WO2019000706A1

WO2019000706A1 - Method for cleaning magnetic beads of analyzer

Info

Publication number: WO2019000706A1
Application number: PCT/CN2017/106253
Authority: WO
Inventors: 郭金龙; 沙利烽; 吴冬
Original assignee: 苏州长光华医生物医学工程有限公司
Priority date: 2017-06-27
Filing date: 2017-10-16
Publication date: 2019-01-03
Also published as: CN107262444A; CN107262444B

Abstract

A method for cleaning the magnetic beads of an analyzer, comprising the following steps: S1: placing a reaction cup (900) into a cleaning tray (2); S2: rotating the cleaning tray (2) such that the reaction cup (900) sequentially passes through magnetic pole pairs (81, 82), the magnetic pole directions of magnets in each magnetic pole pair (81, 82) being the same, and the magnetic pole directions of two adjacent magnetic pole pairs (81, 82) being opposite; S3: injecting a liquid after liquid suction; S4: shaking well; S5: repeating steps S2-S4 three times or more; S6: continuously rotating the cleaning tray (2) such that the reaction cup (900) passes through magnetic pole pairs (81, 82) in which heights gradually decrease; S7: suctioning the liquid within the reaction cup (900). By means of the present method, magnetic beads within the reaction cup (900) are attracted by the means of magnetic pole pairs (81, 82) such that the magnetic beads are located on front and rear walls of the reaction cup (900), and thus when a liquid suction and injection needle that is inserted into the reaction cup (900) suctions a liquid, the magnetic beads within the reaction cup (900) will not be suctioned, while a change in the N-S poles of each magnetic pole pair (81, 82) causes the magnetic beads within the reaction cup (900) to move during an interval between liquid suction such that the magnetic beads sufficiently contact the cleaning liquid so as to wash off reagents which adhere to the surfaces of the magnetic beads.

Description

Title of the invention: an analyzer magnetic bead cleaning method

Technical field

[0001] The present invention relates to an analyzer magnetic bead cleaning method, and belongs to the technical field of luminescence immunoassay.

Background technique

[0002] Chemiluminescence immunoassay (CLIA) combines highly sensitive chemiluminescence assays with highly specific immunoassays for various antigens, haptens, antibodies, hormones, enzymes, fatty acids. , detection and analysis techniques for vitamins and drugs. It is usually necessary to coat the specific biological material on the magnetic beads, and then add the luminescent liquid to collect the luminescence intensity to quantitatively analyze the detected substance.

[0003] The analyzer disc cleaning device is manufactured for continuous measurement, and the reaction cup is washed a plurality of times on a rotating disc to wash the specific biological material not coated on the magnetic beads. Some analyzer disc cleaning devices do not have a good cleaning effect, and it is easy to take the magnetic beads together with the cleaning liquid, thereby affecting the accuracy of the measurement.

technical problem

The technical problem to be solved by the present invention is: To overcome the above problems, an analyzer magnetic bead cleaning method with better cleaning effect is provided.

[0005]

Problem solution

Technical solution

[0006] The present invention provides an analyzer magnetic bead cleaning method, comprising the following steps:

[0007] S1: placing the cuvette containing the magnetic beads into the rotating cleaning tray;

[0008] S2: rotating the cleaning disk so that the reaction cup sequentially passes through the pair of magnetic poles composed of at least two pairs of magnets, the magnetic poles of the magnets in each magnetic pole pair have the same direction, and the magnetic poles of the adjacent two magnetic pole pairs are opposite in direction;

[0009] S3: sucking and refilling the liquid in the reaction cup at the position of the last pair of magnetic poles;

[0010] S4: shaking the reaction cup;

[0011] S5: the cleaning disk continues to rotate, repeating the S2-S4 step three times or more; [0012] S6: continuing to rotate the cleaning disk so that the cuvettes of the at least two pairs of magnets whose cuvettes are gradually lowered from the height are sequentially passed through;

[0013] S7: The liquid in the reaction cup is sucked away.

[0014] Preferably, in the analyzer magnetic bead cleaning method of the present invention, in the step S4, the cuvette is horizontally oscillated and shaken.

[0015] Preferably, in the analyzer magnetic bead cleaning method of the present invention, in the step S6, the latter magnetic pole pair is lowered by 5-8 mm compared with the previous magnetic pole pair.

[0016] Preferably, in the analyzer magnetic bead cleaning method of the present invention, the reaction cup is also under a vertical magnetic field during the shaking process before the S6 step.

[0017] Preferably, the analyzer magnetic bead cleaning method of the present invention rotates the cuvette at a speed of 800-1200 rpm for 8-15 s.

[0018] Preferably, the analyzer magnetic bead cleaning method of the present invention holds the reaction cup for 5-15 s in the middle of each pair of magnetic poles.

[0019] Preferably, the analyzer magnetic bead cleaning method of the present invention is a neodymium boron ferrite magnet.

[0020] Preferably, the analyzer magnetic bead cleaning method of the present invention sequentially passes through 6 magnetic pole groups, 5 times shaking, 5 times aspirating, 4 times injecting liquid.

[0021] Preferably, in the analyzer magnetic bead cleaning method of the present invention, each magnetic pole group has two magnetic pole pairs, a total of 12 magnetic pole pairs, and the magnetic pole pairs of the magnetic pole pairs are sequentially NS, NS, SN, SN; NS, NS, SN, S-N; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN.

[0022] The beneficial effects of the present invention are: the analyzer magnetic bead cleaning method of the present invention, the magnetic beads in the reaction cup are attracted by the magnetic pole pairs located on both sides of the reaction cups of the plurality of reaction cups, so that the magnetic beads are located on the front and rear walls of the reaction cup. Therefore, the liquid absorbing liquid in the reaction cup is inserted into the reaction cup, and the magnetic beads in the reaction cup are not sucked. At the same time, the magnetic beads in the reaction cup in the gap of the liquid absorption are moved by the change of the N-S poles of the respective magnetic poles, and the magnetic beads are further brought into contact with the cleaning liquid to wash off the reagent which is not adhered to the surface of the magnetic beads.

Advantageous effects of the invention

Brief description of the drawing

DRAWINGS

[0023] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. 1 is a perspective structural view of an embodiment of an analyzer reagent cleaning device of the present invention;

2 is a schematic view showing a magnetic pole arrangement on a disk of the present invention;

3 is a perspective structural view of an embodiment of a cleaning disk mechanism of the present invention;

4 is a perspective structural view of an embodiment of a shaker mechanism of the present invention.

[0028] The reference numerals are:

[0029] 1-cylinder; 11-reaction cup running tank; 2-disc; 3-shake mechanism; 310-shake drive member; 311-rocker; 321-elastic sheet; 322-cover wheel; Eccentric cup; 400-loading mechanism; 410-electromagnetic drive; 420-rack; 430-swing plate; 500-feeding mechanism; 900-reaction cup.

Detailed ways

The invention will now be described in further detail with reference to the drawings. The drawings are simplified schematic diagrams, and only the basic structure of the invention is illustrated in a schematic manner, and thus only the configurations related to the present invention are shown.

Embodiment 1

[0032] This embodiment provides an analyzer disc cleaning device, as shown in FIG. 1:

[0033] washing disk mechanism, liquid suction injection mechanism and shaking mechanism 3;

[0034] The cleaning disk mechanism includes:

[0035] The cylinder 1 has a circular reaction cup running tank 11;

[0036] The disc 2 is rotatably disposed in the cylinder 1, and the disc 2 is equidistantly arranged with a circle of reaction cup accommodating holes, and the reaction cup 900 can be placed in the reaction cup accommodating hole;

[0037] A plurality of magnetic pole groups are disposed in the cylinder 1 in sequence along the running direction of the cuvette 900, each magnetic pole group having a pair of magnetic poles located on opposite sides of the plurality of cuvette running grooves 11, each magnetic pole group having at least two The pair of magnetic poles located on both sides of the cuvette running groove 11 has the same magnetic pole direction of each magnet pair in each magnetic pole group, and the magnetic poles of the adjacent two magnetic pole pairs are opposite in direction, and the magnetic pole pairs of the last magnetic pole group are gradually set in height. Lowering, the height of the latter pole is 5-8 mm lower than that of the previous pole pair;

[0038] The shaking mechanism 3 is located between the two magnetic pole sets, and includes: a lifting and lowering driving member, a lifting plate, a rocking driving member 310 fixed at the bottom of the cylinder 1 10, and connected to the shaking driving member 310 through the coupling The rocker 311, the eccentric cup 330 disposed on the top of the rocker 311, the elastic piece 321 fixed on the cylinder 110 and extending into the top of the disc 120, and the elastic piece 321 is provided with a cover for covering the top of the reaction cup 900 Wheel 322, the bottom of the cover wheel 322 has energy The circular wheel extends into the reaction cup 900. The central axis of the eccentric cup 330 is not collinear with the central axis of the rocker 311, and the distance between the central axis of the eccentric cup 330 and the central axis of the rocker 311 is 1 of the width of the reaction cup 900. /4-1/5, shaking the reaction cup 900, the lifting drive member drives the lifting plate to rise, the shaking driving member 310, the rocking rod 311, the eccentric cup 330 are raised with the lifting plate, and the eccentric cup 330 will be the reaction cup 900 is lifted up, the top of the reaction cup 900 is pressed against the cover wheel 322 of the elastic piece 321, the reaction cup is fixed 900 times, then the drive member 310 is started, and the cuvette 900 is shaken by the eccentric cup 330 to make the liquid in the reaction cup 900 Shake well, the bottom of the cover wheel 322 has a layer of silicone rubber, shake it evenly, so that the reaction cup rotates at a speed of 800-1200 rpm for 8-15 s;

[0039] All magnets are neodymium boron ferrite magnets.

[0040] The eccentric cup 330 of the shaker mechanism 3 in front of the last magnetic pole group has magnetic properties, and the magnetic poles are located at both ends of the top and bottom.

[0041] The liquid-absorbent liquid injection needle 4 of the liquid-absorbent liquid-injecting mechanism can absorb and inject liquid in the reaction cup 900 at a position corresponding to the last magnetic pole pair of each magnetic pole group, which requires aspiration and liquid injection. After the end, the liquid infusion needle 4 is taken out, and except for the last liquid infusion needle 4, the other liquid infusion needles 4 have the function of aspirating and injecting liquid, and the last aspirating liquid is injected. The liquid needle 4 has only a function of aspirating.

[0042] The analyzer disc cleaning device of the embodiment attracts the magnetic beads in the reaction cup 900 through the pair of magnetic poles located on both sides of the plurality of cuvette operation slots 11, so that the magnetic beads are located on the front and rear walls of the reaction cup 900, thereby Insertion into the cuvette 900 to aspirate the infusion needle 4 to aspirate the magnetic beads in the cuvette 900. At the same time, by changing the N-S pole of each magnetic pole, the magnetic beads in the reaction cup 900 are moved in the gap of the liquid absorption, and the magnetic beads are further brought into contact with the cleaning liquid to wash off the reagent which is not adhered to the surface of the magnetic beads.

Embodiment 2

[0044] This embodiment provides an analyzer magnetic bead cleaning method, using the apparatus of the embodiment 1 to achieve the following steps: [0045] S1: placing the reaction cup containing the magnetic beads into the rotating cleaning disk;

[0046] S2: rotating the cleaning disk so that the reaction cup sequentially passes through the pair of magnetic poles composed of at least two pairs of magnets, the magnetic poles of the magnets in each pair of magnetic poles have the same direction, and the magnetic poles of the adjacent two magnetic pole pairs are opposite in direction;

[0047] S3: sucking and refilling the liquid in the cuvette at the position of the last pair of magnetic poles;

[0048] S4: shaking the reaction cup, and swinging the reaction cup horizontally;

[0049] S5: the cleaning disk continues to rotate, repeating the S2-S4 step three times or more;

[0050] S6: continuing to rotate the cleaning disk so that the cuvettes of the at least two pairs of magnets whose height gradually decreases from the height are sequentially passed, and the height of the latter pair of poles is reduced by 5-8 mm compared with the previous pair of poles; [0051] S7: The liquid in the reaction cup is sucked away.

[0052] Further, during the shaking process before the step S6, the reaction cup is still under the action of a vertical magnetic field.

[0053] Further, in addition to the last shake, the reaction cup is rotated at a speed of 800-1200 rpm for 8-15 s.

[0054] Further, the cuvette stays in the middle of each pair of poles for 5-15 s.

[0055] Further, the reaction cup passes through 6 magnetic pole groups in sequence, 5 times shaking, 5 times aspiration, and 4 injections.

[0056] Further, each magnetic pole group has two magnetic pole pairs, a total of 12 magnetic pole pairs, the magnetic pole pairs of the magnetic pole pairs are NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN.

[0057] The analyzer magnetic bead cleaning method of the embodiment attracts the magnetic beads in the reaction cup 900 through the pair of magnetic poles located on both sides of the plurality of cuvette running grooves 11, so that the magnetic beads are located on the front and rear walls of the reaction cup 900, thereby Insertion into the cuvette 900 to aspirate the infusion needle 4 to aspirate the magnetic beads in the cuvette 900. At the same time, by changing the N-S pole of each magnetic pole, the magnetic beads in the reaction cup 900 are moved in the gap of the liquid absorption, and the magnetic beads are further brought into contact with the cleaning liquid to wash off the reagent which is not adhered to the surface of the magnetic beads.

[0058] The above-described embodiments of the present invention are intended to be able to make various changes and modifications within the scope of the technical scope of the present invention. The technical scope of the present invention is not limited to the contents of the specification, and the technical scope thereof must be determined in accordance with the scope of the claims.

Claims

Claim

[Claim 1] An analyzer magnetic bead cleaning method, comprising the steps of:

S1: placing the cuvette containing the magnetic beads into the rotating cleaning disk; S2: rotating the cleaning disk so that the cuvette passes through the pair of magnetic poles composed of at least two pairs of magnets in sequence, and the magnetic poles of each magnetic pole pair have the same magnetic pole direction The magnetic poles of two adjacent magnetic pole pairs are opposite in direction;

S3: sucking and refilling the liquid in the reaction cup at the position of the last magnetic pole pair; S4: shaking the reaction cup;

S5: The cleaning disk continues to rotate, and the S2-S4 step is repeated 3 times or more;

S6: continuing to rotate the cleaning disk so that the cuvette passes through the pair of magnetic poles formed by at least two pairs of magnets whose height gradually decreases;

S7: The liquid in the cuvette is sucked away.

[Claim 2] The analyzer magnetic bead cleaning method according to claim 1, wherein in the step S4, the cuvette is horizontally oscillated and shaken.

[Claim 3] The analyzer magnetic bead cleaning method according to claim 1, wherein in the step S6, the latter magnetic pole pair is lowered in height by 5-8 mm as compared with the previous magnetic pole pair.

[Claim 4] The analyzer magnetic bead cleaning method according to claim 1, wherein the reaction cup is still under a vertical magnetic field during the shaking before the step S6.

[Claim 5] The analyzer magnetic bead cleaning method according to claim 1, wherein the cuvette is rotated at a speed of 800 to 1200 rpm for 8-15 s.

[Claim 6] The analyzer magnetic bead cleaning method according to claim 1, wherein the cuvette stays in the middle of each pair of magnetic poles for 5-15 s.

[Aspect 7] The analyzer magnetic bead cleaning method according to any one of claims 1 to 6, wherein the magnet is a neodymium boron ferrite magnet.

[Claim 8] The analyzer magnetic bead cleaning method according to claim 7, wherein the reaction cup passes through 6 magnetic pole groups, 5 times shaking, 5 times aspirating, and 4 times injecting.

[Claim 9] The analyzer magnetic bead cleaning method according to claim 8, wherein each of the magnetic pole groups has two magnetic pole pairs, a total of 12 magnetic pole pairs, and the magnetic pole pairs of the magnetic pole pairs are sequentially NS. NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, SN; NS, NS, SN, S-

N.