CN102698672B - Microfluidic reactor - Google Patents

Microfluidic reactor Download PDF

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CN102698672B
CN102698672B CN 201210159002 CN201210159002A CN102698672B CN 102698672 B CN102698672 B CN 102698672B CN 201210159002 CN201210159002 CN 201210159002 CN 201210159002 A CN201210159002 A CN 201210159002A CN 102698672 B CN102698672 B CN 102698672B
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reaction
base
point
provided
reaction cell
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CN102698672A (en )
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刘正春
王宏银
吴灶全
邬燕琪
杨飞鹏
徐友志
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中南大学
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Abstract

本发明公开了一种微流体反应器,包括基座、反应池板、玻片、橡胶圈和旋盖。 The present invention discloses a microfluidic reactor comprising a base, the reaction cell plate, slide, capping and rubber ring. 所述基座、反应池板和玻片一起构成了完整的反应室,并通过所述橡胶圈缓冲由所述旋盖水平压在玻片上将反应室密闭。 Said base plate and a reaction cell together form a slide complete the reaction chamber, and through the ring buffer by the rubber cap on the level of pressure in the reaction chamber sealed slide. 本发明通过所述反应池板上梭形反应池的仿生学设计,实现了反应室中流体流动时流场分布均匀,排出时无残液,且不受反应器的放置方位影响,同时还可以用于需要温控和光照的反应。 The present invention, by designing the reaction vessel bionics spindle reaction cell plate, to achieve uniform flow distribution of the fluid flows in the reaction chamber, no residue is discharged, and not affect the orientation of the reactor is placed, but also can the reaction temperature & need for illumination. 本发明可广泛用于生物、化学、医药等各领域的微流体反应实验。 The present invention can be widely used in various fields microfluidic biological, chemical, pharmaceutical and other response experiment.

Description

一种微流体反应器 A micro fluidic reactor

技术领域 FIELD

[0001] 本发明公开了一种微流体反应器,特别涉及一种流场分布均匀、无残液、可温控的微流体反应器。 [0001] The present invention discloses a microfluidic reactor, and more particularly relates to a streaming uniform field distribution, no residue, temperature controlled microfluidic reactors. 属于微流体生化反应仪器制备技术领域。 Belonging to the technical field of microfluidic device prepared in biochemical reactions.

背景技术 Background technique

[0002] 微流体反应器作为微流体生物化学反应的主要场所,其性能好坏直接影响到反应的结果。 [0002] The microfluidic reactors as the main place microfluidic biochemical reactions, its performance directly affects the result of the reaction. 完整的生化反应中,一般都需要在同一反应室中进行多种试剂交替充换的多步骤反应,但微量的液体进入反应室时由于截面积的变化引起流速的改变,而且在反应室中流动时,会受到磨擦阻力,加上一些毛细虹吸作用,很容易造成流体流速不均,流场分布不一致,使流体充入反应室后造成浓度不均甚至无法充满,而且从反应室中排出时有残液,造成试剂的交叉污染,影响反应结果。 Full biochemical reactions, generally need to be more reagents alternately charge for multi-step reaction in the same reaction chamber, but when the amount of liquid entering the reaction chamber due to changes in cross-sectional area causes a change in flow rate, and flows in the reaction chamber , the frictional resistance will be, plus some capillary suction action, the fluid flow rate is likely to cause uneven flow distribution inconsistent, after filling the fluid into the reaction chamber can not be filled even cause density unevenness, and there is discharged from the reaction chamber residue, cross-contamination of reagents, reaction results. 为缓解残液问题,现有反应器中一般将反应池设计成垂直或倾斜一定角度的方位放置,如中国发明专利(申请号200510024234.0)公开了一种加快生物芯片微流体反应的装置,其中所用反应舱的入口与出口连线必须有一定的角度,否则空气不能推动液体流动并产生气泡。 To alleviate residue problems, the conventional reactor, typically the reaction vessel designed as a vertical or inclined at an angle of orientation is placed, as Chinese Patent (Application No. 200510024234.0) discloses a device for accelerating microfluidic reactor biochip, wherein the used the reaction chamber inlet and outlet connections must have a certain angle, or air and liquid flow can not promote bubble generation. 但是在一些需要把反应池水平放置的实验中,这种类型的反应池不能满足要求。 However, in some experiments need to be placed in the horizontal reaction vessel, the reaction vessel of this type can not meet the requirements. 基于此,有必要设计一种不受放置方位影响,实现反应室中流体流动时流场分布均匀,排出时无残液的微流体反应器。 Based on this, it is necessary to design a place not affect the orientation, to achieve uniform flow distribution of fluid flow in the reaction chamber, is discharged microfluidic residue free reactor.

发明内容 SUMMARY

[0003] 本发明的目的在于克服现有技术之不足而提供一种结构简单合理、加工制造容易、不受放置方位影响,实现反应室中流体流动时流场分布均匀,排出时无残液的微流体反应器。 [0003] The object of the present invention is to overcome the disadvantages of the prior art and to provide a simple structure and reasonable, easy manufacturing, placement position is not affected to achieve uniform flow distribution of the fluid flows in the reaction chamber, is discharged residue free The microfluidic reactor.

[0004] 本发明一种微流体反应器,包括:基座、反应池板、玻片、橡胶圈和旋盖,所述基座上依次叠置有反应池板、玻片,所述旋盖旋装在所述基座上并通过所述橡胶圈压装在所述玻片上,其特征在于:所述反应池板中部设有一类似梭形的反应池,所述反应池长度方向的两端各设有一个进液孔、出液孔,所述进液孔、出液孔与设于基座上的进液通道或出液通道相对应。 [0004] A microfluidic reactor according to the present invention, comprising: a base, the reaction cell plate, slides, rings and rubber capping, the reactions are stacked successively on the base pool plate, slide, said cap screwed on said base and through said rubber ring mounted on said press slide, characterized in that: said reaction vessel is provided with a plate similar to the central spindle of the reaction cell, said reaction cell ends in the longitudinal direction each hole is provided with a liquid inlet, a liquid inlet, a liquid inlet hole, and the fluid passage outlet hole provided in the inlet passage or a corresponding base.

[0005] 本发明一种微流体反应器中,所述反应池形状中心对称,中间宽,两端窄,类似梭形;所述反应池的两端与进液孔或出液孔轮廓相交于点C、C'、D、D',形成反应池的进液口或出液口,所述进液口或出液口的宽度CC'或DD'为:0.2-6_;反应池中部的轮廓线为直线AB及直线A' B',直线AB或直线A' B'的长度为2_60mm,直线AB与直线A' B'之间的间距为2-60mm;所述直线AB或直线A' B'两端分别通过圆弧BC、AD或B' C'、A1 D'、与反应池的端点C、D或C'、D'连接;所述圆弧BC、AD、B' C'、k' D'中,圆弧BC或B' Ci为过点C、B、E三点或点C1、B'、Ei三点并与直线AB或Ai B'相切的圆弧,所述点E或Ei是点C或Ci以B B'为轴的对称点;圆弧AD或A' Di为过点D、A、F三点或D,、A,、F,三点并与直线AB或A, B,相切的圆弧,所述点F或F,是点D或D,以AA'为轴的对称点。 [0005] A microfluidic reactor according to the present invention, the reaction vessel centrosymmetric shape, intermediate width, narrow ends, similar to the spindle; the reaction ends with liquid inlet hole or outlet hole pool contour intersects points C, a width C ', D, D', forming a liquid inlet or liquid outlet of the reaction vessel, the liquid inlet or liquid outlet CC 'of or DD' is: 0.2-6_; middle reaction vessel contour between the straight line AB and a straight line a 'B', 'B' or the length of the straight line a of AB 2_60mm, lines AB and a 'B' pitch of 2-60mm; the straight line AB or a 'B 'ends respectively, through the arc BC, AD or B' C ', A1 D', the end of the reaction cell C, D or C ', D'; said arc BC, AD, B 'C', k 'D', the arc BC or B 'Ci too point C, B, E, or three o'clock point C1, B', Ei three o'clock and the straight line AB or Ai B 'arc tangent to the point E Ei is a point or a Ci to C or B B 'axis point of symmetry; arc AD or a' Di too point D, a, F three- or D ,, a ,, F, or three o'clock and the straight line AB a, B, arc tangent, the point F, or F, D or D is a point, to AA 'axis point of symmetry. [0006] 本发明一种微流体反应器中,所述点A、B、C、D、A'、B'、C'、D'之间的连线构成的夹角ZABC、ZA' B' C1、ZBAD、ZB' A1 D'为135-165 度。 [0006] A microfluidic reactor according to the present invention, the points A, B, C, D, A ', B' constituting the angle between the connecting, C ', D' ZABC, ZA 'B' C1, ZBAD, ZB 'A1 D' is 135-165 degrees.

[0007] 本发明一种微流体反应器中,所述反应池板上下表面平整、光滑;下表面有突出的定位柱与设于基座上的定位孔相吻合。 An inventive micro fluidic reactor [0007] present in the reaction cell plate surface flat and smooth; lower surface projecting positioning post and the positioning hole is provided on the base coincide.

[0008] 本发明一种微流体反应器中,设于基座上的进液孔、出液孔分别连通设于基座侧面的进液口和出液口。 [0008] A microfluidic reactor according to the present invention, inlet holes provided in the base, the liquid communication holes are provided in the sides of the base liquid inlet and a liquid outlet.

[0009] 本发明一种微流体反应器中,所述基座上设有一^^槽,卡槽中部设有一个内螺纹,所述反应池板、玻片嵌装在所述卡槽中,所述旋盖外圈有外螺纹,旋装在所述内螺纹上。 [0009] A microfluidic reactor according to the present invention, the base is provided with a groove ^^, is provided with an internally threaded central slot, said reaction cell plate, fitted in the slide slot, the outer cap has an external thread, screwed mounted on said internal thread.

[0010] 本发明一种微流体反应器中,所述旋盖中间设有可使光照自由通过的中空结构,下表面设有一个沟槽,所述橡胶圈嵌套在所述沟槽中。 [0010] A microfluidic reactor according to the present invention, the intermediate cap is provided with a hollow structure of light can pass freely, the lower surface is provided with a groove that the rubber ring is nested in the groove.

[0011] 本发明一种微流体反应器中,所述基座侧面设有电热棒和温度传感器的嵌入孔,与外设控制系统电连接的电热棒和温度传感器嵌装在所述嵌入孔中。 [0011] A microfluidic reactor according to the present invention, the base side surface is provided with an electric rod insertion holes and the temperature sensor, the heating rod and a temperature sensor connected to the control system power peripherals fitted in the fitting hole .

[0012] 本发明的工作原理简述如下: [0012] Description of the invention works as follows:

[0013] 所述基座上依次叠置有反应池板、玻片,所述旋盖旋装在所述基座上,三者构成了完整的反应室,并通过嵌装在旋盖底部的橡胶圈压装在玻片上将反应池密闭;所述反应池板中部设有一类似梭形的,无底、无盖的反应池;由于反应池在形状上,从端口到中间为一段逐渐放宽的光滑圆弧,与常规的反应池从端口到中间为一段直线相比,可以使入口处相对流速较高的流体在纵轴方向得到很好的扩散,较快的充满整个反应池,从而有利于横轴方向的流速减小而趋于均匀,很快得到流速均一分布的流场,从而是溶液在反应池中均匀推进,无残液。 [0013] The upper base are stacked successively reaction cell plate, the slide, the cap is screwed on said base, constitute three complete the reaction chamber, and at the bottom by a screw cap fitted rubber ring press fitted on slides in a sealed reaction vessel; said reaction cell plate provided with a central spindle of a similar, non-bottom, the reaction vessel without a lid; in shape due to the reaction tank, from the port to the intermediate section is gradually relaxed smooth arc, as compared to some conventional straight line from the port to the intermediate reaction tank can be made relatively higher flow rates at the inlet well fluid diffusion in the longitudinal axis, the faster it fills the reaction cell to facilitate the horizontal axis direction is reduced and the flow rate becomes uniform quickly velocity flow field uniformly distributed, so that the solution is uniformly advanced in a reaction cell, no residue. 本发明制备的反应池,流体在反应室内流速均一,流场分布均匀,使流体排出反应室时无残液。 Preparation of the reaction cell of the present invention, a uniform flow rate of the fluid in the reaction chamber, a uniform distribution of the flow field, the fluid discharged from the reaction chamber when no residue. · ·

[0014] 由于反应池板上下表面都平整、光滑,可以平整、严密的放在所述基座上,通过旋装在卡槽中间内螺纹上的旋盖使玻片、反应池板、基座,三者构成了完整的密封反应室;旋盖上表面有旋杆,旋动旋杆可以使旋盖在卡槽上水平下移,进而保证下方受压的玻片均匀受力。 [0014] Since the surfaces are smooth, can be flat, tight reaction vessel placed under the base plate, by spin within the intermediate slot on the threaded cap so that the slide, the reaction cell plate, the base , forming a complete three sealed reaction chamber; cap upper surface of rotating rod, rotating the rotating rod can be made at the slot level screwing down, thus ensuring a uniform pressure force of the lower slide. 嵌装在所述旋盖下表面沟槽中的橡胶圈,可起到缓冲作用。 Fitted in the lower surface of the trench cap rubber ring, can play the role of a buffer.

[0015] 反应池板下表面有突出的定位柱,所述基座上表面有与之相吻合的定位孔,可以实现反应池板在基座上的准确定位。 [0015] surface of a reaction cell plate projecting positioning post, said base surface coinciding with the positioning holes, the positioning accuracy can be achieved in the reaction vessel on the base plate.

[0016] 所述基座上表面具有分别连通于侧面进液口和出液口的进液孔和出液孔,其液孔位置和形状大小分别与定位在基座上的反应池的两端口相对应。 [0016] The surface having two side ports are in communication with the inlet and an inlet hole and outlet hole liquid inlet, liquid inlet position and its shape and size and are positioned on the base of the reaction cell of the base Corresponding. 基座上表面平整、光滑,构成了反应室的底,利于流体顺畅流动。 The base surface smooth, constitute the bottom of the reaction chamber, facilitate fluid flow smoothly.

[0017] 所述基座上设有卡槽,其间距与玻片相当,可以起到玻片的定位和固定作用。 With the [0017] upper slot base which slides considerable spacing, you can play the role of positioning and fixation of the slide.

[0018] 所述旋盖中间为中空结构,可以在需要光照条件的实验中使所需光路自由通过,还可以透过玻片观察反应室内的情况。 [0018] The intermediate cap has a hollow structure, may be required in the experimental manipulation of lighting conditions by a desired light path freely, the case may also be observed through the reaction chamber slides.

[0019] 所述基座侧面有电热棒或温度传感器的嵌入孔,可嵌入受电路控制的电热棒或温度传感器,通过外设控制系统实现反应室的温度控制。 [0019] The sides of the base insertion hole with an electric rod or temperature sensor, or can be embedded electric heating rods circuit controlled by the temperature sensor, the temperature of the reaction chamber is achieved by controlled peripheral control system.

[0020] 本发明的有益效果简述于下: [0020] Advantageous effects of the present invention is outlined below:

[0021] 本发明设计了一种微流体反应器,包括基座、反应池板、玻片、橡胶圈和旋盖。 [0021] The present invention contemplates a microfluidic reactor comprising a base, the reaction cell plate, slide, capping and rubber ring. 因为反应池采用了仿生学设计,端口到中间为一段逐渐放宽的光滑圆弧,使反应池整体形状为类似鱼类的梭形,由此可以使入口处相对流速较高的流体很好的向周边扩散,所以可以很快得到流速均一分布的流场,这种梭形的反应池与常规菱形反应池相比,提高了均一性。 Since the reaction cell design uses bionics, the smooth circular arc to the intermediate port is a length gradually relaxed, so that the overall shape is similar to the reaction cell spindle fish, thereby allowing the relative flow rates at the inlet to the higher fluid good surrounding diffusion can be quickly uniformly distributed velocity flow field, as compared to conventional diamond reaction vessel shuttle of this reaction cell, to improve the uniformity. 由于流场速度的均匀分布,使流体在反应池中均一推进,浓度均匀,排出时无残余。 Since uniform flow field velocity of the fluid in the reaction cell to promote uniform, uniform concentration, no residue is discharged. 所以该反应器不受放置方位影响,可水平、竖直或是按其他方位角度任意放置。 The reactor is therefore not affect the placement orientation, horizontally, vertically, or according to any other angles of orientation of placement.

[0022] 旋盖的螺纹固定方式设计使反应室密闭时受力水平均匀。 [0022] Attachment screw cap design of the reaction chamber sealed force uniform level. 旋盖的中空结构,可实现在反应室中进行光照反应并可对反应进程进行观察。 Capping of the hollow structure, the light can be realized in a reaction chamber and the reaction progress was observed. 温控功能的加入也使该反应器具有广泛的适用性。 Temperature control function is also added to the reaction having broad applicability. 同时多种定位设计使反应器整体装配简单,可广泛用于生物、化学、医药等各领域的微流体反应实验。 At the same time the entire assembly of multiple location reactor design is simple, can be widely used in various fields microfluidic biological, chemical, pharmaceutical and other response experiment.

附图说明 BRIEF DESCRIPTION

[0023] 附图1是微流体反应器分解示意图: [0023] Figure 1 is an exploded schematic view of a microfluidic reactor:

[0024] 附图2是微流体反应器的装配示意图: [0024] Figure 2 is a schematic view of the assembly of the microfluidic reactor:

[0025] 附图3是基座不意图: [0025] Figure 3 is not intended to be the base:

[0026] 附图4是反应池板示意图: [0026] Figure 4 is a schematic view of pool plate reactor:

[0027] 附图5是旋盖示意图: [0027] Figure 5 is a schematic view Capping:

[0028] 附图6是反应池形状构成示意图: [0028] Figure 6 is a schematic view of the reaction cell shape configuration:

[0029] 附图7是反应池、光斑和旋盖内缘相对位置示意图: [0029] Figure 7 is a reaction tank, and an inner edge position of the light spot schematic capping opposite:

[0030] 附图8为常规菱形反应池; [0030] Figure 8 is a conventional diamond reaction cell;

[0031] 附图9为本发明梭形反应池; [0031] BRIEF spindle 9 the reaction vessel of the present invention;

[0032] 附图10-1是常规菱形反应池的速度等值线分布云图; [0032] FIG. 10-1 is a conventional diamond velocity contour contours of the reaction cell;

[0033] 附图10-2是本发明梭形反应池的速度等值线分布云图; [0033] FIG. 10-2 is a velocity contour of the spindle of the present invention, the reaction cell contours;

[0034] 具体实施方法 [0034] DETAILED DESCRIPTION Method

[0035] 下面结合附图及及实施例对本发明作进一步详细说明: [0035] The drawings and preferred embodiments of the present invention is described in further detail below in conjunction with:

[0036] 实施例1 [0036] Example 1

[0037] 参见附图1-10,一种微流体反应器,包括:基座10、反应池板20、玻片30、橡胶圈40和旋盖50,所述反应池板20放在基座10上,所述玻片30盖在所述反应池板20上,三者构成了完整的反应室,并通过所述橡胶圈40缓冲由所述旋盖50水平压在玻片上将反应室密闭。 [0037] Referring to figures 1-10, a microfluidic reactor comprising: a base 10, a reaction cell plate 20, slide 30, the rubber ring 40 and cap 50, the reaction vessel 20 on the base plate 10, the slide cover 30 in the reaction vessel plate 20, constitute three complete the reaction chamber, and the buffer 40 by the cap 50 slides horizontally on the reaction pressure in the chamber sealed by rubber ring .

[0038] 利用PDMS通过磨具制出的反应池板20上下表面平整光滑,长60mm,宽25mm,厚Imm,下表面两个定位柱21为边长2mm的立方体,反应池板中部缺失形成的反应池24无底、无盖,形状对称,中间宽,两端窄,类似梭形。 [0038] With the PDMS smooth surface by the grinding wheel 20 made of a vertical reaction cell plate, length 60mm, width of 25mm, a thickness of Imm,, the lower surface of the two positioning posts 21 of 2mm cube side length, the middle of the reaction cell plate formed deleted no reaction cell bottom 24, without cover, symmetrical shape, the intermediate width, narrow ends, similar to the spindle. 反应池24两端为圆形端口,一个为进液孔22,另一端为出液孔23,半径均为0.9mm。 The reaction cell 24 at both ends of circular ports, one of 22, the other end of the fluid inlet bore hole 23, both the radius of 0.9mm. 所述反应池形状中心对称,中间宽,两端窄,类似梭形;所述反应池的两端与进液孔22,出液孔23轮廓相交于点C、C'、D、D',形成反应池的进液口或出液口,所述进液口或出液口的宽度CC'或DD'为1.2mm;反应池中部的轮廓线为直线AB及直线F B',直线AB或直线F B'的长度为16mm,直线AB与直线F B'之间的间距为12mm;所述直线AB或直线A' B'两端分别通过圆弧BC、AD或B ' C'、A1 D'、与反应池的进液孔22,出液孔23轮廓相交于点C、D或C'、D';所述圆弧BC、AD、B' C1、k' D'中,圆弧BC或B' C1为过点C、B、E三点或点C'、B'、E'三点并与直线AB或A' B'相切的圆弧,所述点E或E'是点C或C'以B B'为轴的对称点;圆弧AD或k' Di为过点D、A、F三点或D1、k'、F'三点并与直线AB或Ai B'相切的圆弧,所述点F或F'是点D或D'以AA'为轴的对称点。 The reaction vessel centrosymmetric shape, intermediate width, narrow ends, similar to the spindle; both ends of the reaction tank liquid inlet hole 22, outlet hole 23 contour at point C, C ', D, D', a liquid inlet or liquid outlet, said liquid inlet or liquid outlet is formed in the reaction cell width CC 'or DD' of 1.2mm; reaction cell contour central line AB and a straight line is F B ', or the line AB straight F B 'length of 16mm, lines AB and F B' is the spacing between 12mm; the straight line AB or a 'B' both ends by a circular arc BC, AD or B 'C', A1 D ', with the inlet aperture of the reaction vessel 22, outlet hole 23 contour at point C, D or C', D '; the arc BC, AD, B' C1, k 'D', a circular arc BC or B 'C1 too point C, B, E, or three o'clock point C', B ', E' three o'clock and the straight line AB or a 'B' of the arc tangent of the point E or E 'is a point C or C 'to B B' axis point of symmetry; arc AD or k 'Di too point D, a, F, or three D1, k', F 'and the straight line AB or three Ai B' phase cutting arc, the points F and F 'is a point D or D' to AA 'axis point of symmetry. 所述点A、B、C、D、A'、B'、C'、D'之间的连线构成的夹角Z ABC、Bi Ci ,Z BAD, Z Bi A' O'为145度。 The points A, B, C, D, A ', B' constituting the angle between the connecting, C ', D' Z ABC, Bi Ci, Z BAD, Z Bi A 'O' is 145 degrees. 反应池平面面积约为336mm2,体积约为0.336ml。 Plane area of ​​the reaction cell approximately 336mm2, a volume of about 0.336ml.

[0039] 所述基座10上表面有深2.2mm,边长2mm的长方体定位孔15,反应池板20可以通过定位柱21和定位孔15实现在基座10上的准确定位,基座10上表面平整光滑,有两个半径0.9mm的圆孔,一个为进液孔13,另一个为出液孔14,分别与反应池24的进液口22和出液口23对应吻合。 [0039] The upper surface of the base 10 has a depth of 2.2mm, 2mm long rectangular side positioning hole 15, the reaction cell 15 can be achieved accurately plate 20 positioned on the base 10 by a positioning post 21 and the positioning hole, the base 10 smooth upper surface, there are two circular radius of 0.9mm, a hole 13 for the inlet and one for outlet hole 14, respectively, and the reaction liquid inlet 24 of the tank 22 and a liquid outlet 23 corresponding to the anastomosis. 进液孔13、出液孔14分别连通于基座10侧面的进液口11和出液口12,进液口11和出液口12为美制10-32的螺纹孔,可通过手紧接头与外径为1/16" Inlet holes 13, 14 are communicated to the inlet port 10 side of the base 11 and the liquid inlet outlet port 12, the inlet port 11 and outlet port 12 is a threaded hole American 10-32, by manpower linker an outer diameter of 1/16 "

(1.5875mm)的teflon 管相连通。 (1.5875 mm) through a teflon tube is connected.

[0040] 基座10上有突起的卡槽17,槽间距25mm,高8mm,可以将宽25mm,长75mm,厚Imm的玻片30定位,玻片30贴盖在反应池板20上部,与反应池板20和基座10 —起构成完整 Have the [0040] slot 10 of the base 17 of the protrusion, the groove 25mm, 8mm high pitch width may be 25mm, length of 75mm, a thickness of Imm positioning slides 30, 30 slide in the upper portion of the reaction cell cover plate 20, and The reaction tank 20 and the base plate 10 - a complete since

的反应室。 The reaction chamber.

[0041 ] 反应室通过旋盖50压在玻片30上进行密闭。 [0041] The reaction chamber is sealed by a cap 30 on the slide 50 is pressed. 所述旋盖50高8mm,外围有直径34mm的外螺纹53,上表面有两个旋杆51。 The cap 50 high 8mm, 34mm diameter threaded outer periphery 53 of the upper surface 51 there are two rotating rod. 所述基座卡槽17上有与旋盖50吻合的直径34mm的内螺纹16,旋动旋杆51,可以使旋盖50水平向下压在玻片30上,玻片30受力均匀,保证了反应室的完好密闭。 The base has a slot 17 on the cap 50 coincide with the diameter of 34mm internal threads 16, rotating the rotating rod 51, so that cap 50 can be pressed down horizontally on the slide 30, the slide 30 by a uniform force, ensure the integrity of the sealed chamber.

[0042] 所述旋盖50下表面有一个圆环形沟槽52,可以嵌套内半径12.6mm,外半径15mm的橡胶圈40,起到缓冲作用。 [0042] surface of the lower cap 50 has an annular groove 52, can be nested within a radius of 12.6mm, 15mm outer radius of the rubber ring 40, cushion.

[0043] 所述旋盖50中间为中空的圆筒54,内径为20mm,在需要光照条件的实验中可使所需光路自由通过,还可以透过玻片观察反应室内的情况。 [0043] The intermediate cap 50 is a hollow cylinder 54, an inner diameter of 20mm, the experiment can require lighting conditions in the desired optical path through free, can also be observed where the reaction chamber through the slide. 如图7所示,为 As shown in FIG 7, is

14.0288mmX 10.5216mm (4:3)的光斑70与旋盖圆筒54边缘和反应池24的相对位置示意图。 14.0288mmX 10.5216mm (4: 3) is a schematic view of relative positions of the spot 70 of the cylindrical cap 54 and the edge 24 of the reaction cell.

[0044] 所述基座10侧面有电热棒嵌入孔19和温度传感器嵌入孔110,可分别嵌入受电路控制的电热棒或温度传感器,通过外设控制系统实现反应温度控制。 [0044] The surfaces of the base 10 with an electric rod fitting hole 19 and a temperature sensor insertion holes 110, respectively, may be fitted by the heating rod or temperature sensor circuit that controls the reaction temperature is achieved by controlling the peripheral control system.

[0045] 采用Fluent计算流体力学软件对本实施例的梭形反应池和参数相对应的常规菱形反应池进行建模仿真。 [0045] The Fluent CFD software and parameters of the reaction cell fusiform embodiment according to the present embodiment corresponding to the conventional rhombus model and simulate the reaction vessel. 为了简化模型,反应池的进液和出液通道用矩形代替,所述矩形通道长1.8mm,宽1.2mm,与反应池两端对应(如图8、图9所示)。 To simplify the model into the liquid, and the reaction cell was replaced by a rectangular channel, a rectangular channel length 1.8mm, width 1.2mm, corresponding to both ends of the reaction vessel (FIG. 8, FIG. 9). 仿真的入口流速边界条件为5mm/s,仿真结果的效果对比选用0.5mm高度处的中间平面,分析该平面的速度分布等值线云图(如图10-1,图10-2所示),对比参数选择均匀率R,R=A/T.其中,A代表反应区内速度在0.47-0.95mm/s之间的流场的面积,即均匀面积,T代表整个反应池的总面积。 Inlet velocity boundary conditions for the simulation contrast s, the effect of 5mm / simulation result of the selection of the median plane at the height of 0.5mm, the plane velocity distribution analysis contour plot (FIG. 10-1 and 10-2), alignment parameters selected uniform rate R, R = a / T. wherein a represents the area of ​​the flow field velocity in the reaction zone between 0.47-0.95mm / s, i.e. a uniform area, T represents the total area of ​​the entire reaction cell. 在菱形反应池中,均匀面积A为204.3mm2,总面积T为298.1mm2,均匀率R=204.3/298.1=68.53%。 Reaction cell at diamond, uniform area A 204.3mm2, T is the total area of ​​298.1mm2, uniform rate R = 204.3 / 298.1 = 68.53%.

[0046] 在梭形反应池中,均匀面积A为260.2mm2,总面积T为366.1mm2,均匀率R=260.2/336.1=77.42%。 [0046] In a reaction cell spindle, uniform area A 260.2mm2, T is the total area of ​​366.1mm2, uniform rate R = 260.2 / 336.1 = 77.42%. 可见,梭形反应池的均匀率较大,说明梭形反应池的均匀效果好于常规菱形反应池。 Visible, even greater rate of reaction cell spindle, spindle-described effect even better than a conventional reaction vessel diamond reaction cell.

[0047] 实施例2 [0047] Example 2

[0048] 实施例2的结构与实施例1相同,具体结构参数为: [0048] Structure Example 2 is similar to Example 1, specific configuration parameters:

[0049] Z ABC、ZA' B' C1、Z BAD、ZB ' A1 O1 为135 度。 [0049] Z ABC, ZA 'B' C1, Z BAD, ZB 'A1 O1 135 degrees. 所述直线AB 及直线A1 B'长2mm,直线AB及直线A' B'之间的间距为2mm。 2mm spacing between the 'long 2mm, line AB and a straight line A' B 'of the straight line AB and a straight line A1 B. 反应池进液口或出液口的宽度CC1及DD'为0.2_,矩形通道长0.3_。 The reaction tank liquid inlet or liquid outlet having a width of CC1 and DD 'of 0.2_, 0.3_ long rectangular channel. 反应池总面积T约为7mm2。 The total area of ​​the reaction tank T about 7mm2. 以入口速度5mm/s进行Fluent模拟仿真,得到的均勻速度在0.46-0.92mm/s之间的均勻面积A为5.4mm2,均匀率R (A/T)为77.14% ;对比相应的常规菱形池,反应池总面积T为6.1mm2,均匀面积A为4mm2,均匀率R为65.57%。 At an inlet speed of 5mm / s for Fluent simulation, resulting in a uniform area A uniform velocity between 0.46-0.92mm / s is 5.4mm2, uniform rate R (A / T) was 77.14%; Comparative corresponding conventional diamond pool , T is the total area of ​​the reaction cell 6.1mm2, 4mm2 area A uniformly, a uniform rate R is 65.57%. 在该实施例中,梭形反应池的均匀率较大,说明梭形池的均匀效果好于常规菱形反应池。 In this embodiment, the uniformity of the reaction cell larger spindle, spindle cell described effect even better than a conventional diamond reaction cell.

[0050] 实施例3 [0050] Example 3

[0051] 实施例3的结构与实施例1相同,具体结构参数为: [0051] Structure Example 3 is similar to Example 1, specific configuration parameters:

[0052] Z ABC、ZA' B' C'、Z BAD、ZB ' A1 O1 为165 度。 [0052] Z ABC, ZA 'B' C ', Z BAD, ZB' A1 O1 165 degrees. 所述直线AB 及直线A1 B'长60mm,直线AB及直线A' B'之间的间距为50mm,反应池进液口或出液口的宽度CC1及DD'为6_,矩形通道长9_。 The straight line AB and a straight line A1 B 'length 60mm, line AB and a straight line A' B 'is the spacing between 50mm, the reaction tank liquid inlet or liquid outlet width CC1 and DD' is 6_, 9_ long rectangular channel. 反应池总面积T约为8979mm2。 The total area of ​​the reaction tank T about 8979mm2. 以入口速度50mm/s进行Fluent模拟仿真,得到均勻速度在7.2-10.9mm/s之间的均勻面积A为6079mm2,均勻率R (A/T)为67.70% ;对比相应的常规菱形池,反应池总面积T为7706mm2,均匀面积A为4210mm2,均匀率R为54.63%。 At an inlet speed of 50mm / s for Fluent simulation, give a uniform rate evenly between the area A 7.2-10.9mm / s to 6079mm2, uniform rate R (A / T) was 67.70%; corresponding conventional diamond pool contrast, the reaction T cell total area of ​​7706mm2, uniform area A 4210mm2, uniform rate R is 54.63%. 在该实施例中,梭形反应池的均匀率较大,说明梭形池的均匀效果好于常规菱形反应池。 In this embodiment, the uniformity of the reaction cell larger spindle, spindle cell described effect even better than a conventional diamond reaction cell.

[0053] 实施例4 [0053] Example 4

[0054] 实施例4的结构与实施例1相同,具体结构参数为: [0054] Structure Example 4 is similar to Example 1, specific configuration parameters:

[0055] 反应池高1mm,所述直线AB及直线A' B'长16mm,直线AB及直线A' B'之间的间距为12mm,反应池进液口或出液口的宽度CC'及DD'为2mm,矩形通道长3mm。 [0055] High reaction vessel 1mm, the line AB and the line A spacing of 12mm between the 'B' 'B' length 16mm, line AB and a straight line A, the reaction tank liquid inlet or liquid outlet width CC 'and DD 'is 2mm, rectangular channel length 3mm. 仿真的入口流速边界条件为5mm/s, Inlet velocity boundary condition is simulated 5mm / s,

[0056] 采用Fluent计算流体力学软件对本实施例的梭形反应池和参数相对应的常规菱形反应池进行建模仿真,仿真张角Z ABC分别为125度、135度、145度时的三组模型。 [0056] The conventional computational fluid dynamics software Fluent diamond reaction cell and spindle cell reaction parameters corresponding to the present embodiment, modeling simulation, simulation Z ABC aperture angle are 125 degrees, 135 degrees, 145 degrees at the time of the three groups model. 同时为了得到梭形反应池形状的最优解,继续增大梭形池的张角ZABC到148度和152度进行建模仿真。 Meanwhile, in order to obtain optimum reaction cell spindle shape, spindle cell continues to increase the opening angle of 148 degrees and 152 ZABC of simulation modeling. 仿真结果的效果对比选用0.5mm高度处的中间平面,分析该平面的速度分布等值线云图,对比参数选择均匀率R,R=A/T.其中,A代表反应区内速度在0.9-1.4mm/s之间的流场的面积,即均匀面积,T代表整个反应池的总面积。 Comparative simulation results of the effect of selection of the median plane at the height of 0.5mm, the plane velocity distribution analysis contour plot, a uniform alignment parameters selected ratio R, R = A / T. Wherein, A represents a reaction zone at the rate from 0.9 to 1.4 area of ​​the flow field between mm / s, i.e. a uniform area, T represents the total area of ​​the entire reaction cell.

[0057] 仿真计算结果见表1,从表I可以得出:三组对比仿真中,梭形反应池的R值大于相应的菱形反应池的R值,说明本发明的梭形反应池的均匀效果优于常规菱形反应池:梭形池的张角Z ABC从125度到152度时,R值先增大后减小,到145度时最大。 [0057] The simulation results in Table 1, can be drawn from Table I: Three aging simulation, the R value is greater than the corresponding spindle reaction vessel R value rhombic reaction cell, the reaction cell described uniform spindle invention better than conventional diamond reaction cell: cell opening angle Z ABC spindle 125 degrees to 152 degrees from, the R value increases and then decreases, when the maximum 145 °. 进一步增大张角,均匀面积的值增加接近饱和,但是总面积的增加使R值减小,也浪费了反应试剂,本实施例中,145度为最优解。 Opening angle is further increased, the value of a uniform increase in the area close to saturation, but the increase in the total area of ​​the R value is reduced, but also a waste of reactant, in this embodiment, 145 degrees is the optimal solution.

[0058] 表I为常规菱形反应池与本发明梭形反应池fluent仿真后得到的数据统计。 [0058] Table I is a conventional reaction vessel with a diamond spindle reaction of the present invention obtained after the simulation statistics pool fluent.

[0059]表 I [0059] TABLE I

Figure CN102698672BD00081

[0061] 以上所述实施例仅表达了本发明的实施方式,其描述较为具体和详细,但并不能因此而理解为本发明专利范围的限制。 [0061] The above embodiments are only expressed embodiment of the present invention, and detailed description thereof is more specific, but can not therefore be understood to limit the scope of the present invention. 应当指出的是,对于本领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干变形和改进,这些都属于本发明的保护范围。 It should be noted that those of ordinary skill in the art, without departing from the spirit of the present invention, can make various changes and modifications, which fall within the protection scope of the present invention. 因此,本发明专利的保护范围应以所附权利要求为准。 Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (7)

1.一种微流体反应器,包括:基座、反应池板、玻片、橡胶圈和旋盖,所述基座上依次叠置有反应池板、玻片,所述旋盖旋装在所述基座上并通过所述橡胶圈压装在所述玻片上,其特征在于:所述反应池板中部设有一类似梭形的反应池,所述反应池长度方向的两端各设有一个进液孔、出液孔,所述进液孔、出液孔与设于基座上的进液通道或出液通道相对应;所述反应池形状中心对称,中间宽,两端窄,类似梭形;所述反应池的两端与进液孔、出液孔轮廓相交于点C、C1、D、D',形成反应池的进液口或出液口,所述进液口或出液口的宽度CC'或DD'为:0.2-6mm;反应池中部的轮廓线为直线AB及直线A' B',直线AB或直线A1 B'的长度为2-60mm,直线AB与直线V B'之间的间距为2_60mm ;所述直线AB或直线A1 Bi两端分别通过圆弧BC、AD或K C1、k' D1、与反应池的端点C、D或连接;所述圆弧B 1. A microfluidic reactor comprising: a base, the reaction cell plate, slides, rings and rubber capping, the reactions are stacked successively on the base pool plate, slide, the cap is screwed on said base pressure by the rubber ring mounted on said slide, characterized in that: said reaction vessel is provided with a plate similar to the central spindle of the reaction cell, said reaction cell at both ends of longitudinal direction of each is provided a liquid inlet hole, a liquid inlet, a liquid inlet hole, corresponding to the outlet hole provided in the inlet passage or the fluid passage of the base; said reaction vessel centrosymmetric shape, intermediate width, narrow ends, Similarly spindle; the two ends with the inlet aperture pool reaction, the liquid inlet contour at point C, C1, D, D ', forming a liquid inlet or liquid outlet of the reaction tank, or the inlet opening liquid outlet width CC 'or DD' is: 0.2-6mm; reaction cell contour central line AB and a straight line is a 'B', the length of straight line AB or A1 B 'is 2-60mm, lines AB and V B 'is the spacing between 2_60mm; A1 both ends of the line AB or Bi respectively straight arc BC, AD or K C1, k' D1, and the endpoint of the reaction cell C, D or; said arc B C、AD、B' Ci ,A1 Di中,圆弧BC或K Ci为过点C、B、E三点或点(T ,B1、Ei三点并与直线AB或A' B'相切的圆弧,所述点E或E'是点C或C'以BB'为轴的对称点;圆弧AD或A' D'为过点D、A、F三点或D'、A'、F'三点并与直线AB或A' B'相切的圆弧,所述点F或F'是点D或D'以AA'为轴的对称点。 C, AD, B 'Ci, A1 Di, the arc BC or through point K Ci to C, B, E, or three o'clock point (T, B1, Ei three o'clock and the straight line AB or A' B 'tangent arc, the point E or E 'is a point C or C' to BB 'axis point of symmetry; arc AD or a' D 'is the point through D, a, F three- or D', a ', F 'three o'clock and the straight line AB or a' B 'of the arc tangent of the point F and F' is a point D or D 'to AA' axis point of symmetry.
2.根据权利要求1所述的一种微流体反应器,其特征在于:所述点A、B、C、D、A'、B'、C1、D'之间的连线构成的夹角ZABC、ZA' B' C1、ZBAD、ZB' A1 D'为135-165 度。 2. A microfluidic reactor of claim 1, wherein wherein: the point A, B, C, D, A ', B' between the connecting angle, C1, D 'consisting of ZABC, ZA 'B' C1, ZBAD, ZB 'A1 D' is 135-165 degrees.
3.根据权利要求2所述的一种微流体反应器,其特征在于:所述反应池板上下表面平整、光滑;下表面有突出的定位柱与设于基座上的定位孔相吻合。 3. A microfluidic reactor according to claim 2, wherein: said reaction cell plate surface flat and smooth; lower surface projecting positioning post and the positioning hole is provided on the base coincide.
4.根据权利要求3所述的一种微流体反应器,其特征在于:设于基座上的进液孔、出液孔分别连通设于基座侧面的进液口和出液口。 4. A microfluidic reactor according to claim 3, characterized in that: the inlet holes provided in the base, the liquid communication holes are provided in the sides of the base liquid inlet and a liquid outlet.
5.根据权利要求4所述的一种微流体反应器,其特征在于:所述基座上设有一卡槽,卡槽中部设有一个内螺纹,所述反应池板、玻片嵌装在所述卡槽中,所述旋盖外圈有外螺纹,旋装在所述内螺纹上。 5. A microfluidic reactor according to claim 4, wherein: said base is provided with a slot, the slot is provided with a central internal thread, said reaction cell plate, slide fitted the slot, the outer cap has an external thread, screwed mounted on said internal thread.
6.根据权利要求5所述的一种微流体反应器,其特征在于:所述旋盖中间设有可使光照自由通过的中空结构,下表面设有一个沟槽,所述橡胶圈嵌套在所述沟槽中。 6. A microfluidic reactor according to claim 5, wherein: said intermediate cap is provided with a hollow structure of light can pass freely, the lower surface is provided with a groove, a rubber ring nested in the groove.
7.根据权利要求6所述的一种微流体反应器,其特征在于:所述基座侧面设有电热棒或温度传感器的嵌入孔,与外设控制系统电连接的电热棒或温度传感器嵌装在所述嵌入孔中。 7. A microfluidic reactor according to claim 6, wherein: the base is provided with a fitting hole side temperature sensor or electric heating rods, heating rod or temperature sensor is electrically connected to the peripheral control system embedded mounted in said insert hole.
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