CN107661784A - After a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip - Google Patents
After a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip Download PDFInfo
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- CN107661784A CN107661784A CN201711105886.6A CN201711105886A CN107661784A CN 107661784 A CN107661784 A CN 107661784A CN 201711105886 A CN201711105886 A CN 201711105886A CN 107661784 A CN107661784 A CN 107661784A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
- B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
- B01F33/3017—Mixing chamber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The present invention provide after a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip, including upper strata cover plate, intermediate layer elastic film, underlying basal, upper strata cover plate include pneumatic micro valve control passage；Underlying basal includes inlet, liquid outlet, fluid passage air inlet, liquid storage tank, waste liquid pool, fluid passage, impedance transducer, absorbance TCH test channel.The openend connecting line of control passage, pipeline connection magnetic valve, magnetic valve connection vacuum system and pressure system, the PDMS film below controllable channel raise up or oppressed downwards fluid passage, and are controlled and switched with software.Fluid passage air inlet connects gas bomb or syringe；Inlet connecting line, pipeline connection syringe pump.Prestore lytic agent and dilution respectively in two liquid storage tanks.Quantitative accurate of the present invention, a kind of mixing of large scale sample is realized, it is as a result accurate reliable, provide a kind of brand-new technology for the quantitative shunting of miniflow liquid solution.
Description
Technical field
The present invention relates to a kind of chip structure, more particularly to being pre-stored in interior liquid hybrid detection after a kind of quantitative shunting
Micro-fluidic chip.
Background technology
Micro-fluidic chip has small consumption, big flux, high efficiency and low cost etc. excellent as a kind of new test device
Point, have begun to be applied to the fields such as chemical analysis, biological detection and environmental monitoring.It is how real in microfluidic analysis chip
The now quantitative shunting to microfluid is always the research puzzle and focus in microfluidic analysis chip technology in recent years.Microfluid
Quantitative sample injection is sample treatment and the key of analysis, such as with regard to as needs in biologicall test, phase transformation reaction, multi-component reaction
Operation.Some of which method can realize quantitative shunting, such as electricity analogy method, the extraining sampling method based on cross aisle.
But electricity analogy method can only be used as an approximate solution method, it is impossible to accurately design high-precision fluid network；With ten
Extraining sampling method passage domestic demand based on word passage adds buffer solution, influences the concentration of sample, while the constituent of solution is not
Together, the different ion constituent of mobility is produced sample introduction and discriminate against effect, be subject to certain restrictions this method.Therefore fixed
It is precisely, reliably, without influence sample urgent need indispensability to measure shunting aspect.
Micro-mixer is as the main function components on micro-fluidic chip, and for realizing, two or more flow under miniature scale
Quick mixing, the reaction of body, and realize desired testing result.Solute is mixed with two mechanism：One is convective mass transfer, and one is
Diffusion mass transfer.However, at present mixing plurality of liquid be typically be injected into simultaneously by multiple passages Lai, or in micro-fluidic chip
Inside prestore freeze-dried powder.And on prestored in micro-fluidic chip liquid and with shunting and the document that is mixed of liquid that comes is then relative
It is very few.
The content of the invention
The invention aims to provide after a kind of quantitative shunting with being pre-stored in the micro-fluidic of interior liquid hybrid detection
Chip, be a kind of method for quantitatively shunting after sample is injected in micro-fluidic chip, and realize the sample after making shunting with it is pre-
A series of detection after the liquid in micro-fluidic chip mixes be present.
The object of the present invention is achieved like this：Including upper strata cover plate, intermediate layer elastic film, underlying basal, it is described on
Four through holes are respectively arranged with the same position of layer cover plate and intermediate layer elastic film, underlying basal is provided with four grooves,
And the position of groove and the position correspondence of through hole, four through hole upper ends of the upper strata cover plate are respectively arranged with circular wall, upper strata
Four through holes of cover plate and intermediate layer elastic film, four grooves, four circular walls of underlying basal form the first liquid storage tank,
Second liquid storage tank, the first waste liquid pool, the second waste liquid pool, the lower surface of the upper strata cover plate are provided with first to fourth pneumatic micro valve
Control passage, the openend of four pneumatic micro valve control passages are connected with exterior line, the upper surface difference of the underlying basal
Be provided with connection the first liquid storage tank and the first waste liquid pool fluid passage, connection the second liquid storage tank and the second waste liquid pool pipeline and
Absorbance TCH test channel, first to second liquid channel inlet mouth, inlet, liquid outlet, the first fluid passage air inlet, second
Fluid passage air inlet is connected by first passage and second channel with the first liquid storage tank, the second liquid storage tank respectively, inlet with
Second liquid passage connects, and liquid outlet connects with first passage and second channel simultaneously, the upper strata cover plate, intermediate layer Thin Elastic
It is respectively arranged with film and the first through hole communicated to second liquid channel inlet mouth, inlet, liquid outlet, the first liquid storage of connection
An impedance is respectively arranged with the fluid passage and the pipeline of the second liquid storage tank of connection and the second waste liquid pool of pond and the first waste liquid pool
Sensor.
Present invention additionally comprises some such architectural features：
1. being provided with dilution in the first liquid storage tank, lytic agent is provided with the second liquid storage tank.
2. the aperture that the impedance transducer includes being arranged on underlying basal, the electrode for being arranged on aperture both sides.
3. the absorbance TCH test channel is provided in the groove on underlying basal, the two sides of the groove are and bottom surface
The inclined-plane of angle at 45 °.
4. the material of the upper strata cover plate and underlying basal is glass, the material of middle intermediate layer elastic film is poly- diformazan
Radical siloxane.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention proposes one kind in micro-fluidic chip quantification
The device of shunting, quantitative accurate is noiseless to the sample chemical composition in chip, facilitate detection of the sample in chip, reaction,
The operation such as mixing.A kind of mixing of large scale sample two-by-two is realized simultaneously, realizes the testing impedance of solution and suction after mixing
Luminosity measurement.The chip of the present invention can realize quantitative sample injection to solution, the sample size of solution can be controlled in microlitre rank or
Nanoliter rank.Shunting can be different according to the amount of shunting and shape, size are different.
Brief description of the drawings
Fig. 1 is that (dotted line represents that the passage of lower surface shows to micro-fluidic chip upper strata cover plate structural representation of the invention in figure
It is intended to)；
Fig. 2 is the intermediate layer elastic film structures schematic diagram of the present invention；
Fig. 3 is the underlying basal structural representation of the present invention；
Fig. 4 is the overall structure overlook direction schematic diagram of the present invention；
Fig. 5 is the three-dimensional structure view of the present invention；
Fig. 6 is partial enlarged drawing at I in Fig. 4；
Fig. 7 is partial enlarged drawing at II in Fig. 3 (a, b represent the position of two electrodes respectively)；
Fig. 8 is absorbance TCH test channel profile.
In figure：1 is upper strata cover plate；2nd, 3,4,5 be first to fourth pneumatic micro valve control passage；6 be intermediate layer Thin Elastic
Film；7 be underlying basal；8 be inlet；9 be liquid outlet；11st, 10 be first to second liquid channel inlet mouth；12nd, 13 be the
One and second liquid storage tank；14th, 15 be the first and second waste liquid pools；16 be fluid passage；17 be impedance transducer；18 be absorbance
TCH test channel；A, it is insertion electrode position at b.
Embodiment
The present invention is described in further detail with embodiment below in conjunction with the accompanying drawings.
As shown in Fig. 1,2,3,4,5, the invention provides after a kind of quantitative shunting with being pre-stored in interior liquid hybrid detection
Micro-fluidic chip, totally three layers, including upper strata cover plate 1, intermediate layer elastic film 6, underlying basal 7.Described chip uses three
Layer chip is formed by stacking, and by the alignment of each layer micro-fluidic chip, bonding, pressurizing and sealing, forms the micro-structural with 3 D stereo.
Upper strata cover plate 1 includes four pneumatic micro valve control passages 2,3,4,5；Underlying basal 7 includes inlet 8, liquid outlet
9th, fluid passage air inlet 10,11, the first to the second liquid storage tank 12,13, the first to the second waste liquid pool 14,15, fluid passage 16,
Impedance transducer 17, absorbance TCH test channel 18.
The material that described cover plate 1 and substrate 7 select can be glass, the material selection poly- two of intermediate layer elastic film 6
Methylsiloxane PDMS.
Inlet 8, liquid outlet on described substrate 7, the size of fluid passage air inlet control passage openend use
A diameter of 0.8mm circle, different form and dimensions can be also arranged to according to different device or processing conditions and is only limitted to this
Embodiment, other embodiment voluntarily changes according to demand, still within the scope of the present invention.
Described impedance transducer 17 needs individually to process and be bonded on the underlying basal 7 of chip.Described impedance passes
The aperture that sensor is diameter 70um, length is 100um, as shown in fig. 7, the both sides of aperture are put into electrode, the position of electrode is placed on
Width is 2mm, length is on 1mm passage, and electrode is preferably printed on chip, if after insert, sealing will get well, entirely
Impedance transducer needs individually to process and be bonded on the underlying basal of chip.
The optical channel that described absorbance TCH test channel 18 is a diameter of 2mm, length is 10mm, as shown in figure 8, passage two
End is designed with two triangular grooves, and groove surfaces and channel plane are in 45° angle.
Described liquid storage tank 12,13, waste liquid pool 14,15 need to be bonded on cover plate 1, and height is at least 5mm, a diameter of
15mm, and upper end open is kept, in air.Wherein, what liquid storage tank 12 filled is 3ml dilution, what liquid storage tank 13 filled
It is 2ml lytic agent.
The openend connecting line of described control passage 2, pipeline connection magnetic valve, magnetic valve connection vacuum system and pressure
Force system, controlled in switching figure and be not drawn into software, fluid passage air inlet 10,11 connects gas bomb or syringe；Enter
The connecting line of liquid mouth 8, pipeline connection syringe pump；When control passage leads to pressure, the intermediate layer PDMS film below passage is oppressed,
And then the fluid passage of underlying basal is oppressed, hinder the flowing of liquid.When control passage leads to vacuum, intermediate layer below passage
PDMS film can raise up, and enter but the fluid passage of underlying basal keeps clear.Fluid passage air inlet connects gas steel
Bottle or syringe；Inlet connecting line, pipeline connection syringe pump.
Described micro-fluidic chip, this chip can realize the quantitative sample injection to solution, and the sample size of solution is can be controlled in
Microlitre rank or nanoliter rank.Shunting can be different according to the amount of shunting and shape, size are different.Present case is using chip
Two parts volume 0.1um and 10um are split into after drawing sample, as shown in fig. 6, fluid passage depth is 0.5mm, width is
0.4mm, AB section black shaded area total length are 50mm, and CD section black shaded areas length is 0.5mm.Wherein, 0.1um and
3ml dilution is well mixed, and 10um and 2ml lytic agent are well mixed.
Hole on described upper strata cover plate 1 and intermediate layer elastic film 6 is through hole, control passage 2 in cover plate 1,3,
4th, the fluid passage 17 in substrate 7 corresponding to 5 uses arc, and remaining fluid passage 16 uses rectangle.Between rectangle and arc
It is in smoothing junction.
Concrete operations are：First, pneumatic micro valve control passage 2,3,4,5 leads to pressure, makes PDMS film compressing below passage
Corresponding fluid passage, is separately added into 3ml dilution and 2ml lytic agent in liquid storage tank 12 and 13, keeps storage during addition
Liquid pool 12, the ventilation of 13 openings.So dilution and lytic agent is just pre-stored in chip.Then, using syringe pump in inlet 8
Place is initially added into sample, in the presence of pneumatic micro valve, flows through AB, CD and arrives liquid outlet 9 again, as shown in fig. 6, wherein AB sections black
The sample of dash area is exactly 10um, and cross junction CD section black shaded areas are exactly 0.1um.Now, using adhesive tape
Inlet 8 and liquid outlet 9 are subjected to fast sealing.Operated pneumatic valve control passage 3 and 4 leads to vacuum, makes the PDMS below passage thin
Mould is raised, makes the fluid passage of corresponding lower section open-minded.Fluid passage air inlet 10,11 is passed through gas, by the sample and CD of AB sections
Segment mark is originally pushed into liquid storage tank 13 and 12 respectively.Continue body of ventilating after push-in, the sample after two shuntings is being prestored 3ml's respectively
The rolling of dilution liquid storage tank 12 and the 2ml that the prestores turning in of lytic agent liquid storage tank 13, carries out fully contact mixing.After a period of time, then
By the openend adhesive tape fast sealing of liquid storage tank 12 and 13.Pneumatic micro valve control passage 2 and 5 leads to vacuum, makes below passage
The thin moulds of PDMS it is raised, make corresponding to fluid passage it is open-minded.The liquid mixed is separately flowed into respective impedance transducer, electricity
Pole can be connected by wire with main frame, and the sample after dilution or dissolving flows through from aperture, can be surveyed by two interelectrode impedances
The volume of the particle flowed through from aperture is measured, so as to be counted.Pass through the liquid of two impedance transducer apertures in measurement process
Volume is no less than 200uL.Wherein, the sample after dilution is flowed into waste liquid pool 14 after impedance measurement is completed, the mark after dissolving
The optical channel that this is flowed into a diameter of 2mm again after impedance measurement, length is 10mm carries out absorbance test, by built in main frame
Light source and sensor come measure dissolving after sample absorbance, finally flow into waste liquid pool 15.
To sum up, patent of the present invention is related to after a kind of quantitative shunting and is pre-stored in the micro-fluidic core of interior liquid hybrid detection
Piece, totally three layers, including upper strata cover plate, intermediate layer elastic film, underlying basal.Upper strata cover plate includes pneumatic micro valve control passage；
Underlying basal includes inlet, liquid outlet, fluid passage air inlet, liquid storage tank, waste liquid pool, fluid passage, impedance transducer, suction
Luminosity measurement passage.The openend connecting line of control passage, pipeline connection magnetic valve, magnetic valve connection vacuum system and pressure
System, the PDMS film below controllable channel raise up or oppressed downwards fluid passage, and are controlled and switched with software.Liquid
Body channel inlet mouth connects gas bomb or syringe；Inlet connecting line, pipeline connection syringe pump.In two liquid storage tanks
Prestore lytic agent and dilution respectively.Using pneumatic micro valve by add come sample be quantitatively split into two parts, and respectively with advance
The lytic agent and dilution deposited are mixed, impedance measurement and absorbance measuring.Quantitative accurate of the present invention, realize a kind of big ratio
The mixing of example sample, it is as a result accurate reliable, provide a kind of brand-new technology for the quantitative shunting of miniflow liquid solution.
Claims (9)
1. after a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip, it is characterised in that：Including upper strata
Cover plate, intermediate layer elastic film, underlying basal, set respectively in the same position of the upper strata cover plate and intermediate layer elastic film
There are four through holes, underlying basal is provided with four grooves, and the position correspondence of the position of groove and through hole, the upper strata cover plate
Four through hole upper ends are respectively arranged with circular wall, four through holes of upper strata cover plate and intermediate layer elastic film, the four of underlying basal
Individual groove, four circular walls form the first liquid storage tank, the second liquid storage tank, the first waste liquid pool, the second waste liquid pool, the upper strata lid
The lower surface of piece is provided with first to fourth pneumatic micro valve control passage, openend and the outside of four pneumatic micro valve control passages
Pipeline connects, the upper surface of the underlying basal be respectively arranged with the first liquid storage tank of connection and the first waste liquid pool fluid passage,
Connect the second liquid storage tank and the second waste liquid pool pipeline and absorbance TCH test channel, first to second liquid channel inlet mouth, enter
Liquid mouth, liquid outlet, the first fluid passage air inlet, second liquid channel inlet mouth respectively by first passage and second channel with
First liquid storage tank, the connection of the second liquid storage tank, inlet connects with second liquid passage, liquid outlet and meanwhile with first passage and second
Passage connects, be respectively arranged with the upper strata cover plate, intermediate layer elastic film with first to second liquid channel inlet mouth, enter
The through hole that liquid mouth, liquid outlet communicate, connect the first liquid storage tank and the first waste liquid pool fluid passage and the second liquid storage tank of connection and
An impedance transducer is respectively arranged with the pipeline of second waste liquid pool.
2. after a kind of quantitative shunting according to claim 1 be pre-stored in liquid hybrid detection micro-fluidic chip,
It is characterized in that：Dilution is provided with first liquid storage tank, lytic agent is provided with the second liquid storage tank.
3. after a kind of quantitative shunting according to claim 1 or 2 be pre-stored in liquid hybrid detection micro-fluidic core
Piece, it is characterised in that：Aperture that the impedance transducer includes being arranged on underlying basal, it is arranged on the electrodes of aperture both sides.
4. after a kind of quantitative shunting according to claim 1 or 2 be pre-stored in liquid hybrid detection micro-fluidic core
Piece, it is characterised in that：The absorbance TCH test channel is provided in the groove on underlying basal, the two sides of the groove be with
The inclined-plane of bottom surface angle at 45 °.
5. after a kind of quantitative shunting according to claim 3 be pre-stored in liquid hybrid detection micro-fluidic chip,
It is characterized in that：The absorbance TCH test channel is provided in the groove on underlying basal, and the two sides of the groove are and bottom
The inclined-plane of face angle at 45 °.
6. after a kind of quantitative shunting according to claim 1 or 2 be pre-stored in liquid hybrid detection micro-fluidic core
Piece, it is characterised in that：The material of the upper strata cover plate and underlying basal is glass, and the material of middle intermediate layer elastic film is poly-
Dimethyl siloxane.
7. after a kind of quantitative shunting according to claim 3 be pre-stored in liquid hybrid detection micro-fluidic chip,
It is characterized in that：The material of the upper strata cover plate and underlying basal is glass, and the material of middle intermediate layer elastic film is poly- two
Methylsiloxane.
8. after a kind of quantitative shunting according to claim 4 be pre-stored in liquid hybrid detection micro-fluidic chip,
It is characterized in that：The material of the upper strata cover plate and underlying basal is glass, and the material of middle intermediate layer elastic film is poly- two
Methylsiloxane.
9. after a kind of quantitative shunting according to claim 5 be pre-stored in liquid hybrid detection micro-fluidic chip,
It is characterized in that：The material of the upper strata cover plate and underlying basal is glass, and the material of middle intermediate layer elastic film is poly- two
Methylsiloxane.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711105886.6A CN107661784A (en) | 2017-11-10 | 2017-11-10 | After a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711105886.6A CN107661784A (en) | 2017-11-10 | 2017-11-10 | After a kind of quantitative shunting be pre-stored in liquid hybrid detection micro-fluidic chip |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030368A (en) * | 2018-09-08 | 2018-12-18 | 重庆科技学院 | Micro-fluidic chip application method associated with a kind of and cuvette |
| CN110597328A (en) * | 2019-09-18 | 2019-12-20 | 重庆大学 | Flow cooperative control system based on liquid crystal temperature control micro valve |
| WO2023213266A1 (en) * | 2022-05-05 | 2023-11-09 | 苏州锐讯生物科技有限公司 | Micro-fluidic chip and high-throughput nano-particle synthesis system based on micro-fluidic technology |
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| CN101382538A (en) * | 2007-09-04 | 2009-03-11 | 财团法人工业技术研究院 | Auto-shunting microfluid apparatus |
| CN102671729A (en) * | 2012-05-07 | 2012-09-19 | 博奥生物有限公司 | Micro-fluidic chip for multi-index biochemical detection |
| CN205127987U (en) * | 2015-09-24 | 2016-04-06 | 基蛋生物科技股份有限公司 | Micro -fluidic chip for multi -index detection |
| CN205127986U (en) * | 2015-09-24 | 2016-04-06 | 基蛋生物科技股份有限公司 | Many indexs of ration reposition of redundant personnel detect micro -fluidic chip |
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2017
- 2017-11-10 CN CN201711105886.6A patent/CN107661784A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101382538A (en) * | 2007-09-04 | 2009-03-11 | 财团法人工业技术研究院 | Auto-shunting microfluid apparatus |
| CN102671729A (en) * | 2012-05-07 | 2012-09-19 | 博奥生物有限公司 | Micro-fluidic chip for multi-index biochemical detection |
| CN205127987U (en) * | 2015-09-24 | 2016-04-06 | 基蛋生物科技股份有限公司 | Micro -fluidic chip for multi -index detection |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030368A (en) * | 2018-09-08 | 2018-12-18 | 重庆科技学院 | Micro-fluidic chip application method associated with a kind of and cuvette |
| CN109030368B (en) * | 2018-09-08 | 2023-06-23 | 重庆科技学院 | Use method of microfluidic chip combined with cuvette |
| CN110597328A (en) * | 2019-09-18 | 2019-12-20 | 重庆大学 | Flow cooperative control system based on liquid crystal temperature control micro valve |
| WO2023213266A1 (en) * | 2022-05-05 | 2023-11-09 | 苏州锐讯生物科技有限公司 | Micro-fluidic chip and high-throughput nano-particle synthesis system based on micro-fluidic technology |
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