CN104977405B - Immunodetection micropore - Google Patents
Immunodetection micropore Download PDFInfo
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- CN104977405B CN104977405B CN201510408362.9A CN201510408362A CN104977405B CN 104977405 B CN104977405 B CN 104977405B CN 201510408362 A CN201510408362 A CN 201510408362A CN 104977405 B CN104977405 B CN 104977405B
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- 238000001514 detection method Methods 0.000 claims abstract description 60
- 230000005494 condensation Effects 0.000 claims description 10
- 238000009833 condensation Methods 0.000 claims description 10
- 230000011514 reflex Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 51
- 238000006243 chemical reaction Methods 0.000 abstract description 27
- 230000001965 increasing effect Effects 0.000 abstract description 21
- 230000035945 sensitivity Effects 0.000 abstract description 16
- 239000000523 sample Substances 0.000 abstract description 4
- 238000006555 catalytic reaction Methods 0.000 abstract description 2
- 230000036046 immunoreaction Effects 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 15
- 238000001179 sorption measurement Methods 0.000 description 14
- 230000003287 optical effect Effects 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 238000004020 luminiscence type Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 3
- 239000000376 reactant Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000003851 corona treatment Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses an immunodetection micropore, which comprises a micropore cavity. The micropore cavity is connected by a round through-hole at the upper part of the micropore cavity and a cup hole, provided with an annular side wall which can increase an upwards projected and reflected concentrated light area, at the lower part of the micropore cavity. A reflector which can increase the upwards projected and reflected concentrated light area is projected from the bottom and/or the annular side wall of the cup hole. According to the cup hole at the lower part of the micropore cavity, an interfacial area of a solid-liquid reaction of a detected liquid is increased, so that an immunoreaction, a luminous reaction, or an enzymic catalytic reaction can be fully carried out, and the detection sensitivity is improved; and at the same time, the upwards projected and reflected concentrated light area of the interfacial area of the solid-liquid reaction is increased, so that the interfacial area of the solid-liquid reaction can be effectively illuminated by projected light, and can reflect and converge the light; a photomultiplier tube (PMT) probe of a detection device can receive more light signals from the interfacial area of the solid-liquid reaction; and the detection sensitivity is greatly improved.
Description
Technical field
The present invention relates to a kind of micropore of immune detection, specifically one kind carry in bioscience research or medical diagnosiss
The microcellular structure of the microwell plate of high immune detection reaction rate and sensitivity.
Background technology
In life sciences, medical research and medical diagnosiss activity, the elisa based on microwell plate and change
Learn being widely used of electrochemiluminescent immunoassay detection test.For improving immune detection reaction rate and sensitivity, general method is to adopt
Surface treatment, the such as mode such as r roentgenization, ultraviolet irradiation, corona treatment is to strengthen its absorbability, but improves journey
Degree is limited.In addition, the response area of reactant liquor solid liquid interface can be made to increase by increasing the surface area of the micropore inner chamber of microwell plate
Plus, improve detection sensitivity, shorten detection time.Although passing through recessed in micropore in the microcellular structure of existing microwell plate (bar)
Convex process can increase solid and liquid interface (adsorption area of test solution) with improve adsorption plane but for luminous detection, existing
During microcellular structure fails to make electrochemiluminescent immunoassay detect, solid liquid interface light is thrown according to effective area increase, leads to detecting instrument to detect
The signal intensity of the reflected light at solid and liquid interface is weaker, and therefore detection sensitivity is low.
Content of the invention
It is contemplated that proposing a kind of micropore of immune detection, both increasing immune detection solid and liquid interfacial area, having increased again
Plus to upslide according to the effective illuminated area with reflecting condensation, so that the reaction rate of adsorption liquid immune detection and sensitivity is significantly carried
High.
For reaching above-mentioned purpose, the present invention provides a kind of micropore of immune detection, and including a micropore vestibule, its feature exists
In: described micropore vestibule is had from top one round tube hole and bottom one to be increased to upslide according to the side ring wall with reflecting condensation area
Cup aperture connect and compose, described cup aperture is inversed taper platform hole, and cup aperture bottom surface has increase light to upslide integrally formed with one
According to the side ring surface reflexing to cup aperture on refractive body.
The side wall of the domain in inversed taper platform hole from cup aperture mouth to cup aperture bottom, centroclinal dip, with respect to traditional vertical cyclindrical shape micropore,
The setting of cone machine hole had both increased immune detection solid and liquid interfacial area, and increasing light projects the effective illuminated face at solid and liquid interface
Long-pending.
Described inversed taper platform hole is rotation body opening, described rotation body opening is inverted round stage hole, flared hole, the hole of falling spherical zone or
Rounding halfpace hole；The whole ring wall of the cup aperture of rotary body cellular type is cambered surface, and this cambered surface is used for increasing immune detection solid and liquid interface
Area, but increasing light is projected to the effective illuminated area of photomultiplier tube upwards.
Described inversed taper platform hole is chamfered edge platform hole；The whole ring wall of cup aperture of chamfered edge platform cellular type is made up of the faceted pebble of whole circle, to
Under intilted faceted pebble be used for increasing immune detection solid and liquid interfacial area and increasing light is projected to photomultiplier tube upwards
Effective illuminated area.
Described refractive body is a segment, spherical zone body, circular cone, round platform, pyramid or terrace with edge；Refractive body due to cup aperture bottom surface
Protrude, so that immune detection liquid and the solid liquid interface area of cup aperture bottom surface is increased, having of solid and liquid interface can be projected by increasing light again
Effect irradiated area, and project the light of refractive body through in its scattered reflection to the side ring surface of cup aperture, entering an increase step cup aperture
Side ring surface optically focused intensity.
After technique scheme, solid-liquid circle of the cup aperture of immunoreation liquid and luminescence-producing reaction liquid and micropore vestibule bottom
Face area increases, and so that the response area of reactant liquor solid liquid interface is greatly increased, and is conducive to immunoreation, luminescence-producing reaction fully, soon
Speed completes, and shortens the response time；The technical scheme being formed can make the light of luminescence-producing reaction projection gather and shine detection to upslide
Photomultiplier tube (pmt) probe of instrument, makes detecting instrument the luminous signal intensity enhancing at solid and liquid interface is detected to improve
Detection sensitivity.
Beneficial effects of the present invention are as follows:
1. the cup aperture side ring wall of micropore vestibule bottom with detection liquid solid-liquid reaction interface increase, be beneficial to immunoreation,
Luminescence-producing reaction or enzymic catalytic reaction are fully carried out, and the time that reaction reaches balance greatly shortens, thus improving detection sensitivity, carries
High detection efficiency；
2. the cup aperture side ring wall of micropore vestibule bottom and the ramp structure at the solid-liquid reaction interface of detection liquid can make generation
Light-emitting line effectively converges, and so that detecting instrument photomultiplier tube (pmt) is popped one's head in and receives the optical signal of more solid liquid interfaces,
To improve detection sensitivity；
3. the refractive body of the cup aperture bottom surface of micropore vestibule bottom or/and side ring surface setting protrusion, refractive body increases solid-liquid
The solid-liquid reaction interface of vestibule bottom face or/and side, and the light effective illuminated that solid-liquid reaction interface can be projected is to simultaneously instead
Penetrate convergence, so that the optical signal that detecting instrument photomultiplier tube (pmt) probe detection arrives is increased, greatly improve the sensitivity of detection.
Brief description
Fig. 1 is the structural representation of embodiment one present invention.
Fig. 2 is the structural representation of embodiment two present invention.
Fig. 3 is the structural representation of embodiment three present invention.
Fig. 4 is the structural representation of the example IV present invention.
Fig. 5 is the structural representation of embodiment five present invention.
Fig. 6 is the structural representation of embodiment six present invention.
Fig. 7 is the structural representation of embodiment seven present invention.
Fig. 8 is the structural representation of embodiment eight present invention.
Fig. 9 is the structural representation of embodiment nine present invention.
Figure 10 is the structural representation of embodiment ten present invention.
Figure 11 is the structural representation of embodiment 11 present invention.
Figure 12 is the structural representation of embodiment 12 present invention.
Figure 13 is the structural representation of embodiment 13 present invention.
In figure accompanying drawing is designated: 10. micropore vestibule；11. round tube holes；12. inverted round stage holes；13. chamfered edge platform holes；14. loudspeaker
Shape hole；15. holes of falling spherical zone；16. rounding halfpace holes；20. refractive bodies；21. segments；22. circular cones；23. round platforms；24. pyramids；25.
Terrace with edge；26. tetrahedrons.
Specific embodiment
The present invention is further described with reference to the accompanying drawings and examples.
Embodiment one (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage bottom hole face arranges a segment).
A kind of micropore of immune detection as shown in Figure 1, including a micropore vestibule 10, micropore vestibule 10 is justified by top one
The inverted round stage hole 12 of the side ring wall that through hole 11 and bottom one have reflecting condensation upwards connects and composes, the inverted round stage hole 12 of the present invention
Bottom surface be provided with the refractive body 20 that a light reflects upwards with protruding above, the refractive body 20 of the present invention adopts segment 21 one
Molding is connected on the bottom surface in inverted round stage hole 12.
Operation principle the following detailed description of the present embodiment present invention and effect.
As shown in figure 1, immune detection liquid is injected in micropore vestibule 10 by round tube hole 11, micropore vestibule 10 bottom fall
Round platform hole 12 is filled up by immune detection liquid, compared with traditional micropore, the inverted round stage hole 12 side ring wall of the present invention and segment 21
The reaction surface area that surface is adsorbed with immune detection liquid greatly increases, and so that immunoreation is completed in a relatively short time, and improves inspection
Survey sensitivity；And the light through round tube hole 11 projection is irradiated to the side ring surface in inverted round stage hole 12 and the surface of segment 21 completely,
The side ring surface in inverted round stage hole 12 has reflecting condensation function to light, and the surface of segment 21 has light line reflection is diffused into circle
Function on the side ring surface in platform hole 12 is so that the light that the solid liquid interface reflection through inverted round stage hole 12 side ring wall and segment 21 collects is believed
Number intensity increases so that the luminous signal of reactant is higher, focus more on light-emitting appearance probe window position, to improve electrochemiluminescent immunoassay
The sensitivity of detection, shortening detection time, improve detection efficiency.
Also a facet can be cut out in segment 21 top in the present embodiment and form spherical zone body, its function and effect and segment 21
Equivalent.
Embodiment two (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage bottom hole face arranges a circular cone).
As shown in Fig. 2 the present embodiment is with the difference of embodiment one: inverted round stage hole 12 bottom surface of micropore vestibule 10 bottom
It is integrally formed connection one circular cone 22 as refractive body 20, circular cone 22 surface can reflex to inverted round stage by light thereon projection
On the side ring wall in hole 12, by the side ring wall in inverted round stage hole 12, reflected light is converged to increase the intensity of optical signal；Rounding
The conical surface of platform hole 12 ring wall and circular cone 22 had both been increased micropore vestibule 10 and had also been increased with the adsorption reaction surface area of immune detection liquid
The effective illuminated area of luminous detection, makes the response time of immune detection liquid shorten and improve detection sensitivity；The present embodiment
Other structures and operation principle with embodiment one.
Embodiment three (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage bottom hole face arranges a round platform).
As shown in figure 3, the present embodiment is with the difference of embodiment one: inverted round stage hole 12 bottom surface of micropore vestibule 10 bottom
It is integrally formed connection one round platform 23 as refractive body 20, round platform 23 side considerably increases micropore vestibule 10 bottom surface and immunity inspection
Survey absorption and the response surface surface of liquid；And by round tube hole 11 projection downwards light through round platform 23 offside reflection to inverted round stage hole
12 sides, through inverted round stage hole 12 side optically focused to increase the intensity of optical signal；The other structures of the present embodiment and operation principle are same
Embodiment one.
Example IV (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage bottom hole face arranges a pyramid).
As shown in figure 4, the present embodiment is with the difference of embodiment one: inverted round stage hole 12 bottom surface of micropore vestibule 10 bottom
Be integrally formed connection one pyramid 24 as refractive body 20, the faceted pebble of pyramid 24 surrounding considerably increase micropore vestibule 10 bottom surface with
The absorption of immune detection liquid and response surface surface, and reflexed to down through pyramid 24 surface by the light of 11 projections downwards of round tube hole
Round platform hole 12 side, through inverted round stage hole 12 side optically focused to increase the intensity of optical signal；The other structures of the present embodiment and work
Principle is with embodiment one.
Embodiment five (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage bottom hole face arranges a terrace with edge).
As shown in figure 5, the present embodiment is with the difference of embodiment one: inverted round stage hole 12 bottom surface of micropore vestibule 10 bottom
It is integrally formed connection one terrace with edge 25 as refractive body 20, terrace with edge 25 side considerably increases micropore vestibule 10 bottom surface and immunity inspection
Survey the adsorption surface area of liquid, also make by 11 light projecting downwards of round tube hole through terrace with edge 25 offside reflection to inverted round stage hole 12 side
Face, through inverted round stage hole 12 side optically focused to increase the intensity of optical signal；The other structures of the present embodiment and operation principle are with enforcement
Example one.
Embodiment six (micropore vestibule is made up of round tube hole and inverted round stage hole, and inverted round stage hole side arranges some tetrahedrons).
As shown in fig. 6, the present embodiment is with the difference of embodiment one: inverted round stage hole 12 side ring of micropore vestibule 10 bottom
Face is integrally formed some tetrahedrons 26 of connection as refractive body 20, increases itself and immune detection liquid by tetrahedron 26 two sides
Absorption and reaction surface area, by 11 light projecting downwards of round tube hole through direct irradiation inverted round stage hole 12 side ring surface and tetrahedron
26 offside reflection optically focused, that is, micropore vestibule 10 bottom inverted round stage hole 12 and the adsorption area of immune detection liquid greatly increase, and
And the adsorption area of inverted round stage hole 12 and immune detection liquid be irradiated by light can optically focused and increase detected light signal strength, with big
Big raising detection sensitivity, improves detection efficiency, the other structures of the present embodiment and operation principle are with embodiment one.
Embodiment seven (micropore vestibule is made up of round tube hole and chamfered edge platform hole, and chamfered edge platform bottom hole face arranges a segment).
As shown in fig. 7, the present embodiment is with the difference of embodiment one: micropore vestibule 10 is by round tube hole 11 and chamfered edge platform hole
13 compositions, are substituted for chamfered edge platform hole 13, chamfered edge platform hole 12 the inverted round stage hole 12 of micropore vestibule 10 bottom in embodiment one
Bottom surface is still arranged as implemented the segment 21 in；Chamfered edge platform hole 13 connects and composes a domain by the straight faceted pebble of some inclinations
Face, makes chamfered edge platform hole 13 inwall and the adsorption reaction surface of immune detection liquid greatly increase, segment 21 also increased micropore vestibule
10 bottom surfaces and the adsorption reaction surface of immune detection liquid, and the side ring surface in chamfered edge platform hole 13 and the surface of segment 21 are light inspection
Survey has an effective irradiation surface, the light on segment 21 surface can side ring surface reflecting condensation through chamfered edge platform hole 13 to strengthen luminous signal
Intensity；The operation principle of the other structures of the present embodiment is with embodiment one.
Embodiment eight (micropore vestibule is made up of round tube hole and chamfered edge platform hole, and chamfered edge platform bottom hole face arranges a circular cone).
As shown in figure 8, the present embodiment one is with the difference of embodiment seven: chamfered edge platform hole 13 bottom of micropore vestibule 10 bottom
The refractive body 20 in face is substituted for a circular cone 22, and circular cone 22 surface can reflex to the light projecting thereon the side in chamfered edge platform hole 13
On ring wall, the side ring wall by chamfered edge platform hole 13 is converged to reflected light to increase the intensity of optical signal；Chamfered edge platform hole 13 ring
The conical surface of wall and circular cone 22 had both increased micropore vestibule 10 and had also increased luminous inspection with the adsorption reaction surface area of immune detection liquid
The effective illuminated area surveyed, makes the response time of immune detection liquid shorten and improve detection sensitivity.Other knots of the present embodiment
Structure and operation principle are with embodiment seven.
The circular cone 22 of the present embodiment also equivalent can become the round platform 23 in embodiment three.
Embodiment nine (micropore vestibule is made up of round tube hole and chamfered edge platform hole, and chamfered edge platform bottom hole face arranges a pyramid).
As shown in figure 9, the present embodiment one is with the difference of embodiment seven: chamfered edge platform hole 13 bottom of micropore vestibule 10 bottom
The refractive body 20 in face is substituted for a pyramid 24, and the faceted pebble of pyramid 24 surrounding considerably increases micropore vestibule 10 bottom surface and immune detection
The absorption of liquid and response surface surface, and chamfered edge platform hole 13 is reflexed to through pyramid 24 surface by the light of 11 projections downwards of round tube hole
Side, through chamfered edge platform hole 13 side optically focused to increase the intensity of optical signal；The other structures of the present embodiment and operation principle are with real
Apply example seven.
In the present embodiment, micropore vestibule 10 bottom pyramid also equivalent can become a terrace with edge 25 as refractive body 20, its function
It is equal to this enforcement with effect.
Embodiment ten (micropore vestibule is made up of round tube hole and chamfered edge platform hole, and chamfered edge platform hole side arranges some tetrahedrons).
As shown in Figure 10, the present embodiment and the difference of embodiment six are: micropore vestibule 10 is by round tube hole 11 and chamfered edge platform
Hole 13 is constituted, and the inverted round stage hole 12 in embodiment six is substituted for chamfered edge platform hole 13, and the side in chamfered edge platform hole 13 is still arranged
Some tetrahedrons；The operation principle of the present embodiment and specific embodiment are with embodiment six.
(micropore vestibule is made up of embodiment 11 round tube hole and flared hole, and flared hole bottom surface arranges a refractive body, instead
Body of light can have multiple choices).
As shown in figure 11, the present embodiment and the difference of embodiment one are: the inverted round stage hole 12 of micropore vestibule 10 bottom
It is substituted for flared hole 14, the both big immune detection liquid of this flared hole 14 and its adsorption reaction area, can make through round tube hole again
The light of 11 downward projections is irradiated to the side ring wall of flared hole 14 and reflecting condensation；On flared hole 14 bottom surface in the present embodiment
Refractive body 20 can be one of segment 21, circular cone 22, round platform 23, pyramid 24 or terrace with edge 25, described these refractive bodies
Increase itself and immune detection liquid and its adsorption reaction area, the light being incident upon its surface can be reflexed to flared hole 14 again
On, then through flared hole 14 ring wall reflecting condensation to increase the light signal strength of detected light.
(micropore vestibule is made up of embodiment 12 round tube hole and the hole of falling spherical zone, and the bottom hole of falling spherical zone face arranges a refractive body, instead
Body of light can have multiple choices).
As shown in figure 12, the present embodiment and the difference of embodiment 11 are: the flared hole of micropore vestibule 10 bottom
14 are substituted for the hole of falling spherical zone 15, the surface in the hole of falling spherical zone 15 both big immune detection liquid and its adsorption reaction area, can make again through
The light that round tube hole 11 projects downwards is irradiated to the side ring wall in the hole of falling spherical zone 15 and reflecting condensation；Anti- on the bottom surface of the hole of falling spherical zone 15
Body of light 20 can be one of segment 21, circular cone 22, round platform 23, pyramid 24 or terrace with edge 25.
(micropore vestibule is made up of embodiment 13 round tube hole and rounding halfpace hole, and the setting one of rounding halfpace bottom hole face is reflective
Body, refractive body can have multiple choices).
As shown in figure 13, the present embodiment and the difference of embodiment 11 are: the flared hole of micropore vestibule 10 bottom
14 are substituted for rounding halfpace hole 16, and rounding halfpace hole 16 ring wall is to be mixed with line segment around its middle shaft rotation by curve and straight line
The closed surface becoming；The both big immune detection liquid in surface and its adsorption reaction area in circle halfpace hole 16, can make through round tube hole again
The light of 11 downward projections is irradiated to the side ring wall in round halfpace hole 16 and reflecting condensation；Refractive body 20 on circle halfpace hole 16 bottom surface
Can be one of segment 21, circular cone 22, round platform 23, pyramid 24 or terrace with edge 25.
Various embodiments above is used for illustrative purposes only, rather than limitation of the present invention, the common skill of the art
Art personnel, without departing from the spirit and scope of the present invention, can also make various conversion or change, therefore, own
Equivalent technical scheme also should belong to scope of the invention, is limited by each claim.
Claims (4)
1. a kind of micropore of immune detection, including a micropore vestibule it is characterised in that: described micropore vestibule is justified by top one
Through hole and bottom one have increase and connect and compose according to the cup aperture of the side ring wall with reflecting condensation area to upslide, and described cup aperture is
Inversed taper platform hole, on cup aperture bottom surface integrally formed with one have increase light to upslide according to and reflex to cup aperture side ring surface on anti-
Body of light.
2. as claimed in claim 1 a kind of micropore of immune detection it is characterised in that: described inversed taper platform hole be rotary body
Hole, described rotation body opening is inverted round stage hole, flared hole, the hole of falling spherical zone or rounding halfpace hole.
3. as claimed in claim 1 a kind of micropore of immune detection it is characterised in that: described inversed taper platform hole be chamfered edge platform
Hole.
4. as claimed in claim 1 a kind of micropore of immune detection it is characterised in that: described refractive body be a segment,
Spherical zone body, circular cone, round platform, pyramid or terrace with edge.
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| CN201510408362.9A CN104977405B (en) | 2015-07-13 | 2015-07-13 | Immunodetection micropore |
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| CN104977405B true CN104977405B (en) | 2017-01-18 |
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| CN105717289B (en) * | 2016-03-24 | 2017-11-24 | 何韶衡 | A kind of ELISA Plate |
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| CN102943033A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | 96-well cell culture plate |
| CN104034880A (en) * | 2013-12-16 | 2014-09-10 | 苏州国科鼎翼生物科技有限公司 | Reaction cup used for automatic chemical luminescence immunoassay instrument |
| CN203923172U (en) * | 2014-06-20 | 2014-11-05 | 西安交通大学 | Integral type paramagnetic particle method cellular segregation, cultivation, check-out console |
| CN204758605U (en) * | 2015-07-13 | 2015-11-11 | 徐恩良 | Micropore that immunodetection used |
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| CN104977405A (en) | 2015-10-14 |
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Effective date of registration: 20191118 Address after: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289 building third unit 10 branch Patentee after: XIAMEN XIANMING BIOTECHNOLOGY CO., LTD. Address before: Siming District Yuqing road Xiamen City, Fujian province 361003 No. 33 building B room 1302 Patentee before: Xu Enliang |