CN104330358A - Micro-flow cell for detecting ECL (electrochemiluminescence) signal of printing electrode and application of micro-flow cell - Google Patents
Micro-flow cell for detecting ECL (electrochemiluminescence) signal of printing electrode and application of micro-flow cell Download PDFInfo
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- CN104330358A CN104330358A CN201410614813.XA CN201410614813A CN104330358A CN 104330358 A CN104330358 A CN 104330358A CN 201410614813 A CN201410614813 A CN 201410614813A CN 104330358 A CN104330358 A CN 104330358A
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Abstract
The invention discloses a micro-flow cell for detecting an ECL (electrochemiluminescence) signal of a printing electrode and the application of the micro-flow cell. The micro-flow cell mainly comprises a body, wherein a cavity is formed in the body; channels are formed in two sides of the cavity respectively; the cavity is connected with the outside by virtue of the channels; an opening is formed in one side, not provided with the channels, of the cavity; a plug is arranged at the opening correspondingly. The micro-flow cell can serve as an ECL device or an electrochemical analysis device, is simple in structure, is small in size and is portable; the consumption of reagents is low, and an output signal is sensitive.
Description
Technical field
The present invention relates to Electroanalytical Chemistry field, be specifically related to a kind of micro-flow cell and the application thereof that detect electrogenerated chemiluminescence (electrochemiluminescence, the ECL) signal that prints electrode.This device simple, sensitive, constructed detection system is few to the amount of samples of copper ion in biological sample, and analytic signal is sensitive.
Background technology
The electrolytic cell that current ECL input is suitable for mainly is applicable to common rod-shaped electrode, and opening is in upper end, and bottom is transparent corresponds to signal receiver.Electrode is vertically inserted liquid level, and the signal that luminophor produces directly is received by bottom detecting device.And detect liquid-solid fixed, can not circulate, be not easy to detect adding of sample.Because screen printing apparatus is simple, easy to operate, print, make a plate simple and easy and with low cost, strong adaptability.In recent years, screen printing technique fast development in prepared by electrode, it is low and can the advantage of batch making that electrode screen printing electrode has flexible design, cost, in laboratory study and product development, all receives increasing concern.Be used in ECL to analyze and detect case above by printing electrode and also get more and more, but the shape printed electrode determining it is not suitable for plain electrolysers, developing that a kind of to be applicable to detect print electrode micro-flow cell of ECL signal more and more urgent.
Summary of the invention
The object of this invention is to provide a kind of detection to print electrode micro-flow cell of ECL signal and application thereof.Simply portable micro-flow cell, for detect modify the ECL printed electrode; Because of the circulation in its duct, be convenient to adding of sample, and multiple sample flow monitorings of tandem order sample introduction.And micro-flow cell volume is little, portable, applicable, few with sample, solves existing electrolytic cell and to be unfavorable for printing electrode the problem of sample detection and Multi-example order sample detection.
The technical solution realizing the object of the invention is:
Detection is printed electrode the micro-flow cell of ECL signal, and mainly comprise body, body build-in cavities, cavity both sides have passage, and are connected with the external world by passage, and the non-wherein side arranging passage of cavity arranges opening, and corresponding opening is mounted with stopper.
Body in the present invention take polymethylmethacrylate as the flat rectangular body box of material.Opening is the insert port that prints electrode.The stopper inserting the opening arrangement of printing electrode corresponding to body is quality of rubber materials.In cavity both sides institute, open walk is first passage and second channel, is the import and export of flow injection liquid respectively.First passage is connected with the external world with the external flexible rubber hose of second channel.
Detection of the present invention prints electrode micro-flow cell of ECL signal for detecting the method for the ECL signal that prints electrode.Following method is method for optimizing:
A kind of detection prints electrode micro-flow cell of ECL signal for detecting the method for the ECL signal that prints electrode.Above-mentioned detection is printed electrode the method for ECL signal, is that the change by detecting ECL signal detects Cu
2+concentration, described micro-flow cell needs to be used in conjunction with flow injection injector and Electrochemiluminescprocess process system, and concrete grammar is as follows:
The first step: surface preparation of printing electrode, modifies luminescent quantum dot, waits to be dried.
Second step: downward for the workplace printed electrode entirety is inserted in the opening 3 of micro-flow cell, buckles stopper 4, only stay end copper sheet metal to connect for circuit.
3rd step: under the control of flow injection injector, will mix different Cu by feed pathway 5
2+the detection liquid of concentration injects micro-flow cell successively, not contain Cu between variable concentrations gradient
2+detection liquid rinse a period of time, liquid outlet channel 6 discharges liquid.
4th step: by the different Cu of Electrochemiluminescprocess process systems axiol-ogy record
2+the ECL signal intensity that Concentration Testing liquid is corresponding.
The present invention is that material manufactures micro-flow cell with polymethylmethacrylate, and material is tough and tensile, transparent, is easy to carry about with one, and manufacture craft is simple, cheaply.Shape is a micro-box of rectangle, and a built-in storage detects the rectangular enclosure of liquid.Cavity accounts for nearly 1/3 of whole micro-box, width with print electrode quite, therefore whole micro-flow cell volume is very little, and detection sample used is little.Insert the opening inward flange printed electrode and inlay waterproof O shape ring, additional rubber peel plug, can prevent liquid leakage.In body, cavity both sides are opened two passages and are communicated with the external world.The first passage opened is feed pathway, is connected with flow injection injector by flexible rubber hose, controls feed liquor flow in real time; Second channel is liquid outlet channel, connects flexible rubber hose, is convenient to collect trickle.By detecting ECL signal intensity to Cu
2+when concentration detects, modified luminescent quantum dot print electrode insert micro-flow cell downwards in workplace cavity in, only stay print electrode end copper sheet for wire connect.Then micro-flow cell is put into the magazine of Electrochemiluminescprocess process system, corresponding each electrode of connection, closes magazine.Micro-flow cell import flexible rubber hose stretches out outside magazine and connects flow injection injector, and outlet flexible rubber hose stretches out the external fluid collection vials of magazine.By flow injection containing different Cu
2+the detection liquid of concentration, detection record ECL luminous intensity and Cu
2+relation between concentration.
The present invention compared with prior art, has following characteristics:
This kind of detection is printed electrode, and to lead to manufacture craft easy for the miniflow of ECL signal, and simple and convenient with the use of the detection system printed electrode, signal response is sensitive, specificity is high.Sample introduction flow can be controlled by flow injection injector and realize the multiple sample flow monitorings to tandem order sample introduction.
Accompanying drawing explanation
The micro-flow cell overall schematic of Fig. 1.
Fig. 2 detects the ECL signal schematic representation that prints electrode.
The sealing schematic diagram of the rubber material that the micro-flow cell of Fig. 3 is arranged.
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment
In order to understand the present invention better, illustrate technical scheme of the present invention below by specific embodiment.
Embodiment
Detection is printed electrode the micro-flow cell of ECL signal, mainly comprises body 1, body build-in cavities 2.Cavity 2 both sides have passage, and are connected with the external world by passage.The non-wherein side arranging passage of cavity arranges opening 3, and corresponding opening is mounted with stopper 4.Body 1 take polymethylmethacrylate as the flat rectangular body box of material.Opening 3 is the insert port that prints electrode, and inside establishes rubber ring.The stopper 4 settled corresponding to opening 3 is quality of rubber materials, buckles into opening 3 and can fit like a glove, can well prevent leakage after electrode insertion.Being first passage 5 and second channel 6 at cavity both sides institute open walk, is the import and export passage of flow injection liquid respectively.First passage 5 connects flow injection injector by flexible rubber hose, and second channel 6 is connected with fluid collection vials by flexible rubber hose.
This detection being printed electrode the application of micro-flow cell of ECL signal, detecting Cu by detecting ECL signal
2+the method of concentration is as follows:
The first step: shown in Fig. 2,7 surface preparations of printing electrode, at a certain amount of quantum dot 8 having ECL signal of working electrode 9 finishing of printing electrode.After drying, the workplace printed electrode is inserted downwards in the opening 3 of above-mentioned micro-flow cell, buckle rubber stopper 4, only stay end copper sheet metal outside.
Second step: the magazine micro-flow cell being put into Electrochemiluminescprocess process system, the end copper sheet metal that prints electrode is connected with the wire of instrument.Feed liquor and fluid flexible pipe are stretched out by the aperture of magazine, connect flow injection injector and Liquid acquisition bottle respectively.
3rd step: the parameter arranging flow injection injector and Electrochemiluminescprocess process system is, sample introduction motor rotary speed: 300 μ Lmin
-1; Photomultiplier bias voltage :-800V; Number of stages of amplification: 3; Sweep speed: 100mVs
-1.
4th step: carry out flow injection sample introduction by the control of flow injection injector, different Cu will be mixed
2+the detection liquid of ion concentration injects micro-flow cell successively, rinses a period of time between variable concentrations gradient to detect liquid.Detecting liquid is the 0.1MpH8.0PBS of the saturation of the air.By the different Cu of Electrochemiluminescprocess process systems axiol-ogy record
2+the ECL signal intensity corresponding to detection liquid of ion concentration.
Claims (8)
1. a detection is printed electrode micro-flow cell of ECL signal, it is characterized in that: mainly comprise body (1), body build-in cavities (2), cavity both sides have passage, and be connected with the external world by passage, the non-wherein side arranging passage of cavity arranges opening (3), and corresponding opening is mounted with stopper (4).
2. print electrode according to the detection shown in claim 1 micro-flow cell of ECL signal, it is characterized in that, body (1) take polymethylmethacrylate as the flat rectangular body box of material.
3. detection according to claim 1 is printed electrode micro-flow cell of ECL signal, and it is characterized in that, opening (3) is the insert port that prints electrode.
4. detection according to claim 1 is printed electrode micro-flow cell of ECL signal, it is characterized in that, inserting corresponding to body (1) stopper (4) that the opening (3) that prints electrode settles is quality of rubber materials.
5. detection according to claim 1 is printed electrode micro-flow cell of ECL signal, it is characterized in that, being first passage (5) and second channel (6) at cavity both sides institute open walk, is the import and export of flow injection liquid respectively.
6. detection according to claim 5 is printed electrode micro-flow cell of ECL signal, it is characterized in that, first passage (5) is connected with the external world with second channel (6) external flexible rubber hose.
7. print electrode micro-flow cell of ECL signal for detecting the method for the ECL signal that prints electrode based on detection according to claim 1.
8. detection according to claim 7 is printed electrode the method for ECL signal, it is characterized in that, is that the change by detecting ECL signal detects Cu
2+concentration, described micro-flow cell needs to be used in conjunction with flow injection injector and Electrochemiluminescprocess process system, and concrete grammar is as follows:
The first step, surface preparation of printing electrode, modifies luminescent quantum dot, waits to be dried;
Second step, inserts downward for the workplace printed electrode entirety in the opening (3) of micro-flow cell, buckles stopper (4), only stay end copper sheet metal to connect for circuit;
3rd step, under the control of flow injection injector, will mix different Cu by feed pathway (5)
2+the detection liquid of concentration injects micro-flow cell successively, not contain Cu between variable concentrations gradient
2+detection liquid rinse a period of time, liquid outlet channel (6) discharge liquid;
4th step, by the different Cu of Electrochemiluminescprocess process systems axiol-ogy record
2+the ECL signal intensity that Concentration Testing liquid is corresponding.
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|---|---|---|---|
| CN201410614813.XA CN104330358A (en) | 2014-11-04 | 2014-11-04 | Micro-flow cell for detecting ECL (electrochemiluminescence) signal of printing electrode and application of micro-flow cell |
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| CN201410614813.XA CN104330358A (en) | 2014-11-04 | 2014-11-04 | Micro-flow cell for detecting ECL (electrochemiluminescence) signal of printing electrode and application of micro-flow cell |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223259A (en) * | 2015-09-29 | 2016-01-06 | 南京工业大学 | A kind of Portable heavy metal ion device for fast detecting and application process |
| CN107505381A (en) * | 2017-08-21 | 2017-12-22 | 中华人民共和国南京出入境检验检疫局 | Portable USB interface heavy metal ion detection device and electrode card |
| CN108152210A (en) * | 2017-11-24 | 2018-06-12 | 东南大学 | A kind of special Electrochemiluminescdetection detection cell of screen printing electrode |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11118706A (en) * | 1997-10-14 | 1999-04-30 | Hitachi Ltd | Flow through type detector |
| JP2006126092A (en) * | 2004-10-29 | 2006-05-18 | Ntt Advanced Technology Corp | Microbiosensor having thin-layer passage and dialysis membrane |
| US20080169191A1 (en) * | 2005-05-09 | 2008-07-17 | Jan Krejci | Nanostructured Working Electrode of an Electrochemical Sensor, Method of Manufacturing Thereof and Sensor Containing This Working Electrode |
| US20110284374A1 (en) * | 2009-01-20 | 2011-11-24 | Jan Krejci | Electrochemical sensor comprising a substrate and an injection moulded reaction vessel and method of its manufacture |
| CN103512938A (en) * | 2012-06-15 | 2014-01-15 | 中国科学院理化技术研究所 | Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof |
-
2014
- 2014-11-04 CN CN201410614813.XA patent/CN104330358A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11118706A (en) * | 1997-10-14 | 1999-04-30 | Hitachi Ltd | Flow through type detector |
| JP2006126092A (en) * | 2004-10-29 | 2006-05-18 | Ntt Advanced Technology Corp | Microbiosensor having thin-layer passage and dialysis membrane |
| US20080169191A1 (en) * | 2005-05-09 | 2008-07-17 | Jan Krejci | Nanostructured Working Electrode of an Electrochemical Sensor, Method of Manufacturing Thereof and Sensor Containing This Working Electrode |
| US20110284374A1 (en) * | 2009-01-20 | 2011-11-24 | Jan Krejci | Electrochemical sensor comprising a substrate and an injection moulded reaction vessel and method of its manufacture |
| CN103512938A (en) * | 2012-06-15 | 2014-01-15 | 中国科学院理化技术研究所 | Electrochemical sensor used for instantly monitoring and detecting water biotoxicity, and apparatus thereof |
Non-Patent Citations (2)
| Title |
|---|
| SHENGYUAN DENG ET AL.: "Chronopotentiometric synthesis of quantum dots with efficient surface-derived near-infrared electrochemiluminescence for ultrasensitive microchip-based ion-selective sensing", 《RSC ADVANCES》 * |
| 鞠幌先等: "量子点电致化学发光生物传感", 《分析试验室》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223259A (en) * | 2015-09-29 | 2016-01-06 | 南京工业大学 | A kind of Portable heavy metal ion device for fast detecting and application process |
| CN105223259B (en) * | 2015-09-29 | 2018-05-04 | 南京工业大学 | A kind of Portable heavy metal ion device for fast detecting and application process |
| CN107505381A (en) * | 2017-08-21 | 2017-12-22 | 中华人民共和国南京出入境检验检疫局 | Portable USB interface heavy metal ion detection device and electrode card |
| CN108152210A (en) * | 2017-11-24 | 2018-06-12 | 东南大学 | A kind of special Electrochemiluminescdetection detection cell of screen printing electrode |
| CN108152210B (en) * | 2017-11-24 | 2020-03-31 | 东南大学 | Special electrochemiluminescence detection pool for screen printing electrode |
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Application publication date: 20150204 |