mounting is on imbibition needle frame, the one end of guide bar is fixed on the fixed plate, and the other end passes the uide bushing.

The beneficial effects of adopting above-mentioned further scheme are that, do the elevating movement through setting up elevator motor drive imbibition needle frame to make the imbibition needle descend and insert the operation that the reaction cup carries out the imbibition when realizing the imbibition, be favorable to the abluent full automatization operation of magnetic bead, improve the cleaning efficiency.

Further, the liquid suction unit comprises an equal-height screw, a spring, a buffer plate and a liquid suction needle, wherein the bottom of the equal-height screw is installed on the liquid suction needle frame in a threaded mode, the spring is sleeved on the equal-height screw, the buffer plate is installed between the spring and the liquid suction needle frame, the liquid suction needle penetrates through the liquid suction needle frame and is fixed on the buffer plate, and a hose is arranged at the top end of the liquid suction needle.

Adopt above-mentioned further scheme's beneficial effect be, through setting up equal altitude screw, spring and buffer board, when carrying out the imbibition operation to the washing liquid in the reaction cup, if the imbibition needle touches the reaction cup bottom, the spring is compressed, and the buffer board rises to prevent that the imbibition from damaging the reaction cup to the too big impact force of reaction cup bottom, be favorable to protecting the reaction cup, reduce the equipment spoilage.

Further, the liquid feeding unit is including installing the liquid feeding needle frame on the fixed plate and passing liquid feeding needle frame and carrying out the liquid feeding needle fixed, the liquid feeding needle passes the mixing lid, liquid feeding mechanism is including setting up the first liquid feeding needle on the liquid suction needle frame and exciting liquid feeding needle in advance.

The beneficial effects of adopting above-mentioned further scheme are that, carry out the liquid feeding to the reaction cup before the mixing operation through the liquid feeding unit that sets up on the fixed plate, through setting up first liquid feeding needle and the liquid feeding needle of pre-excitation liquid on the imbibition needle frame, carry out first liquid feeding and the liquid feeding operation of pre-excitation liquid to the reaction cup, set up liquid feeding unit and liquid feeding mechanism according to actual need, realize the full automatization of liquid feeding process.

Further, install the inductor frame on the bottom plate, install mixing photoelectric switch, motor monitoring photoelectric switch and zero-position sensing photoelectric switch on the inductor frame, the mixing response piece that the mixing seat goes up the installation and corresponds with mixing photoelectric switch position, install the code wheel that corresponds with motor monitoring photoelectric switch position on the washing dish seat, still install the zero-position sensing piece that corresponds with zero-position sensing photoelectric switch position on the washing dish seat.

The mixing seat has the advantages that the mixing induction sheet and the mixing photoelectric switch are arranged to monitor the operation condition of the mixing seat, so that the phenomenon that the mixing seat is blocked is prevented; the motor monitoring photoelectric switch and the coded disc are arranged to monitor the running condition of the cleaning disc seat and prevent the cleaning disc seat from being stuck; the zero induction photoelectric switch and the zero induction sheet are arranged to detect the zero position of the system.

Further, a plurality of tensioning wheels with adjustable positions are installed on the bottom surface of the blending seat, and a blending synchronous belt is installed on each tensioning wheel.

The beneficial effect who adopts above-mentioned further scheme is through setting up the take-up pulley, realizes adjusting the elasticity of mixing hold-in range, guarantees the suitable rate of tension of mixing hold-in range, prolongs the life of mixing hold-in range and improves the high efficiency of mixing operation.

Further, still set up the tensioning board on the pivot motor, tensioning board both ends fixed mounting is on pivot motor support, install the puller screw on the tensioning board, the one end of puller screw pushes up the pivot motor, and other end screw thread is installed on the tensioning board.

The beneficial effect who adopts above-mentioned further scheme is through setting up the tensioning plate, finely tunes the position of pivot motor to adjust the rate of tension of pivot hold-in range.

Furthermore, joints are arranged on the liquid adding needle, the first liquid adding needle and the pre-excitation liquid adding needle, and a joint support capable of placing a plurality of joints is arranged on the motor support.

Adopt above-mentioned further scheme's beneficial effect is, through setting up the joint, be convenient for maintain and change the liquid feeding needle, reduce the maintenance cost.

Drawings

FIG. 1 is a schematic overall perspective view of the present invention;

FIG. 2 is a schematic overall perspective view of the present invention;

FIG. 3 is a front view of the overall frame of the present invention;

FIG. 4 is a perspective view of the overall frame of the present invention;

FIG. 5 is a perspective view of a hinge module;

FIG. 6 is a perspective view of the pipetting module;

FIG. 7 is a schematic front view of the liquid suction module;

FIG. 8 is a schematic perspective view of a liquid-adding homogenizing module;

FIG. 9 is a schematic perspective view of a liquid-adding homogenizing module;

FIG. 10 is a schematic view of the mixing module;

FIG. 11 is a schematic perspective view of the blending module;

FIG. 12 is a schematic perspective view of the mixing module;

FIG. 13 is a schematic perspective view of the kneading support unit and the kneading press unit;

FIG. 14 is a schematic top view of the kneading support unit and kneading press unit;

FIG. 15 is a schematic cross-sectional view taken at A-A in FIG. 14;

FIG. 16 is an enlarged perspective view of the mixing shaft;

FIG. 17 is a schematic perspective view of the wicking unit;

FIG. 18 is a schematic top view of the wicking unit;

FIG. 19 is a schematic cross-sectional view taken at B-B in FIG. 18;

FIG. 20 is a schematic view showing the distribution of cup positions in the cleaning tray.

In the figure, 1, a bottom plate, 4, a rotating shaft motor, 5, a rotating shaft motor support, 6, a tensioning plate, 7, a rotating shaft synchronous belt, 8, a rotating shaft main belt wheel, 9, a rotating shaft secondary belt wheel, 10, a coded disc, 11, a magnetic suction disc, 12, a reaction cup, 13, a cleaning disc, 14, a fixing plate, 15, a liquid feeding needle frame, 16, a liquid suction needle frame, 17, a guide rod, 18, a guide sleeve, 19, a motor support, 20, a lifting motor, 21, a joint, 22, a joint support, 23, a guide flange, 24, a hose, 25, a liquid suction needle, 26, a liquid feeding needle, 27, a mixing cover, 28, a magnet, 29, a mixing seat, 30, a mixing synchronous belt, 31, a guide cylinder, 32, a stand column, 33, a stand column seat, 34, a support leg, 35, a mixing photoelectric switch, 36, a sensor frame, 37, a hollow shaft, 38, a cleaning disc seat, 39, a mixing vibration motor, 40, 41, a mixing lifting motor rod, 42. a lifting motor rod, 43, a zero-position sensing piece, 44, a motor monitoring photoelectric switch, 45, a zero-position sensing photoelectric switch, 46, a blending secondary belt wheel, 47, a blending sensing piece, 48, a spring, 49, a spring retainer ring for a shaft, 50, a blending support head, 51, a blending primary belt wheel, 52, an anti-rotation rod, 53, a bearing seat, 54, an anti-rotation plate, 55, a blending shaft, 56, an equal-height screw, 57, a buffer plate, 58, a first liquid adding needle, 59, a pre-excitation liquid adding needle, 60, a tension wheel, 271, a bottom groove, 501, a top groove, 551, an eccentric mounting hole, 131, a picking and placing cup position, 132, a first liquid adding position, 133, a first group of four magnetic suction positions, 134, a first magnetic suction liquid suction position, 135, a first liquid adding mixing position, 136, a second group of five magnetic suction positions, 137, a second magnetic suction position, 138, a second liquid adding position, 139, a fifth mixing position, 1310. a third magnetic suction liquid level, 1311, a fourth liquid adding and mixing level, 1312, a fourth group of five magnetic suction levels, 1313, a fourth magnetic suction liquid level, 1314, a pre-excitation liquid adding and mixing level.

Detailed Description

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.

A full-automatic magnetic bead cleaning device comprises a bottom plate 1, a cleaning disc 13, a rotating shaft module, a magnetic bead adsorption module, a liquid absorption module, a liquid adding module and a mixing module, wherein support legs 34 are arranged at the bottom of the bottom plate 1, the cleaning disc 13 is used for placing a plurality of reaction cups 12, the rotating shaft module is used for driving the cleaning disc 13 to rotate regularly, the magnetic bead adsorption module is used for adsorbing magnetic beads in the reaction cups 12, the liquid absorption module is used for absorbing liquid into the reaction cups 12, the liquid adding module is used for adding liquid into the reaction cups 12, and the mixing;

thirty-six cup positions for placing reaction cups 12 are arranged on the cleaning disc 13, wherein the cup positions comprise eight picking and placing cup positions 131, a first magnetic suction liquid level 132, a first group of four magnetic suction positions 133, a first magnetic suction liquid level 134, a first liquid adding mixing position 135, a second group of five magnetic suction positions 136, a second magnetic suction liquid level 137, a second liquid adding mixing position 138, a third group of five magnetic suction positions 139, a third magnetic suction liquid level 1310, a fourth liquid adding mixing position 1311, a fourth group of five magnetic suction positions 1312, a fourth magnetic suction liquid level 1313 and a pre-excitation liquid mixing position 1314 which are sequentially and circumferentially arranged clockwise, the picking and placing cup position 131 comprises a cup picking position and a cup placing position, the cup picking position which is arranged clockwise is positioned at the front position of the cup placing position, and the repeated operations of mixing, sucking, liquid adding and liquid adding for the reaction cups 12 are sequentially realized by arranging the cup positions, the magnetic beads in the reaction cup 12 are washed for multiple times, and the cleaning effect and the cleaning efficiency of the magnetic beads are improved.

The rotating shaft module comprises a hollow shaft 37 fixedly installed on the bottom plate 1 and a cleaning disc seat 38 which is rotatably installed and concentrically installed on the hollow shaft 37, and the cleaning disc 13 is concentrically and fixedly installed on the cleaning disc seat 38; the rotating shaft module further comprises a power rotating mechanism, the power rotating mechanism comprises a rotating shaft motor 4 arranged on the bottom plate 1 through a rotating shaft motor support 5, a rotating shaft main belt pulley 8 arranged on a rod of the rotating shaft motor 4 and a rotating shaft secondary belt pulley 9 connected with the rotating shaft main belt pulley 8 through a rotating shaft synchronous belt 7, the rotating shaft secondary belt pulley 9 and the cleaning disc seat 38 are concentrically and fixedly arranged, the rotating shaft motor 4, the rotating shaft main belt pulley 8 and the rotating shaft secondary belt pulley 9 are arranged, the cleaning disc seat 38 is driven by the rotating shaft motor 4 to synchronously rotate, further the rotating action of the cleaning disc 13 is realized, and a good foundation is provided for realizing various operations such as liquid adding, magnetic attraction, liquid absorption, uniform mixing and the; still set up tensioning plate 6 on pivot motor 4, 6 both ends fixed mounting of tensioning plate are on pivot motor support 5, install the puller screw on tensioning plate 6, the tight pivot motor 4 in one end top of puller screw, the other end screw thread is installed on tensioning plate 6, through setting up tensioning plate 6, finely tunes the position of pivot motor 4 to adjust the rate of tension of pivot hold-in range 7.

The magnetic bead adsorption module comprises a magnetic suction disc 11 with a plurality of magnets 28 mounted on the side surface, a plurality of grooves for avoiding the mixing support head 50 are formed in the side surface of the magnetic suction disc 11, the bottom of the magnetic suction disc 11 is fixedly mounted on the bottom plate 1 through a plurality of stand columns 32 and is positioned right below the cleaning disc 13, the bottom of each stand column 32 is fixedly mounted on the bottom plate 1 through a stand column seat 33, and a through hole for allowing the cleaning disc seat 38 to pass through is formed in the center of the magnetic suction disc 11;

the liquid suction module comprises a fixed plate 14 fixedly mounted at the top of a hollow shaft 37, a liquid suction needle frame 16 mounted in a lifting way relative to the fixed plate 14 and a liquid suction unit mounted on the liquid suction needle frame 16 and penetrating through the fixed plate 14; the liquid suction module further comprises a lifting motor 20 which is arranged on the fixing plate 14 through a motor bracket 19, a lifting motor rod 42 of the lifting motor 20 is fixedly arranged with the liquid suction needle frame 16 through a guide flange 23, a plurality of guide rods 17 and guide sleeves 18 which are relatively arranged in a lifting sliding mode are further arranged between the liquid suction needle frame 16 and the fixing plate 14, the guide sleeves 18 are fixedly arranged on the liquid suction needle frame 16, one end of each guide rod 17 is fixed on the fixing plate 14, the other end of each guide rod 17 penetrates through the guide sleeves 18, and the lifting motor 20 is arranged to drive the liquid suction needle frame 16 to move up and down, so that the liquid suction needle 25 is lowered to be inserted into the reaction cup 12 for liquid suction during liquid suction, full-automatic operation of magnetic bead cleaning is facilitated, and cleaning; the liquid suction unit comprises an equal-height screw 56, a spring 48, a buffer plate 57 and a liquid suction needle 25, wherein the bottom of the equal-height screw 56 is installed on the liquid suction needle frame 16 in a threaded mode, the spring 48 is sleeved on the equal-height screw 56, the buffer plate 57 is installed between the spring 48 and the liquid suction needle frame 16, the liquid suction needle 25 penetrates through the liquid suction needle frame 16, the hose 24 is arranged at the top end of the liquid suction needle 25, and through the arrangement of the equal-height screw 56, the spring 48 and the buffer plate 57, when liquid suction operation is conducted on cleaning liquid in the reaction cup 12, if the liquid suction needle 25 touches the bottom of the reaction cup 12, the spring 48 is compressed, the buffer plate 57 rises, so that the reaction cup 12 is prevented from being damaged due to overlarge impact force of the liquid suction needle 25 on the bottom of the.

The liquid adding module comprises a liquid adding unit arranged on the fixed plate 14 and a liquid adding mechanism fixed on the liquid suction needle frame 16; the liquid feeding unit is including installing liquid feeding needle frame 15 on fixed plate 14 and passing liquid feeding needle frame 15 and carrying out fixed liquid feeding needle 26, liquid feeding needle 26 passes mixing lid 27, liquid feeding mechanism is including setting up first liquid feeding needle 58 and the liquid feeding needle 59 of pre-excitation liquid on liquid suction needle frame 16, carries out the liquid feeding to reaction cup 12 before the mixing operation through setting up the liquid feeding unit of fixing on fixed plate 14, through setting up first liquid feeding needle 58 and the liquid feeding needle 59 of pre-excitation liquid on liquid suction needle frame 16, carries out first liquid feeding and the liquid feeding operation of pre-excitation liquid to reaction cup 12, sets up liquid feeding unit and liquid feeding mechanism according to actual need, realizes the full automatization of liquid feeding process.

The blending module comprises a blending seat 29, a blending vibration motor 39, a blending lifting motor 40, a plurality of blending bearing units and a plurality of blending pressing units, wherein the blending seat 29 is slidably mounted on the upright column 32 through a guide cylinder 31; the blending lifting motor 40 is fixedly arranged on the bottom plate 1, and a blending lifting motor rod 41 of the blending lifting motor 40 is fixedly arranged on the blending seat 29; the blending oscillating motor 39 is fixedly arranged on the bottom surface of the blending seat 29, a blending main belt wheel 51 is arranged at the end part of a motor rod of the blending oscillating motor 39, the blending main belt wheel 51 is connected with a plurality of blending auxiliary belt wheels 46 through a blending synchronous belt 30, and the blending supporting unit and the blending pressing unit are coaxial in the vertical direction; a plurality of tensioning wheels 60 with adjustable positions are arranged on the bottom surface of the blending seat 29, a blending synchronous belt 30 is arranged on the tensioning wheels 60, the tightness of the blending synchronous belt 30 is adjusted by arranging the tensioning wheels 60, the proper tightness of the blending synchronous belt 30 is ensured, the service life of the blending synchronous belt 30 is prolonged, and the high efficiency of blending operation is improved; the blending support unit comprises a plurality of bearing seats 53 fixedly installed on a blending seat 29 and a blending shaft 55 concentrically installed on the bearing seats 53 through bearings, the blending shaft 55 and a blending slave belt wheel 46 are coaxially and fixedly installed, a blending support head 50 is eccentrically and rotatably installed on an eccentric installation hole 551 at the upper part of the blending shaft 55 through a bearing, an anti-rotation plate 54 is installed on the side surface of the blending support head 50, an anti-rotation rod 52 is vertically installed on the bearing seat 53, a long hole through which the anti-rotation rod 52 can penetrate is formed in the anti-rotation plate 54, a top groove 501 is formed in the top of the blending support head 50, and the top groove 501 is an arc surface; the mixing compresses tightly the unit and includes that the cross-section is T type and hollow mixing lid 27, suit spring 48 and axle circlip 49 on mixing lid 27, mixing lid 27's hollow structure can supply liquid feeding needle 26 to pass, spring 48 installs between mixing lid 27 and fixed plate 14, mixing lid 27 passes installation axle circlip 49 behind the fixed plate 14, axle circlip 49's external diameter is greater than the mounting hole of mixing lid 27 on the fixed plate 14, mixing lid 27 bottom sets up bottom recess 271, bottom recess 271 sets up the frustum for the cross-section is isosceles trapezoid.

An inductor frame 36 is installed on the bottom plate 1, a blending photoelectric switch 35, a motor monitoring photoelectric switch 44 and a zero-position sensing photoelectric switch 45 are installed on the inductor frame 36, a blending induction sheet 47 corresponding to the blending photoelectric switch 35 is installed on the blending seat 29, a code disc 10 corresponding to the motor monitoring photoelectric switch 44 is installed on the cleaning disc seat 38, a zero-position induction sheet 43 corresponding to the zero-position sensing photoelectric switch 45 is also installed on the cleaning disc seat 38, and the blending induction sheet 47 and the blending photoelectric switch 35 are arranged to monitor the operation condition of the blending seat 29 and prevent the blending seat 29 from being jammed; the motor monitoring photoelectric switch 44 and the code disc 10 are arranged to monitor the running condition of the cleaning disc seat 38 and prevent the cleaning disc seat 38 from being stuck; the zero induction photoelectric switch 45 and the zero induction sheet 43 are arranged to detect the zero position of the system.

All install joint 21 on liquid adding needle 26, first liquid adding needle 58 and the excitation liquid of foreling adds liquid needle 59, the installation can place the joint support 22 of a plurality of joint 21 on the motor support 19, through setting up joint 21, is convenient for maintain and change liquid adding needle 26, reduces the maintenance cost.

Through the arrangement of the rotating shaft module for driving the cleaning disc 13 to rotate at regular time and the magnetic bead adsorption module, the liquid suction module and the liquid adding module, the operations of magnetic suction, liquid adding, uniform mixing and pre-excitation liquid adding on the reaction cup 12 on the cleaning disc 13 are realized, and the operations are simultaneously performed on different hole sites of the cleaning disc 13, so that the whole cleaning process can be completed by rotating the cleaning disc 13 for one circle, and the cleaning efficiency is improved; the magnetic beads in the reaction cup 12 are adsorbed to the side surface of the reaction cup 12 by arranging the magnetic suction disc 11 with the side surfaces provided with the magnets 28, and then the liquid suction operation is carried out, so that the phenomenon that the magnetic beads are adsorbed to the bottom surface of the reaction cup 12 in the prior art to cause the magnetic beads to be sucked by mistake during liquid suction can be avoided, and the accuracy of analysis data is prevented from being influenced; by arranging the blending module, the blending bearing unit jacks up the reaction cup 12 in situ by virtue of the blending lifting motor 40, the reaction cup 12 does not need to be taken out, liquid adding operation can be carried out simultaneously, the operation steps are compact, the cleaning time is saved, the equipment cost is saved, and the jacked reaction cup 12 is pressed and limited by the blending pressing unit, so that the stability of the reaction cup 12 in blending operation is ensured, and the phenomenon of splashing when the reaction cup 12 is blended is prevented; the mixing support head 50 in the mixing support unit is arranged on the upper portion of the mixing shaft 55 in an eccentric rotating mode, the mixing vibration motor 39 drives the mixing shaft 55 to rotate under the action of the driving mixing driven wheel 46, the mixing support head 50 is driven to do eccentric rotating motion along the mixing shaft 55, the top of the reaction cup 12 is pressed to be limited, the bottom of the reaction cup does rotating motion, magnetic beads and cleaning liquid are fully mixed in the eccentric vibrating mixing mode, the mixing effect is good, the mixing time is short, and the mixing cost is low.

The working principle is as follows: placing the reaction cup 12 at a cup placing position, rotating the rotating shaft motor 4, driving the rotating shaft driven belt wheel 9 to rotate through the rotating shaft main belt wheel 8, driving the cleaning disc 13 to rotate clockwise through the rotation of the cleaning disc seat 38 fixedly installed with the rotating shaft driven belt wheel 9, and adding liquid when the reaction cup 12 reaches the first liquid adding position 132; after the liquid is added, the cleaning disc 13 continues to rotate, the magnetic absorption operation is carried out through the first group of four magnetic absorption positions 133, then the first magnetic absorption liquid level 134 is reached, the lifting motor 20 moves to drive the liquid absorption needle frame 16 to descend, the liquid absorption needle 25 extends into the reaction cup 12, the liquid absorption operation is carried out during the magnetic absorption, the next step enters the first liquid adding mixing position 135, the mixing lifting motor 40 moves to enable the mixing seat 29 to ascend, so that the mixing support head 50 ascends to support the reaction cup 12, the liquid absorption needle 26 injects cleaning liquid into the reaction cup, the mixing oscillating motor 39 moves, the mixing main belt wheel 51 and the mixing auxiliary belt wheel 46 drive the mixing shaft 55 to rotate, so that the mixing support head 50 is driven to carry out eccentric rotation movement, so that the eccentric oscillation movement of the reaction cup 12 is completed, the next step enters the second group of five magnetic absorption positions 136 to carry out the magnetic absorption operation, then the second magnetic absorption liquid level 137 is reached, and (3) rotating the reaction cup sequentially to pass through a second liquid adding and mixing position 138, a third group of five magnetic suction positions 139, a third magnetic suction liquid level 1310, a fourth liquid adding and mixing position 1311, a fourth group of five magnetic suction positions 1312, a fourth magnetic suction liquid level 1313 and a pre-excitation liquid mixing position 1314 to perform corresponding operations, and finally, the reaction cup 12 reaches a cup taking position, so that the cleaning process is completed. When the cleaning disc rotates one hole site, all the cup sites are moved simultaneously, so that the operations of continuously placing the cup, adding liquid, magnetically attracting, absorbing liquid, uniformly mixing the added liquid, taking the cup and the like are realized.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, but rather as the subject matter of the invention is to be construed as broadly as the appended claims.

Claims

The utility model provides a full-automatic magnetic bead belt cleaning device which characterized in that: the device comprises a bottom plate, a cleaning disc for placing a plurality of reaction cups, a rotating shaft module for driving the cleaning disc to rotate at regular time, a magnetic bead adsorption module for adsorbing magnetic beads in the reaction cups, a liquid absorption module for absorbing liquid into the reaction cups, a liquid adding module for adding liquid into the reaction cups and a mixing module for mixing the reaction cups; the rotating shaft module comprises a hollow shaft fixedly arranged on the bottom plate and a cleaning disc seat which is rotatably arranged and concentrically arranged on the hollow shaft, and the cleaning disc is concentrically and fixedly arranged on the cleaning disc seat;

the magnetic bead adsorption module comprises a magnetic suction disc with a plurality of magnets mounted on the side surface, the bottom of the magnetic suction disc is fixedly mounted on the bottom plate through a plurality of stand columns and is positioned right below the cleaning disc, and a through hole for the cleaning disc seat to pass through is formed in the center of the magnetic suction disc;

the liquid suction module comprises a fixed plate fixedly arranged at the top of the hollow shaft, a liquid suction needle frame arranged in a lifting way relative to the fixed plate and a liquid suction unit arranged on the liquid suction needle frame and penetrating through the fixed plate;

the liquid adding module comprises a liquid adding unit arranged on the fixed plate and a liquid adding mechanism fixed on the liquid suction needle frame;

the mixing module comprises a mixing seat, a mixing vibration motor, a mixing lifting motor, a plurality of mixing bearing units and a plurality of mixing compression units, wherein the mixing seat is slidably mounted on an upright post;

the blending bearing unit comprises a plurality of bearing seats fixedly installed on a blending seat and blending shafts concentrically installed on the bearing seats through bearings, the blending shafts and blending slave belt wheels are coaxially and fixedly installed, blending support heads are eccentrically and rotatably installed on the upper portions of the blending shafts through the bearings, rotation preventing plates are installed on the side faces of the blending support heads, rotation preventing rods are vertically installed on the bearing seats, long holes through which the rotation preventing rods can penetrate are formed in the rotation preventing plates, top grooves are formed in the tops of the blending support heads, and the top grooves are arranged to be arc surfaces;

the mixing compresses tightly the unit and includes that the cross-section is T type and hollow mixing lid, suit spring and axle that the mixing was covered with circlip, the hollow structure of mixing lid can supply the liquid feeding needle to pass, the spring mounting is between mixing lid and fixed plate, the mixing lid passes installation axle circlip for the fixed plate back, axle circlip's external diameter is greater than the mounting hole of mixing lid on the fixed plate, mixing bottom of the lid portion sets up the bottom recess, the bottom recess sets up to the frustum that the cross-section is isosceles trapezoid.
The automatic magnetic bead washing device of claim 1, wherein: the rotating shaft module further comprises a power rotating mechanism, the power rotating mechanism comprises a rotating shaft motor arranged on the bottom plate through a rotating shaft motor support, a rotating shaft main belt wheel arranged on a rotating shaft motor rod and a rotating shaft driven belt wheel connected with the rotating shaft main belt wheel through a rotating shaft synchronous belt, and the rotating shaft driven belt wheel and the cleaning disc seat are fixedly arranged in a concentric mode.
The apparatus of claim 1 or 2, wherein: wash and set up thirty six cup positions that are used for placing the reaction cup on the dish, wherein including clockwise eight of circumference range in proper order get put the cup position, add the liquid level for the first time, first four magnetism of group inhale the position, first magnetism is inhaled the liquid level, first liquid feeding mixing position, five magnetism of second group inhale the position, second magnetism is inhaled the liquid level, second liquid feeding mixing position, five magnetism of third group inhale the position, third magnetism is inhaled the liquid level, fourth liquid feeding mixing position, five magnetism of fourth group inhale the position, fourth magnetism is inhaled the liquid level and one and is added the mixing position of pre-excitation liquid.
The apparatus of claim 1 or 2, wherein: the imbibition module is still including installing the elevator motor on the fixed plate through motor support, elevator motor's elevator motor pole and imbibition needle frame fixed mounting, still set up the guide bar and the uide bushing of the relative lift slidable mounting of a plurality of between imbibition needle frame and the fixed plate, uide bushing fixed mounting is on imbibition needle frame, the one end of guide bar is fixed on the fixed plate, and the other end passes the uide bushing.
The apparatus of claim 4, wherein: the liquid suction unit comprises an equal-height screw, a spring, a buffer plate and a liquid suction needle, wherein the bottom of the equal-height screw is installed on the liquid suction needle frame in a threaded mode, the spring is sleeved on the equal-height screw, the buffer plate is installed between the spring and the liquid suction needle frame, the liquid suction needle penetrates through the buffer plate and is fixed on the buffer plate, and the liquid suction needle penetrates through the liquid suction needle frame.
The apparatus of claim 1 or 2, wherein: the liquid feeding unit is including installing the liquid feeding needle frame on the fixed plate and passing liquid feeding needle frame and carrying out the liquid feeding needle that fixes, the liquid feeding needle passes the mixing lid, liquid feeding mechanism is including setting up the first liquid feeding needle on the liquid suction needle frame and exciting liquid feeding needle in advance.
The automatic magnetic bead washing device of claim 1, wherein: the cleaning device is characterized in that an inductor frame is installed on the bottom plate, a blending photoelectric switch, a motor monitoring photoelectric switch and a zero-position sensing photoelectric switch are installed on the inductor frame, a blending induction sheet corresponding to the blending photoelectric switch is installed on a blending seat, a coded disc corresponding to the motor monitoring photoelectric switch is installed on a cleaning disc seat, and a zero-position induction sheet corresponding to the zero-position sensing photoelectric switch is installed on the cleaning disc seat.
The automatic magnetic bead washing device of claim 1, wherein: the bottom surface of the blending seat is provided with a plurality of tensioning wheels with adjustable positions, and the tensioning wheels are provided with blending synchronous belts.
The apparatus of claim 2, wherein: the rotating shaft motor is further provided with a tensioning plate, two ends of the tensioning plate are fixedly mounted on a rotating shaft motor support, a jacking screw is mounted on the tensioning plate, one end of the jacking screw jacks the rotating shaft motor, and the other end of the jacking screw is mounted on the tensioning plate in a threaded mode.
The apparatus of claim 6, wherein: joints are arranged on the liquid adding needle, the first liquid adding needle and the pre-excitation liquid adding needle, and joint supports capable of placing a plurality of joints are arranged on the motor supports.