CN206441437U - A kind of microchip imparted knowledge to students for TLC - Google Patents
A kind of microchip imparted knowledge to students for TLC Download PDFInfo
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- CN206441437U CN206441437U CN201621202312.1U CN201621202312U CN206441437U CN 206441437 U CN206441437 U CN 206441437U CN 201621202312 U CN201621202312 U CN 201621202312U CN 206441437 U CN206441437 U CN 206441437U
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- sample
- pipeline
- microchip
- students
- reservoir
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- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 230000000903 blocking effect Effects 0.000 claims abstract description 6
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 4
- 238000004891 communication Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 16
- 239000004205 dimethyl polysiloxane Substances 0.000 description 11
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 11
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 11
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 11
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 239000012943 hotmelt Substances 0.000 description 4
- 238000007639 printing Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000001723 curing Methods 0.000 description 3
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 229910000679 solder Inorganic materials 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 229920001875 Ebonite Polymers 0.000 description 1
- 206010022524 Intentional self-injury Diseases 0.000 description 1
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 1
- 208000005560 Self Mutilation Diseases 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- DMLAVOWQYNRWNQ-UHFFFAOYSA-N azobenzene Chemical compound C1=CC=CC=C1N=NC1=CC=CC=C1 DMLAVOWQYNRWNQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- JZZIHCLFHIXETF-UHFFFAOYSA-N dimethylsilicon Chemical compound C[Si]C JZZIHCLFHIXETF-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of microchip imparted knowledge to students for TLC, including chip body, the first sample channel and the second sample channel are provided with the chip body, first sample channel and the second sample channel go out sample pipeline communication by several of cross pipeline and its lower end, each outlet for going out sample pipeline is communicated with reservoir, and the reservoir is the dual-slot structure that middle part is provided with blocking part；The Mixed Expansion agent of different proportion then can once be formed conveniently with two kinds of pure reagents by sample introduction using this chip, the influence that different proportion solvent deploys result to sample can easily be observed by the reservoir with dual-slot structure of particular design again, it is convenient intuitively to allow student to understand solvent to RfInfluence, reach more preferable teaching efficiency.
Description
Technical field
The utility model is related to Teaching of Analytical Chemistry fixture technology field, and in particular to one kind is based on microfluidic chip technology
What is made is used for the microchip that TLC is imparted knowledge to students.
Background technology
This technology belongs to the crossing domain of miniaturization chemical reaction equipment and microfluidic chip technology, and its main thought is profit
With the manufacturing technology of micro-fluidic chip, new microization Teaching of Analytical Chemistry utensil is made, Manz A et al. were in 90 years 20th century
In generation, just proposes micro-full analytical system (miniaturized total analysis system, μ-TAS) concept first.
Micro-fluidic chip therein combine analytical chemistry, MEMS (mi-cro-electro-mechanical system,
MEMS), the multidisciplinary field such as computer, materialogy, biomedicine, by such as sample pretreatment of the various functions of chemical laboratory,
Sample introduction, separation and detection etc., are integrated on the chip of credit card-sized, realize the miniaturization in laboratory, can greatly shorten whole
Time required for individual analysis process；The consumption of reagent can be also reduced to microlitre even nanoliter level, it is possible to achieve a variety of analyses
Function.Since the beginning of this century, microfluidic chip technology is developed rapidly, and has been widely used for chip capillary electricity
Swimming, materials synthesis, immunoassay, cell operation, crystallization of protein research etc. various fields, be analysis science research focus it
One.But the technology is presently mainly to apply in terms of scientific research, teaching and research aspect applies less, but microchip method consumptive material is few, just
The features such as property taken is good exactly meets Green Chemistry, the requirement of Microscale chemical experiment, is also convenient for realizing teaching combining theory with practice, because
This its also there is application prospect widely in teaching field.On the other hand, the technology such as MEMS skills that current microchip makes
Art etc. costly, which also limits its teaching field application.
Microscale experiment is to emerge 1980s, and that generally acknowledges in the world can embody the chemical experiment of green chemical concept
New technology and method.It, which has, saves medicine, saves the time, and the advantages of reducing " three wastes " of experiment causes both at home and abroad
The most attention of experimental teaching personnel.But in general miniaturized devices need remain for the facilities such as supporting water power, it is impossible to
Really bring classroom into be tested, while instrument and equipment needs are special therefore also more expensive in price, it is impossible to realize teacher or even
The raw self-mutilation behavior to reaction unit.
Such as the teaching of TLC, the method can be tested by thin layer chromatographic cylinder, but its volume is larger,
The reagent expended required for expansion is more, and the process of configuration different proportion solvent is very cumbersome, therefore designs a be used for
The microchip of TLC teaching, it is simple to operate, do not take reagent, can intuitively allow student to understand solvent to R againfShadow
Ring, be significantly.
Utility model content
The technical problems to be solved in the utility model be design it is a kind of it is portable, directly can demonstrate thin layer on classroom
The microchip of chromatography, can reach more preferable teaching efficiency.
To reach above-mentioned purpose, the technical solution adopted in the utility model is：It is a kind of to be used for the micro- of TLC teaching
The first sample channel and the second sample channel, first sample feeding pipe are provided with chip, including chip body, the chip body
Road and the second sample channel go out sample pipeline communication by several of cross pipeline and its lower end, and each goes out the outlet of sample pipeline
Reservoir is communicated with, the reservoir is the dual-slot structure that middle part is provided with blocking part.
It is preferred that, the reservoir includes preceding groove and pit.
It is preferred that, it is described go out sample pipeline quantity be five, the reservoir going out sample pipeline exit be in a line five
Row arrangement.
It is preferred that, go out in sample pipeline to be formed the Mixed Expansion agent of different proportion described in five.
It is preferred that, the multiple pipe tunnel phases of the pipeline by three horizontal pipelines and between horizontal pipeline that cross
It is intercommunicated and constitute.
It is preferred that, the first sample channel entrance is provided with the first injection port, and the second sample channel entrance enters provided with second
Sample mouthful.
It is preferred that, it is equipped with thin-layer tablet in the pit.
The beneficial effects of the utility model are：
1st, the mixing of different proportion then can once be formed conveniently with two kinds of pure reagents by sample introduction using this chip
Solvent, then different proportion solvent can easily be observed to sample by the reservoir with dual-slot structure of particular design
Deploy the influence of result, it is convenient intuitively to allow student to understand solvent to RfInfluence, reach more preferable teaching efficiency.
2nd, cost of manufacture is cheap, without any large-scale instrument and equipment, facilitates and is mass produced, and meets teaching and uses,
Processing can be designed in common lab simultaneously, without special instrument and equipment, facilitates teacher or even student to enter relevant apparatus
Row makes.
3rd, the chip good portability prepared, can be carried to classroom and carry out teaching combining theory with practice.
4th, the chip pipeline that the printing template that compares method this method is obtained is wide, and area of display is big, and phenomenon becomes apparent from.It is simultaneously real
The chip for having showed the preparation of printing template method is difficult to the chip with three-dimensional structure prepared.
Brief description of the drawings
Accompanying drawing is, for providing a further understanding of the present invention, and to constitute a part for specification, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the schematic diagram of the utility model microchip；
Fig. 2 is the sectional view in A-A directions in Fig. 1；
Fig. 3 is the preparation process figure of microchip；
In figure：First sample channel 1；First injection port 10；Second sample channel 2；Second injection port 20；Horizontal pipeline
30；Pipe tunnel 31；Go out sample pipeline 4；Preceding groove 50；Pit 51；Thin-layer tablet 510；Blocking part 52；Chip body 6；Culture dish 7；
PDMS upper cover plates 8.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only a part of embodiment of the utility model, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belongs to the scope of the utility model protection.
Accompanying drawing 1-2 is referred to, a kind of microchip imparted knowledge to students for TLC provided for the utility model, it includes
The first sample channel 1 and the second sample channel 2 are provided with chip body 6, the chip body 6, the entrance of the first sample channel 1 is set
There is the first injection port 10, the entrance of the second sample channel 2 is provided with the second injection port 20, the sample introduction of the first sample channel 1 and second
Pipeline 2 goes out sample pipeline 4 by five of cross pipeline and its lower end and connected, and each outlet for going out sample pipeline 4 is communicated with storage
Liquid bath, arranges so that reservoir is arranged in the exit for going out sample pipeline 4 in a line five, and the reservoir is that middle part is provided with blocking part 52
Dual-slot structure, i.e. reservoir is blocked portion 52 and is separated into the preceding groove 50 and pit 51 being connected, and pit 51 is away from going out sample pipe
The direction that road 4 is exported；The pipeline that crosses is by three horizontal pipelines 30 and multiple pipe tunnels between horizontal pipeline
31 are interconnected and constitute, and three horizontal pipelines 30 are from top to bottom arranged successively, between the horizontal pipeline of upper end and centre
Provided with three pipe tunnels 31, provided with four pipe tunnels 31, the horizontal pipeline of upper end between the middle and horizontal pipeline of lower end
30 are connected with the first sample channel 1 and the second inlet channel 2, and the horizontal pipeline 30 of lower end is connected with going out sample pipeline 4.
The principle of the microchip demonstration TLC provided using the utility model is as follows：By chloroform and ethanol respectively by
First injection port 10 and the second injection port 20 are injected in the first sample channel 1 and the second sample channel 2, chloroform and ethanol mixing
Flowed into out afterwards by the pipeline that crosses in sample pipeline 4, sample is gone out because respectively reaching each by the pipeline that crosses after chloroform and ethanol mixing
The stroke that pipeline is undergone is different, so as to go out in sample pipeline 4 to be formed the chloroform of different proportion and the Mixed Expansion of ethanol at five
Agent；Meanwhile, prior addition point has the thin-layer tablet 510 of azobenzene, the chlorine of above-mentioned different proportion in the pit 51 of each group reservoir
The Mixed Expansion agent of imitative and ethanol enters the preceding groove 50 of reservoir after the outlet of sample pipeline is reached out, and saturation is picked up after 20 minutes
Microchip, which is tilted down, pours into Mixed Expansion agent in pit 51, so as to just can see different proportion from the expansion result of pit
The Rf value R that deploys to sample of Mixed Expansion agentfThe influence of value.
During the microchip of above-mentioned TLC teaching is prepared, this seminar used a variety of methods, institute
The method used is printing template method, solder stick-flour hybrid template method, and printing template this method device therefor is simple and effect
Substantially, have certain practical value, but be limited to printer limit that its area of display is relatively thin, phenomenon is not it is obvious that and only
X-Y scheme can be obtained, there is no the ability of Three-dimensional thickness.Solder stick-flour hybrid template method template is easily destroyed, and is one
Secondary property template, solder stick is not fixed easily, and these methods have certain defect.
In order to overcome the microchip in drawbacks described above, the utility model to use PUR, culture dish, magnetite for template,
Prepared with poly dimethyl silicon rubber (i.e. PDMS), reference picture 2, preparation method is as follows：
A) sample channel, inlet channel are designed in the smooth square plastic culture dish 7 in bottom, crossing pipeline and goes out sample
The position of pipeline, the pipeline model of projection is depicted with PUR at the corresponding pipeline in culture dish, i.e., with PUR edge pipe
The shape in road is coated, and waits it to solidify；
B) magnetite that sample pipeline 4 exports one piece of same thickness of placement respectively is gone out at each, for forming reservoir, training
Support and can be placed in can surface below ware, so as to by the position of magneticaction fixed magnet stone, complete that there is three dimensions
The template of structure；
C) by PDMS glue according to monomer：Curing agent=10:1 ratio is prepared, well mixed to pour into culture dish, more than magnetic
Surface where iron-stone, standing is drained after bubble, and two hours are dried at a temperature of 60 °；
D) the PDMS colloids that completion is dried in step c are stripped down from culture dish, removes magnetite, obtain PDMS bases
Confuser；
E) using hot melt glue pot heating PUR, stand the hot melt after fusing after the bubble in discharge PUR as far as possible
Glue is poured in step d in each pipeline and reservoir of PDMS confusers；
F) peeled off after after PUR solidification in step e, copy out threedimensional model, form the formpiston of hot melt matrix, and in shape
Into the end of the reservoir threedimensional model inside cut portion material of pocket knife, it is easy to form blocking part；Said process can also be adopted
PUR is replaced with crystal ebonite；
G) formpiston of hot melt matrix is put into culture dish, again by PDMS glue according to monomer：Curing agent=10:1 ratio
Prepare, it is well mixed to pour into culture dish；
H) after being peeled off after PDMS adhesive curings in step g, the pipeline of the final required PDMS bases with dual-slot structure is obtained
Piece；
I) surface of the confuser of the PDMS bases obtained in step h is adjacent to using other one piece of PDMS upper cover plate 8 sealing-in,
Chip body 6 is obtained, after being punched on upper cover plate, you can for testing.
Schematically the utility model and embodiments thereof are described above, the description does not have restricted, accompanying drawing
Shown in be also one of embodiment of the present utility model, actual structure is not limited thereto.So, if this area
Those of ordinary skill enlightened by it, do not departing from the case that the utility model creates objective, designed without creative
The frame mode similar to the technical scheme and embodiment, all should belong to protection domain of the present utility model.
Claims (7)
1. a kind of microchip imparted knowledge to students for TLC, including chip body (6), it is characterised in that：The chip body
(6) the first sample channel (1) and the second sample channel (2), first sample channel (1) and the second sample channel are provided with
(2) go out sample pipeline (4) by cross pipeline and several of its lower end to connect, each outlet for going out sample pipeline is communicated with storage
Liquid bath, the reservoir is the dual-slot structure that middle part is provided with blocking part (52).
2. a kind of microchip imparted knowledge to students for TLC according to claim 1, it is characterised in that：The reservoir
Including preceding groove (50) and pit (51).
3. a kind of microchip imparted knowledge to students for TLC according to claim 1, it is characterised in that：It is described go out sample pipe
The quantity in road (4) is five, and the reservoir is arranged in a line five in the exit for going out sample pipeline and arranged.
4. a kind of microchip imparted knowledge to students for TLC according to claim 1, it is characterised in that：Go out described in five
The Mixed Expansion agent of different proportion can be formed in sample pipeline.
5. a kind of microchip imparted knowledge to students for TLC according to claim 1, it is characterised in that：The pipe that crosses
Road is interconnected by three horizontal pipelines (30) and multiple pipe tunnels (31) between horizontal pipeline and constituted.
6. a kind of microchip imparted knowledge to students for TLC according to claim 1, it is characterised in that：Described first enters
Sample pipeline (1) entrance is provided with the first injection port (10), and the second sample channel (2) entrance is provided with the second injection port (20).
7. a kind of microchip imparted knowledge to students for TLC according to claim 2, it is characterised in that：The pit
(51) thin-layer tablet (510) is equipped with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621202312.1U CN206441437U (en) | 2016-11-08 | 2016-11-08 | A kind of microchip imparted knowledge to students for TLC |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621202312.1U CN206441437U (en) | 2016-11-08 | 2016-11-08 | A kind of microchip imparted knowledge to students for TLC |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206441437U true CN206441437U (en) | 2017-08-25 |
Family
ID=59645288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621202312.1U Expired - Fee Related CN206441437U (en) | 2016-11-08 | 2016-11-08 | A kind of microchip imparted knowledge to students for TLC |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206441437U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11484880B2 (en) | 2018-06-25 | 2022-11-01 | Lg Chem, Ltd. | Microdevice for detecting aldehydes or ketones |
-
2016
- 2016-11-08 CN CN201621202312.1U patent/CN206441437U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11484880B2 (en) | 2018-06-25 | 2022-11-01 | Lg Chem, Ltd. | Microdevice for detecting aldehydes or ketones |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170825 |