CN205426923U - Elisa plate - Google Patents
Elisa plate Download PDFInfo
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- CN205426923U CN205426923U CN201620233863.8U CN201620233863U CN205426923U CN 205426923 U CN205426923 U CN 205426923U CN 201620233863 U CN201620233863 U CN 201620233863U CN 205426923 U CN205426923 U CN 205426923U
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- Prior art keywords
- elisa plate
- plate hole
- funnel
- hemisphere
- shaped structure
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- 210000003141 lower extremity Anatomy 0.000 claims description 7
- 238000002965 ELISA Methods 0.000 claims 7
- 238000001514 detection method Methods 0.000 abstract description 9
- 239000007790 solid phase Substances 0.000 abstract description 8
- 238000012864 cross contamination Methods 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000013077 target material Substances 0.000 abstract 1
- 239000011148 porous material Substances 0.000 description 7
- 239000013076 target substance Substances 0.000 description 7
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 241001141491 Eumorpha elisa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an elisa plate, a serial communication port, including a plurality of elisa plate holes, the elisa plate hole include three component parts: every elisa plate hole include hemispherical bottom, with hemispherical bottom meet set up in the funnel -shaped structure at middle part and with funnel -shaped structure meets and sets up the salient wall in upper portion, the hemispherical polycrystalline substance of elisa plate can increase elisa plate's surface area to target material and the chance that the surperficial target molecule of solid phase contacted is increased, the content of less sample volume target detection material can be used simultaneously, elisa plate funnel -shaped structure set up bottom and the upper portion of connecting the elisa plate hole, further increase the surface area in elisa plate hole, the setting of salient wall can prevent cross contamination between elisa plate hole and the elisa plate hole.
Description
Technical field
This utility model relates to immunoassay device field, particularly relates to a kind of ELISA Plate.
Background technology
Immune detection is the method for life science modal detection target molecule, and elisa (ELISA) is the important component part of immune detection.ELISA is broadly divided into two kinds of reactions: a kind of is the immunoreation between antigen and antibody, and another kind is biomolecule and the solid phase surface adsorbed.In elisa (ELISA), participate in the antigen of immunological response, antibody, traget antibody or the purity of antigen, concentration and ratio；Buffer kind, concentration and ionic strength, pH value and the condition such as reaction temperature, time play pivotal role.Additionally, the ELISA Plate surface as carrier also plays very important effect to the absorption of antigen, antibody or antigen antibody complex.
In prior art, the most frequently used material of ELISA ELISA Plate is polystyrene.In recent years, in order to increase the affinity of biomolecule and solid phase surface, improve the sensitivity of reaction, strengthening the stability etc. of detection, the selection of ELISA Plate solid phase material and process cause the greatly concern of this area research worker.At present, this area research worker is devoted to study the chemical property being modified the method such as polystyrene surface and ultraviolet irradiation change polystyrene surface by covalent cross-linking chemical group activating functional groups, chemical reaction more, and then improve the ELISA Plate hole surface affinity to biomolecule, and have been achieved for significant progress.
But, polystyrene ELISA Plate still suffers from another problem Surface area considerations that i.e. ELISA Plate hole and biomolecule are reacted.ELISA Plate majority of the prior art is at the bottom of flat (structure such as Figure of description 1), the U-shaped end or V-type, flat refractive index is low, be suitable to microplate reader detection, the ELISA Plate refractive index at the U-shaped end is higher, convenient sample-adding, inhaling the operation such as sample, mixing, the ELISA Plate at the bottom of V can pipette samples accurately.But the ELISA Plate hole of above-mentioned several ELISA Plate is less with the surface area that biomolecule is reacted, it is unfavorable for fully quickly carrying out of reaction.
Utility model content
In view of this, the purpose of this utility model is to provide the ELISA Plate that a kind of surface area is big, applied sample amount is little.To achieve these goals, this utility model offer techniques below scheme:
This utility model provides a kind of ELISA Plate, it is characterised in that include that some ELISA Plate holes, each ELISA Plate hole include the funnel-shaped structure that hemispherical bottom connects and the protrusion wall connected with described funnel-shaped structure top edge with described hemispherical bottom top edge.
Preferably, described hemispherical bottom includes primary hemisphere structure and is positioned at the inwardly protruded secondary hemisphere structure of primary hemisphere inside configuration.
Preferably, a diameter of the 0.2～10mm of described primary hemisphere structure, wall thickness is 0.1～1.0mm.
Preferably, the hemispheroidal number of secondary of each primary hemisphere inside configuration is 3～50, and described secondary hemisphere structure is evenly distributed in the inner surface of primary hemisphere structure.
Preferably, a diameter of the 0.1～5mm of described secondary hemisphere structure, wall thickness is 0.1～1.0mm, and the interstructural hole of neighboring secondary hemisphere is 0.1～1.0mm.
Preferably, described funnel-shaped structure is made up of the hollow segment and the hollow round table two parts connected with described hollow segment top edge being positioned at bottom, and the lower limb of described hollow segment shape connects with the top edge of described hemispherical bottom；The top edge of described hollow round table is connected with the lower limb of described protrusion wall.
Preferably, a diameter of the 0.2～10mm of described hollow segment, height is 0.1～5.0mm, and wall thickness is 0.1～1.0mm.
Preferably, the height of described hollow round table is 0.2～6.0mm, and wall thickness is 0.1～1.0mm, and upper port radius is 0.1～5.0mm, and lower port radius is 0.1～5.0mm..
Preferably, described protrusion wall is hollow column structure, and the height of described protrusion wall is 0.1～20mm, and wall thickness is 0.1～1.0mm, and radius is 0.1～5.0mm.
Preferably, the area of ELISA Plate is 10～200 × 5～200mm ELISA Plate, and each ELISA Plate preferably includes 1～500 ELISA Plate hole.
Beneficial effect:
The ELISA Plate that this utility model provides includes that some ELISA Plate holes, each ELISA Plate hole include the funnel-shaped structure that hemispherical bottom connects and the protrusion wall connected with described funnel-shaped structure top edge with described hemispherical bottom top edge.In the ELISA Plate that this utility model provides, described hemispherical polycrystalline substance can increase the surface area of ELISA Plate, thus increases the chance that target substance contacts with solid phase surface target molecule, simultaneously can be with the content of less sample size detection target substance；The bottom arranging ligase target hole of described funnel-shaped structure and top, increase the surface area in ELISA Plate hole further；The described setting protruding wall can prevent cross-contamination between ELISA Plate hole and ELISA Plate hole.
Accompanying drawing explanation
Fig. 1 is the axonometric chart in ELISA Plate hole disclosed in prior art；
Fig. 2 is top view (2A) and the front view (2B) of the ELISA Plate pore structure in this utility model embodiment 1；
Fig. 3 is the sectional view of the ELISA Plate pore structure in embodiment 1；
Fig. 4 is the axonometric chart of the ELISA Plate pore structure in embodiment 1；
Fig. 5 is top view (5A-1,5B-1) and the side view (5A-2,5B-2) of 20 holes and 28 hole ELISA Plate in embodiment 1；
Fig. 6 is the sectional view (6A, 6B) of 20 holes and 28 hole ELISA Plate in embodiment 1 and 2；
Fig. 7 is the axonometric chart (7A, 7B) of ELISA Plate in embodiment 1 and 2；
Detailed description of the invention
This utility model provides a kind of ELISA Plate, including some ELISA Plate holes, described ELISA Plate hole includes three ingredients: each ELISA Plate hole includes that hemispherical bottom and described hemispherical bottom connection are arranged at the funnel-shaped structure at middle part and connect the protrusion wall being placed in top with described funnel-shaped structure.
In this utility model, the area to described ELISA Plate is 10～200 × 5～200mm ELISA Plate, and each ELISA Plate preferably includes 1～500 ELISA Plate hole, preferred includes 10～400 ELISA Plate holes, most preferably includes 16 or 40 ELISA Plate holes.In described ELISA Plate, the density in ELISA Plate hole is preferably 0.2～8.0/cm2, more preferably 0.5～4/cm2.This utility model does not has special restriction to the arrangement mode in described ELISA Plate hole, uses the mode of ELISA Plate hole arrangement in ELISA Plate well known to those skilled in the art；In embodiment of the present utility model, described ELISA Plate hole preferably uses rectangular array, more preferably uses 20 hole ELISA Plate of 4 × 5 rectangular array or 28 hole ELISA Plate of 4 × 7 rectangular array.
In this utility model, the structure in ELISA Plate hole is as shown in Figure 3, each ELISA Plate hole includes hemispherical bottom, bottom is set to hemispherical configuration and adds the surface area of ELISA Plate, thus increase the chance that target substance contacts with solid phase surface target molecule, simultaneously can be with the content of less sample size detection target substance.In this utility model, described hemispherical bottom preferably includes primary hemisphere structure (2) and is positioned at the inwardly protruded secondary hemisphere structure (3) of primary hemisphere inside configuration.The diameter of the primary hemisphere structure described in this utility model is preferably 0.2～10mm, more preferably 2～8mm, most preferably 6-7mm.
This utility model preferably arranges inwardly protruded secondary hemisphere structure in each primary hemisphere inside configuration, and in each primary hemisphere structure, secondary hemispheroidal number is preferably 3～50, more preferably 5～15, most preferably 7～9；Described in this utility model, secondary hemisphere structure is evenly distributed in the inner surface of primary hemisphere structure.
The diameter of the secondary hemisphere structure described in this utility model is preferably 0.1～5mm, more preferably 0.5～3mm, most preferably 1～2mm.Being preferably 0.1～2.0mm in the interstructural arc length of neighboring secondary hemisphere described in this utility model, in this utility model, the interstructural hole of neighboring secondary hemisphere is preferably 0.1～1.0mm, more preferably 0.4～0.6mm, most preferably 0.5mm.In this utility model, the described interstructural hole of neighboring secondary hemisphere is the liquid flow path in ELISA Plate hole, thus allows liquid to flow freely inside ELISA Plate hole, increases the chance that target substance contacts with solid phase surface target molecule.
In this utility model, each ELISA Plate hole includes the funnel-shaped structure (4) connected with described hemispherical bottom top edge, described funnel shaped structure is arranged at the middle part in ELISA Plate hole, and upwards the lower limb with described protrusion wall (7) is connected.In this utility model, described funnel-shaped structure is made up of the hollow segment structure (5) of funnel-shaped structure bottom and hollow round table (6) two parts on funnel-shaped structure top；The described lower limb of hollow segment shape structure is connected with the top edge of the hemispherical bottom bottom ELISA Plate hole；The top edge of described hollow round table is connected with the lower limb of described protrusion wall.
In this utility model, the diameter of described hollow segment is preferably 0.2～10mm, more preferably 3～8mm, most preferably 4.8mm；The highly preferred of described hollow segment is 0.1～5.0mm, more preferably 0.5～3mm, most preferably 0.8mm；The wall thickness of described hollow segment is preferably 0.1～1.0mm, more preferably 0.3～0.8mm, most preferably 0.7mm
In this utility model, the highly preferred of the hollow round table on funnel-shaped structure top is 0.2～mm, preferred 0.3～3mm, most preferably 0.4mm；The wall thickness of described hollow round table is preferably 0.1～1.0mm, more preferably 0.2～0.8mm, most preferably 0.7mm；The upper port radius of described hollow round table is preferably 0.1～5.0mm, more preferably 1～4mm；Lower port radius is preferably 0.1～5mm, more preferably 1～4mm.
In this utility model, connect with funnel-shaped structure top edge and be arranged at top protrude wall be preferably hollow column structure, the highly preferred of described protrusion wall is 0.1～20mm, more preferably 0.5～5.0, most preferably 1.0mm；The wall thickness of described protrusion wall is preferably 0.1～1.0mm, more excellent for 0.3～0.8mm, most preferably 0.7mm；The radius of the column structure that described protrusion wall is hollow is preferably 0.1～5.0mm, more preferably 1.0～4.0；Protruding the effect of wall in this utility model is to prevent in experimentation the cross-contamination between ELISA Plate hole.
In this utility model, in each ELISA Plate hole, hemispherical bottom interior wall is connected with the interior walls be smooth of described funnel-shaped structure, and described funnel-shaped structure top edge inwall is connected with the described interior walls be smooth protruding wall.
The ELISA Plate provided this utility model below in conjunction with embodiment is described in detail, but they can not be interpreted as the restriction to this utility model protection domain.
Embodiment 1
In the present embodiment, the sectional view of ELISA Plate is as shown in Figure 6A, axonometric chart is as shown in Figure 7 A, side view is as shown in Fig. 5-A2, in described ELISA Plate, the setting in ELISA Plate hole is as shown in Fig. 5-A1,20 ELISA Plate holes including 4 × 5 rectangular array, the most as shown in Figure 2 A and 2B, sectional view is as it is shown on figure 3,1 be wherein ELISA Plate hole for the top view of described ELISA Plate pore structure and front view；2 is primary hemisphere；3 is secondary hemisphere；4 is funnel-shaped structure；5 is the segment shape structure of funnel-shaped structure bottom；6 is the round table-like structure on funnel-shaped structure top；7 for protruding wall.As shown in Figure 4, each ELISA Plate hole includes that hemispherical bottom and described hemispherical bottom connection are arranged at the funnel-shaped structure at middle part and connect the protrusion wall being placed in top with described funnel-shaped structure to the axonometric chart of described ELISA Plate pore structure.
The sectional view in described ELISA Plate hole as shown in Figure 6A, wherein Φ1Diameter for the primary hemisphere structure bottom ELISA Plate hole；Φ2Diameter for secondary hemisphere structure；Φ3Diameter for the segment structure of the funnel-shaped structure lower hollow in the middle part of ELISA Plate hole；H1For the secondary hemispheroidal centre of sphere beeline away from primary hemisphere basal surface；H2Height for the segment structure of the funnel-shaped structure lower hollow in the middle part of ELISA Plate hole；H3 is the height of funnel-shaped structure top frustum cone structure；H4Beeline for funnel-shaped structure lower hollow segment lower section distance ball.
Concrete hemispherical bottom, each ELISA Plate hole is provided with primary hemisphere structure and secondary hemisphere structure, and the diameter of primary hemisphere structure is set to Φ1=4.0mm, arranges 7 secondary hemisphere structures, a diameter of Φ of described secondary hemisphere structure bottom primary hemisphere2=1.0mm, the secondary hemisphere centre of sphere is away from primary hemispheroidal basal surface beeline H1Being set to 0.06mm, between neighboring secondary hemisphere, arc length is respectively set to L1=0.21mm, L2=1.16mm.
The bottom of the funnel-shaped structure in the middle part of each ELISA Plate hole is provided with a diameter of phi3=4.7738mm, highly H2=0.8mm, wall thickness δ1Segment structure hollow for=0.7mm, the beeline of the lower section distance ball of hollow segment structure is set to H4=1.01482mm, the height of funnel-shaped structure top frustum cone structure is set to H3=0.4mm, wall thickness is set to δ1=0.7mm.
The height protruding wall on top, ELISA Plate hole is set to H5=1.0mm, wall thickness is set to δ1=0.7mm.
The comparison of the surface area size in flat ELISA Plate hole (structure is as shown in Figure 1) disclosed in the surface area within ELISA Plate hole as shown in Figure 6 and prior art:
The surface area in the flat ELISA Plate hole of tradition
This example ELISA Plate hole hemispheroidal surface area of bottom primary
Bottom this example ELISA Plate hole secondary hemispheroidal surface area and
The surface area S of funnel-shaped hole bottom segment in the middle part of this example ELISA Plate holeSegment=π Φ3H2=3.81904 π (mm2)；
This example secondary hemisphere covers primary hemispheroidal surface area and SCover primary hemisphere=7 π Φ1H1=1.68 π (mm2)；
The surface area S that this example enzyme mark hole is totalAlways=SPrimary hemisphere+SSecondary hemisphere+SMiddle part segment-SCover primary hemisphere=8 π+3.5 π+3.81904 π-1.68 π ≡ 13.639 π (mm2)；
The surface area S that this example ELISA Plate hole is totalAlwaysWith the ratio of conventional holes surface area S it is
As can be seen from the above results, total surface area in ELISA Plate hole described in the utility model is 3.410 times of the surface area in traditional ELISA Plate hole, significantly increases the surface area within ELISA Plate hole.
Embodiment 2
A kind of ELISA Plate, the sectional view of described ELISA Plate is as shown in Figure 6B, axonometric chart is as shown in Figure 7 B, the side view of described ELISA Plate is as shown in Fig. 5-B2, in described ELISA Plate, the setting in ELISA Plate hole is as shown in Fig. 5-B1, and including 28 ELISA Plate holes of 4 × 7 rectangular array, the top view of described ELISA Plate pore structure and front view are as shown in Figure 2 A and 2B, sectional view is as it is shown on figure 3,1 be wherein ELISA Plate hole；2 is primary hemisphere；3 is secondary hemisphere；4 is funnel-shaped structure；5 is the segment shape structure of funnel-shaped structure bottom；6 is the round table-like structure on funnel-shaped structure top；7 for protruding wall.As shown in Figure 4, each ELISA Plate hole includes that hemispherical bottom and described hemispherical bottom connection are arranged at the funnel-shaped structure at middle part and connect the protrusion wall being placed in top with described funnel-shaped structure to the axonometric chart of described ELISA Plate pore structure.
The sectional view in described ELISA Plate hole as shown in Figure 6B, wherein Φ1Diameter for the primary hemisphere structure bottom ELISA Plate hole；Φ2Diameter for secondary hemisphere structure；Φ3Diameter for the segment structure of the funnel-shaped structure lower hollow in the middle part of ELISA Plate hole；H1For the secondary hemispheroidal centre of sphere beeline away from primary hemisphere basal surface；H2Height for the segment structure of the funnel-shaped structure lower hollow in the middle part of ELISA Plate hole；H3 is the height of funnel-shaped structure top frustum cone structure；H4Beeline for funnel-shaped structure lower hollow segment lower section distance ball.
Concrete hemispherical bottom, each ELISA Plate hole is provided with primary hemisphere structure and secondary hemisphere structure, and the diameter of primary hemisphere structure is set to Φ1=4.0mm, arranges 7 secondary hemisphere structures, a diameter of Φ of described secondary hemisphere structure bottom primary hemisphere2=1.0mm, the secondary hemispheroidal centre of sphere is away from primary hemispheroidal basal surface beeline H1Being set to 0.06mm, between neighboring secondary hemisphere, arc length is respectively set to L1=0.21mm, L2=1.16mm.
Funnel-shaped structure bottom in the middle part of each ELISA Plate hole is additionally provided with a diameter of phi3=4.7738mm, highly H2=0.8mm, wall thickness δ1Segment structure hollow for=0.7mm, the beeline of the lower section distance ball of hollow segment structure is set to H4=1.01482mm, the height of funnel-shaped structure top frustum cone structure is set to H3=0.4mm, wall thickness is set to δ1=0.7mm.The height protruding wall on top, ELISA Plate hole is set to H5=1.0mm, wall thickness is set to δ1=0.7mm.
The comparison of the surface area size in the surface area within ELISA Plate hole as shown in Figure 6B and tradition flat ELISA Plate hole:
The surface area in the flat ELISA Plate hole of tradition
This example ELISA Plate hole hemispheroidal surface area of bottom primary
Bottom this example ELISA Plate hole secondary hemispheroidal surface area and
The surface area S of funnel-shaped hole bottom segment in the middle part of this example ELISA Plate holeSegment=π Φ3H2=3.81904 π (mm2)；
This example secondary hemisphere covers primary hemispheroidal surface area and SCover primary hemisphere=7 π Φ1H1=1.68 π (mm2)；
The surface area S that this example enzyme mark hole is totalAlways=SPrimary hemisphere+SSecondary hemisphere+SMiddle part segment-SCover primary hemisphere=8 π+3.5 π+3.81904 π-1.68 π ≡ 13.639 π (mm2)；
The surface area S that this example ELISA Plate hole is totalAlwaysWith the ratio of conventional holes surface area S it is
As seen from the above embodiment, total surface area in ELISA Plate hole described in the utility model is 3.410 times of the surface area in traditional ELISA Plate hole, significantly increase the surface area within ELISA Plate hole, thus increase the chance that target substance contacts with solid phase surface target molecule, simultaneously can be with the content of less sample size detection target substance, the bottom that ligase target hole is set of ELISA Plate funnel-shaped structure described in the utility model and top, increase the surface area in ELISA Plate hole further, the setting of protrusion wall described in the utility model can prevent cross-contamination between ELISA Plate hole and ELISA Plate hole.
The above is only preferred implementation of the present utility model; it should be pointed out that, for those skilled in the art, on the premise of without departing from this utility model principle; can also make some improvements and modifications, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (10)
1. an ELISA Plate, it is characterised in that include that some ELISA Plate holes, each ELISA Plate hole include the funnel-shaped structure that hemispherical bottom connects and the protrusion wall connected with described funnel-shaped structure top edge with described hemispherical bottom top edge.
ELISA Plate the most according to claim 1, it is characterised in that described hemispherical bottom includes primary hemisphere structure and is positioned at the inwardly protruded secondary hemisphere structure of primary hemisphere inside configuration.
ELISA Plate the most according to claim 2, it is characterised in that a diameter of the 0.2～10mm of described primary hemisphere structure, wall thickness is 0.1～1.0mm.
ELISA Plate the most according to claim 2, it is characterised in that the hemispheroidal number of secondary of each primary hemisphere inside configuration is 3～50, and described secondary hemisphere structure is evenly distributed in the inner surface of primary hemisphere structure.
ELISA Plate the most according to claim 2, it is characterised in that a diameter of the 0.1～5mm of described secondary hemisphere structure, wall thickness is 0.1～1.0mm, and the interstructural hole of neighboring secondary hemisphere is 0.1～1.0mm.
ELISA Plate the most according to claim 1, it is characterized in that, described funnel-shaped structure is made up of the hollow segment and the hollow round table two parts connected with described hollow segment top edge being positioned at bottom, and the lower limb of described hollow segment shape connects with the top edge of described hemispherical bottom；The top edge of described hollow round table is connected with the lower limb of described protrusion wall.
ELISA Plate the most according to claim 6, it is characterised in that a diameter of the 0.2～10mm of described hollow segment, height is 0.1～5.0mm, and wall thickness is 0.1～1.0mm.
ELISA Plate the most according to claim 6, it is characterised in that the height of described hollow round table is 0.2～6.0mm, wall thickness is 0.1～1.0mm, and upper port radius is 0.1～5.0mm, and lower port radius is 0.1～5.0mm.
ELISA Plate the most according to claim 1, it is characterised in that described protrusion wall is hollow column structure, the height of described protrusion wall is 0.1～20mm, and wall thickness is 0.1～1.0mm, and radius is 0.1～5.0mm.
ELISA Plate the most according to claim 1, it is characterised in that the area of ELISA Plate is 10～200 × 5～200mm ELISA Plate, and each ELISA Plate preferably includes 1～500 ELISA Plate hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620233863.8U CN205426923U (en) | 2016-03-24 | 2016-03-24 | Elisa plate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620233863.8U CN205426923U (en) | 2016-03-24 | 2016-03-24 | Elisa plate |
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| CN205426923U true CN205426923U (en) | 2016-08-03 |
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| CN201620233863.8U Expired - Fee Related CN205426923U (en) | 2016-03-24 | 2016-03-24 | Elisa plate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699644A (en) * | 2016-03-24 | 2016-06-22 | 何韶衡 | ELISA (Enzyme Linked Immunosorbent Assay) plate |
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2016
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105699644A (en) * | 2016-03-24 | 2016-06-22 | 何韶衡 | ELISA (Enzyme Linked Immunosorbent Assay) plate |
| WO2017161707A1 (en) * | 2016-03-24 | 2017-09-28 | 锦州医科大学附属第一医院 | Microtiter plate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170526 Address after: 121000 No. five, Section 2, Renmin Street, Guta District, Liaoning, Jinzhou Patentee after: The First Affiliated Hospital of Jinzhou Medical University Address before: 121000 Jinzhou city of Liaoning Province Taihe District South West Ling in a beautiful day. D 35-97 Patentee before: He Shaoheng |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160803 |