CN104977405A - Immunodetection micropore - Google Patents
Immunodetection micropore Download PDFInfo
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- CN104977405A CN104977405A CN201510408362.9A CN201510408362A CN104977405A CN 104977405 A CN104977405 A CN 104977405A CN 201510408362 A CN201510408362 A CN 201510408362A CN 104977405 A CN104977405 A CN 104977405A
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- hole
- micropore
- vestibule
- immune detection
- cup aperture
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Hematology (AREA)
- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses an immunodetection micropore, which comprises a micropore cavity. The micropore cavity is connected by a round through-hole at the upper part of the micropore cavity and a cup hole, provided with an annular side wall which can increase an upwards projected and reflected concentrated light area, at the lower part of the micropore cavity. A reflector which can increase the upwards projected and reflected concentrated light area is projected from the bottom and/or the annular side wall of the cup hole. According to the cup hole at the lower part of the micropore cavity, an interfacial area of a solid-liquid reaction of a detected liquid is increased, so that an immunoreaction, a luminous reaction, or an enzymic catalytic reaction can be fully carried out, and the detection sensitivity is improved; and at the same time, the upwards projected and reflected concentrated light area of the interfacial area of the solid-liquid reaction is increased, so that the interfacial area of the solid-liquid reaction can be effectively illuminated by projected light, and can reflect and converge the light; a photomultiplier tube (PMT) probe of a detection device can receive more light signals from the interfacial area of the solid-liquid reaction; and the detection sensitivity is greatly improved.
Description
Technical field
The present invention relates to a kind of micropore of immune detection, specifically a kind of microcellular structure improving the microwell plate of immune detection reaction rate and sensitivity in bio-science research or medical diagnosis.
Background technology
In life science, medical research and medical diagnosis activity, based on the enzyme linked immunosorbent assay of microwell plate and being widely used of chemiluminescence immunoassay detection experiment.For improving immune detection reaction rate and sensitivity, general method adopts surface treatment, if the modes such as r radiation exposure, Ultraviolet radiation, Cement Composite Treated by Plasma are to strengthen its adsorptive power, but improves limitation.In addition, by increasing the surface area of the micropore inner chamber of microwell plate, the reaction area of reactant liquor solid-liquid interface being increased, improving detection sensitivity, shorten detection time.In the microcellular structure of existing microwell plate (bar), although solid and liquid interface (adsorption area of test solution) can be increased to improve adsorption plane but to detect for luminescence by the concavo-convex process in micropore, existing microcellular structure also fails to make solid-liquid interface light in electrochemiluminescent immunoassay detection throw according to useful area increase, the signal intensity of the reflected light at solid and liquid interface is more weak to cause detecting instrument to detect, therefore detection sensitivity is low.
Summary of the invention
The present invention is intended to the micropore proposing a kind of immune detection, has both increased immune detection solid and liquid interfacial area, increases again the effective irradiated area to upslide photograph and reflecting condensation, the reaction rate of adsorption liquid immune detection and sensitivity are improved greatly.
For reaching above-mentioned purpose, the invention provides a kind of micropore of immune detection, comprise a micropore vestibule, it is characterized in that: described micropore vestibule is had the side ring wall of upwards reflecting condensation cup aperture by top one round tube hole and bottom one connects and composes, and the bottom surface of described cup aperture and/or side ring wall are provided with the refractive body that light is upwards reflected projectedly.
Described cup aperture is inversed taper platform hole; The sidewall of the domain in inversed taper platform hole by cup aperture mouth at the bottom of cup aperture, centroclinal dip, relative to traditional vertical cyclindrical shape micropore, the setting in inversed taper platform hole had both increased immune detection solid and liquid interfacial area, again effective irradiated area at increasing light projection solid and liquid interface.
Described inversed taper platform hole is for rotating body opening, and described rotation body opening is inverted round stage hole, flared hole, the hole of falling spherical zone or rounding halfpace hole; The whole ring wall of the cup aperture of rotary body cellular type is cambered surface, and this cambered surface is for increasing immune detection solid and liquid interfacial area, and increasing light is upwards projected to effective irradiated area of photomultiplier again.
Described inversed taper platform hole is chamfered edge platform hole; The whole ring wall of cup aperture of chamfered edge platform cellular type is made up of the faceted pebble of whole circle, and downward intilted faceted pebble is upwards projected to effective irradiated area of photomultiplier for increasing immune detection solid and liquid interfacial area and increasing light.
One-body molded on described cup aperture bottom surface have one can light reflection to the refractive body on the side ring surface of cup aperture, and described refractive body is a segment, spherical zone body, circular cone, round platform, pyramid or terrace with edge; Because the refractive body of cup aperture bottom surface protrudes, the solid-liquid interface area of immune detection liquid and cup aperture bottom surface is increased, again can increasing light projection solid and liquid interface effective irradiated area, and the light projecting refractive body on the side ring surface of cup aperture, enters the optically focused intensity of the side ring surface of an increase step cup aperture through its scattered reflection.
One-body molded on described cup aperture side ring wall have some described refractive bodies, and described refractive body is the tetrahedron that bottom surface is connected with cup aperture bottom surface; Make cup aperture side ring wall and on refractive body with detect the contact area of liquid and greatly increase, and the light increasing projection micropore all can be irradiated to cup aperture side ring wall and on refractive body surface.
After technique scheme, the solid-liquid interface area of the cup aperture of immune response liquid and luminescence-producing reaction liquid and micropore vestibule bottom increases, the reaction area of reactant liquor solid-liquid interface is increased greatly, is conducive to immune response, luminescence-producing reaction fully, fast completes, Reaction time shorten; The light that the technical scheme formed can make luminescence-producing reaction project gathers photomultiplier (PMT) probe shining detecting instrument to upslide, and detecting instrument is detected, and the luminous signal strength-enhanced at solid and liquid interface is to improve detection sensitivity.
Beneficial effect of the present invention is as follows:
1. the cup aperture side ring wall of micropore vestibule bottom increases with the solid-liquid reaction interface detecting liquid, be beneficial to immune response, luminescence-producing reaction or enzymic catalytic reaction fully carry out, the time that reaction reaches balance shortens greatly, thus improves detection sensitivity, improves detection efficiency;
2. the cup aperture side ring wall of micropore vestibule bottom can make the light-emitting line of generation effectively converge with the ramp structure at the solid-liquid reaction interface detecting liquid, detecting instrument photomultiplier (PMT) is popped one's head in and receives the light signal of more solid-liquid interface, to improve detection sensitivity;
3. the cup aperture bottom surface of micropore vestibule bottom is or/and side ring surface arranges the refractive body of protrusion, refractive body increases solid-liquid vestibule bottom face or/and the solid-liquid reaction interface of side, and solid-liquid reaction interface effectively can be irradiated to by the light projected and reflecting focal, the light signal that detecting instrument photomultiplier (PMT) probe detection is arrived increases, and greatly improves the sensitivity of detection.
Accompanying drawing explanation
Fig. 1 is embodiment one structural representation of the present invention.
Fig. 2 is embodiment two structural representation of the present invention.
Fig. 3 is embodiment three structural representation of the present invention.
Fig. 4 is embodiment four structural representation of the present invention.
Fig. 5 is embodiment five structural representation of the present invention.
Fig. 6 is embodiment six structural representation of the present invention.
Fig. 7 is embodiment seven structural representation of the present invention.
Fig. 8 is embodiment eight structural representation of the present invention.
Fig. 9 is embodiment nine structural representation of the present invention.
Figure 10 is embodiment ten structural representation of the present invention.
Figure 11 is embodiment 11 structural representation of the present invention.
Figure 12 is embodiment 12 structural representation of the present invention.
Figure 13 is embodiment 13 structural representation of the present invention.
In figure, accompanying drawing is designated:
10. micropore vestibule; 11. round tube holes; 12. inverted round stage holes; 13. chamfered edge platform holes; 14. flared hole; 15. holes of falling spherical zone; 16. rounding halfpace holes;
20. refractive body; 21. segments; 22. circular cones; 23. round platforms; 24. pyramids; 25. terrace with edges; 26. tetrahedrons.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further described.
embodiment one(micropore vestibule is made up of round tube hole and inverted round stage hole, and bottom surface, inverted round stage hole arranges a segment).
As
fig. 1the micropore of shown a kind of immune detection, comprise a micropore vestibule 10, micropore vestibule 10 is had the side ring wall of upwards reflecting condensation inverted round stage hole 12 by top one round tube hole 11 and bottom one connects and composes, the bottom surface in inverted round stage hole 12 of the present invention is provided with projectedly the refractive body 20 that a light upwards reflects, refractive body 20 of the present invention adopts on the one-body molded bottom surface being connected to inverted round stage hole 12 of segment 21.
The following detailed description of the present embodiment principle of work of the present invention and effect.
As shown in Figure 1, immune detection liquid is injected in micropore vestibule 10 by round tube hole 11, the inverted round stage hole 12 of micropore vestibule 10 bottom is filled up by immune detection liquid, compared with traditional micropore, the reaction table area that the surface of inverted round stage hole 12 of the present invention side ring wall and segment 21 and immune detection liquid adsorb increases greatly, immune response is completed in the short period of time, improves detection sensitivity; And the light projected through round tube hole 11 is irradiated to the side ring surface in inverted round stage hole 12 and the surface of segment 21 completely, the side ring surface in inverted round stage hole 12 has reflecting condensation function to light, and the surface of segment 21 has function light reflection is diffused on the side ring surface in round platform hole 12, the solid-liquid interface through inverted round stage hole 12 side ring wall and segment 21 is made to reflect the light signal strength increase collected, the luminous signal of reactant is made more by force, more to concentrate on light-emitting appearance probe window position, with improve electrochemiluminescent immunoassay detect sensitivity, shorten detection time, improve detection efficiency.
Also a facet can be cut out in segment 21 top in the present embodiment and form spherical zone body, its function and effect are equal to segment 21.
embodiment two(micropore vestibule is made up of round tube hole and inverted round stage hole, and bottom surface, inverted round stage hole arranges a circular cone).
As
fig. 2shown in, the present embodiment is with the difference of embodiment one: the one-body molded circular cone 22 that is connected in the bottom surface, inverted round stage hole 12 of micropore vestibule 10 bottom is as refractive body 20, circular cone 22 surface projection light reflection thereon on the side ring wall in inverted round stage hole 12, can converge to reflected light the intensity increasing light signal by the side ring wall in inverted round stage hole 12; The conical surface of inverted round stage hole 12 ring wall and circular cone 22 had both increased micropore vestibule 10 and had also increased the luminous effective irradiated area detected with the adsorption reaction surface area of immune detection liquid, made the reaction time of immune detection liquid shorten and improve detection sensitivity; Other structure of the present embodiment and principle of work are with embodiment one.
embodiment three(micropore vestibule is made up of round tube hole and inverted round stage hole, and bottom surface, inverted round stage hole arranges a round platform).
As
fig. 3shown in, the present embodiment is with the difference of embodiment one: the one-body molded round platform 23 that is connected in the bottom surface, inverted round stage hole 12 of micropore vestibule 10 bottom is as refractive body 20, and round platform 23 side considerably increases absorption and the reaction surface surface of micropore vestibule 10 bottom surface and immune detection liquid; And by the light of round tube hole 11 projection downwards through round platform 23 offside reflection to side, inverted round stage hole 12, through side, inverted round stage hole 12 optically focused to increase the intensity of light signal; Other structure and working principle of the present embodiment is with embodiment one.
embodiment four(micropore vestibule is made up of round tube hole and inverted round stage hole, and bottom surface, inverted round stage hole arranges a pyramid).
As
fig. 4shown in, the present embodiment is with the difference of embodiment one: the one-body molded pyramid 24 that is connected in the bottom surface, inverted round stage hole 12 of micropore vestibule 10 bottom is as refractive body 20, the faceted pebble of pyramid 24 surrounding considerably increases absorption and the reaction surface surface of micropore vestibule 10 bottom surface and immune detection liquid, and by the light of round tube hole 11 projection downwards through pyramid 24 surface reflection to side, inverted round stage hole 12, through side, inverted round stage hole 12 optically focused to increase the intensity of light signal; Other structure and working principle of the present embodiment is with embodiment one.
embodiment five(micropore vestibule is made up of round tube hole and inverted round stage hole, and bottom surface, inverted round stage hole arranges a terrace with edge).
As
fig. 5shown in, the present embodiment is with the difference of embodiment one: the one-body molded terrace with edge 25 that is connected in the bottom surface, inverted round stage hole 12 of micropore vestibule 10 bottom is as refractive body 20, terrace with edge 25 side considerably increases the adsorption surface area of micropore vestibule 10 bottom surface and immune detection liquid, also to make by the light of round tube hole 11 projection downwards through terrace with edge 25 offside reflection to side, inverted round stage hole 12, through side, inverted round stage hole 12 optically focused to increase the intensity of light signal; Other structure and working principle of the present embodiment is with embodiment one.
embodiment six(micropore vestibule is made up of round tube hole and inverted round stage hole, and side, inverted round stage hole arranges some tetrahedrons).
As
fig. 6shown in, the present embodiment is with the difference of embodiment one: the side ring surface, inverted round stage hole 12 of micropore vestibule 10 bottom is one-body molded is connected some tetrahedrons 26 as refractive body 20, absorption and the reaction table area of itself and immune detection liquid is increased by tetrahedron 26 two sides, by the light of round tube hole 11 projection downwards through the offside reflection optically focused of direct irradiation inverted round stage side ring surface, hole 12 and tetrahedron 26, namely inverted round stage hole, micropore vestibule 10 bottom 12 increases greatly with the adsorption area of immune detection liquid, and inverted round stage hole 12 and the adsorption area of immune detection liquid are irradiated by light can optically focused and increase detected light signal strength, greatly to improve detection sensitivity, improve detection efficiency, other structure of the present embodiment and principle of work are with embodiment one.
embodiment seven(micropore vestibule is made up of round tube hole and chamfered edge platform hole, and bottom surface, chamfered edge platform hole arranges a segment).
As
fig. 7shown in, the difference of the present embodiment and embodiment one is: micropore vestibule 10 is made up of round tube hole 11 and chamfered edge platform hole 13, namely the inverted round stage hole 12 of micropore vestibule 10 bottom in embodiment one is replaced to chamfered edge platform hole 13, bottom surface, chamfered edge platform hole 12 still arranges the segment 21 as implemented in; Chamfered edge platform hole 13 connects and composes a domain face by the straightedge face of some inclinations, the adsorption reaction surface of chamfered edge platform hole 13 inwall and immune detection liquid is increased greatly, segment 21 also adds the adsorption reaction surface of micropore vestibule 10 bottom surface and immune detection liquid, and the side ring surface in chamfered edge platform hole 13 and the surface of segment 21 are light detection effective irradiating surface, and the light on segment 21 surface can through the side ring surface reflecting condensation in chamfered edge platform hole 13 to strengthen the intensity of luminous signal; The principle of work of other structure of the present embodiment is with embodiment one.
embodiment eight(micropore vestibule is made up of round tube hole and chamfered edge platform hole, and bottom surface, chamfered edge platform hole arranges a circular cone).
As
fig. 8shown in, the present embodiment one is with the difference of embodiment seven: the refractive body 20 of the bottom surface, chamfered edge platform hole 13 of micropore vestibule 10 bottom replaces to a circular cone 22, circular cone 22 surface projection light reflection thereon on the side ring wall in chamfered edge platform hole 13, can converge to reflected light the intensity increasing light signal by the side ring wall in chamfered edge platform hole 13; The conical surface of chamfered edge platform hole 13 ring wall and circular cone 22 had both increased micropore vestibule 10 and had also increased the luminous effective irradiated area detected with the adsorption reaction surface area of immune detection liquid, made the reaction time of immune detection liquid shorten and improve detection sensitivity.Other structure of the present embodiment and principle of work are with embodiment seven.
The circular cone 22 of the present embodiment also can be equal to the round platform 23 replaced in embodiment three.
embodiment nine(micropore vestibule is made up of round tube hole and chamfered edge platform hole, and bottom surface, chamfered edge platform hole arranges a pyramid).
As
fig. 9shown in, the present embodiment one is with the difference of embodiment seven: the refractive body 20 of the bottom surface, chamfered edge platform hole 13 of micropore vestibule 10 bottom replaces to a pyramid 24, the faceted pebble of pyramid 24 surrounding considerably increases absorption and the reaction surface surface of micropore vestibule 10 bottom surface and immune detection liquid, and by the light of round tube hole 11 projection downwards through pyramid 24 surface reflection to side, chamfered edge platform hole 13, through side, chamfered edge platform hole 13 optically focused to increase the intensity of light signal; Other structure of the present embodiment and principle of work are with embodiment seven.
In the present embodiment, micropore vestibule 10 bottom pyramid also can be equal to and replaces to a terrace with edge 25 as refractive body 20, and its function and effect are equal to this enforcement.
embodiment ten(micropore vestibule is made up of round tube hole and chamfered edge platform hole, and side, chamfered edge platform hole arranges some tetrahedrons).
As
figure 10shown in, the difference of the present embodiment and embodiment six is: micropore vestibule 10 is made up of round tube hole 11 and chamfered edge platform hole 13, namely the inverted round stage hole 12 in embodiment six is replaced to chamfered edge platform hole 13, and the side in chamfered edge platform hole 13 still arranges some tetrahedrons; The principle of work of the present embodiment and embodiment are with embodiment six.
embodiment 11(micropore vestibule is made up of round tube hole and flared hole, and flared hole bottom surface arranges a refractive body, and refractive body can have multiple choices).
As figure
11shown in, the difference of the present embodiment and embodiment one is: the inverted round stage hole 12 of micropore vestibule 10 bottom is replaced to flared hole 14, this flared hole 14 is both large immune detection liquid and its adsorption reaction area, can make again the light through round tube hole 11 projection downwards be irradiated to the side ring wall of flared hole 14 and reflecting condensation; Refractive body 20 in the present embodiment on flared hole 14 bottom surface can be segment 21, circular cone 22, round platform 23, one of pyramid 24 or terrace with edge 25, these described refractive bodies all can increase itself and immune detection liquid and its adsorption reaction area, again can being incident upon the light reflection on its surface in flared hole 14, then through flared hole 14 ring wall reflecting condensation to increase the light signal strength of detected light.
embodiment 12(micropore vestibule is made up of round tube hole and the hole of falling spherical zone, and bottom surface, the hole of falling spherical zone arranges a refractive body, and refractive body can have multiple choices).
As figure
12shown in, the difference of the present embodiment and embodiment 11 is: the flared hole 14 of micropore vestibule 10 bottom is replaced to the hole of falling spherical zone 15, the surface in the hole of falling spherical zone 15 is both large immune detection liquid and its adsorption reaction area, can make again the light through round tube hole 11 projection downwards be irradiated to the side ring wall in the hole of falling spherical zone 15 and reflecting condensation; Refractive body 20 on bottom surface, the hole of falling spherical zone 15 can be segment 21, circular cone 22, round platform 23, one of pyramid 24 or terrace with edge 25.
embodiment 13(micropore vestibule is made up of round tube hole and rounding halfpace hole, and bottom surface, rounding halfpace hole arranges a refractive body, and refractive body can have multiple choices).
As figure
13shown in, the difference of the present embodiment and embodiment 11 is: the flared hole 14 of micropore vestibule 10 bottom is replaced to rounding halfpace hole 16, rounding halfpace hole 16 ring wall be mixed by curve and straight line and line segment around the closed surface of its middle shaft rotation; Surface both large immune detection liquid and its adsorption reaction area in circle halfpace hole 16, can make again the light projected through round tube hole 11 be irradiated to the side ring wall in round halfpace hole 16 and reflecting condensation downwards; Refractive body 20 on circle bottom surface, halfpace hole 16 can be segment 21, circular cone 22, round platform 23, one of pyramid 24 or terrace with edge 25.
Each embodiment is used for illustrative purposes only above, but not limitation of the present invention, those skilled in the art, without departing from the spirit and scope of the present invention, various conversion or change can also be made, therefore, all equivalent technical schemes also should belong to category of the present invention, are limited by each claim.
Claims (6)
1. the micropore of an immune detection, comprise a micropore vestibule, it is characterized in that: described micropore vestibule by top one round tube hole and bottom one have increase to upslide according to and the cup aperture of side ring wall of reflecting condensation area connect and compose, the bottom surface of described cup aperture and/or side ring wall are provided with the refractive body increased to upslide photograph and reflecting condensation area projectedly.
2. the micropore of a kind of immune detection as claimed in claim 1, is characterized in that: described cup aperture is inversed taper platform hole.
3. the micropore of a kind of immune detection as claimed in claim 2, is characterized in that: described inversed taper platform hole is for rotating body opening, and described rotation body opening is inverted round stage hole, flared hole, the hole of falling spherical zone or rounding halfpace hole.
4. the micropore of a kind of immune detection as claimed in claim 2, is characterized in that: described inversed taper platform hole is chamfered edge platform hole.
5. the micropore of a kind of immune detection as claimed in claim 1, it is characterized in that: one-body molded on described cup aperture bottom surface have one can light reflection to the refractive body on the side ring surface of cup aperture, and described refractive body is a segment, spherical zone body, circular cone, round platform, pyramid or terrace with edge.
6. the micropore of a kind of immune detection as claimed in claim 1, is characterized in that: one-body molded on described cup aperture side ring wall have some described refractive bodies, and described refractive body is the tetrahedron that bottom surface is connected with cup aperture bottom surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510408362.9A CN104977405B (en) | 2015-07-13 | 2015-07-13 | Immunodetection micropore |
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| CN201510408362.9A CN104977405B (en) | 2015-07-13 | 2015-07-13 | Immunodetection micropore |
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| CN104977405B CN104977405B (en) | 2017-01-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105717289A (en) * | 2016-03-24 | 2016-06-29 | 何韶衡 | ELISA plate |
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| CN105717289A (en) * | 2016-03-24 | 2016-06-29 | 何韶衡 | ELISA plate |
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| CN104977405B (en) | 2017-01-18 |
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Effective date of registration: 20191118 Address after: Haicang District of Xiamen City, Fujian province 361000 Xinyang Street Weng Kok Road No. 289 building third unit 10 branch Patentee after: XIAMEN XIANMING BIOTECHNOLOGY CO., LTD. Address before: Siming District Yuqing road Xiamen City, Fujian province 361003 No. 33 building B room 1302 Patentee before: Xu Enliang |