ELISA Plate with absorption affinity
Technical field
The utility model relates to a kind of ELISA Plate, particularly relates to the ELISA Plate that a kind of inwall has absorption affinity.
Background technology
ELISA Plate (ELISAPLATE) be enzyme linked immunosorbent assay (Enzyme LinkedImmuno-sorbent Assay, ELISA) in important carrier instrument.It has important effect in immunological response.The purity of the antigen of immunological response, antibody, labelled antibody or antigen, concentration and ratio, damping fluid kind, concentration and ionic strength, pH value and conditions such as temperature of reaction, time are all in close relations with ELISA Plate.In addition, surperficial absorption to antigen, antibody or antigen antibody complex also plays an important role as the solid phase material polystyrene (Polystyrene) of carrier.Antigen, antibody and other biomolecule are adsorbed to carrier surface by number of mechanisms.
The ELISA Plate that is used for the immunoassay experiment is the structure of a kind of inside surface without any processing, and the adsorbed sample (being commonly referred to the bag quilt) in the sample cup in this kind of enzyme target (being commonly referred to microwell plate in this area) surface is generally 0.1~0.125ml as the adsorbance (or amount of title envelope antigen, antibody or mark) to antigen, antibody or mark.Because the restriction of inside surface surface area makes that the amount of reactant absorption is very limited.In immunoassay experiment, because that the absorption quantitative limitation causes some immunoreactive susceptibility is lower.Formerly immune response is tested the not high phenomenon of susceptibility that place often runs at each in the technology, and one of its important reasons is exactly to come from the lower problem of the adsorbance of sample cup in the ELISA Plate.
Summary of the invention
The purpose of this utility model is to make physics on the inside surface in the sample cup in ELISA Plate to handle (except the bottom), and it is uneven that inside surface is presented, and is processed into the shape of solid geometry, thereby the surface area of inside surface is increased.By increasing the surface area of sample cup bag quilt in the ELISA Plate, make the increase of the encrusting substance of absorption, thereby improve the susceptibility of ELISA Plate.
To achieve the above object, the technical measures taked of the utility model are: the surface area that increases the sample cup inside surface.Promptly comprise housing, be divided in housing empty every elastic baffle and central dividing plate and place emptyly every interior sample cup, make the inside surface of sample cup become rough.
Benefit of the present utility model is significant.For onesize sample cup, the utility model obviously is the surface area that has increased inside surface because inside surface is rough.When filling with sample in the sample cup, the amount of the sample that the sample of big surface area absorption is more adsorbed than little surface area is big.The amount of absorption (or claiming package amount) is big more, and the susceptibility of measurement is just strong more.And can relatively reduce the concentration and the consumption of sample.
For advantage of the present utility model more clearly is described, be that example illustrates only with hepatitis b virus s antigen diagnostic kit common in the immune response.Each 10 example of hepatitis b virus s antigen feminine gender, the positive and quality controlled serum sample of choosing respectively under the similarity condition experimentize, and draw following result.Represent with table one, two:
Table one: (in the table: ELISA Plate of the present utility model is called the S plate, and formerly the ELISA Plate of technology is called the N plate, embodiment n=10 example)
The ELISA Plate type | The negative average OD value of sample (absorbance) | The average OD value of positive sample (absorbance) |
The S plate | <0.020 | 2.95 |
The N plate | <0.020 | 2.50 |
Table two: the quality controlled serum sample with variable concentrations experimentizes, and test S plate and the average OD value (embodiment n=10 example) of N plate under similarity condition draw following result:
The ELISA Plate type | 4ng/ml OD value (absorbance) | 2ng/ml OD value (absorbance) | Ing/ml OD value (absorbance) | 0.5ng/ml OD value (absorbance) |
The S plate | 1.8 | 0.95 | 0.42 | 0.20 |
The N plate | 1.5 | 0.77 | 0.35 | 0.15 |
Test result shows: the testing result to identical sensitivity quality controlled serum shows: all greater than the mean light absorbency of N plate, in immune response was analyzed, such result was considered as susceptibility and improves the mean light absorbency of S plate.The susceptibility ratio of the utility model S plate formerly N plate of technology has improved 20.0~33.3%.
Description of drawings
Fig. 1-the 1st the utlity model has the structural representation of the ELISA Plate of absorption affinity.
Fig. 1-2 is the cut-open view of A-A face among Fig. 1-1.
Fig. 2-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into irregular particle shape.
Fig. 2-the 2nd, A-A face cut-open view among Fig. 2-1.
Fig. 2-the 3rd, the vertical view of Fig. 2-1.
Fig. 2-the 4th, the enlarged drawing of the M part among Fig. 2-2.
Fig. 3-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into the axial shape of circular arc.
Fig. 3-the 2nd, A-A face cut-open view among Fig. 3-1.
Fig. 3-the 3rd, the cut-open view of B-B face among Fig. 3-1.
Fig. 3-the 4th, the enlarged drawing of the M part among Fig. 3-3.
Fig. 4-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into arc spiral shape.
Fig. 4-the 2nd, A-A face cut-open view among Fig. 4-1.
Fig. 4-the 3rd, the vertical view among Fig. 4-1.
Fig. 4-the 4th, the enlarged drawing of the M part among Fig. 4-2.
Fig. 5-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into circular arc ring-type shape.
Fig. 5-the 2nd, A-A face cut-open view among Fig. 5-1.
Fig. 5-the 3rd, the vertical view among Fig. 5-1.
Fig. 5-the 4th, the enlarged drawing of the M part among Fig. 5-2.
Fig. 6-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into trapezoidal axial shape.
Fig. 6-the 2nd, A-A face cut-open view among Fig. 6-1.
Fig. 6-the 3rd, B-B face cut-open view among Fig. 6-1.
Fig. 6-the 4th, the enlarged drawing of the M part among Fig. 6-3.
Fig. 7-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into trapezoid spiral shape.
Fig. 7-the 2nd, A-A face cut-open view among Fig. 7-1.
Fig. 7-the 3rd, the vertical view among Fig. 7-1.
Fig. 7-the 4th, the enlarged drawing of the M part among Fig. 7-2.
Fig. 8-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into trapezoidal ring-type shape.
Fig. 8-the 2nd, A-A face cut-open view among Fig. 8-1.
Fig. 8-the 3rd, the vertical view among Fig. 8-1.
Fig. 8-the 4th, the enlarged drawing of the M part among Fig. 8-2.
Fig. 9-the 1st, the synoptic diagram of the processed rectangular shaft synform of sample cup 3 rough inside surfaces.
Fig. 9-the 2nd, A-A face cut-open view among Fig. 9-1.
Fig. 9-the 3rd, B-B face cut-open view among Fig. 9-1.
Fig. 9-the 4th, the enlarged drawing of the M part among Fig. 9-3.
Figure 10-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into rectangular coil shape.
Figure 10-the 2nd, A-A face cut-open view among Figure 10-1.
Figure 10-the 3rd, the vertical view among Figure 10-1.
Figure 10-the 4th, the enlarged drawing of the M part among Figure 10-2.
Figure 11-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into rectangular ring shape.
Figure 11-the 2nd, A-A face cut-open view among Figure 11-1.
Figure 11-the 3rd, the vertical view among Figure 11-1.
Figure 11-the 4th, the enlarged drawing of the M part among Figure 11-2.
Figure 12-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into the axial shape of triangle.
Figure 12-the 2nd, A-A face cut-open view among Figure 12-1.
Figure 12-the 3rd, B-B face cut-open view among Figure 12-1.
Figure 12-the 4th, the enlarged drawing of the M part among Figure 12-3.
Figure 13-the 1st, sample cup 3 rough inside surfaces are processed into the spiral synoptic diagram of triangle.
Figure 13-the 2nd, A-A face cut-open view among Figure 13-1.
Figure 13-the 3rd, the vertical view among Figure 13-1.
Figure 13-the 4th, the enlarged drawing of the M part among Figure 13-2.
Figure 14-the 1st, the synoptic diagram that sample cup 3 rough inside surfaces are processed into triangle ring-type shape.
Figure 14-the 2nd, A-A face cut-open view among Figure 14-1.
Figure 14-the 3rd, the vertical view among Figure 14-1.
Figure 14-the 4th, the enlarged drawing of the M part among Figure 14-2.
Embodiment
Following structure accompanying drawing further specifies the structure of the ELISA Plate that the utlity model has absorption affinity.
Shown in Fig. 1-1, Fig. 1-2 is the structure that the utlity model has the ELISA Plate of absorption affinity.Comprise: housing 1, be divided in the housing 1 empty every elastic baffle 2 and central dividing plate 4, place empty every interior sample cup 3.Sample places in the sample cup 3.Wherein elastic baffle 2 has elasticity, is convenient to the insertion of sample cup 3.The inside surface of the utility model sample cup 3 is rough.In the present embodiment: it is improved polystyrene that the material of formation housing 1, elastic baffle 2 and central dividing plate 4 is all selected HIPS-for use, and it is constant that its chemical property still belongs to polystyrene, but toughness is more intense.Constitute the pure polystyrene of material selection of sample cup 3.
Shown in Fig. 2-1, Fig. 2-2, Fig. 2-3, Fig. 2-4 is that sample cup 3 rough inside surfaces are processed into irregular particle shape, is commonly referred to irregular particle type ELISA Plate.
Shown in Fig. 3-1, Fig. 3-2, Fig. 3-3, Fig. 3-4 is that sample cup 3 rough inside surfaces are processed into the axial shape of circular arc, is commonly referred to circular arc axialmode ELISA Plate.
Shown in Fig. 4-1, Fig. 4-2, Fig. 4-3, Fig. 4-4 is that sample cup 3 rough inside surfaces are processed into arc spiral shape, is commonly referred to arc spiral type ELISA Plate.
Shown in Fig. 5-1, Fig. 5-2, Fig. 5-3, Fig. 5-4 is that sample cup 3 rough inside surfaces are processed into circular arc annular shape, is commonly referred to circular arc ring-type type ELISA Plate.
Shown in Fig. 6-1, Fig. 6-2, Fig. 6-3, Fig. 6-4 is that sample cup 3 rough inside surfaces are processed into trapezoidal axial shape, is commonly referred to trapezoidal axialmode ELISA Plate.
Shown in Fig. 7-1, Fig. 7-2, Fig. 7-3, Fig. 7-4 is that sample cup 3 rough inside surfaces are processed into trapezoid spiral shape, is commonly referred to trapezoid spiral type ELISA Plate.
Shown in Fig. 8-1, Fig. 8-2, Fig. 8-3, Fig. 8-4 is that sample cup 3 rough inside surfaces are processed into trapezoidal ring-type shape, is commonly referred to trapezoidal ring-type type ELISA Plate.
Shown in Fig. 9-1, Fig. 9-2, Fig. 9-3, Fig. 9-4 is that sample cup 3 rough inside surfaces are processed into the rectangular shaft synform, is commonly referred to rectangle axialmode ELISA Plate.
Sample cup 3 rough inside surfaces shown in Figure 10-1, Figure 10-2, Figure 10-3, Figure 10-4 are processed into rectangular coil shape, are commonly referred to rectangular coil type ELISA Plate.
Sample cup 3 rough inside surfaces shown in Figure 11-1, Figure 11-2, Figure 11-3, Figure 11-4 are processed into rectangular ring shape, are commonly referred to rectangular ring type ELISA Plate.
Sample cup 3 rough inside surfaces shown in Figure 12-1, Figure 12-2, Figure 12-3, Figure 12-4 are processed into the axial shape of triangle, are commonly referred to triangle axialmode ELISA Plate.
Sample cup 3 rough inside surfaces shown in Figure 13-1, Figure 13-2, Figure 13-3, Figure 13-4 are processed into the triangle spirality, are commonly referred to triangle screw type ELISA Plate.
Sample cup 3 rough inside surfaces shown in Figure 14-1, Figure 14-2, Figure 14-3, Figure 14-4 are processed into triangle ring-type shape, are commonly referred to triangle ring-type type ELISA Plate.