CN206293074U - A kind of langbobier law teaching microchip - Google Patents

Abstract

The utility model discloses a kind of langbobier law teaching microchip, including chip body, sample channel and inlet channel are provided with the chip body, the sample channel and inlet channel go out sample pipeline communication by the passage that crosses with four of its lower end, and the outlet that sample pipeline is gone out described in each is sequentially communicated the reservoir of multiple different-thickness；The microchip provided using the design facilitates student to understand the content of courses, is convenient for teaching combining theory with practice.

Description

A kind of Lambert-Beer's law teaching microchip

Technical field

The utility model is related to Teaching of Analytical Chemistry fixture technology field, and in particular to one kind is based on microfluidic chip technology The Lambert-Beer's law teaching microchip of making.

Background technology

This technology belongs to the crossing domain of miniaturization chemical reaction equipment and microfluidic chip technology, and its main thought is profit With the manufacturing technology of micro-fluidic chip, new microization Teaching of Analytical Chemistry utensil, Manz A et al. are made in 90 years 20th century In generation, just proposes the concept of micro-full analytical system (miniaturized total analysis system, μ-TAS) first. Micro-fluidic chip therein combine analytical chemistry, MEMS (mi-cro-electro-mechanical system, MEMS), the multidisciplinary field such as computer, materialogy, biomedicine, by such as sample pretreatment of the various functions of chemical laboratory, Sample introduction, separation and detection etc., are integrated on the chip of credit card-sized, realize the miniaturization in laboratory, can greatly shorten whole Time required for individual analysis process；Also the consumption of reagent can be reduced to microlitre even nanoliter level, it is possible to achieve various analyses Function.Since the beginning of this century, microfluidic chip technology is developed rapidly, and has been widely used for chip capillary electricity Swimming, materials synthesis, immunoassay, cell operation, crystallization of protein research etc. various fields, be analysis science research focus it One.But the technology is presently mainly to apply in terms of scientific research, and teaching and research aspect applies less, but microchip method consumptive material is few, just The features such as property taken is good exactly meets the requirement of Green Chemistry, Microscale chemical experiment, is also convenient for realizing teaching combining theory with practice, because This its also there is application prospect widely in teaching field.On the other hand, the technology such as MEMS skills that current microchip makes Art etc. costly, which also limits its teaching field application.

Microscale experiment is to emerge the eighties in 20th century, the generally acknowledged chemical experiment that can embody green chemical concept in the world New technology and method.It has saving medicine, time-consuming, reduce experiment " three wastes " the advantages of, cause both at home and abroad The most attention of experimental teaching personnel.But in general miniaturized devices need remain for the facilities such as supporting water power, it is impossible to Really bring classroom into be tested, while instrument and equipment needs are special therefore also more expensive in price, it is impossible to realize teacher or even The raw self-mutilation behavior to reaction unit.

As the teaching at present to Lambert-Beer's law needs to use ultraviolet-visible spectrophotometer, it needs supporting water Electricity, portability is poor, it is impossible to brings classroom into and is imparted knowledge to students, therefore can only be that theory and practice teaching is separated, difficult during theory teaching To give classmate's intuitively impression on the law.The expression formula of Lambert-Beer's law is：

A=KcL

Represent when a branch of collimated monochromatic ligth is perpendicular through a certain with the certain illuminating area, weak solution containing extinction material When, if the solution it is uniform, without scattering, in the case where the condition such as temperature of lambda1-wavelength, intensity and solution keeps constant, Extinction material in the solution is directly proportional pass to monochromatic absorbance A to the concentration c of solution and the product of solution liquid layer thickness L Be combined together for the technology of the concept of Microscale chemical experiment and micro-fluidic chip by system, the design, devises a kind of by heat Colloidal sol, culture dish, magnet are template, the religion that can demonstrate Lambert-Beer's law prepared with poly dimethyl silicon rubber (PDMS) Chip, use can build the microchip of concentration gradient and thickness gradient, reach more preferable teaching efficiency.

Utility model content

The technical problems to be solved in the utility model be design it is a kind of it is portable, can directly demonstrate bright on classroom The microchip of primary-Beer law.

To reach above-mentioned purpose, the technological means that the utility model is used for：Micro- core is used in a kind of Lambert-Beer's law teaching Piece, including chip body, are provided with sample channel and inlet channel in the chip body, the sample channel and inlet channel are logical Cross the passage that crosses and go out sample pipeline communication with four of its lower end, the outlet that sample pipeline is gone out described in each has been sequentially communicated multiple not The reservoir of stack pile.

Further, go out in sample pipeline to be formed the liquor potassic permanganate of various concentrations gradient described in four.

Further, the quantity that the reservoir of the outlet of sample pipeline is gone out described in any one is three.

Further, the thickness of three reservoirs is followed successively by 2mm, 4mm, 6mm.

Further, the entrance of the sample channel is provided with injection port, and the entrance of the inlet channel is provided with water inlet.

The beneficial effects of the utility model are：Cost of manufacture is cheap, without any large-scale instrument and equipment, conveniently carries out big Large-scale production, meets teaching and uses, while processing can be designed in common lab, without special instrument and equipment, convenient religion Shi Naizhi student makes to relevant apparatus.The chip good portability prepared, can be carried to classroom carries out managing real one Change teaching, facilitate classmate to understand the content of courses in time, comparing with current teaching method obviously can more allow classmate intuitively to see Observe phenomenon.The chip pipeline that the printing template that compares method the method is obtained is wide, and area of display is big, and phenomenon becomes apparent from.It is simultaneously real The chip for having showed the preparation of printing template method is difficult to the chip with three-dimensional structure for preparing, and facilitates the teaching to relevant knowledge.

Brief description of the drawings

Accompanying drawing is, for providing a further understanding of the present invention, and to constitute the part of specification, with following tool Body implementation method is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings：

Fig. 1 is schematic diagram of the present utility model；

Fig. 2 is the preparation process schematic diagram of microchip；

In figure：Sample channel 1；Inlet channel 2；Cross pipeline 3；Go out sample pipeline 4；Reservoir 5；Chip body 6；Culture dish 7；PDMS lower master films 8；Injection port 10；Water inlet 20.

Structure of the present utility model, preparation method and teaching process are done into one below in conjunction with the drawings and specific embodiments Step detailed description.

Specific embodiment

As shown in figure 1, the schematic diagram of the microchip provided for the design, it includes being set in chip body 6, chip body 6 The entrance for having sample channel 1 and inlet channel 2, sample channel 1 is provided with injection port 10, and the entrance of inlet channel 2 is provided with water inlet 20, certain density potassium permanganate and pure water can be injected to sample channel 1 and water inlet pipe from injection port 10 and water inlet 20 respectively In road 2, gone out in sample pipeline 4 into four by the pipeline 3 that crosses after potassium permanganate and pure water mixing, and go out sample pipe described in any one The outlet in road is sequentially communicated the reservoir 5 of multiple different-thickness, and the pipeline 3 that crosses is by two horizontal pipelines and positioned at horizontal pipeline Between pipe tunnel be interconnected and constitute, upper end horizontal pipeline is connected with sample channel 1 and inlet channel 2, lower end horizontal stroke It is connected with sample pipeline 4 is gone out to pipeline；In the design, the quantity for going out the reservoir 5 of the outlet connection of sample pipeline 4 at each is equal It it is three, so as to go out the reservoir of the outlet three rows and four columns formed below of sample pipeline at four, and each goes out sample pipeline lower section The thickness of reservoir is incremented by successively, can be respectively set to 2mm, 4mm, 6mm, again by crossing after mixing with pure water because of potassium permanganate The stroke that pipeline arrives separately at out the outlet experience of sample pipeline is different, so that going out various concentrations gradient can be formed in sample pipeline Liquor potassic permanganate.

During the microchip of above-mentioned Lambert-Beer's law teaching is prepared, this seminar used various methods, The method for being used is printing template method, and the method device therefor is simple and effect is obvious, has certain practical value, but limit In printer to limit its area of display relatively thin, phenomenon be not it is obvious that and X-Y scheme can only be obtained, there is no three-dimensional thickness The ability of degree, it is impossible to for the teaching of Lambert-Beer's law.Solder stick-flour hybrid template method template is easily destroyed, and is once Property template, solder stick is not fixed easily, and these methods have certain defect.

For drawbacks described above, it is template that the microchip in the design uses PUR, culture dish, magnetite, uses poly- diformazan Prepared by base silicon rubber (i.e. PDMS), reference picture 2, and preparation method is as follows：

1) sample channel, inlet channel are designed in the smooth square plastic culture dish 7 in bottom, crossing pipeline and goes out sample The position of pipeline, depicts the pipeline model of projection at the corresponding pipeline with PUR in culture dish, i.e., with PUR along pipe The shape in road is coated, and waits it to solidify；

2) go out sample pipe outlet at each and be sequentially placed three groups of magnetites of varying number, its arrangement is from going out sample pipeline It is respectively 1 piece, the 2 pieces and 3 pieces accumulation of magnetite to be exported to away from the direction for exporting, and is connected with PUR between this three groups of magnetites It is logical；Can surface is can be placed in below culture dish, so as to by the position of magneticaction fixed magnet stone, complete that there is three-dimensional The template of space structure；

3) by PDMS glue according to monomer：Curing agent=1:10 part by weight is prepared, well mixed to pour into culture dish, is surpassed Surface where crossing highest magnetite, after standing drains bubble, dries two hours at a temperature of 60 °；

4) the PDMS colloids for completing will be dried to be stripped down from culture dish, magnetite is removed；

5) lower surface of PDMS colloids in step 4 is adjacent to using the sealing-in of other one piece of PDMS lower master film 8, chip sheet is obtained Body, you can for testing, the chip body for preparing can copy pattern and prepare new template by thermosol, it is ensured that the weight of chip Existing property.

During experiment, certain density potassium permanganate and pure water are injected to sample feeding pipe from injection port 10 and water inlet 20 respectively In road 1 and inlet channel 2, the concentration gradient of potassium permanganate is formed so as to the outlet for going out sample pipeline 4 at four, meanwhile, any It is individual go out sample pipeline 4 exit, the reservoir 5 being arranged in order will be formed different-thickness absorption cell, it is high using monochromatic light exposure Potassium manganate solution, because liquor potassic permanganate is to monochromatic absorbing state, because various concentrations gradient and different absorption cells are thick The difference of degree, can be presented the change of the depth in color, so as to help classmate to understand the extinction of different solutions by this microchip Degree is proportional with solution concentration and absorption cell thickness.

Schematically the utility model and embodiments thereof are described above, the description does not have restricted, accompanying drawing Shown in be also one of implementation method of the present utility model, actual structure is not limited thereto.So, if this area Those of ordinary skill enlightened by it, do not departing from the case that the utility model creates objective, designed without creative The frame mode similar to the technical scheme and embodiment, all should belong to protection domain of the present utility model.

Claims (5)

1. a kind of Lambert-Beer's law teaching microchip, including chip body (6), it is characterised in that：The chip body (6) sample channel (1) and inlet channel (2) are provided with, the sample channel (1) and inlet channel (2) are by the passage that crosses (3) Go out sample pipeline (4) with four of its lower end to connect, the outlet that sample pipeline is gone out described in each has been sequentially communicated multiple different-thickness Reservoir (5).

2. a kind of Lambert-Beer's law teaching microchip according to claim 1, it is characterised in that：Go out described in four The liquor potassic permanganate of various concentrations gradient can be formed in sample pipeline.

3. a kind of Lambert-Beer's law teaching microchip according to claim 1, it is characterised in that：Described in any one The quantity for going out the reservoir of the outlet of sample pipeline is three.

4. a kind of Lambert-Beer's law teaching microchip according to claim 3, it is characterised in that：Three storages The thickness of liquid bath is followed successively by 2mm, 4mm, 6mm.

5. a kind of Lambert-Beer's law teaching microchip according to claim 1, it is characterised in that：The sample feeding pipe The entrance in road is provided with injection port (10), and the entrance of the inlet channel is provided with water inlet (20).