CN108562740B - ELISA plate liquid level detection device and detector - Google Patents
ELISA plate liquid level detection device and detector Download PDFInfo
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- CN108562740B CN108562740B CN201810711502.3A CN201810711502A CN108562740B CN 108562740 B CN108562740 B CN 108562740B CN 201810711502 A CN201810711502 A CN 201810711502A CN 108562740 B CN108562740 B CN 108562740B
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- 239000007788 liquid Substances 0.000 title claims abstract description 78
- 238000002965 ELISA Methods 0.000 title claims abstract description 72
- 238000001514 detection method Methods 0.000 title claims abstract description 41
- 238000006243 chemical reaction Methods 0.000 claims abstract description 63
- 230000004308 accommodation Effects 0.000 claims abstract description 13
- 238000005192 partition Methods 0.000 claims abstract description 7
- 239000012780 transparent material Substances 0.000 claims description 5
- 239000003086 colorant Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 description 5
- 239000012916 chromogenic reagent Substances 0.000 description 4
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
- G01F23/22—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water
- G01F23/28—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm by measuring physical variables, other than linear dimensions, pressure or weight, dependent on the level to be measured, e.g. by difference of heat transfer of steam or water by measuring the variations of parameters of electromagnetic or acoustic waves applied directly to the liquid or fluent solid material
- G01F23/284—Electromagnetic waves
- G01F23/292—Light, e.g. infrared or ultraviolet
- G01F23/2921—Light, e.g. infrared or ultraviolet for discrete levels
- G01F23/2928—Light, e.g. infrared or ultraviolet for discrete levels using light reflected on the material surface
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Abstract
The invention provides an ELISA plate liquid level detection device and a detector, which relate to the technical field of experimental equipment and comprise a placement part and a light emitter; the placing part is provided with a plurality of containing units which respectively correspond to the micro reaction tanks of the ELISA plates; the light emitter includes: the light source comprises a containing groove, a top plate, a partition plate, a light source and a control switch; the opening end of the accommodating groove is connected with the top plate; the plurality of clapboards are used for dividing the accommodating cavity into a plurality of strip-shaped darkroom units; the top plate is provided with a plurality of light outlet holes, and the light outlet holes at the top end of each darkroom unit are arranged along the direction parallel to the darkroom unit; the placing part is arranged at one side of the top plate far away from the accommodating groove; the accommodation unit is provided with the light trap for in projecting the light of light source emission to setting up the micro-reaction tank in the accommodation unit through light-emitting hole and light trap, with the liquid level condition in every micro-reaction tank of observation. The ELISA plate liquid level detection device provided by the invention is convenient in detecting the liquid level of the solution and easy to popularize.
Description
Technical Field
The invention relates to the technical field of experimental equipment, in particular to an ELISA plate liquid level detection device and a detector.
Background
In the process of developing an enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA) in a laboratory, the ELISA plate plays an important role. The ELISA plate is made of polystyrene, and antigen or antibody is coated on the wall of a micro-reaction tank of the ELISA plate at a certain concentration during the action, and the ELISA plate is used for detecting target substances in serum, cell culture fluid, body fluid and the like by adopting various methods such as indirect and competition, and the like through color development and OD value measurement by an ELISA plate, so that the ELISA plate can be qualitative or quantitative and is the most commonly used immunological detection means at present. The ELISA plate can be divided into 48 holes and 96 holes according to the number of the holes. 96 wells are commonly used. Is widely used in various universities and scientific institutions and bioengineering companies.
The 96-well ELISA plate comprises 96 micro-reaction cells, and the 96 micro-reaction cells are arranged in a matrix of 12 x 8. The 96 ELISA plate comprises a fixed ELISA plate and a detachable ELISA plate. The plurality of micro reaction tanks of the detachable ELISA plate are uniformly arranged on the support plate and are detachably connected with the support plate.
The reaction solution in the 96-well ELISA plate is mostly transparent. After the micro-reaction cells in the 96-well ELISA plate are filled with liquid, it is necessary to observe whether the liquid amounts in the micro-reaction cells in the same row or column are consistent. In the prior art, a color reagent is usually added into a sample diluent to judge whether the liquid adding amount of the reaction liquid in the micro-reaction tank is consistent or whether the liquid leakage condition exists (the liquid leakage phenomenon may occur in the liquid adding process in some micro-reaction tanks).
At present, some laboratories at home and abroad adopt methods of adding color reagent into coating liquid, sample diluent and secondary antibody diluent to judge whether the liquid adding amount in a micro reaction tank is consistent or not and whether a leak hole phenomenon exists or not, but the formulas of the color reagent in each laboratory are kept secret for protecting the intellectual property rights of the laboratory, so that the popularization of a detection test is not facilitated.
Disclosure of Invention
The invention aims to provide an ELISA plate liquid level detection device and a detector, which are used for solving the technical problem that the liquid level height and the leakage hole condition in a micro hole of an ELISA plate are not easy to judge in the prior art.
The invention provides an ELISA plate liquid level detection device, which comprises a placement part and a light emitter;
the placing part is provided with a plurality of accommodating units, and the plurality of accommodating units respectively correspond to the micro-reaction tanks of the ELISA plate so that the accommodating units can correspondingly accommodate the plurality of micro-reaction tanks of the ELISA plate;
the light emitter includes: the light source comprises a containing groove, a top plate, a partition plate, a light source and a control switch; the opening end of the accommodating groove is connected with the top plate to form an accommodating cavity; the number of the partition plates and the number of the light sources are multiple; the plurality of baffles are arranged in the accommodating cavity along a preset direction and are used for dividing the accommodating cavity into a plurality of strip-shaped darkroom units; the light sources are respectively arranged in the darkroom unit, and the light sources are arranged along the extending direction of the darkroom unit; the top plate is provided with a plurality of light emitting holes, the light emitting holes respectively correspond to the darkroom units, and the light emitting holes at the top end of each darkroom unit are arranged along the direction parallel to the darkroom units; the placing part is arranged at one side of the top plate far away from the accommodating groove; the light emitting holes are corresponding to the light transmitting holes of the accommodating unit and are used for projecting light emitted by the light source into the micro reaction tank arranged in the accommodating unit through the light emitting holes and the light transmitting holes; so as to observe the liquid level condition in each micro-reaction tank;
the control switch is electrically connected with the light sources and is used for correspondingly controlling the opening and closing of the light sources.
Further, the inner wall of the accommodating unit is provided with an opaque shading layer; the light source is configured as a monochromatic light source.
Further, the colors of the light sources in adjacent darkroom units are different.
Further, a plurality of the accommodation units are arranged in the first direction and the second direction; the first direction and the second direction are perpendicular; the number of the accommodating units arranged in the first direction is greater than the number of the accommodating units in the second direction.
Further, a plurality of the accommodation units are arranged in the first direction and the second direction; the first direction and the second direction are perpendicular; the number of the containing units arranged in the first direction and the second direction is the same, and the number of the containing units arranged in the first direction and the second direction is the same as the maximum number of the micro reaction tanks in the first direction and the second direction of the ELISA plate.
Further, the number of the accommodation units in the first direction and the second direction of the placement portion is set to 12.
Further, the light emitter includes a limiting portion connected to the placement portion, so that the light emitting hole corresponds to the light transmitting hole of the accommodating unit.
Further, the top plate and the receiving groove are detachably connected.
Further, the edge of the placement portion is provided with a recess for forming a gap between the microplate and the recess.
The invention also provides a detector, which comprises a cover plate and the ELISA plate liquid level detection device;
the cover plate is made of transparent materials, and is provided with a plurality of liquid adding holes, and the liquid adding holes respectively correspond to the containing units and are used for sucking or sucking out culture solution through the cover plate.
When the ELISA plate liquid level detection device provided by the invention is used for detecting, the ELISA plate is placed in the accommodating unit, the light source forms a reflecting surface on the liquid level in the micro-reaction tank through the light outlet hole and the light transmitting hole, and the liquid level condition in the micro-reaction tank can be conveniently observed through the reflecting surface. If the same amount of liquid is counted in the micro-reaction cell, the light reflection surface in the micro-reaction cell should be uniform, and the reflection surface is on the same horizontal plane when viewed in a slightly inclined direction from the top. If the micro-reaction tanks have no reflecting surface or are lower than the average reflecting surface, the leakage holes or the sample adding amount are judged to be inconsistent, so that the liquid adding amount in each micro-reaction tank can be consistent through a liquid supplementing mode. The liquid level condition of the corresponding micro reaction tanks in the columns (or rows) can be observed through the turned-on light source when the liquid level detector is turned on, and the liquid is timely replenished, so that the detection is more convenient, and the liquid level condition judgment is more accurate. Compared with the detection by a chromogenic reagent in the prior art, the ELISA plate liquid level detection device provided by the invention has the advantages that the detection of the liquid level condition of the solution is more convenient, and the popularization is easy.
The detector provided by the invention comprises a cover plate and the ELISA plate liquid level detection device; the cover plate is made of transparent materials, and is provided with a plurality of liquid adding holes, and the liquid adding holes respectively correspond to the containing units and are used for sucking or sucking out culture solution through the cover plate. The detector provided by the invention is convenient for detecting the liquid level condition of the solution and is easy to popularize.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of an ELISA plate liquid level detection apparatus provided by an embodiment of the invention;
FIG. 2 is a top view of an ELISA plate level detection apparatus provided in an embodiment of the invention;
FIG. 3 is a schematic cross-sectional view of portion A-A of FIG. 2;
fig. 4 is a top view of a placement part in the elisa plate liquid level detection device according to the embodiment of the present invention;
FIG. 5 is a schematic diagram of a light emitter in an ELISA plate level detection apparatus provided in an embodiment of the invention;
fig. 6 is a schematic diagram of a receiving groove in a detector according to an embodiment of the present invention.
Icon: 1-a placement part; a 2-ray emitter; 11-a housing unit; 111-light holes; 21-a receiving groove; 22-light source; 23-controlling a switch; 24-separator; 25-top plate; 211-darkroom unit; 251-exit aperture.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that, if terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like are used, the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, only for convenience of describing the present invention and simplifying the description, and does not indicate or imply that the indicated apparatus or element must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like, as used herein, are used for descriptive purposes only and are not to be construed as indicating or implying any relative importance.
In the description of the present invention, it should be noted that unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" should be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
As shown in fig. 1, 2 and 3, the device for detecting the liquid level of the elisa plate provided in the present embodiment includes a placement part 1 and a light emitter 2; the placement unit 1 is provided with a plurality of containing units 11, and the plurality of containing units 11 correspond to the micro-reaction cells of the ELISA plate, respectively. Since the micro reaction cells on the 96-well ELISA plate in the prior art are generally arranged in a matrix, the plurality of accommodating units 11 may be arranged in a matrix so that the plurality of accommodating units 11 can accommodate a plurality of micro reaction cells of the ELISA plate. The light emitter 2 is used in a micro reaction cell which generates transmitted light and refracts the transmitted light to a 96-well ELISA plate. The light emitter 2 may include: the housing groove 21, the top plate 25, the plurality of partitions 24, the plurality of light sources 22, and the control switch 23. A plurality of partitions 24 are disposed in the accommodating chamber along a predetermined direction for partitioning the accommodating chamber into a plurality of elongated darkroom units 211, as shown in fig. 6; the light sources 22 are respectively disposed in the darkroom unit 211, and the light sources 22 are disposed along the extending direction of the darkroom unit 211; the top end of each darkroom unit 211 (the top plate 25 is provided with a light outlet 251) which is parallel to the directions of the darkroom units 211, the placement part 1 is arranged at the upper part of the light emitter 2, the bottom of the accommodating unit 11 is provided with a light transmission hole 111, the plurality of light outlet 251 correspond to the light transmission holes 111 at the bottom of the corresponding accommodating unit 11 and are used for projecting the light of the light source 22 into the micro reaction tanks placed in the accommodating unit 11 through the light outlet 251 and the light transmission holes 111 in sequence; the control switch 23 is electrically connected to the plurality of light sources 22, and is used for correspondingly controlling the on/off of the light sources 22.
During detection, the ELISA plate is placed in the accommodating unit 11, the light source 22 in one darkroom unit 211 is turned on, the strip-shaped light source 22 sequentially enters the accommodating unit 11 through the light emitting holes 251 and the light transmitting holes 111, a row (or line) of reflecting surfaces are formed on the liquid level in the micro-reaction tank, and the liquid level condition in the micro-reaction tank can be conveniently observed through the reflecting surfaces. If the same amount of liquid is counted in the micro-reaction tank, the light reflection surface in the micro-reaction tank should be consistent. If no reflecting surface exists in the micro-reaction tanks or the average reflecting surface is lower than the average reflecting surface, the leak holes or the sample adding amount are judged to be inconsistent, and liquid supplementing is needed at the moment until the sample adding amount of the liquid in each micro-reaction tank is consistent. In the detection, different light sources 22 may be turned on sequentially to check the liquid level of the micro reaction cells on the same strip darkroom unit 211 sequentially, or a plurality of light sources 22 may be turned on simultaneously to observe. Therefore, the ELISA plate liquid level detection device provided by the embodiment is convenient to detect the micro reaction tanks of the ELISA plate single column in sequence, and the liquid level judgment condition is accurate. Compared with the detection by the chromogenic reagent in the prior art, when the chromogenic reagent is added, the solution is irreversibly changed, so that the liquid level condition of the corresponding column detected before is easier to influence the liquid level condition of the solution in the next column. Meanwhile, the ELISA plate liquid level detection device provided by the embodiment does not need to add a chromogenic reagent and the like in the micro-reaction tank, and is convenient in detecting the liquid level condition of the solution and easy to popularize.
The above-mentioned placement portions 1 may each be made of an opaque material. The placement part 1 may be made of a material such as light-tight plastic or stainless steel. Alternatively, the inner wall of the housing unit 11 may be provided with a light-blocking layer that is opaque to avoid the influence of light from the outside on the microplate. The light source 22 may be configured as a monochromatic light source 22 to avoid formation of a polychromatic liquid level after light dispersion occurs during light reflection.
Further, the colors of the light sources 22 in adjacent darkroom units 211 are not the same. With the above arrangement, the corresponding light sources 22 in the plurality of darkroom units 211 can be turned on at the same time to observe the liquid level condition of the micro reaction tanks in a plurality of rows on the ELISA plate, so as to improve the detection efficiency.
The plurality of containing units 11 may be provided as: the plurality of accommodating units 11 are arranged in the first direction and the second direction; the first direction is perpendicular to the second direction; the number of the accommodating units 11 arranged in the first direction is greater than the number of the accommodating units 11 in the second direction. In the experiment, the commonly used ELISA plate is a 96-well ELISA plate, and the micro-reaction tank is at most 12 ANGSTROM 8 on the ELISA plate. The micro-reaction tanks can be detachably connected on the support plate of the ELISA plate, so that a plurality of micro-reaction tanks such as 12 ANGSTROM 4 and the like can be placed on the ELISA plate. Therefore, in the first direction, the number of the accommodation units 11 may be set to 12; the number of the accommodation units 11 in the second direction may be set to 8 to meet the detection needs. The second direction is parallel to the extending direction of the strip-shaped darkroom units 211.
For the experiment requiring to detect the same row and the same column, the elisa plate liquid level detection device provided in this embodiment may also be set as: the plurality of accommodating units 11 are arranged in the first direction and the second direction; the number of the containing units 11 arranged in the first direction and the second direction is the same, and the number of the containing units 11 arranged in the first direction and the second direction is the same as the maximum number of the micro reaction cells in the first direction and the second direction of the microplate. For the ELISA plate with 12 ANGSTROM 8, when the liquid level of the micro-reaction tank in the ELISA plate column direction is detected, the ELISA plate can be rotated 90 degrees along the horizontal direction so as to detect the liquid level in the micro-reaction tank in the ELISA plate row direction.
Taking a 96-well ELISA plate commonly used in experiments as an example, as shown in FIG. 4 and FIG. 5, a micro-reaction cell of 12 ANGSTROM 8 can be provided on the 96-well ELISA plate Kong Zuiduo, and therefore, in the ELISA plate liquid level detection apparatus provided in this embodiment, the number of accommodation units 11 in the first direction and the second direction of the plate placement portion 1 is set to 12. After the rows of the ELISA plates are detected, the ELISA plates are rotated by 90 degrees, and then the rows of the ELISA plates can be detected.
Alternatively, the plurality of receiving units 11 are arranged in the first direction and the second direction, and the number of receiving units 11 arranged in the first direction is greater than the number of receiving units 11 in the second direction, the light emitting holes 251 are disposed in the second direction, and the lengths of the plurality of light emitting holes 251 sequentially arranged are greater than or equal to the lengths of the plurality of receiving units 11 arranged in the second direction. After the ELISA plate is placed in the placing part 1 to detect the row, the placing part 1 and the ELISA plate are rotated simultaneously to detect the micro reaction cells in the row direction of the ELISA plate. For example, for a 96-well microplate, the placement portion 1 may be provided with a 12' 8 receiving unit 11. In the first direction, the light-receiving plate is provided with a light-emitting aperture 251 of 12 x 12. After the detection of the column direction of the elisa plate is completed, the placement unit 1 and the elisa plate may be rotated together by 90 degrees, and then the placement unit 1 may be placed on the light emitter 2 to perform the row detection of the elisa plate.
In order to facilitate the placement of the placement part 1 into the upper part of the light emitter 2 such that the light exit hole 251 corresponds to the accommodation unit 11, the light emitter 2 may comprise a stopper part for connection with the placement part 1. Specifically, a convex portion may be provided at an edge of an upper portion of the light emitter 2, and a groove corresponding to the convex portion may be provided at a lower side surface of the placement portion 1, and when the placement portion 1 is placed in the upper portion of the light emitter 2, the convex portion may be corresponding to the groove to rapidly correspond the light-emitting 251 to the accommodation unit 11. At the same time, the provision of the projection and the recess can prevent the placement portion 1 from slipping out from the upper portion of the light emitter 2.
The top plate 25 and the receiving groove 21 may be provided in a detachable connection manner. The above arrangement facilitates replacement of the light source 22 within the light emitter 2.
In order to facilitate the taking of the microplate placed on the housing unit 11, the edge of the placement portion 1 is provided with a recess for forming a gap between the microplate and the recess. Operators can conveniently take the ELISA plate through the gaps.
The light source 22 may be a strip-shaped LED lamp. The diameter of the light outlet hole 251 is 2-4mm. The accommodating unit 11 on the accommodating groove 21 may be provided in a cylindrical shape, and the bottom of the accommodating unit 11 is the same size as the upper opening. The above arrangement is provided to facilitate the irradiation of light into the micro-reaction cell from the bottom of the housing unit 11.
The control switch 23 may be provided in plural, and the plural control switches 23 may be sequentially associated with the light sources 22 in the different darkroom units 211. The control switch 23 includes a brightness control unit, and a brightness control board is electrically connected to the control switch 23 for controlling the brightness of the light source 22.
In summary, in the elisa plate level detection device provided in this embodiment, the placement unit 1 is provided with a plurality of accommodating units 11 arranged in a matrix, and the accommodating units 11 are used for placing micro-reaction tanks of the elisa plate. The bottom of the accommodation unit 11 is provided as a light-transmitting hole 111. The lower part of the placement part 1 is a light emitter 2. The light emitter 2 comprises strip-shaped darkroom units 211, and strip-shaped light sources 22 are arranged in the darkroom units 211. Along the extending direction of the darkroom unit 211, a plurality of light emitting holes 251 are arranged at the top of the darkroom unit 211, and the plurality of light emitting holes 251 respectively correspond to the bottom of the accommodating unit 11. The light generated by the light source 22 irradiates the micro-reaction cells in the accommodating unit 11 through the light outlet 251 and the light transmitting hole 111 to form a refraction surface in the micro-reaction cells, and the liquid level conditions of the plurality of micro-reaction cells in the array direction are judged by the height of the refraction surface. Meanwhile, the control switch 23 respectively controls the light sources 22 corresponding to the array directions, and the array-direction micro reaction tanks can be sequentially detected during detection, so that the detection is convenient and the detection efficiency is high.
The detector provided by the embodiment comprises a cover plate and the ELISA plate liquid level detection device; the cover plate is made of transparent material, and is provided with a plurality of liquid adding holes corresponding to the accommodation units 11, respectively, for sucking or sucking out the culture liquid through the cover plate. After detecting the liquid level in the micro-reaction tanks in the same column, the liquid can be added into the micro-reaction tanks with low liquid level through the liquid adding holes by the liquid transferring gun. Meanwhile, the cover plate is made of transparent materials, so that the liquid level condition in the micro-reaction tank can be conveniently observed. When the liquid is added, the cover plate is covered on the ELISA plate, so that the situation that impurities such as dust enter the micro-reaction tank in the detection and liquid adding process is avoided. The detector provided by the embodiment is convenient in detecting the liquid level condition of the solution and easy to popularize.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (7)
1. The ELISA plate liquid level detection device is characterized by comprising a placement part and a light emitter;
the placing part is provided with a plurality of accommodating units, and the plurality of accommodating units respectively correspond to the micro-reaction tanks of the ELISA plate so that the accommodating units can correspondingly accommodate the plurality of micro-reaction tanks of the ELISA plate;
the light emitter includes: the light source comprises a containing groove, a top plate, a partition plate, a light source and a control switch; the opening end of the accommodating groove is connected with the top plate to form an accommodating cavity; the number of the partition plates and the number of the light sources are multiple; the plurality of baffles are arranged in the accommodating cavity along a preset direction and are used for dividing the accommodating cavity into a plurality of strip-shaped darkroom units; the light sources are respectively arranged in the darkroom unit, and the light sources are arranged along the extending direction of the darkroom unit; the top plate is provided with a plurality of light emitting holes, the light emitting holes respectively correspond to the darkroom units, and the light emitting holes at the top end of each darkroom unit are arranged along the direction parallel to the darkroom units; the placing part is arranged at one side of the top plate far away from the accommodating groove; the light emitting holes are corresponding to the light transmitting holes of the accommodating unit and are used for projecting light emitted by the light source into the micro reaction tank arranged in the accommodating unit through the light emitting holes and the light transmitting holes; so as to observe the liquid level condition in each micro-reaction tank;
the control switch is electrically connected with the light sources and is used for correspondingly controlling the opening and closing of the light sources;
the inner wall of the accommodating unit is provided with an opaque shading layer; the light source is set as a monochromatic light source;
the colors of the light sources in adjacent darkroom units are different;
the light emitter comprises a limiting part connected with the placing part, so that the light emergent hole corresponds to the light transmitting hole of the accommodating unit.
2. The elisa plate liquid level detection apparatus of claim 1 wherein a plurality of the receiving units are arranged in a first direction and a second direction; the first direction and the second direction are perpendicular; the number of the accommodating units arranged in the first direction is greater than the number of the accommodating units in the second direction.
3. The elisa plate liquid level detection apparatus of claim 1 wherein a plurality of the receiving units are arranged in a first direction and a second direction; the first direction and the second direction are perpendicular; the number of the containing units arranged in the first direction and the second direction is the same, and the number of the containing units arranged in the first direction and the second direction is the same as the maximum number of the micro reaction tanks in the first direction and the second direction of the ELISA plate.
4. The ELISA plate level detecting apparatus as set forth in claim 3, wherein the number of said accommodation units in the first direction and the second direction of said placement section is set to 12.
5. The microplate reader of claim 1, wherein the top plate and the holding groove are removably connected.
6. The microplate reader of claim 1, wherein the rim of the placement portion is provided with a recess for forming a gap between the microplate and the recess.
7. A detector comprising a cover plate and an elisa plate level detection device according to any of claims 1-6;
the cover plate is made of transparent materials, and is provided with a plurality of liquid adding holes, and the liquid adding holes respectively correspond to the containing units and are used for sucking or sucking out culture solution through the cover plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810711502.3A CN108562740B (en) | 2018-07-02 | 2018-07-02 | ELISA plate liquid level detection device and detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810711502.3A CN108562740B (en) | 2018-07-02 | 2018-07-02 | ELISA plate liquid level detection device and detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108562740A CN108562740A (en) | 2018-09-21 |
| CN108562740B true CN108562740B (en) | 2024-04-09 |
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