CN109603941B - Micro-fluidic chip system and micro-fluidic chip - Google Patents
Micro-fluidic chip system and micro-fluidic chip Download PDFInfo
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- CN109603941B CN109603941B CN201910026228.0A CN201910026228A CN109603941B CN 109603941 B CN109603941 B CN 109603941B CN 201910026228 A CN201910026228 A CN 201910026228A CN 109603941 B CN109603941 B CN 109603941B
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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Abstract
The disclosure provides a micro-fluidic chip system and a micro-fluidic chip, and belongs to the technical field of chips. The micro-fluidic chip comprises a substrate, a thermochromatic liquid crystal layer, a flow channel layer and a control layer. Wherein, the thermochromatic liquid crystal layer is arranged opposite to the substrate; the flow channel layer is arranged on one side of the thermochromatic liquid crystal layer close to the substrate and is provided with a flow channel for flowing of a sample to be detected; the control layer is arranged between the substrate and the flow channel layer and comprises at least one control unit, the at least one control unit is a target control unit, and the target control unit comprises a photoelectric sensing device; the photoelectric sensing device is used for receiving light rays passing through the thermochromatic liquid crystal layer and the flow channel layer and generating an electric signal capable of reflecting temperature according to the intensity of the light rays. The micro-fluidic chip can measure the temperature of a sample to be detected, and the measurement error is small.
Description
Technical Field
The present disclosure relates to the field of chip technology, and in particular, to a microfluidic chip system and a microfluidic chip.
Background
In recent years, research on microfluidic chips has been vigorously conducted. The micro-fluidic chip is a micro-channel network which is manufactured on a glass or plastic substrate by utilizing a micro-processing technology and flows with solution, basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis are integrated on one chip, and the whole analysis process is automatically completed. When the digital microfluidic chip works, whether temperature control is accurate or not directly influences an experimental result, and PCR (polymerase chain reaction) amplification failure can be caused by large temperature difference; in reactions involving antigens, antibodies, and the like, temperature control is also critical. The temperature measurement method in the prior art has large measurement error and is difficult to meet the increasing experimental requirements.
The above information disclosed in the background section is only for enhancement of understanding of the background of the present disclosure and therefore it may contain information that does not constitute prior art that is known to a person of ordinary skill in the art.
Disclosure of Invention
The disclosed object is to provide a micro-fluidic chip system and a micro-fluidic chip, which can reduce the measurement error of the temperature of a sample to be detected.
In order to achieve the purpose, the technical scheme adopted by the disclosure is as follows:
according to one aspect of the present disclosure, there is provided a microfluidic chip including a substrate, a thermochromic liquid crystal layer, a flow channel layer, and a control layer. Wherein: the thermochromatic liquid crystal layer is arranged opposite to the substrate; the flow channel layer is arranged on one side of the thermochromatic liquid crystal layer close to the substrate and is provided with a flow channel for flowing of a sample to be detected; the control layer is arranged between the substrate and the flow channel layer and comprises at least one control unit, the at least one control unit is a target control unit, and the target control unit comprises a photoelectric sensing device; the photoelectric sensing device is used for receiving the light rays passing through the thermochromatic liquid crystal layer and the flow channel layer and generating an electric signal capable of reflecting the temperature according to the intensity of the light rays.
In one exemplary embodiment of the present disclosure, the photo-sensing device is a photodiode.
In an exemplary embodiment of the present disclosure, the target control unit further includes:
and the first switching device is connected with the photoelectric sensing device and used for switching on or switching off the photoelectric sensing device.
In an exemplary embodiment of the present disclosure, the first switching device is a thin film transistor.
In an exemplary embodiment of the present disclosure, the microfluidic chip further includes:
the first transparent substrate is arranged on the surface of the thermochromatic liquid crystal layer far away from the substrate;
the second transparent substrate is arranged on the surface, close to the substrate, of the thermochromatic liquid crystal layer, and a cavity corresponding to the target control unit is formed between the second transparent substrate and the first transparent substrate;
the thermochromatic liquid crystal layer is limited in the cavity and is arranged opposite to the target control unit.
In an exemplary embodiment of the present disclosure, the flow channel layer includes:
the first hydrophobic layer is arranged between the control layer and the thermochromatic liquid crystal layer;
the second hydrophobic layer is arranged between the control layer and the thermochromatic liquid crystal layer and is opposite to the first hydrophobic layer;
the frame is arranged between the first hydrophobic layer and the second hydrophobic layer, and the flow channel is a space surrounded by the first hydrophobic layer, the second hydrophobic layer and the frame.
In an exemplary embodiment of the present disclosure, the microfluidic chip further includes:
the transparent conducting layer is arranged between the second transparent substrate and the first hydrophobic layer;
each of the control units includes:
a driving unit including a driving electrode and a second switching device; the driving electrode is arranged opposite to the transparent conducting layer and is used for forming an electric field for driving the sample to be detected to move with the transparent conducting layer; the second switching device is used to turn on/off the driving electrode.
In an exemplary embodiment of the present disclosure, the control layer includes a temperature detection area, the target control unit is plural in number, and the array is distributed in the temperature detection area.
In an exemplary embodiment of the present disclosure, an outer edge of an orthographic projection of the thermochromic liquid crystal layer on the control layer is located inside an outer edge of the temperature detection region.
According to another aspect of the present disclosure, there is provided a microfluidic chip system comprising:
the microfluidic chip of any one of the above;
the light source is arranged on one side, away from the flow channel layer, of the thermochromatic liquid crystal layer and used for emitting light to the thermochromatic liquid crystal layer.
When the micro-fluidic chip system and the micro-fluidic chip are used, a sample to be detected is introduced into a flow channel of a flow channel layer of the micro-fluidic chip, and light is emitted to a thermochromatic liquid crystal layer through a light source; the temperature of the sample to be detected can influence the color of the thermochromatic liquid crystal layer, so that the light transmittance of the thermochromatic liquid crystal layer is changed, and the intensity of transmitted light after the light passes through the thermochromatic liquid crystal layer and the flow channel layer is further influenced; the photoelectric sensing device receives the transmitted light and generates a corresponding electric signal according to the intensity of the transmitted light; and analyzing the electric signal to obtain the temperature value of the sample to be detected. The micro-fluidic chip can measure the temperature of a sample to be detected, and the measurement error is small.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure. It is to be understood that the drawings in the following description are merely exemplary of the disclosure, and that other drawings may be derived from those drawings by one of ordinary skill in the art without the exercise of inventive faculty.
Fig. 1 is a schematic partial structure diagram of a microfluidic chip according to an embodiment of the present disclosure.
Fig. 2 is a schematic diagram of an overall structure of a microfluidic chip according to an embodiment of the present disclosure.
Fig. 3 is a schematic diagram of an optical path of a microfluidic chip according to an embodiment of the present disclosure.
Fig. 4 is a schematic diagram of detection of a single droplet of a microfluidic chip according to an embodiment of the present disclosure.
Fig. 5 is a schematic diagram of detection of a plurality of small droplets of a microfluidic chip according to an embodiment of the present disclosure.
Fig. 6 is a schematic diagram of detection of a single large droplet of a microfluidic chip according to an embodiment of the present disclosure.
In the figure: 100. a sample to be detected; 1. a substrate; 2. a thermochromic liquid crystal layer; 3. a flow channel layer; 30. a flow channel; 31. a frame; 32. a first hydrophobic layer; 33. a second hydrophobic layer; 4. a control layer; 40. a photoelectric sensing device; 41. a first switching device; 410. a first source electrode; 411. a first drain electrode; 412. a first active layer; 413. a first gate insulating layer; 414. a first gate electrode; 42. a drive electrode; 43. a second switching device; 430. a second source electrode; 431. a second drain electrode; 432. a second active layer; 433. a second gate insulating layer; 434. a second gate electrode; 5. a first transparent substrate; 6. a second transparent substrate; 7. a transparent conductive layer; 8. a temperature detection zone; 9. a sample entry zone; 10. a reagent sample introduction zone; 11. a concentration detection zone; 12. a sample reagent mixing zone; 13. a sample detection zone; 14. a collection region; 15. a first region; 16. a second region; 17. a light source.
Detailed Description
Example embodiments will now be described more fully with reference to the accompanying drawings. Example embodiments may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The same reference numerals in the drawings denote the same or similar structures, and thus their detailed description will be omitted.
Although relative terms, such as "upper" and "lower," may be used in this specification to describe one element of an icon relative to another, these terms are used in this specification for convenience only, e.g., in accordance with the orientation of the examples described in the figures. It will be appreciated that if the device of the icon were turned upside down, the element described as "upper" would become the element "lower". When a structure is "on" another structure, it may mean that the structure is integrally formed with the other structure, or that the structure is "directly" disposed on the other structure, or that the structure is "indirectly" disposed on the other structure via another structure.
In the drawings, the thickness of regions and layers may be exaggerated for clarity. The same reference numerals denote the same or similar structures in the drawings, and thus detailed descriptions thereof will be omitted.
The terms "a," "an," "the," "said" are used to indicate the presence of one or more elements/components/etc.; the terms "comprising" and "having" are intended to be inclusive and mean that there may be additional elements/components/etc. other than the listed elements/components/etc.; the terms "first" and "second" are used merely as labels, and are not limiting on the number of their objects.
In the related art, the temperature measurement method of the micro-fluidic chip is to set a thermocouple at one side of the micro-fluidic chip to measure the temperature of the sample 100 to be detected in the temperature detection area, and the thermocouple detects the temperature in a heat conduction manner, so that the temperature measurement method has hysteresis, the measurement error is large, and the operation is complicated because the thermocouple needs to be specially installed during detection.
As shown in fig. 1, the present disclosure provides a microfluidic chip, which can be used to detect temperature values of a sample to be tested 100 in biological, chemical and medical analysis processes. For example, the sample 100 to be tested may be cells, proteins, chromosomes, etc. in a solution in a biological tissue. Of course, the sample 100 to be tested may also be other liquid substances, which are not listed here.
The microfluidic chip of the embodiment of the present disclosure includes a substrate 1, a thermochromatic liquid crystal layer 2, a flow channel layer 3, and a control layer 4. Wherein,
the thermochromatic liquid crystal layer 2 is arranged opposite to the substrate 1;
the flow channel layer 3 is arranged on one side of the thermochromatic liquid crystal layer 2 close to the substrate 1 and is provided with a flow channel 30 for the sample 100 to be detected to flow;
the control layer 4 is arranged between the substrate 1 and the flow channel layer 3, the control layer 4 comprises at least one control unit, the at least one control unit is a target control unit, and the target control unit comprises a photoelectric sensing device 40; the photo sensor device 40 is used for receiving light passing through the thermochromatic liquid crystal layer 2 and the flow channel layer 3 and generating an electrical signal reflecting temperature according to the intensity of the light.
When the micro-fluidic chip system and the micro-fluidic chip provided by the disclosure are used, a sample to be detected 100 is introduced into the flow channel 30 of the flow channel layer 3 of the micro-fluidic chip, and light is emitted to the thermochromatic liquid crystal layer 2 through the light source 17; the temperature of the sample 100 to be detected affects the color of the thermochromatic liquid crystal layer 2, so that the light transmittance of the thermochromatic liquid crystal layer 2 is changed, and the intensity of transmitted light after the light passes through the thermochromatic liquid crystal layer 2 and the flow channel layer 3 is further affected; the photoelectric sensing device 40 receives the transmitted light and generates a corresponding electric signal according to the intensity of the transmitted light; the electric signal is analyzed to obtain the temperature value of the sample 100 to be detected. The micro-fluidic chip can measure the temperature of a sample to be detected 100, and the measurement error is small.
The components of the microfluidic chip provided by the embodiments of the present disclosure are described in detail below with reference to the accompanying drawings:
as shown in fig. 1, the shape of the substrate 1 may be rectangular, circular or other shapes, which are not listed here.
As shown in fig. 1, the thermochromatic liquid crystal layer 2 may include thermochromatic liquid crystals, and when the temperature of the thermochromatic liquid crystals changes, the transmittance of the thermochromatic liquid crystals changes, and the intensity of transmitted light passing through the thermochromatic liquid crystal layer 2 changes. The thermochromic liquid crystal layer 2 may be arranged opposite the substrate 1, the projection of the thermochromic liquid crystal layer 2 onto the substrate 1 being located within the edges of the substrate 1.
In order to facilitate mounting the thermochromic liquid crystal layer 2, in one embodiment, the microfluidic chip according to the embodiments of the present disclosure may further include a first transparent substrate 5 and a second transparent substrate 6, and a cavity may be formed between the second transparent substrate 6 and the first transparent substrate 5, and the cavity is used to accommodate the thermochromic liquid crystal layer 2. Wherein:
the first transparent substrate 5 may be transparent glass or transparent ceramic, or may be other transparent materials, which are not listed here. The second transparent substrate 6 may be a cured transparent potting adhesive, and has excellent properties such as moisture resistance and insulation. Of course, the second transparent substrate 6 may be transparent glass, transparent ceramic, or the like, which is not listed here. The thicknesses of the first transparent substrate 5 and the second transparent substrate 6 are not particularly limited herein.
The cavity is used as a containing space of the thermochromatic liquid crystal layer 2, a groove can be formed in the surface, close to the substrate 1, of the first transparent substrate 5, or a groove can be formed in the surface, far away from the substrate 1, of the second transparent substrate 6, or grooves can be formed in the surfaces, adjacent to the first transparent substrate 5 and the second transparent substrate 6, of the first transparent substrate 5 and the second transparent substrate 6 are attached to form the cavity. The depth of the grooves may be greater than or equal to the thickness of the thermochromic liquid crystal layer 2 so that the grooves can accommodate the thermochromic liquid crystal layer 2. It should be noted that the cavities may be either coherent or incoherent. When the cavities are not connected, the workload of opening the cavities can be reduced, the thermochromatic liquid crystal layer 2 can be saved, and the cost of the microfluidic chip is finally reduced.
As shown in fig. 1, the flow channel layer 3 may have a flow channel 30, and the sample to be inspected 100 can flow along the flow channel 30.
The flow channel 30 has an inlet and an outlet, and the sample 100 to be detected enters the microfluidic chip through the inlet and is discharged out of the microfluidic chip through the outlet. The shape of the inlet and outlet may be square or circular, etc., and the specific size of the inlet and outlet is not particularly limited herein. In addition, the positions of the inlet and outlet are subject to convenient arrangement and will not be described in detail herein.
In order to prevent the sample 100 to be tested from leaking, in one embodiment, the flow channel layer 3 of the embodiment of the present disclosure may include a first hydrophobic layer 32, a second hydrophobic layer 33, and a frame 31, and the three can enclose the flow channel 30, wherein:
the first hydrophobic layer 32 and the second hydrophobic layer 33 can be disposed between the control layer 4 and the thermochromatic liquid crystal layer 2, and the two layers are disposed opposite to each other, so that the sample 100 to be detected can be disposed between the two layers. For example, the material of the first hydrophobic layer 32 and the second hydrophobic layer 33 may be teflon or the like, which is not listed here. The first water-repellent layer 32 and the second water-repellent layer 33 may be formed by spin coating or the like, and will not be described in detail here.
The frame 31 may be disposed between the first hydrophobic layer 32 and the second hydrophobic layer 33, and forms a flow channel 30 with the first hydrophobic layer 32 and the second hydrophobic layer 33 for the sample 100 to be tested to flow. For example, the frame 31 may be rectangular, circular or other shapes, and may be formed by connecting a plurality of frame strips end to end, and disposed on the outer edge of the microfluidic chip, where the frame strips may be connected by bonding and clamping, or may be an integrated structure. Meanwhile, a plurality of support columns may be disposed in the flow channel 30 to support the first and second water-repellent layers 23 and 33 to prevent collapse.
In another embodiment, the flow channel layer 3 may not include the first hydrophobic layer 32 and the second hydrophobic layer 33, the frame 31 may be disposed between the second transparent substrate 6 and the control layer 4, and the frame 31, the second transparent substrate 6 and the control layer 4 may also enclose the flow channel 30 for the sample 100 to be tested to flow.
It should be noted that, in order to reduce the resistance of the sample 100 to be detected flowing through the flow channel 30, a lubricating layer may be formed on the inner wall of the flow channel 30, and the material of the lubricating layer may be solid mineral oil, paraffin wax or other lubricating materials, which not only can reduce the power of the driving electrode 42 for driving the sample 100 to be detected to flow, but also can increase the detection speed of the temperature of the sample 100 to be detected.
As shown in fig. 1, the control layer 4 may be disposed between the substrate 1 and the flow channel layer 3, the control layer 4 may include control units, the number of the control units may be one, two, three or more, and at least one of the control units is a target control unit.
The target control unit may include a photo sensor device 40, the photo sensor device 40 may be a PIN photodiode or other device capable of performing photoelectric conversion, the photo sensor device 40 corresponds to the thermochromatic liquid crystal layer 2, and is configured to receive the transmitted light passing through the thermochromatic liquid crystal layer 2 and the flow channel layer 3, and generate an electrical signal capable of reflecting the temperature of the sample 100 to be detected according to the intensity of the transmitted light, and the electrical signal varies with the intensity of the light, so that the temperature value of the sample 100 to be detected may be determined according to the electrical signal.
The target control unit may further include a first switching device 41, and the first switching device 41 is connected to the photo sensing device 40 for turning on or off the photo sensing device 40.
For example, as shown in fig. 1, the first switching device 41 may be a Thin Film Transistor (TFT), the thin film transistor may be a bottom gate thin film transistor, and may include a first source 410, a first drain 411, a first active layer 412, a first gate insulating layer 413 and a first gate 414, the first gate 414 is disposed on a surface of the substrate 1 close to the thermochromatic liquid crystal layer 2, the first gate insulating layer 413 covers the first gate 414, the first active layer 412 is disposed on a surface of the first gate insulating layer 413 away from the substrate 1, the first source 410 and the first drain 411 are disposed on a surface of the first active layer 412 away from the first gate insulating layer 413, and the first source 410 is connected to the photo-sensing device 40 for controlling the photo-sensing device 40 to be powered on or powered off.
Of course, the first switching device 41 may also be a top gate thin film transistor or other switching devices, which are not listed here.
In order to determine the temperature value of the sample 100 to be detected according to the electrical signal, the micro-fluidic chip of the embodiment of the present disclosure may include a processor, and the processor is connected to the photoelectric sensing device 40, and is configured to receive the electrical signal sent by the photoelectric sensing device 40, and determine the temperature value of the sample 100 to be detected according to a preset correspondence between the electrical signal and the temperature.
As shown in fig. 2, the microfluidic chip according to the embodiment of the present disclosure may include a temperature detection region 8, the number of target control units is plural, and the target control units may be distributed in the temperature detection region 8 in an array, and an orthographic projection of the thermochromatic liquid crystal layer on the substrate 1 is located in the temperature detection region 8. The temperature detection zone 8 may comprise a plurality of sub-zones distributed in an array, and each sub-zone may be the same size. For example, the number of sub-regions in the temperature detection region 8 may be 16, as shown in fig. 4, and the sub-regions are distributed in a rectangular array as a first row, a second row, a third row and a fourth row, wherein the sub-regions in the first row may be sequentially labeled as a 1-a 4, the sub-regions in the second row may be sequentially labeled as B1-B4, the sub-regions in the third row may be sequentially labeled as C1-C4, the sub-regions in the fourth row may be sequentially labeled as D1-D4, and each sub-region is provided with a target control unit. When the sample to be inspected 100 exists in any one of the sub-areas, the temperature of the sample to be inspected 100 can be detected by the target control unit in the sub-area.
The microfluidic chip of the embodiment of the present disclosure may further include a transparent conductive layer 7, each control unit further includes a driving unit, that is, each target control unit also includes a driving unit, and the driving unit and the transparent conductive layer 7 realize the movement of the sample 100 to be detected based on the dielectric wetting principle, specifically:
the transparent conductive layer 7 can be disposed between the second transparent substrate 6 and the first hydrophobic layer 32, and the material of the transparent conductive layer 7 can be a transparent conductive material such as ITO (indium tin oxide), which is not listed here.
Each driving unit may include a driving electrode 42 and a second switching device 43. Wherein, the driving electrode 42 is arranged opposite to the transparent conductive layer 7, after the driving electrode 42 is electrified, an electric field is formed between the driving electrode 42 and the transparent conductive layer 7, the contact angle of the local part of the droplet-shaped sample 100 to be detected is reduced, so that the local shape of the droplet-shaped sample 100 to be detected is changed, and the internal pressure difference is caused; by sequentially energizing the driving electrodes 42 of two adjacent driving units, the droplet-shaped sample to be inspected 100 is moved between positions corresponding to the two adjacent driving electrodes 42 under the driving of the pressure difference. When the number of the driving units is plural and the driving units are arranged along a predetermined path, the droplet-shaped sample to be tested 100 can be moved along the path formed by the driving units by sequentially energizing the driving electrodes 42 of the respective driving units.
The second switching devices 43 may be connected to the driving electrodes 42 for energizing or de-energizing the driving electrodes 42 to turn on or off the driving electrodes 42, and the second switching devices 43 may be independently controlled to control the driving electrodes 42 of the respective driving units to be energized/de-energized in a preset sequence, respectively.
For example, as shown in fig. 1, the second switching device 43 may be a Thin Film Transistor (TFT), which may be a bottom gate thin film transistor, and may include a second source electrode 430, a second drain electrode 431, a second active layer 432, a second gate insulating layer 433, and a second gate electrode 434, wherein the second gate electrode 434 is disposed on the surface of the substrate 1 close to the thermochromic liquid crystal layer 2, the second gate insulating layer 433 covers the second gate electrode 434, the second active layer 432 is disposed on the surface of the second gate insulating layer 433 away from the substrate 1, the second source electrode 430 and the second drain electrode 431 are disposed on the surface of the second active layer 432 away from the second gate insulating layer 433, and the second drain electrode 431 is connected to the driving electrode 42 for controlling the driving electrode 42 to be powered on or powered off.
Of course, the second switching device 43 may also be a top gate thin film transistor or other switching device, which is not listed here.
For example, when the sample to be detected 100 is a droplet with a small volume in the temperature detection region 8, the orthographic projection of a single droplet on the control layer 4 can be slightly larger than the area of a single control unit, as shown in fig. 4. When the temperature detection zone 8 is provided with three droplets, as shown in fig. 5, the second switching device 43 of the control unit in the sub-zone of C1 can be controlled to open the corresponding drive electrode 42, pushing the droplets from the sub-zone of C1 to the sub-zone of C2. At this time, the driving electrodes 42 in the control units of the sub-areas B2, C2 and C3 are controlled to move to push the three small drops to move towards each other, and the three small drops are merged into a large drop, as shown in FIG. 6. The first switch devices 41 in the B2 sub-area control unit, the C2 sub-area control unit, the B3 sub-area control unit and the C3 sub-area control unit are controlled to open the corresponding photoelectric sensing devices 40, so that large liquid drops can be subjected to temperature measurement. To sum up, the micro-fluidic chip can realize the detection of the sample to be detected 100 at any position of the temperature detection area 8, a plurality of samples and different volumes, and the detection flexibility and efficiency of the micro-fluidic chip are enhanced.
As shown in fig. 2, the microfluidic chip may further include a sample introduction region 9, a reagent introduction region 10, a concentration detection region 11, a sample reagent mixing region 12, a sample detection region 13, and a collection region 14. Wherein, the sample feeding area 9 and the reagent feeding area 10 are arranged at the same side of the temperature detection area 8; the concentration detection zone 11 may be disposed on a side of the temperature detection zone 8 away from the sample introduction zone 9; the sample reagent mixing zone 12 may be disposed on a side of the temperature detection zone 8 away from the concentration detection zone 11; the sample detection zone 13 may be disposed on a side of the sample reagent mixing zone 12 away from the temperature detection zone 8; the collection zone 14 may be provided on the side of the sample reagent mixing zone 12 remote from the sample detection zone 13.
Sample advances appearance zone 9, reagent advances appearance zone 10, concentration detection zone 11, sample reagent mixes district 12, sample detection zone 13 all includes a plurality of the control unit that are array distribution, drive unit's second switching element 43 opens corresponding drive electrode 42 in controlling each control unit according to predetermined order, can make and wait to examine sample 100 and move between concentration detection zone 11, sample reagent mixes district 12, sample detection zone 13, the removal of waiting to examine the sample can refer to temperature detection 8 in specific removal process, no longer redundance here.
The sample feeding area 9 provides a channel for a sample 100 to be detected to enter the microfluidic chip, and the reagent feeding area 10 provides a channel for a reagent to enter the microfluidic chip. The reagent may be a catalyst for optimizing the preparation, reaction, separation and detection processes of the sample to be tested, and will not be described in detail herein.
The concentration detection zone 11 may be divided into two parts, one part being connected to the sample introduction zone 9 for detecting the concentration of the sample 100 to be tested, and the other part being connected to the reagent introduction zone 10 for detecting the concentration of the reagent. The movement of the sample 100 to be detected in the concentration detection area 11 can be realized by the driving unit and the transparent conductive layer 7, and the specific principle can refer to the movement process of the sample 100 to be detected in the temperature detection area, which is not described herein in detail.
The number of the target control units of the concentration detection zone 11 is plural, and may be distributed in an array within the concentration detection zone 11, as shown in fig. 2. Since the concentration of the sample to be inspected 100 affects the intensity of the transmitted light, the concentration of the sample can be detected by the target control unit, and the structure of the target control unit can be identical to that of the temperature detection region, which will not be described in detail herein. Meanwhile, in order to avoid the influence of the thermochromic liquid crystal layer 2 on the concentration detection, the thermochromic liquid crystal layer 2 does not need to be provided in the concentration detection region 11. During detection, in the concentration detection area 11, light rays sequentially pass through the first transparent substrate 5, the second transparent substrate 6 and the flow channel layer 3, the photoelectric sensing device 40 receives penetrating light, an electric signal reflecting concentration is generated according to the intensity of the penetrating light, the electric signal changes along with the change of the intensity of the light, and therefore the concentration value of the sample 100 to be detected can be determined according to the electric signal.
It should be noted that, since the concentration of the sample to be tested 100 affects the intensity of the penetrating light received by the photoelectric sensing device 40, there is a certain deviation between the detection temperature and the actual temperature of the sample to be tested 100 detected by the micro-fluidic chip. Therefore, the actual temperature of the samples 100 to be detected with a plurality of preset concentrations can be detected in advance through experiments, the detection temperature of the samples 100 to be detected with a plurality of preset concentrations can be obtained through the microfluidic chip, and the deviation value between the actual temperature and the detection temperature under each preset concentration can be determined according to each actual temperature and the detection temperature. In the using process of the micro-fluidic chip, the concentration of the sample 100 to be detected is detected through the concentration detection area 11, the temperature is detected through the temperature detection area 8, the detection temperature is compensated by combining the deviation value, and the actual temperature of the sample 100 to be detected is output, so that the temperature measurement of the micro-fluidic chip is more accurate.
For example, the processor may obtain the temperature detected by the temperature detection area 8 and the concentration of the sample 100 to be detected by the concentration detection area 11, determine an offset value corresponding to the concentration, compensate the temperature value according to the offset value, and output the actual temperature of the sample 100 to be detected.
Sample reagent mixing zone 12 is used to effect mixing of reagents and sample 100 to be tested. The respective driving units of the sample reagent mixing area 12 drive the reagent and the sample to be tested 100 to move towards each other and realize the mixing of the two. The movement of the reagent and the sample 100 to be detected can be realized by the driving unit and the transparent conductive layer 7, and the specific principle can refer to the movement process of the sample 100 to be detected in the temperature detection area, which is not described herein in detail.
The sample detection area 13 is used for detecting the sample 100 to be detected, a detection device for detecting the sample 100 to be detected can be arranged in the sample detection area 13, and the type of the detection device can be selected according to the parameters to be detected. For example, the detection device may be a PH detection device or a detection device related to an enzyme-linked reaction, and the detection device is disposed at a corresponding position, which is not described in detail herein.
The collection region 14 provides a path for the sample 100 to be tested to exit the microfluidic chip and will not be described in detail herein.
The microfluidic chip of the embodiment of the present disclosure may further include a first area 15 and a second area 16, where the first area 15 and the second area 16 may both have a plurality of control units distributed in an array and correspond to the transparent conductive layer 7; the first region 15 connects the concentration detection zone 11 and the collection zone 14, and the second region 16 connects the concentration detection zone 11 and the collection zone 14.
The control units in the first area 15 and the second area 16 can drive the sample 100 to be detected to move along the first area 15 and the second area 16 through the driving units, and the specific principle can refer to the moving process of the sample 100 to be detected in the temperature detection area, which is not described herein again. Thus, the sample 100 to be tested can directly move to the collection area 14 via the first area 15 without passing through the transition detection area 8, the sample reagent mixing area 12 and the sample detection area 13, and then can be discharged from the microfluidic chip. The reagent can directly move to the collecting area 14 through the second area 16 without sequentially passing through the transition detection area 8, the sample reagent mixing area 12 and the sample detection area 13, and then is discharged out of the microfluidic chip.
The embodiment of the present disclosure further provides a micro-fluidic chip system, which may include a light source 17 and the micro-fluidic chip of any of the above embodiments, where the light source 17 is disposed on a side of the thermochromatic liquid crystal layer 2 away from the flow channel layer 3, and is configured to emit light to the thermochromatic liquid crystal layer 2.
For example, the light source 17 may be an LED or a laser fixed on the worktable, which is not listed here. The wavelength of the light emitted by the light source 17 may be selected according to the material of the thermochromic liquid crystal layer 2. In addition, an optical filter may be disposed between the light source 17 and the microfluidic chip to filter out light with wavelengths other than the specific wavelength, so that only light with the specific wavelength is irradiated to the microfluidic chip.
As shown in fig. 3, the light impinges on the thermochromic liquid crystal layer 2 at an angle of incidence α, which may be zero, i.e. the light impinges perpendicularly. Of course, α may be an acute angle, and the light irradiates the thermochromic liquid crystal layer 2 and is divided into two parts of reflected light and refracted light, which passes through the thermochromic liquid crystal layer 2 and the flow channel layer 3 and is received by the photo-sensor device 40.
Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure disclosed herein. This application is intended to cover any variations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
Claims (9)
1. A microfluidic chip, comprising:
a substrate;
a thermochromatic liquid crystal layer disposed opposite to the substrate;
the flow channel layer is arranged on one side of the thermochromatic liquid crystal layer close to the substrate and is provided with a flow channel for flowing of a sample to be detected;
the first transparent substrate is arranged on the surface of the thermochromatic liquid crystal layer far away from the substrate;
the second transparent substrate is arranged on the surface, close to the substrate, of the thermochromatic liquid crystal layer, and a cavity corresponding to the target control unit is formed between the second transparent substrate and the first transparent substrate;
the transparent conducting layer is arranged between the second transparent substrate and the flow channel layer;
the control layer is arranged between the substrate and the flow channel layer and comprises at least two control units, at least one control unit is the target control unit, and the target control unit comprises a photoelectric sensing device; the photoelectric sensing device is used for receiving light rays passing through the thermochromatic liquid crystal layer and the flow channel layer and generating an electric signal capable of reflecting the temperature of the sample to be detected according to the intensity of the light rays;
each control unit comprises a driving unit, each driving unit comprises a driving electrode and a second switch device, and the driving electrode is arranged opposite to the transparent conducting layer and used for forming an electric field for driving the sample to be detected to move with the transparent conducting layer; the second switching device is used to turn on/off the driving electrode.
2. The microfluidic chip according to claim 1, wherein the photo-sensing device is a photodiode.
3. The microfluidic chip according to claim 1, wherein the target control unit further comprises:
and the first switching device is connected with the photoelectric sensing device and used for switching on or switching off the photoelectric sensing device.
4. The microfluidic chip according to claim 3, wherein the first switching device is a thin film transistor.
5. The microfluidic chip according to claim 1, wherein the thermochromatic liquid crystal layer is defined in the cavity and is directly opposite to the target control unit.
6. The microfluidic chip according to claim 5, wherein the channel layer comprises:
the first hydrophobic layer is arranged between the control layer and the thermochromatic liquid crystal layer;
the second hydrophobic layer is arranged between the control layer and the thermochromatic liquid crystal layer and is opposite to the first hydrophobic layer;
the frame is arranged between the first hydrophobic layer and the second hydrophobic layer, and the flow channel is a space surrounded by the first hydrophobic layer, the second hydrophobic layer and the frame.
7. The microfluidic chip according to claim 1, wherein the control layer includes a temperature detection area, the target control units are plural in number, and the array is distributed in the temperature detection area.
8. The microfluidic chip according to claim 7, wherein an orthographic projection outer edge of the thermochromatic liquid crystal layer on the control layer is located within an outer edge of the temperature detection region.
9. A microfluidic chip system, comprising:
a microfluidic chip according to any one of claims 1 to 8;
the light source is arranged on one side, away from the flow channel layer, of the thermochromatic liquid crystal layer and used for emitting light to the thermochromatic liquid crystal layer.
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| WO2020210981A1 (en) * | 2019-04-16 | 2020-10-22 | Boe Technology Group Co., Ltd. | Micro-channel device and manufacturing method thereof and micro-fluidic system |
| CN112138729A (en) * | 2019-06-26 | 2020-12-29 | 京东方科技集团股份有限公司 | Sampling structure, seal structure and detection subassembly |
| CN112175815B (en) * | 2019-07-05 | 2023-04-11 | 京东方科技集团股份有限公司 | PCR substrate, chip, system and droplet drawing method |
| CN110597328B (en) * | 2019-09-18 | 2021-04-23 | 重庆大学 | Flow cooperative control system based on liquid crystal temperature control micro valve |
| CN110523450B (en) * | 2019-09-30 | 2022-01-25 | 京东方科技集团股份有限公司 | Microfluidic substrate, microfluidic chip, microfluidic system and detection method |
| CN114632557B (en) * | 2020-12-16 | 2024-05-28 | 合肥京东方光电科技有限公司 | Opposite substrate of micro-fluidic chip and micro-fluidic chip |
| CN116351351A (en) * | 2021-12-28 | 2023-06-30 | 彩科(苏州)生物科技有限公司 | Transistor optical tweezers with increased illuminated intensity and microfluidic device |
| CN116393068A (en) * | 2021-12-28 | 2023-07-07 | 彩科(苏州)生物科技有限公司 | Optical tweezers device and micro-fluid device based on oblique equi-density arrangement of phototransistors |
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| GB0412981D0 (en) * | 2004-06-10 | 2004-07-14 | Rue De Int Ltd | Improvements in security devices |
| US20070132898A1 (en) * | 2005-11-04 | 2007-06-14 | Union College | Light transmission based liquid crystal temperature sensor |
| US9063091B2 (en) * | 2012-04-06 | 2015-06-23 | Ixensor Inc. | Test strips and method for reading test strips |
| CN105510794B (en) * | 2016-01-11 | 2018-05-25 | 中国电子科技集团公司第十研究所 | High electron mobility transistor PHEMT thermo-resistance measurement methods |
| WO2017152357A1 (en) * | 2016-03-08 | 2017-09-14 | Coyote Bioscience Co., Ltd. | Methods and systems for analyzing nucleic acids |
| CN105823575B (en) * | 2016-05-05 | 2018-02-13 | 北京航空航天大学 | The scaling method and thermometry of Pixel-level thermochromic liquid crystal |
| CN106706155A (en) * | 2016-11-30 | 2017-05-24 | 维沃移动通信有限公司 | Temperature detection method and device |
| CN107588865A (en) * | 2017-10-23 | 2018-01-16 | 盐城工学院 | Temperature collection circuit and Optical Receivers |
| CN108760644B (en) * | 2018-05-25 | 2021-01-26 | 京东方科技集团股份有限公司 | Method and system for monitoring liquid |
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