CN105717289B - A kind of ELISA Plate - Google Patents
A kind of ELISA Plate Download PDFInfo
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- CN105717289B CN105717289B CN201610173828.6A CN201610173828A CN105717289B CN 105717289 B CN105717289 B CN 105717289B CN 201610173828 A CN201610173828 A CN 201610173828A CN 105717289 B CN105717289 B CN 105717289B
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- micropore
- protrusion
- wall
- elisa plate
- hole
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- 238000002965 ELISA Methods 0.000 title abstract description 35
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims 1
- 238000003491 array Methods 0.000 description 12
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 239000013076 target substance Substances 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229920002223 polystyrene Polymers 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 241001141491 Eumorpha elisa Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses a kind of ELISA Plate, can include：Reactive tank and at least one micropore；The micropore includes micropore outer wall and protrusion, the micropore outer wall is placed on the bottom plate of the reactive tank, the protrusion is placed in the micropore, it is connected with the bottom plate, the protrusion is raised with the micropore concentric tapered tubular, or the protruding block that the protrusion is distributed by multiple tapered tubulars is formed, the conically shaped and the micropore that the multiple protruding block is formed are concentric.A kind of bottom that the present invention protects is the ELISA Plate with hole concentric circles platform structure, adds the surface area of reaction.
Description
Technical field
The present invention relates to the reaction enzymes field of immune detection in bioscience research field or medical diagnosis, particularly relate to
A kind of and ELISA Plate.
Background technology
Immune detection be life science it is most common detection target molecule method, EUSA
(ELISA) be immune detection important component.ELISA is broadly divided into two kinds of reactions：A kind of is between antigen and antibody
Immune response, another kind are biomolecule and the solid phase surface adsorbed.
In EUSA (ELISA), antigen, antibody, labelled antibody or the antigen of immunological response are participated in
Purity, concentration and ratio；The condition such as buffer solution species, concentration and ionic strength, pH value and reaction temperature, time plays key
Effect.In addition, absorption of the ELISA Plate surface as carrier to antigen, antibody or antigen antibody complex also play it is extremely important
Effect.
In the prior art, the most frequently used material of ELISA ELISA Plates is polystyrene.In recent years, in order to increase biomolecule
Affinity with solid phase surface, the sensitivity for improving reaction, strengthen stability of detection etc., the selection of ELISA Plate solid phase material and
Processing causes the very big concern of this area researcher.At present, this area researcher is directed to research by covalently handing over more
The methods of joining chemical group activating functional groups, chemical reaction modification polystyrene surface and ultraviolet irradiation changes polystyrene table
The chemical property in face, and then affinity of the ELISA Plate hole surface to biomolecule is improved, and have been achieved for significant progress.
Asked however, polystyrene ELISA Plate still suffers from another problem surface area that i.e. ELISA Plate hole is reacted with biomolecule
Topic.ELISA Plate of the prior art is most for flat (structure such as Figure of description 1), U-shaped bottom or V-type bottom, flat refractive index
It is low, detected suitable for ELIASA, the ELISA Plate refractive index of U-shaped bottom is higher, convenient sample-adding, inhales the operation such as sample, mixing, the enzyme mark at V bottoms
Plate can accurate pipette samples.But the surface area of the ELISA Plate hole of above-mentioned several ELISA Plates and biomolecule reaction compared with
It is small, it is unfavorable for the abundant quick progress of reaction.
The content of the invention
It is an object of the invention to provide a kind of ELISA Plate, and what the solid phase surface adsorbed with solving above-mentioned biomolecule accumulated asks
Topic, increases response area.
To achieve the above object, the invention provides following scheme：The application provides a kind of ELISA Plate, can include：Instead
Answer groove and at least one micropore；The micropore includes micropore outer wall and protrusion, and the micropore outer wall is placed in the reactive tank
On bottom plate, the protrusion is placed in the micropore, is connected with the bottom plate, and the protrusion is and the micropore concentric tapered
Tubular is raised, or the protruding block that the protrusion is distributed by multiple tapered tubulars is formed, the cone that the multiple protruding block is formed
Shape cylinder and the micropore are concentric.
Optionally, the protrusion is made up of the protruding block of every three tapered tubulars distributions of circle, highly for 0.2~
5.0mm, wall thickness are 0.1~1.0mm, have three holes between three protruding blocks, and each width is 0.1~2.0mm.
Optionally, the conically shaped concentric structure that 3~5 circles are formed by three protrusions is disposed with the micropore,
The diameter for the concentric tapered cylinder that three protrusions are formed is different, respectively 0.2~2.0mm, 0.6~4.0mm, 1~
6.0mm, 1.5~8.0mm, 2.0~10mm.There are hole, each width between any two protrusion in three protrusions
For 0.1~2.0mm.
Optionally, the micropore outer wall is higher than the protrusion.
Optionally, the micropore outer wall is divided into two parts, top up and down and is divided into cylindrical portion, and radius is 0.1~5.0mm, wall
Thickness is 0.1~1.0mm, is highly 0.1~20mm；Bottom is divided into pars infundibularis wide at the top and narrow at the bottom, the upper shed of the pars infundibularis
Radius is identical with the radius of the cylindrical portion, is 0.1~5.0mm, and lower port radius are 0.1~4.0mm, and wall thickness is 0.1~
1.0mm.The height of pars infundibularis is 0.2~6.0mm.The under shed of the pars infundibularis is connected with the reaction trough floor.
Optionally, the micropore is uniformly arranged in the reaction trough floor in matrix array, is 1~500.It is optional
, the upright projection and adjacent cells of each lateral wall of the micropore and the reaction trough floor are non-intersect.
Optionally, the reactive tank includes bottom plate and side wall, and the relatively described side wall in the side of trough floor four of reacting is to extension
Stretch a part.
Optionally, the protrusion is made up of transparent or semitransparent material.
Optionally, described ELISA Plate, it is characterised in that the area of ELISA Plate is 10~200 × 5~200mm2 enzyme marks
Plate, include 1~500 ELISA Plate micropore on each ELISA Plate.
The present invention sets the purpose of protrusion to be the surface area for increasing micropore, increases the target of target substance and solid phase surface
The chance of molecule contacts, while less sample can be used, detect the content of high concentration target substance.
Hole of the present invention is arranged on the wall of tapered protrusion thing, and described hole runs through the rip cutting of whole protrusion
Face.Therefore, the hole that the present invention is set can be such that liquid is flowed freely inside micropore, make target substance fully anti-with target molecule
Should.
The pars infundibularis of micropore bottom of the present invention, pars infundibularis are the buffer unit on micropore bottom and top.
Protrusion wall of the present invention is the hollow tubular structure for having certain altitude and thickness, and protrusion wall can prevent micropore
Between interfere.
According to specific embodiment provided by the invention, the invention discloses following technique effect：One kind that the present invention protects
Bottom is the ELISA Plate with hole concentric circles platform structure, adds the surface area of reaction, while is reduced mutually dry between micropore
Disturb.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to institute in embodiment
The accompanying drawing needed to use is briefly described, it should be apparent that, drawings in the following description are only some implementations of the present invention
Example, for those of ordinary skill in the art, without having to pay creative labor, can also be according to these accompanying drawings
Obtain other accompanying drawings；
Fig. 1 is the stereogram of traditional ELISA Plate；
Fig. 2 is the top view (Fig. 2A) and front view (Fig. 2 B) of the microcellular structure of the present invention；
Fig. 3 is the stereogram of the microcellular structure of the present invention；
Fig. 4 A-1 are the top views of 20 hole elisa Plates of 4 × 5 rectangular arrays of the present invention；
Fig. 4 A-2 are the side views of 20 hole elisa Plates of 4 × 5 rectangular arrays of the present invention；
Fig. 4 B-1 are the top views of 28 hole elisa Plates of 4 × 7 rectangular arrays of the present invention；
Fig. 4 B-2 are the side views of 28 hole elisa Plates of 4 × 7 rectangular arrays of the present invention；
Fig. 5 is 20 hole elisa Plates of 4 × 5 rectangular arrays or 28 hole enzyme marks of 4 × 7 rectangular arrays of the present invention
The a-a sectional views of plate；
Fig. 6 A and Fig. 6 B are 20 hole elisa Plates and 4 × 7 rectangular arrays row of 4 × 5 rectangular arrays of the present invention respectively
The stereogram of 28 hole elisa Plates of row；
1- micropores；2- micropore outer walls；3- protrusions；4- cylindrical portions；5- pars infundibularises；6- protruding blocks；7- holes；8- reactive tanks
Bottom plate；9- reacts groove sidewall.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
It is an object of the invention to provide a kind of ELISA Plate, to solve the above-mentioned problems of the prior art, makes response area
Increase.
In order to facilitate the understanding of the purposes, features and advantages of the present invention, it is below in conjunction with the accompanying drawings and specific real
Applying mode, the present invention is further detailed explanation.
The application provides a kind of ELISA Plate, can include structure as shown in Figure 3：Reactive tank and at least one micropore 1；Institute
Stating micropore 1 includes micropore outer wall 2 and protrusion 3, and the micropore outer wall 2 is placed on the bottom plate of the reactive tank, the protrusion 3
It is placed in the micropore, is connected with the bottom plate, the protrusion 3 is raised or described with the micropore concentric tapered tubular
Protrusion is made up of the protruding block 6 of multiple tapered tubular distributions, the conically shaped that the multiple protruding block 6 is formed and the micropore
With one heart.
Optionally, the protrusion 3 is made up of the protruding block 6 of three tapered tubular distributions, between three protruding blocks 6
With three holes 7.
Optionally, the conically shaped concentric structure that 3~5 circles are formed by three protrusions is disposed with the micropore 1
Composition, the conically shaped that three protrusions are formed is concentric and the diameter of the conically shaped of formation is different, respectively 0.2~2.0mm,
0.6~4.0mm, 1~6.0mm, 1.5~8.0mm, 2.0~10mm, it is highly 0.2~5.0mm, wall thickness is 0.1~1.0mm.Institute
The hole 7 for stating any protrusion 3 in three protrusions is relative with the middle part of the protruding block 6 of adjacent protrusion thing 3.
Optionally, the micropore outer wall is higher than the protrusion.
Optionally, the micropore outer wall is divided into two parts, top up and down and is divided into cylindrical portion 4, and radius is 0.1~5.0mm, wall
Thickness is 0.1~1.0mm, is highly 0.1~20mm；Bottom is divided into pars infundibularis 5 wide at the top and narrow at the bottom, the upper shed of the pars infundibularis
Radius is identical with the radius of the cylindrical portion, is 0.1~5.0mm, and lower port radius are 0.1~4.0mm, and wall thickness is 0.1~
1.0mm.The height of pars infundibularis is 0.2~6.0mm.The under shed of the pars infundibularis is connected with the reaction trough floor 8.
Optionally, the micropore 1 is uniformly arranged in the reaction trough floor 8 in matrix array.
Optionally, each lateral wall of the micropore 1 and upright projection and the adjacent cells of the reaction trough floor 8 are not
It is intersecting.
Optionally, the protrusion is made up of transparent or semitransparent material.
Optionally, described ELISA Plate, it is characterised in that the area of ELISA Plate is 10~200 × 5~200mm2 enzyme marks
Plate, include 1~500 ELISA Plate micropore on each ELISA Plate.
ELISA Plate provided by the invention is described in detail with reference to embodiment, but they can not be interpreted as
Limiting the scope of the present invention.
Embodiment 1
As shown in Figures 2 and 3, a kind of protrusion with hole by inside micropore, micropore bottom are present embodiments provided
Pars infundibularis and micropore top three, wall of protrusion part composition microcellular structures, this microcellular structure is that one kind can increase instead
Answer the microcellular structure of surface area.
Embodiment 2
As shown in Fig. 2 it is provided with equally distributed three inwall arc length L on the outer wall of each taper tubular protrusion1=
0.5mm hole, hole run through the whole vertical section of taper tubular protrusion.Hole can allow liquid free inside micropore
Flowing.
Embodiment 3
As shown in Figure 5, Figure 6, this example provides 20 hole elisa Plates and 4 × 7 rectangular arrays of 4 × 5 rectangular arrays
28 hole elisa Plates of arrangement, in addition to the matrix arrangement of micropore is different, the microcellular structure of 20 hole elisa Plates and 28 hole elisa Plates is complete
It is identical.
Embodiment 4
As shown in Figure 5, there is provided 20 hole elisa Plates of 4 × 5 rectangular arrays and 28 holes of 4 × 7 rectangular arrays
The diameter of the taper tubular protrusion of ELISA Plate micropore bottom is respectively set to φ1=4.0mm, φ2=3.0mm, φ3=2.0mm
And φ4=1.0mm, outermost taper tubular protrusion thickness are arranged to δ 1=0.2mm, the thickness of other taper tubular protrusions
It is disposed as δ2=0.15mm, the height of taper tubular protrusion are disposed as h1=0.8mm, taper tubular protrusion and enzyme mark
The angle at plate plate bottom is arranged to α=67.38 °.
The pars infundibularis of micropore bottom is hollow round-like structure, and the height of pars infundibularis is taper tubular protrusion height
Half, i.e. h2=0.4mm.
The protrusion wall on micropore top is tubular structure, and the height for protruding wall is arranged to 5/4ths of micropore bottom level,
That is h3=1.0mm.
The microcellular structure surface area calculation formula of embodiment 4：
The surface area of the flat micropore of tradition
The surface area of the micropore madial wall of embodiment 4 and
The hole of the taper tubular protrusion side wall of embodiment 4 reduction surface area
Surface area that the micropore lateral wall of embodiment 4 is projected perpendicular to ELISA Plate plate bottom and
The total surface area of the micropore of embodiment 4
The total surface area S of the micropore of embodiment 4AlwaysWith conventional microporous surface area SFlat microporeRatio be
The volume calculations formula of micropore described in embodiment 4：
The volume of outermost micropore
The volume of other taper tubular protrusions
The volume of hole
The volume of micropore
Embodiment 5
As shown in Fig. 4 A-1 and Fig. 4 B-1, the enzyme mark version of the invention protected, to the array distribution in reaction trough floor
Micropore carry out digital number successively, wherein among a row micropore label be respectively "-", "+", " ++ " and " +++ ".Micropore is entered
Line number, facilitate observation, the record of enzyme reaction, can clearly in comparative analysis difference numbering micropore enzyme reaction difference.
The present invention sets the purpose of tapered protrusion thing to be the surface area for increasing micropore, increases target substance and solid phase surface
Target molecule contact chance, while can use less sample, the content of detection high concentration target substance.
Hole of the present invention is arranged on the wall of tapered protrusion thing, and described hole is vertical through whole round platform with one heart
Section.Therefore, the hole that the present invention is set can be such that liquid is flowed freely inside micropore, target substance is filled with target molecule
Divide reaction.
The pars infundibularis of micropore bottom of the present invention, pars infundibularis are the buffer unit on micropore bottom and top.
Protrusion wall of the present invention is the hollow tubular structure for having certain altitude and thickness, and protrusion wall can prevent micropore
Between interfere.
According to specific embodiment provided by the invention, the invention discloses following technique effect：One kind that the present invention protects
Bottom is the ELISA Plate with hole concentric circles platform structure, adds the surface area of reaction, while is reduced mutually dry between micropore
Disturb.
Each embodiment is described by the way of progressive in this specification, what each embodiment stressed be and other
The difference of embodiment, between each embodiment identical similar portion mutually referring to.For system disclosed in embodiment
For, because it is corresponded to the method disclosed in Example, so description is fairly simple, related part is said referring to method part
It is bright.
Specific case used herein is set forth to the principle and embodiment of the present invention, and above example is said
It is bright to be only intended to help the method and its core concept for understanding the present invention；Meanwhile for those of ordinary skill in the art, foundation
The thought of the present invention, in specific embodiments and applications there will be changes.In summary, this specification content is not
It is interpreted as limitation of the present invention.
Claims (6)
- A kind of 1. ELISA Plate, it is characterised in that：Including：Reactive tank and at least one micropore (1)；The micropore (1) includes micropore Outer wall (2) and protrusion (3), the micropore outer wall (2) are placed on the bottom plate of the reactive tank, and the protrusion (3) is placed in institute State in micropore, be connected with the bottom plate, the protruding block (6) that the protrusion (3) is distributed by three tapered tubulars is formed, described The conically shaped and the micropore that three protruding blocks (6) are formed are concentric；There are three holes (7) between three protruding blocks (6)；Institute The inwall arc length for stating hole (7) is 0.5mm；The micropore (1) is uniformly arranged in the reaction trough floor (8) in matrix array On, the array arrangement carries out digital number successively in the micropore (1) on the reaction trough floor (8)；The interior cloth of the micropore (1) Be equipped with three protrusions (3), the conically shaped that three protrusions (3) are formed with one heart and the radius of conically shaped that is formed not Together, in three protrusions (3) hole (7) of any protrusion (3) with the protruding block (6) of adjacent protrusion thing (3) Portion is relative.
- A kind of 2. ELISA Plate according to claim 1, it is characterised in that：The micropore outer wall is higher than the protrusion.
- A kind of 3. ELISA Plate according to claim 1, it is characterised in that：The micropore outer wall is divided into two parts up and down, on Part is drum portion (4), and bottom is divided into pars infundibularis wide at the top and narrow at the bottom (5), the radius of the upper shed of the pars infundibularis (5) with it is described The radius in drum portion (4) is identical, and the under shed of the pars infundibularis (5) is connected with the reaction trough floor (8).
- A kind of 4. ELISA Plate according to claim 1, it is characterised in that：Each lateral wall of the micropore (1) and described anti- Answer upright projection and the adjacent cells of trough floor (8) non-intersect.
- A kind of 5. ELISA Plate according to claim 1, it is characterised in that：The reactive tank includes bottom plate (8) and side wall (9), the reaction relatively described side wall (9) in the side of trough floor (8) four stretches out a part.
- 6. ELISA Plate according to any one of claim 1 to 5, it is characterised in that the protrusion is by transparent or semi-transparent Bright material is made.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610173828.6A CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
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|---|---|---|---|
| CN201610173828.6A CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
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| CN105717289A CN105717289A (en) | 2016-06-29 |
| CN105717289B true CN105717289B (en) | 2017-11-24 |
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| CN201610173828.6A Active CN105717289B (en) | 2016-03-24 | 2016-03-24 | A kind of ELISA Plate |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106198960A (en) * | 2016-07-08 | 2016-12-07 | 何韶衡 | A kind of ELISA Plate freely assembled |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5679310A (en) * | 1995-07-11 | 1997-10-21 | Polyfiltronics, Inc. | High surface area multiwell test plate |
| CN2760557Y (en) * | 2004-12-21 | 2006-02-22 | 上海荣盛生物技术有限公司 | Elisa plate with adsorptive force |
| CN101533009B (en) * | 2009-04-15 | 2015-07-08 | 徐恩良 | Micropore structure of microplate strip |
| CN202693587U (en) * | 2012-08-13 | 2013-01-23 | 沃克(天津)生物科技有限公司 | Reaction plate without measuring light transmittance |
| CN104977405B (en) * | 2015-07-13 | 2017-01-18 | 徐恩良 | Immunodetection micropore |
| CN205506833U (en) * | 2016-03-24 | 2016-08-24 | 何韶衡 | Elisa plate |
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Effective date of registration: 20210310 Address after: 110027 no.36-3, Huahai Road, Shenyang Economic and Technological Development Zone, Liaoning Province Patentee after: SHENYANG HUIMINYUAN BIOTECHNOLOGY Co.,Ltd. Address before: 121000 35-97, jinxiutiandi District D, Lingnan Xili, Taihe District, Jinzhou City, Liaoning Province Patentee before: He Shaoheng |
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Denomination of invention: A type of enzyme-linked immunosorbent assay plate Effective date of registration: 20231225 Granted publication date: 20171124 Pledgee: Shenyang Science and Technology Venture Capital Co.,Ltd. Pledgor: SHENYANG HUIMINYUAN BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023210000355 |
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