CN109603941A - Micro-fluidic chip system and micro-fluidic chip - Google Patents
Micro-fluidic chip system and micro-fluidic chip Download PDFInfo
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- CN109603941A CN109603941A CN201910026228.0A CN201910026228A CN109603941A CN 109603941 A CN109603941 A CN 109603941A CN 201910026228 A CN201910026228 A CN 201910026228A CN 109603941 A CN109603941 A CN 109603941A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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Abstract
Present disclose provides a kind of micro-fluidic chip system and micro-fluidic chips, belong to chip technology field.The micro-fluidic chip includes substrate, thermochromic liquid crystal layer, flow channel layer and control layer.Wherein, thermochromic liquid crystal layer is oppositely arranged with substrate；Flow channel layer is set to thermochromic liquid crystal layer close to the side of substrate, and has the runner flowed for measuring samples；Control layer is set between substrate and flow channel layer, and control layer includes at least one control unit, and at least one control unit is target control unit, and target control unit includes optoelectronic induction device；Optoelectronic induction device is used to receive the light across thermochromic liquid crystal layer and flow channel layer, and the electric signal that can reflect temperature is generated according to the intensity of light.The micro-fluidic chip is capable of measuring the temperature of measuring samples, and measurement error is smaller.
Description
Technical field
This disclosure relates to chip technology field more particularly to a kind of micro-fluidic chip system and micro-fluidic chip.
Background technique
In recent years, to the research of micro-fluidic chip just in mushroom development.Micro-fluidic chip, which refers to, utilizes subtle processing technology
The minim channel network of solution flowing is fabricated on glass or plastic base, by biology, the sample of chemistry and medical analysis process
The basic operation units such as product preparation, reaction, separation and detection are integrated on chip piece, and are automatically performed analysis overall process.Number
When word micro-fluidic chip works, temperature controls whether accurately to will have a direct impact on experimental result, and the temperature difference is larger to will lead to PCR (polymerization
Enzyme chain reaction) amplification failure；It is also very stringent to the control of temperature in the reaction for having the participations such as antigen, antibody.The prior art
Temp measuring method measurement error it is larger, it is difficult to meet growing requirement of experiment.
Above- mentioned information disclosed in the background technology part are only used for reinforcing the understanding to the background of the disclosure, therefore it can
To include the information not constituted to the prior art known to persons of ordinary skill in the art.
Summary of the invention
The disclosure is designed to provide a kind of micro-fluidic chip system and micro-fluidic chip, can reduce measuring samples temperature
Measurement error.
For achieving the above object, the disclosure adopts the following technical scheme that
According to one aspect of the disclosure, a kind of micro-fluidic chip is provided, including substrate, thermochromic liquid crystal layer, flow channel layer and
Control layer.Wherein: thermochromic liquid crystal layer is oppositely arranged with the substrate；Flow channel layer is set to the thermochromic liquid crystal layer close to the substrate
Side, and there is the runner that flows for measuring samples；Control layer is set between the substrate and the flow channel layer, the control
Layer includes at least one control unit, and at least one described control unit is target control unit, the target control unit
Including optoelectronic induction device；The optoelectronic induction device is for receiving the light across the thermochromic liquid crystal layer and the flow channel layer
Line, and the electric signal that can reflect temperature is generated according to the intensity of the light.
In a kind of exemplary embodiment of the disclosure, optoelectronic induction device is photodiode.
In a kind of exemplary embodiment of the disclosure, the target control unit further include:
First switch device is connect with the optoelectronic induction device, is used to open or closes the optoelectronic induction device.
In a kind of exemplary embodiment of the disclosure, the first switch device is thin film transistor (TFT).
In a kind of exemplary embodiment of the disclosure, the micro-fluidic chip further include:
First transparent substrate, set on the surface of the thermochromic liquid crystal layer far from the substrate；
Second transparent substrate, set on the thermochromic liquid crystal layer close to the surface of the substrate, and with the described first transparent base
Cavity corresponding with the target control unit is formed between plate；
The thermochromic liquid crystal layer limits in the cavity, and is arranged with the target control unit face.
In a kind of exemplary embodiment of the disclosure, the flow channel layer includes:
First hydrophobic layer is set between the control layer and the thermochromic liquid crystal layer；
Second hydrophobic layer, be set between the control layer and the thermochromic liquid crystal layer, and with the first hydrophobic layer face
Setting；
Frame, be set between first hydrophobic layer and second hydrophobic layer, the runner be first hydrophobic layer,
The space that second hydrophobic layer and the frame surround.
In a kind of exemplary embodiment of the disclosure, the micro-fluidic chip further include:
Transparency conducting layer is set between second transparent substrate and first hydrophobic layer；
Each described control unit includes:
Driving unit, including driving electrodes and second switch device；The driving electrodes and the transparency conducting layer face
Setting, for forming the electric field for being used to drive the measuring samples mobile with the transparency conducting layer；The second switch device
For making the driving electrodes on/off electricity.
In a kind of exemplary embodiment of the disclosure, the control layer includes temperature detection area, the target control list
The quantity of member is multiple, and array distribution is in the temperature detection area.
In a kind of exemplary embodiment of the disclosure, outside orthographic projection of the thermochromic liquid crystal layer on the control layer
Edge is located within the outer rim in the temperature detection area.
According to another aspect of the disclosure, a kind of micro-fluidic chip system is provided, comprising:
Micro-fluidic chip described in any of the above embodiments；
Light source, set on the side of the thermochromic liquid crystal layer far from the flow channel layer, for emitting to the thermochromic liquid crystal layer
Light.
The micro-fluidic chip system and micro-fluidic chip that the disclosure provides, when in use, to micro-fluidic chip flow channel layer
It is passed through measuring samples in runner, and light is emitted to thermochromic liquid crystal layer by light source；The temperature of measuring samples will affect thermocolour liquid
The color of crystal layer changes the light transmittance of thermochromic liquid crystal layer, and then it is saturating after thermochromic liquid crystal layer and flow channel layer to influence light
Cross the intensity of light；Optoelectronic induction device, which receives, penetrates light, and generates corresponding electric signal according to the intensity through light；To electric signal
Carrying out analysis can be obtained the temperature value of measuring samples.The micro-fluidic chip is capable of measuring the temperature of measuring samples, and measurement error
It is smaller.
Detailed description of the invention
The drawings herein are incorporated into the specification and forms part of this specification, and shows the implementation for meeting the disclosure
Example, and together with specification for explaining the principles of this disclosure.It should be evident that the accompanying drawings in the following description is only the disclosure
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is the partial structural diagram of disclosure embodiment micro-fluidic chip.
Fig. 2 is the overall structure diagram of disclosure embodiment micro-fluidic chip.
Fig. 3 is the schematic diagram of disclosure embodiment micro-fluidic chip optical path.
Fig. 4 is the detection schematic diagram of the single droplet of disclosure embodiment micro-fluidic chip.
Fig. 5 is the detection schematic diagram of the multiple droplets of disclosure embodiment micro-fluidic chip.
Fig. 6 is the detection schematic diagram of the single big drop of disclosure embodiment micro-fluidic chip.
In figure: 100, measuring samples；1, substrate；2, thermochromic liquid crystal layer；3, flow channel layer；30, runner；31, frame；32,
One hydrophobic layer；33, the second hydrophobic layer；4, control layer；40, optoelectronic induction device；41, first switch device；410, the first source electrode；
411, the first drain electrode；412, the first active layer；413, first grid insulating layer；414, first grid；42, driving electrodes；43,
Two switching devices；430, the second source electrode；431, the second drain electrode；432, the second active layer；433, second grid insulating layer；434,
Two grids；5, the first transparent substrate；6, the second transparent substrate；7, transparency conducting layer；8, temperature detection area；9, sample feeding area；
10, reagent sample introduction zone；11, Concentration Testing area；12, sample reagent mixed zone；13, sample detection zone；14, collecting region；15, first
Region；16, second area；17, light source.
Specific embodiment
Example embodiment is described more fully with reference to the drawings.However, example embodiment can be with a variety of shapes
Formula is implemented, and is not understood as limited to embodiment set forth herein；On the contrary, thesing embodiments are provided so that the present invention will
Fully and completely, and by the design of example embodiment comprehensively it is communicated to those skilled in the art.Identical attached drawing in figure
Label indicates same or similar structure, thus the detailed description that will omit them.
Although the term of relativity, such as "upper" "lower" is used to describe a component of icon for another in this specification
The relativeness of one component, but these terms are in this manual merely for convenient, for example, with reference to the accompanying drawings described in show
The direction of example.It is appreciated that, if making it turn upside down the device overturning of icon, the component described in "upper" will
As the component in "lower".When certain structure is at other structures "upper", it is possible to refer to that certain structural integrity is formed in other structures
On, or refer to that certain structure is " direct " and be arranged in other structures, or refer to that certain structure is arranged by the way that another structure is " indirect " in other knots
On structure.
In the figure for clarity, may be exaggerated the thickness of region and layer.Identical appended drawing reference indicates identical in figure
Or similar structure, thus the detailed description that them will be omitted.
Term "one", " one ", "the", " described " to indicate there are one or more elements/component part/etc.；With
Language " comprising " and " having " is to indicate the open meaning being included and refer to element/composition portion in addition to listing
Also may be present except divide/waiting other element/component part/etc.；Term " first ", " second " only use as label, are not
Quantity limitation to its object.
In the related technology, the temp measuring method of micro-fluidic chip is that thermocouple is arranged in micro-fluidic chip side to measure temperature
The temperature of detection zone measuring samples 100, thermocouple detect temperature by way of heat transfer, have hysteresis quality, so that measurement misses
Difference is larger, and needs specially to install thermocouple when detecting, and complicated operation.
As shown in Figure 1, disclosure embodiment provides a kind of micro-fluidic chip, it can be used for detecting biology, chemistry and medicine
The temperature value of the measuring samples 100 of analytic process.For example, measuring samples 100 can be in biological tissue in solution
Cell, protein, chromosome etc..Certainly, measuring samples 100 can also be other liquids, will not enumerate herein.
Disclosure embodiment micro-fluidic chip includes substrate 1, thermochromic liquid crystal layer 2, flow channel layer 3 and control layer 4.Wherein,
Thermochromic liquid crystal layer 2 is oppositely arranged with substrate 1；
Flow channel layer 3 is set to thermochromic liquid crystal layer 2 close to the side of substrate 1, and has the runner flowed for measuring samples 100
30；
Control layer 4 is set between substrate 1 and flow channel layer 3, and control layer 4 includes at least one control unit, and at least one
Control unit is target control unit, and target control unit includes optoelectronic induction device 40；Optoelectronic induction device 40 is for receiving
Across the light of thermochromic liquid crystal layer 2 and flow channel layer 3, and the electric signal that can reflect temperature is generated according to the intensity of light.
The micro-fluidic chip system and micro-fluidic chip that the disclosure provides, when in use, to micro-fluidic chip flow channel layer 3
It is passed through measuring samples 100 in runner 30, and light is emitted to thermochromic liquid crystal layer 2 by light source 17；The temperature meeting of measuring samples 100
The color for influencing thermochromic liquid crystal layer 2, changes the light transmittance of thermochromic liquid crystal layer 2, and then influences light and pass through 2 He of thermochromic liquid crystal layer
The intensity through light after flow channel layer 3；Optoelectronic induction device 40, which receives, penetrates light, and is generated accordingly according to the intensity through light
Electric signal；Carrying out analysis to electric signal can be obtained the temperature value of measuring samples 100.The micro-fluidic chip is capable of measuring measuring samples
100 temperature, and measurement error is smaller.
Each component of the micro-fluidic chip provided with reference to the accompanying drawing disclosure embodiment is described in detail:
As shown in Figure 1, the shape of substrate 1 can be rectangle, circle or other shapes, will not enumerate herein.
As shown in Figure 1, thermochromic liquid crystal layer 2 can have thermochromic liquid crystal, in thermochromic liquid crystal temperature change, thermochromic liquid crystal it is saturating
Light rate changes, and the intensity through light across thermochromic liquid crystal layer 2 changes.Thermochromic liquid crystal layer 2 opposite with substrate 1 can be set
It sets, projection of the thermochromic liquid crystal layer 2 on substrate 1 is located within the edge of substrate 1.
For the ease of installing thermochromic liquid crystal layer 2, in one embodiment, disclosure embodiment micro-fluidic chip can also be wrapped
The first transparent substrate 5 and the second transparent substrate 6 are included, cavity can be formed between the second transparent substrate 6 and the first transparent substrate 5, it is empty
Chamber is used to accommodate thermochromic liquid crystal layer 2.Wherein:
First transparent substrate 5 can be transparent glass or crystalline ceramics, it is of course also possible to be other transparent materials, this
Place will not enumerate.Second transparent substrate 6 can be cured transparent casting glue, have the excellent properties such as moisture-proof, insulation.When
So, the second transparent substrate 6 is also possible to transparent glass or crystalline ceramics etc., will not enumerate herein.First transparent substrate 5
It is not particularly limited herein with the thickness of the second transparent substrate 6.
Accommodation space of the cavity as thermochromic liquid crystal layer 2 can open up on the surface of substrate 1 in the first transparent substrate 5
Groove, or groove is opened up on the second surface of the transparent substrate 6 far from substrate 1, or saturating in the first transparent substrate 5 and second
Groove is opened up on the adjacent surface of bright substrate 6, the first transparent substrate 5 and the second transparent substrate 6 are bonded and form cavity.Groove
Depth can be greater than or equal to the thickness of thermochromic liquid crystal layer 2, so that groove can accommodate thermochromic liquid crystal layer 2.It should be noted that cavity
It both can be coherent cavity, or incoherent cavity.When cavity is discontinuous, it can not only reduce and open up cavity
Workload can also save thermochromic liquid crystal layer 2, and finally reduce the cost of micro-fluidic chip.
As shown in Figure 1, flow channel layer 3 can have runner 30, measuring samples 100 can be flowed along runner 30.
Runner 30 has inlet and outlet, and measuring samples 100 enter micro-fluidic chip through import, and miniflow is discharged through outlet
Control chip.The shape of inlet and outlet can be to be rectangular or round etc., and the specific size of inlet and outlet does not make special limit herein
It is fixed.In addition, the position of inlet and outlet, which is subject to, facilitates arrangement, it is not described in detail herein.
Measuring samples 100 leak in order to prevent, and in one embodiment, the flow channel layer 3 of disclosure embodiment can wrap
The first hydrophobic layer 32, the second hydrophobic layer 33 and frame 31 are included, and three can surround runner 30, in which:
First hydrophobic layer 32 and the second hydrophobic layer 33 can be set between control layer 4 and thermochromic liquid crystal layer 2, and the two face
Setting, so that measuring samples 100 can be set to therebetween.For example, the material of the first hydrophobic layer 32 and the second hydrophobic layer 33
It can be Teflon etc., will not enumerate herein.First hydrophobic layer 32 and the second hydrophobic layer 33 can pass through the modes shape such as spin coating
At being not described in detail herein.
Frame 31 can be set between the first hydrophobic layer 32 and the second hydrophobic layer 33, and hydrophobic with the first hydrophobic layer 32 and second
Layer 33 forms runner 30, for the flowing of measuring samples 100.For example, the shape of frame 31 can be rectangle, circle or other shapes
Shape, and it can be surrounded by multiple mouldings are end to end, and be set to the outer edge of micro-fluidic chip, each moulding can be blocked by bonding
The mode of connecing connects, and is also possible to integral structure.Meanwhile settable multiple support columns in runner 30, so that support first is hydrophobic
Layer 23 and the second hydrophobic layer 33, prevent from collapsing.
In another embodiment, flow channel layer 3 also may not include the first hydrophobic layer 32 and the second hydrophobic layer 33, and frame 31 can
Between the second transparent substrate 6 and control layer 4, frame 31, the second transparent substrate 6 and control layer 4 can also surround runner 30, with
It is flowed for measuring samples 100.
It should be noted that the inner wall of runner 30 can shape in order to reduce resistance of the measuring samples 100 when runner 30 flows
At there is lubricant layer, the material of lubricant layer can be solid mineral oil, paraffin or other lubriation materials, can not only reduce promotion to
The power for the driving electrodes 42 that sample product 100 flow, can also accelerate the detection speed of 100 temperature of measuring samples.
As shown in Figure 1, control layer 4 can be set between substrate 1 and flow channel layer 3, control layer 4 may include control unit, control
The quantity of unit can be one, two, three or more, and at least one control unit is target control unit.
Target control unit may include optoelectronic induction device 40, optoelectronic induction device 40 can for PIN photodiode or
Other devices that can will carry out photoelectric conversion, optoelectronic induction device 40 is corresponding with thermochromic liquid crystal layer 2, for receiving across thermocolour liquid
The transmission light of crystal layer 2 and flow channel layer 3, and the electric signal that can reflect 100 temperature of measuring samples is generated according to the intensity through light, it should
Electric signal changes with the variation of the intensity of light, so as to can determine the temperature value of measuring samples 100 according to the electric signal.
Target control unit may also include first switch device 41, and first switch device 41 and optoelectronic induction device 40 connect
It connects, is used to open or closes optoelectronic induction device 40.
For example, as shown in Figure 1, first switch device 41 can be thin film transistor (TFT) (TFT), which can
It may include the 411, first active layer 412 of the first source electrode 410, first drain electrode, first grid insulation for bottom gate thin film transistor
Layer 413 and first grid 414, first grid 414 are set to substrate 1 close to the surface of thermochromic liquid crystal layer 2, first grid insulating layer
413 covering first grids 414, the first active layer 412 are set to surface of the first grid insulating layer 413 far from substrate 1, the first source electrode
410 and first drain electrode 411 be set to the first surface of the active layer 412 far from first grid insulating layer 413, and the first source electrode 410 with
Optoelectronic induction device 40 connects, and is powered or powers off for controlling optoelectronic induction device 40.
Certainly, first switch device 41 can also be top gate type thin film transistor or other switching devices, not another herein
One enumerates.
For the ease of determining the temperature value of measuring samples 100, the micro-fluidic core of disclosure embodiment according to electric signal
Piece may include processor, and processor is connect with optoelectronic induction device 40, for receiving the electric signal of the sending of optoelectronic induction device 40,
And the temperature value of measuring samples 100 is determined by the corresponding relationship between preset electric signal and temperature.
As shown in Fig. 2, the micro-fluidic chip of disclosure embodiment may include temperature detection area 8, target control unit
Quantity is multiple, and can array distribution in temperature detection area 8, orthographic projection of the thermochromic liquid crystal layer on substrate 1 is located at temperature and examines
It surveys in area 8.Temperature detection area 8 may include multiple subregions of array distribution, and the size of all subregion is identical.For example,
In temperature detection area 8 quantity of subregion can be 16, as shown in figure 4, and rectangular array be distributed as first row, secondary series,
Third column and the 4th column, wherein the subregion of first row successively can be labeled as A1~A4, the subregion of secondary series can be followed successively by B1
~B4, tertial subregion can successively be labeled as C1~C4, and the subregion of the 4th column can successively be labeled as D1~D4, every height
Target control unit is equipped in region.There are when measuring samples 100 in any subregion, the mesh in the subregion can be passed through
Mark the temperature of control unit detection measuring samples 100.
The micro-fluidic chip of disclosure embodiment may also include transparency conducting layer 7, and each control unit further includes driving
Unit, that is to say, that each target control unit also includes driving unit, and it is wet that driving unit and transparency conducting layer 7 are based on dielectric
Moisten the movement that principle realizes measuring samples 100, specifically:
Transparency conducting layer 7 can be set between the second transparent substrate 6 and the first hydrophobic layer 32, and the material of transparency conducting layer 7
It can be transparent conductive materials such as ITO (tin indium oxides), will not enumerate herein.
Each driving unit may include driving electrodes 42 and second switch device 43.Wherein, driving electrodes 42 are led with transparent
The setting of 7 face of electric layer will form electric field after driving electrodes 42 are powered between driving electrodes 42 and transparency conducting layer 7, droplet-like
The contact angle of 100 Part portions of measuring samples reduces, and so that the local shape of the measuring samples 100 of droplet-like is changed, and draw
Play internal pressure difference；By making the driving electrodes 42 of adjacent two driving unit successively be powered, and the liquid under the driving of pressure difference
The measuring samples 100 of drop-wise move between the position of adjacent two driving electrodes 42 of correspondence.It is multiple in the quantity of driving unit, and
When along a preset path permutations, the to be checked of droplet-like can be made by making the driving electrodes 42 of each driving unit successively be powered
Sample 100 is moved along the path that driving unit is formed.
Second switch device 43 can be connected with driving electrodes 42, for driving electrodes 42 be powered or power off, with open or
Driving electrodes 42 are closed, and second switch device 43 can be controlled independently, respectively to drive list according to preset sequential control
The driving electrodes 42 on/off electricity of member.
For example, as shown in Figure 1, second switch device 43 can be thin film transistor (TFT) (TFT), which can
It may include the 431, second active layer 432 of the second source electrode 430, second drain electrode, second grid insulation for bottom gate thin film transistor
Layer 433 and second grid 434, second grid 434 are set to substrate 1 close to the surface of thermochromic liquid crystal layer 2, second grid insulating layer
433 covering second grids 434, the second active layer 432 are set to surface of the second grid insulating layer 433 far from substrate 1, the second source electrode
430 and second drain electrode 431 be set to the second surface of the active layer 432 far from second grid insulating layer 433, and second drain electrode 431 with
Driving electrodes 42 connect, and are powered or power off for controlling driving electrodes 42.
Certainly, second switch device 43 can also be top gate type thin film transistor or other switching devices, not another herein
One enumerates.
For example, in temperature detection area 8, when measuring samples 100 are the drop of small volume, single droplet is being controlled
The orthographic projection of preparative layer 4 may be slightly larger than the area of single control unit, as shown in Figure 4.When temperature detection area 8 is set, there are three droplets
When, as shown in figure 5, the second switch device 43 of the control unit in controllable C1 subregion opens corresponding driving electrodes 42,
Droplet is pushed to be moved to C2 subregion from C1 subregion.At this point, B2 subregion, C2 subregion and C3 subregion can be controlled again
Driving electrodes 42 in control unit act, and three droplets is pushed to move towards, and three droplets permeate a big drop,
As shown in Figure 6.Control B2 subregion control unit, C2 subregion control unit, B3 subregion control unit and C3 subregion control
First switch device 41 in unit processed opens corresponding optoelectronic induction device 40, can carry out thermometric to big drop.In conclusion
The micro-fluidic chip can realize any position in temperature detection area 8, multiple samples, different volumes measuring samples 100 detection,
Enhance the flexibility and efficiency of micro-fluidic chip detection.
As shown in Fig. 2, the micro-fluidic chip may also include sample feeding area 9, reagent sample introduction zone 10, Concentration Testing area 11,
Sample reagent mixed zone 12, sample detection zone 13 and collecting region 14.Wherein, sample feeding area 9 and reagent sample introduction zone 10 are set to temperature
Spend the same side of detection zone 8；Concentration Testing area 11 can be set to side of the temperature detection area 8 far from sample feeding area 9；Sample reagent
Mixed zone 12 can be set to side of the temperature detection area 8 far from Concentration Testing area 11；Sample detection zone 13 can be mixed set on sample reagent
Close side of the area 12 far from temperature detection area 8；Collecting region 14 can be set to sample reagent mixed zone 12 far from sample detection zone 13
Side.
Sample feeding area 9, reagent sample introduction zone 10, Concentration Testing area 11, sample reagent mixed zone 12, sample detection zone 13 are equal
Including multiple control units in array distribution, the second switch of driving unit in each control unit is controlled according to predesigned order
Device 43 opens corresponding driving electrodes 42, and measuring samples 100 can be made in Concentration Testing area 11, sample reagent mixed zone 12, sample
It is moved between product detection zone 13, specific moving process can refer to the movement of 8 measuring samples of temperature detection, and details are not described herein again.
Sample feeding area 9 provides channel into micro-fluidic chip for measuring samples 100, and reagent sample introduction zone 10 is reagent entrance
Micro-fluidic chip provides channel.Reagent can be catalyst, for optimizing the preparations of measuring samples, reaction, separation and detecting
Journey is not described in detail herein.
Concentration Testing area 11 can be divided into two parts, and a part is connect with sample feeding area 9, for detecting measuring samples 100
Concentration, another part is connect with reagent sample introduction zone 10, for the concentration of detection reagent.Measuring samples in Concentration Testing area 11
100 movement can realize that concrete principle can refer to measuring samples in temperature detection area by driving unit and transparency conducting layer 7
100 moving process, details are not described herein again.
The quantity of the target control unit in Concentration Testing area 11 is multiple, and can array distribution in Concentration Testing area 11,
As shown in Figure 2.Since the concentration influence of measuring samples 100 penetrates the intensity of light, thus available targets control unit test sample
Concentration, the structure of target control unit can be consistent with the target control unit in temperature detection area, and this will not be detailed here.Meanwhile
In order to avoid thermochromic liquid crystal layer 2 influences Concentration Testing, setting thermochromic liquid crystal floor 2 is not needed in Concentration Testing area 11.When detecting,
In Concentration Testing area 11, light sequentially passes through the first transparent substrate 5, the second transparent substrate 6 and flow channel layer 3, photoelectric sensor
The reception of part 40 penetrates light, generates the electric signal of reflection concentration further according to the intensity for penetrating light, the electric signal with light intensity
Change and change, so as to can determine the concentration value of measuring samples 100 according to the electric signal.
It should be noted that since what the concentration of measuring samples 100 will affect that optoelectronic induction device 40 receives penetrates light
Intensity so that there are certain journeys for the detection temperature and actual temperature of the measuring samples 100 detected by the micro-fluidic chip
The deviation of degree.Therefore, the actual temperature that the measuring samples 100 of multiple preset concentrations are measured in test can be first passed through in advance, and is passed through
The micro-fluidic chip obtains the detection temperature of the measuring samples 100 of multiple preset concentrations, and according to each actual temperature and detection
Temperature determines the deviation between the actual temperature under each preset concentration and detection temperature.In making for the micro-fluidic chip
With in the process, measuring samples 100 detect concentration through Concentration Testing area 11, and after temperature detection area 8 detects temperature, then tie
It closes deviation and compensates and export the actual temperature of measuring samples 100 to detection temperature, so that the thermometric of the micro-fluidic chip
It is more accurate.
For example, the temperature and the detection of Concentration Testing area 11 that temperature detection area 8 detects can be obtained by processor
The concentration of measuring samples 100 out, and determine deviation corresponding with the concentration, then, according to the deviation to the temperature value
Compensate and export the actual temperature of measuring samples 100.
Sample reagent mixed zone 12 for realizing reagent and measuring samples 100 mixing.Sample reagent mixed zone 12 it is each
A driving unit driving reagent and measuring samples 100 move towards, and realize the mixing of the two.Reagent and measuring samples 100
Movement can realize that concrete principle can refer to measuring samples 100 in temperature detection area by driving unit and transparency conducting layer 7
Moving process, details are not described herein again.
Sample detection zone 13 can be arranged in the sample detection zone 13 for examining for realizing the detection of measuring samples 100
The type for the parameter selection detection device surveying the detection device of measuring samples 100, and detecting as needed.For example, the inspection
Surveying device can be the related detection devices etc. of pH sensor part or integrated enzyme reaction, and detector is arranged in corresponding position
Part is not described in detail herein.
Collecting region 14 is discharged micro-fluidic chip for measuring samples 100 and provides channel, is not described in detail herein.
The micro-fluidic chip of disclosure embodiment may also include first area 15 and second area 16,15 He of first area
Multiple control units of array distribution can be equipped in second area 16, and corresponding with transparency conducting layer 7；First area 15 connects
Concentration Testing area 11 and collecting region 14, second area 16 connect Concentration Testing area 11 and collecting region 14.
In first area 15 and second area 16 each control unit can by its driving unit drive can measuring samples 100
Moved along first area 15 and second area 16, concrete principle can refer to being moved through for measuring samples 100 in temperature detection area
Journey, details are not described herein again.It can make measuring samples 100 can obstructed excessive detection zone 8, sample reagent mixed zone 12 and sample as a result,
Detection zone 13, and collecting region 14 can be moved directly to through first area 15, it is then exhausted from micro-fluidic chip.Pass sequentially through reagent not
Detection zone 8, sample reagent mixed zone 12 and sample detection zone 13 are spent, collecting region 14 can be moved directly to through second area 16, then
Micro-fluidic chip is discharged.
Disclosure embodiment also provides a kind of micro-fluidic chip system, which may include 17 He of light source
The micro-fluidic chip of any of the above-described embodiment, light source 17 are set to side of the thermochromic liquid crystal layer 2 far from flow channel layer 3, are used for heat
Dichroic liquid crystal layer 2 emits light.
For example, light source 17 can be the LED being fixed on workbench or laser etc., will not enumerate herein.
The wavelength for the light that light source 17 emits can be selected according to the material of thermochromic liquid crystal layer 2.In addition, can also be in light source 17 and miniflow
Optical filter is set between control chip, to filter out the light of the wavelength other than specific wavelength, so that the light of specific wavelength only be made to shine
It is incident upon micro-fluidic chip.
As shown in figure 3, light is irradiated on thermochromic liquid crystal layer 2 with incident angle α, α can be zero, i.e. light vertical irradiation.
Certainly, α is also possible to acute angle, and light irradiation thermochromic liquid crystal layer 2 is simultaneously divided into reflected light and refraction light two parts, and refraction light passes through heat
Dichroic liquid crystal layer 2 and flow channel layer 3, and received by optoelectronic induction device 40.
Those skilled in the art after considering the specification and implementing the invention disclosed here, will readily occur to its of the disclosure
Its embodiment.This application is intended to cover any variations, uses, or adaptations of the disclosure, these modifications, purposes or
Person's adaptive change follows the general principles of this disclosure and including the undocumented common knowledge in the art of the disclosure
Or conventional techniques.The description and examples are only to be considered as illustrative, and the true scope and spirit of the disclosure are by appended
Claim is pointed out.
Claims (10)
1. a kind of micro-fluidic chip characterized by comprising
Substrate；
Thermochromic liquid crystal layer is oppositely arranged with the substrate；
Flow channel layer set on the thermochromic liquid crystal layer close to the side of the substrate, and has the runner flowed for measuring samples；
Control layer is set between the substrate and the flow channel layer, and the control layer includes at least one control unit, and at least
One described control unit is target control unit, and the target control unit includes optoelectronic induction device；The optoelectronic induction
Device is for receiving the light across the thermochromic liquid crystal layer and the flow channel layer, and being generated according to the intensity of the light can be anti-
Reflect the electric signal of temperature.
2. micro-fluidic chip according to claim 1, which is characterized in that the optoelectronic induction device is photodiode.
3. micro-fluidic chip according to claim 1, which is characterized in that the target control unit further include:
First switch device is connect with the optoelectronic induction device, is used to open or closes the optoelectronic induction device.
4. micro-fluidic chip according to claim 3, which is characterized in that the first switch device is thin film transistor (TFT).
5. micro-fluidic chip according to claim 1, which is characterized in that the micro-fluidic chip further include:
First transparent substrate, set on the surface of the thermochromic liquid crystal layer far from the substrate；
Second transparent substrate, set on the thermochromic liquid crystal layer close to the surface of the substrate, and with first transparent substrate it
Between be formed with cavity corresponding with the target control unit；
The thermochromic liquid crystal layer limits in the cavity, and is arranged with the target control unit face.
6. micro-fluidic chip according to claim 5, which is characterized in that the flow channel layer includes:
First hydrophobic layer is set between the control layer and the thermochromic liquid crystal layer；
Second hydrophobic layer is set between the control layer and the thermochromic liquid crystal layer, and is arranged with the first hydrophobic layer face；
Frame is set between first hydrophobic layer and second hydrophobic layer, and the runner is first hydrophobic layer, described
The space that second hydrophobic layer and the frame surround.
7. micro-fluidic chip according to claim 6, which is characterized in that the micro-fluidic chip further include:
Transparency conducting layer is set between second transparent substrate and first hydrophobic layer；
Each described control unit includes:
Driving unit, including driving electrodes and second switch device；The driving electrodes and the transparency conducting layer face are arranged,
For forming the electric field for being used to drive the measuring samples mobile with the transparency conducting layer；The second switch device is for making
The driving electrodes on/off electricity.
8. micro-fluidic chip according to claim 1, which is characterized in that the control layer includes temperature detection area, described
The quantity of target control unit is multiple, and array distribution is in the temperature detection area.
9. micro-fluidic chip according to claim 8, which is characterized in that the thermochromic liquid crystal layer is on the control layer
The outer rim of orthographic projection is located within the outer rim in the temperature detection area.
10. a kind of micro-fluidic chip system characterized by comprising
Micro-fluidic chip according to any one of claims 1 to 9；
Light source, set on the side of the thermochromic liquid crystal layer far from the flow channel layer, for emitting light to the thermochromic liquid crystal layer.
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| CN110523450A (en) * | 2019-09-30 | 2019-12-03 | 京东方科技集团股份有限公司 | A kind of micro-fluidic substrate, micro-fluidic chip, microfluidic system and detection method |
| CN110597328A (en) * | 2019-09-18 | 2019-12-20 | 重庆大学 | Flow cooperative control system based on liquid crystal temperature control micro valve |
| CN112138729A (en) * | 2019-06-26 | 2020-12-29 | 京东方科技集团股份有限公司 | Sampling structure, seal structure and detection subassembly |
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